CN106769891B - Biochemical detection device and method - Google Patents
Biochemical detection device and method Download PDFInfo
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- CN106769891B CN106769891B CN201510811942.2A CN201510811942A CN106769891B CN 106769891 B CN106769891 B CN 106769891B CN 201510811942 A CN201510811942 A CN 201510811942A CN 106769891 B CN106769891 B CN 106769891B
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
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Abstract
A kind of biochemical detection device and method, belong to detection device and method field.This method includes: obtaining one first pixel array and one second pixel array via a photosensitive unit array;Converting first pixel array and second pixel array becomes one first color space coordinates value array and one second color space coordinates value array;Screening the first color space coordinates value array and the second color space coordinates value array according to a preset color spatial value becomes a third color space coordinate value array and one the 4th color space coordinates value array;And a color space vector array is obtained according to the third color space coordinate value array and the 4th color space coordinates value array.The present invention can be with remover itself and the various error factors of detection process and then raising biochemistry detection accuracy.
Description
Technical field
The present invention relates more specifically to a kind of biochemical detection device and method about a kind of detection device.
Background technique
Biochemical detection device uses optical detection method mostly at present, which issues detection light using light source,
It arrives at determinand and generates reflected light or transmitted light, specific wavelength model is filtered out by bandpass filter (Band pass filter)
The light of (the usually +/- 10nm of predetermined wavelength) is enclosed, then e-mail is converted light signals into photodiode (Photodiode)
Number, and compare the electronic signal of the electronic signal of determinand and zero sample under the particular range of wavelengths light, according to both electricity
The difference of subsignal can convert to obtain the information of testing concentration.
Above-mentioned detection mode must accurately control the error factor of be likely to cause deviation on optical path, such as light source,
Error factor on each assembling parts in error factor or device, can all make in the techniques such as photodiode or bandpass filter
At the error of testing result.
Moreover, the electronic signal that photodiode generates is to be radiated in its PN junction to generate by light beam, surveying
When measuring a small amount of determinand, if the determinand can not be covered uniformly in the PN junction, the electronic signal that photodiode generates will
The concentration of determinand can not be accurately reflected.
Summary of the invention
To overcome the shortcomings of existing technologies, the present invention obtains determinand using photosensitive unit array and generates reflected light or transmission
Light, while the received light information of each photosensitive unit institute is divided into different group's coloured light strength informations, such as with rgb light spectrum point
Group maps to rgb color space, and the received light information of institute can be converted into color space coordinates value array, i.e., to be measured
The aerial image information of object.
The present invention provides a kind of biochemical detection methods, includes: obtaining one first pixel array via a photosensitive unit array
And one second pixel array;Converting first pixel array and second pixel array becomes one first color space coordinates value battle array
Column and one second color space coordinates value array;The first color space coordinates value is screened according to a preset color spatial value
Array and the second color space coordinates value array become a third color space coordinate value array and one the 4th color space is sat
Scale value array;And a color is obtained according to the third color space coordinate value array and the 4th color space coordinates value array
Space vector array.
Preferably, first pixel array is generated by a zero sample image, second pixel array is by a sample to be tested
Image generates.
Preferably, also comprising obtaining a concentration array according to the color space vector array.
Preferably, first pixel array is generated by a sample to be tested image, after generating first pixel array, by one
Prefixed time interval, second pixel array are generated by the sample to be tested image.
Preferably, also comprising screening the color space vector array according to a preset color space vector to obtain one effectively
Color space vector array, and an effective concentration array is obtained according to effective color space vector array.
In addition, the present invention provides a kind of biochemical detection device, include: a light source generates a light;One transparent sample slot,
It accommodates a determinand and receives the light to generate a sample slot image;One photosensitive unit array receives and converts the sample slot
Image is a pixel array;And an image process unit, the pixel array is received, and the pixel array is converted to one first
Color space coordinates value array, and the first color space coordinates value battle array is screened according to one first preset color space coordinate threshold value
Column, to form one second color space coordinates value array.
Preferably, the image process unit is according to one first preset color space coordinate value array and second color space
Coordinate value array obtains a color space vector array.
