CN106755331A - The detection method of Salmonella content in a kind of food - Google Patents
The detection method of Salmonella content in a kind of food Download PDFInfo
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- CN106755331A CN106755331A CN201611069291.5A CN201611069291A CN106755331A CN 106755331 A CN106755331 A CN 106755331A CN 201611069291 A CN201611069291 A CN 201611069291A CN 106755331 A CN106755331 A CN 106755331A
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- food
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
Abstract
The invention discloses a kind of detection method of Salmonella content in food, comprise the following steps：Step one, sample collection：Food 10g is gathered with the sampling bottle of advance sterilizing, is immediately placed in after food collection in ice chest and preserved, detected in 2 5h；Step 2, the concentration of food：The food low for salmonella concentration, vacuum filtration 1000mL foods take out filter membrane by miillpore filter, filtering after finishing, it is placed in sterilized beaker, 10mL phosphate buffers are added, the pH value of the phosphate buffer is 7.4, and filter membrane 30min is eluted with magnetic agitation.The present invention can salmonella living body content in quantitative determination food, and improve the specificity of detection, it is easy to operate feasible.
Description
Technical field
The present invention relates to a kind of detection method, the detection method of Salmonella content in specifically a kind of food.
Background technology
For the detection of salmonella, cultivation, immunological detection and the class of nucleic acid detection method three can be divided into.Cultivation
The growth characteristics according to salmonella are typically with immunological detection, salmonella is turned into excellent using the culture medium of selectivity
Gesture flora, is then based on the biochemical characteristic and somatic surface antigen characteristic of salmonella, anti-using biochemical reaction and Ag-Ab
The specificity answered carries out the detection of salmonella.Mostly there is complex operation, be easily disturbed, be difficult to quantitative determination in these methods
Problem.Nucleic acid detection method is the genetic fragment that salmonella is guarded to be detected using round pcr realize accurate detection
Purpose, however its it is maximum the drawbacks of be the thalline that cannot be distinguished by live body and dead, it is simple to be furtherd investigate using round pcr
Influence of the salmonella to health in food.
The content of the invention
It is an object of the invention to provide a kind of detection method of Salmonella content in food, to solve above-mentioned background
The problem proposed in technology.
To achieve the above object, the present invention provides following technical scheme：
The detection method of Salmonella content, comprises the following steps in a kind of food：Step one, sample collection：With going out in advance
The sampling bottle collection food 10g of bacterium, is immediately placed in ice chest after food collection and preserves, and is detected in 2-5h；Step 2, food
Concentration：The food low for salmonella concentration, vacuum filtration 1000mL foods by miillpore filter, after finishing take out by filtering
Filter membrane, is placed in sterilized beaker, adds 10mL phosphate buffers, and the pH value of the phosphate buffer is 7.4, uses magnetic force
Stirring wash-out filter membrane 30min；Filter membrane is abandoned, eluted product is preserved, eluted product 1mL is taken, is added in 9mL ultra-pure waters, after mixing
1mL is further taken out, is added in 9mL ultra-pure waters, successively gradient dilution, obtain 10-0、10-1With 10-2Three kinds of samples of dilution factor；It is right
In salmonella concentration food high, raw water 1mL is taken, be added in 9mL ultra-pure waters, 1mL is further taken out after mixing, be added to 9mL
In ultra-pure water, gradient dilution, obtains 10 successively-0、10-1With 10-2The sample of dilution factor；Step 3, increases bacterium before food：By 10-0、
10-1With 10-2The sample of these three dilution factors is separately added into preceding enrichment liquid and mixes, and each dilution factor does 5 parts, is put into 37 DEG C of constant temperature
24h is cultivated in incubator；Step 4, selective enrichment：The preceding increasing bacterium product 1mL of each dilution factor sample is transferred to 100mL
In selective enrichment broth and mix, selective enrichment culture 24h is carried out at 42 DEG C；Step 5, plate streaking is separated：Picking is selected
