CN106755187A - Transparent xanthan gum preparation method and the culture medium for preparing transparent xanthan gum - Google Patents
Transparent xanthan gum preparation method and the culture medium for preparing transparent xanthan gum Download PDFInfo
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- CN106755187A CN106755187A CN201710076896.5A CN201710076896A CN106755187A CN 106755187 A CN106755187 A CN 106755187A CN 201710076896 A CN201710076896 A CN 201710076896A CN 106755187 A CN106755187 A CN 106755187A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
- C12P19/06—Xanthan, i.e. Xanthomonas-type heteropolysaccharides
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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Abstract
Culture medium the invention discloses transparent xanthan gum preparation method and for preparing transparent xanthan gum；Wherein, transparent xanthan gum preparation method, it comprises the following steps：(1) dispensing；(2) sterilize；(3) ferment；(4) fermentation liquor pretreatment；(5) extract packaging and transparent xanthan gum finished product is obtained.Culture medium for preparing transparent xanthan gum, its component for including following weight percentage：Glucose 4 6%, soya-bean milk 4 7%, additive 0.08 0.35%, balance of water, each component percentage composition sum is 100%.In xanthans preparation technology of the present invention, cost of material is relatively low, and the xanthans light transmittance produced is high, disclosure satisfy that the use requirement of beverage or cosmetics.Transparent xanthan gum product prepared by the present invention, is compareed with the light transmittance of distilled water, content be 1% xanthan gum solution wavelength be 600nm when light transmittance be higher than 93%.
Description
Technical field
The invention belongs to xanthans production technical field, and in particular to transparent xanthan gum preparation method and transparent for preparing
The culture medium of xanthans.
Background technology
Xanthans superior performance, widely used, its consumption is concentrated mainly on the neck such as food, petroleum industry and commodity industry
Domain.Domestic about 45% xanthans is sold as food-grade, and 40% sells as grade oil, 15% be used for agricultural chemicals, feed,
The industries such as daily use chemicals, environmental protection.
The production technology of xanthans includes fermenting and extracts two stages.Fermentation stage includes that seed culture, fermentation are obtained
Xanthan gum fermentation broth, subsequently into packing stage is extracted, is separated out using alcohol precipitation, is dried, is packaged to be finished product.At present, make
The culture medium raw material that standby xanthans is used：Carbon source is starch, and nitrogen source is bean powder, the xanthans prepared with conventional method, after dissolving
It is faint yellow or milky opaque liquid, it is applied in part beverage or cosmetics, and translucency cannot meet requirement.
The content of the invention
First purpose of the invention is that a kind of transparency of offer is high, the relatively low transparent xanthan gum system of cost of material
Preparation Method.
Second object of the present invention is to provide the culture medium for preparing transparent xanthan gum.
First purpose of the invention is implemented by following technical scheme：Transparent xanthan gum preparation method, it includes following step
Suddenly：(1) dispensing；(2) sterilize；(3) ferment；(4) fermentation liquor pretreatment；(5) extract packaging and transparent xanthan gum finished product is obtained；
Step (1) dispensing：Dispensing as following weight percent：Glucose 4-6%, soya-bean milk 4-7%, additive
0.08-0.35%, balance of water, each component percentage composition sum is 100%；
Step (3) fermentation：To be fermented after mixed material inoculation fermentation bacterium after sterilizing, fermentation temperature is 27-
32 DEG C, the tank pressure of fermentation tank is 0.04-0.06MPa, and material pH value is 6.5-7.5 in fermentation process, and ferment 64-72h, and hair is obtained
Zymotic fluid；
Step (4) fermentation liquor pretreatment：
A, the zymotic fluid is warming up to 50-60 DEG C after, in the zymotic fluid add EDTA react 1-1.5 hour, make
The first fermentation pretreatment fluid is obtained, wherein, the addition of the EDTA is the 0.5-1% of the zymotic fluid quality；EDTA is added to use
Metal ion in zymotic fluid is complexed, it is to avoid in metal ion influence subsequent technique, the activity of lysozyme and protease.
