CN1067587C - Glossy ganoderma spore activating method to produce physiological active substance - Google Patents
Glossy ganoderma spore activating method to produce physiological active substance Download PDFInfo
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- CN1067587C CN1067587C CN98122269A CN98122269A CN1067587C CN 1067587 C CN1067587 C CN 1067587C CN 98122269 A CN98122269 A CN 98122269A CN 98122269 A CN98122269 A CN 98122269A CN 1067587 C CN1067587 C CN 1067587C
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- Prior art keywords
- ganoderma spore
- sporoderm
- produces
- spore
- active substances
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- Expired - Lifetime
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- 241000222336 Ganoderma Species 0.000 title claims abstract description 72
- 238000000034 method Methods 0.000 title claims abstract description 24
- 239000013543 active substance Substances 0.000 title claims abstract description 14
- 230000003213 activating effect Effects 0.000 title claims abstract description 10
- 230000035784 germination Effects 0.000 claims abstract description 20
- 239000004480 active ingredient Substances 0.000 claims abstract description 6
- 230000001939 inductive effect Effects 0.000 claims abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 21
- 230000001413 cellular effect Effects 0.000 claims description 12
- 238000001035 drying Methods 0.000 claims description 9
- 238000007654 immersion Methods 0.000 claims description 9
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- 239000012531 culture fluid Substances 0.000 claims description 6
- 238000004108 freeze drying Methods 0.000 claims description 6
- 108010059892 Cellulase Proteins 0.000 claims description 5
- 102000012286 Chitinases Human genes 0.000 claims description 5
- 108010022172 Chitinases Proteins 0.000 claims description 5
- 229940106157 cellulase Drugs 0.000 claims description 5
- 238000012545 processing Methods 0.000 claims description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 4
- 239000012153 distilled water Substances 0.000 claims description 4
- 238000002347 injection Methods 0.000 claims description 4
- 239000007924 injection Substances 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 4
- 239000010409 thin film Substances 0.000 claims description 4
- 229960002685 biotin Drugs 0.000 claims description 3
- 235000020958 biotin Nutrition 0.000 claims description 3
- 239000011616 biotin Substances 0.000 claims description 3
- 230000015556 catabolic process Effects 0.000 claims description 3
- 238000006731 degradation reaction Methods 0.000 claims description 3
- 235000013399 edible fruits Nutrition 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 3
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 3
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 3
- 238000001291 vacuum drying Methods 0.000 claims description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 2
- 235000016709 nutrition Nutrition 0.000 claims description 2
- 238000002791 soaking Methods 0.000 claims description 2
- 239000000243 solution Substances 0.000 claims description 2
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 abstract description 4
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 abstract description 4
- 102000019197 Superoxide Dismutase Human genes 0.000 abstract description 4
- 108010012715 Superoxide dismutase Proteins 0.000 abstract description 4
- 239000002799 interferon inducing agent Substances 0.000 abstract description 3
- 108090000790 Enzymes Proteins 0.000 abstract description 2
- 102000004190 Enzymes Human genes 0.000 abstract description 2
- 229930003427 Vitamin E Natural products 0.000 abstract description 2
- 239000002253 acid Substances 0.000 abstract description 2
- 230000010261 cell growth Effects 0.000 abstract description 2
- 229940088598 enzyme Drugs 0.000 abstract description 2
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 abstract description 2
- 239000003102 growth factor Substances 0.000 abstract description 2
- 150000002596 lactones Chemical class 0.000 abstract description 2
- 229940046009 vitamin E Drugs 0.000 abstract description 2
- 235000019165 vitamin E Nutrition 0.000 abstract description 2
- 239000011709 vitamin E Substances 0.000 abstract description 2
- 230000036983 biotransformation Effects 0.000 abstract 2
- 229940032362 superoxide dismutase Drugs 0.000 abstract 2
- 230000004913 activation Effects 0.000 abstract 1
- 238000002386 leaching Methods 0.000 abstract 1
- 238000012216 screening Methods 0.000 description 9
- 238000009423 ventilation Methods 0.000 description 8
