CN106751925A - A kind of edibility collagen activity composite membrane and preparation method thereof - Google Patents
A kind of edibility collagen activity composite membrane and preparation method thereof Download PDFInfo
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- CN106751925A CN106751925A CN201710024265.9A CN201710024265A CN106751925A CN 106751925 A CN106751925 A CN 106751925A CN 201710024265 A CN201710024265 A CN 201710024265A CN 106751925 A CN106751925 A CN 106751925A
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L89/00—Compositions of proteins; Compositions of derivatives thereof
- C08L89/04—Products derived from waste materials, e.g. horn, hoof or hair
- C08L89/06—Products derived from waste materials, e.g. horn, hoof or hair derived from leather or skin, e.g. gelatin
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J5/00—Manufacture of articles or shaped materials containing macromolecular substances
- C08J5/18—Manufacture of films or sheets
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2389/00—Characterised by the use of proteins; Derivatives thereof
- C08J2389/04—Products derived from waste materials, e.g. horn, hoof or hair
- C08J2389/06—Products derived from waste materials, e.g. horn, hoof or hair derived from leather or skin
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2405/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2401/00 or C08J2403/00
- C08J2405/16—Cyclodextrin; Derivatives thereof
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L2201/00—Properties
- C08L2201/06—Biodegradable
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L2201/00—Properties
- C08L2201/08—Stabilised against heat, light or radiation or oxydation
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L2203/00—Applications
- C08L2203/16—Applications used for films
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Manufacturing & Machinery (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
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Abstract
The invention provides a kind of edibility collagen activity composite membrane and preparation method thereof, collagen, betadex, curcumin, Tween 80 and glycerine are contained in its membrane component.The distilled water that curcumin and betadex mixing are dissolved in tween 80 is first made curcumin emulsifier solution, then collagen is dissolved in curcumin emulsifier solution and is made collagen solution, glycerine is added as plasticizer, stir, degassing is stood, is pipetted and is spread in drying and forming-film on lucite frame.Collagen activity composite membrane prepared by the present invention has good antioxygenic property, can be biodegradable, environment will not be polluted, external microbe can be prevented to be contacted with food, the oxidation that food can be suppressed is rotted, so as to reach the effect of food fresh keeping and Shelf-life.
Description
Technical field
The present invention relates to a kind of edibility collagen activity composite membrane and preparation method thereof, belong to bioactivity bag
Fill the applied technical field of film.
Background technology
As the development of modern food industry, packaging for foodstuff are constantly updated, a class can improve packaging material and environmental protection
Between contradiction novel foodstuff packing technique material --- edible packaging is shown one's talent.Edible packing material refers to when packaging
Functional realiey after, the material can be changed into a kind of animal or the edible raw material of people, be a kind of achievable packaging material function
The extra package material of transition.Edible packing material is a kind of packaging without discarded object, is a kind of bag of resource-type environment-friendly type
Package material.Edible packaging, you can with the packaging for eating, typically by starch, protein, polysaccharide, fat, complex class material group
Into, be widely used in such as preservative film, packing film, packaging for foodstuff, cake packaging, seasoning packaging.Edibility, security, can
The good characteristics such as biological degradability cause that edible film has wide DEVELOPMENT PROSPECT.But, few encapsulations, excellent of collecting
Good organoleptic attribute, microbial stability are in the edible packing membrane of one.In addition adaptive surface is narrower, and cost is high etc. makes it can't
Commercially it is widely used, it is impossible to replace synthesising packing material completely.This is primarily due to edible packing membrane and also deposits
In some shortcomings, for example:Moisture barrier property is poor, anti-oxidant and antibiotic property is bad etc..
The present invention is prepared for a kind of Isin glue collagen active packing film, the film with Isin glue collagen and curcumin as raw material
With good antioxygenic property, can be biodegradable, environment will not be polluted, external microbe can be prevented to be connect with food
Touch, suppress Food Oxidation and rot, so as to reach the effect of food fresh keeping and Shelf-life.