Bacteriolyze enzyme reaction 1-2 hours is added in B, the pretreatment fluid that fermented to described first, reaction temperature is controlled in 50-60
DEG C, the second fermentation pretreatment fluid is obtained, wherein, the addition of the lysozyme is the 0.2- of the first fermentation pretreatment fluid
0.4‰；Lysozyme is added to cause the Bacterial cell lysis in zymotic fluid.
Mmp reaction is added in C, the pretreatment fluid that fermented to the described 3rd 1-2 hours, reaction temperature is controlled in 50-60
DEG C, fermentation pretreatment fluid is obtained, wherein, the addition of the protease is the 0.2-0.5 ‰ of the second fermentation pretreatment fluid.
Add the protein in proteases for decomposing zymotic fluid.
Specifically, the additive is for the defoamer and weight/mass percentage composition that weight/mass percentage composition is 0.02-0.05%
The calcium carbonate of 0.06-0.3%.
Preferably, prepare during the soya-bean milk, the mass ratio of soya bean and water is 1:10-1:15.
Preferably, the zymophyte is Xanthomonas campestris.
Second object of the present invention is implemented by following technical scheme：Culture medium for preparing transparent xanthan gum, its bag
Include the component of following weight percentage：Glucose 4-6%, soya-bean milk 4-7%, additive 0.08-0.35%, balance of water, respectively
Component percentages sum is 100%.
Preferably, prepare during the soya-bean milk, the mass ratio of soya bean and water is 1:10-1:15.
Specifically, the additive is for the defoamer and weight/mass percentage composition that weight/mass percentage composition is 0.02-0.05%
The calcium carbonate of 0.06-0.3%.
Ethylenediamine tetra-acetic acid (EDTA) is a kind of organic compound, is white powder under normal temperature and pressure.It is that one kind can be with
Mg2+、Ca2+、Mn2+、Fe2+Deng the chelating agent that bivalent metal ion is combined.Due to most nucleic acid enzymes and the work of some proteases
With needing Mg2+, therefore the conventional inhibitor for doing nuclease, protease；Can also be used for inhibitory action of the heavy-metal ion removal to enzyme.
The advantage of the invention is that：In xanthans preparation technology of the present invention, cost of material is relatively low, and the Huang produced
Virgin rubber light transmittance is high, disclosure satisfy that the use requirement of beverage or cosmetics.The xanthans prepared with existing convenient source and method
Product, is compareed with the light transmittance of distilled water, content be 1% xanthan gum solution wavelength be 600nm when light transmittance one
As be 40% or so, and the transparent xanthan gum product prepared by the present invention is compareed with the light transmittance of distilled water, and content is 1%
Xanthan gum solution wavelength be 600nm when light transmittance be higher than 93%.
Specific embodiment
With reference to specific embodiment, the present invention is described in detail, but following examples are not constituted to of the invention
Limitation.
Embodiment 1：Culture medium for preparing transparent xanthan gum, its component for including following weight percentage：Grape
Sugar 4%, soya-bean milk 4%, defoamer 0.02%, calcium carbonate 0.06%, balance of water, each component percentage composition sum is 100%.Its
In, preparing during the soya-bean milk, the mass ratio of soya bean and water is 1:10.
Ethylenediamine tetra-acetic acid (EDTA) is a kind of organic compound, is white powder under normal temperature and pressure.It is that one kind can be with
Mg2+、Ca2+、Mn2+、Fe2+Deng the chelating agent that bivalent metal ion is combined.Due to most nucleic acid enzymes and the work of some proteases
With needing Mg2+, therefore the conventional inhibitor for doing nuclease, protease；Can also be used for inhibitory action of the heavy-metal ion removal to enzyme.
Embodiment 2：Transparent xanthan gum preparation method, it comprises the following steps：(1) dispensing；(2) sterilize；(3) ferment；(4)
Fermentation liquor pretreatment；(5) extract packaging and transparent xanthan gum finished product is obtained；
Step (1) dispensing：Dispensing as following weight percent：Glucose 4%, soya-bean milk 4%, defoamer 0.02%,
Calcium carbonate 0.06%, balance of water, each component percentage composition sum is 100%.Wherein, during preparing the soya-bean milk, soya bean
It is 1 with the mass ratio of water:10.