Preferably, the image process unit obtains a testing concentration array according to the color space vector array.
Preferably, the light source is a wideband radiant.
Preferably, the light source is a white light source.
Preferably, the sample slot image is formed by a transmitted light of the determinand.
Preferably, the sample slot image is formed by a reflected light of the determinand.
Preferably, also comprising coupling an auxiliary photodiode of the image process unit with receive in the sample slot should be to
A transmitted light of object is surveyed, and generates a photosignal, which receives and convert the photosignal as a complementary colors
Color space coordinate value scale.
Preferably, should also in the sample slot to receive comprising coupling an auxiliary photosensitive unit array of the image process unit
One transmitted light of determinand, and a primary color space coordinate value array is generated, which receives the initial color
Space coordinate value array, and an auxiliary color space coordinate value array is generated according to the primary color space coordinate value array.
Furthermore the present invention provides a kind of biochemical detection device, includes: a light source can produce a light;One transparent sample
Slot has a reagent, and accommodates a determinand and receive the light to generate one first sample slot image, the reagent make this to
It surveys object and generates a color change after a specified time interval, to form one second sample slot image;One photosensitive unit array, connects
It receives and converts the first sample slot image as one first pixel array, and receive and convert the second sample slot image as one second
Pixel array;And an image process unit, first pixel array and second pixel array are received, by the first pixel battle array
Column are converted to one first color space coordinates value array, which is converted to one second color space coordinates value battle array
Column, and the first color space coordinates value array and second color space are screened according to one first preset color spatial value
Coordinate value array, to form a third color space coordinate value array and one the 4th color space coordinates value array, further according to this
Third color space coordinate value array and the 4th color space coordinates value array obtain a color space vector array.
Preferably, the image process unit screens the color space vector array according to a preset color space vector to take
An effective color space vector array is obtained, and an effective testing concentration battle array is obtained according to effective color space vector array
Column.
It is arranged preferably, the image process unit converts the color space vector array into a color space scalar matrix, and root
The color space scale array is screened according to a preset color space scale to obtain an effective color space scale array, and according to
Effective color space scale array obtains an effective testing concentration array.
The present invention can accurately select wherein effective aerial image by the aerial image information of acquirement determinand
Information is avoided because detection light is by after the determinand of non-uniform Distribution, photodiode photosurface absorb it is non-uniform to
The error surveying object transmitted light or reflected light and generating, more can analyze processing via such optical image reduces because of technique and assembling
Caused error, and then accuracy is promoted, while improving the production yield of biochemical detection device and reducing cost.
Detailed description of the invention
Other features of the invention and technical effect with reference to the accompanying drawings, and are clearly appeared from embodiment, in which:
Fig. 1 is the configuration schematic diagram of a preferred embodiment of the present invention;
Fig. 2 is the configuration schematic diagram of another preferred embodiment of the present invention;
Fig. 3 is rgb color space schematic diagram;
Schematic diagram when Fig. 4 is existing detection device determinand volume deficiency;
Schematic diagram when Fig. 5 is determinand volume deficiency in the present invention;
Schematic diagram when Fig. 6 is determinand uneven thickness in the present invention;
Fig. 7 is the configuration schematic diagram for the preferred embodiment that the present invention has a photodiode;
Fig. 8 is the configuration schematic diagram for the preferred embodiment that the present invention has an auxiliary photosensitive unit array;
Fig. 9 (a) is transparent sample slot image as acquired by photosensitive unit array of the present invention, and wherein the transparent sample slot has been
The coated blood glucose reagent that color reaction is generated to blood glucose;
Fig. 9 (b) is determinand full of above-mentioned transparent sample slot, image when color reaction is initial;
Fig. 9 (c) is image when color reaction enters stable state;
The decaying of rgb signal intensity, color difference intensity increase the relationship with the reaction time when Figure 10 is present invention detection blood glucose
Figure.
