CN106772993A - A kind of microscope equipment and method based on smart mobile phone and conical fiber array coupling imaging - Google Patents
A kind of microscope equipment and method based on smart mobile phone and conical fiber array coupling imaging Download PDFInfo
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- CN106772993A CN106772993A CN201611040531.9A CN201611040531A CN106772993A CN 106772993 A CN106772993 A CN 106772993A CN 201611040531 A CN201611040531 A CN 201611040531A CN 106772993 A CN106772993 A CN 106772993A
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/361—Optical details, e.g. image relay to the camera or image sensor
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
- G02B21/365—Control or image processing arrangements for digital or video microscopes
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- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Computer Vision & Pattern Recognition (AREA)
- Microscoopes, Condenser (AREA)
Abstract
The invention provides a kind of microscope equipment based on smart mobile phone and conical fiber array coupling imaging and method, belong to field of biological medicine.Design a kind of high-resolution, portable microscopic imaging device, and for processing method that further image enhaucament shows.The composite can be widely applied to blood cell pathological examination in biomedicine, can further realize that high definition is imaged, video tracking sample form, and overcome the defects such as bulky, cumbersome, the image transmitting step complexity of focusing of conventional optical microscope, have a good application prospect.
Description
Technical field
The present invention relates to field of biological medicine, one kind is more particularly related to based on smart mobile phone and conical fiber array
The microscope equipment and method of coupling imaging.
Background technology
Light microscope is widely used in biomedical detection, but its build is huge, cost is high and focusing is multiple
The defect such as miscellaneous limits the application in many fields and area, and expensive lens group considerably limit microscopical popularization.In order to
Overcome these problems, handheld, miniaturization microscope flourished in the last few years, also went to practical neck from scientific research gradually
Among domain.But these microscopes are still based on optical lens group general principle, cause optical texture excessive, focusing is complicated, it is impossible to
It is convenient to realize the defects such as teledata is shared.
The content of the invention
For above-listed demand and reality exist problem, the invention provides a kind of simple structure, enlargement ratio it is larger with
And the smart mobile phone microscopic system of simple operation, including it is a kind of aobvious based on smart mobile phone and conical fiber array coupling imaging
Microdevice, and for carrying out the image processing method of further image enhancement operation.Can be used for for intracellular in biomedicine
Liquid, and part pathogenic bacteria solution detection and diagnosis, and for demands such as subsequent remote medical treatment, the common consultation of doctors.
1st, a kind of microscope equipment based on smart mobile phone and conical fiber array coupling imaging, the system includes light transmission
Part, removably carries out the sleeve cavity of thin optical focusing.
2nd, a kind of image processing method for image enhancement operation, it includes：Based on smart mobile phone and conical fiber battle array
The microscope equipment of row coupling imaging, and for the programming in follow-up mobile phone end or server end execution for post processing
Enhancing display.
Technical scheme：
A kind of microscope equipment based on smart mobile phone Yu conical fiber array coupling imaging, the microscope equipment includes focusing one
The coupling body 6, LED of level sleeve cavity 1, specimen slide 2, the camera 4 being installed on smart mobile phone 5 and conical fiber array
Light source 8 and button cell 9；
Focusing one-level sleeve cavity 1 is fixed on the housing of the smart mobile phone 5 with built-in camera 4, one-level of focusing
Sleeve cavity 1 is configured to accommodate all optics in the way of being alignd in the illumination path of lighting source；Camera 4 is original
Optical lens group and surface optical filter figure layer remove after, by optical light glue as intermediate medium, with conical fiber array
The big end 6b of outgoing is combined as coupling body 6, coupling assembly 6 and the contact imaging of specimen slide 2；Outside focusing one-level sleeve cavity 1
End is provided with LED light source 8, is powered by button cell 9.
A kind of microscope equipment based on smart mobile phone Yu conical fiber array coupling imaging, the microscope equipment includes focusing one
Level sleeve cavity 1, specimen slide 2, the focusing two-level sleeve cavity 3, camera 4 and cone of light that are installed on smart mobile phone 5
The coupling body 6, lens or microlens array 7 of fibre array, LED light source 8 and button cell 9；
Wherein, focusing one-level sleeve cavity 1 is connected with focusing two-level sleeve cavity 3 by fine thread, two-level sleeve of focusing
Cavity 3 is fixed on the housing of the smart mobile phone 5 with built-in camera 4, focus one-level sleeve cavity 1 and focusing two-level sleeve
Cavity 3 is configured to accommodate all optics in the way of being alignd in the illumination path of lighting source；By the original light of camera 4
After lens group is removed with surface optical filter figure layer, by optical light glue as intermediate medium, the outgoing with conical fiber array
Big end 6b is combined as coupling body 6；Lens or microlens array 7, lens or microlens array are provided with focusing two-level sleeve cavity 3
7 are used to carry out the sample to be tested in specimen slide 2 in illumination path one-level amplification, and image biography after amplification is connected into coupling
Fit 6 incident small end 6a；The outer end of focusing one-level sleeve cavity 1 is provided with LED light source 8, is powered by button cell 9.
