CN106769364A - A kind of phytoplankton sample embedding medium and its application - Google Patents
A kind of phytoplankton sample embedding medium and its application Download PDFInfo
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- CN106769364A CN106769364A CN201611113040.2A CN201611113040A CN106769364A CN 106769364 A CN106769364 A CN 106769364A CN 201611113040 A CN201611113040 A CN 201611113040A CN 106769364 A CN106769364 A CN 106769364A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/36—Embedding or analogous mounting of samples
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Abstract
The present invention relates to a kind of embedding medium and its application, and in particular to a kind of phytoplankton sample embedding medium and its application.Phytoplankton sample embedding medium of the invention includes fixative, cereal syrup and diluent, and the fixative is fixative with the volume ratio of fixative and diluent sum：(fixative+diluent)=5~15:100, the cereal syrup is cereal syrup with the volume ratio of fixative and diluent sum：(fixative+diluent)=100：50~150.Phytoplankton sample embedding medium of the invention will not only destroy Phytoplankton Cells, also with preferable optics permeability, Phytoplankton Cells information can completely be retained in light microscope, for the classification of accurate Morphological Identification provides basis.
Description
Technical field
The present invention relates to a kind of embedding medium and its application, and in particular to a kind of phytoplankton sample embedding medium and its application.
Background technology
In recent years, with the extensive development of marine environmental monitoring vocational work, country is to marine environmental monitoring quality management
Increasingly pay attention to.To check the technical capability and level of the marine phytoplankton detection of each marine monitoring mechanism, marine ecology is improved
Environmental monitoring data quality, and strengthen the business exchange of each marine monitoring mechanism, National Bureau of Oceanography formulates successively《National ocean
3 years action plans of ECOLOGICAL ENVIRONMENTAL MONITORING quality management》(2015-2017),《National marine eco-environment monitoring matter in 2016
Amount ensures programme of work》Deng.Required according to relevant document, marine eco-environment monitoring business is needed badly to form controllable quality technology
Standard.
Currently, according to《Marine monitoring specification》(the 7th part offshore pollution Ecological Investigation and biological monitoring GB 17378.7-
2007)、《Standard of marine survey》(the 6th part marine organisms investigate GB-T 12763.6-2007) etc. requires, marine phytoplankton
The main method of monitoring is microscopy counting method, it is desirable to Morphological Identification point is carried out to phytoplankton sample using light microscope
Analysis, this has required that for the phytoplankton print for detecting the raw informations such as chromatoplast need to be retained.
Marine phytoplankton sample is often organic using removal chromatoplast etc. because organic matter is easily by reasons such as microorganism decomposition
The store method of matter.Conventional permanent slide preparation method is to use ethanol progressively evaporation, i.e., using 30%~95% second
Alcohol is progressively centrifuged to frustule, and gummy mounting is used after frustule is spontaneously dried.Although the method can be preserved for a long time
Marine phytoplankton sample, but due to by the crucial identification classification information removal such as marine phytoplankton chromatoplast, in marine environment
It is particularly in marine organisms monitoring business in monitoring business and is not applied to, can not be also met and prepare standard sample of photo for Marine Planktonic
The requirement of biological monitoring quality control.
Therefore, the need for meet marine organisms monitoring quality management requirement, needing exploitation one kind badly can retain chromatoplast etc.
And the sample preparation method suitable for phytoplankton than test.
According to retrieval, not disclosed issue domestic at present or the semipermanent sample of marine phytoplankton applied prepare skill
Art, and preparing related patent of invention to biological sample has following several：
1st, a kind of store method of biological sample, it is peaceful etc. just to celebrate post, leaf, Guangdong Ocean University, application number：
201510790222.2, the applying date：2015-11-17.During the patent of invention applies examining.Its main method is will be fresh
Biological specimen formalin to be saved immersion fix 10~36h after, concentration be not less than 12% polyacrylamide coagulate
Glue is sealed up for safekeeping；It is fresh-keeping that sample after sealing up for safekeeping can for a long time protect color, and color and form keep constant within 2 years.