Selecting property increases bacterium product, separates and cultivates in XLD flat boards and the flat lining outs of BS respectively, wherein, XLD flat boards are cultivated at 37 DEG C
24h, BS flat board cultivate 48h at 37 DEG C；Step 6, the identification of positive bacterium colony：Culture is finished, and is observed on XLD and BS flat boards
The colonial morphology of growth, the bacterium colony of picking different shape, is placed in PCR reaction tubes respectively, is entered with Salmonella universal primer
Row amplification, expanding fragment length is 284bp；And to PCR primer under 100V electrophoresis 10-30min, observe on 284bp positions
Whether there is bright amplified band to occur, if so, then the bacterium colony is positive salmonella；Described Salmonella universal primer
Including sense primer 139 and anti-sense primer 141；Connect Salmonella universal primer and positive bacterium colony in the XLD flat boards
Touch to form the culture apparatus through being inoculated with；Culture apparatus first time period is incubated at a temperature of higher than 40 DEG C；Observe the training
Support device；Step 7, the positive bacterium colony on identification two kinds of flat boards of XLD and BS, and complementary counting is carried out, for each dilution factor
Sample, as long as corresponding XLD flat boards and BS flat boards have one is positive flat board, then the dilution factor is the positive；Combined with the positive
Mode record the result of the positive bacterium colony of flat board, the corresponding MPN values of control MPN table search, divided by step 2 food it is dense
The multiple of contracting, obtains the salmonella living body content of 100mL foods.
As further scheme of the invention：Wherein described culture apparatus is provided as film culture apparatus, the film
Culture apparatus have be disposed therein in the form of being substantially dehydrated the nutriment culture medium, the first choice agent,
The first distinctiveness indicator system and the second distinctiveness indicator system.
As further scheme of the invention：The condition of described PCR amplifications is：（1）PCR reaction systems cumulative volume 25
μ L, wherein each composition is final concentration of：141 each 0.4 μm of ol/L of primer 139 and primer, 0.75UTaq enzymes, 0.8mmol/LdNTPs,
2.5mmol/LMgCl2；（2）Pcr amplification reaction is carried out in PCR instrument, and reaction condition is：95 DEG C of predegeneration 5min；95 DEG C,
30s, 65 DEG C, 30s, 72 DEG C, 30s carries out 30 circulations；Last 72 DEG C of extensions 4min.
Compared with prior art, the beneficial effects of the invention are as follows：The present invention salmonella can live in quantitative determination food
Body content, and the specificity of detection is improved, it is easy to operate feasible.
Specific embodiment
The technical scheme in the embodiment of the present invention is clearly and completely described below.
In the embodiment of the present invention, the detection method of Salmonella content, comprises the following steps in a kind of food：Step
One, sample collection：Food 10g is gathered with the sampling bottle of advance sterilizing, is immediately placed in after food collection in ice chest and preserved, in 2-5h
Detected；Step 2, the concentration of food：The food low for salmonella concentration, vacuum filtration 1000mL foods are by micro-
Hole filter membrane, filtering takes out filter membrane after finishing, and is placed in sterilized beaker, adds 10mL phosphate buffers, the phosphate to delay
The pH value of fliud flushing is 7.4, and filter membrane 30min is eluted with magnetic agitation；Filter membrane is abandoned, eluted product is preserved, eluted product 1mL is taken, plus
Enter in 9mL ultra-pure waters, 1mL is further taken out after mixing, be added in 9mL ultra-pure waters, successively gradient dilution, obtain 10-0、10-1With
10-2Three kinds of samples of dilution factor；The food high for salmonella concentration, takes raw water 1mL, is added in 9mL ultra-pure waters, mixes
After further take out 1mL, be added in 9mL ultra-pure waters, successively gradient dilution, obtain 10-0、10-1With 10-2The sample of dilution factor；Step
Three, bacterium is increased before food：By 10-0、10-1With 10-2The sample of these three dilution factors is separately added into preceding enrichment liquid and mixes, each dilution
Degree does 5 parts, is put into 37 DEG C of constant incubators and cultivates 24h；Step 4, selective enrichment：By the preceding increasing of each dilution factor sample
