CN106746942A - A kind of metro engineering concrete that can continue selfreparing comprising microbial capsules - Google Patents

A kind of metro engineering concrete that can continue selfreparing comprising microbial capsules Download PDF

Info

Publication number
CN106746942A
CN106746942A CN201611006062.9A CN201611006062A CN106746942A CN 106746942 A CN106746942 A CN 106746942A CN 201611006062 A CN201611006062 A CN 201611006062A CN 106746942 A CN106746942 A CN 106746942A
Authority
CN
China
Prior art keywords
parts
concrete
microbial capsules
cement
selfreparing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201611006062.9A
Other languages
Chinese (zh)
Other versions
CN106746942B (en
Inventor
唐禄博
张华玮
赵慷慨
林凡通
李壮壮
王丽丽
王鹏飞
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
University of Jinan
Original Assignee
University of Jinan
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by University of Jinan filed Critical University of Jinan
Priority to CN201611006062.9A priority Critical patent/CN106746942B/en
Priority to CN201810653734.8A priority patent/CN108947341B/en
Priority to CN201810653733.3A priority patent/CN108947340B/en
Publication of CN106746942A publication Critical patent/CN106746942A/en
Application granted granted Critical
Publication of CN106746942B publication Critical patent/CN106746942B/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
    • C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
    • C04B28/00—Compositions of mortars, concrete or artificial stone, containing inorganic binders or the reaction product of an inorganic and an organic binder, e.g. polycarboxylate cements
    • C—CHEMISTRY; METALLURGY
    • C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
    • C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
    • C04B20/00—Use of materials as fillers for mortars, concrete or artificial stone according to more than one of groups C04B14/00 - C04B18/00 and characterised by shape or grain distribution; Treatment of materials according to more than one of the groups C04B14/00 - C04B18/00 specially adapted to enhance their filling properties in mortars, concrete or artificial stone; Expanding or defibrillating materials
    • C04B20/10—Coating or impregnating
    • C04B20/12—Multiple coating or impregnating
    • C—CHEMISTRY; METALLURGY
    • C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
    • C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
    • C04B24/00—Use of organic materials as active ingredients for mortars, concrete or artificial stone, e.g. plasticisers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
    • C—CHEMISTRY; METALLURGY
    • C04—CEMENTS; CONCRETE; ARTIFICIAL STONE; CERAMICS; REFRACTORIES
    • C04B—LIME, MAGNESIA; SLAG; CEMENTS; COMPOSITIONS THEREOF, e.g. MORTARS, CONCRETE OR LIKE BUILDING MATERIALS; ARTIFICIAL STONE; CERAMICS; REFRACTORIES; TREATMENT OF NATURAL STONE
    • C04B2201/00—Mortars, concrete or artificial stone characterised by specific physical values
    • C04B2201/50—Mortars, concrete or artificial stone characterised by specific physical values for the mechanical strength

Landscapes

  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Ceramic Engineering (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Structural Engineering (AREA)
  • Materials Engineering (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Inorganic Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Curing Cements, Concrete, And Artificial Stone (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Processing Of Solid Wastes (AREA)

Abstract

The invention discloses a kind of metro engineering concrete that can continue selfreparing comprising microbial capsules, it is related to concrete recovery technique field, the concrete is C30 concrete, the raw material comprising following weight portion：266 parts of cement, 114 parts of flyash, 718 parts of sand, 592 parts of fine stone, 484 parts of small crushed stone, 157 parts of water, 33 parts of HLC anti-crack and anti-seepages agent, 15 20 parts of microbial capsules.Concrete of the invention can in a long time replace artificial solution underground engineering seepage problem, and it repairs power preferably, up to 99%, therefore, concrete of the invention has larger economic benefit and market potential.

