CN106769364B - A kind of phytoplankton sample embedding medium and its application - Google Patents
A kind of phytoplankton sample embedding medium and its application Download PDFInfo
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Abstract
The present invention relates to a kind of embedding medium and its applications, and in particular to a kind of phytoplankton sample embedding medium and its application.Phytoplankton sample embedding medium of the invention includes fixative, cereal syrup and diluent, the volume ratio of the sum of the fixative and fixative and diluent is fixative: the volume ratio of the sum of (fixative+diluent)=5~15:100, the cereal syrup and fixative and diluent is cereal syrup: (fixative+diluent)=100:50~150.Phytoplankton sample embedding medium of the invention will not only destroy Phytoplankton Cells, also have preferable optics permeability, Phytoplankton Cells information can be completely retained in optical microscopy, provide basis for the classification of accurate Morphological Identification.
Description
Technical field
The present invention relates to a kind of embedding medium and its applications, and in particular to a kind of phytoplankton sample embedding medium and its application.
Background technique
In recent years, with the extensive development of marine environmental monitoring vocational work, country is to marine environmental monitoring quality management
Increasingly pay attention to.For the technical capability and level for examining the detection of each marine monitoring mechanism marine phytoplankton, marine ecology is improved
Environmental monitoring data quality, and reinforce the business exchange of each marine monitoring mechanism, National Bureau of Oceanography has formulated " national ocean successively
3 years action plans of ECOLOGICAL ENVIRONMENTAL MONITORING quality management " (2015-2017), " national marine eco-environment monitoring matter in 2016
Amount guarantees programme of work " etc..It is required according to relevant document, marine eco-environment monitoring business needs to form controllable quality technology
Standard.
Currently, according to " marine monitoring specification " (the 7th part offshore pollution Ecological Investigation and biological monitoring GB17378.7-
2007), " standard of marine survey " (the 6th part marine organisms investigate GB-T 12763.6-2007) etc. requires, marine phytoplankton
The main method of monitoring is microscopy counting method, it is desirable that carries out Morphological Identification point to phytoplankton sample using optical microscopy
Analysis, this requires the phytoplankton prints for detection need to retain the raw informations such as chromatoplast.
Marine phytoplankton sample is because it is organic to be often used removal chromatoplast etc. vulnerable to reasons such as microorganism decompositions for organic matter
The store method of matter.Common permanent slide preparation method is to use 30%~95% second using ethyl alcohol gradually evaporation
Alcohol is gradually centrifuged frustule, and gummy mounting is used after frustule natural drying.Although this method can be with long-term preservation
Marine phytoplankton sample, but due to removing the crucial identification classification information such as marine phytoplankton chromatoplast, in marine environment
It is especially in marine biological monitoring business and is not suitable in monitoring business, be not also able to satisfy and prepare standard sample of photo for Marine Planktonic
The requirement of biological monitoring quality control.
It therefore, is the needs for meeting marine biological monitoring quality management requirement, chromatoplast etc. can be retained by needing to develop one kind
And it is suitable for sample preparation method of the phytoplankton than test.
According to retrieval, the semipermanent sample preparation skill of marine phytoplankton that the country has not published or applied at present
Art, and to biological sample prepare relevant patent of invention there are several types of:
1, a kind of store method of biological sample, Chu Qingzhu, leaf are peaceful etc., Guangdong Ocean University, application number:
201510790222.2 the applying date: 2015-11-17.The patent of invention is being applied in examination.Its main method is will be fresh
Biological sample formalin to be saved immersion fix 10~36h after, it is solidifying not less than 12% polyacrylamide using concentration
Glue is sealed up for safekeeping；Sample after sealing up for safekeeping can protect that color is fresh-keeping, and color and form remain unchanged within 2 years for a long time.
2, the interior label fixer and its preparation method and application of formalin-fixed biological sample, Li Xiaolin, Bu Xiuwu
Deng, the First Affiliated Hospital of Third Military Medical University of PLA, application number: 201210550890.4 applyings date: 2012-
12-18.The patent is authorized, classification number: C09J189/00 (2006.01) I C09J11/06 (2006.01) I C09J7/04
(2006.01)I.Disclosure of the invention interior label fixer of formalin-fixed biological sample and its preparation method and application,
The component and weight percent of interior label fixer are gelatin 1%~60%；Allicin 0.1%~10%；Glacial acetic acid 0.1%~
10%；Surplus is water, and for fixing interior label in formalin-fixed biological sample, text is clearly as before after saving for a long time,
Label is not easily to fall off.
