CN106750579A - The preparation method of polypyrrole shitosan composite conductive thin film - Google Patents
The preparation method of polypyrrole shitosan composite conductive thin film Download PDFInfo
- Publication number
- CN106750579A CN106750579A CN201611169657.6A CN201611169657A CN106750579A CN 106750579 A CN106750579 A CN 106750579A CN 201611169657 A CN201611169657 A CN 201611169657A CN 106750579 A CN106750579 A CN 106750579A
- Authority
- CN
- China
- Prior art keywords
- shitosan
- pyrroles
- preparation
- thin film
- acetum
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L5/00—Compositions of polysaccharides or of their derivatives not provided for in groups C08L1/00 or C08L3/00
- C08L5/08—Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J5/00—Manufacture of articles or shaped materials containing macromolecular substances
- C08J5/18—Manufacture of films or sheets
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2305/00—Characterised by the use of polysaccharides or of their derivatives not provided for in groups C08J2301/00 or C08J2303/00
- C08J2305/08—Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08J—WORKING-UP; GENERAL PROCESSES OF COMPOUNDING; AFTER-TREATMENT NOT COVERED BY SUBCLASSES C08B, C08C, C08F, C08G or C08H
- C08J2479/00—Characterised by the use of macromolecular compounds obtained by reactions forming in the main chain of the macromolecule a linkage containing nitrogen with or without oxygen, or carbon only, not provided for in groups C08J2461/00 - C08J2477/00
- C08J2479/04—Polycondensates having nitrogen-containing heterocyclic rings in the main chain; Polyhydrazides; Polyamide acids or similar polyimide precursors
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08L—COMPOSITIONS OF MACROMOLECULAR COMPOUNDS
- C08L2203/00—Applications
- C08L2203/16—Applications used for films
Landscapes
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Manufacturing & Machinery (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Materials Engineering (AREA)
- Manufacture Of Macromolecular Shaped Articles (AREA)
Abstract
The present invention discloses a kind of preparation method of polypyrrole shitosan composite conductive thin film, comprises the following steps：Step one, the acetum for preparing shitosan；Step 2, in mass ratio to pyrroles is added in the acetum of shitosan, obtain pyrroles's chitosan solution；Step 3, in molar ratio to adding anhydrous ferric chloride in pyrroles's chitosan solution；Step 4, masking.The conductive composite film prepared using the inventive method has not only manufactured good microenvironment to fluorescence molecule makes it be difficult to influence fluorescence lifetime, and with preferable ductility and transparency.The basic standards such as small, the good biocompatibility of nontoxic, nonirritant, challeng are reached as the carrier of Imaging-PAM.Additionally, the present invention increases to the receptor protein in Imaging-PAM to the quantum efficiency of fluorescent material.Prepare simply, raw material is cheap, with good bioactivity and electric conductivity.
Description
Technical field
The invention belongs to biomedical sector, a kind of membrane material of fluorescence imaging is specifically related to.
Background technology
Composite is presently mainly finger conductive polymeric composite, is to pass through polymer with various conductive materials
Certain complex method is constituted.For a long time, macromolecular material is employed typically as insulating materials.However, for giving birth to
In the composite of thing medical research field, especially composite used in research in terms of cytology generally requires material
Material has certain electric conductivity in itself.It is well known that biological electricity is the essence of vital functions, it is also the base of human life activity
Plinth, any vital movement of human body is closely related without discord biological electricity.However, base unit of the cell as organism,
It is also bioelectric base unit, therefore, the composite related to biological electricity is studied on a cellular level, to a certain degree
On, it is necessary to composite has certain electric conductivity.Fluorescence imaging is that fluorescent material is excited by specific outside energy, causes it
Electronics can produce the fluorescence signal that can be detected to High-Energy Orbit transition, and during the recurrence ground state that finally releases energy.It is glimmering
Photoimaging technology has been obtained for being widely applied in biology and medical domain, is observation cellular morphology, structure and life
Powerful.Often FRET (FRET) is combined with fluorescence microscopy, regularly, quantified, positioningly seen
Survey protein kinase activity change in living cells.The locating information acquisition and quantitative determination of living cells amplifying nucleic acid and albumen in living cells
The microscopic imaging fluorescence analysis of matter is also the application of Imaging-PAM.