Description

A kind of metro engineering concrete that can continue selfreparing comprising microbial capsules
Technical field
The present invention relates to concrete recovery technique field, more particularly to one kind can continue selfreparing comprising microbial capsules Metro engineering concrete.
Background technology
The treatment of active service concrete structure problem of Cracking, is the universal technical barrier of whole field of civil engineering.The world is each There is the limit value of crack allowance width in state, and such as U.S. and Zelanian technical standard crack allowance width (dry ring for 0.4mm Under border), China is 0.2~0.3mm.The appearance in crack have impact on the security and applicability of structure.If splitting occurs in underground engineering Seam, it will produce infiltration, have a strong impact on the use of underground engineering；And after crack occurs in beam, post, wall of office building, house etc., no Architectural appearance and life-span are only influenceed, the safety of life and property of people can be also threatened.Accordingly, it would be desirable to take suitable engineering skill Art means, repair process is carried out to band cracked concrete.At present, conventional method has surface sealing method, leak stopping method and structure to mend Method for strengthening etc., but surface sealing method by force cannot go deep into inside crack, be not suitable for the crack for having obvious hydraulic pressure；The conventional filling of leak stopping method Slurry, caulking material are the organic material such as epoxy resin, polyurethane, and the thermal coefficient of expansion from cement-based material is different, compatibility compared with Difference, in addition, organic chemical is mostly volatile, the gas for discharging is harmful to human body and environment；Structural strengthening and repairing method is grasped Make complicated, it is relatively costly.
Tosca (MICP) technology of microorganism induction is repaiied as a kind of brand-new technology standby application in concrete Bowl spares.Microorganism induction bonding refers to the gelling property of the calcium carbonate using microorganism induction generation, improves cement base class material Intensity and endurance quality.Mainly there are three kinds of schemes:1) crack is repaired, will bacterium solution, culture medium, filler aggregate (sand, poly- ammonia Ester, Silica hydrogel etc.) filling crack, acted on by MICP, packing material is filled crack with the calcium carbonate crystal of generation, part Recover the intensity of concrete and improve durability, the defect of this scheme is still to need later stage manual repair, is taken time and effort；2) it is micro- Bacterium solution is added during biological concrete, i.e. mixing concrete, to produce MICP in inside concrete hole, in compression strength not Improve tensile strength and durability in the case of reduction, the defect of this scheme is that the alkalescence of concrete can influence the activity of strain, It is set to be chronically at resting state, the existence of spore and developmental potency can gradually drop as the age of concrete elongated space diminishes Low, life span is less than four months, and after death microbes decomposition, has an impact to concrete strength；3) selfreparing or self-healing are mixed Solidifying soil, is first made dry microbial capsules by bacterium and culture medium, is mixed when concrete is mixed and stirred, and bacterium is initially located in dormancy State, once concrete cracking, oxygen and moisture enter, bacterium can recover metabolic function, and breathing produces carbon dioxide, Carbon dioxide generates calcium carbonate with the calcium ion reaction in cement-based material in moist environment, with this filling cracks, the method Defect be, because capsule is single use, for ensure have crackle when, capsule ftractures therewith, therefore capsule wall is thin, start A large amount of microbial capsules ruptures occur when admixing concrete, repair is not had；Even if in addition, microbial capsules Without rupture, microbial capsules have met water activation when concrete is admixed, and substantial amounts of water can make during mixed concrete Microorganisms effect loses follow-up repair, such as Application No. until exhausting the intracapsular culture medium of microbiological gum The patent of " 201210580233 parts of .4 " disclose " a kind of complex capsule underground structure concrete of built-in aerobic microorganism from Repair system, including concrete substrate, are provided with microorganism complex capsule, the microorganism complex capsule in the concrete substrate Inside it is coated with aerobic microorganism and is adapted to the culture medium of the aerobic growth of microorganism." can be mixed without additional culture medium Solidifying soil can realize intelligent reparation.Its mechanism is crack penetration complex capsule, discharges microorganism and metabolism generation occurs Carbon dioxide reacts into calcium carbonate to repair crack with calcium ion, and the method has following defect：Firstth, the culture in complex capsule Base and Microbiological release can produce influence after going out on the intensity of concrete；Secondth, capsule is used only once, it is impossible to mixed The crackle of solidifying soil same position carries out lasting reparation；3rd, outer surface is round and smooth structure, with the caking property of concrete compared with Easily it is flushed away after difference, and water inflow, and repair cannot be played.In consideration of it, one kind does not excite micro- life when stirring is started Thing is active, the sustainable research for repairing concrete capsule is in the urgent need to address.
The content of the invention
In view of this, the present invention provides one kind and can keep activity, superior with the caking property of concrete and can repeatedly hold The continuous metro engineering concrete that can continue selfreparing comprising microbial capsules for repairing concrete cracks, mainly for easily oozing Water and the artificial underground engineering for repairing difficulty.
In order to realize foregoing invention purpose, the present invention provides following technical scheme：
