CN106728107A - A kind of Chinese medicine composition for improving dizziness - Google Patents
A kind of Chinese medicine composition for improving dizziness Download PDFInfo
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- CN106728107A CN106728107A CN201710003941.4A CN201710003941A CN106728107A CN 106728107 A CN106728107 A CN 106728107A CN 201710003941 A CN201710003941 A CN 201710003941A CN 106728107 A CN106728107 A CN 106728107A
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- halimasch
- dizziness
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/488—Pueraria (kudzu)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
- A61K36/605—Morus (mulberry)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/734—Crataegus (hawthorn)
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention is a kind of Chinese medicine composition for improving dizziness, and it is by halimasch fructification(Armillariella mellea), the root of kudzu vine, mulberry leaf, hawthorn, maltodextrin be primary raw material, through the granule that the operation such as water or ethanol solution extraction, concentration, alcohol precipitation, drying, granulation, packaging is made;It is brown color or brown granular shape composition.Chinese medicine composition of the invention, with the healthcare function for improving dizziness;The present invention uses integration of drinking and medicinal herbs material, have the advantages that to absorb rapidly instead of decoction, sugar-free, edible safety, can long-term taking, features good taste, small volume, be easy to carry, overcome prior art not enough.
Description
Technical field
The present invention relates to a kind of Chinese medicine composition for improving dizziness, belong to medicines and health protection food technology field.
Background technology
In the prior art, dizziness is dizzy and dizzy general name.With dim eyesight, blurring of vision and dim nigrescence as dizzy, swoon
Finger is swooned, and is dizzy to regard thing rotation and can not stand, and both often simultaneously and see, therefore claim dizziness.Interior Ménière in modern medicine, neck
Vertebra disease, high blood pressure, anaemia, ICT etc., clinical manifestation is based on dizziness.
Vertigo typically uses chemical drugs(Such as:Fenazil, diazepam, flunarizine etc.)And Chinese medicine(Such as:XUANYUNNING piece,
Tianma xuanyunning heji etc.)Treatment.There is certain toxic and side effect in chemical drugs, part Chinese medicine there is also chronic malicious epiphenomenon, in
Medicine has become " severely afflicated area " of drug abuse, is not suitable for long-term use.Through retrieval, help to improve the guarantor of dizziness healthcare function
Health food has no report.
The content of the invention
It is an object of the invention to:A kind of Chinese medicine composition for improving dizziness is provided.The present invention uses integration of drinking and medicinal herbs
Material, with replace decoction, rapid absorption, sugar-free, can long-term taking, edible safety, features good taste, small volume, be easy to carry, change
The good Chinese medicine composition of kind dizziness effect.
The technical scheme is that:A kind of Chinese medicine composition for improving dizziness, it is characterised in that by the original of following weight
Material is made:Halimasch fructification 45-50%, root of kudzu vine 15-20%, mulberry leaf 15-20%, hawthorn 15-20%, auxiliary material maltodextrin
3-4%.
It is preferred that, improve the Chinese medicine composition of dizziness, it is characterised in that be made up of the raw material of following weight:Halimasch fructification
48.128%th, the root of kudzu vine 16.043%, mulberry leaf 16.043%, hawthorn 16.043%, auxiliary material maltodextrin 3.743%.
A kind of Chinese medicine composition for improving dizziness, it is characterised in that be made up of the raw material of following weight:Halimasch fructification
Extract 30-35%, kudzu root extract 25-30%, mulberry-leaf extract 10-15%, haw thorn extract 6-8%, maltodextrin
20-25%.
It is preferred that, improve the Chinese medicine composition of dizziness, it is characterised in that be made up of the raw material of following weight:Halimasch fructification
Extract 33.33%, kudzu root extract 26.67%, mulberry-leaf extract 10%, haw thorn extract 6.67%, 23.33% group of maltodextrin
Into.
