CN106755354A - A kind of molecular labeling TaSnRK2.4A related to thousand grain weight of wheat and stalk soluble sugar content and its application - Google Patents
A kind of molecular labeling TaSnRK2.4A related to thousand grain weight of wheat and stalk soluble sugar content and its application Download PDFInfo
- Publication number
- CN106755354A CN106755354A CN201611102181.4A CN201611102181A CN106755354A CN 106755354 A CN106755354 A CN 106755354A CN 201611102181 A CN201611102181 A CN 201611102181A CN 106755354 A CN106755354 A CN 106755354A
- Authority
- CN
- China
- Prior art keywords
- wheat
- exit site
- measured
- genotype
- population
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241000209140 Triticum Species 0.000 title claims abstract description 89
- 235000021307 Triticum Nutrition 0.000 title claims abstract description 89
- 238000002372 labelling Methods 0.000 title abstract description 12
- 235000013339 cereals Nutrition 0.000 title abstract description 8
- 238000000034 method Methods 0.000 claims abstract description 29
- 238000001514 detection method Methods 0.000 claims abstract description 14
- 230000029087 digestion Effects 0.000 claims description 22
- 238000013467 fragmentation Methods 0.000 claims description 14
- 238000006062 fragmentation reaction Methods 0.000 claims description 14
- 238000012408 PCR amplification Methods 0.000 claims description 11
- 108090000623 proteins and genes Proteins 0.000 claims description 10
- 239000000463 material Substances 0.000 claims description 9
- 102000004190 Enzymes Human genes 0.000 claims description 4
- 108090000790 Enzymes Proteins 0.000 claims description 4
- 238000012216 screening Methods 0.000 claims description 3
- 102000054766 genetic haplotypes Human genes 0.000 abstract description 21
- 238000009395 breeding Methods 0.000 abstract description 8
- 230000001488 breeding effect Effects 0.000 abstract description 8
- 238000012098 association analyses Methods 0.000 abstract description 3
- 238000011160 research Methods 0.000 abstract description 3
- 239000003147 molecular marker Substances 0.000 abstract description 2
- 108020004414 DNA Proteins 0.000 description 27
- 230000009182 swimming Effects 0.000 description 5
- 102000001253 Protein Kinase Human genes 0.000 description 4
- 108060006633 protein kinase Proteins 0.000 description 4
- 102000053602 DNA Human genes 0.000 description 3
- 108020004682 Single-Stranded DNA Proteins 0.000 description 3
- 230000002411 adverse Effects 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 230000002349 favourable effect Effects 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 2
- 239000004473 Threonine Substances 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 230000007850 degeneration Effects 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 230000006353 environmental stress Effects 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 101150042690 Snrk gene Proteins 0.000 description 1
- 238000000540 analysis of variance Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 210000000349 chromosome Anatomy 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000003205 genotyping method Methods 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 230000002018 overexpression Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000000384 rearing effect Effects 0.000 description 1
- 230000010496 root system development Effects 0.000 description 1
- 230000008054 signal transmission Effects 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/172—Haplotypes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Immunology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of molecular labeling related to thousand grain weight of wheat and stalk soluble sugar content and its application.One mononucleotide polymorphism site, corresponding to sequence 1 from 5 ' ends the 30th, is found in wheat polymorphism colony.In natural population, this SNP is primarily present two kinds of haplotypes：Haplotype first (G) and haplotype second (A).Proved by association analysis, in the homozygosis type of both haplotypes, the wheat of haplotype first homozygosis, its mass of 1000 kernel and stalk soluble sugar content extremely significantly (or notable) are less than the wheat of haplotype second homozygosis.Present invention also offers the molecular labeling of the SNP.It is demonstrated experimentally that by the SNP described in detection, you can find mass of 1000 kernel and stalk soluble sugar content wheat higher.The present invention provides a new method for the molecular marker assisted selection breeding of wheat, significant in High-Yield Wheat Cultivar or research is cultivated.
Description
Technical field
It is related to thousand grain weight of wheat and stalk soluble sugar content the present invention relates to biological technical field, more particularly to one kind
Molecular labeling TaSnRK2.4A and its application.
