CN106771030A - A kind of device that toxicity test is carried out with zebra fish - Google Patents
A kind of device that toxicity test is carried out with zebra fish Download PDFInfo
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- CN106771030A CN106771030A CN201611173579.7A CN201611173579A CN106771030A CN 106771030 A CN106771030 A CN 106771030A CN 201611173579 A CN201611173579 A CN 201611173579A CN 106771030 A CN106771030 A CN 106771030A
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- zebra fish
- fish
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- zebra
- toxicity test
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/18—Water
- G01N33/186—Water using one or more living organisms, e.g. a fish
Abstract
The invention discloses a kind of device that toxicity test is carried out with zebra fish,Described device includes that zebra fish cultivates rearing-box,Experiment test device,Equipment box,Micro controller,Rhone,Damping device,The zebra fish cultivates rearing-box and is provided with raising fish jar,Camera one,Fluorescent lamp one,Feeder,Temperature controller one,Ventilation unit one,Chlorination equipment one,PH adjusting meanss,The raising fish jar bottom enters rhone by the master cylinder reclaimed water of switch valve one,The experiment test device is provided with contamination fish jar,Drainage device,Fluorescent lamp two,Camera two,Temperature controller two,Ventilation unit two,Chlorination equipment two,The contamination fish jar bottom enters rhone by the master cylinder reclaimed water of switch valve two,The equipment box is set for three layers of drawers,It is easy to glove of classifying,The equipment box upper surface be can pull case lid,The rhone is provided with outer comb mouthful.
Description
Method field
The invention belongs to environmental monitoring, and in particular to a kind of device that toxicity test is carried out with zebra fish.
Background method
The country of China's originally water resource relative shortage, population it is numerous, species enrich so that also inevitably
The need for water resource environment, but due to the special development state national conditions of China, rapid economic development for many years is caused to environment
The overdraw of resource, and due to lacking perfect environmental management system, so as to cause the deterioration of China's water resource, seriously govern me
The developing steps of state, it is achieved that the effective monitoring to environment, timely Environment control problem, are necessity of China's sustainable development
Behave.In recent years, China's water quality monitoring work achieves development well, the exploitation of monitoring device and monitoring condition in every respect
Be improved as improve environmental problem bring huge breakthrough.Animal toxicity experiment is exactly to determine pollutant opposite in the short term
Toxic action produced by thing to provide important evaluation index for environmental contaminants and pollution level.Zebra fish is a kind of common
Tropical fish, build is very thin, adult 3-4cm long, not high to water quality requirement, reaches sexal maturity within about 3 months after hatching, and ripe fish is every
Can be laid eggs once every several days, ovum is in vitro fertilization, ectogenesis, embryonic development synchronization and speed are fast, and idiosome is transparent.Zebra fishing gear
There is adult fish individuality small, good to raise, blastoprolepsis, fertility is strong, it is easy to observe, and itself and human gene have 87% height
Degree homology, the advantage as model organism is very prominent, is usually used in the monitoring of water quality environment, with scientific research value very high.But
Many experimental studies are also limited to laboratory stage at present, cumbersome, it may have significant limitation, therefore how using realizing spot
Convenient, the effectively monitoring of horse fish toxicity experiment, is not limited by laboratory condition, is exactly our problems to be solved.
The content of the invention
It is an object of the invention to provide a kind of device that toxicity test is carried out with zebra fish.
To achieve the above object, a kind of device that toxicity test is carried out with zebra fish, described device is trained including zebra fish
Rearing-box, experiment test device, equipment box, micro controller, rhone, damping device are educated, the zebra fish cultivates rearing-box and sets
There are raising fish jar, camera one, fluorescent lamp one, feeder, temperature controller one, ventilation unit one, chlorination equipment one, pH regulation dress
Put, the raising fish jar bottom enters rhone by the master cylinder reclaimed water of switch valve one, the experiment test device is set has illicit sexual relations
Malicious fish jar, drainage device, fluorescent lamp two, camera two, temperature controller two, ventilation unit two, chlorination equipment two, under the contamination fish jar
Portion enters rhone by the master cylinder reclaimed water of switch valve two, and the equipment box is set for three layers of drawers, is easy to glove of classifying, described
Equipment box upper surface be can pull case lid, the rhone is provided with outer comb mouthful.
Further, experiment test device outside position corresponding with contamination fish jar is provided with openable transparent observing
Window, convenient observation and changing water in fish tank.