3, small biological sample preparing process with transparant water soluble resin, Tang Anke, Tang Fahui etc., Chongqing Normal University, application
Number: 200610054494.7 applyings date: 2006-07-28.The patent haves no right to fail because of Unpaid Annual Fee.The invention is related to one kind
Small biological sample preparing process with transparant water soluble resin, raw materials used is polyethylene glycol, transparent Lauxite (liquid) and ice vinegar
Acid.Transparent Lauxite liquid and polyethylene glycol weight ratio are 1: 0.4~2.3, and aggregated reaction forms transparent water-soluble resin.Ice
The additional amount of acetic acid (content >=99.5) is the 5 of the transparent total amount of polyethylene glycol and the progress polymerization reaction formation of transparent Lauxite
~15%, mainly play coagulator, makes biological sample with resin prepared by above-mentioned technique, key step is divided into three steps:
A. the preparation of primer, the preparation of B. embedding biological sample, the face C. glue, the sample of this method preparation has toughness, not easy to crack, can prevent
Worm, it is mould proof, and it is able to maintain the original color of sample, it is a kind of production method of higher small-sized biological sample of success rate.
4, a kind of method of transparently sealing biological microscope slide sample, Koryo English, Shang Haizhong etc., application number:
200910059511.X the applying date: 2009-06-01.The patent haves no right to fail because of Unpaid Annual Fee.The inventive method is to carry glass
Upper 2-3 drop varnish is dripped on the biological sample of on piece, varnish of waiting is naturally auxiliary usually to cover lid in the case where free from admixture, bubble-free
Slide, gently squeeze room temperature under place a week or 40 DEG C drying box 6 hours, after capping agent, that is, varnish is solid, coverslip one
It holds labelled；With indicating sample title, specimen sampling, Production Time, producer's name.The invention biological microscope slide
Sample is carried out sealing light transmittance height up for safekeeping with varnish, is soluble in organic solvent, is not likely to produce bubble, to fanout free regions such as tissues, saves year
Limit for length, and it is quality-high and inexpensive, it is easy to draw materials；The sample produced is good with the transparency, durability is strong and fade-proof, never degenerates
It is saved with permanent, does not damage and destroy the technical effect for saving sample reset condition when taking.
5, a kind of glass slide Slide processing that phytoplankton microscope is taken pictures, Zhou Jian, Yang Guijun etc., application number:
201210309465.6 the applying date: 2012-08-28；Publication number: CN102902055A publication date: 2013-01-30.The invented party
Method is to take tradition 0.1ml/L phytoplankton production glass slide sample progress microscope to take pictures to be improved to 0.04ml/L, provides one
Microscope kind is simply and effectively improved to take pictures the method for image definition.
To sum up, at present published method be mainly used for being easy to fixed and cell inclusion can more complete preservation
Large and medium-sized biological sample or tissue sample, or the method for improving microscope clarity, being not directed to can be compared with
The fixed Slide processing for retaining marine phytoplankton cell characteristic for a long time, and semipermanent marine phytoplankton sample is because of it
The small, cell of individual is simple, once fixation is easily-deformable the problems such as, and the above method is not suitable for semipermanent marine phytoplankton
Sample preparation；Also, due also to the organic matters such as chromatoplast are vulnerable to microorganism decomposition, at present there is no can be used for semipermanent Marine Planktonic
The prior art of plant specimen preparation method.
Summary of the invention
A kind of phytoplankton sample embedding is provided it is an object of the invention to overcome above-mentioned the deficiencies in the prior art place
Agent and its application can completely retain the organic matters such as chromatoplast in phytoplankton using embedding medium preparation phytoplankton sample.
In addition, this method can be complete the present invention also provides the method using above-mentioned embedding medium preparation phytoplankton sample
Retain the organic matters such as chromatoplast in phytoplankton.
To achieve the above object, the technical scheme adopted by the invention is as follows: a kind of phytoplankton sample embedding medium comprising Gu
Determine agent, cereal syrup and diluent, the volume ratio of the sum of the fixative and fixative and diluent is fixative: (fixative+
Diluent)=5~15:100, the volume ratio of the sum of the cereal syrup and fixative and diluent is cereal syrup: (fixative
+ diluent)=100:50~150.