Step (3) fermentation：To be fermented after mixed material inoculation Xanthomonas campestris after sterilizing, fermentation temperature is
27 DEG C, the tank pressure of fermentation tank is 0.04MPa, and material pH value is 6.5 in fermentation process, and ferment 64h, and zymotic fluid is obtained；
Step (4) fermentation liquor pretreatment：
A, the zymotic fluid is warming up to 50 DEG C after, in the zymotic fluid add EDTA react 1 hour, be obtained first hair
Ferment pretreatment fluid, wherein, the addition of the EDTA is the 0.5% of the zymotic fluid quality；Adding EDTA is used to be complexed fermentation
Metal ion in liquid, it is to avoid in metal ion influence subsequent technique, the activity of lysozyme and protease.
Bacteriolyze enzyme reaction 1 hour is added in B, the pretreatment fluid that fermented to described first, reaction temperature is controlled at 50 DEG C, is obtained
Second fermentation pretreatment fluid, wherein, the addition of the lysozyme is the 0.2 ‰ of the first fermentation pretreatment fluid；Add molten
Bacterium enzyme causes the Bacterial cell lysis in zymotic fluid.
Mmp reaction is added in C, the pretreatment fluid that fermented to the described 3rd 1 hour, reaction temperature is controlled at 50 DEG C, is obtained
Fermentation pretreatment fluid, wherein, the addition of the protease is the 0.2 ‰ of the second fermentation pretreatment fluid.Add protease
Protein in decomposing and fermenting liquid.
Transparent xanthan gum product manufactured in the present embodiment, is compareed with the light transmittance of distilled water, and content is 1% xanthans
Light transmittance of the aqueous solution when wavelength is 600nm is 93.6%.
Embodiment 3：Culture medium for preparing transparent xanthan gum, its component for including following weight percentage：Grape
Sugar 5%, soya-bean milk 6%, defoamer 0.03%, calcium carbonate 0.15%, balance of water, each component percentage composition sum is 100%.Its
In, preparing during the soya-bean milk, the mass ratio of soya bean and water is 1:12.
Ethylenediamine tetra-acetic acid (EDTA) is a kind of organic compound, is white powder under normal temperature and pressure.It is that one kind can be with
Mg2+、Ca2+、Mn2+、Fe2+Deng the chelating agent that bivalent metal ion is combined.Due to most nucleic acid enzymes and the work of some proteases
With needing Mg2+, therefore the conventional inhibitor for doing nuclease, protease；Can also be used for inhibitory action of the heavy-metal ion removal to enzyme.
Embodiment 4：Transparent xanthan gum preparation method, it comprises the following steps：(1) dispensing；(2) sterilize；(3) ferment；(4)
Fermentation liquor pretreatment；(5) extract packaging and transparent xanthan gum finished product is obtained；
Step (1) dispensing：Dispensing as following weight percent：Glucose 5%, soya-bean milk 6%, defoamer 0.03%,
Calcium carbonate 0.15%, balance of water, each component percentage composition sum is 100%.Wherein, during preparing the soya-bean milk, soya bean
It is 1 with the mass ratio of water:12；
Step (3) fermentation：To be fermented after mixed material inoculation Xanthomonas campestris after sterilizing, fermentation temperature is
30 DEG C, the tank pressure of fermentation tank is 0.05MPa, and material pH value is 7 in fermentation process, and ferment 68h, and zymotic fluid is obtained；
Step (4) fermentation liquor pretreatment：
A, the zymotic fluid is warming up to 55 DEG C after, in the zymotic fluid add EDTA react 1.2 hours, be obtained first
Fermentation pretreatment fluid, wherein, the addition of the EDTA is the 0.8% of the zymotic fluid quality；Adding EDTA is used to be complexed hair
Metal ion in zymotic fluid, it is to avoid in metal ion influence subsequent technique, the activity of lysozyme and protease.
Bacteriolyze enzyme reaction 1.5 hours is added in B, the pretreatment fluid that fermented to described first, reaction temperature is controlled at 55 DEG C, system
The second fermentation pretreatment fluid is obtained, wherein, the addition of the lysozyme is the 0.3 ‰ of the first fermentation pretreatment fluid；Add
Lysozyme causes the Bacterial cell lysis in zymotic fluid.