The content of the invention
Collagen activity composite membrane prepared by the present invention has good antioxygenic property, can be biodegradable, will not
Environment is polluted, external microbe can be prevented to be contacted with food, suppress Food Oxidation rot, so as to reach food fresh keeping and
The effect of Shelf-life.To achieve the above object, the present invention is adopted the following technical scheme that:
A kind of edibility collagen activity composite membrane, in membrane component containing collagen, curcumin, Tween 80 and
Glycerine.Collagen, curcumin, betadex, Tween-80 and each mass ratio of glycerine are 80:(0.1-1):2:20:
20。
Described described curcumin(curcumin)It is to extract the xanthein for obtaining in a kind of rhizome from turmeric.It is
Topmost curcumin(curcuminoid)Class material, accounts for the 70% of curcumin, about the 3%~6% of turmeric.Ginger
Flavine is orange-yellow crystalline powder, and taste is slightly bitter, water insoluble.
A kind of preparation method of edibility collagen antibacterial film as described above is comprised the following steps:
(1)The preparation of curcumin emulsifying agent:Weigh 0.5 ~ 5mg curcumins and 10mg betadexs are placed in the dry small burnings of 25ml
In cup, rock back and forth, mix its machinery, be subsequently adding 10ml water, and magnetic agitation 4h, cyclodextrin is fully dissolved, turmeric
Plain fully wetting, is subsequently adding 0.1g Tween-80s, continues to stir 24h, obtains final product curcumin emulsifying agent;
(2)The preparation of collagen solution:10 ml curcumin emulsifying agents, 45 DEG C of magnetic force are added in 0.4 g collagens
Stirring and dissolving is made collagen solution;
(3)The preparation of edibility collagen activity composite membrane:In step(2)In the collagen solution for obtaining, add
0.1g glycerine stirs as plasticizer, stand degassing, obtain composite membrane film casting solution, by casting solution spread into 120 mm ×
Film forming on 80 mm × 4mm lucite frames, film-forming temperature is 23 ± 0.5 DEG C, and film formation time is 48-24 h, and edibility fish is obtained
Collagen activity composite membrane.
The preparation technology of the collagen described in step (2) is as follows:Discarded object fish-skin is scaled, and shreds clean, use
20 times of raw material weights contain the H that volume fraction is 1%2O20.01 mol/L NaOH solutions 20 DEG C stirring immersion 24 h,
A not good liquor is changed per 8h, then neutrality is washed till with clear water;Immersion 4 is stirred at room temperature with the aqueous isopropanol of 20 times of raw material weights again
H, neutrality is washed till with clear water;10min is soaked with the acetum of 0.05mol/l again, neutrality is washed with water to;Then, at 10 times
12 h are extracted in 45 DEG C of hot water of raw material weight;Insoluble substance is removed using centrifugation, it is lyophilized to obtain final product collagen.
Described discarded object fish-skin is the processing fish-skin accessory substance of silver carp, grass carp, black carp, bighead, shark or eel.
Beneficial effects of the present invention:(1)Collagen activity composite membrane prepared by the present invention has good inoxidizability
Can, can be biodegradable, environment will not be polluted, external microbe can be prevented to be contacted with food, suppress Food Oxidation rotten
It is rotten, so as to reach the effect of food fresh keeping and Shelf-life.For example, Li Peng of University Of Hainan etc. is using gelatin film refrigeration sieve
Non- carp fillets and extend the freshness date of fillet.(2)Raw material in preparation method of the present invention(Collagen)Wide material sources,
Process is simple;Curcumin Nantural non-toxic, and with good antioxidation activity.
Brief description of the drawings
Fig. 1 is the edibility fish skin collagen compound oxidation resistance not of activity of embodiment 1.
Fig. 2 is the DPPH radical scavenging activities of the edibility fish skin collagen activity composite membrane of embodiment 2.
Specific embodiment
Embodiment 1
(1)The preparation of curcumin emulsifying agent:Weigh 0.5mg curcumins and 10mg betadexs are placed in the dry small beakers of 25ml
In, rock back and forth, its machinery is mixed, 10ml water, and magnetic agitation 4h are subsequently adding, cyclodextrin is fully dissolved, curcumin
Fully wetting, is subsequently adding 0.1g Tween-80s, continues to stir 24h, obtains final product curcumin emulsifying agent.
