CN106755340A - A kind of method and system for carrying out Y STR partings to male individual using 26 Y str locus seats - Google Patents
A kind of method and system for carrying out Y STR partings to male individual using 26 Y str locus seats Download PDFInfo
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
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- C12Q2600/00—Oligonucleotides characterized by their use
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- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Abstract
The present invention provides a kind of method and system for carrying out Y STR partings to male individual using 26 Y str locus seats,The method includes obtaining the DNA of the male individual,Obtain 26 genotype of Y str locus seat of the DNA,26 Y str locus seat is DYS19,DYS385a/b,DYS389I/II,DYS390,DYS391,DYS392,DYS393,DYS437,DYS448,DYS456,DYS458,DYS635,GATA H4,DYS438,DYS439,DYS460,DYS447,DYS527a/b,DYS617,DYS522,DYS444,DYS508 and DYS533；Genotype according to 26 locus of the male individual obtains the individual Y STR genotyping results.The detection of male's composition in family investigation, patriarchy paternity identification, men and women's mixing sample and the detection of male's composition in multiple male's mixing sample etc. can be carried out to the male individual using the genotyping result.
Description
Technical field
It is individual to male using 26 Y-STR locus the present invention relates to a kind of classifying method and system, more particularly to one kind
Body carries out the method and system of Y-STR partings.
Background technology
Tandem repetitive sequence (short tandem repeat, STR) is be widely present in human chromosomal DNA
Class polymorphic markers system, because its existence range is wide (having a str locus seat in average 16kb), core sequence is small
(2-7bp) and amplified production length are respectively less than 500bp, and the numeral in allele site is to represent the number of times that sequence is repeated.STR bases
Because the allele fragment length of seat is concentrated, therefore composite amplification can be carried out to multiple str locus seat.Composite amplification multiple STR bases
Because of seat, accumulative distinguishing ability can be close to or up DNA fingerprint level.
Y chromosome is the distinctive chromosome of male, and same paternal all of male individual all has mono- times of identical Y-STR
Type.A kind of means of supplementing out economy that Y-STR inspections are checked as autosome, are mixed into family investigation, patriarchy paternity identification, men and women
The detection of male's composition and the judgement of multiple male's blending constituents have important application value, and because case-involving crime in point
Involved party more than 90% is male, and Y-STR inspections play more and more important role in DNA inspections.
The effect of present forensic dna technology is more embodied in passive comparison aspect, and Y-STR methods of inspection are introduced into investigation
Field is cleared up a cace, and has founded " Y-STR familys investigate method ", can reduce homicide case scope of investigation, for investigation proposes directive property
Clue.Family investigation refers to the Y-STR data for extracting scene of a crime, compares with a range of family, once
Than in, family where criminal offence people just can be pointed to.
Family collection of illustrative plates is drawn in current Henan, Shandong etc. with county (city) for unit builds storehouse by unit of administrative village, is in
It is that biological specimen is gathered on the basis of collection of illustrative plates, carries out Y-STR database establishments work.Automated by Y data storehouse and compared, can be fast
Speed points to family where suspect, reduces scope of investigation；It is quick to point to unknown dead body place family, indicate investigation direction；
The interference of women DNA can be excluded, clear and definite male's information is obtained, automatic comparison is put in storage.Henan Y storehouses take " built when beating, with
Beat rush build " policy, crack hundreds of homicide case long-pending cases using Kucheng's work(, it is quite effective.
A kind of Y-STR fluorescent composite amplifications for legal medical expert Y-STR detections are disclosed in patent CN103866019 and checks examination
Agent, specifically discloses 21 Y-STR locus and amplimer.However, the design of its primer causes some Y-STR locus
The amplified production of amplified production arrangement too closely even previous locus is cross in the range of next locus, is caused point
Type cannot interpretation, in addition locus arrangement closely may also result in allelic ladder analysis when there is crosstalk, mistake
Position is so as to cause the whole result cannot Correct Analysis interpretation.
How to provide a kind of method and system that Y-STR partings are carried out to male individual for solving the above problems turn into have
Problem to be solved.
The content of the invention
The invention provides a kind of method that Y-STR partings are carried out to male individual using 26 Y-STR locus, pass through
The specific primer for being combined using specific Y-STR locus and being designed for these locus so that will not during parting
Occur parting cannot interpretation, parting crosstalk, dislocation problem, can exactly obtain 26 bases of Y-STR locus of male individual
Because of type, so as to realize carrying out the male individual male's composition in family investigation, patriarchy paternity identification, men and women's blending constituent
Detection and the judgement of multiple male's blending constituents etc. provide data and support.
The present invention also provides a kind of system that Y-STR partings are carried out to male individual using 26 Y-STR locus, passes through
The system can realize being directed to male individual the accurate parting of above-mentioned 26 locus.
Present invention also offers a kind of compound detection system, the detection architecture can accurately obtain 26 Y- of male individual
The genotype of str locus seat.
Present invention also offers a kind of detection kit, including described compound detection system.