Mmp reaction is added in C, the pretreatment fluid that fermented to the described 3rd 1.5 hours, reaction temperature is controlled at 55 DEG C, system
Must be fermented pretreatment fluid, wherein, the addition of the protease is the 0.35 ‰ of the second fermentation pretreatment fluid.Add albumen
Protein in enzyme decomposing and fermenting liquid.
Transparent xanthan gum product manufactured in the present embodiment, is compareed with the light transmittance of distilled water, and content is 1% xanthans
Light transmittance of the aqueous solution when wavelength is 600nm is 93.2%.
Embodiment 5：Culture medium for preparing transparent xanthan gum, its component for including following weight percentage：Grape
Sugar 6%, soya-bean milk 7%, defoamer 0.05%, calcium carbonate 0.3%, balance of water, each component percentage composition sum is 100%.Its
In, preparing during the soya-bean milk, the mass ratio of soya bean and water is 1:15.
Ethylenediamine tetra-acetic acid (EDTA) is a kind of organic compound, is white powder under normal temperature and pressure.It is that one kind can be with
Mg2+、Ca2+、Mn2+、Fe2+Deng the chelating agent that bivalent metal ion is combined.Due to most nucleic acid enzymes and the work of some proteases
With needing Mg2+, therefore the conventional inhibitor for doing nuclease, protease；Can also be used for inhibitory action of the heavy-metal ion removal to enzyme.
Embodiment 6：Transparent xanthan gum preparation method, it comprises the following steps：(1) dispensing；(2) sterilize；(3) ferment；(4)
Fermentation liquor pretreatment；(5) extract packaging and transparent xanthan gum finished product is obtained；
Step (1) dispensing：Dispensing as following weight percent：Glucose 6%, soya-bean milk 7%, defoamer 0.05%,
Calcium carbonate 0.3%, balance of water, each component percentage composition sum is 100%.Wherein, during preparing the soya-bean milk, soya bean and
The mass ratio of water is 1:15；
Step (3) fermentation：To be fermented after mixed material inoculation Xanthomonas campestris after sterilizing, fermentation temperature is
32 DEG C, the tank pressure of fermentation tank is 0.06MPa, and material pH value is 7.5 in fermentation process, and ferment 72h, and zymotic fluid is obtained；
Step (4) fermentation liquor pretreatment：
A, the zymotic fluid is warming up to 60 DEG C after, in the zymotic fluid add EDTA react 1.5 hours, be obtained first
Fermentation pretreatment fluid, wherein, the addition of the EDTA is the 1% of the zymotic fluid quality；Adding EDTA is used to be complexed fermentation
Metal ion in liquid, it is to avoid in metal ion influence subsequent technique, the activity of lysozyme and protease.
Bacteriolyze enzyme reaction 2 hours is added in B, the pretreatment fluid that fermented to described first, reaction temperature is controlled at 60 DEG C, is obtained
Second fermentation pretreatment fluid, wherein, the addition of the lysozyme is the 0.4 ‰ of the first fermentation pretreatment fluid；Add molten
Bacterium enzyme causes the Bacterial cell lysis in zymotic fluid.
Mmp reaction is added in C, the pretreatment fluid that fermented to the described 3rd 2 hours, reaction temperature is controlled at 60 DEG C, is obtained
Fermentation pretreatment fluid, wherein, the addition of the protease is the 0.5 ‰ of the second fermentation pretreatment fluid.Add protease
Protein in decomposing and fermenting liquid.
Transparent xanthan gum product manufactured in the present embodiment, is compareed with the light transmittance of distilled water, and content is 1% xanthans
Light transmittance of the aqueous solution when wavelength is 600nm is 93.9%.
