CN106769891A - Biochemical detection device and method - Google Patents
Biochemical detection device and method Download PDFInfo
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- CN106769891A CN106769891A CN201510811942.2A CN201510811942A CN106769891A CN 106769891 A CN106769891 A CN 106769891A CN 201510811942 A CN201510811942 A CN 201510811942A CN 106769891 A CN106769891 A CN 106769891A
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Abstract
A kind of biochemical detection device and method, belong to detection means and method field.The method is included：One first pel array and one second pel array are obtained via a photosensitive unit array；Changing first pel array and second pel array turns into one first color space coordinates value array and one second color space coordinates value array；Screening the first color space coordinates value array and the second color space coordinates value array according to a preset color spatial value turns into a third color space coordinates value array and one the 4th color space coordinates value array；And a color space vector array is obtained according to the third color space coordinates value array and the 4th color space coordinates value array.The present invention can with remover in itself and detection process various error factors so that improve the biochemistry detection degree of accuracy.
Description
Technical field
The present invention is related to a kind of detection means, relates more specifically to a kind of biochemical detection device and method.
Background technology
Current biochemical detection device is used mostly optical detection mode, and the detection mode sends detection light using light source,
Arrive at determinand and produce reflected light or transmitted light, specific wavelength model is filtered out by bandpass filter (Band pass filter)
The light of (the usually +/- 10nm of predetermined wavelength) is enclosed, then e-mail is converted light signals into photodiode (Photodiode)
Number, and compare the electronic signal of the electronic signal of determinand and zero sample under the particular range of wavelengths light, according to both electricity
The difference of subsignal can convert and obtain the information of testing concentration.
Above-mentioned detection mode must accurately control the error factor that deviation is likely to cause on optical path, such as light source,
Error factor on each assembling parts in error factor, or device, can all make in the technique such as photodiode or bandpass filter
Into the error of testing result.
And, the electronic signal that photodiode is produced is to be radiated in its PN junction by light beam and produced, and is being surveyed
When measuring a small amount of determinand, if the determinand cannot be covered uniformly in the PN junctions, the electronic signal that photodiode is produced will
The concentration of determinand cannot be accurately reflected.
The content of the invention
To overcome the defect of prior art, the present invention to obtain determinand using photosensitive unit array and produce reflected light or transmission
Light, while the light information that each photosensitive unit is received is divided into different group's coloured light strength informations, such as with rgb light spectrum point
Group, maps to rgb color space, and the light information for being received just can be converted into color space coordinates value array, i.e., to be measured
The aerial image information of thing.
The present invention provides a kind of biochemical detection methods, comprising：One first pel array is obtained via a photosensitive unit array
And one second pel array；Changing first pel array and second pel array turns into one first color space coordinates value battle array
Row and one second color space coordinates value array；The first color space coordinates value is screened according to a preset color spatial value
Array and the second color space coordinates value array turn into a third color space coordinates value array and one the 4th color space is sat
Scale value array；And a color is obtained according to the third color space coordinates value array and the 4th color space coordinates value array
Space vector array.
It is preferred that first pel array is produced by a zero sample image, second pel array is by a sample to be tested
Image is produced.
It is preferred that also include obtaining a concentration array according to the color space vector array.
It is preferred that first pel array is produced by a sample to be tested image, after producing first pel array, by one
Prefixed time interval, second pel array is produced by the sample to be tested image.
It is preferred that also effective to obtain one comprising the color space vector array is screened according to a preset color space vector
Color space vector array, and a valid density array is obtained according to effective color space vector array.
In addition, the present invention provides a kind of biochemical detection device, comprising：One light source, produces a light；One transparent sample groove,
Accommodate a determinand and receive the light to produce a sample groove image；One photosensitive unit array, receives and changes the sample groove
Image is a pel array；And an image process unit, the pel array is received, and the pel array is converted to one first
Color space coordinates value array, and the first color space coordinates value battle array is screened according to one first preset color space coordinates threshold value
Row, to form one second color space coordinates value array.
It is preferred that the image process unit is according to one first preset color space coordinates value array and second color space
Coordinate value array obtains a color space vector array.