A kind of method that Y-STR partings are carried out to male individual using 26 Y-STR locus that the present invention is provided, the party
Method includes：
1) DNA of male individual is obtained；
2) obtain the genotype of the DNA26 Y-STR locus, 26 Y-STR locus be DYS19,
DYS385a/b、DYS389I/II、DYS390、DYS391、DYS392、DYS393、DYS437、DYS448、DYS456、DYS458、
DYS635、GATA H4、DYS438、DYS439、DYS460、DYS447、DYS527a/b、DYS617、DYS522、DYS444、
DYS508 and DYS533, including use amplimer corresponding with the locus to be expanded to obtain amplified production to it
Step；
Wherein the amplimer of locus DYS460 corresponds to the nucleotide sequence of SEQ ID No.1 to SEQ ID No.2；
The amplimer of locus DYS389I/II is identical, is the nucleotide sequence of SEQ ID No.3 to SEQ ID No.4；Locus
The amplimer of DYS390, DYS392, DYS458, DYS437 corresponds respectively to the core of SEQ ID No.5 to SEQ ID No.12
Nucleotide sequence；The amplimer of locus DYS385a/b is identical, is the nucleotides sequence of SEQ ID No.13 to SEQ ID No.14
Row；Locus GATA-H4, DYS522, DYS456, DYS391, DYS447, DYS438, DYS448, DYS393, DYS635,
The amplimer of DYS439, DYS19, DYS444 corresponds respectively to the nucleotides sequence of SEQ ID No.15 to SEQ ID No.38
Row；The amplimer of locus DYS527a/b is identical, is the nucleotide sequence of SEQ ID No.39 to SEQ ID No.40；Base
Because of seat DYS617, the amplimer of DYS508, DYS533 corresponds respectively to the nucleosides of SEQ ID No.41 to SEQ ID No.46
Acid sequence；
3) genotype according to 26 locus of the male individual obtains the individual Y-STR genotyping results.
In the solution of the present invention, 26 locus be applicant by the living environment to a large amount of male individuals,
Ethnic origin etc. carries out comprehensive analysis acquisition.Primer for said gene seat is also that applicant is obtained by many experiments
, successfully solve to male individual Y-STR partings process exist parting cannot interpretation, parting crosstalk, dislocation ask
Topic, while the combination of these specific Y chromosome locus can also be realized carrying out male individual in family investigation, patriarchy parental right mirror
Male's ingredient origin is inferred in fixed, men and women's mixing sample, and/or male's ingredient origin is inferred in man's man's mixing sample.
Further, in the scheme of the application, " obtaining the DNA of male individual " refers to extracting such as blood of the individuality
DNA in liquid, tissue equal samples, or directly obtain the blood card containing the individual DNA." Y-STR of the male individual points
Type result " refer to being used for carrying out the male individual in family investigation, patriarchy paternity identification, men and women's mixing sample male into
Divide the Y-STR genotyping results of male's ingredient origin deduction in source deduction, and/or man's man's mixing sample.Wherein male aggregate sample
Male's ingredient origin infers the differentiation referred to the male individual from non-same patrilineal family in this.
In the specific embodiment of the present invention, the primer is fluorescent dye primer.
In another embodiment of the invention, wherein after 2) being additionally included in acquisition amplified production, using heredity
Analyzer analyzes the amplified production, the step of with the genotype for obtaining 27 locus.It is described in the solution of the present invention
Genetic analyzer can be the conventional use of genetic analyzer of those skilled in the art, such as ABI3130 or ABI3500 types heredity
Analyzer, passes throughID-X softwares or other GeneMapper softwares etc. analyze institute in the pcr amplification product
State 26 genotype of locus.
A kind of system that Y-STR partings are carried out to male individual using 26 Y-STR locus that the present invention is provided, it is described
System includes that DNA obtains system, compound detection system, deduction system；
The DNA obtains system is used to obtain the DNA of the male individual；
The compound detection system is used to obtain 26 genotype of Y-STR locus of the DNA, and according to the gene
Type obtains the individual Y-STR genotyping results,
26 Y-STR locus be DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392,
DYS393、DYS437、DYS448、DYS456、DYS458、DYS635、GATA H4、DYS438、DYS439、DYS460、
DYS447, DYS527a/b, DYS617, DYS522, DYS444, DYS508 and DYS533, obtain the gene of 26 locus
The process of type includes the step of using amplimer corresponding with the locus to expand to obtain amplified production it；
Wherein the amplimer of locus DYS460 corresponds to the nucleotide sequence of SEQ ID No.1 to SEQ ID No.2；
The amplimer of locus DYS389I/II is identical, is the nucleotide sequence of SEQ ID No.3 to SEQ ID No.4；Locus
The amplimer of DYS390, DYS392, DYS458, DYS437 corresponds respectively to the core of SEQ ID No.5 to SEQ ID No.12
Nucleotide sequence；The amplimer of locus DYS385a/b is identical, is the nucleotides sequence of SEQ ID No.13 to SEQ ID No.14
Row；Locus GATA-H4, DYS522, DYS456, DYS391, DYS447, DYS438, DYS448, DYS393, DYS635,
The amplimer of DYS439, DYS19, DYS444 corresponds respectively to the nucleotides sequence of SEQ ID No.15 to SEQ ID No.38
Row；The amplimer of locus DYS527a/b is identical, is the nucleotide sequence of SEQ ID No.39 to SEQ ID No.40；Base
Because of seat DYS617, the amplimer of DYS508, DYS533 corresponds respectively to the nucleosides of SEQ ID No.41 to SEQ ID No.46
Acid sequence；
The deduction system be used for according to the individual Y-STR genotyping results male individual is carried out family investigation,
Male's ingredient origin is inferred in patriarchy paternity identification, men and women's mixing sample, and/or male's ingredient origin is pushed away in man's man's mixing sample
It is disconnected.
In the specific embodiment of the present invention, the primer is fluorescent dye primer.
Further, the compound detection system is additionally operable to after amplified production is obtained, and is analyzed using genetic analyzer
The amplified production, to obtain the genotype of 26 locus.
Further, the genetic analyzer is determined using the parting standard thing of the allele for each locus
The genotype of each locus in male individual DNA.