- 240000008397 Ganoderma lucidum Species 0.000 description 6
- 235000001637 Ganoderma lucidum Nutrition 0.000 description 6
- 239000000284 extract Substances 0.000 description 3
- 230000000284 resting effect Effects 0.000 description 3
- GXCLVBGFBYZDAG-UHFFFAOYSA-N N-[2-(1H-indol-3-yl)ethyl]-N-methylprop-2-en-1-amine Chemical compound CN(CCC1=CNC2=C1C=CC=C2)CC=C GXCLVBGFBYZDAG-UHFFFAOYSA-N 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000007605 air drying Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000010408 film Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000004763 spore germination Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a method for activating dormant ganoderma spores to generate physiological active substances in the germinating period by germinating biotransformation. Mature and plump ganoderma spores are processed by the steps, such as soak for inducing germination, germinating cultivation, spore wall treatment, dryness, or leaching, etc. The present invention can efficiency reserve physiological active ingredients, such as interferon inducers, ganoderma spore lactone A, ganoderma spore acid A, spore ether, natural vitamin E, super oxide dismutase (SOD), active glucoprotein, various active enzyme, cell growth factors, etc., which have active groups and are generated by activation in the process of germinating biotransformation.
Description
The present invention relates to a kind of Ganoderma spore that is in the resting state that activates by biological germinating and produce the method for biological active substances at sprouting period.
Ganoderma is launched out the spore that resembles the film shape in the growth and maturity phase from cap and is called Ganoderma spore powder.Offer record according to the side, Ganoderma spore has the whole hereditary active substance of Ganoderma.To the effect of human body immunity improving function and mechanism and to the wall-breaking method of Ganoderma spore, carried out a large amount of research about Ganoderma and Ganoderma spore powder both at home and abroad.Relevant Ganoderma spore produces the problem of biological active substanceies such as interferon inducer at sprouting period, do not see bibliographical information so far.
It is tough and tensile double-deck sporoderm that small Ganoderma spore has very, it is slower that Ganoderma spore is sprouted speed in the Nature, and germination rate is extremely low, under optimum conditions generally through 24 to 48 hours extended germ tubes of, mycelia can form branch after 72 hours, and germination rate has only 3-15%.
The purpose of this invention is to provide a kind of method, can significantly improve the Ganoderma spore germination rate, activate the Ganoderma spore that is in the resting state and produce physiologically active ingredient at sprouting period with active group by biological germinating.
Method of the present invention is the Ganoderma spore of screening mature and plump, through immersion induce accelerating germination, technique process such as the cultivation of sprouting, sporoderm processing, drying or lixiviate, effectively keep Ganoderma spore and activate a large amount of biological active substances that produces through the biological germinating process, concrete steps are as follows:
One, accelerating germination is induced in immersion: the Ganoderma spore of screening mature and plump, add clear water, distilled water, normal saline or impel the nutritional solution of Ganoderma spore fast-germination to soak and induce accelerating germination, as Cortex cocois radicis fruit caving water, the 1-5% malt extract, 0.5-25% Ganoderma sporophore or Ganoderma mycelium leachate, 0.1-5% biotin culture fluid, 0.1-3% potassium dihydrogen phosphate and magnesium sulfate culture fluid, can select wherein one or more for use, addition is 0.1-5 a times of Ganoderma spore weight, soak time is decided on water temperature, soaks 30 minutes to 8 hours in 20-43 ℃ of water temperature usually.
Two, the cultivation of sprouting: the Ganoderma spore in will soaking is pulled out, drips to remove excessive moisture, is positioned over then to carry out the spore cultivation of sprouting in the heat and moisture preserving ventilation incubator.The relative humidity of cultivating is generally 65-98%, and cultivation temperature is 18-48 ℃, and the sprouting period time is 30 minutes to 24 hours.The Ganoderma spore rate of sprouting reaches more than 95%.Its sporoderm of Ganoderma spore that is in sprouting period is obviously softening, its sporoderm-broken rate of favourable raising.