Description of symbols:
11....... light source 42....... transparent sample slot
12....... incident light 43....... determinand
13....... coupons 51....... photosensitive unit array
14....... transparent sample slot 52....... photosensitive unit
15....... transmitted light 61....... sample slot
16....... photosensitive unit array 62....... photosensitive unit array
21....... light source 63........ photosensitive unit
22....... the region of incident light 64........ deficiency standard thickness
23....... coupons 65........ reaches the region of standard thickness
24....... transparent sample slot 71........ transmitted light
25....... reflected light 72........ photodiode
26....... the photosurface of photosensitive unit array 73........ photodiode
41....... the photosurface 81........ of photodiode assists photosensitive unit array
A........ the photosensitive unit sufficiently covered
B........ insufficient photosensitive unit is covered
C........ the photosensitive unit of part covering
D........ the photosensitive unit covered by standard thickness determinand
F........ the photosensitive unit covered by thickness deficiency determinand
Specific embodiment
The present invention provides a kind of biochemical detection device, and one configuration preferably implemented is as shown in Figure 1,11 pairs of light source placements
Determinand in transparent sample slot 14 emits an incident light 12, after which penetrates determinand, the transmitted light 15 of generation
It is received by the photosensitive unit array 16 of 13 lower section of coupons and generates the pixel array for being relevant to determinand, image process unit
After (not being plotted in diagram) receives the pixel array, then carry out subsequent data processing.
Another preferred embodiment configuration of the invention as shown in Fig. 2, unlike more, photosensitive unit array 26 it is opposite
Allocation position is different, it is received be not determinand transmitted light 15, but the reflected light 25 of determinand, generation is relevant to
After the pixel array of determinand, similarly, subsequent data processing is carried out by image process unit.
Above-mentioned photosensitive unit array can be photosensitive coupling element (also known as charge coupled cell, Charge-coupled
Device is abbreviated as CCD) or complementary metal oxide semiconductor (being abbreviated as CMOS) Image Sensor, both elements are all collection
At circuit, current integrated circuit technology not only has reliable yield, but also visits integrated circuit technology progress and grant, these yuan
What the photosurface of each photosensitive unit can be produced in part is extremely tiny.
Since the array that tiny photosensitive element is arranged in is enough to serve as reasons the region cutting that a small amount of determinand is covered
The array imaging that pixel is constituted is to carry out subsequent screening and image processing, therefore, using photosensitive unit array as life
The error of testing result can be effectively reduced by changing the optical electrical conversion element in detection device.
For technique now, whether CCD or CMOS Image Sensor, each photosensitive unit at least can
Electronic signal is generated according to the absorption intensity of red, green, blue simultaneously, therefore every in the pixel array of photosensitive unit array generation
The light that one pixel is absorbed can all be represented as Intensityx,y=(r, g, b)x,y, wherein subscript X, Y is the pixel
Column locations of the corresponding photosensitive unit in photosensitive unit array entirety, r, g, b are respectively that the absorption of red, green, blue is strong
Degree.But it is noted that current technology is difficult to only absorb the light of single wavelength, therefore narrowly, the absorption intensity of feux rouges
Referring to the absorption intensity in this spectral range of central wavelength 640+/- △ λ nm, the absorption intensity of green light and blue light is also same, in
Cardiac wave it is long with △ λ then depending on the color filter technology of photosensitive unit array.
Therefore, photosensitive unit array can be indicated the image of sample slot in the form of pixel array, with the picture of a 3x 3
For pixel array:
(r11,g11,b11)11(r12,g12,b12)12(r13,g13,b13)13
Image3x3=(r21,g21,b21)21(r22,g22,b22)22(r23,g23,b23)23
(r31,g31,b31)31(r32,g32,b32)32(r33,g33,b33)33
After pixel array is sent to image process unit, each (r, g, b)x,yIt can be mapped in a color space,
Referring to figure 3., by taking common rgb color space as an example, wherein R axis represents red light intensity, G axis represents green intensity, B axle represents
Blue light strength, in color science, (0,0,0) represents black, (0,0,1) represents blue, (1,0,0) represents red, (0,1,0) generation
Table green, (1,1,0) represents yellow, (1,0,1) represents magenta, (0,1,1) represents cyan, and (1,1,1) represents white.Just
For the pixel array of aforementioned 3x 3, it can will at most be indicated in the form of 9 points in rgb color space.And according to photosensitive
The resolution of each photosensitive unit in cell array, the rgb color space of practical application and it is non-limiting be only capable of it is former using 1 as distance
The maximum distance of point, by taking 8 bin resolutions as an example, the eight power that the maximum distance apart from origin is two, i.e., 256, it parses herein
(0,0,0) represents black under degree, (0,0,256) represents blue, (256,0,0) represent red ..., and the rest may be inferred for remaining.But it must note
Meaning, is only a preferred embodiment of the invention using rgb color space, also using CIELUV color space or CIE
1964U*V*W* color space realizes similar effect.