Shitosan is the product after chitin deacetylase base, belongs to semi-synthetic organic polymer, is chitin most basic, most
Important derivative.Shitosan has good biocompatibility, degradability, cheap, abundance and for renewable
Resource, is very important native biopolymer material in biomedical research.Conventional conducting materials are general in black, thickness
Thicker, translucency is poor, it is impossible to for cell fluorescence imaging experiment.
In the prior art, someone provides a kind of preparation method of chitosan-based composite, and acetic acid is first used in its method
Dissolving shitosan, then using NaOH as solidification liquid, dry chitosan material, then chitosan material is soaked into pyrroles
In, finally aoxidized in oxidant.Chitosan-based composite material film is prepared as described above, due to making using NaOH
It is solidification liquid, after shitosan drying in pelletized form, during follow-up immersion pyrroles, can not be fully mixed with pyrroles, the finished product for finally giving
In contain shitosan centrophyten, have a strata pyrroles, because shitosan is wrapped by, the biology of this film in its Surface coating
Compatibility is very poor, can not be used as the ideal carrier of cell growth.
The content of the invention
To solve above technical problem, it is multiple that the present invention provides a kind of polypyrrole-shitosan that can be used for cell fluorescence imaging
Close the preparation method of conductive film.
Technical scheme is as follows：
A kind of preparation method of polypyrrole-shitosan composite conductive thin film, it is critical only that and comprises the following steps：
Step one, the acetum for preparing shitosan；
Step 2, under the conditions of lucifuge, M in mass ratio (pyrroles)：M (shitosan)=1：The ratio of 4-6, to shitosan
Pyrroles is added in acetum, pyrroles-chitosan solution is obtained；
Step 3, in molar ratio n (pyrroles)：N (anhydrous ferric chloride)=1：The ratio of 3-5, it is molten to the pyrroles-shitosan
Anhydrous ferric chloride is added in liquid；
Step 4, masking.
Preferably, in above-mentioned steps one, the collocation method of the acetum of the shitosan is：1% is configured first
Aqueous acetic acid, then the acetum of 2% shitosan is configured with the aqueous acetic acid, finally by the acetum of the shitosan
In 50 DEG C of stirrings until dissolving.
In above-mentioned steps two, after the acetum of the shitosan is cooled to room temperature, then to the acetic acid of the shitosan
The pyrroles is added in solution, 1.5-3h is stirred.
In above-mentioned steps three, after adding anhydrous ferric chloride, 20-30h is stirred, oxidation obtains into film liquid.
In above-mentioned steps four, take described into film liquid, be added dropwise in the culture dish with sheet glass, the culture dish is placed on
Masking in sol evenning machine.
In above-mentioned steps two, the mass ratio of the pyrroles and shitosan is 1：5.
In above-mentioned steps three, the mol ratio of the pyrroles and anhydrous ferric chloride is 1：4.
In above-mentioned steps three, after the anhydrous ferric chloride is dissolved with 1% aqueous acetic acid, the pyrrole is added
Cough up-chitosan solution.
Brief description of the drawings
Fig. 1 be incubated overnight (>=10h) after, with the state diagram of the living cells of fluorescence labeling；
Fig. 2 be incubated overnight (>=10h) after, do not carry out the cell state figure of fluorescence labeling；
Fig. 3 is the electron microscope of 1000 times of amplification；
Fig. 4 is the electron microscope of 5000 times of amplification.
Specific embodiment
First, with reference to specific embodiment, the invention will be further described.
Embodiment 1：
A kind of preparation method of polypyrrole-shitosan composite conductive thin film, comprises the following steps：
Step one, the acetum for preparing shitosan by the following method；
The acetum of the aqueous acetic acid of configuration 1%, then the shitosan with aqueous acetic acid configuration 2% first, most
Afterwards by the acetum of the shitosan in 50 DEG C, with magnetic stirrer until dissolving.
Step 2, after the acetum of the shitosan is cooled to room temperature, under the conditions of lucifuge, M (pyrroles in mass ratio
Cough up)：M (shitosan)=1：4 ratio, to pyrroles is added in the acetum of shitosan, stirs 1.5h, obtains pyrroles-shitosan
Solution.
Step 3, in molar ratio n (pyrroles)：N (anhydrous ferric chloride)=1：3 ratio, to the pyrroles-chitosan solution
Middle addition anhydrous ferric chloride stirring 20h, oxidation obtains into film liquid.