A kind of metro engineering concrete that can continue selfreparing comprising microbial capsules, the concrete is C30 coagulations Soil, the raw material comprising following weight portion：266 parts of cement, 114 parts of flyash, 718 parts of sand, 592 parts of fine stone, small crushed stone 484 Part, 157 parts of water, 33 parts, microbial capsules 15-20 parts of HLC anti-crack and anti-seepages agent.
The present invention also has following additional technical feature：
Preferably, secondary immersion in alcohol of the microbial capsules before with concrete-agitating through 90-100%wt 20-50min。
Preferably, the microbial capsules include sandwich layer, protective layer, outer layer；The sandwich layer is to be adsorbed with aerobic basophilic bud Spore bacillus and the macroporous absorbent resin of correspondence culture medium dry powder, a diameter of 0.1-1mm, the protective layer material is cement, thickness It is 0.1-1mm, the cladding material is cement and calcium chloride mixed layer, and thickness is 0.1-1mm.
Preferably, the preparation method of the microbial capsules is comprised the following steps：
Step one, the aerobic Alkaliphilic bacillus of culture, obtain bacterium mud after centrifugation；
Step 2, the anhydrous aerobic Alkaliphilic bacillus culture medium of configuration；
Step 3, culture medium and bacterium mud are added to the water it are uniformly mixing to obtain mixed liquor, the quality of the water is culture medium With 1-3 times of bacterium mud gross mass, the amount of the culture medium is at least to make bacterium mud eubolism 72 hours；
After adding macroporous absorbent resin in step 4, the mixed liquor obtained to step 3, carried out with the speed of 250r/min Stirring 20-30min, is collected by filtration macroporous absorbent resin, water content is dried in 20-30 DEG C less than 5%, the quality of the mixed liquor It is 10-20 times of the macroporous absorbent resin；
Step 5, the macroporous absorbent resin after drying is placed in the alcohol of 90-100%wt and once soaks 20-50min Afterwards, cement liquid is uniformly sprayed on its surface, is dried in 20-30 DEG C, again spray cement and calcium chloride mixed liquor, in 20-30 DEG C Drying, that is, obtain microbial capsules, and the mass fraction of the cement liquid is 20-30%, the cement and calcium chloride mixed liquor Mass fraction is 20-30%.
Preferably, the cement is 2-3 with the mass ratio of calcium chloride with cement in calcium chloride mixed layer：1.
Preferably, the aerobic Alkaliphilic bacillus are lysine bacillus or Bacillus cercus.
Preferably, the macroreticular resin is drift Lay spy A510.
Preferably, the fine stone particle diameter is 3mm, and small crushed stone particle diameter is 5-16mm.
Compared to the prior art the present invention, the advantage is that：
Mainly for underground engineering seepage problem, in the case of without infiltration, microbial capsules are dry to concrete of the invention State, culture medium is dry powder-shaped, and in a dormant state, after seeping water, microbial capsules will not ftracture strain, protective layer and outer Layer has infiltration, and permeation functions, moisture and oxygen are penetrated into microbial capsules, and activation strain carries out metabolism generation carbon dioxide, Carbon dioxide generates precipitation of calcium carbonate filling cracks with the calcium ion reaction in concrete, and the addition of outer layer calcium chloride can accelerate And increasing generation and the precipitation of calcium carbonate, crack once replenishes, and the strain in microbial capsules is isolated oxygen again and water enters Enter resting state, thus, microbial capsules repeatedly lasting can carry out concrete reparation；
In addition, microbial capsules separate strain with concrete layer, strain can be made to be influenceed from high alkalinity, improve strain steady Qualitative, also prevent strain and after death decompose to produce concrete influences；
Again, the sandwich layer of microbial capsules is macroporous absorbent resin, with macroreticular structure and than larger specific surface Product, the adsorbance to strain and culture medium is big, it is ensured that strain plays the number of times of repair, also, macroreticular resin has Preferable compression strength, does not interfere with the stability of concrete, and the outer layer of microbial capsules is combined for cement composition with concrete Stabilization, caking property is strong, will not fall off, or is swept away by water, to ensure when concrete cracking, to play a role in time；
Finally, microbial capsules are carried out before mixing with concrete alcohol-pickled, are at resting state, prevent by Water is activated, and influences the performance of follow-up repair, and this technical characterstic does not occur in the prior art, with pioneering meaning Justice.
Specific embodiment
Following discloses some embodiments of the present invention, those skilled in the art can be suitably modified work according to present disclosure Skill parameter is realized.In particular, all similar replacements and change are for a person skilled in the art aobvious and easy See, they are considered as being included in the present invention.The method of the present invention and application are described by preferred embodiment, Related personnel substantially can not depart from present invention, spirit and scope to method described herein and application be modified or Suitably change with combining to realize and apply the technology of the present invention.
Embodiment 1
Prepare microbial capsules：
Step one, culture lysine bacillus, obtain bacterium mud after centrifugation, bacterium mud concentration is 6 × 109Individual/ml；
Step 2, the anhydrous lysine bacillus of configuration, tryptone 510g, soy peptone 90g, glucose 75, NaCl150g；Dipotassium hydrogen phosphate 25g；
Step 3, all culture mediums that step 2 is configured and 0.1ml bacterium muds are uniformly mixing to obtain in adding 1200ml water Mixed liquor；
After adding 100g macroporous absorbent resins in step 4, the mixed liquor obtained to step 3, with the speed of 250r/min 30min is stirred, macroporous absorbent resin is collected by filtration, water content is dried in 20 DEG C less than 5%；