According to theory of traditional Chinese medical science and Chinese medicinal formulae monarch combination principle, halimasch fructification(Armillariella mellea)It is sweet, mild-natured, return
Liver Channel.Suppressing the hyperactive liver to relieve the wind syndrome, dispelling wind and removing obstruction in the meridians, strengthening the muscles and bones is dizzy for all kinds of dizziness, headache, insomnia, and numb limb is monarch drug in a prescription;Pueraria lobota
Root nature and flavor are sweet, pungent, cool.Returns spleen stomach lung channel.Expelling pathogenic factors from muscles and skin is brought down a fever, and is promoted the production of body fluid, and promoting eruption is clearing and activating the channels and collaterals.For dizziness and headache, hypertension causes
Stiff nape and back be ministerial drug;Mulberry leaf nature and flavor are sweet, hardship, tremble with fear.Return lung, Liver Channel.For dispelling wind and heat from the body, clearing away the lungheat and moisturizing is clear liver and improved vision.With
In anemopyretic cold, lung-heat type cough, dizziness headache, it is adjutant that hot eyes is dim-sighted;Hawthorn nature and flavor are sour, sweet, tepor.Returns spleen, stomach, Liver Channel,
Food digesting stomach fortifying, scattered stasis, for hyperlipidemia, improve microcirculation to make medicine.All medicines share, for helping to improve various dizzy
The symptoms such as dizzy, headache, insomnia, numb limb.
Halimasch Armillaria mellea(Vahl. Ex Fr.)Quel. it is under the jurisdiction of Tricholomataceae
(Tricholomataceae)Armillaria Armillaria(Fr.: Fr.)) Staude., also known as Armillariella mellea, sweet ring gill fungus, Qinggang
Gill fungus, is a kind of dietotherapeutic fungi.
Armillariella mellea is Mycophyta Basidiomycetes Bai Mo sections Armillaria plant halimasch Armillaria mellea(Vahl ex
Fr.)Quel. fructification.Wildness hazel-mushroom is the distinctive edible fungus in Northeast China, is both edible mushroom and medicinal fungi.My god
Fiber crops are similar to the pharmacological action of halimasch, and being embodied in the two has calmness, analgesia, hypotensive, hypoglycemic, antibacterial, improvement
Memory, anti-oxidant, high immunity of organisms, the effect of anti-aging.These similar pharmacological actions all point out with rhizoma Gastrodiae and
Polysaccharide constituents in halimasch are relevant.
Armillariella mellea medicinal material record in《National Chinese herbal medicine compilation》, Armillariella mellea nature and flavor are sweet, temperature.Dispelling, collateral-activating, strengthening the muscles and bones.For each
Plant the diseases such as waist-leg pain, rickets, epilepsy.Armillariella mellea medicinal material also record in《Dictionary of medicinal plant》, with dispelling, collateral-activating, strengthen muscles and bones
Function.Control epilepsy, various lumbocrural pains, rickets.
The technical requirements of Armillariella mellea are recorded in professional standard LY/T 2465-2015《Armillariella mellea》, national standard execution GB 7096-
2014《National food safety standard edible mushroom and its product》Standard specifies.
Armillaria mycelium is the nutrition organs of halimasch, is extremely very thin filamentous.In the tree of raw halimasch shoestring
It is subcutaneous, it is seen that the mycelia block or ozonium being made up of a large amount of mycelia, in white powder or milky.
Halimasch can be cultivated using fermented and cultured technology, through the halimasch powder of liquid fermentation and culture record in《Country
The drug standards》Chemicals provincial standard rises to national standard(13), standard number is WS-10001-(HD-1224)-
2002.The halimasch powder of liquid fermentation and culture, with arresting convulsion, the effect for relieving dizziness, high fever, infantile convulsions, epilepsy, etc..For treating various dizziness, neurasthenia, mistake
The diseases such as dormancy, tinnitus, extremity numbness.
The root of kudzu vine is legume pueraria lobata Pueraria lobata(Willd.)The dry root of Ohwi, practises and claims elegant jessamine, is medicine food
Dual-purpose composition, record in《Chinese Pharmacopoeia》2015 editions one.Root of kudzu vine nature and flavor are sweet, pungent, cool.Returns spleen, stomach, lung channel.Moved back with expelling pathogenic factors from muscles and skin
Heat, promotes the production of body fluid to quench thirst, promoting eruption, and Shengyang Zhixie is clearing and activating the channels and collaterals, effect of relieving alcoholism.For fever caused by exogenous pathogens headache, stiff nape and back, mouth
Yearningly, quench one's thirst, measles without adequate eruption, hot dysentery, diarrhea, dizziness and headache, hemiplegia, chest impediment and cardialgia, in wine poison wound.
Mulberry leaf for moraceae plants mulberry Morus alba L. dried leaf, be medicine-food two-purpose composition, record in《Chinese Pharmacopoeia》
2015 editions one.Mulberry leaf nature and flavor are sweet, hardship, tremble with fear.Return lung, Liver Channel.Dispelling wind and heat from the body, clearing away the lungheat and moisturizing is clear liver and improved vision.For wind-heat sense
Emit, lung-heat type cough, dizziness headache, hot eyes is dim-sighted.