Three, sporoderm is handled: will be through the Ganoderma spore of biological germinating, chitinase, cellulase degradation through common commercial Application under low temperature environment make its sporoderm lose toughness and embrittlement, or adopt common industrial micronizing, roll or grind machinery and carry out the micronizing breaking cellular wall, roll breaking cellular wall, grind breaking cellular wall or super-voltage micro jet breaking cellular wall, sporoderm-broken rate can surpass 99%.
Four, drying or lixiviate: under cryogenic conditions, carry out dried, comprise and adopt common industrial lyophilization, vacuum drying or cold drying etc.), finished product moisture content is less than 4%.Lixiviate can adopt that common industry water is carried, alcohol extraction or thin film concentration process are extracted active ingredient and be mixed with extractum, injection or oral liquid.
The inventive method Ganoderma spore rate of sprouting reaches more than 95%.Experiencing thousands of experiments and a large amount of pharmaceutical research and chemical analyses shows, activate the Ganoderma spore that is in the resting state by biological germinating technology and produce biological active substances at sprouting period, through further sporoderm processing, drying or extracting technology operation effectively keep Ganoderma spore and activate the interferon inducer that produces through the biological germinating process, Ganoderma spore lactone A, Ganoderma spore acid A, spore ether, natural Vitamin E, superoxide dismutase (SOD), active glucoprotein, various active enzyme and cell growth factor etc. have the physiologically active ingredient of active gene.
Below in conjunction with embodiment the present invention is described in further detail.
Embodiment one:
1. the Ganoderma spore of screening mature and plump is 100 kilograms, adds 100 kilograms of 1% malt extracts, soaks and induces accelerating germination 6 hours, 20 ℃ of water temperatures.
2. will induce the Ganoderma spore of accelerating germination to pull out through immersion, and drip and to remove excessive moisture, and be positioned over then and carry out the spore cultivation of sprouting in the heat and moisture preserving ventilation incubator.The incubator relative humidity is 70%, and cultivation temperature is 20 ℃, and the sprouting period time is 12 hours.On inspection, the Ganoderma spore rate of sprouting reaches 96%, and sporoderm is obviously softening.
3. handle through supermicro mill, wall breaking rate of ganoderma lucidum spores reaches 99%.
4. place conventional Minton dryer dried, finished product moisture content is less than 3%.
Embodiment two
1. the Ganoderma spore of screening mature and plump is 100 kilograms, adds 45 kilograms in Cortex cocois radicis fruit caving water, and distilled water soaks for 100 kilograms induced accelerating germination 30 minutes, 35 ℃ of water temperatures.
2. will induce the Ganoderma spore drip-dry moisture content of accelerating germination through immersion, and be positioned over and carry out the spore cultivation of sprouting in the heat and moisture preserving ventilation incubator, incubator relative humidity 80%, 36 ℃ of temperature, the sprouting period time is 5 hours.Through electron microscopic examination, Ganoderma spore is in the state of sprouting, and germ tube basically forms, and sporoderm is obviously softening.
3. add 0.1 kilogram of 0.1 kilogram of chitinase, cellulase to the Ganoderma spore enzymolysis of biological germinating 2 hours, hydrolysis temperature 40-55 ℃, make tough and tensile double-deck sporoderm lose toughness and embrittlement.Or further through the super micron mill broken wall treatment, wall breaking rate of ganoderma lucidum spores surpasses 99%.
4. place normal freeze-drying device dried, finished product moisture content is less than 2.8%.
Embodiment three:
1. 100 kilograms of the Ganoderma spores of screening mature and plump add 5% Ganoderma sporophore leachate and soak for 150 kilograms and induced accelerating germination 4 hours, 30 ℃ of water temperatures.