2nd, interior label fixer of formalin-fixed biological sample and its preparation method and application, Li Xiaolin, Bu Xiuwu
Deng the First Affiliated Hospital of Third Military Medical University of PLA, application number：201210550890.4 applyings date：2012-
12-18.The patent is authorized, classification number：C09J189/00(2006.01)I C09J11/06(2006.01)I C09J7/04
(2006.01)I.Disclosure of the invention interior label fixer of formalin-fixed biological sample and its preparation method and application,
The component and percentage by weight of interior label fixer are gelatin 1%~60%；Allicin 0.1%~10%；Glacial acetic acid 0.1%~
10%；Balance of water, for fixing interior label in formalin-fixed biological sample, word is clear as before after preserving for a long time,
Label is difficult for drop-off.
3rd, small biological sample preparing process with transparant water soluble resin, Tang Anke, Tang Fahui etc., Chongqing Normal University, application
Number：200610054494.7 applyings date：2006-07-28.The patent haves no right failure because of Unpaid Annual Fee.The invention is related to one kind
Small biological sample preparing process with transparant water soluble resin, raw materials used is polyethylene glycol, transparent Lauxite (liquid) and ice vinegar
Acid.Transparent Lauxite liquid is 1: 0.4~2.3 with polyethylene glycol weight ratio, and aggregated reaction forms transparent water-soluble resin.Ice
The addition of acetic acid (content >=99.5) is the 5 of the transparent total amount that polyethylene glycol carries out polymerisation formation with transparent Lauxite
~15%, mainly play coagulator, the resin prepared with above-mentioned technique makes biological sample, and key step is divided into three steps:
A. the preparation of primer, B. embeddings biological sample, the preparation of C. faces glue, sample tool toughness prepared by the method is not easy to crack, can prevent
Worm, it is mould proof, and the original color of sample can be kept, it is a kind of preparation method of success rate small-sized biological sample higher.
4th, a kind of method of transparently sealing biological microscope slide sample, Koryo English, Shang Haizhong etc., application number：
The 200910059511.X applyings date：2009-06-01.The patent haves no right failure because of Unpaid Annual Fee.The inventive method is to carry glass
Upper 2-3 drop varnish is dripped on biological sample on piece, varnish of waiting is naturally auxiliary usually to add a cover lid in the case of free from admixture, bubble-free
Slide, gently extrudes and place under normal temperature a week or 40 DEG C of drying boxes 6 hours, capping agent be varnish it is solid after, cover glass one
End is labelled；Indicate sample title, specimen sampling ground, Production Time, producer's name.The invention biological microscope slide
It is high that sample carries out sealing up for safekeeping light transmittance with varnish, is soluble in organic solvent, is not likely to produce bubble, to fanout free regions such as tissues, preserves year
Limit for length, and it is quality-high and inexpensive, it is easy to materials；The sample produced has that transparent good, durability is strong and fade-proof, never degenerates
With permanent preservation, the technique effect for preserving sample reset condition is not damaged and destroyed when taking.
5th, the slide Slide processing that a kind of phytoplankton microscope is taken pictures, Zhou Jian, Yang Guijun etc., application number：
201210309465.6 applyings date：2012-08-28；Publication number：CN102902055A publication date：2013-01-30.The invented party
Method is tradition is taken into 0.1ml/L phytoplanktons to make slide sample to carry out microscope and take pictures to be improved to 0.04ml/L, there is provided one
Kind simple and effective microscope that improves is taken pictures the method for image definition.
As fully visible, at present published method be mainly used in being easy to fixed and cell inclusion can more complete preservation
Big-and-middle-sized biological sample or tissue sample, or the method for improving microscope definition, being not directed to can be compared with
The Slide processing for retaining marine phytoplankton cell characteristic is fixed for a long time, and semipermanent marine phytoplankton sample is because of it
Individual small, cell is simple, the problems such as fixation is yielding, the above method is not suitable for semipermanent marine phytoplankton
It is prepared by sample；Also, due also to the organic matter such as chromatoplast is easily decomposed by microorganism, temporarily nothing can be used for semipermanent Marine Planktonic at present
In the prior art of plant specimen preparation method.