It is preferred that the image process unit obtains a testing concentration array according to the color space vector array.
It is preferred that the light source is a wideband radiant.
It is preferred that the light source is a white light source.
It is preferred that the sample groove image is formed by a transmitted light of the determinand.
It is preferred that the sample groove image is formed by a reflected light of the determinand.
It is preferred that also comprising couple the image process unit an auxiliary photodiode with receiving the sample groove this treat
A transmitted light of thing is surveyed, and produces a photosignal, it is a complementary colors that the image process unit is received and changes the photosignal
Color space coordinate value scale.
It is preferred that should also in the sample groove to receive comprising the auxiliary photosensitive unit array for coupling the image process unit
One transmitted light of determinand, and a primary color space coordinate value array is produced, the image process unit receives the initial color
Space coordinates value array, and produce one to aid in color space coordinate value array according to the primary color space coordinate value array.
Furthermore, the present invention provides a kind of biochemical detection device, comprising：One light source, can produce a light；One transparent sample
Groove, with a reagent, and accommodates a determinand and receives the light to produce the one first sample groove image, the reagent to treat this
Thing is surveyed in a color change is produced after a specified time interval, to form one second sample groove image；One photosensitive unit array, connects
It is one first pel array to receive and change the first sample groove image, and it is one second to receive and change the second sample groove image
Pel array；And an image process unit, first pel array and second pel array are received, by first pixel battle array
Row are converted to one first color space coordinates value array, and second pel array is converted into one second color space coordinates value battle array
Row, and the first color space coordinates value array and second color space are screened according to one first preset color spatial value
Coordinate value array, to form a third color space coordinates value array and one the 4th color space coordinates value array, further according to this
Third color space coordinates value array and the 4th color space coordinates value array obtain a color space vector array.
It is preferred that the image process unit screens the color space vector array to take according to a preset color space vector
An effective color space vector array is obtained, and an effective testing concentration battle array is obtained according to effective color space vector array
Row.
It is preferred that the image process unit changes the color space vector array arranged into a color space scalar matrix, and root
The color space scale array is screened according to a preset color space scale to obtain an effective color space scale array, and according to
Effective color space scale array obtains an effective testing concentration array.
Aerial image information by obtaining determinand of the invention, can accurately select wherein effective aerial image
Information, it is to avoid because detection light is by the way that after the determinand of non-uniform Distribution, photodiode photosurface absorbs uneven treating
The error surveyed thing transmitted light or reflected light and produce, can more be analyzed and processed via such optical image reduces because of technique and assembling
Caused error, and then accuracy is lifted, while improving the production yield and reduces cost of biochemical detection device.
Brief description of the drawings
Other features of the invention and technique effect with reference to the accompanying drawings, and are clearly appeared from implementation method, wherein：
Fig. 1 is the configuration schematic diagram of a preferred embodiment of the present invention；
Fig. 2 is the configuration schematic diagram of another preferred embodiment of the invention；
Fig. 3 is rgb color space schematic diagram；
Schematic diagram when Fig. 4 is not enough existing detection means determinand volume；
Fig. 5 is schematic diagram when determinand volume is not enough in the present invention；
Schematic diagram when Fig. 6 is determinand uneven thickness in the present invention；
Fig. 7 is the configuration schematic diagram of a preferred embodiment of the present invention with a photodiode；
Fig. 8 is the configuration schematic diagram of the preferred embodiment that the present invention has an auxiliary photosensitive unit array；
Fig. 9 (a) be image of the transparent sample groove of the present invention as acquired by photosensitive unit array, wherein the transparent sample groove
The coated blood sugar reagent to blood sugar generation color reaction；
Fig. 9 (b) is determinand full of above-mentioned transparent sample groove, image when color reaction is initial；
Fig. 9 (c) is color reaction into image during stable state；
Figure 10 is decay, the increase of aberration intensity and the relation in reaction time of rgb signal intensity when the present invention detects blood sugar
Figure.