When determinand total amount rareness, a feasible detection tricks of the trade are to dilute the determinand, so that it is met detection necessary
Minimum volume, to be detected, obtain another mistake after data derive dilute before the physical property that should have of determinand, still, object
Linear relationship is not always presented in the characteristics such as the optical property of matter and its concentration, reaction efficiency, and backstepping leads resulting result just at this time
There is error.
Referring to FIG. 4, this is the typical insufficient situation of determinand volume, determinand 43 is not enough to fill up sample slot 42, by
The electronic signal caused by photodiode is produced by the overall light intensity based on irradiation photosurface 41, it is clear that determinand volume
Electronic signal and real conditions have significant drop when insufficient.
Referring to FIG. 5, since photosensitive unit array 51 is constituted via multiple photosensitive units 52, it in the present embodiment, can
To generate the pixel array of a 5x 5, after image process unit receives the pixel array, which can be converted to of the same colour
Color space coordinate value array, and each 52 received total light intensity degree of institute of photosensitive unit is read, then according to each photosensitive unit 52
The received total light intensity degree of institute generates a preset color space coordinate threshold value with statistical, and with the preset color space coordinate
Threshold value excludes not by determinand overlay area color space coordinates value obtained or exclusive segment by determinand overlay area C
Color space coordinates value obtained, or even exclude determinand and cover insufficient region B color space coordinates value obtained, only
The region A sufficiently covered with determinand carries out subsequent detection to improve accuracy.
In another embodiment of the invention, preset color space coordinate threshold value can use the enclosure space in color space
It realizes, carries out subsequent detection to filter out color space coordinates value in certain color gamut.This preset color space coordinate
Threshold value setting means will be helpful to that the pixel generated by these particles is discharged there may be when precipitating particle in determinand,
And then improve accuracy in detection.
Another embodiment of the present invention, in mass detection, it is certain can with determinand occur chemical change cause it is to be measured
The reagent of object color change can be coated on sample rooved face in advance, after determinand injection, according to determinand color change
Degree determines certain physical property.
Referring to FIG. 6, be coated with the reagent of color X in sample slot 61, the determinand of color Y can with react and become
For peony, due to the effect of surface tension of liquid or cohesive force, only part determinand reaches the due thickness of examination criteria,
Such as region 65, the marginal portion of determinand then insufficient standard thickness, such as region 64, the color reaction in region 65 is only accurately
's.The present invention is produced in addition to can use aforementioned preset color space coordinate threshold value to obtain the photosensitive unit D of corresponding region 65
Raw color space coordinates value can also obtain color space coordinates value caused by photosensitive unit D in the way of color difference.
Color Y when reacting initial can be defined out a coordinate in color space, by taking rgb color space as an example,
It is expressed as (rY,gY,bY), and peony may be expressed as (rDR,gDR,bDR), then color difference △ C can be represented as (rDR-
rY,gDR-gY,bDR-bY), △ C can indicate that also can be converted is indicated with scale with vector form.
The color space coordinates value array at the end of the color space coordinates value array and reaction when reacting initial is obtained, i.e.,
The color space vector array of sample slot entirety can be obtained, △ C can be used as a threshold value to screen the color of the sample slot entirety
Space vector array can obtain effective color space vector array that photosensitive unit D or photosensitive unit F is generated.
