CN106754504B - Stress-resistant assisted denitrification method for high-salinity nitrogen-containing wastewater - Google Patents
Stress-resistant assisted denitrification method for high-salinity nitrogen-containing wastewater Download PDFInfo
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Abstract
The invention discloses a stress-resistant assisted denitrification method for high-salinity nitrogen-containing wastewater, belonging to the technical field of microbial denitrification. The method provides a high-salinity nitrogen-containing wastewater denitrification technology by constructing a stress-resistant assisted synchronous heterotrophic nitrification aerobic denitrification mode combining tetrahydropyrimidine-secreting halomonas and a traditional denitrification strain. The invention solves the problem of low denitrification efficiency caused by the inhibition of high salt on the growth and metabolism of denitrification strains in the denitrification of the high-salt nitrogen-containing wastewater at present. Has application prospect in the aspects of high-salt nitrogen-containing wastewater treatment, mariculture water purification and the like.
Description
Technical Field
The invention belongs to the technical field of microbial denitrification, and particularly relates to a denitrification method based on microbial stress resistance assistance and used for synchronous heterotrophic nitrification and aerobic denitrification.
Background
Ammonia nitrogen is one of the main pollutants of water in China at present. The unreasonable discharge of ammonia nitrogen causes the ammonia nitrogen pollution of natural water bodies such as rivers, lakes, underground water, offshore areas and the like to become increasingly serious. Biological and environmental safety problems caused by ammonia nitrogen pollution are of high concern. The theory and the technical research of the ammonia nitrogen degradation and purification treatment of the sewage and the wastewater and the natural water body environment have great economic value and social benefit.
The technical methods used for purifying ammonia nitrogen mainly comprise physical and chemical methods such as a stripping method, a membrane absorption method, an MAP precipitation method, an ion exchange method, a breakpoint chlorine adding method and the like. After the bacterial strain with denitrification capability is discovered in the nineteenth century, the microbial degradation theory and technical research of ammonia nitrogen are rapidly developed, the full-process nitrification and denitrification, anaerobic ammonia oxidation and synchronous heterotrophic nitrification and aerobic denitrification processes are widely used for the denitrification of sewage and wastewater, and a systematic theoretical system for the biochemical process, denitrification related enzyme and gene research of denitrification is formed. The ammonia nitrogen microbial degradation technology embodies remarkable advantages in the aspect of advanced treatment of sewage and wastewater.
In recent years, a microbial ammonia nitrogen degradation technology of synchronous Heterotrophic nitrification-Aerobic Denitrification (SND) becomes a research hotspot. The SND denitrification is carried out synchronously in the same reactor and under the same operation condition by heterotrophic nitrification reaction and aerobic denitrification reaction. The SND denitrification technology has high denitrification efficiency; the pH value of the denitrification system is stable; is suitable for the denitrification treatment of the nitrogenous wastewater containing organic matters; simple process operation, reduced floor space, reduced cost, etc. The research of SND denitrification technology and theory is one of the research hotspots of the degradation of ammonia nitrogen microorganisms. Some of the SND denitrifying strains reported are Acinetobacter (Acinetobacter), Pseudomonas (Pseudomonas), Bacillus (Bacillus), Paracoccus (Paracoccus), Alcaligenes (Alcaligenes), etc. [ Suwanese, Jun Gaoshan, Zhao Hongmei, research progress of heterotrophic nitrification-aerobic denitrification bacteria ] Industrial Water treatment 2013.33(12):1-5 ].
Ammonia nitrogen wastewater in certain industries has the characteristics of high salt and high ammonia nitrogen, such as magnesium oxide desulfurization waste liquid of a marine diesel engine. The International Maritime Organization (IMO) established an agreement on MARPOL73/78 anti-fouling convention 1997 in 1997 according to the 15th principle of the declaration of the Environment and Productivity, and newly added a MARPOL73/78 anti-fouling convention Vl the rules of preventing atmospheric pollution caused by ships [ Zhou Waji, Zhang Dong ] implementing a countermeasure of MARPOL73/78 convention VI in this agreement.Marine technology, 2006, 6:43-44 ]. The regulations require that the total amount of sulfur oxide emissions from marine equipment be controlled to within 6.0 g/kwh. The marine magnesium oxide desulfurization process is a wet desulfurization technology which uses magnesium oxide as a desulfurizing agent and absorbs sulfur dioxide to generate hydrous magnesium sulfite and a small amount of magnesium sulfate slurry. The magnesium oxide wet fuel desulfurization waste liquid of the marine diesel engine mainly contains suspended matters, nitrates, nitrites, oxysulfides, organic pollutants, benzene series, straight-chain hydrocarbons, heavy metal compounds and the like, and has the characteristics of high salt content, high chemical oxygen demand, poor biodegradability and the like. IMO has relevant and specified emission standards for the pH value, the polycyclic aromatic hydrocarbon value, the turbidity, the nitrate content, additives and the like of the ship flue gas washing water. Except the waste liquid of wet fuel oil desulfurization of magnesium oxide of a marine diesel engine, the waste water of industries such as leather processing, marine product cultivation, synthetic ammonia, chemical fertilizer production, refuse landfill, nickel battery production and the like has the characteristics of high salt and high-concentration ammonia nitrogen.