Hawthorn is rosaceous plant large-fruited Chinese hawthorn pimiatificia Bge.var.m ajor N.E.Br. or hawthorn
The dry mature fruit of Crataegus pinnatifida Bge., is medicine-food two-purpose composition, record in《Chinese Pharmacopoeia》2015
Version one.Nature and flavor are sour, sweet, tepor.Returns spleen, stomach, Liver Channel.Food digesting stomach fortifying, scattered stasis, change turbid lipid-loweringing.For meat stagnation, stomach
Gastral cavity turgor, diarrhea dysentery abdominal pain, blood stasis closed, postpartum stasis, confidant sting, chest impediment and cardialgia, hernia pain, hyperlipidemia.
Specifically, the preparation method is that being achieved in that:
1. halimasch fruiting body extract(That is the preparation of halimasch Thick many candies):Take halimasch fructification(Armillariella mellea)It is ground into 2mm
The meal of~3mm, solid-liquid ratio is 1:20,100 DEG C of temperature, the refluxing extraction that adds water 2 times is for the first time 3 hours, and second is small for 2
When;Merge extract solution, be concentrated in vacuo into relative density 1.10~1.20(Room temperature)Clear cream;Clear cream measures 95% with 3~4 times(Quality
Fraction)Ethanol precipitation 24 hours, sediment is measured with 4~5 times(Mass fraction)>=80 DEG C of hot water dissolvings, precipitate 24~48 after letting cool
Hour, supernatant is taken, water-insoluble is filtered off, thick paste is condensed into, 60 DEG C of temperature below vacuum drying are ground into fine powder, obtain final product honey
Ring bacterium Thick many candies.
2. the preparation of kudzu root extract:The root of kudzu vine is taken, the meal or particle of 2mm~5mm is ground into, is returned with 30% ethanol solution
Stream is extracted 2 times, 2 hours every time, 10 times of amounts is added for the first time, and second plus 8 times of amounts, merging extract solution reclaim ethanol, and extract solution is dense
It is reduced to relative density 1.10~1.20(Room temperature)Clear cream, let cool, plus ethanol makes alcohol content for 70%, precipitation 12 hours~24 is small
When;Supernatant is taken, ethanol is reclaimed, thick paste is condensed into, 60 DEG C of temperature below vacuum drying are ground into fine powder, obtain final product root of kudzu vine extraction
Thing.
3. the preparation of mulberry-leaf extract:Mulberry leaf are taken, meal is ground into, solid-liquid ratio is 1:10,80 DEG C~90 DEG C of temperature is used
70% ethanol solution refluxing extraction 2 times, respectively 1 hour, merging filtrate, reclaim ethanol, be concentrated in vacuo to relative density 1.10~
1.20(Room temperature);Concentrate adds 3 times of amounts(Mass fraction)95% ethanol, precipitates 24 hours~48 hours;Supernatant is taken, second is reclaimed
Alcohol, is condensed into thick paste, and 60 DEG C of temperature below vacuum drying are ground into fine powder, obtain final product mulberry-leaf extract.
4. the preparation of haw thorn extract:Hawthorn is taken, the meal of 2mm~3mm is ground into, 70% ethanol solution, solid-liquid ratio 1 is used:
10,90 DEG C of refluxing extractions 2 times, every time 1.5 hours;Merge extract solution, reclaim ethanol, extract solution be concentrated into relative density 1.10~
1.20(Room temperature);Concentrate adds 3 times of amounts(Mass fraction)95% ethanol, precipitates 24 hours~48 hours;Supernatant is taken, second is reclaimed
Alcohol, is condensed into thick paste, and 60 DEG C of temperature below vacuum drying are ground into fine powder, obtain final product haw thorn extract.
Healthcare function:Help to improve dizziness.
Suitable population:Various dizziness crowds.It is not suitable for crowd:Children.
Eating method and amount:Daily 2~3 times, every time 1 bag.Specification:3g/ bags.
Advantages of the present invention:1st, the present invention helps to improve the healthcare function of dizziness, uses integration of drinking and medicinal herbs thing
Matter, simple production process, with absorbing rapidly instead of decoction, sugar-free, can long-term taking, edible safety, features good taste, small volume,
The advantages of being easy to carry, overcomes prior art not enough.2nd, composition of the invention is based on selection wind-dispelling Chinese medicine.Halimasch generation
For rhizoma Gastrodiae Inflammation Zhijing, cause for hypertension VBI, Meniere's syndrome, vegetative nerve functional disturbance
Dizziness, headache, extremity numbness;The root of kudzu vine, mulberry leaf diverging wind-heat, the dizziness and headache caused for wind-heat, lung heat, red eye, swell pain, eye
Blurred vision is spent;Hawthorn scattered stasis, change turbid lipid-loweringing, for blood stasis cause have a dizzy spell, astasia.Composition each component is common
Cooperation, is used to improve vertigo symptoms.