Even if detection device starts to be also required to before detecting determinand measurement by school of being zeroed under the situation without reaction
Just, zero sample can also generate a color space coordinate value array, after the color space coordinates value array for obtaining determinand, pass through
The color sky for representing determinand photon absorbing intensity in each photosensitive unit range can be obtained in the two color space coordinates value arrays
Between vector array;In the case where needing situation to be reacted, by one section of preset reaction time, effective color of determinand can be obtained
Space vector array, effective color space vector array can be used for calculating between determinand and reagent in each photosensitive unit range
Reaction rate either determinand concentration.
When generally using optical mode detection material concentration, Beer-Lambert law can be all utilized, it may be assumed that
Wherein A: absorbance, I0: incident intensity, It: transmitted intensity, T: transmittance, K: absorption coefficient or molar absorbance
Coefficient, l: the optical path length of extinction material, c: the concentration (g/L or mol/L) of extinction material.
The concept of Beer-Lambert law is that light penetrates a solution with extinction material, transmitted intensity and extinction
Material concentration is directly proportional, and the path passed through with light is inversely proportional, therefore can be expressed as follows:
It=I0Kl extinction material concentration (2)
Since the light of single wavelength, I can not be absorbedtWith I0Intensity in the actually +/- △ λ nm of range of wavelengths lambda, light
Path length l can also make formula (2) to simplify by adjusting determinand thickness, therefore can also be represented simply as:
Extinction material concentration=K ' I(λ+/-△λ) (3)
Wherein, I(λ+/-△λ)The beam intensity ratio absorbed by extinction material, i.e. It/I0, K ' is 1/K under the situation of transmission,
It then need to be with factor (factor) row adjustment again under the situation of refraction.
In addition to it is aforementioned threshold value is generated based on color space vector array itself other than, the present invention provides in another embodiment
Another mode for generating threshold value.With reference to Fig. 7, photosensitive unit array 26 is received under the configuration of the reflected light 25 of determinand, volume
One photodiode 72 of outer setting, the photosignal generated when the photosurface 73 of the photodiode is by illumination are transmitted to image
Processing unit, with the difference of determinand, determinand reflected light also can be different from the relative intensity between transmitted light, therefore, when with
When zero sample is zeroed, which can be by photoelectric signal transformation at the scale of generation mass color intensity, and is multiplied by
The luminous intensity received with photosensitive unit array 26 that one coefficient makes matches, and becomes an auxiliary color space coordinate value scale,
Since the auxiliary color space coordinate value scale represents the colour generation characteristic of zero sample, sat using the auxiliary color space
Scale value scale is as a threshold value, to exclude in color space coordinates value array caused by sample slot not by determinand overlay area
Color space coordinates value, and realize only to surveying the purpose that is measured of object overlay area.
With reference to Fig. 8, further, the photodiode 72 in aforementioned embodiments can be with an auxiliary photosensitive unit array
81 substitutions, first obtain a primary color space coordinate value array, then match the photosensitive list of the auxiliary after being zeroed with the sample that is zeroed
The luminous intensity that element array 81 and photosensitive unit array 26 receive forms an auxiliary color space coordinate value array, can be completed two
Absorption intensity between photosensitive unit array is synchronous, and such configuration is not intended to generate different screening threshold values, but for reality
Now to the real-time measurement of determinand, what photosensitive unit array 26 measured image in sample slot 24 is in color change, the photosensitive list of the auxiliary
Element array 81 can then measure the increase or reduction of the corresponding testing concentration in color change in sample slot 24.Such implementation
Mode can be directed to same reaction, while and the reduction of real-time monitoring reactant and the increase of product, allow user can be with
Occurent reaction event is studied more fully hereinafter.
In the present invention, I0It can be obtained by light source direct irradiation photosensitive unit array to each photosensitive unit
Incident intensity, determinand photon absorbing intensity I in each photosensitive unit rangetThen can by aforementioned color space vector array or effectively
It is obtained in color space vector array, it is corresponding in photosensitive unit array range to calculate determinand via formula (3)
Testing concentration array or effective testing concentration array.