Bacterium product 1mL is transferred in 100mL selective enrichment broths and mixes, and selective enrichment culture 24h is carried out at 42 DEG C；Step
Five, plate streaking is separated：Picking selective enrichment product, separates and cultivates in XLD flat boards and the flat lining outs of BS respectively, its
In, XLD flat boards cultivate 24h at 37 DEG C, and BS flat boards cultivate 48h at 37 DEG C；Step 6, the identification of positive bacterium colony：Cultivate
Finish, observe the colonial morphology grown on XLD and BS flat boards, the bacterium colony of picking different shape, is placed in PCR reaction tubes respectively,
Expanded with Salmonella universal primer, expanding fragment length is 284bp；And to PCR primer under 100V electrophoresis 10-
Whether 30min, observation has bright amplified band to occur on 284bp positions, if so, then the bacterium colony is positive salmonella；
Described Salmonella universal primer includes sense primer 139 and anti-sense primer 141；Make Salmonella in the XLD flat boards
Pseudomonas universal primer and positive bacterium colony contact to form the culture apparatus through being inoculated with；Culture dress is incubated at a temperature of higher than 40 DEG C
Put first time period；Observe the culture apparatus；Step 7, the positive bacterium colony on identification two kinds of flat boards of XLD and BS, and carry out mutually
Mend and count, for the sample of each dilution factor, as long as corresponding XLD flat boards and BS flat boards have one is positive flat board, then this is dilute
Degree of releasing is the positive；The result of the positive bacterium colony of flat board, the corresponding MPN of control MPN table search are recorded in the way of positive combination
Value, divided by the multiple of the food concentration in step 2, obtains the salmonella living body content of 100mL foods.Wherein described culture
Device is provided as film culture apparatus, and the film culture apparatus has the institute being disposed therein in the form of being substantially dehydrated
Nutriment culture medium, the first choice agent, the first distinctiveness indicator system and second distinctiveness is stated to indicate
Agent system.The condition of described PCR amplifications is：（1）The μ L of PCR reaction systems cumulative volume 25, wherein each composition is final concentration of：Primer
139 and primer 141 each 0.4 μm of ol/L, 0.75UTaq enzymes, 0.8mmol/LdNTPs, 2.5mmol/LMgCl2；（2）PCR amplifications are anti-
Should be carried out in PCR instrument, reaction condition is：95 DEG C of predegeneration 5min；95 DEG C, 30s, 65 DEG C, 30s, 72 DEG C, 30s carries out 30
Circulation；Last 72 DEG C of extensions 4min.
Embodiment 1：
The detection method of Salmonella content, comprises the following steps in food of the present invention：Step one, sample collection：With advance
The sampling bottle collection food 10g of sterilizing, is immediately placed in ice chest after food collection and preserves, and is detected in 2h；Step 2, food
Concentration：The food low for salmonella concentration, vacuum filtration 1000mL foods by miillpore filter, after finishing take out by filtering
Filter membrane, is placed in sterilized beaker, adds 10mL phosphate buffers, and the pH value of the phosphate buffer is 7.4, uses magnetic force
Stirring wash-out filter membrane 30min；Filter membrane is abandoned, eluted product is preserved, eluted product 1mL is taken, is added in 9mL ultra-pure waters, after mixing
1mL is further taken out, is added in 9mL ultra-pure waters, successively gradient dilution, obtain 10-0、10-1With 10-2Three kinds of samples of dilution factor；It is right
In salmonella concentration food high, raw water 1mL is taken, be added in 9mL ultra-pure waters, 1mL is further taken out after mixing, be added to 9mL
In ultra-pure water, gradient dilution, obtains 10 successively-0、10-1With 10-2The sample of dilution factor；Step 3, increases bacterium before food：By 10-0、
10-1With 10-2The sample of these three dilution factors is separately added into preceding enrichment liquid and mixes, and each dilution factor does 5 parts, is put into 37 DEG C of constant temperature
24h is cultivated in incubator；Step 4, selective enrichment：The preceding increasing bacterium product 1mL of each dilution factor sample is transferred to 100mL
In selective enrichment broth and mix, selective enrichment culture 24h is carried out at 42 DEG C；Step 5, plate streaking is separated：Picking is selected