Brief description of the drawings
Fig. 1 is the influence for extracting solid-liquid ratio to halimasch Thick many candies yield.
Fig. 2 is influence of the Extracting temperature to halimasch Thick many candies yield.
Fig. 3 is influence of the extraction time to halimasch Thick many candies yield.
Fig. 4 is influence of the concentration of alcohol to total flavonoids from mulberry tree leaf content.
Fig. 5 is influence of the temperature to total flavonoids from mulberry tree leaf content.
Fig. 6 is influence of the solid-liquid ratio to total flavonoids from mulberry tree leaf content.
Fig. 7 is influence of the extraction time to total flavonoids from mulberry tree leaf content.
Fig. 8 is influence of the concentration of alcohol to flavone content of hawthorn.
Fig. 9 is influence of the temperature to flavone content of hawthorn.
Figure 10 is influence of the extraction time to flavone content of hawthorn.
Figure 11 is influence of the solid-liquid ratio to flavone content of hawthorn.
Specific embodiment
Experimental example
The Study on Preparation of Chinese medicine composition of the present invention
1. armillaria mellea Study on Preparation
Armillaria mellea typically uses Hot water extraction, sig water extraction, pickle extraction.But because of diluted acid, diluted alkaline bar
Under part, easily make polysaccharide that the fracture of glycosidic bond occurs, par-tial polysaccharide easily occurs to hydrolyze and reduce the yield of polysaccharide, therefore the present invention
Using Hot water extraction.
The assay of 1.1 halimasch Thick many candies
The assay of halimasch Thick many candies uses SN/T 4260-2015《The measure benzene of Thick many candies in outlet plant derived foods
Phenol-sulfuric acid method》Standard gauge location survey is determined.
The influence of 1.2 Extracting temperatures, solid-liquid ratio, the factor of time three to halimasch Thick many candies yield.
1.2.1 influence of the solid-liquid ratio to halimasch Thick many candies yield is extracted.
Influence to halimasch Thick many candies yield in 5 hours is extracted using different solid-liquid ratios, boiling temperature.Result is shown in Fig. 1.
Experimental result shows, is 1 in solid-liquid ratio:When 20, the yield highest of Armillariella mellea Thick many candies further increases in solid-liquid ratio
In the case of, the yield of Thick many candies is not significantly improved.
1.2.2 influence of the Extracting temperature to halimasch Thick many candies yield
Using different temperature, 1:20 solid-liquid ratio extracts the influence to halimasch Thick many candies yield in 5 hours.Result is shown in Fig. 2.
Result of the test shows, Extracting temperature has on the yield of Thick many candies and significantly affects, 100 DEG C with 85 DEG C of extraction conditions under, slightly
Polysaccharide yield can improve more than 60%.
1.2.3 influence of the extraction time to halimasch Thick many candies yield
Using different time, 1:Influence of 20 solid-liquid ratio, boiling temperature to halimasch Thick many candies yield.Result is shown in Fig. 3.
Result of the test shows that extraction time was optimum condition with 5 hours, more than 5 hours yield of Thick many candies without notable
Improve.
1.2.4 halimasch coarse polysaccharide extractive rate Orthogonal Experiment and Design and interpretation of result
Influence Armillariella mellea polysaccharide yield has three factors, i.e. solid-liquid ratio, Extracting temperature and extraction time, and result of the test is shown in Table 1.
Analysis result shows that influence halimasch Thick many candies yield factor primary and secondary order is:Extracting temperature > solid-liquid ratios > is carried
The time is taken, optimal factor is combined as:B3, A3, C2, i.e., using Extracting temperature:100 DEG C, extraction time:5 hours, solid-liquid ratio:1:
30, but solid-liquid ratio and 1:20 are more or less the same, it is considered to cost-effective, and solid-liquid ratio is finally set to 1:20.
2. kudzu root extract Study on Preparation
According to state food pharmaceuticals administration general bureau《Kudzu root extract technical requirements and explanation of compilation》Specified in technology will
Summation preparation method, through analysis, sums up optimal preparation technology.