The following are the implementation processes that the present invention measures blood glucose in the way of color difference, and Fig. 9 (a) is the saturating of sample slot of the invention
Optical image is penetrated, wherein the sample slot has been coated with the reagent that color reaction occurs with blood glucose, therefore presents light yellow.Blood to be measured
Liquid obtains blood plasma and injects the sample slot as sample, as shown in Fig. 9 (b), sample is opened immediately after entering sample slot after being centrifuged
Raw color reaction is originated, sample slot center portion is deepened by light yellow, and image process unit was just noted down every 2 seconds by photosensitive
The image that cell array is captured, Fig. 9 (c) figure are then the sample slot images that reaction starts latter 20 seconds, can be sent out by Fig. 9 (c) figure
It is existing, it is influenced by active force between reagent and sample, the result of color reaction is simultaneously uneven, by screening, for effective image
Region carries out record and calculating of the time to color difference, result such as following table (one):
Time (second) | Red light intensity (R) | Green intensity (G) | Blue light strength (B) | Color difference (△ C) |
0 (reaction starts) | 157 | 162 | 118 | 0 |
+2 | 132 | 134 | 111 | 38.184 |
+4 | 98 | 95 | 101 | 90.879 |
+6 | 86 | 80 | 95 | 110.878 |
+8 | 77 | 72 | 91 | 123.406 |
+10 | 74 | 66 | 90 | 129.958 |
+12 | 68 | 61 | 87 | 138.141 |
+14 | 67 | 58 | 85 | 141.439 |
+16 | 63 | 54 | 84 | 147.160 |
+18 | 63 | 53 | 83 | 148.128 |
+20 | 61 | 53 | 81 | 149.887 |
In table (one), the record numerical value of red light intensity, green intensity and blue light strength is each in effective imagery zone
Average value of the photosensitive unit to each luminous intensity;In the 2nd column, since proper color reaction originates, there is not any color difference, and when
Between be calculated as 0 second, red light intensity approximation green intensity, but it is many to be all higher than blue light strength, it is clear that color reaction is relatively close when originating
Being similar to yellow is;In the 12nd column of table (one), i.e., color reaction starts 20 seconds latter, and color reaction can be considered stopping, comparison three
Kind coloured light intensity, color reaction at this time should be bluer;Color difference in table (one) is all for reaction starting, such as reacts
The calculation of 4 seconds color difference intensity is [(157-98) after starting2+(162-95)2+(118-101)2]1/2, then after taking decimal point
Third position rounds up, and obtains 4 seconds after reaction starting color difference intensity 90.879.
Aforesaid way is followed, respectively the sample of blood sugar concentration known to difference measure and color analysis, acquirement is different
The value of chromatism of the sample of blood sugar concentration specific time after reaction starting, then blood sugar concentration is carried out to value of chromatism to those samples
Blood sugar concentration can be obtained to the equation of value of chromatism relationship in linear regression analysis, and the equation can be applied to this measurement method, lead to
Cross the blood sugar concentration that value of chromatism calculates determinand.
According to the data of table (one), Figure 10 can be made, by that can determine that chromatic aberration comes into stable state in figure, verify
Color reaction can be considered that color reaction stops for 20 seconds really after starting, and through the invention as can be seen that color reaction about
The variation that main color is occurred at the 4th second, blue is changed by yellow, existing only detection white light intensity changes (i.e. total light intensity
Degree variation) detection device can not then provide color be variation quantitative information, be only capable of by user naked eyes judge;For spy
It is even more impossible to provide the quantitative information that color is variation for the detection device of fixation light (i.e. particular range of wavelengths).
As described above, only presently preferred embodiments of the present invention, the non-restriction for embodiment of the present invention is under this invention
The simple or equivalence changes of claim and patent specification disclosure, should belong to the scope of the present invention.
Claims (16)
1. a kind of biochemical detection device, characterized by comprising:
One light source generates a light;
One transparent sample slot accommodates a determinand and receives the light to generate a sample slot image;
One photosensitive unit array receives and converts the sample slot image as a pixel array;
One image process unit receives the pixel array, and the pixel array is converted to one first color space coordinates value battle array
Column, and the first color space coordinates value array is screened according to one first preset color space coordinate threshold value, to form one second
Color space coordinates value array;And
One auxiliary photodiode couples the image process unit to receive a transmitted light of the determinand in the sample slot, and
A photosignal is generated, which receives and convert the photosignal as an auxiliary color space coordinate value scale.