Further, the sequence number corresponding to different contamination fish jars is posted at watch window upper left corner position.
Further, the chlorination equipment one and chlorination equipment two use steam spray sterilization or disinfection by ultraviolet light.
Further, the camera one and camera two are 360 ° of rotation high-definition cameras.
Further, the micro controller built-in information transport module, information storage module and message output module.
Further, described information output module includes display screen and USB interface.
Further, the equipment box be it is dismountable, it is easy to carry and use.
Further, described two cameras can set Light Source Compensation, be easy to night observation to record.
Further, the pull case lid of the equipment box can be used as experimental operation table, and simple structure is easy to use.
A kind of experimental technique of the device that toxicity test is carried out with zebra fish is：
A. it is placed on zebra fish in the zebra fish health young of 2-4cm from national zebra fish center purchase experiment height
Raised and train in cultivation rearing-box, setting the condition of raising and train is：25-27 DEG C of water temperature, pH7.3-7.9 feeds man-made feeds daily, tames and dociles
Support 15-20 days, it is ensured that its natural mortality rate is less than 3%；
B. to zebra fish starvation 24h before testing, body colour light is chosen, the active healthy zebra fish of ripple living is Experimental fish；
C. experiment is divided into multigroup with zebra fish, every group 10, is put into contamination fish jar, the prepare liquid of various concentrations is led to
Cross drainage device to add in contamination fish jar when carrying out toxotest, at least one set of blank control group is set, add distilled water, observation
The capacity and survival rate of zebra fish, calculate 24hLC50,48hLC50,72hLC50 respectively when 24h, 48h and 72h, its
In, LC50 is LC50.
The advantage of the invention is that：After a kind of device for carrying out toxicity test with zebra fish of the present invention is bought from fry
Raising and train experiment is carried out and experiment equipment outfit, realizes multifunction, and integration is intelligent, by the condition of raising and train and experiment
The control of index, obtains optimum control, so as to ensure the essence of experiment from the condition each side chosen when experiment is carried out of fry
Parasexuality.Camera can whole day 24h monitoring record zebra fish behavioural characteristic, chlorination equipment, attemperating unit, ventilation unit can be protected
Required condition is tested in confirmation, reasonable in design, simple to operate, departs from laboratory environment limitation, is suitable to promote the use of.
Brief description of the drawings
Fig. 1 is a kind of structural representation of the device that toxicity test is carried out with zebra fish；
Wherein, 1- zebra fish cultivates rearing-box；2- experiment test devices；3- equipment boxes；4- micro controllers；5- drainings
Groove；6- damping devices；7- raises fish jar；8- cameras one；9- fluorescent lamps one；10- feeders；11- temperature controllers one；12- divulges information
Device one；13- chlorination equipments one；14-pH adjusting meanss；15- contamination fish jars；16- drainage devices；17- fluorescent lamps two；18- is imaged
First two；19- temperature controllers two；20- ventilation units two；21- chlorination equipments two；22- switch valves one；23- switch valves two；24- case lids；
The outer combs mouthful of 25-；26- watch windows；27- sequence numbers.
Specific embodiment
Hereinafter, with reference to accompanying drawing 1 and embodiment, specific embodiment of the invention is described in detail：
As Fig. 1 shows a kind of device that toxicity test is carried out with zebra fish of the invention, described device includes zebra fish
Rearing-box 1, experiment test device 2, equipment box 3, micro controller 4, rhone 5, damping device 6 are cultivated, the zebra fish is cultivated and raises
Case 1 is supported to be provided with raising fish jar 7, camera 1, fluorescent lamp 1, feeder 10, temperature controller 1, ventilation unit 1, disappear
Malicious device 1, pH adjusting meanss 14, the raising bottom of fish jar 7 enter rhone 5 by the master cylinder reclaimed water of switch valve 1,
The experiment test device 2 be provided with contamination fish jar 15, drainage device 16, fluorescent lamp 2 17, camera 2 18, temperature controller 2 19,
Ventilation unit 2 20, chlorination equipment 2 21, the contamination bottom of fish jar 15 enter draining by the master cylinder reclaimed water of switch valve 2 23
Groove 5, the equipment box 3 be three layers of drawers set, be easy to classify glove, the upper surface of the equipment box 3 be can pull case lid 24,
The rhone 5 is provided with outer comb mouthful 2.