2. will induce the Ganoderma spore drip-dry moisture content of accelerating germination through immersion, and be positioned over and carry out the spore cultivation of sprouting in the heat and moisture preserving ventilation incubator, incubator relative humidity 65%, 38 ℃ of temperature, the time is 5 hours.Through electron microscopic examination, Ganoderma spore is in the state of sprouting, but does not stretch out germ tube as yet, and sporoderm is obviously softening.
3. through super-voltage micro jet device broken wall treatment, wall breaking rate of ganoderma lucidum spores surpasses 99%.
4. place normal freeze-drying device dried, finished product moisture content is less than 3.2%.
Embodiment four:
1. the Ganoderma spore of screening mature and plump is 100 kilograms, adds 200 kilograms of 2% biotin culture fluid, soaks and induces accelerating germination 3 hours, 40 ℃ of water temperatures.
2. will induce the Ganoderma spore of accelerating germination to pull out through immersion, and drip and to remove excessive moisture, and be positioned over then and carry out the spore cultivation of sprouting in the heat and moisture preserving ventilation incubator.The incubator relative humidity is 90%, and temperature is 33 ℃, and the sprouting period time is 4 hours.On inspection, the Ganoderma spore rate of sprouting reaches 95%, and sporoderm is obviously softening.
3. add 0.08 kilogram of chitinase, 0.2 kilogram of cellulase, hydrolysis temperature 40-55 ℃, the Ganoderma spore of biological germinating was carried out enzymolysis 3 hours, make tough and tensile double-deck sporoderm lose toughness and embrittlement.Roll processing through chaser, wall breaking rate of ganoderma lucidum spores surpasses 99%
4. placed conventional low temperature forced air drying device dry 12 hours, finished product moisture content is less than 4%.
Embodiment five:
1. the Ganoderma spore of screening mature and plump is 100 kilograms, adds 100 kilograms of 0.1% potassium dihydrogen phosphate and magnesium sulfate culture fluid, soaks and induces accelerating germination 8 hours, 21 ℃ of water temperatures.
2. will induce the Ganoderma spore drip-dry moisture content of accelerating germination through immersion, and be positioned over and carry out the spore cultivation of sprouting in the heat and moisture preserving ventilation incubator, incubator relative humidity 85%, 42 ℃ of temperature, the time is 3 hours.Through electron microscopic examination, Ganoderma spore is in the state of sprouting, and germ tube has been seen formation, and sporoderm is obviously softening.
3. adopt the extracting of common industry water lifting manipulation, and then through the alcohol extracting method extracting, merge extract and handle through thin film concentration.
4. further be mixed with extractum, injection or oral liquid.
Embodiment six:
1. the Ganoderma spore of screening mature and plump is 100 kilograms, adds 50 kilograms of 50 kilograms of 5% Ganoderma mycelium leachates and 1% malt extracts, soaks and induces accelerating germination 7 hours, 25 ℃ of water temperatures.
2. will induce the Ganoderma spore drip-dry moisture content of accelerating germination through immersion, and be positioned over and carry out the spore cultivation of sprouting in the heat and moisture preserving ventilation incubator, incubator relative humidity 85%, 25 ℃ of temperature, the time is 12 hours.Through electron microscopic examination, Ganoderma spore is in the state of sprouting, but does not stretch out germ tube as yet, and sporoderm is obviously softening.
3. through super-voltage micro jet device broken wall treatment, wall breaking rate of ganoderma lucidum spores surpasses 99%.
4. place normal freeze-drying device dried, finished product moisture content is less than 2.8%.
Embodiment seven:
1. the Ganoderma spore of screening mature and plump is 100 kilograms, adds 300 kilograms of distilled water, and 32 ℃ of water temperatures were soaked 8 hours.