Step 5, the macroporous absorbent resin after drying is placed in the alcohol of 90%wt carry out for the first time immersion 30min after, Mass fraction is uniformly sprayed for 20% cement liquid on its surface, makes its thickness for 0.3mm, dried in 30 DEG C, again spray cement With calcium chloride according to mass ratio 2.5：The mixed liquor of 1 configuration, the mass fraction of mixed liquor is 20%, makes its thickness for 0.8mm, in 30 DEG C of drying, that is, obtain microbial capsules.
Prepare concrete：266 parts of cement, 114 parts of flyash, 718 parts of sand, 592 parts of fine stone, 484 parts of small crushed stone, water 157 parts, 33 parts of HLC anti-crack and anti-seepages agent, 15 parts of microbial capsules (being placed in the alcohol of 90%wt second immersion 20min) will Microbial capsules are poured into water fully dispersed uniform, then use conventional concrete collocation method preparation.
Comparative example 1 compared with Example 1, without microbial capsules, other component all sames.
Embodiment 2
Prepare microbial capsules：
Step one, culture lysine bacillus or Bacillus cercus, obtain bacterium mud after centrifugation, and bacterium mud concentration is 6 × 109Individual/ml；
Step 2, the anhydrous aerobic Alkaliphilic bacillus culture medium of configuration, tryptone 510g, soy peptone 90g, Portugal Grape sugar 75, NaCl150g；Dipotassium hydrogen phosphate 25g；
Step 3, all culture mediums that step 2 is configured and 0.1ml bacterium muds are uniformly mixing to obtain in adding 1500ml water Mixed liquor；
After adding 100g macroporous absorbent resins in step 4, the mixed liquor obtained to step 3, with the speed of 250r/min 20min is stirred, macroporous absorbent resin is collected by filtration, water content is dried in 30 DEG C less than 5%；
Step 5, the macroporous absorbent resin after drying be placed in once soak 50min in the alcohol of 100%wt after, at it Mass fraction is uniformly sprayed for 25% cement liquid in surface, makes its thickness for 0.8mm, is dried in 25 DEG C, again spray cement and chlorine Change calcium according to mass ratio 3：The mixed liquor of 1 configuration, makes its thickness for 0.7mm, and the mass fraction of mixed liquor is 30%, in 25 DEG C of bakings It is dry, that is, obtain microbial capsules.
Prepare concrete：266 parts of cement, 114 parts of flyash, 718 parts of sand, 592 parts of fine stone, 484 parts of small crushed stone, water 157 parts, 33 parts of HLC anti-crack and anti-seepages agent, 18 parts of microbial capsules (being placed in the alcohol of 90%wt second immersion 30min) will Microbial capsules are poured into water fully dispersed uniform, then use conventional concrete collocation method preparation.
Embodiment 3
Prepare microbial capsules：
Step one, culture Bacillus cercus, obtain bacterium mud after centrifugation, bacterium mud concentration is 4 × 109Individual/ml；
Step 2, the anhydrous M.S.P culture mediums of configuration, NaNO39g, K2HPO43g, KCI1.5g, MgSO41.5g, FeSO4, 0.03g, sucrose 90g；
It is uniformly mixing to obtain in step 3, all culture mediums that step 2 is configured and 0.1ml bacterium muds addition 180ml water mixed Close liquid；
After adding 15g macroporous absorbent resins in step 4, the mixed liquor obtained to step 3, entered with the speed of 250r/min Row stirring 30min, is collected by filtration macroporous absorbent resin, and water content is dried in 30 DEG C less than 5%；
Step 5, the macroporous absorbent resin after drying be placed in once soak 40min in the alcohol of 100%wt after, at it Mass fraction is uniformly sprayed for 25% cement liquid in surface, makes its thickness for 0.5mm, is dried in 30 DEG C of drying bakers, sprays again Cement is 2 with calcium chloride mass ratio：1 mixed liquor, the mass fraction of mixed liquor is 25%, makes its thickness for 0.5mm, in 30 DEG C Drying, that is, obtain microbial capsules.
Prepare concrete：266 parts of cement, 114 parts of flyash, 718 parts of sand, 592 parts of fine stone, 484 parts of small crushed stone, water 157 parts, 33 parts of HLC anti-crack and anti-seepages agent, 20 parts of microbial capsules (being placed in the alcohol of 90%wt second immersion 40min) will Microbial capsules are poured into water fully dispersed uniform, then use conventional concrete collocation method preparation.
The effect detection of embodiment 4
Prepare test block：The concrete of embodiment 1-3 and comparative example is respectively prepared the rectangle test block of 4 × 10cm, and Carry out the detection of following items；
Microbial capsules spoilage is detected：After just press-in test block, part test block is taken, check that microbial capsules are damaged Rate；
Compression strength is detected：Test block natural curing surveys every group of compression strength in one month afterwards；
Concrete repairs situation observation：Pressure destruction is carried out to test block, is allowed to crack, it is 95% to be placed in humidity, temperature Spend for 24 hours in 15 DEG C of environment, cutting afterwards is decomposed, and SEM checks its reparation situation；
Concrete repairs number of times observation：Multiple pressure destruction is carried out to test block, is allowed to crack, being placed in humidity is 95%, temperature is that 24 hours in 15 DEG C of environment, cutting afterwards is decomposed, and SEM checks its reparation situation, until crack forming mechanism volume is low In 50%；
Microbial capsules repair number of times detection：It is 95% that microbial capsules are placed in into humidity, and temperature is 24 in 15 DEG C of environment Hour,
30 DEG C of drying are taken out, sees whether that surface has crystal to produce, repeatedly, until being produced without crystal.
Testing result is shown in Table 1：
The effect detection of the microbial capsules of table 1
The experiment condition for detecting above, temperature and humidity are imitation underground environment, of the invention micro- to truly reflect The repairing effect of biological capsule, from table 1, microbial capsules of the invention are added and have no effect on the solidifying native resistance to compression of of coagulation itself Intensity, and the repair rate of concrete 50%, up to 4 times, in actual applications, concrete of the invention can replace in a long time It is artificial to solve underground engineering seepage problem, and it repairs power preferably, up to 99%, therefore, concrete of the invention has larger Economic benefit and market potential.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should It is considered as protection scope of the present invention.