The assay of Puerarin in 2.1 kudzu root extracts
Puerarin content is determined and performs GB/T 22251-2008《The measure of Puerarin in health food》The method that standard specifies
Determine.
2.2 Puerarin recovery rate Orthogonal Experiment and Designs and interpretation of result
Respectively with water, 30% ethanol, 50% ethanol, 80% ethanol as Extraction solvent, 90 DEG C~100 DEG C heating and refluxing extractions twice, often
Secondary 2 hours, 10 times of amounts are added for the first time, second plus 8 times of amounts add ethanol alcohol content is carried out alcohol precipitation for 60%, 70%, 80% respectively
Preparation condition, analyze influence to puerarin content, analysis result is shown in Table 2.
Analysis result shows that influence Puerarin yield factor primary and secondary order is:Extraction solvent > alcohol precipitation concentrations, optimal factor
It is combined as:A2, B2, i.e., extracted 2 times using 30% ethanol solution, and 2 hours every time, solid-liquid ratio was first time plus 10 times of amounts, second
Plus 8 times of amounts, alcohol precipitation concentration 70% is the best conditions of preparation pr ocess.
3. mulberry-leaf extract Study on Preparation
Mulberry leaf mainly contain the active ingredients such as general flavone, alkaloid and polysaccharide, and wherein flavonoid content is larger, yellow
Ketone compounds are soluble in acetone, ethanol, methanol ethyl acetate equal solvent.To use ethanol be solvent to the present invention, separately design with
Different Extracting temperature, different concentration of alcohol, different extraction times, it is determined that optimal extraction conditions.
The assay of 3.1 mulberry-leaf extract general flavones
Mulberry-leaf extract general flavone content is pressed《Health food is checked and assessment technique specification(2003 editions)》It is total yellow in health food
The measure of ketone.
The influence of 3.2 concentration of alcohol, Extracting temperature, solid-liquid ratio, extraction time to total flavonoids from mulberry tree leaf content
3.2.1 influence of the concentration of alcohol to total flavonoids from mulberry tree leaf content
Using solid-liquid ratio 1:10,100 DEG C of Extracting temperature, 1 hour extraction time.Result of the test is shown in Fig. 4.
Result of the test shows, use concentration for 70% and 80% ethanol solution refluxing extraction total flavonoids from mulberry tree leaf extraction effect phase
Closely, in order to cost-effective, concentration of alcohol selection 70% is optimal.
3.2.2 influence of the temperature to total flavonoids from mulberry tree leaf content
Using solid-liquid ratio 1:10, concentration of alcohol 70%, 1 hour extraction time.Result of the test is shown in Fig. 5.
Result of the test shows that refluxing extraction effect preferably, is selected in 80 DEG C~100 DEG C of water-bath using 70% ethanol solution
Selecting 80 DEG C and extracting is conducive to cost-effective, is preferred plan.
3.2.3 influence of the solid-liquid ratio to total flavonoids from mulberry tree leaf content
Using 80 DEG C of temperature, concentration of alcohol 70%, 1 hour extraction time.Result of the test is shown in Fig. 6.
Result of the test shows, using 70% ethanol solution under the conditions of 80 DEG C of refluxing extractions, solid-liquid ratio is 1:10、1:20、1:
30 effects preferably, but are more than 1 in solid-liquid ratio:Under conditions of 10, the content of total flavonoids from mulberry tree leaf rises slowly, therefore selection solid-liquid ratio
It is 1:10 is preferred plan.
3.2.4 influence of the extraction time to total flavonoids from mulberry tree leaf content
Using 80 DEG C of temperature, concentration of alcohol 70%, solid-liquid ratio 1:10.Result of the test is shown in Fig. 7.
Result of the test shows, using 70% ethanol solution under the conditions of 80 DEG C of refluxing extractions, solid-liquid ratio is 1:10, during extraction
Between the content of total flavonoids from mulberry tree leaf is had no significant effect, therefore using extracting 2 times, the preparation technology of 1 hour every time.
3.2.4 mulberry-leaf extract Orthogonal Experiment and Design and interpretation of result
Influence mulberry-leaf extract general flavone content has 4 factors, i.e. solvent selection, Extracting temperature, solid-liquid ratio and extraction time, point
Analysis the results are shown in Table 3.
