CN106769352A - A kind of fixture for Cell sheet glass dyeing - Google Patents
A kind of fixture for Cell sheet glass dyeing Download PDFInfo
- Publication number
- CN106769352A CN106769352A CN201611069970.2A CN201611069970A CN106769352A CN 106769352 A CN106769352 A CN 106769352A CN 201611069970 A CN201611069970 A CN 201611069970A CN 106769352 A CN106769352 A CN 106769352A
- Authority
- CN
- China
- Prior art keywords
- sheet glass
- cell sheet
- fluid injection
- fixture
- bearing part
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000005357 flat glass Substances 0.000 title claims abstract description 44
- 238000004043 dyeing Methods 0.000 title claims abstract description 28
- 238000002347 injection Methods 0.000 claims abstract description 57
- 239000007924 injection Substances 0.000 claims abstract description 57
- 239000012530 fluid Substances 0.000 claims abstract description 54
- 238000007789 sealing Methods 0.000 claims description 12
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 9
- 239000012128 staining reagent Substances 0.000 abstract description 3
- 238000007654 immersion Methods 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 239000003795 chemical substances by application Substances 0.000 description 12
- 238000005119 centrifugation Methods 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000000975 dye Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 238000005299 abrasion Methods 0.000 description 1
- 238000007605 air drying Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000009795 derivation Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000002360 explosive Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a kind of fixture for Cell sheet glass dyeing, including：Bearing part for carrying Cell sheet glass, located at bearing part top and for packed cell slide in the compressing member of bearing part；The compressing member is provided with fluid injection pond, apocenosis passage, the intake chute between fluid injection pond and apocenosis passage；The intake chute is inclined obliquely.The present invention quickly realizes staining reagent and is separated with cell, improves the production cycle, has saved the use of staining reagent, while cross pollution when also avoid a kind of lower reagent immersion cell.Simple structure of the invention, dependable performance.
Description
Technical field
The present invention relates to a kind of fixture.
Background technology
At present, the dyeing to Cell sheet glass mainly uses dip-dye formula, and main use is dried, dries, air-drying after the completion of dip-dye
Mode be dried treatment, not only efficiency is low, of long duration, and be easily damaged cell or change reagent validity, lead
Cause dyeing failure.
The content of the invention
Part, dyes it is an object of the invention to provide one kind for Cell sheet glass in order to overcome the above-mentioned deficiencies of the prior art
Fixture, be mainly used in dyeing after residual agent is separated with Cell sheet glass using centrifugal process.
The technical scheme is that：A kind of fixture for Cell sheet glass dyeing, including：For carrying Cell sheet glass
Bearing part, located at bearing part top and for packed cell slide in the compressing member of bearing part；The compressing member is provided with fluid injection pond,
Apocenosis passage, the intake chute between fluid injection pond and apocenosis passage；The intake chute is inclined obliquely.
Its further technical scheme is：The bearing part is rotated with compressing member and is connected.
Its further technical scheme is：The intake chute is 8 ° -20 ° with the angular range of horizontal plane.
Its further technical scheme is：The compressing member is additionally provided with the fluid injection groove connected with fluid injection pond；The fluid injection groove sets
In the periphery in fluid injection pond, its bottom surface inclines to fluid injection pond, and the bottom surface of fluid injection groove and the angle of horizontal plane are 15-45 °.
Its further technical scheme is：The upper surface of the bearing part is provided with the groove for accommodating Cell sheet glass.
Its further technical scheme is：The intake chute is 15 ° -45 ° with the angular range of groove horizontal direction.
Its further technical scheme is：The interface arrangment of mutual clamping is provided between the bearing part and compressing member.
Its further technical scheme is：The compressing member is provided with the card adapter plate for extending downwardly；Set on the inside of the card adapter plate
Flexible clamping boss；The edge of the bearing part is clamped in clamping boss.
Its further technical scheme is：The fixture for Cell sheet glass dyeing also includes being fixed on fluid injection pond periphery
Sealing ring；Bottom of the sealing ring located at fluid injection pond.
Its further technical scheme is：The bottom of the bearing part is additionally provided with fixed structure.