Claims (7)

1. a kind of metro engineering concrete that can continue selfreparing comprising microbial capsules, it is characterised in that the concrete It is C30 concrete, the raw material comprising following weight portion：266 parts of cement, 114 parts of flyash, 718 parts of sand, 592 parts of fine stone is small 484 parts of rubble, 157 parts of water, 33 parts, microbial capsules 15-20 parts of HLC anti-crack and anti-seepages agent.
2. the metro engineering concrete that selfreparing can be continued comprising microbial capsules according to claim 1, its feature It is, secondary immersion 20-50min in alcohol of the microbial capsules before with concrete-agitating through 90-100%wt.
3. the metro engineering concrete that selfreparing can be continued comprising microbial capsules according to claim 1, its feature It is that the microbial capsules include sandwich layer, protective layer, outer layer；The sandwich layer is to be adsorbed with aerobic Alkaliphilic bacillus and right The macroporous absorbent resin of culture medium dry powder is answered, a diameter of 0.1-1mm, the protective layer material is cement, and thickness is 0.1-1mm, The cladding material is cement and calcium chloride mixed layer, and thickness is 0.1-1mm.
4. the metro engineering concrete that selfreparing can be continued comprising microbial capsules according to claim 1, its feature It is that the preparation method of the microbial capsules is comprised the following steps：
Step one, the aerobic Alkaliphilic bacillus of culture, obtain bacterium mud after centrifugation；
Step 2, the anhydrous aerobic Alkaliphilic bacillus culture medium of configuration；
Step 3, culture medium and bacterium mud are added to the water it are uniformly mixing to obtain mixed liquor, the quality of the water is culture medium and bacterium 1-3 times of mud gross mass, the amount of the culture medium is at least to make bacterium mud eubolism 72 hours；
After adding macroporous absorbent resin in step 4, the mixed liquor obtained to step 3, it is stirred with the speed of 250r/min 20-30min, is collected by filtration macroporous absorbent resin, and water content is dried in 20-30 DEG C less than 5%, and the quality of the mixed liquor is institute State macroporous absorbent resin 10-20 times；
Step 5, the macroporous absorbent resin after drying be placed in once soak 20-50min in the alcohol of 90-100%wt after, at it Cement liquid is uniformly sprayed on surface, and in 20-30 DEG C of drying, spray cement and calcium chloride mixed liquor, dry, i.e., in 20-30 DEG C again Microbial capsules are obtained, the mass fraction of the cement liquid is the mass fraction of 20-30%, the cement and calcium chloride mixed liquor It is 20-30%.
5. the metro engineering concrete that can continue selfreparing comprising microbial capsules according to claim 3 or 4, it is special Levy and be, the cement is 2-3 with the mass ratio of calcium chloride with cement in calcium chloride mixed layer：1.
6. the metro engineering concrete that selfreparing can be continued comprising microbial capsules according to claim 5, its feature It is that the aerobic Alkaliphilic bacillus are lysine bacillus or Bacillus cercus.
7. the metro engineering concrete that selfreparing can be continued comprising microbial capsules according to claim 1, its feature It is that the macroreticular resin is drift Lay spy A510.
CN201611006062.9A 2016-11-16 2016-11-16 A kind of metro engineering concrete that can continue selfreparing comprising microbial capsules Expired - Fee Related CN106746942B (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
CN201611006062.9A CN106746942B (en) 2016-11-16 2016-11-16 A kind of metro engineering concrete that can continue selfreparing comprising microbial capsules
CN201810653734.8A CN108947341B (en) 2016-11-16 2016-11-16 Microbial capsule capable of continuously repairing subway engineering concrete
CN201810653733.3A CN108947340B (en) 2016-11-16 2016-11-16 Microbial capsule capable of continuously repairing subway engineering concrete