Analysis result shows that influence total flavonoids from mulberry tree leaf content factor primary and secondary order is:Solid-liquid ratio > Extraction solvents > is extracted
Temperature > extraction times, optimal factor is combined as:C3, A2, B3, D1, i.e., using solid-liquid ratio 1:20, concentration of alcohol 70% extracts temperature
Degree:80 DEG C, 2 hours extraction times.Consider solid-liquid ratio 1:20 and 1:10 influence factors are smaller, for cost-effective, final choice
Solid-liquid ratio 1:10, temperature 70 C~80 DEG C, 70% ethanol solution refluxing extraction 2 times, every time 1 hour optimum process condition.
4. haw thorn extract Study on Preparation
Hawthorn mainly contains general flavone composition, and general flavone is soluble in the organic solvents such as acetone, ethanol.The present invention uses ethanol for molten
Agent, separately designs different Extracting temperatures, concentration of alcohol and extraction time, to determine optimal preparation process condition.
The assay of general flavone in 4.1 haw thorn extracts
Haw thorn extract general flavone content is performed《Health food is checked and assessment technique specification(2003 editions)》It is total in health food
The measure of flavones.
The influence of 4.2 concentration of alcohol, Extracting temperature, solid-liquid ratio, extraction time to flavone content of hawthorn
4.2.1 influence of the concentration of alcohol to flavone content of hawthorn
Using solid-liquid ratio 1:20,90 DEG C of Extracting temperature, 1 hour extraction time.Result of the test is shown in Fig. 8.
Result of the test shows that concentration of alcohol is substantially unchanged from 40%~50%, gradually increases from 50%~60%, works as concentration
Extracting flavonoids amount increase ratio is larger when being raised to 70% and reaches peak.But the continuation with concentration of alcohol increases, and flavones is carried
Output declines, and the flavones that 70% ethanol is extracted is most.
4.2.2 influence of the temperature to extracting flavonoids in hawthorn
Using solid-liquid ratio 1:20, concentration of alcohol 70%, 1 hour extraction time.Result of the test is shown in Fig. 9.
Experiment shows, when temperature 50 C~80 DEG C are gradually increasing, extracting flavonoids amount also with rising, this be due to
Flavones solubility in ethanol increases with the rising of temperature.But when temperature is more than 80 DEG C, one is entered with temperature
Step is raised, and extracting flavonoids amount rises less substantially, gradually balances, and extracts flavones, temperature highest in production from hawthorn
It is easy to use 80 DEG C~90 DEG C.
4.2.3 the influence that extraction time extracts to hawthorn fruit total flavone
Using solid-liquid ratio 1:20, concentration of alcohol 70%, 80 DEG C of Extracting temperature.Result of the test is shown in Figure 10.
Result of the test shows, upon extracting between from 1 hour to 2 hours, general flavone extracted amount steeply rose, but when extracting
Time, further increase over time, general flavone extracted amount declined on the contrary to 3 hours general flavone contents without significant change, because
It is optimal that this uses 2~3 hours extraction times.
4.2.4 the influence that solid-liquid ratio is extracted to hawthorn fruit total flavone
Using concentration of alcohol 70%, 80 DEG C of Extracting temperature, 2 hours extraction times.Result of the test is shown in Figure 11.
Result of the test shows that solid-liquid ratio is smaller to flavone content of hawthorn influence factor, when solid-liquid ratio is more than 1:When 20, carry
Taking rate increases slow, therefore it is 1 to use solid-liquid ratio:20 is optimal.
4.2.5 haw thorn extract Orthogonal Experiment and Design and interpretation of result
Influence haw thorn extract general flavone content has 4 factors, i.e. solvent strength, Extracting temperature, solid-liquid ratio and extraction time, point
Analysis the results are shown in Table 4.
Analysis result shows that influence flavone content of hawthorn factor primary and secondary order is:Extracting temperature > solvent strengths > is carried
Time > solid-liquid ratio is taken, optimal factor is combined as:B3, A3, D3, C3, i.e., using Extracting temperature:90 DEG C, concentration of alcohol 80% is carried
Take 3 hours time material, solid-liquid ratio 1:30.Consider that the influence factor between 70% and 80% concentration of alcohol, each solid-liquid ratio is smaller, in order to
Cost-effective, final choice solid-liquid ratio is 1:10,90 DEG C of Extracting temperature, 70% ethanol solution refluxing extraction 2 times, every time 1.5 hours
Optimum process condition.