The present invention having the technical effect that compared with prior art：A kind of fixture for Cell sheet glass dyeing, by compressing
Be fixed Cell sheet glass in bearing part by part, and fluid injection pond is right against the location of cell on slide, will by external equipment
Reagent is instilled from fluid injection pond, so as to be dyeed to the cell on slide, after the completion of cell dyeing, by the centrifugation of centrifugation apparatus
Motion, residual agent is discharged from after intake chute derivation by apocenosis passage, so as to realize that cell is separated with residual agent, it is ensured that
Cross pollution will not occur during a kind of lower reagent immersion cell, intake chute is inclined obliquely, under the centrifugal speed of setting, cell
Can stay on the Cell sheet glass of fluid injection pond lower end, and reagent then can from the discharge of falling liquid bath, therefore, after dyeing can be quickly real
Existing residual agent is separated with cell, has saved the time of slide processed, reduces the damage to cell.Structure letter of the invention
It is single, dependable performance.
Further, intake chute and the angular range of horizontal plane are 8 ° -20 °, in the presence of centrifugation apparatus, residual agent energy
Smoothly flowed out from intake chute, meanwhile, cell is not easy to be pulled away under the inertia of residual agent.
Further, the periphery in fluid injection pond is additionally provided with fluid injection groove, to increase the injection area of liquid-injection equipment.
Further, Cell sheet glass is positioned by the groove on bearing part so that finally stay on Cell sheet glass
Cell position is located in fluid injection pond, is conducive to cell dyeing.
Further, the bottom in fluid injection pond is provided with sealing ring, when making Cell sheet glass, can both ensure the mixing in fluid injection pond
Liquid will not leak outside in the case of centrifugal motion from sealing ring, it is also possible to buffer thrust of the compressing member to Cell sheet glass.
The invention will be further described with specific embodiment below in conjunction with the accompanying drawings.
Brief description of the drawings
Fig. 1 is a kind of three-dimensional view of fixture for Cell sheet glass dyeing of the present invention.
Fig. 2 is the explosive view of Fig. 1.
Fig. 3 is the decomposition view of Fig. 1.
Reference
The Cell sheet glass of 10 fixture 1
The fixed structure of 21 groove 22
The compressing member of 2 bearing part 3
The apocenosis passage of 31 fluid injection pond 32
The fluid injection groove of 33 intake chute 34
The clamping boss of 35 card adapter plate 36
4 sealing rings
Specific embodiment
It is further to technical scheme with reference to schematic diagram in order to more fully understand technology contents of the invention
Introduce and illustrate, but be not limited to this.
A kind of fixture for Cell sheet glass dyeing, including：Bearing part for carrying Cell sheet glass, on bearing part
Just and for packed cell slide in the compressing member of bearing part, compressing member is provided with fluid injection pond, apocenosis passage, located at fluid injection pond and row
Intake chute between liquid passage, intake chute is inclined obliquely.
As shown in figure 1, a kind of fixture 10 for Cell sheet glass dyeing, including：Bearing part for carrying Cell sheet glass
2, located at the top of bearing part 2 and for packed cell slide in the compressing member 3 of bearing part 2, bearing part 2 is rotated with compressing member 3 and connected
Connect.
Compressing member 3 is provided with fluid injection pond 31, apocenosis passage 32, the intake chute between fluid injection pond 31 and apocenosis passage 32
33.Fluid injection pond 31 is right against the location of cell on slide, reagent is instilled from fluid injection pond 31 by external equipment, so that right
Cell on slide is dyeed.Staining reagent is concentrated mainly in fluid injection pond, both can guarantee that the Color of cell, can be subtracted again
The use of few reagent, improves the utilization rate of reagent.
As shown in Figure 2 and Figure 3, compressing member 3 is additionally provided with the fluid injection groove 34 connected with fluid injection pond 31, and fluid injection groove 34 is located at fluid injection
The periphery in pond 31, its bottom surface inclines to fluid injection pond 31, and the bottom surface of fluid injection groove 34 and the angle of horizontal plane are 15-45 °, increased note
Area during liquid equipment fluid injection.
Intake chute 33 is inclined obliquely, and it is 8 ° -20 ° with the angular range of horizontal plane, because residual agent is in centrifugal force
In the presence of outbound flow, cell can also flow under inertia along intake chute 33, but because intake chute 33 has an oblique angle, cell centrifugation one
After small segment distance, can be back in fluid injection groove 34 along intake chute 33.Residual agent in fluid injection pond 31 is under the influence of centrifugal force
Dished out by the gradient of special angle, realize that cell is separated with residual agent, through substantial amounts of it is demonstrated experimentally that intake chute is inclined upwardly
Angle is that 10 ° -15 ° of effect is best.Also, after the completion of cell dyeing, can quickly realize that cell is separated with residual agent, contract
Subtract the film-making cycle, improve production efficiency, reduce the damage to cell.