The present invention provide a kind of compound detection system, the system include male individual DNA, 26 locus, and
Amplimer,
The compound detection system is used to be obtained using the amplimer amplification of the DNA26 Y-STR locus
Product, and 26 genotype of locus of the DNA of male individual are obtained by the amplified production, and further by the genotype
The individual Y-STR genotyping results are obtained,
26 Y-STR locus be DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392,
DYS393、DYS437、DYS448、DYS456、DYS458、DYS635、GATA H4、DYS438、DYS439、DYS460、
DYS447, DYS527a/b, DYS617, DYS522, DYS444, DYS508 and DYS533,
The amplimer is made up of amplimer corresponding with 26 locus, the wherein expansion of locus DYS460
Increase the nucleotide sequence that primer corresponds to SEQ ID No.1 to SEQ ID No.2；The amplimer phase of locus DYS389I/II
Together, it is the nucleotide sequence of SEQ ID No.3 to SEQ ID No.4；Locus DYS390, DYS392, DYS458, DYS437's
Amplimer corresponds respectively to the nucleotide sequence of SEQ ID No.5 to SEQ ID No.12；The amplification of locus DYS385a/b
Primer is identical, is the nucleotide sequence of SEQ ID No.13 to SEQ ID No.14；Locus GATA-H4, DYS522,
The amplification of DYS456, DYS391, DYS447, DYS438, DYS448, DYS393, DYS635, DYS439, DYS19, DYS444 is drawn
Thing corresponds respectively to the nucleotide sequence of SEQ ID No.15 to SEQ ID No.38；The amplimer of locus DYS527a/b
It is identical, it is the nucleotide sequence of SEQ ID No.39 to SEQ ID No.40；The expansion of locus DYS617, DYS508, DYS533
Increase the nucleotide sequence that primer corresponds respectively to SEQ ID No.41 to SEQ ID No.46.
In the solution of the present invention, the archaeal dna polymerase can be Fast Start archaeal dna polymerases, TaqDNA polymerizations
One or more in enzyme, Hotstart archaeal dna polymerases.
Present invention also offers a kind of detection kit, including described compound detection system.
In the solution of the present invention, the present invention carries out 26 sides of the parting of locus using the compound detection system
Method, including：1) the male individual DNA that will be obtained is used as template；2) amplimer is used as the male individual DNA of template
Carry out multiplexed PCR amplification and be reacted to give amplified production；3) amplified production is analyzed using genetic analyzer, to obtain
Obtain 26 genotype of locus.
In the solution of the present invention, the 26 locus information is as shown in table 1：
Table 1
| Locus | Repetitive sequence | 5 ' marks |
| DYS19 | TAGA | ROX |
| DYS385a/b | GAAA | HEX |
| DYS389I/II | (TCTG)(TCTA) | FAM |
| DYS390 | (TCTA)(TCTG) | FAM |
| DYS391 | TCTA | TAMRA |
| DYS392 | TAT | FAM |
| DYS393 | AGAT | ROX |
| DYS437 | TCTA | HEX |
| DYS448 | AGAGAT | TAMRA |
| DYS456 | AGAT | TAMRA |
| DYS458 | GAAA | HEX |
| DYS635 | TSTA | ROX |
| GATA-H4 | TAGA | HEX |
| DYS438 | TTTTC | TAMRA |
| DYS439 | AGAT | ROX |
| DYS460 | ATAG | FAM |
| DYS447 | TAAWA | TAMRA |
| DYS527ab | GAAA | ROX |
| DYS617 | TAT | TAMRA |
| DYS522 | GATA | HEX |
| DYS444 | TAGA | ROX |
| DYS508 | TATC | HEX |
| DYS533 | ATCT | FAM |
The amplimer sequence that the present invention is provided is as follows.The amplimer and its corresponding locus such as table 2 below institute
Show, PCRU represents sense primer, PCRL represents anti-sense primer；
Table 2
The present invention program has the following advantages that：
1st, detection method of the invention and detecting system are using the combination of specific str locus seat and primer sequence so that
Arrangement is uniform between each site on the final parting collection of illustrative plates for obtaining, and primer specificity is high, can obtain complete STR partings,
Peak type is sharply clear, and balance is good, and without Pull-up peaks, stutter bands, specific artefact appearance, is fully able to full nothing but
The requirement of sufficient legal medical expert Y-STR inspections.
2nd, detect that specific 26 Y-STR sites have parental right high simultaneously using detection method of the invention and detecting system
Male's ingredient origin is inferred during identification capacity, male individual carry out family investigation and men and women's mixing sample, and/or man man is mixed
Male's ingredient origin is inferred in closing sample.
3rd, the solution of the present invention can be by using fluorescence labeling, using conventional genetic analyzer, according to being expanded
Gene molecular weight and the difference of fluorescence color, obtain intuitively testing result, and the result and existing detecting system phase
Than accuracy is 100%.
4th, the scheme that the present invention is provided can be that its family investigation, paternity identification etc. provide accurate section effectively from gene level
Learn foundation.
Brief description of the drawings
Fig. 1 shows allele layout viewing (the wherein first behavior blue-fluorescence, the second behavior of each locus of the application
Green fluorescence, the third line is yellow fluorescence, and fourth line is red fluorescence).
Fig. 2 shows the genotyping result figure of the sample obtained using present system.
Fig. 3 shows dividing for the allelic ladder (ladder) obtained using patent CN103866019 systems
(wherein the first behavior blue-fluorescence, the second behavior green fluorescence, the third line is black fluorescent to type figure, and fourth line is red glimmering
Light).
Fig. 4 shows the parting figure of the allelic ladder (ladder) obtained using the application system (wherein
First behavior blue-fluorescence, the second behavior green fluorescence, the third line is black fluorescent, and fourth line is red fluorescence).
Fig. 5 shows the locus DYS460 allelic gene typings figure for using patent CN103866019 systems to obtain (wherein
First behavior blue-fluorescence, the second behavior green fluorescence, the third line is black fluorescent, and fourth line is red fluorescence).
Specific embodiment
The 193 parts of people's anticoagulations (193 parts of male) used in following examples, are provided by Material Evidence Identification Center, Ministry of Public Security.