Wherein, the outside of the experiment test device 2 is provided with openable transparent observing with the corresponding position of contamination fish jar 15
Window 26；Post the sequence number 27 corresponding to different contamination fish jars 15 in the upper left corner position of the watch window 26；The chlorination equipment
1 and chlorination equipment 2 21 use steam spray sterilization or disinfection by ultraviolet light；The camera 1 and camera 2 18 are
360 ° of rotation high-definition cameras；The built-in information transport module of the micro controller 4, information storage module and message output module；Institute
Stating message output module includes display screen and USB interface；The equipment box 3 be it is dismountable, it is easy to carry and use；Described two
Individual camera can set Light Source Compensation, be easy to night observation to record；The pull case lid 24 of the equipment box can be grasped as experiment
Used as platform, simple structure is easy to use.
A kind of experimental technique of the device that toxicity test is carried out with zebra fish is：
A. it is placed on zebra fish in the zebra fish health young of 2-4cm from national zebra fish center purchase experiment height
Raised and train in cultivation rearing-box 1, setting the condition of raising and train is：25-27 DEG C of water temperature, pH7.3-7.9 feeds man-made feeds daily,
Raise and train 15-20 days, it is ensured that its natural mortality rate is less than 3%；
B. to zebra fish starvation 24h before testing, body colour light is chosen, the active healthy zebra fish of ripple living is Experimental fish；
C. experiment is divided into multigroup with zebra fish, every group 10, is put into contamination fish jar 15, by the prepare liquid of various concentrations
During by carrying out toxotest in the addition contamination fish jar 15 of drainage device 16, at least one set of blank control group being set, distilled water is added,
The capacity and survival rate of zebra fish, calculate 24hLC50,48hLC50,72hLC50 respectively during observation 24h, 48h and 72h,
Wherein, LC50 is LC50.
The present invention is not limited to above-mentioned implementation method, in the case of without departing substantially from substance of the invention, this area side
Method personnel it is conceivable that any deformation, improvement, replacement each fall within the scope of the present invention.
Claims (8)
1. a kind of device that toxicity test is carried out with zebra fish, it is characterised in that described device includes that zebra fish is cultivated and raises
Case (1), experiment test device (2), equipment box (3), micro controller (4), rhone (5), damping device (6), the zebra fish training
Educate rearing-box (1) be provided with raising fish jar (7), camera one (8), fluorescent lamp one (9), feeder (10), temperature controller one (11),
Ventilation unit one (12), chlorination equipment one (13), pH adjusting meanss (14), described raising fish jar (7) bottom pass through switch valve one
(22) master cylinder reclaimed water enters rhone (5), the experiment test device (2) be provided with contamination fish jar (15), drainage device (16),
Fluorescent lamp two (17), camera two (18), temperature controller two (19), ventilation unit two (20), chlorination equipment two (21), the contamination
Fish jar (15) bottom enters rhone (5) by the master cylinder reclaimed water of switch valve two (23), and the equipment box (3) sets for three layers of drawers
Put, be easy to glove of classifying, equipment box (3) upper surface be can pull case lid (24), the rhone (5) is provided with outer row
The mouth of pipe (25).
2. a kind of device that toxicity test is carried out with zebra fish as claimed in claim 1, it is characterised in that the experiment is surveyed
Trial assembly is put (2) outside position corresponding with contamination fish jar (15) and is provided with openable transparent observing window (26).
3. a kind of device that toxicity test is carried out with zebra fish as claimed in claim 1, it is characterised in that the observation window
Post the sequence numbers (27) corresponding to different contaminations fish jar (15) in mouth (26) upper left corner position.
4. a kind of device that toxicity test is carried out with zebra fish as claimed in claim 1, it is characterised in that the sterilization dress
Put one (13) and chlorination equipment two (21) use steam spray sterilization or disinfection by ultraviolet light.
5. a kind of device that toxicity test is carried out with zebra fish as claimed in claim 1, it is characterised in that the camera
One (8) and camera two (18), it is preferred that be 360 ° of rotation high-definition cameras.
6. a kind of device that toxicity test is carried out with zebra fish as claimed in claim 1, it is characterised in that the micro controller
(4) built-in information transport module, information storage module and message output module.
7. a kind of device of utilization zebra fish as claimed in claim 6, it is characterised in that described information output module includes aobvious
Display screen and USB interface.