The content of the invention
A kind of phytoplankton sample is provided and is embedded it is an object of the invention to overcome above-mentioned the deficiencies in the prior art part
Agent and its application, preparing phytoplankton sample using the embedding medium can completely retain the organic matter such as chromatoplast in phytoplankton.
In addition, the method present invention also offers phytoplankton sample is prepared using above-mentioned embedding medium, the method can be complete
The organic matter such as chromatoplast in reservation phytoplankton.
To achieve the above object, the technical scheme taken of the present invention is：A kind of phytoplankton sample embedding medium, it includes solid
Determine agent, cereal syrup and diluent, the fixative is fixative with the volume ratio of fixative and diluent sum：(fixative+
Diluent)=5~15:100, the cereal syrup is cereal syrup with the volume ratio of fixative and diluent sum：(fixative
+ diluent)=100：50~150.
In phytoplankton sample embedding medium of the invention, the mobility of cereal syrup is moderate.Phytoplankton mark of the invention
This embedding medium will not only destroy Phytoplankton Cells, also with preferable optics permeability, can be complete in light microscope
Retain Phytoplankton Cells information, for the classification of accurate Morphological Identification provides basis.
The preparation method of phytoplankton sample embedding medium of the present invention is：First mix fixative and diluent, fixative is obtained
Solution, then cereal syrup is proportionally added into the solution of fixative, obtain embedding medium.Phytoplankton sample embedding of the present invention
In agent, the concentration of cereal syrup is not less than 40%.
Used as the preferred embodiment of phytoplankton sample embedding medium of the present invention, the fixative is fixed for aldehydes
Agent, the cereal syrup is malt syrup, and the diluent is water；It is highly preferred that the aldehyde fixative is glutaraldehyde, poly
At least one in formaldehyde.
In addition, the application present invention also offers above-mentioned embedding medium in phytoplankton sample is prepared.Above-mentioned embedding medium is used
In phytoplankton sample is prepared, the phytoplankton sample for obtaining can completely retain Phytoplankton Cells information.
Finally, present invention also offers a kind of preparation method of phytoplankton sample, in order to achieve this, the present invention takes
Technical scheme be：A kind of preparation method of phytoplankton sample, comprises the following steps：
(1) it is fixed：To fixative A is added in phytoplankton sample, stand, obtain through fixed phytoplankton sample；
(2) filter：Be filled into phytoplankton on filter membrane through fixed phytoplankton sample by filtration step (1) gained；
(3) embed：To be put into embedding medium by step (2) filter membrane of the gained with phytoplankton, and made floating on filter membrane
Plant is disengaged in embedding medium for trip, takes out filter membrane, obtains the embedding medium containing phytoplankton；Wherein, the embedding medium includes solid
Determine agent B, cereal syrup and diluent, the volume ratio of fixative B and fixative B and diluent sum is fixative B：(fixative B
+ diluent)=5~15:100, the cereal syrup is cereal syrup with the volume ratio of fixative and diluent sum：It is (fixed
Agent+diluent)=100：50~150；
(4) film-making：Aspiration step (3) embedding medium of the gained containing phytoplankton, drops on slide, covered,
After after embedding medium solidification, resin mounting is used.
The preparation method of phytoplankton sample of the present invention, as a result of specific embedding medium, the embedding medium not only will not
Destruction Phytoplankton Cells, also with preferable optics permeability, the phytoplankton sample being obtained by this method at utmost is protected
Stayed marine phytoplankton cellular informatics, for the classification of accurate Morphological Identification provides basis, and with toughness, it is not easy to crack,
Energy insect prevention, mould proof etc..Additionally, after using resin mounting, completely cut off air, obtained phytoplankton sample can the long period (extremely
It is few 3 months) preserved in normal temperature.