The method used in following examples is conventional method unless otherwise instructed, and agents useful for same consumptive material and instrument are as follows
Shown in table 3：
Table 3
Embodiment 1, the checking to the method and system accuracy that Y-STR partings are carried out to male individual of the invention
In the present embodiment, the male individual is 193 parts of male people's anticoagulations, it is known that its individuality source, but in this Shen
Its individuality source please be set in the implementation process of embodiment 1 unknown, Y-STR points is carried out to it using the application method and system
Type, including：
1) DNA that system obtains male individual, 2 are obtained using the DNA in system of the invention) utilize system of the invention
In compound detection system obtain described 26 genotype of Y-STR locus of DNA, and the individuality is obtained according to the genotype
Y-STR genotyping results, 3) infer system using described in system of the invention, according to the individual Y-STR genotyping results
Male's ingredient origin in family investigation, patriarchy paternity identification, men and women's mixing sample is carried out to the male individual, and/or men and women mixes
Male's ingredient origin is inferred in closing sample.
In the present embodiment, the compound detection system is used to obtain described 26 Y-STR of DNA using the amplimer
The amplified production of locus, and 26 genotype of locus of the DNA of male individual are obtained by the amplified production, go forward side by side one
Walk and the individual Y-STR genotyping results are obtained by the genotype；
26 Y-STR locus be DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392,
DYS393、DYS437、DYS448、DYS456、DYS458、DYS635、GATA H4、DYS438、DYS439、DYS460、
DYS447, DYS527a/b, DYS617, DYS522, DYS444, DYS508 and DYS533；
The amplimer is made up of amplimer corresponding with 26 locus, the wherein expansion of locus DYS460
Increase the nucleotide sequence that primer corresponds to SEQ ID No.1 to SEQ ID No.2；The amplimer phase of locus DYS389I/II
Together, it is the nucleotide sequence of SEQ ID No.3 to SEQ ID No.4；Locus DYS390, DYS392, DYS458, DYS437's
Amplimer corresponds respectively to the nucleotide sequence of SEQ ID No.5 to SEQ ID No.12；The amplification of locus DYS385a/b
Primer is identical, is the nucleotide sequence of SEQ ID No.13 to SEQ ID No.14；Locus GATA-H4, DYS522,
The amplification of DYS456, DYS391, DYS447, DYS438, DYS448, DYS393, DYS635, DYS439, DYS19, DYS444 is drawn
Thing corresponds respectively to the nucleotide sequence of SEQ ID No.15 to SEQ ID No.38；The amplimer of locus DYS527a/b
It is identical, it is the nucleotide sequence of SEQ ID No.39 to SEQ ID No.40；The expansion of locus DYS617, DYS508, DYS533
Increase the nucleotide sequence that primer corresponds respectively to SEQ ID No.41 to SEQ ID No.46.
1st, 193 parts of blood cards containing above-mentioned 193 male individual DNA are obtained as experiment sample, and uses standard DNA 9948
As positive control.
2nd, 26 partings of locus are carried out to above-mentioned sample using the compound detection system, including：Use the expansion
Increasing primer pair DNA profiling carries out multiplexed PCR amplification, to obtain amplified production；Amplified production is determined 26 using genetic analyzer
The genotype of individual locus.Detailed process is as follows：
2.1st, primer pond configuration
The configuration in amplimer pond, wherein the corresponding amplimer of 26 locus is as described above；The present invention is provided
Various primer sequences synthesized by Shanghai Sangon Biological Engineering Technology And Service Co., Ltd.
Synthetic primer is diluted to 100 μM with 1 × TE buffer solutions, by 26 upstream and downstream primers of locus etc. than mixed
Close, obtain the primer that concentration is 50 μM.Different volumes are taken respectively from 23 pipe PCR primers to be added in a new centrifuge tube,
Used as 23 heavy PCR primer ponds, in the primer pond, the final concentration of the primer of each STR bit point is as shown in table 4 below：
Table 4
| Str locus seat | Final concentration (μm ol/L) |
| DYS460 | 0.15 |
| DYS389I/II | 0.3 |
| DYS390 | 0.23 |
| DYS533 | 0.2 |
| DYS392 | 0.5 |
| DYS508 | 0.3 |
| DYS437 | 0.3 |
| DYS385a/b | 0.3 |
| GATA-H4 | 0.3 |
| DYS522 | 0.3 |
| DYS456 | 0.15 |
| DYS458 | 0.2 |
| DYS391 | 0.24 |
| DYS447 | 0.26 |
| DYS438 | 0.4 |
| DYS448 | 0.2 |
| DYS617 | 0.5 |
| DYS393 | 0.15 |
| DYS635 | 0.3 |
| DYS439 | 0.3 |
| DYS19 | 0.4 |
| DYS444 | 0.26 |
| DYS527a/b | 0.34 |
2.2nd, multi-PRC reaction
The present embodiment carries out multi-PRC reaction using 9700 type PCR amplification instruments.
(1) configuration PCR mix (25 μ L systems), as shown in table 5 below.
Table 5
| Reagent name | Concentration |
| PCR primer pond | 12.5μL |
| Tris-HCl | 20mM |
| KCl | 50mM |
| MgCl2 | 1.6mM |
| Bovine serum albumin(BSA) | 0.8mg/ml |
| Tween-20 | 0.2% |
| Glycerine | 3.2% |
| NaN3 | 0.02% |
| dNTP | 200μM |
| Taq archaeal dna polymerases | 2U |
| Male individual DNA profiling card | 0.25mm2 |
| Amount to | 25μL |
(2) amplification program
The thermal circulation parameters of PCR amplification procedures are：1. 95 DEG C, 11min；2. 28 circulations, each circulates 94 DEG C of 1min, 60
DEG C 1min, 72 DEG C of 1min；3. 60 DEG C, 60min；4. 25 DEG C, insulation.
2.3rd, PCR primer parting
Treat the preparation of parting sample：
1. the preparation of electrophoresis loading mixture, prepares internal standard and deionized formamide composition loading mixes by following ratio
Thing：The μ l deionized formamides of 10 μ l Typer500 internal standards+1000, are well mixed.