Step 4, take described into film liquid, be added dropwise in the culture dish with sheet glass, the culture dish is placed on sol evenning machine
Middle masking.
Embodiment 2：
A kind of preparation method of polypyrrole-shitosan composite conductive thin film, comprises the following steps：
Step one, the acetum for preparing shitosan by the following method；
The acetum of the aqueous acetic acid of configuration 1%, then the shitosan with aqueous acetic acid configuration 2% first, most
Afterwards by the acetum of the shitosan in 50 DEG C, with magnetic stirrer until dissolving.
Step 2, after the acetum of the shitosan is cooled to room temperature, under the conditions of lucifuge, M (pyrroles in mass ratio
Cough up)：M (shitosan)=1：5 ratio, to pyrroles is added in the acetum of shitosan, stirs 2h, obtains pyrroles-shitosan molten
Liquid.
Step 3, in molar ratio n (pyrroles)：N (anhydrous ferric chloride)=1：4 ratio, to the pyrroles-chitosan solution
Middle addition anhydrous ferric chloride stirring 24h, oxidation obtains into film liquid.
Step 4, take described into film liquid, be added dropwise in the culture dish with sheet glass, the culture dish is placed on sol evenning machine
Middle masking.
Embodiment 3：
The present embodiment is with the difference of embodiment 1 and embodiment 2：
In the step 2, M in mass ratio (pyrroles)：M (shitosan)=1：6 ratio, to the acetum of shitosan
Middle addition pyrroles, stirs 3h, obtains pyrroles-chitosan solution.
In the step 3, n (pyrroles) in molar ratio：N (anhydrous ferric chloride)=1：5 ratio, it is poly- to the pyrroles-shell
Anhydrous ferric chloride stirring 30h is added in sugar juice, oxidation obtains into film liquid.
Embodiment 4：
A kind of preparation method of polypyrrole-shitosan composite conductive thin film, comprises the following steps：
First, the aqueous acetic acid of configuration 1%；
Secondly, in conical flask, 15ml, 1% aqueous acetic acid dissolving 0.4g shitosans, in the bar that temperature is 50 DEG C are taken
Under part, with magnetic stirrer, until dissolving, is cooled to room temperature；
Again, under the conditions of lucifuge, to 83ul is added in conical flask, density is the pyrroles of 0.96g/ml, stirs 2h；
Finally, take 5ml, 1% aqueous acetic acid fully dissolves 0.78g anhydrous ferric chlorides, by ferric chloride solution by
It is added dropwise in conical flask,
24h is kept stirring for, film liquid is obtained into.
Step 4, take described into film liquid, be added dropwise in the culture dish with sheet glass, the culture dish is placed on sol evenning machine
Middle masking.
Sol evenning machine filming technology is as follows：
A small amount of solution is drawn, is added dropwise in the culture dish with sheet glass, whole culture dish is placed in sol evenning machine, if
Put t1=9s, t2=30s；V=200r/s, v2=3200r/s, the vacuum pumping time are about half a minute, are pressed out after terminating
Beginning button, liquid will be unrolled evenly to form a film.The operation can be by adjusting time of the consumption of liquid, sol evenning machine
And the experimental variable such as rotating speed controls the thickness of film, the thickness of requirement of experiment film is thin as much as possible, film is in room temperature (25 DEG C~30
DEG C) under the conditions of spontaneously dry overnight.Using alternately cleaning 3 times of preceding use absolute ethyl alcohol and deionized water, immersion 5 minutes, obtain every time
To experiment conductive film, you can for cell culture.
2nd, with reference to experiment and accompanying drawing, the invention will be further described.
The experiment conductive film that embodiment 4 is obtained is tested, it is as a result as follows：
The measure of 2.1 electrical conductivity, the electrical conductivity for using four probe method to measure experiment conductive film is 0.51mS/cm.Explanation
Experiment conductive film has good electric conductivity.
2.2 biocompatibilities are determined, and to test conductive film as the carrier of cell growth, (＞ 10h) overnight culture is seen
Cell state is examined, as a result as depicted in figs. 1 and 2；
Fig. 1 be incubated overnight after, with the state diagram of the living cells of fluorescence labeling；
Fig. 2 be incubated overnight after, do not carry out the cell state figure of fluorescence labeling；
In Fig. 1, the profile of white transmission region and its surrounding is living cells；
In Fig. 2, dark strokes and its region as living cells for surrounding.