Description of reference numerals：
11....... light source 42....... transparent samples groove
12....... incident light 43....... determinands
13....... coupons 51....... photosensitive units array
14....... transparent sample groove 52....... photosensitive units
15....... transmitted light 61....... samples groove
16....... photosensitive unit array 62....... photosensitive unit arrays
21....... light source 63........ photosensitive units
22....... incident light 64........ deficiency standard thickness region
23....... coupons 65........ reaches the region of standard thickness
24....... transparent sample groove 71........ transmitted lights
25....... reflected light 72........ photodiodes
26....... the photosurface of photosensitive unit array 73........ photodiodes
41....... the photosurface 81........ of photodiode aids in photosensitive unit array
A........ the photosensitive unit for fully covering
B........ the photosensitive unit of deficiency is covered
C........ the photosensitive unit that part covers
D........ the photosensitive unit for being covered by standard thickness determinand
F........ by the photosensitive unit of thickness deficiency determinand covering
Specific embodiment
The present invention provides a kind of biochemical detection device, its preferably implement one configuration as shown in figure 1,11 pairs of placements of light source
Determinand in transparent sample groove 14 launches an incident light 12, after the incident light 12 penetrates determinand, the transmitted light 15 of generation
Received and produced the pel array for being relevant to determinand, image process unit by the photosensitive unit array 16 of the lower section of coupons 13
After (not being plotted in diagram) receives the pel array, then carry out follow-up data processing.
Another preferred embodiment configuration of the invention as shown in Fig. 2 unlike more, photosensitive unit array 26 it is relative
Allocation position is different, and what it was received is not the transmitted light 15 of determinand, but the reflected light 25 of determinand, generation is relevant to
After the pel array of determinand, similarly, follow-up data processing is carried out by image process unit.
Above-mentioned photosensitive unit array can be photosensitive coupling element (also known as charge coupled cell, Charge-coupled
Device, is abbreviated as CCD), or complementary metal oxide semiconductor (being abbreviated as CMOS) Image Sensor, both elements are all collection
Into circuit, current integrated circuit technology not only has reliable yield, and visits integrated circuit technology progress and grant, these yuan
What the photosurface of each photosensitive unit can be produced in part is extremely tiny.
By the region cutting that the array that tiny photo-sensitive cell is arranged in is enough to be covered a small amount of determinand is served as reasons
The array imaging that pixel is constituted to carry out follow-up screening and image processing, therefore, by the use of photosensitive unit array as life
Changing the optical electrical conversion element in detection means can effectively reduce the error of testing result.
For technique now, whether CCD or CMOS Image Sensors, each photosensitive unit at least can
It is simultaneously according to the absorption intensity of red, green, blue generation electronic signal therefore every in the pel array of photosensitive unit array generation
The light that one pixel is absorbed can all be represented as Intensityx,y=(r, g, b)x,y, wherein, subscript X, Y is the pixel
Column locations of the corresponding photosensitive unit in photosensitive unit array entirety, the absorption that r, g, b are respectively red, green, blue is strong
Degree.But it is noted that current technology is difficult to only absorb the light of single wavelength, therefore narrowly, the absorption intensity of feux rouges
Refer to the absorption intensity in centre wavelength 640+/- △ λ nm this spectral range, the absorption intensity of green glow and blue light is also same, in
Cardiac wave it is long with △ λ color filter technologies then according to photosensitive unit array depending on.