5. Chinese medicinal composition preparation research
It is prepared by 5.1 Chinese medicinal composition preparations:Armillaria mellea 1000g, kudzu root extract 800g, mulberry leaf are weighed respectively to extract
Thing 300g, haw thorn extract 200g, are well mixed;Separately take 700g maltodextrin 2000mL cold water to stir, heating is made
Paste makees wetting agent as adhesive, then with 85%~95% edible alcohol, wet granular is made with 14~16 mesh nylon mesh, through 50 DEG C
~60 DEG C of dryings are to moisture≤5.0%(Mass ratio), packing, every bag of 3g is made 1000 bags.
5.2 Chinese medicinal composition preparation functional components and assay
5.2.1 Thick many candies:Press《Health food functional component detection method (2002)》Thick many candies assay in health food
Method is checked, and preparation of the invention contains Thick many candies per 100g:12136mg.
5.2.2 Puerarin:By GB/T 22251-2008《The measure of Puerarin in health food》The method of regulation is determined,
Preparation of the invention contains Puerarin per 100g:925.6mg.
The research of functional component of the present invention and technical requirements
1. the determination of functional component
The present invention is with halimasch(Armillariella mellea), the functional food that is made for primary raw material of the root of kudzu vine, mulberry leaf, hawthorn, with contributing to
Improve the healthcare function of dizziness.
The healthcare function that the present invention has according to main compound contained in composition and these compounds, with reference to
Legal existing test stone, with operability.
Present invention determine that functional component be:Thick many candies and Puerarin.
The healthcare function of 1.1 functional components
1.1.1 Thick many candies
Halimasch is foremost medicine-food two-purpose fungi, and as all mushrooms, halimasch is rich in polysaccharide.Polysaccharide is halimasch tool
There is the primary efficacy material of multiple biological activities.Halimasch shoestring and fructification intracellular polyse are made up of glucose and xylose, bacterium
Glucose and the mol ratio of xylose are 1 in rope polysaccharide:14, fruitbody polysaccharide is 1:10.
Armillariella mellea water-soluble polysaccharide is mainly made up of D-Glucose, containing carbohydrate 94.8%, uronic acid 2.3%, protein 0.5%, point
Son amount is 4.6 × 105Da。
Open good grade and 3 new compounds are separated to from halimasch fructification, wherein 2 is phytosterin compound, one
It is C-18 phytosphingosine type ceramides.
Additionally, there is the report that SG and many mucopolymers are isolated from halimasch fructification.But do not have also
Have and rhizoma Gastrodiae primary pharmacological activity composition --- the report of Gastrodin similar substance.
Rhizoma Gastrodiae is similar to the pharmacological action of halimasch, be embodied in the two have calmness, analgesia, hypotensive, it is hypoglycemic,
Antibacterial, improvement memory, anti-oxidant, high immunity of organisms, the effect of anti-aging.These similar pharmacological actions are all pointed out
It is relevant with the polysaccharide constituents in rhizoma Gastrodiae and halimasch.
1.1.2 Puerarin
The root of kudzu vine mainly contains flavonoids and its glycosides compound, triterpenes and steroid compound, Coumarins and Pueraria lobota phenol glycoside
Compound.Principle active component is isoflavonoid and a small amount of Flavonoid substances in the root of kudzu vine, including:Daidzein,
Daidzin, Puerarin, BiochaninA, biochanin, genistein glycosides, awns handle chrysanthemum element, ononin, 3 '-hydroxyl
Puerarin(PG21), Puerarin xyloside(PG22), 3 ' 2 methoxy puerarins(PG23), Daidezin 4 ', the double glucose of 7-
Glucoside, Puerarin apioside, isoliquiritigenin etc. more than 30 plants isoflavones.
In the active ingredient of the root of kudzu vine, of greatest concern is Puerarin, daidzein etc..Puerarin energy coronary artery dilator
Blood vessel, reduction blood fat strengthens myocardial contractive power, reduces blood pressure, and prevents and treats dizzy hypertension, headache, neck pain.Dai has
Anti-acetylcholine is acted on, it is considered to be the active ingredient of one of root of kudzu vine primary efficacy spasmolysis.
68 vertebro-basilar arterial ischemia patients with vertigo are randomly divided into treatment group and control group by Lv Tongzheng, and treatment group adopts
Treated with puerarin injection, control group gives compound Danshen root injection treatment.Compare two groups of clinical efficacies and vertebral-basilar artery
Blood mean flow rate.As a result treatment group's curative effect is better than control group, and its vertebral-basilar artery blood mean flow rate improves degree and is also better than
Control group.Conclusion:Puerarin is a kind of active drug for treating vertebro-basilar arterial ischemia dizziness.