The existing SND denitrification technology has low denitrification efficiency in the wastewater treatment. The main reason is that the growth and metabolic activity of the traditional non-salt-tolerant SND denitrification strain (such as Acinetobacter calcoaceticus, Pseudomonas marinus) is inhibited by high osmotic pressure caused by high salt or high-concentration ammonia nitrogen; secondly, intermediate nitrite with higher concentration is accumulated in the denitrification process of the high-concentration ammonia nitrogen water body, and nitrite with certain concentration has toxicity to cells, so that denitrification reaction is inhibited, and denitrification rate is reduced; third, some halophilic moderately halophilic bacteria (e.g., halonas campisalis ha3) with SND denitrification capability can tolerate a certain concentration of NaCl, but have a low reaction rate in the nitration process, and thus have a low SND denitrification rate [ Guo, y., Zhou, x.m., Li, y.g., Li, k.ang, Wang, c.x., Liu, j.f., Yan, d.j., Liu, y.l., Yang, D.H. & Xing, j.m. heterophilic and anodic Densition by a novel halonas biotechnologies letters.2013.35,2045-2049 ]. In addition, The species of organic carbon sources available for denitrification by bacteria of The genus Halomonas have certain limitations for practical applications [ Haba, R.R., Arahal, D.R., Sanchez-Porro, C. & Ventosa, A.2014 The prokaryotes: Gamma aproobacteria: 17 The family Halomonadaceae.Springer, Heidelberg,2014, 338-. The nitration reaction in the SND denitrification process is particularly sensitive to reaction conditions. A large number of researches show that the salt concentration is 10g/L NaCl, the nitration reaction starts to be inhibited, the nitration rate starts to be obviously reduced at 30g/L NaCl, and 60g/L NaCl is the limit concentration (Xuhanli, roof tree Wei, Ma Wei, Wangbiang, Wuweixiang) which can be tolerated by the nitration reaction system, and the influence and the mechanism of the salt on the nitration reaction in the biological denitrification process apply the ecology newspaper, 2014, 25(7), 2132-. In conclusion, the technical method for improving the SND denitrification efficiency of the high-salt nitrogen-containing wastewater has great economic value and social benefit.
Disclosure of Invention
The invention discloses a stress-resistant assisted denitrification method for high-salinity nitrogen-containing wastewater, belonging to the technical field of microbial denitrification. The method provides a high-salinity nitrogen-containing wastewater denitrification technology by constructing a stress-resistant assisted synchronous heterotrophic nitrification aerobic denitrification mode combining tetrahydropyrimidine-secreting halomonas and a traditional denitrification strain. The invention solves the problem of low denitrification efficiency caused by the inhibition of high salt on the growth and metabolism of denitrification strains in the denitrification of the high-salt nitrogen-containing wastewater at present. Has application prospect in the aspects of high-salt nitrogen-containing wastewater treatment, mariculture water purification and the like.
One aspect of the invention is: disclosed is an anti-adversity assisting function of a tetrahydropyrimidine secreting strain in SND denitrification. It comprises tetrahydropyrimidine secreting strain and SND denitrifying strain.
In the above technical scheme, the preferable tetrahydropyrimidine secreting strain is a strain with high tetrahydropyrimidine synthesis and secretion, for example, Halomonas venusta. The SND denitrifying strain is selected from Acinetobacter calcoaceticus, Pseudomonas marini, Paracoccus denitrificans, Alcaligenes faecalis, Bacillus cereus, Streptococcus chelidonii and Rhodococcus pyridinivorans.
When one strain is preferably selected from the tetrahydropyrimidine secreting strain and the SND denitrifying strain, the preferable inoculation proportion is 1: 0.5-2 by weight; more preferably, the inoculation ratio is 1: 1.
When the tetrahydropyrimidine-secreting strain is preferably one strain and the SND deaza strain is preferably two or more strains, the most preferable inoculation ratio is equal ratio by weight.
More specific preferred combinations are: halomonas: acinetobacter calcoaceticus: the inoculation ratio of the pseudomonas marinum is 1:1:1 by weight.
For the above technical solution, it is further preferred that the combined bacterial flora capable of SND denitrification while efficiently synthesizing and secreting tetrahydropyrimidine: halomonas venusta DSM4743, Acinetobacter calcoaceticus CCTCC AB 205304, and Pseudomonas marini MCCC1A 02281.
Another aspect is to provide a SND denitrification stress-resistance-assisting method for denitrifying a tetrahydropyrimidine-secreting strain in combination with a conventional SND denitrifying strain and using it for SND denitrification of high-salt nitrogen-containing wastewater.
The combined flora is applied to the technical fields of treating high-salt nitrogen-containing wastewater and purifying mariculture water bodies.
For the above technical scheme, the specific application can adopt a mode of simultaneous inoculation or staggered inoculation when treating high-salt nitrogen-containing wastewater and purifying a mariculture water body; under the preferable condition, the staggered inoculation mode of inoculating the ectoine secretory strain and then inoculating the SND denitrification strain is more beneficial to the growth of thalli and can effectively improve the denitrification rate. The specific interval time of the staggered inoculation is 6-18 h.
The high-salt nitrogen-containing wastewater refers to wastewater containing NaCl with the concentration more than or equal to 30g/L and nitrogen element with the concentration less than or equal to 3000 mg/L.
The invention has the innovative characteristics that:
① the invention provides a combination of the optimized ectoine secretory type halomonas as the stress-resistant factor and the SND denitrifying bacteria to construct a stress-resistant assisted SND denitrifying mode, thereby providing a high-salt nitrogen-containing wastewater denitrifying technology.
② anti-adversity helper factor tetrahydropyrimidine is provided in situ by the strain Halomonas venusta DSM4743, without adding tetrahydropyrimidine, and has the advantages of simple operation, low cost and remarkable effect.
③ the method can be widely used in the field of purification treatment of high-salt nitrogen-containing wastewater, such as wastewater in industries of magnesium oxide desulfurization waste liquid of marine diesel engines, leather processing, marine product cultivation, synthetic ammonia, fertilizer production, refuse landfill, nickel battery production, etc., and has wide application prospect.