Background technology
Wheat is one of main cereal crops in the world, and its production is closely related with mankind's grain security.Arid is shadow
Ring the main abiotic limiting factor of Wheat Production.Protein kinase is the hinge of adverse circumstance signal transmission network, in environment stress
Extremely important effect is played in responsing reaction.Sucrose non-fermented protein kinase (sucrose non-fermental
Protein kinase, SnRK) it is a class serine/threonine protein kitase, wherein SnRK2 participates in turning for various adverse circumstance signals
Lead, played a crucial role at aspects such as plant stress-resistance, root system development and crop yield formation.How SnRK2 to be used for degeneration-resistant
High yield crops rearing new variety, is not only molecular biologist and geneticist's focus of attention, is also that breeding man compels highly necessary
The problem of solution.The excellent allele of SnRK2 is excavated from Germplasm Resources of Farm Crop, then by conventional hybridization and molecular labeling
Assisted Selection is combined and is used, undoubtedly most direct, most effective, is also most easy received variety of crops improvement side
Method.Conventional breeding generally relies on Phenotypic Selection, although obtaining great success, takes time and effort, and breeding process is slow；Compare and
Speech, molecular marker assisted selection breeding can targetedly be selected from generation to generation early, and operation is simple, and can be efficient
Using favorable genes, the process of breeding can be dramatically speeded up.
Wheat TaSnRK2.4 genes participate in the response to various environment stresses, and its overexpression can significantly improve plant pair
Resistance (Maoet al.TaSnRK2.4, a SNF1-typeserine/threonine protein kinase of various adverse circumstances
of wheat(TriticumaestivumL.),confers enhanced multi-stress tolerances
inArabidopsis.J Exp Bot,2010,61:683-96), thus be the degeneration-resistant molecular breeding of wheat favorable genes resource,
But so far, the molecular labeling of TaSnRK2.4 genes is not yet developed, also have no way of finding out about it molecular labeling and economical character
Relation.
The content of the invention
It is an object of the present invention to provide a kind of method identified or aid in identification Traits of Wheat to be measured.
The method that the present invention is provided, comprises the following steps：The genotype of the exit site of wheat population genome to be measured is detected,
Genotype according to exit site determines that Traits of Wheat to be measured is as follows：
The genotype of exit site is the wheat population thousand of AA less than the genotype of exit site for the wheat population mass of 1000 kernel of GG
Weight；
And/or, the genotype of exit site is less than the genotype of exit site for the wheat population stalk soluble sugar content of GG
The wheat population stalk soluble sugar content of AA；
The exit site is sequence 1 the 30th from 5 ' ends, positioned at 3A chromosomes, gene regions the 2478th.
In the above method, the method for the genotype of the exit site of the detection wheat population genome to be measured includes following step
Suddenly：Enter performing PCR amplification including the DNA fragmentation including the exit site to any one section in the genomic DNA of wheat to be measured, and will
Pcr amplification product digestion is identified, obtained including the DNA fragmentation digestion products including exit site, true according to digestion products clip size
The genotype of the exit site of the fixed wheat population genome to be measured.
In the above method, in the genomic DNA to wheat to be measured any one section including the DNA including the exit site
Single strand dna of the primer pair shown in sequence 2 and the single strand dna shown in sequence 3 that fragment enters performing PCR amplification are constituted；
Enzyme used by the digestion of the DNA fragmentation including including the exit site is Hpa II.
In the above method, the E that the wheat population genome to be measured is determined according to the digestion products X clip sizes
The genotype in site is as follows：
If the digestion products X is only the DNA fragmentation of 212bp, the wheat to be measured is AA in the genotype of exit site；
If the digestion products X is the DNA fragmentation of 28bp, 184bp, the wheat to be measured is in the genotype of exit site
GG。
Another object of the present invention is to provide a kind of side of the genotype of the exit site for detecting wheat population genome to be measured
Method.
The method that the present invention is provided, including middle detection wheat population genome to be measured exit site genotype side
Method.
The 3rd purpose of the present invention is to provide a kind of product identified or aid in identification Traits of Wheat to be measured.
The product that the present invention is provided, to detect the material of the genotype of the exit site of wheat population genome to be measured.
In the said goods, the material includes primer pair 1 and the enzymes of Hpa II；
Single strand dna of the primer pair 1 shown in sequence 2 and the single strand dna shown in sequence 3 are constituted.
In the said goods, the product is kit.
The material of the genotype of the exit site of above-mentioned detection wheat population genome to be measured is being identified or is aiding in identification to be measured small
Application in wheat proterties is also the scope of protection of the invention；
Or, the material of the genotype of the exit site of above-mentioned detection wheat population genome to be measured is preparing identification or auxiliary mirror
Application in fixed Traits of Wheat product to be measured is also the scope of protection of the invention.