8. a kind of experimental technique that toxicity test device is carried out with zebra fish as described in claim 1-7, it is characterised in that
Described experimental technique is：
A. it is placed on zebra fish cultivation in the zebra fish health young of 2-4cm from national zebra fish center purchase experiment height
Rearing-box is raised and train in (1), is set the condition of raising and train and is：25-27 DEG C of water temperature, pH7.3-7.9 feeds man-made feeds daily, tames and dociles
Support 15-20 days, it is ensured that its natural mortality rate is less than 3%；
B. to zebra fish starvation 24h before testing, body colour light is chosen, the active healthy zebra fish of ripple living is Experimental fish；
C. experiment is divided into multigroup with zebra fish, every group 10, is put into contamination fish jar (15), the prepare liquid of various concentrations is led to
Cross drainage device (16) to add when carry out toxotest in contamination fish jar (15), at least one set of blank control group is set, add distillation
Water, the capacity and survival rate of zebra fish, calculate 24hLC50,48hLC50 respectively during observation 24h, 48h and 72h,
72hLC50, wherein, LC50 is LC50.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611173579.7A CN106771030A (en) | 2016-12-16 | 2016-12-16 | A kind of device that toxicity test is carried out with zebra fish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611173579.7A CN106771030A (en) | 2016-12-16 | 2016-12-16 | A kind of device that toxicity test is carried out with zebra fish |
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| CN106771030A true CN106771030A (en) | 2017-05-31 |
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| CN201611173579.7A Pending CN106771030A (en) | 2016-12-16 | 2016-12-16 | A kind of device that toxicity test is carried out with zebra fish |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111296329A (en) * | 2020-03-03 | 2020-06-19 | 中国人民解放军第四军医大学 | Zebra fish pain sensation memory and addiction behavior experimental device and method thereof |
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| CN102899383A (en) * | 2012-11-13 | 2013-01-30 | 中国科学院华南植物园 | Application of zebra fish to testing water quality and toxicity and method for applying zebra fish to test water quality and toxicity |
| CN204269631U (en) * | 2014-12-08 | 2015-04-15 | 中国检验检疫科学研究院 | Continuous-flow type toxicity test of fishes system |
| CN105203730A (en) * | 2015-11-14 | 2015-12-30 | 林映津 | Water quality safety biological tracking and monitoring equipment |
| CN204949168U (en) * | 2015-09-24 | 2016-01-13 | 刘坤 | Multi -functional zebra fish science farming systems |
| CN105259325A (en) * | 2015-11-13 | 2016-01-20 | 南京大学 | Sewage biotoxicity online monitoring and early warning device |
| CN105684977A (en) * | 2016-03-22 | 2016-06-22 | 深圳市智汇十方科技有限公司 | Intelligent fish feeder for fish tank |
| CN105981679A (en) * | 2015-02-16 | 2016-10-05 | 南京莎菲特生物科技有限公司 | Intelligent aquarium fish tank |
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2016
- 2016-12-16 CN CN201611173579.7A patent/CN106771030A/en active Pending
Patent Citations (7)
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|---|---|---|---|---|
| CN102899383A (en) * | 2012-11-13 | 2013-01-30 | 中国科学院华南植物园 | Application of zebra fish to testing water quality and toxicity and method for applying zebra fish to test water quality and toxicity |
| CN204269631U (en) * | 2014-12-08 | 2015-04-15 | 中国检验检疫科学研究院 | Continuous-flow type toxicity test of fishes system |
| CN105981679A (en) * | 2015-02-16 | 2016-10-05 | 南京莎菲特生物科技有限公司 | Intelligent aquarium fish tank |
| CN204949168U (en) * | 2015-09-24 | 2016-01-13 | 刘坤 | Multi -functional zebra fish science farming systems |
| CN105259325A (en) * | 2015-11-13 | 2016-01-20 | 南京大学 | Sewage biotoxicity online monitoring and early warning device |
| CN105203730A (en) * | 2015-11-14 | 2015-12-30 | 林映津 | Water quality safety biological tracking and monitoring equipment |
| CN105684977A (en) * | 2016-03-22 | 2016-06-22 | 深圳市智汇十方科技有限公司 | Intelligent fish feeder for fish tank |
Non-Patent Citations (1)
| Title |
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| 国家技术监督局 等: "《中华人民共和国国家标准》", 14 September 1991 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111296329A (en) * | 2020-03-03 | 2020-06-19 | 中国人民解放军第四军医大学 | Zebra fish pain sensation memory and addiction behavior experimental device and method thereof |
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| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170531 |
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| RJ01 | Rejection of invention patent application after publication |