2. often pipe adds 10 μ l loadings mixtures, 1 μ l amplified productions, mixes.
3. 95 DEG C be denatured 3 minutes, be immediately placed at cooled on ice 3 minutes, rear electrophoresis.
Detected on ABI3130XL type genetic analyzers.Using ABI 3130XL Date Collection
Software 3.1 collects data, and the softwares of GeneMapper 3.3 are analyzed to electrophoresis result, obtains 26 locus
Genotype.
2.4th, interpretation of result
193 parts of DNA samples are carried out with a representative genotyping result of parting as shown in Fig. 2 being using present system
The accuracy of checking genotyping result, randomly selects 100 parts of DNA samples from 193 parts of DNA samples, and 26 locus are carried out
Sequencing (sequencing of Beijing Mai Aodeen bio tech ltd), using the present embodiment compound detection system acquisition it is all of
Genotyping result is consistent with sequencing result, and uniformity reaches 100%, and it is accurate that this result demonstrate,proves compound detection system genotyping result of the present invention
Really.
3rd, the genotype according to 26 locus of the male individual obtains the individual Y-STR genotyping results, and carries out
Paternal relative identifies.Paternal relative's qualification result of the above-mentioned 193 parts of samples obtained by the present embodiment method, with its known
Body paternal relative qualification result is consistent, illustrates that the inventive method can carry out paternal relative's identification of male individual.
Further, the genotype of above-mentioned 26 locus can be used for carrying out family investigation, men and women to male individual
Male's ingredient origin is inferred in mixing sample, and/or male's ingredient origin is inferred in man's man's mixing sample.
Embodiment 2
The application Y-STR classification systems with it is existingPlus kits are in family judgement
Compare
Y-STR classification systems of the invention increased 5 Y-STR sites compared to patent CN103866019 detection architectures,
The resolution capability to different family males is increased, the increased Y-STR sites of institute belong to low mutation rate site in addition, so this
26 Y-STR sites low mutation rate site in belonging in invention system, is more suitable for family investigation, because mutational site high
It is easily caused the error exception in family investigation.
It is exemplified below：
On July 30th, 2016, Ruzhou City of Henan Province paper mill town head's East Village Ma (female, 12 years old) is raped and is murdered, in postmortem
During be extracted vaginal swab and anal swab, seminal stain preliminary experiment is the positive, and DNA inspections are carried out through to two parts of samples, in
On July 31st, 2016 obtains the euchromatic dna and Y chromosome DNA collection of illustrative plates of same male.Because this case has no clear and definite suspicion
People, and crime ground is located in remote, and population from other places is few, analyzes this case infringement object clearly, should be native's crime possibility big.
Therefore, application Y-STR family investigation methods are determined, to being investigated around crime ground.The deployment per stirpes collection at once of special project group
Male members blood sample in village around crime ground, by the end of August in 2016 3 days, 1400 is checked using the application Y-STR classification systems
Remaining part blood sample.Investigate to Ruzhou City Zhi Fang towns Xi Zhaoluo villages Min self-insurance blood sample Y-STR partings and 26 sites of material evidence Y-STR partings
In only DYS527ab sites exist an one-step mutation, it is impossible to exclude the suspicion of Min surname family.
With commercially availablePlus kits are further tested Min Zibao blood samples and material evidence
Card, it is found that the two has mutation on three sites, and wherein DYS627 and DYS570 belongs to one-step mutation, and DYF387S1 is two steps
Mutation.Statistics is as shown in the table, and Min Zibao blood samples have mutation with material evidence on four sites, and in general, tolerance is big
In 3, then the family suspicion can be excluded, that is, usedPlus kits most probably can be by Min surname family
The exclusion of mistake.
But we carefully analyze, there are three to belong to rapid mutation site in four sites undergone mutation of the above, and
And except DYF387S1 is that DYS570, DYS627, DYS527ab belong to one-step mutation in addition to two steps are mutated, so we judge still
The suspicion of this family can not so be excluded.
On the one hand special project group investigates Min Zibao familys using the application Y-STR classification systems, on the one hand to all Mins in the village
Surname personnel's blood sampling examination, finally locks the artificial Min system family member Min Jun of suspicion and stops.
Following table is the genotyping result of DNATyperY26 kits, and FW is material evidence, and T1 is Min Zibao.
The parting of plus kits, FW is material evidence, and T1 is Min Zibao.
The comparing of detection architecture in the detecting system of the present invention of embodiment 3 and patent CN103866019
1. the primer in detecting system of the present invention and patent CN103866019 detection architectures designed by each locus expands
The amplified production magnitude range for increasing each allele is as follows：
Patent CN103866019：
The application (where figure 1 shows the amplified production layout viewing of each locus of the application)：
Be can be seen that from black line sign, the amplified production arrangement tension of DYS460, DYS389II in patent CN103866019
It is close, following two problems can be caused：
Problem 1), allelic ladder (ladder) parting mistake.
Fig. 3 shows dividing for the allelic ladder (ladder) obtained using patent CN103866019 systems
Type figure, it can be seen that shown patent CN103866019, the allelic ladder of locus DYS438 and DYS389II
Parting misplaces, and first peak of DYS438 should be 7, and red collimation mark is shown, first peak recognizes mistake, because the mistake causes subsequently
All dislocation.Allelic ladder parting mistake as well known to those skilled in the art will necessarily lead to not carry out sample
Correct parting.
Fig. 4 shows the parting figure of the allelic ladder (ladder) obtained using the application system, can be with
Find out, arranged between each allele site uniform, and primer specificity is high, can obtain complete STR partings, peak type is sharp
Clearly, balance is good.
Problem 2), should be previous locus parting, but in the range of next locus, it is impossible to interpretation.