The upper surface of bearing part 2 is provided with the groove 21 for accommodating Cell sheet glass 1, by the groove 21 pairs on bearing part 2
Cell sheet glass 1 is positioned so that the cell position finally stayed on Cell sheet glass 1 is adapted to dyeing.Intake chute 33 and groove 21
The angular range of horizontal direction is 15 ° -45 °, because residual agent is outwards arranged under the influence of centrifugal force, and can lateral deviation backward
Certain position is moved, therefore, intake chute 33 is to set diagonally backward.
The interface arrangment of mutual clamping is provided between bearing part 2 and compressing member 3, compressing member 3 is provided with the clamping for extending downwardly
Plate 35, the inner side of card adapter plate 35 is provided with flexible clamping boss 36, and the edge of bearing part 2 is clamped in clamping boss 36.Apocenosis passage
32 are set straight down, and after bearing part 2 and the clamping of compressing member 3, apocenosis passage 32 is located at the side of bearing part.By interface arrangment
So that bearing part 2 is fixedly connected with compressing member 3, is produced when preventing cell from drying at a high speed, between bearing part 2 and compressing member 3 and rocked,
Play a part of fixed slide, it is to avoid abrasion of the sealing ring 4 to cell and diagnostic factro.Also, bearing part 2 and compressing member 3
Between fastening be separately convenient to.
Fixture 10 for Cell sheet glass dyeing also includes being fixed on the sealing ring 4 of the periphery of fluid injection pond 31, and sealing ring 4 is located at
The bottom in fluid injection pond 31, during 3 packed cell slide 1 of compressing member, sealing ring 4 can buffer thrust.
The bottom of bearing part 2 is additionally provided with fixed structure 22, and fixture 10 is fixed on centrifugation apparatus by fixed structure 22.
In the present embodiment, fixed structure is a fixed column.
When making Cell sheet glass, first fixture 10 is fixed on centrifugation apparatus, after Cell sheet glass 1 is installed into bearing part 2,
Clamping bearing part 2 and compressing member 3 so that be pressed on Cell sheet glass 1 on bearing part 2 by compressing member 3, will be thin using liquid-injection equipment
Born of the same parents, reagent mixed liquor are injected into the fluid injection pond 31 of bearing part 2, and now, mixed liquor is accommodated in fluid injection pond 31, due to fluid injection pond
31 lower end is provided with sealing ring 4, and mixed liquor will not leak.After centrifugation apparatus are opened, residual agent is past under the influence of centrifugal force
Outflow, cell is then stayed in fluid injection pond 31.
It is above-mentioned that technology contents of the invention are only further illustrated with embodiment, it is easier to understand in order to reader, but not
Represent embodiments of the present invention and be only limitted to this, any technology done according to the present invention extends or recreates, by of the invention
Protection.Protection scope of the present invention is defined by claims.
Claims (10)
1. it is a kind of for Cell sheet glass dyeing fixture, it is characterised in that including：Bearing part for carrying Cell sheet glass, if
In bearing part top and for packed cell slide in the compressing member of bearing part；The compressing member is provided with fluid injection pond, apocenosis passage,
Intake chute between fluid injection pond and apocenosis passage；The intake chute is inclined obliquely.
2. it is according to claim 1 for Cell sheet glass dyeing fixture, it is characterised in that the bearing part and compressing member
Rotate connection.
3. it is according to claim 1 for Cell sheet glass dyeing fixture, it is characterised in that the intake chute and horizontal plane
Angular range be 8 ° -20 °.
4. it is according to claim 1 for Cell sheet glass dyeing fixture, it is characterised in that the compressing member be additionally provided with
The fluid injection groove of fluid injection pond connection；The fluid injection groove is inclined located at the periphery in fluid injection pond, its bottom surface to fluid injection pond, the bottom surface of fluid injection groove
It it is 15-45 ° with the angle of horizontal plane.
5. it is according to claim 1 for Cell sheet glass dyeing fixture, it is characterised in that the upper surface of the bearing part
It is provided with the groove for accommodating Cell sheet glass.
6. the fixture for Cell sheet glass dyeing according to claim 5, it is characterised in that the intake chute and groove are horizontal
It it is 15 ° -45 ° to the angular range in direction.
7. it is according to claim 1 for Cell sheet glass dyeing fixture, it is characterised in that the bearing part and compressing member
Between be provided with the interface arrangment of mutual clamping.
8. it is according to claim 7 for Cell sheet glass dyeing fixture, it is characterised in that the compressing member is provided with downwards
The card adapter plate of extension；Flexible clamping boss is provided with the inside of the card adapter plate；The edge of the bearing part is clamped in clamping boss.