Drawings
FIG. 1 shows SND denitrification of desulfurized waste liquid based on stress-resistant assistance by tetrahydropyrimidine-secreting strains;
FIG. 2 shows the influence of the addition ratio of the combined strains on the SND denitrification efficiency of the combined strains;
FIG. 3 shows SND denitrification of desulfurization waste liquid of different combinations of bacterial strains.
Detailed Description
The experimental materials used in the examples of the present invention were obtained commercially or prepared by a conventional method, unless otherwise specified, wherein:
the strain is as follows: halomonas venusta DSM4743 is available from DSMZ, Germany (German Collection of Microorganisms and Cell Cultures).
The strain is as follows: acinetobacter calcoaceticus CCTCC AB 205304(Acinetobacter calcoaceticus CCTCC 205304) was purchased from the China center for type culture Collection.
The strain is as follows: pseudomonas marinus MCCC1A02281 (Pseudomonas marincola MCCC1A 02281) was purchased from China center for culture Collection of marine microorganisms.
Medium A (g/L): sterilizing peptone 10, yeast powder 5, NaCl 60, pH 7.2, 121 deg.C, and 20 min.
Medium B (g/L): (NH)4)2 SO 43, monosodium glutamate 10, MgSO4·7H2O 0.05，K2HPO49，KH2PO43，MnSO4·4H2O 0.01，FeSO40.01, NaCl 30, pH 8.0, 121 ℃,20 min sterilization.
Medium C (g/L): (NH)4)2SO44, monosodium glutamate 8, trisodium citrate 30, K2HPO49，KH2PO43，MgSO4·7H2O 0.4，MnSO4·H20.01 of O, 0.5 of yeast powder, 30 of NaCl and trace elements (Na)2EDTA 63.7，ZnSO42.2，CaCl25.5，MnCl2·4H2O 5.06，FeSO4·7H2O 5；Na2MoO4·2H2O 1.1，CuSO4·5H2O 1.57，CoCl2·6H2O1.61) 2mL, pH 8.0, 121 ℃,20 min sterilization.
Tetrahydropyrimidine standard: purchased from Sigma-Aldrich, CAS: 96702-03-3.
Example 1 Synthesis and secretion experiments of Halomonas venusta DSM4743 tetrahydropyrimidine
The strain is as follows: halomonas venusta DSM4743
Strain activation: culture medium A, 30 ℃, 120r/min, 24 h.
And (3) tetrahydropyrimidine induction culture: the activated bacterial liquid is inoculated into a culture medium B (30mL/300mL) according to the inoculation amount of 1 percent, and the temperature is 30 ℃, 120r/min and 48 h.
And (3) determining the concentration of tetrahydropyrimidine:
intracellular tetrahydropyrimidine: 1mL of the fermentation broth was centrifuged (4 ℃, 14000 Xg, 15min), 1mL of 80% ethanol (v/v) was added to the precipitate for resuspension, and the mixture was allowed to stand at room temperature overnight. The suspension was centrifuged again and the supernatant was taken for HPLC determination.
Extracellular tetrahydropyrimidine: 1mL of the fermentation broth was centrifuged (4 ℃, 14000 Xg, 15min), and the supernatant was diluted 10-fold with deionized water and used for HPLC assay.
The total ectoine concentration is the sum of the intracellular and extracellular concentrations. The ecto-tetrahydropyrimidine secretion rate is the percentage of the ecto-tetrahydropyrimidine concentration in the total concentration.
High Performance Liquid Chromatography (HPLC) tetrahydropyrimidine concentration:
TSK-GEL reversed phase column (TOSOH corporation, Japan) is adopted, 50mmol/L and phosphate buffer solution with pH 6.0 is adopted as a mobile phase, the column temperature is 35 ℃, the flow rate is 1mL/min, and an ultraviolet detector detects the wavelength at 210 nm. The retention time of the tetrahydropyrimidine is obtained by detecting a tetrahydropyrimidine standard substance
The experimental results are as follows: the results of experiments on the synthesis and secretion of Halomonas venusta DSM4743 tetrahydropyrimidine were carried out as described above and showed that the intracellular tetrahydropyrimidine concentration was 179.5mg/L, the extracellular tetrahydropyrimidine concentration was 775.3mg/L, the total synthesized amount of tetrahydropyrimidine was 954.8mg/L, and the tetrahydropyrimidine secretion rate was 81.2%. The experimental result shows that the strain Halomonasvenusta DSM4743 is a tetrahydropyrimidine secreting strain and can efficiently synthesize and secrete tetrahydropyrimidine.
Example 2 Acinetobacter calcoaceticus CCTCC AB 205304 and Pseudomonas inhabitans MCCC1A02281 absorption of tetrahydropyrimidine experiments
To examine whether the SND denitrifying strains Acinetobacter calcoaceticus CCTCC AB 205304 and Pseudomonas inhabita MCCC1A02281 can absorb tetrahydropyrimidine, the following experiment was performed.
The strain is as follows: acinetobacter calcoaceticus CCTCC AB 205304, Pseudomonas judae MCCC1A 02281.
Strain activation: culture medium A (NaCl concentration adjusted to 5g/L), 30 ℃, 120r/min, 24 h.
Cell culture: the activated strains are respectively inoculated into a culture medium A (NaCl concentration is adjusted to be 5g/L) according to the inoculation amount of 1 percent, and cultured for 48 hours at 30 ℃ and 120 r/min.