2. will pull out through the Ganoderma spore that soaks, and drip and remove excessive moisture, and be positioned over then and carry out the spore cultivation of sprouting in the heat and moisture preserving ventilation incubator.The incubator relative humidity is 95%, and cultivation temperature is 35 ℃, and the sprouting period time is 24 hours, and through electron microscopic examination, Ganoderma spore is in the state of sprouting, but does not stretch out germ tube as yet, and sporoderm is obviously softening.
3. handle through supermicro mill, wall breaking rate of ganoderma lucidum spores reaches 99%.
4. place conventional Minton dryer dried, finished product moisture content is less than 3.5%.
Claims (7)
1, a kind of glossy ganoderma spore activating produces the method for biological active substances, it is characterized in that the Ganoderma spore with mature and plump, through immersion induce accelerating germination, cultivations of sprouting, sporoderm processing, drying or the processing of lixiviate operation.
2, a kind of glossy ganoderma spore activating as claimed in claim 1 produces the method for biological active substances, it is characterized in that soaking when inducing accelerating germination and can add clear water, distilled water, normal saline or impel the nutritional solution of Ganoderma spore fast-germination: Cortex cocois radicis fruit caving water, the 1-5% malt extract, 0.5-25% Ganoderma sporophore or Ganoderma mycelium leachate, 0.1-5% biotin culture fluid, 0.1-3% potassium dihydrogen phosphate and magnesium sulfate culture fluid, can select wherein one or more for use, addition is 0.1-5 a times of Ganoderma spore weight, and soak time is to soak 30 minutes to 8 hours in 20-43 ℃ of water temperature.
3, a kind of glossy ganoderma spore activating as claimed in claim 1 or 2 produces the method for biological active substances, and the relative humidity of cultivating that it is characterized in that sprouting is 65-98%, and cultivation temperature is 20-48 ℃, and the sprouting period time is 30 minutes to 24 hours.
4, a kind of glossy ganoderma spore activating as claimed in claim 1 or 2 produces the method for biological active substances, it is characterized in that sporoderm is handled can adopt enzymolysis to reduce sporoderm toughness.Chitinase, cellulase degradation through commercial Application under low temperature environment make its sporoderm lose toughness and embrittlement; Adopting industrial micronizing, roll or grind machinery carries out the micronizing breaking cellular wall, rolls breaking cellular wall, grinds breaking cellular wall or super-voltage micro jet breaking cellular wall.
5, a kind of glossy ganoderma spore activating as claimed in claim 3 produces the method for biological active substances, it is characterized in that sporoderm is handled can adopt enzymolysis to reduce sporoderm toughness, and chitinase, cellulase degradation through commercial Application under low temperature environment make its sporoderm lose toughness and embrittlement; Also can adopt industrial micronizing, roll or grind machinery and carry out the micronizing breaking cellular wall, roll breaking cellular wall, grind breaking cellular wall or super-voltage micro jet breaking cellular wall.
6, a kind of glossy ganoderma spore activating as claimed in claim 1 or 2 produces the method for biological active substances, it is characterized in that drying) under cryogenic conditions, carry out, comprise and adopt industrial lyophilization, vacuum drying or cold drying; Lixiviate can adopt that industry water is carried, alcohol extraction or thin film concentration process are extracted physiologically active ingredient and be mixed with extractum, injection or oral liquid.