It is good cell growing state on experiment conductive film to be can be seen that from Fig. 1 and Fig. 2, after the long period, cell
Still there is survival rate higher, illustrate that experiment conductive film has good biocompatibility.
2.3 electron microscopic observations test the surface state of conductive film, electron microscope when Fig. 3 is to amplify 1000 times, and Fig. 4 is amplification
Electron microscope at 5000 times.
From figs. 3 and 4 it can be seen that experiment conductive film surface is uniform, it is smooth.
Beneficial effect：The conductive composite film prepared using the inventive method has not only manufactured good micro-loop to fluorescence molecule
Border makes it be difficult to influence fluorescence lifetime, and with preferable ductility and transparency.Reached as the carrier of Imaging-PAM
The basic standards such as small, the good biocompatibility of nontoxic, nonirritant, challeng.Additionally, the present invention is to fluorescence imaging skill
Receptor protein in art increases to the quantum efficiency of fluorescent material.Prepare simply, raw material is cheap, it is living with good biology
Property and electric conductivity.As the growing carrier of the cell with fluorescence, experiment proves that cell is able to cell of surviving and observe
Internal fluorescence has continuous variation phenomenon.
Finally it should be noted that foregoing description is only the preferred embodiments of the present invention, the ordinary skill people of this area
Member on the premise of without prejudice to present inventive concept and claim, can make table as multiple types under enlightenment of the invention
Show, such conversion is each fallen within protection scope of the present invention.
Claims (8)
1. the preparation method of a kind of polypyrrole-shitosan composite conductive thin film, it is characterised in that comprise the following steps：
Step one, the acetum for preparing shitosan；
Step 2, under the conditions of lucifuge, M in mass ratio (pyrroles)：M (shitosan)=1：The ratio of 4-6, to the acetic acid of shitosan
Pyrroles is added in solution, pyrroles-chitosan solution is obtained；
Step 3, in molar ratio n (pyrroles)：N (anhydrous ferric chloride)=1：The ratio of 3-5, in the pyrroles-chitosan solution
Add anhydrous ferric chloride；
Step 4, masking.
2. the preparation method of polypyrrole according to claim 1-shitosan composite conductive thin film, it is characterised in that：It is described
In step one, the collocation method of the acetum of the shitosan is：1% aqueous acetic acid is configured first, then uses the acetic acid
The acetum of the shitosan of aqueous solution configuration 2%, finally stirs until dissolving the acetum of the shitosan in 50 DEG C.
3. the preparation method of polypyrrole according to claim 2-shitosan composite conductive thin film, it is characterised in that：It is described
In step 2, institute is added after the acetum of the shitosan is cooled to room temperature, then in acetum to the shitosan
Pyrroles is stated, 1.5-3h is stirred.
4. the preparation method of the polypyrrole according to claim 1,2 or 3-shitosan composite conductive thin film, it is characterised in that：
In the step 3, after adding anhydrous ferric chloride, 20-30h is stirred, oxidation obtains into film liquid.
5. the preparation method of polypyrrole according to claim 1-shitosan composite conductive thin film, it is characterised in that：It is described
In step 4, take described into film liquid, be added dropwise in the culture dish with sheet glass, the culture dish is placed in sol evenning machine and is made
Film.
6. the preparation method of polypyrrole according to claim 1-shitosan composite conductive thin film, it is characterised in that：It is described
In step 2, the mass ratio of the pyrroles and shitosan is 1：5.
7. the preparation method of polypyrrole according to claim 1-shitosan composite conductive thin film, it is characterised in that：It is described
In step 3, the mol ratio of the pyrroles and anhydrous ferric chloride is 1：4.