In phytoplankton sample embedding medium of the invention, the mobility of cereal syrup is moderate.Phytoplankton mark of the invention
This embedding medium will not only destroy Phytoplankton Cells, also have preferable optics permeability, can be complete in optical microscopy
Retain Phytoplankton Cells information, provides basis for the classification of accurate Morphological Identification.
Phytoplankton sample embedding medium of the present invention the preparation method comprises the following steps: first mix fixative and diluent, fixative is made
Solution, then cereal syrup is proportionally added into the solution of fixative, obtains embedding medium.Phytoplankton sample embedding of the present invention
In agent, the concentration of cereal syrup is not less than 40%.
As the preferred embodiment of phytoplankton sample embedding medium of the present invention, the fixative is fixed for aldehydes
Agent, the cereal syrup are malt syrup, and the diluent is water；It is highly preferred that the aldehyde fixative is glutaraldehyde, poly
At least one of formaldehyde.
In addition, the application the present invention also provides above-mentioned embedding medium in preparation phytoplankton sample.Above-mentioned embedding medium is used
In preparation phytoplankton sample, obtained phytoplankton sample can completely retain Phytoplankton Cells information.
Finally, the present invention also provides a kind of preparation methods of phytoplankton sample, in order to achieve this, the present invention takes
Technical solution are as follows: a kind of preparation method of phytoplankton sample, comprising the following steps:
(1) fixed: fixative A being added into phytoplankton sample, stands, obtains through fixed phytoplankton sample；
(2) it filters: through fixed phytoplankton sample obtained by filtration step (1), phytoplankton being filled on filter membrane；
(3) it embeds: the filter membrane obtained by step (2) with phytoplankton is put into embedding medium, and make floating on filter membrane
Trip plant is disengaged in embedding medium, takes out filter membrane, obtains the embedding medium containing phytoplankton；Wherein, the embedding medium includes solid
Determine agent B, cereal syrup and diluent, the volume ratio of the sum of fixative B and fixative B and diluent is fixative B:(fixative B
+ diluent)=5~15:100, the volume ratio of the sum of the cereal syrup and fixative B and diluent is cereal syrup: (fixed
Agent B+ diluent)=100:50~150；
(4) film-making: the embedding medium containing phytoplankton obtained by aspiration step (3) drops on glass slide, covered,
After embedding medium solidification, with resin mounting.
The preparation method of phytoplankton sample of the present invention, due to using specific embedding medium, which not only will not
Phytoplankton Cells are destroyed, also there is preferable optics permeability, the phytoplankton sample being obtained by this method utmostly is protected
Stayed marine phytoplankton cellular informatics, provide basis for the classification of accurate Morphological Identification, and have toughness, it is not easy to crack,
Energy insect prevention, mould proof etc..In addition, after using resin mounting, completely cut off air, phytoplankton sample obtained can the long period (extremely
It is 3 months few) it is preserved at room temperature.
The preferred embodiment of preparation method as phytoplankton sample of the present invention, the fixative B are aldehydes
Fixative, the cereal syrup are malt syrup, and the diluent is water.
The preferred embodiment of preparation method as phytoplankton sample of the present invention, the fixative A are aldehydes
Fixative.
The more preferable embodiment of preparation method as phytoplankton sample of the present invention, the fixative A are added to
After in phytoplankton sample, volumetric concentration of the fixative A in phytoplankton sample is 2~7%.
The preferred embodiment of preparation method as phytoplankton sample of the present invention, the aldehyde fixative are penta
At least one of dialdehyde, paraformaldehyde.
The preferred embodiment of preparation method as phytoplankton sample of the present invention, the aperture of the filter membrane are little
In 5 μm.The partial size of phytoplankter is generally higher than 5 μm, and 5 μm of filter membrane is not more than using aperture, can be by the plant of swimming of acquisition
Object is collected complete.
The preferred embodiment of preparation method as phytoplankton sample of the present invention, the glass slide are to count
Frame, the volume of the counting frame are 0.1ml.Select the counting frame that volume is 0.1ml as specimen slide, sample symbol obtained
Close the national technical standard requirement of phytoplankton sample quality control.
Compared with prior art, the invention has the benefit that the present invention provides a kind of phytoplankton sample embedding medium,
In the embedding medium, the mobility of cereal syrup is moderate.Phytoplankton sample embedding medium of the invention will not only destroy plant of swimming
Object cell also has preferable optics permeability, Phytoplankton Cells information can be completely retained in optical microscopy, is accurate
Morphological Identification classification provide basis.