(2)The preparation of collagen solution:10 ml curcumin emulsifying agents, 45 DEG C are added in 0.4 g collagens
Magnetic agitation dissolving is made collagen solution;
(3)The preparation of edibility collagen activity composite membrane:In step(2)In the collagen solution for obtaining, add
0.1g glycerine stirs as plasticizer, stand degassing, obtain composite membrane film casting solution, by casting solution spread into 120 mm ×
Film forming on 80 mm × 4mm lucite frames, film-forming temperature is 23 ± 0.5 DEG C, and film formation time is 24 h, and edibility fish-skin is obtained
Collagen activity composite membrane.
Using two methods of ABTS and DPPH, the antioxidation effect of collagen activity composite membrane is investigated respectively
Really.Result is as shown in Figure 1 and Figure 2.The transparency at thickness, water solubility, elongation at break, 600 nm to the active membrane of preparation
And the index such as moisture-vapor transmission is determined, as a result as shown in table 1.
Embodiment 2
(1)The preparation of curcumin emulsifying agent:Weigh 1mg curcumins and 10mg betadexs are placed in the dry small beakers of 25ml
In, rock back and forth, its machinery is mixed, 10ml water, and magnetic agitation 4h are subsequently adding, cyclodextrin is fully dissolved, curcumin
Fully wetting, is subsequently adding 0.1g Tween 80s, continues to stir 24h, obtains final product curcumin emulsifying agent.
(2)The preparation of collagen solution:10 ml curcumin emulsifying agents, 45 DEG C are added in 0.4 g collagens
Magnetic agitation dissolving is made collagen solution;
(3)The preparation of edibility collagen activity composite membrane:In step(2)In the collagen solution for obtaining, add
0.1g glycerine stirs as plasticizer, stand degassing, obtain composite membrane film casting solution, by casting solution spread into 120 mm ×
Film forming on 80 mm × 4mm lucite frames, film-forming temperature is 23 ± 0.5 DEG C, and film formation time is 36 h, and edibility fish-skin is obtained
Collagen activity composite membrane.
Using two methods of ABTS and DPPH, the antioxidation effect of collagen activity composite membrane is investigated respectively
Really.Result is as shown in Figure 1 and Figure 2.The transparency at thickness, water solubility, elongation at break, 600 nm to the active membrane of preparation
And the index such as moisture-vapor transmission is determined, as a result as shown in table 1.
Embodiment 3
(1)The preparation of curcumin emulsifying agent:Weigh 2.5mg curcumins and 10mg betadexs are placed in the dry small beakers of 25ml
In, rock back and forth, its machinery is mixed, 10ml water, and magnetic agitation 4h are subsequently adding, cyclodextrin is fully dissolved, curcumin
Fully wetting, is subsequently adding 0.1g Tween 80s, continues to stir 24h, obtains final product curcumin emulsifying agent.
(2)The preparation of collagen solution:10 ml curcumin emulsifying agents, 45 DEG C are added in 0.4 g collagens
Magnetic agitation dissolving is made collagen solution;
(3)The preparation of edibility collagen activity composite membrane:In step(2)In the collagen solution for obtaining, add
0.1g glycerine stirs as plasticizer, stand degassing, obtain composite membrane film casting solution, by casting solution spread into 120 mm ×
Film forming on 80 mm × 4mm lucite frames, film-forming temperature is 23 ± 0.5 DEG C, and film formation time is 40h, and edibility fish glue from skin is obtained
Former protein active composite membrane.
Using two methods of ABTS and DPPH, the antioxidation effect of collagen activity composite membrane is investigated respectively
Really.Result is as shown in Figure 1 and Figure 2.The transparency at thickness, water solubility, elongation at break, 600 nm to the active membrane of preparation
And the index such as moisture-vapor transmission is determined, as a result as shown in table 1.