Therefore, photosensitive unit array can represent the image of sample groove in the form of pel array, with the picture of a 3x 3
As a example by pixel array：
(r11,g11,b11)11(r12,g12,b12)12(r13,g13,b13)13
Image3x3=(r21,g21,b21)21(r22,g22,b22)22(r23,g23,b23)23
(r31,g31,b31)31(r32,g32,b32)32(r33,g33,b33)33
After pel array is sent to image process unit, each (r, g, b)x,yCan be mapped in a color space,
Fig. 3 is refer to, by taking common rgb color space as an example, wherein R axles represent red light intensity, G axles and represent green intensity, B axle representative
Blue light strength, in color science, (0,0,0) represents black, (0,0,1) and represents blue, (1,0,0) and represents red, (0,1,0) generation
Table green, (1,1,0) represent yellow, (1,0,1) and represent magenta, (0,1,1) and represents cyan, and (1,1,1) represents white.Just
As a example by the pel array of foregoing 3x 3, be able at most will be represented in the form of 9 points in rgb color space.And according to photosensitive
The resolution of each photosensitive unit in cell array, the rgb color space of practical application and it is non-limiting be only capable of it is former as distance using 1
The ultimate range of point, by taking 8 bin resolutions as an example, apart from the eight power that the ultimate range of origin is two, i.e., 256, parses herein
(0,0,0) represents black, (0,0,256) and represents blue, (256,0,0) and represent red under degree ..., and the rest may be inferred for remaining.But must note
Meaning, is only a preferred embodiment of the invention using rgb color space, also using CIELUV color spaces or CIE
1964U*V*W* color spaces realize similar effect.
When determinand total amount is rare, a feasible detection tricks of the trade are to dilute the determinand, it is met detection necessary
Minimum volume, to be detected, another mistake derives the determinand physical property to be had before dilution after obtaining data, but, thing
The characteristics such as the optical property of matter and its concentration, reaction efficiency are not always presented linear relationship, and now backstepping leads the result of gained just
There is error.
Fig. 4 is refer to, this is the not enough situation of typical determinand volume, determinand 43 is not enough to fill up sample groove 42, by
In the electronic signal produced by photodiode be based on irradiation photosurface 41 overall light intensity produced by, it is clear that determinand volume
Electronic signal has significant drop with real conditions when not enough.
Fig. 5 is refer to, because photosensitive unit array 51 is constituted via multiple photosensitive units 52, in the present embodiment, can
To produce the pel array of a 5x 5, after image process unit receives the pel array, the pel array can be converted to of the same colour
Color space coordinate value array, and the total light intensity degree that each photosensitive unit 52 is received is read, then according to each photosensitive unit 52
The total light intensity degree for being received produces a preset color space coordinates threshold value with statistical, and with the preset color space coordinates
Threshold value excludes the color space coordinates value not obtained by determinand overlay area, or exclusive segment is by determinand overlay area C
The color space coordinates value for being obtained, or even the color space coordinates value that the not enough region B of determinand covering is obtained is excluded, only
The region A fully covered with determinand carries out subsequent detection to improve accuracy.
In another embodiment of the invention, preset color space coordinates threshold value can use the closing space in color space
To realize, subsequent detection is carried out with the color space coordinates value filtered out in certain color gamut.This preset color space coordinates
Threshold value setting means will be helpful in determinand there may be during precipitation particle, discharge the pixel produced by these particles,
And then improve accuracy in detection.
Another embodiment of the present invention, in mass detection, some can cause to be measured with determinand generation chemical change
The reagent of thing color change can in advance be coated on sample rooved face, after determinand injects, according to determinand color change
Degree determines some physical property.
Refer to Fig. 6, be coated with the reagent of color X in sample groove 61, the determinand of color Y can with react and become
It is peony, due to the effect of surface tension of liquid or cohesive force, only part determinand reaches the due thickness of examination criteria,
Such as region 65, the marginal portion of determinand then not enough standard thickness, such as region 64, the color reaction in region 65 is only accurately
's.The present invention is produced except that can be obtained the photosensitive unit D of corresponding region 65 using foregoing preset color space coordinates threshold value
Raw color space coordinates value, can also obtain the color space coordinates value produced by photosensitive unit D using the mode of aberration.
Color Y when reacting initial can be defined out a coordinate in color space, by taking rgb color space as an example,
It is expressed as (rY,gY,bY), and peony may be expressed as (rDR,gDR,bDR), then aberration △ C can just be represented as (rDR-
rY,gDR-gY,bDR-bY), △ C can be represented with vector form, it is also possible to be converted into being represented with scale.
Color space coordinates value array when reacting initial and the color space coordinates value array at the end of reaction are obtained, i.e.,
The overall color space vector array of sample groove can be obtained, △ C can screen the overall color of the sample groove as a threshold value
Space vector array, just can obtain effective color space vector array that photosensitive unit D or photosensitive unit F is produced.