Used as the preferred embodiment of the preparation method of phytoplankton sample of the present invention, the fixative B is aldehydes
Fixative, the cereal syrup is malt syrup, and the diluent is water.
Used as the preferred embodiment of the preparation method of phytoplankton sample of the present invention, the fixative A is aldehydes
Fixative.
Used as the more preferably implementation method of the preparation method of phytoplankton sample of the present invention, the fixative A is added to
After in phytoplankton sample, volumetric concentrations of the fixative A in phytoplankton sample is 2~7%.
Used as the preferred embodiment of the preparation method of phytoplankton sample of the present invention, the aldehyde fixative is penta
At least one in dialdehyde, paraformaldehyde.
Used as the preferred embodiment of the preparation method of phytoplankton sample of the present invention, the aperture of the filter membrane is little
In 5 μm.The particle diameter of phytoplankter is generally higher than 5 μm, and 5 μm of filter membrane is not more than using aperture, the plant of swimming that will can be gathered
Thing is collected complete.
Used as the preferred embodiment of the preparation method of phytoplankton sample of the present invention, the slide is counting
Frame, the volume of the counting frame is 0.01ml/L.From the counting frame that volume is 0.01ml/L as specimen slide, system
The sample for obtaining meets the national technical standard requirement of phytoplankton sample quality control.
Compared with prior art, beneficial effects of the present invention are：The invention provides a kind of phytoplankton sample embedding medium,
In the embedding medium, the mobility of cereal syrup is moderate.Phytoplankton sample embedding medium of the invention will not only destroy plant of swimming
Thing cell, also with preferable optics permeability, can completely retain Phytoplankton Cells information in light microscope, be accurate
Morphological Identification classification provide basis.
Present invention also offers a kind of preparation method of phytoplankton sample, the process employs phytoplankton of the invention
Sample embedding medium, the phytoplankton sample being obtained by this method at utmost remains marine phytoplankton cellular informatics, is defined
True Morphological Identification classification provides basis, and with toughness, it is not easy to crack, can insect prevention, mould proof etc..Additionally, the method is used
After resin mounting, air is completely cut off, obtained phytoplankton sample can be preserved the long period (at least 3 months) in normal temperature.
The preparation method of phytoplankton sample of the present invention is particularly suited for the quality management that halomereid monitors business
Work.
Brief description of the drawings
Fig. 1 is phytoplankton sample to be obtained 2 months afterwards according to the inventive method, the photo figure of phytoplankton sample；
Fig. 2 is the result figure under movable box-like algae (Biddulphia mobiliensis) 200 times of light microscopics；
Fig. 3 is the result figure under rotation chain Chaetoceros (Chaetoceros curvisetus) 200 times of light microscopics；
Fig. 4 is the result figure under tool tail fin algae (Dinophysis caudata) 200 times of light microscopics；
Fig. 5 is the result figure under 400 times of light microscopics of the triumphant human relations algae of Michaelis (Karenia mikimotoi)；
Fig. 6 is the result figure under wavy stone silk algae (Lithodesmium undulatum) 200 times of light microscopics；
Fig. 7 is the result figure under 200 times of light microscopics of Pseudo nitzschia (Pseudo-nitzschia sp.).
Specific embodiment
For the purpose of the present invention, technical scheme and beneficial effect is better described, below in conjunction with accompanying drawing and specific implementation
The invention will be further described for example.
Embodiment 1
A kind of embodiment of phytoplankton sample embedding medium of the present invention, phytoplankton sample embedding medium described in the present embodiment by
The component composition of following parts by volume：5 parts of fixative, 200 parts of cereal syrup, 95 parts of diluent are (i.e.：Fixative and fixative and dilute
The volume ratio for releasing agent sum is fixative：(fixative+diluent)=5:100, cereal syrup and fixative and diluent sum
Volume ratio be cereal syrup：(fixative+diluent)=100:50)；Wherein, the fixative is glutaraldehyde, the cereal
Syrup is malt syrup, and the diluent is water.