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201611006062.9A CN106746942B (en) 2016-11-16 2016-11-16 A kind of metro engineering concrete that can continue selfreparing comprising microbial capsules

Related Child Applications (2)

Application Number Title Priority Date Filing Date
CN201810653733.3A Division CN108947340B (en) 2016-11-16 2016-11-16 Microbial capsule capable of continuously repairing subway engineering concrete
CN201810653734.8A Division CN108947341B (en) 2016-11-16 2016-11-16 Microbial capsule capable of continuously repairing subway engineering concrete

Publications (2)

Publication Number Publication Date
CN106746942A true CN106746942A (en) 2017-05-31
CN106746942B CN106746942B (en) 2018-08-28

Family

ID=58969620

Family Applications (3)

Application Number Title Priority Date Filing Date
CN201611006062.9A Expired - Fee Related CN106746942B (en) 2016-11-16 2016-11-16 A kind of metro engineering concrete that can continue selfreparing comprising microbial capsules
CN201810653734.8A Active CN108947341B (en) 2016-11-16 2016-11-16 Microbial capsule capable of continuously repairing subway engineering concrete
CN201810653733.3A Expired - Fee Related CN108947340B (en) 2016-11-16 2016-11-16 Microbial capsule capable of continuously repairing subway engineering concrete

Family Applications After (2)

Application Number Title Priority Date Filing Date
CN201810653734.8A Active CN108947341B (en) 2016-11-16 2016-11-16 Microbial capsule capable of continuously repairing subway engineering concrete
CN201810653733.3A Expired - Fee Related CN108947340B (en) 2016-11-16 2016-11-16 Microbial capsule capable of continuously repairing subway engineering concrete

Country Status (1)

Country Link
CN (3) CN106746942B (en)

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107975385A (en) * 2017-10-16 2018-05-01 山东科技大学 A kind of coal mine roadway anchoring whitewashing microorganism self-healing system and its construction method
CN108558260A (en) * 2018-05-24 2018-09-21 中交四航工程研究院有限公司 A kind of self union concrete complex enzyme microencapsulation material and preparation method thereof
CN108947415A (en) * 2018-09-27 2018-12-07 无锡爱路建设有限公司 A kind of preparation method of high-performance road surface repair materials
CN108947308A (en) * 2018-07-22 2018-12-07 黑龙江工程学院 A kind of concrete external doped dose of permanent seal cooling
CN109231906A (en) * 2018-09-29 2019-01-18 佛山齐安建筑科技有限公司 A kind of preparation method of resistance to compression self-repair concrete
CN110386794A (en) * 2019-08-28 2019-10-29 沈华初 A kind of self-reinforcing building concrete and preparation method
CN110776270A (en) * 2019-11-06 2020-02-11 山东大学 Composite structure self-repairing microcapsule particle and preparation method and application thereof
CN111205838A (en) * 2020-02-26 2020-05-29 西南石油大学 Composite microcapsule for fractured rock mass solidification seepage prevention and preparation method thereof
WO2021008341A1 (en) * 2019-07-18 2021-01-21 华南理工大学 Cement-based material crack self-repairing agent capable of curing corrosive sea water ions, and preparation method therefor and application thereof
CN114349388A (en) * 2022-01-19 2022-04-15 郑州大学 Water-awakening self-healing microbial capsule and waterproof material prepared from same
WO2022236998A1 (en) * 2021-05-08 2022-11-17 东南大学 Microbial self-repair agent having viscous product and application thereof
CN116041019A (en) * 2023-01-17 2023-05-02 中建三局绿色产业投资有限公司 High-crack-resistance corrosion-resistance ultra-high-performance concrete and preparation method thereof