Absorption of tetrahydropyrimidine: the cell culture medium was centrifuged at 14000 Xg at 4 ℃ for 15min, the supernatant was discarded, and the cells were washed 2 times with NaCl-KpiBuffer (100mM, pH 7.2, NaCl 5 g/L). Then, the cells were transferred to 30mL NaCl-Kpi Buffer (100mM, pH 7.2) containing 30g/L NaCl and 300mg/L tetrahydropyrimidine at 30 ℃ and 60rpm for 30min, so that the cells absorbed tetrahydropyrimidine under the stress of 30g/L NaCl.
The amount of tetrahydropyrimidine taken up by the cells was determined by the method for measuring intracellular tetrahydropyrimidine concentration in example 1.
The experimental results are as follows: the results of the ectoine uptake experiments performed as described above show that both Acinetobacter calcoaceticus CCTCCAB 205304 and Pseudomonas marinus MCCC1A02281 are capable of uptake ectoine into cells from the outside of the cells under NaCl stress. The intracellular tetrahydropyrimidine concentration of the acinetobacter calcoaceticus CCTCC AB 205304 is 26.3mg/g, and the concentration of the pseudomonas judae MCCC1A02281 is 29 mg/g. In contrast, in the control sample which had not undergone the absorption test, no tetrahydropyrimidine was detected in the cells.
Example 3 stress resistance of tetrahydropyrimidine-secreting strains on growth of SND denitrifying strains at different salt concentrations
The tetrahydropyrimidine-secreting strain (Halomonas venusta DSM 4743) was inoculated in a mixture with the SND denitrifying strain (Acinetobacter calcoaceticus CCTCC AB 205304 and Pseudomonas inhabita MCCC1A 02281) (medium C containing 0, 15, 30, 45, 60, 75, 90g/L NaCl, respectively). In the combined culture system, a strain Halomonas venusta DSM4743 synthesizes and secretes tetrahydropyrimidine under the induction of NaCl, and acinetobacter calcoaceticus CCTCC AB 205304 and pseudomonas juveniae MCCC1A02281 absorb the tetrahydropyrimidine secreted into the culture to obtain the stress resistance and salt-tolerant growth. The control experiment of salt-tolerant growth is that the SND denitrifying strain or the tetrahydropyrimidine secreting strain is cultured independently.
The strain is as follows: halomonas venusta DSM4743, Acinetobacter calcoaceticus CCTCC AB 205304, Pseudomonas inhabita MCCC1A 02281.
Strain activation: experimental strain activation methods Halomonas venusta DSM4743 and Acinetobacter calcoaceticus CCTCC AB 205304 and Pseudomonas judae MCCC1A02281 and example 2.
Culturing under high salt: acinetobacter calcoaceticus CCTCC AB 205304, Pseudomonas judai MCCC1A02281 and Halomonas venusta DSM4743 were separately cultured, and Halomonas venusta DSM4743 was mixed with Acinetobacter calcoaceticus CCTCC AB 205304 and Pseudomonas judai MCCC1A02281, respectively, and cultured. Culture medium C was used for all the cultures, and the NaCl concentrations of culture medium C were adjusted to 0, 15, 30, 45, 60, 75, and 90g/L, respectively. The inoculation amount is 1 percent, the temperature is 30 ℃, the speed is 120r/min, and the culture is carried out for 48 hours.
Measuring cell growth amount: the culture was centrifuged at 14000 Xg for 15min at 4 ℃ and the cells were washed with NaCl-Kpi Buffer (100mM, pH 7.2, NaCl concentration the same as the medium concentration), centrifuged, precipitated, dried at 105 ℃ to constant weight and weighed. The cell growth was defined as dry cell weight per liter of fermentation broth (g/L).
The experimental results are as follows: the results of the resistance assistance experiment of the tetrahydropyrimidine secreting strain on the growth of the SND denitrifying strain under high salt are shown in Table 1.
TABLE 1 stress-resistance assistance of tetrahydropyrimidine-secreting strains to SND denitrifying strains grown at high salt
Note: "-" is not detected.
The experimental result shows that the single acinetobacter calcoaceticus CCTCC AB 205304 basically does not grow in the culture medium with NaCl concentration more than or equal to 30 g/L; the growth amount of the single Pseudomonas putida MCCC1A02281 in the culture medium with NaCl concentration more than or equal to 30g/L is obviously reduced along with the increase of the NaCl concentration. The acinetobacter calcoaceticus CCTCC AB 205304 has no salt-tolerant growth capability, and the pseudomonas putida MCCC1A02281 has low salt-tolerant growth capability. Halomonas venusta DSM4743 showed growth in medium C with different NaCl concentrations, with an optimum growth NaCl concentration of 60 g/L. The Halomonas venusta DSM4743 is respectively mixed and cultured with Acinetobacter calcoaceticus CCTCC AB 205304 and Pseudomonas judae MCCC1A02281, and the cell growth amount in the culture medium is obviously improved. The difference between the growth amounts of the mixed culture and the single culture is the anti-adversity assisted growth amount of the tetrahydropyrimidine secreting type halophila to the denitrified strain. In the combined growth mode, the anti-adversity growth assisting amount of the ectoine secreted strain Halomonas venusta DSM4743 to the Acinetobacter calcoaceticus CCTCC AB 205304 is 1.3 to 3.4g/L and the anti-adversity growth assisting amount to the Pseudomonas inhabitalis MCCC1A02281 is 0.8 to 1.9g/L in the range of NaCl concentration of 15 to 60 g/L. Table 1 shows the concentration of tetrahydropyrimidine secreted into the medium by the strain Halomonas venusta DSM4743 in both combined culture systems.