Described LED light source 8 is replaced by the LED for exciting wavelength of fluorescence, and bandpass filter is added in sleeve cavity light path,
It is applied to fluorescent microscopic imaging.
A kind of image enhancement processing method based on smart mobile phone Yu the microscope equipment of conical fiber array coupling imaging, step
It is rapid as follows：
Will enhancing Processing Algorithm be carried on smart mobile phone 5, or by by original image upload onto the server end process；
After the built-in camera of smart mobile phone 5 obtains original image, image gray-scale transformation is performed, it is therefore an objective to simplify information, retain original graph
As information profile；Feature extraction is carried out to the image after several greyscale transformations and obtains characteristic point, found by similarity measurement
The characteristic point pair matched somebody with somebody, obtains image space coordinate conversion parameter, completes image registration；Displacement is carried out to the image after matching and is folded
Plus, and equalization treatment is performed, obtain a panel height image in different resolution；Finally, to the average image filtering for obtaining, image is carried out
Storage and display.
Beneficial effects of the present invention：The present invention for conventional optical microscope is bulky, complex operation, it is expensive with
And the defect such as data sharing difficulty, propose microscope equipment based on smart mobile phone and conical fiber array coupling imaging and further
Image enhaucament display processing algorithm, and display, storage and the function such as teletransmission for realizing data are diagnosed by smart mobile phone.This hair
It is bright easily and efficiently intuitively to be strengthened display for biomedical detection sample.At the present apparatus and its enhancing display
Adjustment method has a extensive future, with universal practical value.Can be used for distance medical diagnosis or data sharing to medical data base,
In order to expert consultation and further decision-making and the plan of diagnosis scheme.
Brief description of the drawings
Fig. 1 is microscopic imaging device structure chart of the invention.
Fig. 2 is for further carrying out the Processing Algorithm that image enhaucament shows.
In figure：A imaging devices；1 focusing one-level sleeve cavity；2 specimen slides；
3 focusing two-level sleeve cavitys；4 cameras；5 smart mobile phones；6 coupling bodies；
7 lens or microlens array；8LED light sources；9 button cells.
Specific embodiment
Below in conjunction with accompanying drawing and technical scheme, specific embodiment of the invention is further illustrated.
Fig. 1 shows microscopic imaging device structure chart of the present invention on smart mobile phone 5, and smart mobile phone 5 can be
With Android, ios or windowsphone operating systems and built-in camera module mobile device.Its He of middle sleeve 1
Sleeve 3 is configured to be attached the optical delivery device lens 3, conical fiber array 6, and built-in camera 4, and links to described
The housing of smart mobile phone.
Fig. 1 imaging devices are removably focused in the cavity that sleeve constituted in two-stage, by the central point of specimen slide 2,
The coupling body 6 of lens 7, conical fiber array and mobile phone cmos image sensor attaches to one, and central optical path is alignd.Institute
State coupling body has shorter focal length and bigger enlargement ratio compared with lens.
Fig. 2 shows in detail carries out the Processing Algorithm step that image enhaucament shows.Application program is as Airborne Software by institute
State enhancing Processing Algorithm be carried on smart mobile phone 5, also can by by shooting image upload onto the server end process.In intelligent hand
After machine built-in camera obtains original image, gradation of image conversion operation is performed, for simplifying information, remain with and use image information
Profile.Feature extraction is carried out to multiple image and obtains characteristic point, the characteristic point pair of matching is found by carrying out similarity measurement, with
Image space coordinate conversion parameter is obtained, image registration is finally completed accordingly.Then image can be matched, is superimposed step behaviour
Make, and perform equalization operation obtain a panel height image in different resolution.Finally, filtering operation is performed to carry out the storage of image and show
Show.
Claims (4)
1. a kind of microscope equipment based on smart mobile phone Yu conical fiber array coupling imaging, it is characterised in that the microscope equipment
Including focusing one-level sleeve cavity (1), specimen slide (2), the camera (4) and cone of light that are installed on smart mobile phone (5)
The coupling body (6) of fibre array, LED light source (8) and button cell (9)；
Focusing one-level sleeve cavity (1) is fixed on the housing of the smart mobile phone with built-in camera (4) (5), focusing one
Level sleeve cavity (1) is configured to accommodate all optics in the way of being alignd in the illumination path of lighting source；By camera
(4) after original optical lens group is removed with surface optical filter figure layer, by optical light glue as intermediate medium, with conical fiber
The big end of outgoing (6b) of array is combined as coupling body (6), coupling body (6) and specimen slide (2) contact imaging；Coupling body can be with
Sample is amplified and imaging is completed in shooting head end；The outer end of focusing one-level sleeve cavity (1) is provided with LED light source (8), by
Button cell (9) is powered.