Selecting property increases bacterium product, separates and cultivates in XLD flat boards and the flat lining outs of BS respectively, wherein, XLD flat boards are cultivated at 37 DEG C
24h, BS flat board cultivate 48h at 37 DEG C；Step 6, the identification of positive bacterium colony：Culture is finished, and is observed on XLD and BS flat boards
The colonial morphology of growth, the bacterium colony of picking different shape, is placed in PCR reaction tubes respectively, is entered with Salmonella universal primer
Row amplification, expanding fragment length is 284bp；And to PCR primer under 100V electrophoresis 10-30min, observe on 284bp positions
Whether there is bright amplified band to occur, if so, then the bacterium colony is positive salmonella；Described Salmonella universal primer
Including sense primer 139 and anti-sense primer 141；Connect Salmonella universal primer and positive bacterium colony in the XLD flat boards
Touch to form the culture apparatus through being inoculated with；Culture apparatus first time period is incubated at a temperature of higher than 40 DEG C；Observe the training
Support device；Step 7, the positive bacterium colony on identification two kinds of flat boards of XLD and BS, and complementary counting is carried out, for each dilution factor
Sample, as long as corresponding XLD flat boards and BS flat boards have one is positive flat board, then the dilution factor is the positive；Combined with the positive
Mode record the result of the positive bacterium colony of flat board, the corresponding MPN values of control MPN table search, divided by step 2 food it is dense
The multiple of contracting, obtains the salmonella living body content of 100mL foods.Wherein described culture apparatus is provided as film culture dress
Put, the film culture apparatus has the nutriment culture medium, described being disposed therein in the form of being substantially dehydrated
First choice agent, the first distinctiveness indicator system and the second distinctiveness indicator system.Described PCR amplifications
Condition is：（1）The μ L of PCR reaction systems cumulative volume 25, wherein each composition is final concentration of：141 each 0.4 μm of ol/ of primer 139 and primer
L, 0.75UTaq enzyme, 0.8mmol/LdNTPs, 2.5mmol/LMgCl2；（2）Pcr amplification reaction is carried out in PCR instrument, reacts bar
Part is：95 DEG C of predegeneration 5min；95 DEG C, 30s, 65 DEG C, 30s, 72 DEG C, 30s carries out 30 circulations；Last 72 DEG C of extensions 4min.
Embodiment 2：
The detection method of Salmonella content, comprises the following steps in food of the present invention：Step one, sample collection：With advance
The sampling bottle collection food 10g of sterilizing, is immediately placed in ice chest after food collection and preserves, and is detected in 5h；Step 2, food
Concentration：The food low for salmonella concentration, vacuum filtration 1000mL foods by miillpore filter, after finishing take out by filtering
Filter membrane, is placed in sterilized beaker, adds 10mL phosphate buffers, and the pH value of the phosphate buffer is 7.4, uses magnetic force
Stirring wash-out filter membrane 30min；Filter membrane is abandoned, eluted product is preserved, eluted product 1mL is taken, is added in 9mL ultra-pure waters, after mixing
1mL is further taken out, is added in 9mL ultra-pure waters, successively gradient dilution, obtain 10-0、10-1With 10-2Three kinds of samples of dilution factor；It is right
In salmonella concentration food high, raw water 1mL is taken, be added in 9mL ultra-pure waters, 1mL is further taken out after mixing, be added to 9mL
In ultra-pure water, gradient dilution, obtains 10 successively-0、10-1With 10-2The sample of dilution factor；Step 3, increases bacterium before food：By 10-0、
10-1With 10-2The sample of these three dilution factors is separately added into preceding enrichment liquid and mixes, and each dilution factor does 5 parts, is put into 37 DEG C of constant temperature
24h is cultivated in incubator；Step 4, selective enrichment：The preceding increasing bacterium product 1mL of each dilution factor sample is transferred to 100mL
In selective enrichment broth and mix, selective enrichment culture 24h is carried out at 42 DEG C；Step 5, plate streaking is separated：Picking is selected
Selecting property increases bacterium product, separates and cultivates in XLD flat boards and the flat lining outs of BS respectively, wherein, XLD flat boards are cultivated at 37 DEG C
24h, BS flat board cultivate 48h at 37 DEG C；Step 6, the identification of positive bacterium colony：Culture is finished, and is observed on XLD and BS flat boards