The preparation method of phytoplankton sample embedding medium is described in the present embodiment：Fixative is first dissolved in diluent in proportion
In, the aqueous solution of fixative is obtained, then cereal syrup is proportionally added into the aqueous solution of fixative, obtain phytoplankton sample
Embedding medium.
Embodiment 2
A kind of embodiment of phytoplankton sample embedding medium of the present invention, phytoplankton sample embedding medium described in the present embodiment by
The component composition of following parts by volume：15 parts of fixative, 100 parts of cereal syrup, 85 parts of diluent are (i.e.：Fixative and fixative and
The volume ratio of diluent sum is fixative：(fixative+diluent)=15:100, cereal syrup and fixative and diluent it
The volume ratio of sum is cereal syrup：(fixative+diluent)=100:100)；Wherein, the fixative is paraformaldehyde, described
Cereal syrup is malt syrup, and the diluent is water.
The preparation method of phytoplankton sample embedding medium is with embodiment 1 described in the present embodiment.
Embodiment 3
A kind of embodiment of phytoplankton sample embedding medium of the present invention, phytoplankton sample embedding medium described in the present embodiment by
Fixative, cereal syrup and diluent composition；The fixative be 10 parts by volume, diluent be 90 parts by volume (i.e.：Fixative with
The volume ratio of fixative and diluent sum is fixative：(fixative+diluent)=10:100)；The cereal syrup with it is solid
The volume ratio for determining agent and diluent sum is cereal syrup：(fixative+diluent)=100:150；Wherein, the fixative is
Glutaraldehyde, the cereal syrup is malt syrup, and the diluent is water.
The preparation method of phytoplankton sample embedding medium is with embodiment 1 described in the present embodiment.
Embodiment 4
A kind of embodiment of the preparation method of phytoplankton sample of the present invention, the present embodiment is embedded using described in embodiment 1
Agent, the preparation method of phytoplankton sample is described in the present embodiment：
(1) it is fixed：To glutaraldehyde is added in the phytoplankton sample that natural marine site gathers, 10~20 minutes are stood, obtained
Through fixed phytoplankton sample；Wherein, after glutaraldehyde is added in phytoplankton sample, glutaraldehyde is in phytoplankton sample
Volumetric concentration is 2%；
(2) filter：Phytoplankton is filled into aperture little by filtration step (1) gained through fixed phytoplankton sample
In on 5 μm of filter membranes (Millipore)；
(3) embed：Embedding medium is poured into 4mL centrifuge tubes, and will be put by step (2) filter membrane of the gained with phytoplankton
Enter in centrifuge tube, shake filter membrane, the phytoplankton on filter membrane is disengaged in embedding medium, take out filter membrane, obtain containing plant of swimming
The embedding medium of thing；
(4) film-making：With 200 μ L liquid-transfering guns aspiration step (3) embedding mediums of the gained containing phytoplankton, dropping to volume is
On the phytoplankton counting frame of 0.01ml/L, covered, after after embedding medium solidification (about two days), is drawn with syringe and set
Fat, resin, mounting are dripped along cover glass edge.