Even if under without the situation of reaction, detection means is also required to by school of being zeroed before starting detection determinand measurement
Just, zero sample can also produce a color space coordinate value array, after obtaining the color space coordinates value array of determinand, pass through
The two color space coordinates value arrays, you can the color for obtaining representing determinand photon absorbing intensity in each photosensitive unit scope is empty
Between vector array；Under the situation for needing reaction, by one section of default reaction time, you can obtain effective color of determinand
Space vector array, effective color space vector array can be used to calculate between determinand and reagent in each photosensitive unit scope
Reaction rate or determinand concentration.
During the general detection material concentration using optical mode, Beer-Lambert laws can be all utilized, i.e.,：
Wherein A：Absorbance, I0：Incident intensity, It：Transmitted intensity, T：Transmittance, K：Absorption coefficient or molar absorbance
Coefficient, l：The light path length of extinction material, c：The concentration (g/L or mol/L) of extinction material.
The concept of Beer-Lambert laws is that light penetrates the solution with extinction material, its transmitted intensity and extinction
Material concentration is directly proportional, and the path passed through with light is inversely proportional, therefore can be expressed as follows：
It=I0Kl extinction materials concentration (2)
Due to the light of single wavelength, I can not possibly be absorbedtWith I0Intensity in the actually +/- △ λ nm of range of wavelengths lambda, light
Path length l can also cause that formula (2) simplifies by adjusting determinand thickness, therefore can also be represented simply as：
Extinction material concentration=K ' I(λ+/-△λ) (3)
Wherein, I(λ+/-△λ)The beam intensity ratio absorbed by extinction material, i.e. It/I0, K ' is 1/K under the situation of transmission,
Then need to be with factor (factor) row adjustment again under the situation of refraction.
Except it is foregoing threshold value is produced based on color space vector array in itself in addition to, the present invention provide in another embodiment
Another mode for producing threshold value.With reference to Fig. 7, photosensitive unit array 26 is received under the configuration of the reflected light 25 of determinand, volume
One photodiode 72 of outer setting, the photosurface 73 of the photodiode is subject to the photosignal produced during illumination to be sent to image
Processing unit, with the difference of determinand, determinand reflected light also can be different from the relative intensity between transmitted light, therefore, when with
Zero sample is when being zeroed, and can be multiplied by photoelectric signal transformation into the scale of generation mass color intensity by the image process unit
The luminous intensity received with photosensitive unit array 26 that one coefficient makes is matched, and turns into an auxiliary color space coordinate value scale,
The colour generation characteristic of zero sample is represented due to the auxiliary color space coordinate value scale, therefore is sat using the auxiliary color space
Scale value scale as a threshold value, to exclude in the color space coordinates value array produced by sample groove not by determinand overlay area
Color space coordinates value, and realize only to surveying the purpose that thing overlay area is measured.
With reference to Fig. 8, further, the photodiode 72 in aforementioned embodiments can be with an auxiliary photosensitive unit array
81 substitute, and a primary color space coordinate value array are first obtained after being zeroed with the sample that is zeroed, then match the photosensitive list of the auxiliary
The luminous intensity that element array 81 is received with photosensitive unit array 26, forms one and aids in color space coordinate value array, you can complete two
Absorption intensity synchronization between photosensitive unit array, such configuration is not intended to produce different screening threshold values, but for reality
Now to the real-time measurement of determinand, photosensitive unit array 26 measure image in sample groove 24 in color change, the photosensitive list of the auxiliary
Element array 81 can then measure the increase or reduction of the corresponding testing concentration in color change in sample groove 24.Such implementation
Mode can be directed to same reaction, while and real-time monitoring reactant reduction and the increase of product, it is allowed to user can be with
Occurent reaction event is studied more fully hereinafter.