The allele distributions of such as DYS460 are 7,8,9,10,11,12,13 (https://yhrd.org/tools/
Marker/DYS460, YHRD database).Our the locus DYS460 to 812 male's samples are tested, and are found wherein
The parting for having 4 samples is 13, and it is 14 to have two samples, as shown in the table.
LOCUS:DYS460
| Allelic gene typing | Number of samples | Frequency |
| 8 | 1 | 0.00123152709359606 |
| 9 | 195 | 0.240147783251232 |
| 10 | 328 | 0.403940886699507 |
| 11 | 268 | 0.330049261083744 |
| 12 | 14 | 0.0172413793103448 |
| 13 | 4 | 0.00492610837438424 |
| 14 | 2 | 0.00246305418719212 |
GD:0.67155324454175
For patent CN103866019, when it is 13 that parting occurs in DYS460, the position at correspondence peak is 120bp, 14 correspondences
124bp.And the range size of DYS458 is 121bp-164bp, so with the system of patent CN103866019, DYS460 equipotentials
Gene 14 can be in the range of DYS458, and DYS458 two peaks occurs and cannot accurately judge.DYS460 partings are 14 sample
Can be in the position of red frame shown in Fig. 5.Therefore, for some allelic gene types of DYS460 locus, patent CN103866019
System cannot accurately carry out parting.And in application scheme, because applicant is to the primer of each locus allele, with
And the combination and arrangement of specific locus are redesigned so that in the absence of above mentioned problem, by each etc. of above the application
The amplified production magnitude range of position gene understands that what DYS460 allele 14 will not be wrong appears in next locus
On the position of DYS389I.
2. detecting system of the present invention compares with patent CN103866019 detecting system stability
, by verification experimental verification, detecting system of the invention is in primer pond (primer mix) and PCR buffer solutions mixing one for applicant
The allele for detecting each locus that can stablize within week, and CN103866019 detecting systems are in primer pond and PCR
After buffer solution mixing, at least for the corresponding amplified production of DYS389II locus can because dimer is formed between primer and by
Gradually reduce, DYS389II locus can occur allelic gene typing missing because its amplified production is less than detection limit within two hours,
As shown in the table, allelic gene typing success rates are constantly reduced with time lengthening, and parting basically can not be carried out after one day,
And the application system remains able to carry out successfully all allele parting after placing one week, said system PCR system
Using conventional enzyme and reagent：
For locus DYS389II：
Detecting system of the present invention compared with patent CN103866019 detecting systems, with detection stability high, Neng Gougeng
It is the parting of accurate, complete acquisition each allele of male individual locus, family can be carried out to male individual for follow-up
Male's composition in the detection of male's composition and multiple male's mixing sample in investigation, patriarchy paternity identification, men and women's mixing sample
Detection provides accurate, complete data.
Sequence table
<110>Material Evidence Identification Center, Ministry of Public Security
<120>A kind of method and system for carrying out Y-STR partings to male individual using 26 Y-STR locus
<130> 165669GF
<160> 46
<170> PatentIn version 3.5
<210> 1
<211> 18
<212> DNA
<213>Artificial sequence
<220>
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<400> 1
cgaggaatct gacacctc 18
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<400> 2
gcattccata tcatctatc 19
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<211> 20
<212> DNA
<213>Artificial sequence
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atcttctgta tccaactctc 20
<210> 4
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<212> DNA
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<220>
<223>Primer
<400> 4
ggcttatccc tgagtagcag 20
<210> 5
<211> 21
<212> DNA
<213>Artificial sequence
<220>
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<400> 5
cgataatatt ttacacattt t 21
<210> 6
<211> 20
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cagggtgaca gtaaaatgaa 20
<210> 7
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<212> DNA
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<220>
<223>Primer
<400> 7
aatcagaccc agttgatgca a 21
<210> 8
<211> 19
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<220>
<223>Primer
<400> 8
agacgctcca aaggaccca 19
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<211> 19
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<220>
<223>Primer
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aactcagcaa caggaatga 19
<210> 10
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ctatggttct ggcattacaa g 21
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gactatgggc gtgagtgcat 20
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aaacagagga agaccctgtc attc 24
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<220>
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<400> 13
ctgaagagct agacaccat 19
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<220>
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<400> 14
atcgcattcc aattacatag t 21
<210> 15
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<212> DNA
<213>Artificial sequence
<220>
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<400> 15
ttagcacttt cagcacatca c 21
<210> 16
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<212> DNA
<213>Artificial sequence
<220>
<223>Primer
<400> 16
gactctttcc tctgatggtg 20
<210> 17
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<212> DNA
<213>Artificial sequence
<220>
<223>Primer
<400> 17
gcccatccaa atcattcata at 22
<210> 18
<211> 20
<212> DNA
<213>Artificial sequence
<220>
<223>Primer
<400> 18
agccggtgct ggaagacaga 20
<210> 19
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<220>
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<400> 19
atcgttggga ccttgtgata atg 23
<210> 20
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attagggttc tctagaggga cag 23
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gctgttccct attcattcaa tcat 24
<210> 22
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ctatagggag acggaataaa a 21
<210> 23
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<220>
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attcgtgtta tctctgcctt 20
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tgactggtgt gtcacagcat g 21
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agtccatgac agctgatgc 19
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<220>
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gtggcagacg cctataatcc 20