The present invention also provides a kind of preparation methods of phytoplankton sample, and the process employs phytoplanktons of the invention
Sample embedding medium, the phytoplankton sample being obtained by this method utmostly remain marine phytoplankton cellular informatics, subject to
True Morphological Identification classification provides basis, and has toughness, not easy to crack, energy insect prevention, mould proof etc..In addition, this method uses
After resin mounting, air is completely cut off, phytoplankton sample obtained can be preserved at room temperature the long period (at least three moon).
The preparation method of phytoplankton sample of the present invention is particularly suitable for the quality management of halomereid monitoring business
Work.
Detailed description of the invention
Fig. 1 is the photo figure of phytoplankton sample after phytoplankton sample being made 2 months according to the method for the present invention；
Fig. 2 is the result figure under movable box-like algae (Biddulphia mobiliensis) 200 times of light microscopics；
Fig. 3 is the result figure revolved under chain Chaetoceros (Chaetoceros curvisetus) 200 times of light microscopics；
Fig. 4 is the result figure having under tail fin algae (Dinophysis caudata) 200 times of light microscopics；
Fig. 5 is the result figure under Killer Mincei (Karenia mikimotoi) 400 times of light microscopics；
Fig. 6 is the result figure under wavy Shi Sizao (Lithodesmium undulatum) 200 times of light microscopics；
Fig. 7 is the result figure under Pseudo nitzschia (Pseudo-nitzschia sp.) 200 times of light microscopics.
Specific embodiment
Purpose in order to better illustrate the present invention, technical scheme and beneficial effects, below in conjunction with attached drawing and specific implementation
The invention will be further described for example.
Embodiment 1
A kind of embodiment of phytoplankton sample embedding medium of the present invention, phytoplankton sample embedding medium described in the present embodiment by
The group of following parts by volume is grouped as: 5 parts of fixative, 200 parts of cereal syrup, 95 parts of diluent (that is: fixative and fixative and dilute
The volume ratio for releasing the sum of agent is fixative: (fixative+diluent)=5:100, cereal syrup and the sum of fixative and diluent
Volume ratio be cereal syrup: (fixative+diluent)=100:50)；Wherein, the fixative is glutaraldehyde, the cereal
Syrup is malt syrup, and the diluent is water.
Phytoplankton sample embedding medium described in the present embodiment the preparation method comprises the following steps: first fixative is dissolved in diluent in proportion
In, the aqueous solution of fixative is made, then cereal syrup is proportionally added into the aqueous solution of fixative, obtains phytoplankton sample
Embedding medium.
Embodiment 2
A kind of embodiment of phytoplankton sample embedding medium of the present invention, phytoplankton sample embedding medium described in the present embodiment by
The group of following parts by volume is grouped as: 15 parts of fixative, 100 parts of cereal syrup, 85 parts of diluent (that is: fixative and fixative and
The volume ratio of the sum of diluent is fixative: (fixative+diluent)=15:100, cereal syrup and fixative and diluent it
The volume ratio of sum is cereal syrup: (fixative+diluent)=100:100)；Wherein, the fixative is paraformaldehyde, described
Cereal syrup is malt syrup, and the diluent is water.
The preparation method is the same as that of Example 1 for phytoplankton sample embedding medium described in the present embodiment.
Embodiment 3
A kind of embodiment of phytoplankton sample embedding medium of the present invention, phytoplankton sample embedding medium described in the present embodiment by
Fixative, cereal syrup and diluent composition；The fixative be 10 parts by volume, diluent be 90 parts by volume (that is: fixative with
The volume ratio of the sum of fixative and diluent is fixative: (fixative+diluent)=10:100)；The cereal syrup and solid
The volume ratio for determining the sum of agent and diluent is cereal syrup: (fixative+diluent)=100:150；Wherein, the fixative is
Glutaraldehyde, the cereal syrup are malt syrup, and the diluent is water.
The preparation method is the same as that of Example 1 for phytoplankton sample embedding medium described in the present embodiment.