2. a kind of microscope equipment based on smart mobile phone Yu conical fiber array coupling imaging, it is characterised in that the microscope equipment
Including focusing one-level sleeve cavity (1), specimen slide (2), two-level sleeve cavity (3) of focusing, it is installed on smart mobile phone (5)
The coupling body (6) of camera (4) and conical fiber array, lens or microlens array (7), LED light source (8) and button cell
(9)；
Wherein, focusing one-level sleeve cavity (1) is connected with focusing two-level sleeve cavity (3) by fine thread, two-level sleeve of focusing
Cavity (3) is fixed on the housing of the smart mobile phone with built-in camera (4) (5), focus one-level sleeve cavity (1) and focusing
Two-level sleeve cavity (3) is configured to accommodate all optics in the way of being alignd in the illumination path of lighting source；Will shooting
After head (4) original optical lens group is removed with surface optical filter figure layer, by optical light glue as intermediate medium, with cone of light
The big end of outgoing (6b) of fibre array is combined as coupling body (6)；Lens or microlens array are provided with focusing two-level sleeve cavity (3)
(7), lens or microlens array (7) are put for carrying out one-level to the sample to be tested in specimen slide (2) in illumination path
Greatly, image after amplification and is passed the incident small end (6a) for being connected to coupling body (6)；Image carries out secondary putting through conical fiber array again
Greatly, complete to be imaged to camera (4) by the big end of outgoing (6b) output；The outer end of focusing one-level sleeve cavity (1) is provided with LED light source
(8), powered by button cell (9).
3. microscope equipment according to claim 1 and 2, it is characterised in that LED light source (8) is replaced by and excites wavelength of fluorescence
LED, add bandpass filter in sleeve cavity light path, be applied to fluorescent microscopic imaging.
4. a kind of image enhancement processing method based on smart mobile phone Yu the microscope equipment of conical fiber array coupling imaging, it is special
Levy and be, step is as follows：
Will enhancing Processing Algorithm be carried on smart mobile phone (5), or by by original image upload onto the server end process；In intelligence
After energy mobile phone (5) built-in camera obtains original image, image gray-scale transformation is performed, it is therefore an objective to simplify information, retain original graph
As information profile；Feature extraction is carried out to the image after several greyscale transformations and obtains characteristic point, found by similarity measurement
The characteristic point pair matched somebody with somebody, obtains image space coordinate conversion parameter, completes image registration；Displacement is carried out to the image after matching and is folded
Plus, and equalization treatment is performed, obtain a panel height image in different resolution；Finally, to the average image filtering for obtaining, image is carried out
Storage and display.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611040531.9A CN106772993A (en) | 2016-11-22 | 2016-11-22 | A kind of microscope equipment and method based on smart mobile phone and conical fiber array coupling imaging |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611040531.9A CN106772993A (en) | 2016-11-22 | 2016-11-22 | A kind of microscope equipment and method based on smart mobile phone and conical fiber array coupling imaging |
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| CN201611040531.9A Pending CN106772993A (en) | 2016-11-22 | 2016-11-22 | A kind of microscope equipment and method based on smart mobile phone and conical fiber array coupling imaging |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107607698A (en) * | 2017-10-18 | 2018-01-19 | 深圳乐普智能医疗器械有限公司 | A kind of image collecting device and there is its body fluid cell analyzer |
Citations (4)
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| CN101477233A (en) * | 2008-01-02 | 2009-07-08 | 承奕科技股份有限公司 | High-rate lens module and digital camera |
| CN103181154A (en) * | 2010-10-29 | 2013-06-26 | 加利福尼亚大学董事会 | Cellscope apparatus and methods for imaging |
| CN105629451A (en) * | 2016-01-13 | 2016-06-01 | 大连理工大学 | High resolution portable microscope system and measurement method |
| CN105954194A (en) * | 2016-04-28 | 2016-09-21 | 大连理工大学 | Light cone-based portable optical flow control type micro-imaging device and system |
-
2016
- 2016-11-22 CN CN201611040531.9A patent/CN106772993A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101477233A (en) * | 2008-01-02 | 2009-07-08 | 承奕科技股份有限公司 | High-rate lens module and digital camera |
| CN103181154A (en) * | 2010-10-29 | 2013-06-26 | 加利福尼亚大学董事会 | Cellscope apparatus and methods for imaging |
| CN105629451A (en) * | 2016-01-13 | 2016-06-01 | 大连理工大学 | High resolution portable microscope system and measurement method |
| CN105954194A (en) * | 2016-04-28 | 2016-09-21 | 大连理工大学 | Light cone-based portable optical flow control type micro-imaging device and system |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107607698A (en) * | 2017-10-18 | 2018-01-19 | 深圳乐普智能医疗器械有限公司 | A kind of image collecting device and there is its body fluid cell analyzer |
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Application publication date: 20170531 |