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tgacgttgtc aaagagcttc aa 22
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tttattccgc tgtgttggag a 21
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gctggagtca ttcctaatgt gg 22
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<220>
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aaactttgag gtatgtctca ta 22
<210> 31
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<220>
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ctgtcatgga atgctctctt g 21
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<213>Artificial sequence
<220>
<223>Primer
<400> 32
aatctgccca aatatcca 18
<210> 33
<211> 18
<212> DNA
<213>Artificial sequence
<220>
<223>Primer
<400> 33
atgctcgagt tgttatgg 18
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<212> DNA
<213>Artificial sequence
<220>
<223>Primer
<400> 34
ctgaagtggc ttggaattc 19
<210> 35
<211> 22
<212> DNA
<213>Artificial sequence
<220>
<223>Primer
<400> 35
ctagcgtcaa tctctgcacc tg 22
<210> 36
<211> 21
<212> DNA
<213>Artificial sequence
<220>
<223>Primer
<400> 36
ctgaatgact actgagtttc t 21
<210> 37
<211> 23
<212> DNA
<213>Artificial sequence
<220>
<223>Primer
<400> 37
atgtgtatga aaggtgtgaa cca 23
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<220>
<223>Primer
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tgtacggctg tctaagggat c 21
<210> 39
<211> 19
<212> DNA
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<220>
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<400> 39
gtcgttatcg caaacatag 19
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<213>Artificial sequence
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gatgcattct aggaagatta 20
<210> 41
<211> 21
<212> DNA
<213>Artificial sequence
<220>
<223>Primer
<400> 41
tgtaggcagg ttttatggag g 21
<210> 42
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<212> DNA
<213>Artificial sequence
<220>
<223>Primer
<400> 42
agtccgggag tgatagcatt ag 22
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<212> DNA
<213>Artificial sequence
<220>
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<400> 43
ccaaattcca gcttattatt cc 22
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<212> DNA
<213>Artificial sequence
<220>
<223>Primer
<400> 44
gcagagagta gatatagatg ataga 25
<210> 45
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<213>Artificial sequence
<220>
<223>Primer
<400> 45
ctgtctattc atctaacatc tttgtcatc 29
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<212> DNA
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gagatcacca atgaaatgta tttattc 27
Claims (8)
1. a kind of method that Y-STR partings are carried out to male individual using 26 Y-STR locus, it is characterised in that the method
Including：
1) DNA of the male individual is obtained；
2) described 26 genotype of Y-STR locus of DNA are obtained, 26 Y-STR locus are DYS19, DYS385a/
b、DYS389I/II、DYS390、DYS391、DYS392、DYS393、DYS437、DYS448、DYS456、DYS458、DYS635、
GATA H4, DYS438, DYS439, DYS460, DYS447, DYS527a/b, DYS617, DYS522, DYS444, DYS508 and
DYS533, including the step of use amplimer corresponding with the locus to expand to obtain amplified production it；
Wherein the amplimer of locus DYS460 corresponds to the nucleotide sequence of SEQ ID No.1 to SEQ ID No.2；Gene
The amplimer of seat DYS389I/II is identical, is the nucleotide sequence of SEQ ID No.3 to SEQ ID No.4；Locus
The amplimer of DYS390, DYS392, DYS458, DYS437 corresponds respectively to the core of SEQ ID No.5 to SEQ ID No.12
Nucleotide sequence；The amplimer of locus DYS385a/b is identical, is the nucleotides sequence of SEQ ID No.13 to SEQ ID No.14
Row；Locus GATA-H4, DYS522, DYS456, DYS391, DYS447, DYS438, DYS448, DYS393, DYS635,
The amplimer of DYS439, DYS19, DYS444 corresponds respectively to the nucleotides sequence of SEQ ID No.15 to SEQ ID No.38
Row；The amplimer of locus DYS527a/b is identical, is the nucleotide sequence of SEQ ID No.39 to SEQ ID No.40；Base
Because of seat DYS617, the amplimer of DYS508, DYS533 corresponds respectively to the nucleosides of SEQ ID No.41 to SEQ ID No.46
Acid sequence；
3) genotype according to 26 locus of the male individual obtains the Y-STR genotyping results of the male individual.
2. method according to claim 1, it is characterised in that the primer is fluorescent dye primer.
3. method according to claim 1, it is characterised in that after wherein 2) being additionally included in acquisition amplified production, use something lost
Pass analyzer and analyze the amplified production, the step of with the genotype for obtaining 26 locus.
4. a kind of system that Y-STR partings are carried out to male individual using 26 Y-STR locus, it is characterised in that the system
System includes that DNA obtains system, compound detection system, deduction system；
The DNA obtains system is used to obtain the DNA of the male individual；
The compound detection system is used to obtain 26 genotype of Y-STR locus of the DNA, and is obtained according to the genotype
The individual Y-STR genotyping results are obtained,
26 Y-STR locus be DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392,
DYS393、DYS437、DYS448、DYS456、DYS458、DYS635、GATA H4、DYS438、DYS439、DYS460、
DYS447, DYS527a/b, DYS617, DYS522, DYS444, DYS508 and DYS533, obtain the gene of 26 locus
The process of type includes the step of using amplimer corresponding with the locus to expand to obtain amplified production it；
Wherein the amplimer of locus DYS460 corresponds to the nucleotide sequence of SEQ ID No.1 to SEQ ID No.2；Gene
The amplimer of seat DYS389I/II is identical, is the nucleotide sequence of SEQ ID No.3 to SEQ ID No.4；Locus
The amplimer of DYS390, DYS392, DYS458, DYS437 corresponds respectively to the core of SEQ ID No.5 to SEQ ID No.12
Nucleotide sequence；The amplimer of locus DYS385a/b is identical, is the nucleotides sequence of SEQ ID No.13 to SEQ ID No.14
Row；Locus GATA-H4, DYS522, DYS456, DYS391, DYS447, DYS438, DYS448, DYS393, DYS635,
The amplimer of DYS439, DYS19, DYS444 corresponds respectively to the nucleotides sequence of SEQ ID No.15 to SEQ ID No.38
Row；The amplimer of locus DYS527a/b is identical, is the nucleotide sequence of SEQ ID No.39 to SEQ ID No.40；Base
Because of seat DYS617, the amplimer of DYS508, DYS533 corresponds respectively to the nucleosides of SEQ ID No.41 to SEQ ID No.46
Acid sequence；
The deduction system is used to carry out family investigation, patriarchy to the male individual according to the individual Y-STR genotyping results
Male's ingredient origin is inferred in paternity identification, men and women's mixing sample, and/or male's ingredient origin is inferred in man's man's mixing sample.