Embodiment 4
(1)The preparation of curcumin emulsifying agent:Weigh 5mg curcumins and 10mg betadexs are placed in the dry small beakers of 25ml
In, rock back and forth, its machinery is mixed, 10ml water, and magnetic agitation 4h are subsequently adding, cyclodextrin is fully dissolved, curcumin
Fully wetting, is subsequently adding 0.1g Tween 80s, continues to stir 24h, obtains final product curcumin emulsifying agent.
(2)The preparation of collagen solution:10 ml curcumin emulsifying agents, 45 DEG C are added in 0.4 g collagens
Magnetic agitation dissolving is made collagen solution;
(3)The preparation of edibility collagen activity composite membrane:In step(2)In the collagen solution for obtaining, add
0.1g glycerine stirs as plasticizer, stand degassing, obtain composite membrane film casting solution, by casting solution spread into 120 mm ×
Film forming on 80 mm × 4mm lucite frames, film-forming temperature is 23 ± 0.5 DEG C, and film formation time is 48h, and edibility fish glue from skin is obtained
Former protein active composite membrane.
Using two methods of ABTS and DPPH, the antioxidation effect of collagen activity composite membrane is investigated respectively
Really.Result is as shown in Figure 1 and Figure 2.The transparency at thickness, water solubility, elongation at break, 600 nm to the active membrane of preparation
And the index such as moisture-vapor transmission is determined, as a result as shown in table 1.
Table 1:The physical property of the antibacterial film prepared under different condition
The preferred embodiment of invention, all impartial changes done according to scope of the present invention patent and modification are the foregoing is only,
Covering scope of the invention should all be belonged to.
Claims (6)
1. a kind of edibility collagen activity composite membrane, it is characterised in that:Described active membrane component includes fish-skin
Collagen, curcumin, betadex, Tween-80 and glycerine.
2. edibility collagen according to claim 1 activity composite membrane, it is characterised in that:Described curcumin
It is that the xanthein for obtaining is extracted from turmeric rhizome.
3. edibility collagen according to claim 1 activity composite membrane, it is characterised in that:Fish skin collagen egg
In vain, curcumin, betadex, Tween-80 and each mass ratio of glycerine are 80:(0.1-1):2:20:20.
4. a kind of preparation method of edibility collagen as claimed in claim 1 activity composite membrane, it is characterised in that:
Described preparation method is comprised the following steps:
(1)The preparation of curcumin emulsifying agent:Weigh 0.5 ~ 5mg curcumins and 10mg betadexs are placed in the dry small burnings of 25ml
In cup, rock back and forth, mix its machinery, be subsequently adding 10ml water, and magnetic agitation 4h, cyclodextrin is fully dissolved, turmeric
Plain fully wetting, is subsequently adding 0.1g Tween-80s, continues to stir 24h, obtains final product curcumin emulsifying agent;
(2)The preparation of collagen solution:10 ml curcumin emulsifying agents, 45 DEG C of magnetic force are added in 0.4 g collagens
Stirring and dissolving is made collagen solution;
(3)The preparation of edibility collagen activity composite membrane:In step(2)In the collagen solution for obtaining, add
0.1g glycerine stirs as plasticizer, stand degassing, obtain composite membrane film casting solution, by casting solution spread into 120 mm ×
Film forming on 80 mm × 4mm lucite frames, film-forming temperature is 23 ± 0.5 DEG C, and film formation time is 24-48 h, and edibility fish is obtained
Collagen activity composite membrane.
5. the preparation method of edibility collagen according to claim 3 activity composite membrane, it is characterised in that:Step
Suddenly the preparation technology of the collagen described in (2) is as follows:Discarded object fish-skin is scaled, shred it is clean, with 20 times of raw material weights
That measures contains the H that volume fraction is 1%2O20.01 mol/L NaOH solutions in 20 DEG C of stirring 24 h of immersion, change once per 8h
Liquid, is then washed till neutrality with clear water;4 h of immersion are stirred at room temperature with the aqueous isopropanol of 20 times of raw material weights again, clear water is used
It is washed till neutrality;10min is soaked with the acetum of 0.05mol/l again, neutrality is washed with water to;Then, weighed in 10 times of raw materials
12 h are extracted in 45 DEG C of hot water;Insoluble substance is removed using centrifugation, it is lyophilized to obtain final product collagen.