8. the preparation method of polypyrrole according to claim 2-shitosan composite conductive thin film, it is characterised in that：It is described
In step 3, after the anhydrous ferric chloride is dissolved with 1% aqueous acetic acid, the pyrroles-shitosan is added molten
Liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611169657.6A CN106750579A (en) | 2016-12-16 | 2016-12-16 | The preparation method of polypyrrole shitosan composite conductive thin film |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611169657.6A CN106750579A (en) | 2016-12-16 | 2016-12-16 | The preparation method of polypyrrole shitosan composite conductive thin film |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106750579A true CN106750579A (en) | 2017-05-31 |
Family
ID=58892295
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611169657.6A Pending CN106750579A (en) | 2016-12-16 | 2016-12-16 | The preparation method of polypyrrole shitosan composite conductive thin film |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106750579A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108721617A (en) * | 2018-05-23 | 2018-11-02 | 上海理工大学 | A kind of preparation method of multi-functional conjugated highpolymer gel based composites |
| CN109161529A (en) * | 2018-08-23 | 2019-01-08 | 长春理工大学 | A kind of film of poly pyrrole substrate preparation method for cell culture |
| CN112779784A (en) * | 2021-01-27 | 2021-05-11 | 南京信息工程大学 | Chitosan-polypyrrole conductive fiber with core-shell structure and preparation method thereof |
| CN112844058A (en) * | 2020-12-30 | 2021-05-28 | 河北工程大学 | Preparation method of single-side modified cation exchange membrane |
| CN112924316A (en) * | 2021-02-09 | 2021-06-08 | 中国石油大学(华东) | Humidity-sensitive film based on chitosan and polypyrrole and preparation method and application thereof |
| CN113930245A (en) * | 2021-11-16 | 2022-01-14 | 天津科技大学 | Environment-friendly Cr (VI) polluted soil remediation agent and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04253744A (en) * | 1991-02-04 | 1992-09-09 | Showa Denko Kk | Conductive polymeric material and its production |
| WO2014121378A1 (en) * | 2013-02-05 | 2014-08-14 | University Health Network | Conductive biomaterial for enhancement of conduction in vitro and in vivo |
| CN104151630A (en) * | 2014-08-08 | 2014-11-19 | 江汉大学 | Preparation method of chitosan-based composite material having electroactivity |
| CN105645523A (en) * | 2016-01-07 | 2016-06-08 | 西安建筑科技大学 | Method for preparing polypyrrole/chitosan composite electrode |
| CN105692812A (en) * | 2016-04-22 | 2016-06-22 | 西安建筑科技大学 | Method for applying polypyrrole/chitosan combined electrode to heavy metal ion adsorption |
| CN106057485A (en) * | 2016-08-19 | 2016-10-26 | 南京林业大学 | Method for preparing super capacitor aerogel electrode material |
-
2016
- 2016-12-16 CN CN201611169657.6A patent/CN106750579A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04253744A (en) * | 1991-02-04 | 1992-09-09 | Showa Denko Kk | Conductive polymeric material and its production |
| WO2014121378A1 (en) * | 2013-02-05 | 2014-08-14 | University Health Network | Conductive biomaterial for enhancement of conduction in vitro and in vivo |
| CN104151630A (en) * | 2014-08-08 | 2014-11-19 | 江汉大学 | Preparation method of chitosan-based composite material having electroactivity |
| CN105645523A (en) * | 2016-01-07 | 2016-06-08 | 西安建筑科技大学 | Method for preparing polypyrrole/chitosan composite electrode |
| CN105692812A (en) * | 2016-04-22 | 2016-06-22 | 西安建筑科技大学 | Method for applying polypyrrole/chitosan combined electrode to heavy metal ion adsorption |
| CN106057485A (en) * | 2016-08-19 | 2016-10-26 | 南京林业大学 | Method for preparing super capacitor aerogel electrode material |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108721617A (en) * | 2018-05-23 | 2018-11-02 | 上海理工大学 | A kind of preparation method of multi-functional conjugated highpolymer gel based composites |
| CN108721617B (en) * | 2018-05-23 | 2021-03-05 | 上海理工大学 | Preparation method of multifunctional conjugated polymer gel-based composite material |
| CN109161529A (en) * | 2018-08-23 | 2019-01-08 | 长春理工大学 | A kind of film of poly pyrrole substrate preparation method for cell culture |
| CN109161529B (en) * | 2018-08-23 | 2020-08-04 | 长春理工大学 | Preparation method of polypyrrole film substrate for cell culture |
| CN112844058A (en) * | 2020-12-30 | 2021-05-28 | 河北工程大学 | Preparation method of single-side modified cation exchange membrane |