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110054444A (en) * 2019-04-30 2019-07-26 东南大学 It is a kind of suitable for the cement-based material self repairing agent of low temperature environment and its application
CN110282903B (en) * 2019-07-26 2021-08-24 深圳大学 Microcapsule for repairing concrete cracks and preparation method thereof
CN117136173A (en) * 2021-04-12 2023-11-28 南洋理工大学 Functional gradient bacterial capsule for concrete self-repairing

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102432242A (en) * 2011-10-08 2012-05-02 中铁十二局集团有限公司 Self-compacting concrete for high-speed railway turnout area slab ballastless track filling layer
CN103043937A (en) * 2012-12-27 2013-04-17 同济大学 Compound capsule underground structure concrete self-repairing system provided with aerobic microorganisms inside
KR101351382B1 (en) * 2013-08-19 2014-01-24 (주)평창건설 Concrete repairing method using self-healing repair mortar composite
CN103739254A (en) * 2013-12-18 2014-04-23 青岛青建新型材料有限公司 Underground anti-cracking and impermeable concrete with strength grade no more than C50 and preparation method thereof

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101270369A (en) * 2008-05-06 2008-09-24 清华大学 Microorganism cause concrete or concrete, producing method and application thereof
CN101289299A (en) * 2008-05-20 2008-10-22 深圳大学 Self-repair concrete using polyurea resin high molecule microcapsule and method of manufacture
GB201303690D0 (en) * 2013-03-01 2013-04-17 Devan Chemicals Nv Microcapsules and contrete containing the same
CN103342484B (en) * 2013-07-18 2014-09-03 东南大学 Method for repairing crack of cement-based material
CN106082767B (en) * 2016-06-06 2018-01-09 重庆三峡学院 A kind of string loads the self-repairing cement-base material of microorganism

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102432242A (en) * 2011-10-08 2012-05-02 中铁十二局集团有限公司 Self-compacting concrete for high-speed railway turnout area slab ballastless track filling layer
CN103043937A (en) * 2012-12-27 2013-04-17 同济大学 Compound capsule underground structure concrete self-repairing system provided with aerobic microorganisms inside
KR101351382B1 (en) * 2013-08-19 2014-01-24 (주)평창건설 Concrete repairing method using self-healing repair mortar composite
CN103739254A (en) * 2013-12-18 2014-04-23 青岛青建新型材料有限公司 Underground anti-cracking and impermeable concrete with strength grade no more than C50 and preparation method thereof

Cited By (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107975385A (en) * 2017-10-16 2018-05-01 山东科技大学 A kind of coal mine roadway anchoring whitewashing microorganism self-healing system and its construction method
CN108558260B (en) * 2018-05-24 2020-12-01 中交四航工程研究院有限公司 Self-healing concrete complex enzyme microcapsule material and preparation method thereof
CN108558260A (en) * 2018-05-24 2018-09-21 中交四航工程研究院有限公司 A kind of self union concrete complex enzyme microencapsulation material and preparation method thereof
CN108947308A (en) * 2018-07-22 2018-12-07 黑龙江工程学院 A kind of concrete external doped dose of permanent seal cooling
CN108947415A (en) * 2018-09-27 2018-12-07 无锡爱路建设有限公司 A kind of preparation method of high-performance road surface repair materials
CN108947415B (en) * 2018-09-27 2021-05-21 无锡爱路科技有限公司 Preparation method of high-performance pavement repair material
CN109231906A (en) * 2018-09-29 2019-01-18 佛山齐安建筑科技有限公司 A kind of preparation method of resistance to compression self-repair concrete
WO2021008341A1 (en) * 2019-07-18 2021-01-21 华南理工大学 Cement-based material crack self-repairing agent capable of curing corrosive sea water ions, and preparation method therefor and application thereof
CN110386794B (en) * 2019-08-28 2020-12-15 罗芳芳 Self-reinforced building concrete and preparation method thereof
CN110386794A (en) * 2019-08-28 2019-10-29 沈华初 A kind of self-reinforcing building concrete and preparation method
CN110776270A (en) * 2019-11-06 2020-02-11 山东大学 Composite structure self-repairing microcapsule particle and preparation method and application thereof
CN111205838A (en) * 2020-02-26 2020-05-29 西南石油大学 Composite microcapsule for fractured rock mass solidification seepage prevention and preparation method thereof
WO2022236998A1 (en) * 2021-05-08 2022-11-17 东南大学 Microbial self-repair agent having viscous product and application thereof
CN114349388A (en) * 2022-01-19 2022-04-15 郑州大学 Water-awakening self-healing microbial capsule and waterproof material prepared from same
CN114349388B (en) * 2022-01-19 2022-11-29 郑州大学 Water-awakening self-healing microbial capsule and waterproof material prepared from same
CN116041019A (en) * 2023-01-17 2023-05-02 中建三局绿色产业投资有限公司 High-crack-resistance corrosion-resistance ultra-high-performance concrete and preparation method thereof