In the present invention, I0Can be obtained to each photosensitive unit by light source direct irradiation photosensitive unit array
Incident intensity, determinand photon absorbing intensity I in each photosensitive unit scopetThen can be by foregoing color space vector array or effective
Obtained in color space vector array, it is corresponding in photosensitive unit array range to calculate determinand via formula (3)
Testing concentration array or effective testing concentration array.
The implementation process of blood sugar is below measured using aberration mode for the present invention, Fig. 9 (a) is the saturating of sample groove of the invention
Optical image is penetrated, wherein the sample groove has been coated with the reagent that color reaction occurs with blood sugar, therefore presents light yellow.Blood to be measured
Liquid obtains blood plasma as sample to inject the sample groove after centrifugation, and shown in such as Fig. 9 (b), sample into opening after sample groove immediately
Raw color reaction is originated, sample groove middle body is deepened by light yellow, and image process unit was just noted down by photosensitive every 2 seconds
The image that cell array is captured, Fig. 9 (c) figures are then the sample groove images of 20 seconds after reaction starts, and can be sent out by Fig. 9 (c) figures
It is existing, influenceed with sample intermolecular forces by reagent, the result of color reaction is simultaneously uneven, by screening, for effective image
Region carries out record and calculating, its result such as following table () of the time to aberration：
| Time (second) | Red light intensity (R) | Green intensity (G) | Blue light strength (B) | Aberration (△ C) |
| 0 (reaction starts) | 157 | 162 | 118 | 0 |
| +2 | 132 | 134 | 111 | 38.184 |
| +4 | 98 | 95 | 101 | 90.879 |
| +6 | 86 | 80 | 95 | 110.878 |
| +8 | 77 | 72 | 91 | 123.406 |
| +10 | 74 | 66 | 90 | 129.958 |
| +12 | 68 | 61 | 87 | 138.141 |
| +14 | 67 | 58 | 85 | 141.439 |
| +16 | 63 | 54 | 84 | 147.160 |
| +18 | 63 | 53 | 83 | 148.128 |
| +20 | 61 | 53 | 81 | 149.887 |
In table (), the record numerical value of red light intensity, green intensity and blue light strength is each in effective imagery zone
Average value of the photosensitive unit to each luminous intensity；In being arranged the 2nd, due to proper color reaction starting, there is not any aberration, and when
Between be calculated as 0 second, the approximate green intensity of red light intensity, but all many higher than blue light strength, it is clear that be nearer during color reaction starting
Being similar to yellow is；In the 12nd row of table (), i.e., color reaction starts 20 seconds afterwards, and color reaction can be considered stopping, contrast three
Coloured light intensity is planted, color reaction now should be bluer；Aberration in table () for example reacts all for starting is reacted
The calculation of the aberration intensity of 4 seconds is [(157-98) after starting2+(162-95)2+(118-101)2]1/2, then after taking decimal point
3rd rounds up, and obtains the aberration intensity 90.879 of 4 seconds after reaction starting.
Aforesaid way is followed, the sample of blood sugar concentration known to difference is measured and color analysis respectively, obtain different
The value of chromatism of the sample of blood sugar concentration special time after starting is reacted, then blood sugar concentration is carried out to value of chromatism to those samples
Linear regression analysis, you can obtain blood sugar concentration and check colors the equation of difference relationship, the equation is that can be applied to this measuring method, is led to
Cross the blood sugar concentration that value of chromatism calculates determinand.
According to the data of table (one), Figure 10 can be made, by that can determine that chromatic aberration comes into stable state in figure, checking
Color reaction can be considered that color reaction stops for 20 seconds really after starting, and by the present invention as can be seen that color reaction about
There is the change that main color is at the 4th second, blueness is changed into by yellow, existing only detection white light intensity change (i.e. total light intensity
Degree change) detection means cannot then provide color be change quantitative information, be only capable of by user naked eyes judge；For spy
The detection means of fixation light (i.e. particular range of wavelengths) cannot more provide the quantitative information that color is change.
As described above, only presently preferred embodiments of the present invention, non-is the restriction of embodiment of the present invention, under this invention
The simple or equivalence changes of claim and patent specification disclosure, should belong to covering scope of the invention.