Embodiment 5
A kind of embodiment of the preparation method of phytoplankton sample of the present invention, the present embodiment is embedded using described in embodiment 2
Agent, the preparation method of phytoplankton sample is described in the present embodiment：
(1) it is fixed：To paraformaldehyde is added in the phytoplankton sample that natural marine site gathers, 10~20 minutes are stood, obtained
To through fixed phytoplankton sample；Wherein, after paraformaldehyde is added in phytoplankton sample, paraformaldehyde is in phytoplankton sample
Volumetric concentration in product is 5%；
(2) filter：Phytoplankton is filled into aperture little by filtration step (1) gained through fixed phytoplankton sample
In on 5 μm of filter membranes (Millipore)；
(3) embed：Embedding medium is poured into 4mL centrifuge tubes, and will be put by step (2) filter membrane of the gained with phytoplankton
Enter in centrifuge tube, shake filter membrane, the phytoplankton on filter membrane is disengaged in embedding medium, take out filter membrane, obtain containing plant of swimming
The embedding medium of thing；
(4) film-making：With 200 μ L liquid-transfering guns aspiration step (3) embedding mediums of the gained containing phytoplankton, dropping to volume is
On the phytoplankton counting frame of 0.01ml/L, covered, after after embedding medium solidification (about two days), is drawn with syringe and set
Fat, resin, mounting are dripped along cover glass edge.
Embodiment 6
A kind of embodiment of the preparation method of phytoplankton sample of the present invention, the present embodiment is embedded using described in embodiment 3
Agent, the preparation method of phytoplankton sample is described in the present embodiment：
(1) it is fixed：To glutaraldehyde water solution is added in the phytoplankton sample that natural marine site gathers, 10~20 points are stood
Clock, obtains through fixed phytoplankton sample；Wherein, after glutaraldehyde water solution is added in phytoplankton sample, glutaraldehyde is floating
Volumetric concentration in trip plant sample is 7%；
(2) filter：Phytoplankton is filled into aperture little by filtration step (1) gained through fixed phytoplankton sample
In on 5 μm of filter membranes (Millipore)；
(3) embed：Embedding medium is poured into 4mL centrifuge tubes, and will be put by step (2) filter membrane of the gained with phytoplankton
Enter in centrifuge tube, shake filter membrane, the phytoplankton on filter membrane is disengaged in embedding medium, take out filter membrane, obtain containing plant of swimming
The embedding medium of thing；
(4) film-making：With 200 μ L liquid-transfering guns aspiration step (3) embedding mediums of the gained containing phytoplankton, dropping to volume is
On the phytoplankton counting frame of 0.01ml/L, covered, after after embedding medium solidification (about two days), is drawn with syringe and set
Fat, resin, mounting are dripped along cover glass edge.
Embodiment 7
We acquire marine phytoplankton sample, and according to the preparation method preparation mark of phytoplankton sample of the present invention
This, is obtained sample 2 months afterwards, and the phytoplankton sample after embedding sealing is as shown in Figure 1.As seen from the figure, phytoplankton mark
This is after fixed sealing, and Phytoplankton Cells feature is clear, complete, and completely, cell is indeformable, generally preservation effect for chromatoplast
Preferably.
Meanwhile, we sample prepare 20 days after, in optical microphotograph Microscopic observation marine phytoplankton sample, the knot of observation
Fruit is as shown in Figure 2 to 7.Wherein, Fig. 2 is the knot under movable box-like algae (Biddulphia mobiliensis) 200 times of light microscopics
Fruit is schemed；Fig. 3 is the result figure under rotation chain Chaetoceros (Chaetoceros curvisetus) 200 times of light microscopics；Fig. 4 is tool tail fin algae
Result figure under (Dinophysis caudata) 200 times of light microscopics；Fig. 5 is the triumphant human relations algae of Michaelis (Karenia mikimotoi) 400
Result figure under times light microscopic；Fig. 6 is the result figure under wavy stone silk algae (Lithodesmium undulatum) 200 times of light microscopics；
Fig. 7 is the result figure under 200 times of light microscopics of Pseudo nitzschia (Pseudo-nitzschia sp.).From Fig. 2~Fig. 7, according to this
Marine phytoplankton sample obtained in inventive method, cell is remained intact, and substantially, chromatoplast is notable for architectural feature.
It is last to should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention rather than the present invention is protected
The limitation of scope is protected, although being explained in detail to the present invention with reference to preferred embodiment, one of ordinary skill in the art should
Understand, technical scheme can be modified or equivalent, without deviating from the essence of technical solution of the present invention
And scope.