Embodiment 4
A kind of embodiment of the preparation method of phytoplankton sample of the present invention, the present embodiment are embedded using described in embodiment 1
Agent, phytoplankton sample described in the present embodiment the preparation method comprises the following steps:
(1) fixed: glutaraldehyde is added in the phytoplankton sample of the sea area Xiang Ziran acquisition, stands 10~20 minutes, obtains
Through fixed phytoplankton sample；Wherein, after glutaraldehyde adds in phytoplankton sample, glutaraldehyde is in phytoplankton sample
Volumetric concentration is 2%；
(2) it filters: through fixed phytoplankton sample obtained by filtration step (1), it is little that phytoplankton being filled into aperture
In on 5 μm of filter membranes (Millipore)；
(3) it embeds: embedding medium is poured into 4mL centrifuge tube, and the filter membrane obtained by step (2) with phytoplankton is put
Enter in centrifuge tube, shake filter membrane, be disengaged to the phytoplankton on filter membrane in embedding medium, take out filter membrane, obtains planting containing swimming
The embedding medium of object；
(4) film-making: the embedding medium containing phytoplankton obtained by 200 μ L liquid-transfering gun aspiration steps (3), dropping to volume is
On the phytoplankton counting frame of 0.1ml, covered, after embedding medium solidifies (about two days), with syringe receptive resin, edge
Coverslip edge drip resin, mounting.
Embodiment 5
A kind of embodiment of the preparation method of phytoplankton sample of the present invention, the present embodiment are embedded using described in embodiment 2
Agent, phytoplankton sample described in the present embodiment the preparation method comprises the following steps:
(1) fixed: paraformaldehyde is added in the phytoplankton sample of the sea area Xiang Ziran acquisition, stands 10~20 minutes, obtains
To through fixed phytoplankton sample；Wherein, after paraformaldehyde adds in phytoplankton sample, paraformaldehyde is in phytoplankton sample
Volumetric concentration in product is 5%；
(2) it filters: through fixed phytoplankton sample obtained by filtration step (1), it is little that phytoplankton being filled into aperture
In on 5 μm of filter membranes (Millipore)；
(3) it embeds: embedding medium is poured into 4mL centrifuge tube, and the filter membrane obtained by step (2) with phytoplankton is put
Enter in centrifuge tube, shake filter membrane, be disengaged to the phytoplankton on filter membrane in embedding medium, take out filter membrane, obtains planting containing swimming
The embedding medium of object；
(4) film-making: the embedding medium containing phytoplankton obtained by 200 μ L liquid-transfering gun aspiration steps (3), dropping to volume is
On the phytoplankton counting frame of 0.1ml, covered, after embedding medium solidifies (about two days), with syringe receptive resin, edge
Coverslip edge drip resin, mounting.
Embodiment 6
A kind of embodiment of the preparation method of phytoplankton sample of the present invention, the present embodiment are embedded using described in embodiment 3
Agent, phytoplankton sample described in the present embodiment the preparation method comprises the following steps:
(1) fixed: glutaraldehyde water solution is added in the phytoplankton sample of the sea area Xiang Ziran acquisition, stands 10~20 points
Clock is obtained through fixed phytoplankton sample；Wherein, after glutaraldehyde water solution adds in phytoplankton sample, glutaraldehyde is floating
Swimming the volumetric concentration in plant sample is 7%；
(2) it filters: through fixed phytoplankton sample obtained by filtration step (1), it is little that phytoplankton being filled into aperture
In on 5 μm of filter membranes (Millipore)；
(3) it embeds: embedding medium is poured into 4mL centrifuge tube, and the filter membrane obtained by step (2) with phytoplankton is put
Enter in centrifuge tube, shake filter membrane, be disengaged to the phytoplankton on filter membrane in embedding medium, take out filter membrane, obtains planting containing swimming
The embedding medium of object；
(4) film-making: the embedding medium containing phytoplankton obtained by 200 μ L liquid-transfering gun aspiration steps (3), dropping to volume is
On the phytoplankton counting frame of 0.1ml, covered, after embedding medium solidifies (about two days), with syringe receptive resin, edge
Coverslip edge drip resin, mounting.
Embodiment 7
We acquire marine phytoplankton sample, and prepare and mark according to the preparation method of phytoplankton sample of the present invention
Sample is made after 2 months in this, and the phytoplankton sample after embedding sealing is as shown in Figure 1.As seen from the figure, phytoplankton mark
This is after fixed sealing, and Phytoplankton Cells feature is clear, complete, and chromatoplast is complete, and cell is indeformable, generally preservation effect
Preferably.