The colonial morphology of growth, the bacterium colony of picking different shape, is placed in PCR reaction tubes respectively, is entered with Salmonella universal primer
Row amplification, expanding fragment length is 284bp；And to PCR primer under 100V electrophoresis 10-30min, observe on 284bp positions
Whether there is bright amplified band to occur, if so, then the bacterium colony is positive salmonella；Described Salmonella universal primer
Including sense primer 139 and anti-sense primer 141；Connect Salmonella universal primer and positive bacterium colony in the XLD flat boards
Touch to form the culture apparatus through being inoculated with；Culture apparatus first time period is incubated at a temperature of higher than 40 DEG C；Observe the training
Support device；Step 7, the positive bacterium colony on identification two kinds of flat boards of XLD and BS, and complementary counting is carried out, for each dilution factor
Sample, as long as corresponding XLD flat boards and BS flat boards have one is positive flat board, then the dilution factor is the positive；Combined with the positive
Mode record the result of the positive bacterium colony of flat board, the corresponding MPN values of control MPN table search, divided by step 2 food it is dense
The multiple of contracting, obtains the salmonella living body content of 100mL foods.Wherein described culture apparatus is provided as film culture dress
Put, the film culture apparatus has the nutriment culture medium, described being disposed therein in the form of being substantially dehydrated
First choice agent, the first distinctiveness indicator system and the second distinctiveness indicator system.Described PCR amplifications
Condition is：（1）The μ L of PCR reaction systems cumulative volume 25, wherein each composition is final concentration of：141 each 0.4 μm of ol/ of primer 139 and primer
L, 0.75UTaq enzyme, 0.8mmol/LdNTPs, 2.5mmol/LMgCl2；（2）Pcr amplification reaction is carried out in PCR instrument, reacts bar
Part is：95 DEG C of predegeneration 5min；95 DEG C, 30s, 65 DEG C, 30s, 72 DEG C, 30s carries out 30 circulations；Last 72 DEG C of extensions 4min.
It is obvious to a person skilled in the art that the invention is not restricted to the details of above-mentioned one exemplary embodiment, Er Qie
In the case of without departing substantially from spirit or essential attributes of the invention, the present invention can be in other specific forms realized.Therefore, no matter
From the point of view of which point, embodiment all should be regarded as exemplary, and be nonrestrictive, the scope of the present invention is by appended power
Profit requires to be limited rather than described above, it is intended that all in the implication and scope of the equivalency of claim by falling
Change is included in the present invention.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each implementation method is only wrapped
Containing an independent technical scheme, this narrating mode of specification is only that for clarity, those skilled in the art should
Specification an as entirety, the technical scheme in each embodiment can also be formed into those skilled in the art through appropriately combined
May be appreciated other embodiment.
Claims (3)
1. in a kind of food Salmonella content detection method, it is characterised in that comprise the following steps：Step one, sample
Collection：Food 10g is gathered with the sampling bottle of advance sterilizing, is immediately placed in after food collection in ice chest and preserved, examined in 2-5h
Survey；Step 2, the concentration of food：The food low for salmonella concentration, vacuum filtration 1000mL foods pass through miillpore filter,
Filtering takes out filter membrane after finishing, and is placed in sterilized beaker, adds 10mL phosphate buffers, the pH of the phosphate buffer
It is 7.4 to be worth, and filter membrane 30min is eluted with magnetic agitation；Filter membrane is abandoned, eluted product is preserved, eluted product 1mL is taken, 9mL is added to and is surpassed
In pure water, 1mL is further taken out after mixing, be added in 9mL ultra-pure waters, successively gradient dilution, obtained and three kinds of samples of dilution factor
Product；The food high for salmonella concentration, takes raw water 1mL, is added in 9mL ultra-pure waters, and 1mL is further taken out after mixing, adds
To in 9mL ultra-pure waters, gradient dilution, obtains 10 successively-0、10-1With 10-2The sample of dilution factor；Step 3, increases bacterium before food：Will