Application of the above method in the wheat that mass of 1000 kernel is high and/or stalk soluble sugar content is high is cultivated is also the present invention
The scope of protection.
The 4th purpose of the present invention is to provide a kind of screening or cultivation mass of 1000 kernel is high and/or stalk soluble sugar content is high
The method of wheat population.
The method that the present invention is provided, comprises the following steps：The genotype for screening or cultivating above-mentioned exit site is the wheat of AA
Colony.
The target sequence of the PCR amplifications is sequence 1 from 5 ' end 1-212bp.
It is confirmed as the wheat to be measured of genotype A, its mass of 1000 kernel and stalk soluble sugar content extremely significantly (or notable) are low
In the wheat to be measured for being confirmed as genotype B.
The present invention has found mononucleotide at 1 by the analysis of variance to TaSnRK2.4A genes in wheat polymorphism colony
Pleomorphism site, is named as exit site.The present invention develops dCAPS marks according to exit site, is named as AM.This SNP is in natural group
There are two kinds of haplotypes in body：Haplotype first (G) and haplotype second (A).Association analysis shows, the homozygosis class of both haplotypes
In type, the mass of 1000 kernel of haplotype first and stalk soluble sugar content extremely significantly (or notable) are less than haplotype second.It is demonstrated experimentally that logical
Cross the SNP described in detection, you can find mass of 1000 kernel and the of a relatively high wheat of stalk soluble sugar content.The present invention is wheat point
Sub- marker assisted selection breeding provides a new method, significant in High-Yield Wheat Cultivar or research is cultivated.
Brief description of the drawings
Fig. 1 is to develop molecular labeling digestion products electrophoresis detection result according to SNP of the invention.
Wherein, swimming lane M is 100bp DNA ladder.It is to be carried out with the PCR primer of II pair of different wheat of Hpa shown in figure
The banding pattern of digestion.Swimming lane A contains a band of 212bp；Swimming lane G contains two and is followed successively by 184bp's and 28bp from top to bottom
Band, because electrophoresis time is more long, the small fragment bar of 28bp is more obscured.
Specific embodiment
Experimental technique used in following embodiments is conventional method unless otherwise specified.
Material used, reagent etc. in following embodiments, unless otherwise specified, commercially obtain.
The present invention is further described in detail with reference to specific embodiment, the embodiment for being given is only for explaining
The bright present invention, rather than in order to limit the scope of the present invention.
The SNP related to thousand grain weight of wheat and stalk soluble sugar content of embodiment 1 and its mark polymorphic detection
1st, the special primer of genomic DNA fragment of the amplification containing wheat SNP site
Inventor's 32 parts of Guard cell kinds (coming from national germplasm resource bank) from table 1 are found that 1 change
Ectopic sites, it is molecular labeling to select this SNP to develop, and (E is named as from the 30th of 5 ' ends corresponding to sequence 1
Point, the polymorphism that there is A and G)；There are two kinds of haplotypes in wheat natural variation colony in this exit site：
Haplotype first：G；
Haplotype second：A.
1,32 parts of Guard cell kinds of table
According to the sequence difference of wheat different genes group, the PE amplifications of design genome specificity primer pair exist comprising exit site
Interior DNA fragmentation, primer pair PE is made up of the single stranded DNA of entitled pe1 and pe2, pe1 be sequence 2 (5 '-
CAAACTCACCTTTCCATCATACTCACGCC-3 ') shown in single stranded DNA, pe2 be sequence 3 (5 '-
ATATGCGATTTCGGCTACTCCAAGGTTG-3 ') shown in single stranded DNA.
2nd, sequence polymorphism detection and the foundation of methods of genotyping
1) genomic DNA of wheat to be measured is extracted, entering performing PCR with genome specific primer (F and R) expands, and obtains PCR expansions
Volume increase thing；
F:5 '-CATGAGTAAAACTTGCATACTATATTTCTC-3 ' (sequence 4)；
R:5 '-TCCGCGGCTCCGGC-3 ' (sequence 5).
2) with step 1) pcr amplification product dilute 50 times be template, using the primer pair PE in step 1 enter performing PCR expand
Increase, obtain pcr amplification product.
PCR amplification system be：The μ L of template DNA 2 (10pg-1 μ g), 2 × UtaqPCR MasterMix12.5 μ L, primer
Pe1 (10 μm of ol/L) and pe2 (10 μm of ol/L) each 1.0 μ L, mends ddH2The μ of O to 25.0 L.