Claims (17)
1. a kind of biochemical detection device, it is characterised in that include：
One light source, produces a light；
One transparent sample groove, accommodates a determinand and receives the light to produce a sample groove image；
One photosensitive unit array, it is a pel array to receive and change the sample groove image；And
One image process unit, receives the pel array, and the pel array is converted into one first color space coordinates value battle array
Row, and the first color space coordinates value array is screened according to one first preset color space coordinates threshold value, to form one second
Color space coordinates value array.
2. device as claimed in claim 1, the wherein image process unit are according to one first preset color spatial value battle array
Row and the second color space coordinates value array obtain a color space vector array.
3. device as claimed in claim 1, the wherein image process unit obtain one and treat according to the color space vector array
Survey thing concentration array.
4. device as claimed in claim 1, the wherein light source are a wideband radiant.
5. device as claimed in claim 1, the wherein light source are a white light source.
6. device as claimed in claim 1, wherein sample groove image is formed by a transmitted light of the determinand.
7. device as claimed in claim 1, wherein sample groove image is formed by a reflected light of the determinand.
8. device as claimed in claim 7, also comprising the auxiliary photodiode for coupling the image process unit receiving
A transmitted light of the determinand in the sample groove, and produce a photosignal, the image process unit to receive and change the photoelectricity
Signal is an auxiliary color space coordinate value scale.
9. device as claimed in claim 7, also aids in photosensitive unit array to connect comprising couple the image process unit one
A transmitted light of the determinand in the sample groove is received, and produces a primary color space coordinate value array, the image process unit
The primary color space coordinate value array is received, and produces one to aid in color space according to the primary color space coordinate value array
Coordinate value array.
10. a kind of biochemical detection device, it is characterised in that include：
One light source, can produce a light；
One transparent sample groove, with a reagent, and accommodates a determinand and receives the light to produce one first sample groove shadow
Picture, the reagent makes the determinand in a color change is produced after a specified time interval, to form one second sample groove image；
One photosensitive unit array, it is one first pel array to receive and change the first sample groove image, and receives and change this
Second sample groove image is one second pel array；And
One image process unit, receives first pel array and second pel array, and first pel array is converted to
One first color space coordinates value array, one second color space coordinates value array, and root are converted to by second pel array
The first color space coordinates value array and the second color space coordinates value are screened according to one first preset color spatial value
Array, to form a third color space coordinates value array and one the 4th color space coordinates value array, further according to the 3rd color
Color space coordinate value array and the 4th color space coordinates value array obtain a color space vector array.
11. devices as claimed in claim 8, the wherein image process unit screen the color according to a preset color space vector
Color space vector array obtains one to obtain an effective color space vector array according to effective color space vector array
Effective testing concentration array.
It is a color that 12. devices as claimed in claim 8, the wherein image process unit change the color space vector array
Space scale array, and it is empty to obtain an effective color to screen the color space scale array according to a preset color space scale
Between scale array, and an effective testing concentration array is obtained according to the effective color space scale array.
13. a kind of biochemical detection methods, it is characterised in that include：
One first pel array and one second pel array are obtained via a photosensitive unit array；
Changing first pel array and second pel array turns into one first color space coordinates value array and one second color
Color space coordinate value array；
The first color space coordinates value array and second color space coordinates are screened according to a preset color spatial value
Value array turns into a third color space coordinates value array and one the 4th color space coordinates value array；And
One color space vector is obtained according to the third color space coordinates value array and the 4th color space coordinates value array
Array.
14. methods as claimed in claim 11, wherein first pel array are produced by a zero sample image, second picture
Pixel array is produced by a sample to be tested image.
15. methods as claimed in claim 12, also comprising according to the color space vector array one concentration array of acquirement.
16. methods as claimed in claim 11, wherein first pel array are produced by a sample to be tested image, produce this
After one pel array, by a prefixed time interval, second pel array is produced by the sample to be tested image.
17. methods as claimed in claim 14, it is also vectorial comprising the color space is screened according to a preset color space vector
Array obtains valid density battle array to obtain an effective color space vector array according to effective color space vector array
Row.
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