Claims (7)
1. transparent xanthan gum preparation method, it comprises the following steps：(1) dispensing；(2) sterilize；(3) ferment；(4) zymotic fluid is located in advance
Reason；(5) extract packaging and transparent xanthan gum finished product is obtained；Characterized in that, step (1) dispensing：As following weight percent
Dispensing：Glucose 4-6%, soya-bean milk 4-7%, additive 0.08-0.35%, balance of water, each component percentage composition sum is
100%；
Step (3) fermentation：To be fermented after mixed material inoculation fermentation bacterium after sterilizing, fermentation temperature is 27-32 DEG C,
The tank pressure of fermentation tank is 0.04-0.06MPa, and material pH value is 6.5-7.5 in fermentation process, and ferment 64-72h, and zymotic fluid is obtained；
Step (4) fermentation liquor pretreatment：
A, the zymotic fluid is warming up to 50-60 DEG C after, to adding EDTA to react in the zymotic fluid 1-1.5 hour, prepared the
One fermentation pretreatment fluid, wherein, the addition of the EDTA is the 0.5-1% of the zymotic fluid quality；
Bacteriolyze enzyme reaction 1-2 hours is added in B, the pretreatment fluid that fermented to described first, reaction temperature is controlled at 50-60 DEG C, system
The second fermentation pretreatment fluid is obtained, wherein, the addition of the lysozyme is the 0.2-0.4 ‰ of the first fermentation pretreatment fluid；
Mmp reaction is added in C, the pretreatment fluid that fermented to the described 3rd 1-2 hours, reaction temperature is controlled at 50-60 DEG C, system
Must be fermented pretreatment fluid, wherein, the addition of the protease is the 0.2-0.5 ‰ of the second fermentation pretreatment fluid.
2. transparent xanthan gum preparation method according to claim 1, it is characterised in that the additive contains for quality percentage
Measure as the defoamer and weight/mass percentage composition of 0.02-0.05% are the calcium carbonate of 0.06-0.3%.
3. transparent xanthan gum preparation method according to claim 1, it is characterised in that yellow during preparing the soya-bean milk
The mass ratio of beans and water is 1:10-1:15.
4. transparent xanthan gum preparation method according to claim 1, it is characterised in that the zymophyte is yellow unit cell bar
Bacterium.
5. it is used to prepare the culture medium of transparent xanthan gum, it is characterised in that its component for including following weight percentage：Grape
Sugared 4-6%, soya-bean milk 4-7%, additive 0.08-0.35%, balance of water, each component percentage composition sum is 100%.
6. the culture medium for preparing transparent xanthan gum according to claim 5, it is characterised in that prepare the soya-bean milk mistake
The mass ratio of Cheng Zhong, soya bean and water is 1:10-1:15.
7. the culture medium for preparing transparent xanthan gum according to claim 5, it is characterised in that the additive is matter
Amount percentage composition is the calcium carbonate of 0.06-0.3% for the defoamer and weight/mass percentage composition of 0.02-0.05%.
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| CN201710076896.5A CN106755187A (en) | 2017-02-13 | 2017-02-13 | Transparent xanthan gum preparation method and the culture medium for preparing transparent xanthan gum |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111187796A (en) * | 2019-01-28 | 2020-05-22 | 健隆生物科技股份有限公司 | Xanthan gum fermentation medium prepared by using resistant dextrin byproduct and system |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6271001B1 (en) * | 1995-03-23 | 2001-08-07 | Bio Polymers Pty. Ltd. | Cultured plant cell gums for food, pharmaceutical, cosmetic and industrial applications |
| CN101460065A (en) * | 2006-12-15 | 2009-06-17 | Cp凯尔科美国股份有限公司 | Ultra high performance clarified gellan gums |
| CN102220394A (en) * | 2010-04-16 | 2011-10-19 | 淄博中轩生化有限公司 | Method for producing transparent xanthan gum |
-
2017
- 2017-02-13 CN CN201710076896.5A patent/CN106755187A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6271001B1 (en) * | 1995-03-23 | 2001-08-07 | Bio Polymers Pty. Ltd. | Cultured plant cell gums for food, pharmaceutical, cosmetic and industrial applications |
| CN101460065A (en) * | 2006-12-15 | 2009-06-17 | Cp凯尔科美国股份有限公司 | Ultra high performance clarified gellan gums |
| CN102220394A (en) * | 2010-04-16 | 2011-10-19 | 淄博中轩生化有限公司 | Method for producing transparent xanthan gum |
Non-Patent Citations (1)
| Title |
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| 赵兰坤等: "高透明黄原胶提取新工艺关键技术研究", 《发酵科技通讯》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111187796A (en) * | 2019-01-28 | 2020-05-22 | 健隆生物科技股份有限公司 | Xanthan gum fermentation medium prepared by using resistant dextrin byproduct and system |
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Application publication date: 20170531 |