PCR amplification conditions are:95 DEG C of 5min, 95 DEG C of 50s, 59 DEG C of 45s, 72 DEG C of 50s, 33 circulations；72 DEG C of 10min, 4 DEG C
Preserve.
3) by step 2) the PCR primer digestions of Hpa II, obtain digestion products；Digestion products carry out 4% agarose and coagulate
Gel electrophoresis detection, each clip size in identification record digestion products, and judged according to following method and record wheat to be measured in institute
State the situation of exit site：
If the digestion products are the DNA fragmentation of 212bp (the 1st -212bp of sequence 1), the wheat to be measured is in institute
State the wheat (the swimming lane A in Fig. 1) that exit site is A homozygosis (being expressed as A/A)；
If the digestion products are 184bp (the 29th -212bp of sequence 1), 28bp (the 1st -28bp of sequence 1)
DNA fragmentation, then the wheat to be measured is the wheat (the swimming lane G in Fig. 1) of G homozygosis (being expressed as G/G) in the exit site.
4) according to step 3) result, by wheat be divided into E the and F sites situation be following I-II type：
Ⅰ:G/G (i.e. haplotype first homozygosis)；
Ⅱ:A/A (i.e. haplotype second homozygosis).
It is the situation on a homologue before above-mentioned "/", is the feelings on another homologue after above-mentioned "/"
Condition.
3rd, parting is carried out to natural population using the molecular labeling and is entered with mass of 1000 kernel and stalk soluble sugar content proterties
Row association analysis
Distinguished with each wheat in the natural population that 262 parts of Guard cells (coming from national germplasm resource bank) are constituted
Parting is carried out according to the method for step 2 as wheat to be measured, the amplified production size to part wheat carries out sequence verification at random,
Result is as shown in table 2.
The situation statistics in E and F sites in table 2, wheat natural population
Note："-" is indicated without PCR primer.
With General linear model (GLM) models in Tassel2.1 softwares to two kinds of haplotypes and mass of 1000 kernel,
Stalk soluble sugar content proterties is associated analysis.
Result finds that the mass of 1000 kernel of haplotype I and stalk soluble sugar content extremely significantly (or notable) are less than haplotype
Ⅱ.Research to natural population shows that haplotype II is the favorable genes type for improving mass of 1000 kernel and stalk soluble sugar content.
Two kinds of genotype correlation shape statisticses in table 3, wheat natural population
Note：**Represent Traits change extremely significantly (P between different genetic wheat varieties<0.01).
Sequence table
<110>Institute of Crop Science, Chinese Academy of Agricultural Science
<120>A kind of molecular labeling TaSnRK2.4A related to thousand grain weight of wheat and stalk soluble sugar content and its application
<160> 5
<170> PatentIn version 3.5
<210> 1
<211> 212
<212> DNA
<213>Artificial sequence
<220>
<223>
<400> 1
caaactcacc tttccatcat actcacgccr ggatagcacc tctggtgcaa tatatgctgg 60
cgtccccact gctgatttgg gccttgaatg caatactgat gactgatcca gaaatgggaa 120
gaacagtttc tgtgagaatt tacagacatc gataacttga aggttcaata tgtgggttca 180
gggacaacct tggagtagcc gaaatcgcat at 212
<210> 2
<211> 29
<212> DNA
<213>Artificial sequence
<220>
<223>
<400> 2
caaactcacc tttccatcat actcacgcc 29
<210> 3
<211> 28
<212> DNA
<213>Artificial sequence
<220>
<223>
<400> 3
atatgcgatt tcggctactc caaggttg 28
<210> 4
<211> 30
<212> DNA
<213>Artificial sequence
<220>
<223>
<400> 4
catgagtaaa acttgcatac tatatttctc 30
<210> 5
<211> 14
<212> DNA
<213>Artificial sequence
<220>
<223>
<400> 5
tccgcggctc cggc 14
Claims (10)
1. a kind of method identified or aid in identification Traits of Wheat to be measured, comprises the following steps：Detect wheat population gene to be measured
The genotype of the exit site of group, the genotype according to exit site determines that Traits of Wheat to be measured is as follows：
The genotype of exit site is the wheat population mass of 1000 kernel of AA less than the genotype of exit site for the wheat population mass of 1000 kernel of GG；
And/or, the genotype of exit site is AA's less than the genotype of exit site for the wheat population stalk soluble sugar content of GG
Wheat population stalk soluble sugar content；
The exit site is sequence 1 the 30th from 5 ' ends.