Example 4 stress-resistant helper experiments on the denitrification of SND denitrifying strains at different salt concentrations by tetrahydropyrimidine-secreting strains
The SND denitrification experiment was performed using tetrahydropyrimidine secreting strains (Halomonas venusta DSM 4743) in combination with SND denitrification strains (acinetobacter calcoaceticus CCTCC AB 205304 and pseudomonas judae mcc 1a 02281). In the combined culture system, a strain Halomonas venusta DSM4743 synthesizes and secretes tetrahydropyrimidine under the induction of NaCl, and acinetobacter calcoaceticus CCTCC AB 205304 and pseudomonas juveniae MCCC1A02281 absorb the tetrahydropyrimidine secreted into the culture to obtain the stress resistance and salt-tolerant denitrification. The control experiment of the salt-tolerant denitrification is that the SND denitrification bacterial strain or the ectoine secretion type bacterial strain is subjected to single denitrification.
The strain is as follows: halomonas venusta DSM4743, Acinetobacter calcoaceticus CCTCC AB 205304, Pseudomonas inhabita MCCC1A 02281.
Strain activation: the procedure for activating the experimental strains was the same as in example 3.
Culture medium C of denitrification substrate (NH)4)2SO4The concentration was adjusted to 10g/L, and the NaCl concentration was adjusted to 0, 15, 30, 45, 60, 75, and 90g/L, respectively.
SND denitrification: acinetobacter calcoaceticus CCTCC AB 205304, Pseudomonas judai MCCC1A02281, and Halomonas venusta DSM4743 were denitrified separately and Halomonas venusta DSM4743 was denitrified in combination with Acinetobacter calcoaceticus CCTCC AB 205304 and Pseudomonas judai MCCC1A02281, respectively. The inoculation amount is 1 percent, the temperature is 30 ℃, the speed is 120r/min, and the culture is carried out for 48 hours.
Ammoniacal Nitrogen (NH)4 +-N) concentration determination: the method adopts a nano reagent method for determination. The ammonia nitrogen existing in the form of free ammonia or ammonium ions and the like reacts with the nano reagent to generate a light red brown complex. After the measurement sample is diluted properly, a Naeser reagent is added, and after standing for 10min at room temperature, the absorbance is measured at 420nm using a 1cm cuvette. Standard Curve (APHA: Stan) was prepared using ammonium chloride standard solutiondard methods for the examination of water and wastewater,20thed.,American Public Health Association,Washington,D.C.(1999).】。
Nitrite Nitrogen (NO)2 --N) concentration determination: the measurement is carried out by a diazotization coupling reaction method. Under the acidic condition, carrying out diazotization reaction on nitrite and sulfanilamide, and reacting a reaction product with 1-naphthylethylenediamine dihydrochloride to generate the deep red azo dye. After the test sample is properly diluted, the aminobenzenesulfonamide solution is firstly added, the mixture is stood for 5min at room temperature, then the 1-naphthylethylenediamine dihydrochloride solution is added, the mixture is stood for 15min at room temperature, and then a 1cm cuvette is used for measuring the light absorption value at 543 nm. Standard methods for the expert of water and water,20th ed., American Public Health Association, Washington, D.C. (1999) were used to generate a Standard curve using sodium nitrite Standard solution.
Nitrate Nitrogen (NO)3 --N) concentration determination: and (3) measuring by adopting a zinc-cadmium reduction method. The nitrate was quantitatively reduced to nitrite using cadmium-plated zinc plates, and the total nitrite and the original nitrite concentration were measured by the diazo-azo method described above to calculate the nitrate concentration [ Sun, H.F., Wang, H.W., and Yuan, C.Y. ] Optimization of zinc-cadmium reduction method for determination of nitrate in seawater, adv.Mater.Res.2013864-867, 1004-1007 ].
And (3) measuring the nitrogen content of the cells: total cellular nitrogen was determined using the Kjeldahl method [ AOAC: Official Methods of analysis,15th ed., Association of Official Analytical Chemist, Arlington, V.A.1990 ]. And (3) taking different CDW cells to measure the total nitrogen of the cells, and fitting a relational expression of the cell growth amount and the total nitrogen of the cells. The total cell nitrogen was calculated by measuring CDW during the experiment.
And (3) N balance calculation: the nitrogen balance equation of the denitrification system is as follows: TN (twisted nematic)0(initial total inorganic Nitrogen) ═ CN (cellular Total Nitrogen) + TNt(Final Total inorganic Nitrogen) + GN (overflowed gaseous Nitrogen) [ Jin, R.F., Liu, T.Q., Liu, G.F., Zhou, J.T., Huang, J.Y., and Wang, A.J.: Simultaneous serotropic and aerogenic determination by the mineral origin bacterium Pseudomonas sp.ADN-42, appl.biochem.Biotechnol.Biotechnol2015,175, 2000-2011. Wherein total inorganic nitrogen is defined as NH4 +-N、NO- 2-N、NO- 3-the sum of N.
The denitrification rate is as follows: denitrogenation rate (TN)0－CN－TNt)/(TN0-CN). times.100%. Wherein TN0For total inorganic nitrogen at the beginning of denitrification, CN is total cell nitrogen, TNtThe total inorganic nitrogen at the end of denitrification (the nitrogen consumption for cell growth is not included in the calculation of the denitrification rate).
The experimental results are as follows: the results of the resistance assistance experiment of the tetrahydropyrimidine secreting strain to the denitrification of the SND denitrifying strain under different salt concentrations are shown in the table 2.
Table 2 resistance assistance of denitrification at different salt concentrations (denitrification rate,%)
Note: "-" is not detected.