6. the preparation method of edibility collagen according to claim 4 activity composite membrane, it is characterised in that:Institute
The discarded object fish-skin stated is the processing fish-skin accessory substance of silver carp, grass carp, black carp, bighead, shark or eel.
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Cited By (10)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107383895A (en) * | 2017-07-24 | 2017-11-24 | 青岛金典生化器材有限公司 | A kind of oxidation resistant collagen film and preparation method thereof |
CN109090206A (en) * | 2018-07-12 | 2018-12-28 | 福州大学 | A kind of collagen-base antioxidant coating and its preparation method and application |
CN109957130A (en) * | 2019-03-25 | 2019-07-02 | 尚好科技有限公司 | Edible Unidirectional water permeable polymeric membrane and preparation method thereof |
CN110437625A (en) * | 2019-06-25 | 2019-11-12 | 浙江省农业科学院 | A kind of edible film-coating and preparation method thereof |
CN111825989A (en) * | 2020-07-23 | 2020-10-27 | 广东石油化工学院 | Edible film and preparation method thereof |
CN112021391A (en) * | 2020-09-10 | 2020-12-04 | 上海海洋大学 | Natural biological preservative for aquatic products and preparation method thereof |
CN113024892A (en) * | 2021-03-24 | 2021-06-25 | 上海海洋大学 | Cellulose-based membrane with photodynamic antibacterial activity, preparation and application thereof |
CN113533320A (en) * | 2021-07-18 | 2021-10-22 | 福建农林大学 | Shrimp paste packaging material with photosensitive sterilization activity and freshness indication characteristics |
CN114698676A (en) * | 2022-03-23 | 2022-07-05 | 福州大学 | Preparation method and application of silver carp skin collagen-based antibacterial and antioxidant active coating |
CN115197453A (en) * | 2022-07-01 | 2022-10-18 | 陕西科技大学 | Intelligent slow-release active packaging film and preparation method and application thereof |
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Cited By (13)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107383895A (en) * | 2017-07-24 | 2017-11-24 | 青岛金典生化器材有限公司 | A kind of oxidation resistant collagen film and preparation method thereof |
CN109090206A (en) * | 2018-07-12 | 2018-12-28 | 福州大学 | A kind of collagen-base antioxidant coating and its preparation method and application |
CN109957130A (en) * | 2019-03-25 | 2019-07-02 | 尚好科技有限公司 | Edible Unidirectional water permeable polymeric membrane and preparation method thereof |
CN110437625B (en) * | 2019-06-25 | 2021-08-31 | 浙江省农业科学院 | Edible coating film and preparation method thereof |
CN110437625A (en) * | 2019-06-25 | 2019-11-12 | 浙江省农业科学院 | A kind of edible film-coating and preparation method thereof |
CN111825989B (en) * | 2020-07-23 | 2022-06-03 | 广东石油化工学院 | Edible film and preparation method thereof |
CN111825989A (en) * | 2020-07-23 | 2020-10-27 | 广东石油化工学院 | Edible film and preparation method thereof |
CN112021391A (en) * | 2020-09-10 | 2020-12-04 | 上海海洋大学 | Natural biological preservative for aquatic products and preparation method thereof |
CN113024892A (en) * | 2021-03-24 | 2021-06-25 | 上海海洋大学 | Cellulose-based membrane with photodynamic antibacterial activity, preparation and application thereof |
CN113533320A (en) * | 2021-07-18 | 2021-10-22 | 福建农林大学 | Shrimp paste packaging material with photosensitive sterilization activity and freshness indication characteristics |
CN114698676A (en) * | 2022-03-23 | 2022-07-05 | 福州大学 | Preparation method and application of silver carp skin collagen-based antibacterial and antioxidant active coating |
CN115197453A (en) * | 2022-07-01 | 2022-10-18 | 陕西科技大学 | Intelligent slow-release active packaging film and preparation method and application thereof |
CN115197453B (en) * | 2022-07-01 | 2023-05-16 | 陕西科技大学 | Intelligent slow-release type active packaging film and preparation method and application thereof |
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