2. method according to claim 1, it is characterised in that：The exit site of the detection wheat population genome to be measured
The method of genotype comprises the following steps：To any one section in the genomic DNA of wheat to be measured including including the exit site
DNA fragmentation enters performing PCR amplification, and by pcr amplification product digestion identification, obtains being produced including the DNA fragmentation digestion including exit site
Thing, the genotype of the exit site of the wheat population genome to be measured is determined according to digestion products clip size.
3. method according to claim 2, it is characterised in that：Any one section in the genomic DNA to wheat to be measured
Enter single strand dna and sequence 3 of the primer pair of performing PCR amplification as shown in sequence 2 including the DNA fragmentation including the exit site
Shown single strand dna composition；
Enzyme used by the digestion of the DNA fragmentation including including the exit site is Hpa II.
4. according to the method in claim 2 or 3, it is characterised in that：
The genotype of the exit site that the wheat population genome to be measured is determined according to the digestion products X clip sizes is
It is as follows：
If the digestion products X is only the DNA fragmentation of 212bp, the wheat to be measured is AA in the genotype of exit site；
If the digestion products X is the DNA fragmentation of 28bp, 184bp, the wheat to be measured is GG in the genotype of exit site.
5. a kind of method of the genotype of the exit site for detecting wheat population genome to be measured, including in claim 2-4 it is any in
Detection wheat population genome to be measured exit site genotype method.
6. it is a kind of identify or auxiliary identification Traits of Wheat to be measured product, to detect the exit site of wheat population genome to be measured
The material of genotype.
7. product according to claim 6, it is characterised in that：The material includes primer pair 1 and the enzymes of Hpa II；
Single strand dna of the primer pair 1 shown in sequence 2 and the single strand dna shown in sequence 3 are constituted.
8. the product according to claim 6 or 7, it is characterised in that：The product is kit.
9. the material of the genotype of the exit site of the detection wheat population genome to be measured during claim 6-8 is any is in mirror
Application in fixed or auxiliary identification Traits of Wheat to be measured；
Or, the material of the genotype of the exit site of detection wheat population genome to be measured during claim 6-8 is any is in system
Application in standby identification or auxiliary identification Traits of Wheat product to be measured.
10. in claim 1-4 any methods described in mass of 1000 kernel is high and/or stalk soluble sugar content is high wheat is cultivated
Application；
Or, a kind of method screened or cultivate the wheat population that mass of 1000 kernel is high and/or stalk soluble sugar content is high, including it is as follows
Step：Screening or the genotype for cultivating any described exit site in claim 1-4 are the wheat population of AA.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611102181.4A CN106755354B (en) | 2016-12-05 | 2016-12-05 | One kind molecular labeling TaSnRK2.4A relevant to thousand grain weight of wheat and stalk soluble sugar content and its application |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611102181.4A CN106755354B (en) | 2016-12-05 | 2016-12-05 | One kind molecular labeling TaSnRK2.4A relevant to thousand grain weight of wheat and stalk soluble sugar content and its application |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106755354A true CN106755354A (en) | 2017-05-31 |
| CN106755354B CN106755354B (en) | 2019-09-13 |
Family
ID=58883299
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611102181.4A Expired - Fee Related CN106755354B (en) | 2016-12-05 | 2016-12-05 | One kind molecular labeling TaSnRK2.4A relevant to thousand grain weight of wheat and stalk soluble sugar content and its application |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106755354B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111663004A (en) * | 2020-07-28 | 2020-09-15 | 安徽农业大学 | Method for identifying or assisting in identifying strength of wheat stalks and application of method |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102220297A (en) * | 2011-05-31 | 2011-10-19 | 中国农业科学院作物科学研究所 | Stress resistance associated protein TaSnRK2.3 and coding gene and use thereof |
| CN102399760A (en) * | 2011-10-28 | 2012-04-04 | 中国农业科学院作物科学研究所 | Plant stress tolerance related protein TaSnRK2.10 as well as coding gene and application thereof |
| CN103820476A (en) * | 2014-01-24 | 2014-05-28 | 山东农业大学 | Gene relevant to wheat thousand seed weight, functional marker and application thereof |