2. a kind of biochemical detection device, characterized by comprising:
One light source generates a light;
One transparent sample slot accommodates a determinand and receives the light to generate a sample slot image;
One photosensitive unit array receives and converts the sample slot image as a pixel array;
One image process unit receives the pixel array, and the pixel array is converted to one first color space coordinates value battle array
Column, and the first color space coordinates value array is screened according to one first preset color space coordinate threshold value, to form one second
Color space coordinates value array;And
One auxiliary photosensitive unit array, couples the image process unit to receive a transmitted light of the determinand in the sample slot,
And a primary color space coordinate value array is generated, which receives the primary color space coordinate value array, and
An auxiliary color space coordinate value array is generated according to the primary color space coordinate value array.
3. device as claimed in claim 1 or 2, wherein the image process unit is according to one first preset color spatial value
Array and the second color space coordinates value array obtain a color space vector array.
4. device as claimed in claim 1 or 2, wherein the image process unit obtains one according to the color space vector array
Testing concentration array.
5. device as claimed in claim 1 or 2, wherein the light source is a wideband radiant.
6. device as claimed in claim 1 or 2, wherein the light source is a white light source.
7. device as claimed in claim 1 or 2, wherein the sample slot image is formed by a transmitted light of the determinand.
8. device as claimed in claim 1 or 2, wherein the sample slot image is formed by a reflected light of the determinand.
9. a kind of biochemical detection device, characterized by comprising:
One light source can produce a light;
One transparent sample slot has a reagent, and accommodates a determinand and receive the light to generate one first sample slot shadow
Picture, the reagent make the determinand generate a color change after a specified time interval, to form one second sample slot image;
One photosensitive unit array receives and converts the first sample slot image as one first pixel array, and receives and convert this
Second sample slot image is one second pixel array;And
One image process unit receives first pixel array and second pixel array, which is converted to
Second pixel array is converted to one second color space coordinates value array, and root by one first color space coordinates value array
The first color space coordinates value array and the second color space coordinates value are screened according to one first preset color spatial value
Array, to form a third color space coordinate value array and one the 4th color space coordinates value array, further according to the third color
Color space coordinate value array and the 4th color space coordinates value array obtain a color space vector array.
10. device as claimed in claim 9, wherein the image process unit screens the color according to a preset color space vector
Color space vector array obtains one according to effective color space vector array to obtain an effective color space vector array
Effective testing concentration array.
11. device as claimed in claim 9, wherein the image process unit converts the color space vector array as a color
Space scale array, and the color space scale array is screened to obtain an effective color sky according to a preset color space scale
Between scale array, and an effective testing concentration array is obtained according to the effective color space scale array.
12. a kind of biochemical detection methods, characterized by comprising:
One first pixel array and one second pixel array are obtained via a photosensitive unit array;
Converting first pixel array and second pixel array becomes one first color space coordinates value array and one second color
Color space coordinate value array;
The first color space coordinates value array and second color space coordinates are screened according to a preset color spatial value
Value array becomes a third color space coordinate value array and one the 4th color space coordinates value array;And
A color space vector is obtained according to the third color space coordinate value array and the 4th color space coordinates value array
Array.
13. method as claimed in claim 12, wherein first pixel array is generated by a zero sample image, second picture
Pixel array is generated by a sample to be tested image.
14. method as claimed in claim 13, also comprising obtaining a concentration array according to the color space vector array.
15. method as claimed in claim 12, wherein first pixel array is generated by a sample to be tested image, generate this
After one pixel array, by a prefixed time interval, which is generated by the sample to be tested image.
16. method as claimed in claim 15, also comprising screening the color space vector according to a preset color space vector
Array obtains an effective concentration battle array according to effective color space vector array to obtain an effective color space vector array
Column.
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