Experimental results show that the denitrification rate of the independent acinetobacter calcoaceticus CCTCC AB 205304 and the independent pseudomonas judae MCCC1A02281 in the denitrification matrix with NaCl concentration more than or equal to 15g/L is obviously reduced. Halomonas venusta DSM4743 exhibits a certain denitrification capacity in denitrification substrates of different NaCl concentrations. The strain Halomonas venusta DSM4743 is respectively denitrified by combining with Acinetobacter calcoaceticus CCTCC AB 205304 and Pseudomonas judae MCCC1A02281, and the difference of the denitrification rate of the combined denitrification and the denitrification rate of the single denitrification is the salt-tolerant denitrification after the deamination strain absorbs tetrahydropyrimidine. In the combined denitrification mode, the NaCl concentration is in the range of 15-60g/L, the anti-adversity assisted denitrification rate of the tetrahydropyrimidine secreting strain Halomonas venusta DSM4743 to the Acinetobacter calcoaceticus CCTCC AB 205304 is 14.1-34.9%, and the anti-adversity assisted denitrification rate to the Pseudomonas inhabitans MCCC1A02281 is 7-25.5%.
Example 5 Effect of inoculation method in Combined Denitrification on growth and Denitrification of Denitrification Strain
The strain secreting tetrahydropyrimidine (Halomonas venusta DSM 4743) was cultured in combination with SND denitrifying strain (Acinetobacter calcoaceticus CCTCC AB 205304 and Pseudomonas inhabita MCCC1A 02281) to perform SND denitrifying experiments. The effect of different inoculation patterns of the two strains on growth and denitrification was examined. Inoculation was done in two ways: firstly, simultaneous inoculation is carried out, namely two combined strains are simultaneously and respectively inoculated into a denitrification culture medium C (the inoculation amount is 1 percent) when SND denitrification begins; and secondly, staggered inoculation, namely only inoculating Halomonas venusta DSM4743 when SND denitrification begins, culturing for 6-18h, and then inoculating acinetobacter calcoaceticus CCTCC AB 205304 (or Pseudomonas judae MCCC1A 02281).
The strain is as follows: halomonas venusta DSM4743, Acinetobacter calcoaceticus CCTCC AB 205304, Pseudomonas inhabita MCCC1A 02281.
Strain activation: the same as in example 3.
The denitrification substrate is culture medium C, (NH)4)2SO410g/L, NaCl concentration 30, 60, 90 g/L. The SND denitrification method is the same as that of example 4. The ammonia nitrogen concentration was measured in the same manner as in example 4. The results are shown in Table 3.
TABLE 3 Effect of inoculation patterns on Strain growth and SND denitrification Rate
The anti-adversity assistance effect of the tetrahydropyrimidine secretory strain on the growth and denitrification of the SND denitrification strain under high salt is related to the inoculation mode, and staggered inoculation is better than simultaneous inoculation. Because of staggered inoculation, Halomonas venusta DSM4743 synthesizes and secretes tetrahydropyrimidine for the first 6-18h of culture, and acinetobacter calcoaceticus CCTCC AB 205304 (or pseudomonas judae MCCC1A 02281) is inoculated under the condition that the culture medium contains tetrahydropyrimidine, the two bacteria can rapidly grow because of rapidly absorbing tetrahydropyrimidine, and the rapid growth is favorable for improving the denitrification rate. The staggered inoculation is carried out within the range of 6-18h, and the cell growth amount is improved by 4.1-22.8% and the denitrification rate is improved by 8.5% -15.7% by 48 h. The preferred staggered inoculation time is 12h, the maximum growth amount is 12.7g/L, and the maximum denitrification rate is 92.5% (48 h).
Example 6 desulfurization waste stream SND combined denitrification experiment based on tetrahydropyrimidine secreting type strain stress resistance assistance
Ship firewoodThe magnesium oxide wet fuel oil desulfurization waste liquid of the oil engine is high-salt nitrogen-containing waste water (NH)4 +-N 460mg/L，NO2 --N 300mg/L，NO3 --N 600mg/L，Cl-34g/L)。
Mixing the tetrahydropyrimidine secreting strain Halomonas venusta DSM4743 with Acinetobacter calcoaceticus CCTCC AB 205304 and Pseudomonas inhabita MCCC1A02281 respectively according to equal proportion of the weight of the strain, and performing the desulfurization waste liquid denitrification experiment based on the anti-adversity assistance of the tetrahydropyrimidine secreting strain.
The strain is as follows: halomonas venusta DSM4743, Acinetobacter calcoaceticus CCTCC AB 205304, Pseudomonas inhabita MCCC1A 02281.
Strain activation and culture: the strain activation method was the same as in example 3. Culture medium C, 30 ℃, 120rpm, 24 h. When Halomonas venusta DSM4743 was cultured, the NaCl concentration was adjusted to 30 g/L.
SND denitrification of the desulfurization waste liquid: the activation and culture methods of the three strains were the same as in example 4. After the strain is cultured for 24 hours, centrifugally collecting cells, mixing the cells according to equal proportion of the weight of the strain, transferring the mixed cells into the desulfurization waste liquid, and adding 10g/L trisodium citrate (denitrification energy source) 6g/L K into the desulfurization waste liquid2HPO4，2g/L KH2PO4(cell growth inorganic phosphorus is provided). SND denitrification was performed at 30 ℃ and 120rpm under oxygen supply and organic carbon source. The ammonia nitrogen determination method is the same as in example 4.