7, a kind of glossy ganoderma spore activating as claimed in claim 3 produces the method for biological active substances, it is characterized in that drying can carry out under cryogenic conditions, comprises and adopts industrial lyophilization, vacuum drying or cold drying; Lixiviate can adopt that industry water is carried, alcohol extraction or thin film concentration process are extracted physiologically active ingredient and be mixed with extractum, injection or oral liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98122269A CN1067587C (en) | 1998-12-30 | 1998-12-30 | Glossy ganoderma spore activating method to produce physiological active substance |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN98122269A CN1067587C (en) | 1998-12-30 | 1998-12-30 | Glossy ganoderma spore activating method to produce physiological active substance |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1230425A CN1230425A (en) | 1999-10-06 |
| CN1067587C true CN1067587C (en) | 2001-06-27 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN98122269A Expired - Lifetime CN1067587C (en) | 1998-12-30 | 1998-12-30 | Glossy ganoderma spore activating method to produce physiological active substance |
Country Status (1)
| Country | Link |
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| CN (1) | CN1067587C (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6316002B1 (en) * | 1999-10-12 | 2001-11-13 | Xin Liu | Germination activated red Ganoderma lucidum spores and method for producing the same |
| CN100372923C (en) * | 2006-01-20 | 2008-03-05 | 中山大学 | Method for promoting Chinese Ganoderma spore gemination |
| CN100593410C (en) * | 2006-05-24 | 2010-03-10 | 广东粤微食用菌技术有限公司 | Method for preparing ganoderma spore oil |
| CN102727535A (en) * | 2012-06-02 | 2012-10-17 | 郑家德 | Germination activation technique of ganoderma spores and extraction of active substances of ganoderma spores |
| CN104312921A (en) * | 2014-09-28 | 2015-01-28 | 广州英佩尔电子科技有限公司 | Comprehensive micro-molecule wall breaking method used for fresh food and drug materials |
| CN104783064A (en) * | 2015-04-06 | 2015-07-22 | 吉林农业大学 | Ganoderma spore germinating and wall breaking method |
| CN104857030A (en) * | 2015-04-30 | 2015-08-26 | 黄宇明 | Wall breaking method for ganoderma lucidum spore powder and product obtained with same |
| CN107893029B (en) * | 2017-12-29 | 2021-09-24 | 安徽金寨乔康药业有限公司 | Composite wall-breaking treatment method of ganoderma lucidum spore powder |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1134306A (en) * | 1995-04-22 | 1996-10-30 | 江苏省农业科学院原子能农业利用研究所 | Producing method for sporoderme-broken ganoderma lucidum sporopollen and its product |
| CN1165032A (en) * | 1996-05-10 | 1997-11-19 | 中国科学院大连化学物理研究所 | Method for breaking cell wall of Ganoderma lucidum spore |
-
1998
- 1998-12-30 CN CN98122269A patent/CN1067587C/en not_active Expired - Lifetime
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1134306A (en) * | 1995-04-22 | 1996-10-30 | 江苏省农业科学院原子能农业利用研究所 | Producing method for sporoderme-broken ganoderma lucidum sporopollen and its product |
| CN1165032A (en) * | 1996-05-10 | 1997-11-19 | 中国科学院大连化学物理研究所 | Method for breaking cell wall of Ganoderma lucidum spore |
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| CN1230425A (en) | 1999-10-06 |
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| C35 | Partial or whole invalidation of patent or utility model | ||
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Commission number: 4W02780 Conclusion of examination: On the basis of claim 1-3 submitted by the claimant in November 28, 2009, the patent right of invention No. 98122269.2 is valid. Decision date of declaring invalidation: 20100420 Decision number of declaring invalidation: 14703 Denomination of invention: Glossy ganoderma spore activating method to produce physiological active substance Granted publication date: 20010627 Patentee: Sun Yat-sen University |
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| C35 | Partial or whole invalidation of patent or utility model | ||
| IP01 | Partial invalidation of patent right |
Commission number: 4W02513 Conclusion of examination: Claim No. 98122269.2 of the patent right for invention No. 2 is invalid, and the patent right of the invention remains valid on the basis of the claim 3-7 of the authorized text. Decision date of declaring invalidation: 20090927 Decision number of declaring invalidation: 13829 Denomination of invention: Glossy ganoderma spore activating method to produce physiological active substance Granted publication date: 20010627 Patentee: Sun Yat-sen University |
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| CX01 | Expiry of patent term |
Granted publication date: 20010627 |
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| CX01 | Expiry of patent term |