Claims (10)
1. a kind of phytoplankton sample embedding medium, it is characterised in that：Including fixative, cereal syrup and diluent, the fixation
Agent is fixative with the volume ratio of fixative and diluent sum：(fixative+diluent)=5~15:100, the cerealose
Slurry is cereal syrup with the volume ratio of fixative and diluent sum：(fixative+diluent)=100：50~150.
2. phytoplankton sample embedding medium as claimed in claim 1, it is characterised in that：The fixative is aldehyde fixative,
The cereal syrup is malt syrup, and the diluent is water；Preferably, the aldehyde fixative is glutaraldehyde, paraformaldehyde
In at least one.
3. application of the embedding medium as claimed in claim 1 or 2 in phytoplankton sample is prepared.
4. a kind of preparation method of phytoplankton sample, it is characterised in that：Comprise the following steps：
(1) it is fixed：To fixative A is added in phytoplankton sample, stand, obtain through fixed phytoplankton sample；
(2) filter：Be filled into phytoplankton on filter membrane through fixed phytoplankton sample by filtration step (1) gained；
(3) embed：To be put into embedding medium by step (2) filter membrane of the gained with phytoplankton, and make the plant of swimming on filter membrane
Thing is disengaged in embedding medium, takes out filter membrane, obtains the embedding medium containing phytoplankton；Wherein, the embedding medium includes fixative
The volume ratio of B, cereal syrup and diluent, fixative B and fixative B and diluent sum is fixative B：(fixative B+ is dilute
Release agent)=5~15:100, the cereal syrup is cereal syrup with the volume ratio of fixative and diluent sum：(fixative+
Diluent)=100：50~150；
(4) film-making：Aspiration step (3) embedding medium of the gained containing phytoplankton, drops on slide, covered, waits to wrap
After burying agent solidification, resin mounting is used.
5. the preparation method of phytoplankton sample as claimed in claim 4, it is characterised in that：The fixative B is solid for aldehydes
Determine agent, the cereal syrup is malt syrup, and the diluent is water.
6. the preparation method of phytoplankton sample as claimed in claim 4, it is characterised in that：The fixative A is solid for aldehydes
Determine agent.
7. the preparation method of phytoplankton sample as claimed in claim 6, it is characterised in that：The fixative A is added to and swum
After in plant sample, volumetric concentrations of the fixative A in phytoplankton sample is 2~7%.
8. the preparation method of the phytoplankton sample as described in any one of claim 5~7, it is characterised in that：The aldehydes is consolidated
It is at least one in glutaraldehyde, paraformaldehyde to determine agent.
9. the preparation method of phytoplankton sample as claimed in claim 4, it is characterised in that：The aperture of the filter membrane is not more than
5μm。
10. the preparation method of phytoplankton sample as claimed in claim 4, it is characterised in that：The slide is counting frame,
The volume of the counting frame is 0.01ml/L.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110108537A (en) * | 2018-02-01 | 2019-08-09 | 中国科学技术大学 | A kind of embedding medium and embedding method for biological tissue embedding |
| CN115406739A (en) * | 2022-07-22 | 2022-11-29 | 华南农业大学 | Preparation method of permanent mounting sheet for phytoplankton quantitative analysis sample |
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| CN106053126A (en) * | 2016-05-27 | 2016-10-26 | 中国水产科学研究院淡水渔业研究中心 | FFRC strain common carp mature ovarian tissue frozen section making method |
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| CN110108537A (en) * | 2018-02-01 | 2019-08-09 | 中国科学技术大学 | A kind of embedding medium and embedding method for biological tissue embedding |
| CN110108537B (en) * | 2018-02-01 | 2020-08-25 | 中国科学技术大学 | Embedding medium and embedding method for embedding biological tissue |
| CN115406739A (en) * | 2022-07-22 | 2022-11-29 | 华南农业大学 | Preparation method of permanent mounting sheet for phytoplankton quantitative analysis sample |
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