The experimental results are as follows: the tetrahydropyrimidine secreting strain Halomonas venusta DSM4743 and SND are respectively combined with denitrifying strains of acinetobacter calcoaceticus CCTCC AB 205304 and Pseudomonas inhabita MCCC1A02281 to be used for purifying the magnesium oxide wet fuel oil desulfurization waste liquid SND of the marine diesel engine, and the result is shown in figure 1. As can be seen in FIG. 1, the denitrification rates for 120h denitrogenation, Acinetobacter calcoaceticus CCTCC AB 205304 and Pseudomonas marinus MCCC1A02281 were 14.1% and 43.2%, respectively, and the denitrification rate for Halomonas venusta DSM4743 alone was 57.1%. And the tetrahydropyrimidine secretory strain Halomonasvenusta DSM4743 is respectively denitrified by combining with the SND denitrification strain Acinetobacter calcoaceticus CCTCC AB 205304 and the Pseudomonas inhabita MCCC1A02281, so that the denitrification rate is obviously improved and reaches 89.3 percent and 92.3 percent respectively. With a single strainCompared with denitrification, the combined denitrification and denitrification rate is respectively improved by 75.2 percent and 49.1 percent. The denitrification efficiency is respectively improved from 1.6 and 4.9 to 10.1 and 10.5 mg/L.h. And in the whole SND denitrification process, NO2 -The concentration of N is less than or equal to 4.1mg/L, and the pH change range in the denitrification process is small (pH 6.5-8.5).
Example 7 Effect of the addition ratio of Strain on the SND denitrification Rate of combination bacteria
The magnesium oxide wet fuel desulfurization waste liquid of the marine diesel engine is high-salt nitrogen-containing waste water (NH)4 +-N 460mg/L，NO2 --N 300mg/L，NO3 --N 600mg/L，Cl-34g/L)。
The ectoine secreting strain Halomonas venusta DSM4743 and the SND denitrifying strain Pseudomonas marinus MCCC1A02281 are combined to carry out desulfurization waste liquid denitrification experiments based on the anti-stress assistance of the ectoine secreting strain.
The strain is as follows: halomonas venusta DSM4743, Acinetobacter calcoaceticus CCTCC AB 205304, Pseudomonas inhabita MCCC1A 02281.
SND denitrification of the desulfurization waste liquid: the activation and cultivation of the strain were carried out as in example 6. After the strains are cultured for 24 hours, cells are collected by centrifugation, the two strains are transferred into the desulfurization waste liquid (30mL/300mL) according to different addition amounts, and 10g/L trisodium citrate (denitrification energy source) 6g/L K g is added into the desulfurization waste liquid2HPO4，2g/L KH2PO4(cell growth inorganic phosphorus is provided). SND denitrification was performed at 30 ℃ and 120rpm under oxygen supply and organic carbon source. The ammonia nitrogen concentration was measured in the same manner as in example 4.
The addition ratio of the combined strains is as follows: cell mass (g/L) of the strain Halomonas venusta DSM 4743: the cell amount (g/L) of the pseudomonas putida MCCC1A02281 is 1:0.5(4g/L and 2g/L) respectively; 1:1(3g/L and 3 g/L); 1:2(2g/L and 4 g/L).
The experimental results are as follows: the 60h SND denitrification rate is 69.5 percent, 83.7 percent and 75.7 percent respectively according to three different addition ratios (1:0.5,1:1,1:2), wherein the highest denitrification rate is achieved when the addition ratio is 1: 1.
In the experiment of the influence of the addition ratio of the strains on the SND denitrification rate of the combined bacteria, the Pseudomonas putida MCCC1A02281 is replaced by Acinetobacter calcoaceticus CCTCC AB 205304, the 60hSND denitrification rate is respectively 63.9%, 81.2% and 72.3% in three different addition ratios (1:0.5,1:1,1:2), and the denitrification rate is the highest when the addition ratio is 1: 1.
Example 8 experiment of influence of different combinations of strains on SND denitrification of desulfurized waste liquid
The denitrification effect of a single denitrification strain (one strain), a combination of tetrahydropyrimidine-secreting halomonas with one denitrification strain (a combination of two strains), and a combination of tetrahydropyrimidine-secreting halomonas with two denitrification strains (a combination of three strains) was examined.
The magnesium oxide wet fuel desulfurization waste liquid of the marine diesel engine is high-salt nitrogen-containing waste water (NH)4 +-N 460mg/L，NO2 --N 300mg/L，NO3 --N 600mg/L，Cl-34g/L)。
The strain is as follows: (1) strain ectoine-secreting strain Halomonas venusta DSM4743, (2) Acinetobacter calcoaceticus CCTCC AB 205304, and (3) Pseudomonas inhabita MCCC1A 02281. The activation and cultivation methods were the same as in example 6.
Combining strains: combining the strains (1) and (2) by 3g/L respectively; combining the strains (1) and (3) by 3g/L respectively; strains (1), (2) and (3) were each combined at 2 g/L.
SND denitrification of the desulfurization waste liquid: the cells in different combination modes are respectively transferred into the desulfurization waste liquid, and 10g/L trisodium citrate (denitrification energy source) 6g/L K is added into the desulfurization waste liquid2HPO4，2g/L KH2PO4(cell growth inorganic phosphorus is provided). SND denitrification was carried out at 30 ℃ and 120rpm under oxygen supply and organic carbon source (60 h). The ammonia nitrogen determination method is the same as in example 4.
The experimental results are as follows: the denitrification effect of different combinations of strains is shown in FIG. 3. As can be seen from FIG. 3, the denitrification rates of the single strain, the combination of two strains, and the combination of three strains were in the range of 12.4% to 95.1%. The highest denitrification rate (95.1%) of the combination of the three strains indicates that the combination of the tetrahydropyrimidine secreting type halomonas and a plurality of traditional denitrification strains is more beneficial to denitrification.