| CN104342484A (en) * | 2013-07-23 | 2015-02-11 | 中国农业科学院作物科学研究所 | Molecular marker related with wheat thousand grain weight and applications thereof |
-
2016
- 2016-12-05 CN CN201611102181.4A patent/CN106755354B/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102220297A (en) * | 2011-05-31 | 2011-10-19 | 中国农业科学院作物科学研究所 | Stress resistance associated protein TaSnRK2.3 and coding gene and use thereof |
| CN102399760A (en) * | 2011-10-28 | 2012-04-04 | 中国农业科学院作物科学研究所 | Plant stress tolerance related protein TaSnRK2.10 as well as coding gene and application thereof |
| CN104342484A (en) * | 2013-07-23 | 2015-02-11 | 中国农业科学院作物科学研究所 | Molecular marker related with wheat thousand grain weight and applications thereof |
| CN103820476A (en) * | 2014-01-24 | 2014-05-28 | 山东农业大学 | Gene relevant to wheat thousand seed weight, functional marker and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| XINGUO MAO等: "TaSnRK2.4, an SNF1-type serine/threonine protein kinase of wheat (Triticum aestivum L.), confers enhanced multistress tolerance in Arabidopsis", 《JOURNAL OF EXPERIMENTAL BOTANY》 * |
| 张洪映等: "植物和小麦SnRK2基因家族的研究进展", 《安徽农业科学》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111663004A (en) * | 2020-07-28 | 2020-09-15 | 安徽农业大学 | Method for identifying or assisting in identifying strength of wheat stalks and application of method |
| CN111663004B (en) * | 2020-07-28 | 2022-03-22 | 安徽农业大学 | Method for identifying or assisting in identifying strength of wheat stalks and application of method |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106755354B (en) | 2019-09-13 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105385697B (en) | Sesame inflorescence finite basis is because of Sidt1 and its SNP marker | |
| CN102162011B (en) | Molecule marking method of rice blast-resisting gene | |
| CN102154281B (en) | Molecular marker SIsv0010 closely linked with heading-date gene of millet | |
| CN110117673A (en) | The molecular labeling of the short bar character site of cabbage type rape and its application | |
| CN105713971A (en) | InDel molecular marker for identifying watermelon seed size and primers and application thereof | |
| CN106381343B (en) | One kind molecular labeling TaSnRK2.3A relevant to thousand grain weight of wheat and plant height and its application | |
| CN104152450B (en) | The InDel molecular labelings isolated with cucumber powdery mildew resistance main effect gene | |
| CN109652412A (en) | The method and application of a kind of SNP marker, detection muskmelon flower property type | |
| CN106755355B (en) | One kind molecular labeling TaSnRK2.3B relevant to thousand grain weight of wheat and stalk soluble sugar content and its application | |
| CN106755359B (en) | Method for screening or auxiliary screening of wheat with different plant heights and special kit thereof | |
| CN103789419B (en) | Co-dominance tag primer group for identifying allele type of rice photo-thermo-sensitive genic male-sterile gene p/tms12-1, and applications thereof | |
| CN114107537B (en) | Molecular marker closely linked with main effect QTL of lateral root number in wheat seedling stage, detection primer and application thereof | |
| CN109609687A (en) | KASP labeled primer for detecting watermelon blight resistance combines and its application | |
| CN106119350A (en) | Identify ' Hort 16A ' Fructus actinidiae chinensis opening pollination offspring's property method for distinguishing in early days | |
| CN111621586B (en) | SNP molecular marker closely linked with pumpkin yellow stem character and application thereof | |
| CN105734145A (en) | Strawberry anthocyanin regulatory gene functional marker | |
| CN107586874A (en) | For identifying primer pair and its application of capsicum yellow flower pesticide character | |
| CN107447026A (en) | A kind of specific molecular marker primer of brown planthopper resistant gene in rice BPH3 genotype identifications and its application | |
| CN106755354B (en) | One kind molecular labeling TaSnRK2.4A relevant to thousand grain weight of wheat and stalk soluble sugar content and its application | |
| CN110093435B (en) | Wheat SSR molecular marker primer and screening method thereof | |
| CN107619875B (en) | Insertion deletion marker locus for identifying watermelon fruit shape, primer and application | |
| CN105713983B (en) | A kind of molecular labeling and its application with rice Jiangnan evening neck blast resistance gene close linkage | |
| CN114317812A (en) | Rice salt tolerance related molecular marker and application thereof | |
| CN108034654B (en) | SNP molecular marker related to rice seedling stage root length and application thereof | |
| CN107130019A (en) | The molecular labeling primer of the thin spike of rice pest resistant gene of Local Rice Race and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190913 |