Meanwhile we sample prepare 20 days after, in optical microphotograph microscopic observation marine phytoplankton sample, the knot of observation
Fruit is as shown in Figure 2 to 7.Wherein, Fig. 2 is the knot under movable box-like algae (Biddulphia mobiliensis) 200 times of light microscopics
Fruit figure；Fig. 3 is the result figure revolved under chain Chaetoceros (Chaetoceros curvisetus) 200 times of light microscopics；Fig. 4 is tool tail fin algae
Result figure under (Dinophysis caudata) 200 times of light microscopics；Fig. 5 is Killer Mincei (Karenia mikimotoi) 400
Result figure under times light microscopic；Fig. 6 is the result figure under wavy Shi Sizao (Lithodesmium undulatum) 200 times of light microscopics；
Fig. 7 is the result figure under Pseudo nitzschia (Pseudo-nitzschia sp.) 200 times of light microscopics.By Fig. 2~Fig. 7 as it can be seen that according to this
Marine phytoplankton sample made from inventive method, cell remain intact, and structure feature is obvious, and chromatoplast is significant.
Finally, it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than protects to the present invention
The limitation of range is protected, although the invention is described in detail with reference to the preferred embodiments, those skilled in the art should
Understand, it can be with modification or equivalent replacement of the technical solution of the present invention are made, without departing from the essence of technical solution of the present invention
And range.
Claims (10)
1. a kind of phytoplankton sample embedding medium, it is characterised in that: including fixative, cereal syrup and diluent, the fixation
The volume ratio of the sum of agent and fixative and diluent is fixative: (fixative+diluent)=5~15:100, the cerealose
The volume ratio of slurry and the sum of fixative and diluent is cereal syrup: (fixative+diluent)=100:50~150；It is described solid
Determining agent is aldehyde fixative.
2. phytoplankton sample embedding medium as described in claim 1, it is characterised in that: the cereal syrup is malt syrup,
The diluent is water.
3. phytoplankton sample embedding medium as described in claim 1, it is characterised in that: the aldehyde fixative be glutaraldehyde,
At least one of paraformaldehyde.
4. application of the embedding medium in preparation phytoplankton sample as described in claims 1 or 2 or 3.
5. a kind of preparation method of phytoplankton sample, it is characterised in that: the following steps are included:
(1) fixed: fixative A being added into phytoplankton sample, stands, obtains through fixed phytoplankton sample；It is described solid
Determining agent A is aldehyde fixative；
(2) it filters: through fixed phytoplankton sample obtained by filtration step (1), phytoplankton being filled on filter membrane；
(3) it embeds: the filter membrane obtained by step (2) with phytoplankton being put into embedding medium, and makes the plant of swimming on filter membrane
Object is disengaged in embedding medium, is taken out filter membrane, is obtained the embedding medium containing phytoplankton；Wherein, the embedding medium includes fixative
B, the volume ratio of cereal syrup and diluent, fixative B and the sum of fixative B and diluent is that fixative B:(fixative B+ is dilute
Release agent)=5~15:100, the volume ratio of the sum of the cereal syrup and fixative B and diluent is cereal syrup: (fixative B
+ diluent)=100:50~150；The fixative B is aldehyde fixative；
(4) film-making: the embedding medium containing phytoplankton obtained by aspiration step (3) drops on glass slide, covered, wait wrap
After burying agent solidification, with resin mounting.
6. the preparation method of phytoplankton sample as claimed in claim 5, it is characterised in that: the cereal syrup is maltose
Slurry, the diluent are water.
7. the preparation method of phytoplankton sample as claimed in claim 5, it is characterised in that: the fixative A, which is added to, to swim
After in plant sample, volumetric concentration of the fixative A in phytoplankton sample is 2~7%.
8. such as the preparation method of the described in any item phytoplankton samples of claim 5~7, it is characterised in that: the aldehydes is solid
Determining agent is at least one of glutaraldehyde, paraformaldehyde.
9. the preparation method of phytoplankton sample as claimed in claim 5, it is characterised in that: the aperture of the filter membrane is not more than
5μm。
10. the preparation method of phytoplankton sample as claimed in claim 5, it is characterised in that: the glass slide is counting frame,
The volume of the counting frame is 0.1ml.
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| CN104178475A (en) * | 2014-05-28 | 2014-12-03 | 华中农业大学 | Method for immobilizing microorganism bacteria |
| CN105039300A (en) * | 2015-08-19 | 2015-11-11 | 浙江工业大学 | Preparation method of heterogeneous bacteria embedding particles |
| CN106053126A (en) * | 2016-05-27 | 2016-10-26 | 中国水产科学研究院淡水渔业研究中心 | FFRC strain common carp mature ovarian tissue frozen section making method |
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