Claims (7)
1. The combined flora for stress-resistant assisted denitrification of high-salt nitrogen-containing wastewater is characterized in that: the strain comprises a tetrahydropyrimidine secreting strain and an SND denitrifying strain, wherein the tetrahydropyrimidine secreting strain is Halomonas venusta DSM4743 of Halomonas, and the SND denitrifying strain is acinetobacter calcoaceticus CCTCC AB 205304 and/or pseudomonas jubilans MCCC1A 02281.
2. The combined population of claim 1, wherein: when one strain is selected from the tetrahydropyrimidine secreting strain and the SND denitrifying strain, the inoculation ratio is 1: 0.5-2 by weight.
3. The combined population of claim 1, wherein: when one strain is selected from the tetrahydropyrimidine secreting strains and two strains are selected from the SND denitrifying strains, the inoculation ratio is equal to the weight ratio.
4. The application of the combined flora of claim 1 in the technical fields of treating high-salt nitrogen-containing wastewater and purifying mariculture water bodies.
5. Use according to claim 4, characterized in that: when high-salt nitrogen-containing wastewater is treated and a mariculture water body is purified, a staggered inoculation mode of inoculating a tetrahydropyrimidine secretory strain first and then inoculating an SND denitrification strain is adopted.
6. Use according to claim 5, characterized in that: the interval time of the staggered inoculation is 6-18 h.
7. Use according to claim 5, characterized in that: the high-salt nitrogen-containing wastewater is wastewater with NaCl concentration more than or equal to 30g/L and nitrogen element concentration less than or equal to 3000 mg/L.
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| CN109607755B (en) * | 2019-02-19 | 2022-04-29 | 大连海事大学 | Method for efficiently generating electricity and denitrifying by microbial fuel cell under high salt condition |
| CN110156174B (en) * | 2019-05-30 | 2022-01-25 | 杭州秀川科技有限公司 | Mixed strain fermentation biological pretreatment method for high-concentration high-salt pharmaceutical wastewater |
| CN110982732B (en) * | 2019-11-13 | 2022-12-30 | 重庆理工大学 | Salt-tolerant and high-ammonia-nitrogen-resistant heterotrophic nitrification-aerobic denitrification composite microbial agent and preparation and application thereof |
| CN112175863B (en) * | 2020-09-15 | 2022-05-13 | 重庆理工大学 | Preparation method and application of complex microbial inoculant for removing nitrogen-containing pollutants in high-salinity wastewater |
| CN113913335B (en) * | 2021-10-20 | 2023-07-07 | 大连海洋大学 | Salt-tolerant nitrifying composite microbial agent and application thereof |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101314785A (en) * | 2008-07-22 | 2008-12-03 | 大连海事大学 | Method for fermentation preparation of Ectoine |
| CN101407362A (en) * | 2008-11-04 | 2009-04-15 | 山东大学 | Method for treating papermaking waste water by constructing artificial flora |
| CN101629153A (en) * | 2009-06-11 | 2010-01-20 | 浙江大学 | Method for processing preserved szechuan pickle waste water by compound bacterial flora |
| CN103589669A (en) * | 2013-10-27 | 2014-02-19 | 沈阳建筑大学 | Salt-resistant denitriding composite inoculant, and preparation method and use method thereof |
| CN104911130A (en) * | 2015-06-10 | 2015-09-16 | 国家海洋局第三海洋研究所 | Halomonas sp. with denitrogenation capability and application thereof |
-
2016
- 2016-12-19 CN CN201611180029.8A patent/CN106754504B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101314785A (en) * | 2008-07-22 | 2008-12-03 | 大连海事大学 | Method for fermentation preparation of Ectoine |
| CN101407362A (en) * | 2008-11-04 | 2009-04-15 | 山东大学 | Method for treating papermaking waste water by constructing artificial flora |
| CN101629153A (en) * | 2009-06-11 | 2010-01-20 | 浙江大学 | Method for processing preserved szechuan pickle waste water by compound bacterial flora |
| CN103589669A (en) * | 2013-10-27 | 2014-02-19 | 沈阳建筑大学 | Salt-resistant denitriding composite inoculant, and preparation method and use method thereof |
| CN104911130A (en) * | 2015-06-10 | 2015-09-16 | 国家海洋局第三海洋研究所 | Halomonas sp. with denitrogenation capability and application thereof |
Non-Patent Citations (7)
| Title |
|---|
| 2-Methoxy-3-(1-methylpropyl)pyrazine, pea odour, from the marine bacterium Halomonas venusta;Michael Bungert et al.;《FLAVOUR AND FRAGRANCE JOURNAL》;20011231;第16卷;第329-333页 * |
| Biofilm formation on polystyrene in detached vs. planktonic cells of polyhydroxyalkanoate-accumulating Halomonas venusta;Mercedes Berlanga et al.;《International Microbiology》;20141231;第17卷;第205-212页 * |
| Halomonas venusta DSM4743渗透压冲击下四氢嘧啶合成与释放;郑昕等;《微生物学通报》;20100720;第37卷(第7期);第1090-1096页 * |
| 一株中度嗜盐菌SL21合成Ectoine的研究;周月慧等;《河南工业大学学报（自然科学版）》;20060430;第27卷(第2期);第81-85页 * |
| 中度嗜盐菌分离鉴定及在环境修复中的应用;刘莹莹;《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》;20130915(第9期);B027-53 * |
| 中度嗜盐菌四氢嘧啶合成基因的克隆与功能分析;张薇等;《生物工程学报》;20080325;第24卷(第3期);第395-400页 * |
| 自生物絮团养殖池分离具有亚硝酸盐去除功能的细菌及其鉴定和特性;王志杰等;《渔业科学进展》;20150430;第36卷(第2期);第100-105页 * |
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