Also Published As

Publication number Publication date
CN108947340A (en) 2018-12-07
CN108947341A (en) 2018-12-07
CN108947340B (en) 2021-10-01
CN108947341B (en) 2021-12-24
CN106746942B (en) 2018-08-28

Similar Documents

Publication Publication Date Title
CN106746942B (en) A kind of metro engineering concrete that can continue selfreparing comprising microbial capsules
CN106045400B (en) Crack autogenous healing concrete using aerobic halophile and preparation method thereof
Tziviloglou et al. Bio-based self-healing concrete: from research to field application
CN103043937B (en) The complex capsule underground structure concrete self-repairing system of a kind of built-in aerobic microorganism
US20210139882A1 (en) Microcapsule of sustainable self-healing coal mine ventilation sealing material crack and preparation method thereof
CN110282903A (en) Microcapsules of repairing concrete crack and preparation method thereof
CN206143071U (en) A little biological glue bag for lasting restore underground works concrete crack
CN104261736A (en) Preparation method of cement-based material having deep self-repairing function
KR102205558B1 (en) Bacterial self-healing coated-carrier for concrete and method thereof
CN105293683B (en) A kind of infiltration type reaction wall composite for PAHs in groundwater pollution amelioration and its preparation method and application method
CN109231906A (en) A kind of preparation method of resistance to compression self-repair concrete
CN105599534A (en) Flaked and powdered mural reinforcing protective agent and preparation and reinforcing method thereof
CN102134480A (en) Water plugging agent and preparation method thereof
CN101570660B (en) Diatom ooze coating special for underground space and preparation and application thereof
CN104163656B (en) A kind of method reinforcing carbonate rock stone cultural artifact
CN111205838A (en) Composite microcapsule for fractured rock mass solidification seepage prevention and preparation method thereof
CN112779182A (en) Portable microorganism-induced calcium carbonate precipitation kit and application thereof in repairing early cracks of pavement base
CN111606596A (en) Concrete anti-cracking and self-repairing composition and preparation and use methods thereof
Reddy et al. Microbial concrete, a wonder metabolic product that remediates the defects in building structures
Ali et al. Microbial calcite induction: a magic that fortifies and heals concrete
Akyel et al. Key applications of biomineralization
CN104773975B (en) A kind of high additive regeneration mineral aggregate/asphalt and preparation method thereof
Wang et al. Research status of self-healing concrete
CN112573941A (en) Method for repairing early cracks of cement stabilized macadam pavement base
Hungria et al. Effect of using magnesium acetate on the self-healing efficiency of hydrogel-encapsulated bacteria in concrete

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
CB03 Change of inventor or designer information

Inventor after: Tang Lubo

Inventor after: Wang Lili

Inventor after: Qin Lei

Inventor after: Zhao Kangkai

Inventor after: Zhang Huawei

Inventor after: Lin Fantong

Inventor after: Li Zhuangzhuang

Inventor after: Sun Mingyue

Inventor after: Wang Pengfei

Inventor before: Tang Lubo

Inventor before: Zhang Huawei

Inventor before: Zhao Kangkai

Inventor before: Lin Fantong

Inventor before: Li Zhuangzhuang

Inventor before: Wang Lili

Inventor before: Wang Pengfei

CB03 Change of inventor or designer information
GR01 Patent grant
GR01 Patent grant
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20180828

Termination date: 20181116

CF01 Termination of patent right due to non-payment of annual fee