10-0、10-1With 10-2The sample of these three dilution factors is separately added into preceding enrichment liquid and mixes, and each dilution factor does 5 parts, is put into 37 DEG C
24h is cultivated in constant incubator；Step 4, selective enrichment：The preceding increasing bacterium product 1mL of each dilution factor sample is transferred to
In 100mL selective enrichment broths and mix, selective enrichment culture 24h is carried out at 42 DEG C；Step 5, plate streaking is separated：
Picking selective enrichment product, separates and cultivates in XLD flat boards and the flat lining outs of BS respectively, wherein, XLD flat boards are at 37 DEG C
Culture 24h, BS flat boards cultivate 48h at 37 DEG C；Step 6, the identification of positive bacterium colony：Culture is finished, and observation is flat in XLD and BS
The colonial morphology grown on plate, the bacterium colony of picking different shape, is placed in PCR reaction tubes respectively, draws so that Salmonella is general
Thing is expanded, and expanding fragment length is 284bp；And to PCR primer under 100V electrophoresis 10-30min, observe at 284bp
Whether have bright amplified band occur, if so, then the bacterium colony is positive salmonella if putting；Described Salmonella is general
Primer includes sense primer 139 and anti-sense primer 141；Make Salmonella universal primer and positive bacteria in the XLD flat boards
Fall contact to forming the culture apparatus through being inoculated with；Culture apparatus first time period is incubated at a temperature of higher than 40 DEG C；Observation institute
State culture apparatus；Step 7, the positive bacterium colony on identification two kinds of flat boards of XLD and BS, and complementary counting is carried out, for each dilution
The sample of degree, as long as corresponding XLD flat boards and BS flat boards have one is positive flat board, then the dilution factor is the positive；With the positive
The mode of combination records the result of the positive bacterium colony of flat board, and the corresponding MPN values of control MPN table search are eaten divided by step 2
The multiple of thing concentration, obtains the salmonella living body content of 100mL foods.
2. in food according to claim 1 Salmonella content detection method, it is characterised in that wherein described training
Foster device is provided as film culture apparatus, and the film culture apparatus has what is be disposed therein in the form of being substantially dehydrated
The nutriment culture medium, the first choice agent, the first distinctiveness indicator system and second distinctiveness refer to
Show agent system.
3. in food according to claim 1 Salmonella content detection method, it is characterised in that described PCR
The condition of amplification is：（1）The μ L of PCR reaction systems cumulative volume 25, wherein each composition is final concentration of：Primer 139 and primer 141 are each
0.4 μm of ol/L, 0.75UTaq enzymes, 0.8mmol/LdNTPs, 2.5mmol/LMgCl2；（2）Pcr amplification reaction is enterprising in PCR instrument
OK, reaction condition is：95 DEG C of predegeneration 5min；95 DEG C, 30s, 65 DEG C, 30s, 72 DEG C, 30s carries out 30 circulations；Last 72 DEG C
Extend 4min.
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| CN201611069291.5A CN106755331A (en) | 2016-11-29 | 2016-11-29 | The detection method of Salmonella content in a kind of food |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201611069291.5A CN106755331A (en) | 2016-11-29 | 2016-11-29 | The detection method of Salmonella content in a kind of food |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107918016A (en) * | 2017-09-19 | 2018-04-17 | 中华人民共和国龙口出入境检验检疫局 | A kind of method quantitatively detected to Salmonella in Food based on growth curve |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102154472A (en) * | 2011-01-18 | 2011-08-17 | 西安建筑科技大学 | Quantitative detection method of salmonella living body in water |
| CN104105795A (en) * | 2011-12-28 | 2014-10-15 | 3M创新有限公司 | Method of detecting a salmonella microorganism |
-
2016
- 2016-11-29 CN CN201611069291.5A patent/CN106755331A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102154472A (en) * | 2011-01-18 | 2011-08-17 | 西安建筑科技大学 | Quantitative detection method of salmonella living body in water |
| CN104105795A (en) * | 2011-12-28 | 2014-10-15 | 3M创新有限公司 | Method of detecting a salmonella microorganism |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107918016A (en) * | 2017-09-19 | 2018-04-17 | 中华人民共和国龙口出入境检验检疫局 | A kind of method quantitatively detected to Salmonella in Food based on growth curve |
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