9. it is according to claim 1 for Cell sheet glass dyeing fixture, it is characterised in that it is described for Cell sheet glass dye
The fixture of color also includes being fixed on the sealing ring of fluid injection pond periphery；Bottom of the sealing ring located at fluid injection pond.
10. it is according to claim 1 for Cell sheet glass dyeing fixture, it is characterised in that the bottom of the bearing part
It is additionally provided with fixed structure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611069970.2A CN106769352A (en) | 2016-11-28 | 2016-11-28 | A kind of fixture for Cell sheet glass dyeing |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611069970.2A CN106769352A (en) | 2016-11-28 | 2016-11-28 | A kind of fixture for Cell sheet glass dyeing |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106769352A true CN106769352A (en) | 2017-05-31 |
Family
ID=58905192
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611069970.2A Pending CN106769352A (en) | 2016-11-28 | 2016-11-28 | A kind of fixture for Cell sheet glass dyeing |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106769352A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108220121A (en) * | 2017-12-28 | 2018-06-29 | 无锡准因生物科技有限公司 | A kind of cell purification chip fixture apparatus |
| CN110608936A (en) * | 2019-10-24 | 2019-12-24 | 广州江元医疗科技有限公司 | Multi-slide clamp |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201949887U (en) * | 2011-01-07 | 2011-08-31 | 天津市宝坻区人民医院 | Dyeing glass slide clamp |
| CN202393969U (en) * | 2011-12-31 | 2012-08-22 | 三明博峰生物科技有限公司 | Film producing clamp for liquid-based thin-layer cell |
| CN102768142A (en) * | 2012-07-19 | 2012-11-07 | 广州市达诚医疗技术有限公司 | Dyeing device |
| CN102768141A (en) * | 2012-07-19 | 2012-11-07 | 广州市达诚医疗技术有限公司 | Dyeing bin |
| CN103217326A (en) * | 2013-03-28 | 2013-07-24 | 广州安必平医药科技有限公司 | Sample reaction cabin and detection reagent adding method |
| CN203865197U (en) * | 2014-06-05 | 2014-10-08 | 北京恒利安科技发展有限公司 | Novel plastic bucket cover |
| CN104568557A (en) * | 2014-12-22 | 2015-04-29 | 珠海迪尔生物工程有限公司 | Clamp for fixing slide |
| CN205248363U (en) * | 2015-12-23 | 2016-05-18 | 天能电池集团有限公司 | Acid feeding kettle |
| CN205719705U (en) * | 2016-06-25 | 2016-11-23 | 南京健邦锦源医疗仪器有限公司 | A kind of Thinprep pap test film-making folder |
-
2016
- 2016-11-28 CN CN201611069970.2A patent/CN106769352A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201949887U (en) * | 2011-01-07 | 2011-08-31 | 天津市宝坻区人民医院 | Dyeing glass slide clamp |
| CN202393969U (en) * | 2011-12-31 | 2012-08-22 | 三明博峰生物科技有限公司 | Film producing clamp for liquid-based thin-layer cell |
| CN102768142A (en) * | 2012-07-19 | 2012-11-07 | 广州市达诚医疗技术有限公司 | Dyeing device |
| CN102768141A (en) * | 2012-07-19 | 2012-11-07 | 广州市达诚医疗技术有限公司 | Dyeing bin |
| CN103217326A (en) * | 2013-03-28 | 2013-07-24 | 广州安必平医药科技有限公司 | Sample reaction cabin and detection reagent adding method |
| CN203865197U (en) * | 2014-06-05 | 2014-10-08 | 北京恒利安科技发展有限公司 | Novel plastic bucket cover |
| CN104568557A (en) * | 2014-12-22 | 2015-04-29 | 珠海迪尔生物工程有限公司 | Clamp for fixing slide |
| CN205248363U (en) * | 2015-12-23 | 2016-05-18 | 天能电池集团有限公司 | Acid feeding kettle |
| CN205719705U (en) * | 2016-06-25 | 2016-11-23 | 南京健邦锦源医疗仪器有限公司 | A kind of Thinprep pap test film-making folder |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108220121A (en) * | 2017-12-28 | 2018-06-29 | 无锡准因生物科技有限公司 | A kind of cell purification chip fixture apparatus |
| CN110608936A (en) * | 2019-10-24 | 2019-12-24 | 广州江元医疗科技有限公司 | Multi-slide clamp |
| CN110608936B (en) * | 2019-10-24 | 2024-03-22 | 广州江元医疗科技有限公司 | Multi-slide clamp |
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| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170531 |
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| RJ01 | Rejection of invention patent application after publication |