| CN112779784A (en) * | 2021-01-27 | 2021-05-11 | 南京信息工程大学 | Chitosan-polypyrrole conductive fiber with core-shell structure and preparation method thereof |
| CN112779784B (en) * | 2021-01-27 | 2023-01-03 | 南京信息工程大学 | Chitosan-polypyrrole conductive fiber with core-shell structure and preparation method thereof |
| CN112924316A (en) * | 2021-02-09 | 2021-06-08 | 中国石油大学(华东) | Humidity-sensitive film based on chitosan and polypyrrole and preparation method and application thereof |
| CN113930245A (en) * | 2021-11-16 | 2022-01-14 | 天津科技大学 | Environment-friendly Cr (VI) polluted soil remediation agent and preparation method thereof |
| WO2023088292A1 (en) * | 2021-11-16 | 2023-05-25 | 天津科技大学 | Environment-friendly remediation agent for cr(vi)-contaminated soil, and preparation method therefor and use thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106750579A (en) | The preparation method of polypyrrole shitosan composite conductive thin film | |
| Tomczykowa et al. | Conducting polymers, hydrogels and their composites: Preparation, properties and bioapplications | |
| Song et al. | Chestnut-tannin-crosslinked, antibacterial, antifreezing, conductive organohydrogel as a strain sensor for motion monitoring, flexible keyboards, and velocity monitoring | |
| Li et al. | Electronic biopolymers: From molecular engineering to functional devices | |
| Lu et al. | Electroconductive hydrogels for biomedical applications | |
| Khan et al. | Nano-gold assisted highly conducting and biocompatible bacterial cellulose-PEDOT: PSS films for biology-device interface applications | |
| Miao et al. | Biocompatible lignin-containing hydrogels with self-adhesion, conductivity, UV shielding, and antioxidant activity as wearable sensors | |
| Hao et al. | Polypyrrole nanomaterials: Structure, preparation and application | |
| Truong et al. | In situ fabrication of electrically conducting bacterial cellulose-polyaniline-titanium-dioxide composites with the immobilization of Shewanella xiamenensis and its application as bioanode in microbial fuel cell | |
| Khati et al. | 3D bioprinting of multi-material decellularized liver matrix hydrogel at physiological temperatures | |
| Visan et al. | Long-term evaluation of dip-coated pcl-blend-peg coatings in simulated conditions | |
| Duarte et al. | In situ carbon felt anode modification via codeveloping Saccharomyces cerevisiae living-template titanium dioxide nanoclusters in a yeast-based microbial fuel cell | |
| CN105355450A (en) | Preparation method and application of nitrogen-doped carbon fiber/nitrogen-doped graphene/bacterial cellulose membrane material | |
| Zhu et al. | Electroactive oxidized alginate/gelatin/MXene (Ti3C2Tx) composite hydrogel with improved biocompatibility and self-healing property | |
| Lu et al. | Cost-effective three dimensional Ag/polymer dyes/graphene-carbon spheres hybrids for high performance nonenzymatic sensor and its application in living cell H2O2 detection | |
| Gebeyehu et al. | Cellulosic-based conductive hydrogels for electro-active tissues: A review summary | |
| Tohidi et al. | An electroconductive, thermosensitive, and injectable chitosan/pluronic/gold-decorated cellulose nanofiber hydrogel as an efficient carrier for regeneration of cardiac tissue | |
| Pan et al. | Covalently injectable chitosan/chondroitin sulfate hydrogel integrated gelatin/heparin microspheres for soft tissue engineering | |
| Kwon et al. | Stability of carbon nanotube yarn biofuel cell in human body fluid | |
| Skorupa et al. | Dopant-dependent electrical and biological functionality of pedot in bioelectronics | |
| Li et al. | Engineering ionic dough with a deep eutectic solvent: from a traditional dough figurine to flexible electronics | |
| Lee et al. | Recent advances using MXenes in biomedical applications | |
| Shen et al. | Peroxidase-like N-doped carbon nanotubes loaded with NiCo-layered double-hydroxide nanoflowers for dual-mode ultrasensitive and real-time detection of H2O2 secreted from cancer cells | |
| Yang et al. | Au and Pt nanoparticles grown on flexible carbon fiber cloth supports decorated with cerium metal organic frameworks for the real-time detection of H2O2 in live cancer tissue | |
| Saez et al. | A 3D bioelectrical interface to assess colorectal cancer progression in vitro |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20170531 |
|
| RJ01 | Rejection of invention patent application after publication |