5. system according to claim 4, it is characterised in that the primer is fluorescent dye primer.
6. system according to claim 5, it is characterised in that the compound detection system is additionally operable to obtaining amplified production
Afterwards, the amplified production is analyzed using genetic analyzer, to obtain the genotype of 27 locus.
7. a kind of compound detection system, it is characterised in that the system includes：Male individual DNA, 27 locus, amplification is drawn
Thing,
The compound detection system is used to be obtained using the amplimer amplified production of the DNA26 Y-STR locus,
And 26 genotype of locus of the DNA of male individual are obtained by the amplified production, and further obtained by the genotype
The individual Y-STR genotyping results,
26 Y-STR locus be DYS19, DYS385a/b, DYS389I/II, DYS390, DYS391, DYS392,
DYS393、DYS437、DYS448、DYS456、DYS458、DYS635、GATA H4、DYS438、DYS439、DYS460、
DYS447, DYS527a/b, DYS617, DYS522, DYS444, DYS508 and DYS533,
The amplimer is made up of amplimer corresponding with 26 locus, and the amplification of wherein locus DYS460 is drawn
Thing corresponds to the nucleotide sequence of SEQ ID No.1 to SEQ ID No.2；The amplimer of locus DYS389I/II is identical,
It is the nucleotide sequence of SEQ ID No.3 to SEQ ID No.4；The expansion of locus DYS390, DYS392, DYS458, DYS437
Increase the nucleotide sequence that primer corresponds respectively to SEQ ID No.5 to SEQ ID No.12；The amplification of locus DYS385a/b is drawn
Thing is identical, is the nucleotide sequence of SEQ ID No.13 to SEQ ID No.14；Locus GATA-H4, DYS522, DYS456,
The amplimer of DYS391, DYS447, DYS438, DYS448, DYS393, DYS635, DYS439, DYS19, DYS444 is right respectively
Should be in the nucleotide sequence of SEQ ID No.15 to SEQ ID No.38；The amplimer of locus DYS527a/b is identical, is
The nucleotide sequence of SEQ ID No.39 to SEQ ID No.40；The amplimer of locus DYS617, DYS508, DYS533 point
Not Dui Yingyu SEQ ID No.41 to SEQ ID No.46 nucleotide sequence.
8. a kind of detection kit, it is characterised in that including the compound detection system described in claim 7.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109136385A (en) * | 2017-10-20 | 2019-01-04 | 江苏苏博生物医学科技南京有限公司 | A kind of fluorescent marker detection kit for the Y-STR locus improving individual identification rate |
| CN110846420A (en) * | 2019-11-29 | 2020-02-28 | 河北医科大学 | Rapid mutation Y chromosome STR typing system, next generation sequencing typing kit, typing method and application |
| CN111621499A (en) * | 2020-05-19 | 2020-09-04 | 公安部物证鉴定中心 | Complex amplification detection kit for 17 autosomal STR and 28Y chromosome STR loci of human and application thereof |
| CN112176067A (en) * | 2019-07-02 | 2021-01-05 | 公安部物证鉴定中心 | Composite amplification system based on Y-STR locus and Y-Indels locus and primer combination used by same |
| CN112176068A (en) * | 2019-07-02 | 2021-01-05 | 公安部物证鉴定中心 | Composite amplification system based on 29Y-STR loci and primer combination used by same |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103866019A (en) * | 2014-03-11 | 2014-06-18 | 公安部物证鉴定中心 | Y-STR (Short Tandem Repeat) fluorescent compound amplification and detection reagent |
-
2016
- 2016-11-30 CN CN201611082622.9A patent/CN106755340B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103866019A (en) * | 2014-03-11 | 2014-06-18 | 公安部物证鉴定中心 | Y-STR (Short Tandem Repeat) fluorescent compound amplification and detection reagent |
Non-Patent Citations (1)
| Title |
|---|
| MANFRED KAYSER等: "A Comprehensive Survey of Human Y-Chromosomal Microsatellites", 《THE AMERICAN SOCIETY OF HUMAN GENETICS》 * |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109136385A (en) * | 2017-10-20 | 2019-01-04 | 江苏苏博生物医学科技南京有限公司 | A kind of fluorescent marker detection kit for the Y-STR locus improving individual identification rate |
| CN112176067A (en) * | 2019-07-02 | 2021-01-05 | 公安部物证鉴定中心 | Composite amplification system based on Y-STR locus and Y-Indels locus and primer combination used by same |
| CN112176068A (en) * | 2019-07-02 | 2021-01-05 | 公安部物证鉴定中心 | Composite amplification system based on 29Y-STR loci and primer combination used by same |
| CN112176068B (en) * | 2019-07-02 | 2022-08-02 | 公安部物证鉴定中心 | Composite amplification system based on 29Y-STR loci and primer combination used by same |
| CN112176067B (en) * | 2019-07-02 | 2022-12-02 | 公安部物证鉴定中心 | Composite amplification system based on Y-STR locus and Y-Indels locus and primer combination used by same |
| CN110846420A (en) * | 2019-11-29 | 2020-02-28 | 河北医科大学 | Rapid mutation Y chromosome STR typing system, next generation sequencing typing kit, typing method and application |
| CN110846420B (en) * | 2019-11-29 | 2023-06-02 | 河北医科大学 | Rapid mutation Y chromosome STR typing system, next generation sequencing typing kit, typing method and application |
| CN111621499A (en) * | 2020-05-19 | 2020-09-04 | 公安部物证鉴定中心 | Complex amplification detection kit for 17 autosomal STR and 28Y chromosome STR loci of human and application thereof |
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|---|---|
| CN106755340B (en) | 2020-07-07 |
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