2. technical requirements research of the invention
The present invention is with GB 16740-2014《National food safety standard health food》With《Healthy food products technical requirements are advised
Model》It is foundation, product technology requirement is studied, all technical is determined, as the foundation for producing and check.
2.1 formulas:Halimasch, the root of kudzu vine, mulberry leaf, hawthorn;Auxiliary material is maltodextrin.
2.2 production technologies:Armillaria mellea, kudzu root extract, mulberry-leaf extract, haw thorn extract are taken, is pasted with malt
Essence is auxiliary material, and particle is made after mixing, and packaging is obtained final product.
Note:Method for preparing extractive is shown in annex
2.3 supplementary material quality requirements
2.3.1 halimasch should meet GB 7096《National food safety standard edible mushroom and its product》Or LY/T 2133《It is gloomy
Woods food Armillariella mellea dried product》Regulation.
2.3.2 the root of kudzu vine, mulberry leaf, hawthorn should meet《Chinese Pharmacopoeia》The regulation of 2015 editions one.
2.3.3 maltodextrin should meet GB/T 20884《Maltodextrin》Regulation.
2.4 technical requirements
2.4.1 organoleptic requirements:Organoleptic requirements are shown in Table 5.
2.4.2 physical and chemical index:Physical and chemical index is shown in Table 6.
2.4.3 microorganism is limited the quantity:Microorganism limitation is shown in Table 7.
2.4.4 functional component or significant composition:Functional component or significant composition are shown in Table 8.
2.5 healthcare functions:Help to improve dizziness.
2.6 suitable populations:Various dizziness crowds.
2.7 are not suitable for crowd:Children
2.8 eating methods and amount:Daily 2~3 times, every time 1 bag.
2.9 specifications:3g/ bags
2.10 storages:Normal temperature, to shady and cool drying.
2.11 shelf-lifves:24 months
Annex:Method for preparing extractive
1. prepared by armillaria mellea:Halimasch fructification is taken, cleaning, drying are ground into meal;Solid-liquid ratio is 1:20, temperature
100 DEG C, the refluxing extraction that adds water 2 times is for the first time 3 hours, and second is 2 hours;Merge extract solution, be concentrated in vacuo into relatively close
Degree 1.10~1.20(Room temperature)Clear cream;Clear cream measures 95% with 3~4 times(Mass fraction)Ethanol precipitation 24 hours, sediment uses 4
~5 times of amounts(Mass fraction)>=80 DEG C of hot water dissolvings, precipitate 24~48 hours after letting cool, and take supernatant, filter off water-insoluble, dense
Shorten thick paste into, 60 DEG C of temperature below vacuum drying are ground into fine powder, obtain final product.
2. prepared by kudzu root extract:The root of kudzu vine is taken, meal is ground into, with 30% ethanol solution refluxing extraction 2 times, 2 is small every time
When, 10 times of amounts are added for the first time, second plus 8 times of amounts, merging extract solution reclaim ethanol, and extract solution is concentrated into relative density 1.10
~1.20(Room temperature)Clear cream, let cool, plus ethanol makes alcohol content for 70%, precipitates 12 hours~24 hours;Supernatant is taken, is reclaimed
Ethanol, is condensed into thick paste, and 60 DEG C of temperature below vacuum drying are ground into fine powder, obtain final product.
3. prepared by mulberry-leaf extract:Mulberry leaf are taken, meal is ground into, solid-liquid ratio is 1:10,80 DEG C~90 DEG C of temperature, with 70%
Ethanol solution refluxing extraction 2 times, respectively 1 hour, merging filtrate, reclaim ethanol, be concentrated in vacuo to relative density 1.10~
1.20(Room temperature);Concentrate adds 3 times of amounts(Mass fraction)95% ethanol, precipitates 24 hours~48 hours;Supernatant is taken, second is reclaimed
Alcohol, is condensed into thick paste, and 60 DEG C of temperature below vacuum drying are ground into fine powder, obtain final product.
4. prepared by haw thorn extract:Hawthorn is taken, the meal being ground into uses 70% ethanol solution, solid-liquid ratio 1:10,90 DEG C are returned
Stream is extracted 2 times, every time 1.5 hours;Merge extract solution, reclaim ethanol, extract solution is concentrated into relative density 1.10~1.20(Room
Temperature);Concentrate adds 3 times of amounts(Mass fraction)95% ethanol, precipitates 24 hours~48 hours;Supernatant is taken, ethanol, concentration is reclaimed
Into thick paste, 60 DEG C of temperature below vacuum drying are ground into fine powder, obtain final product.
The safety research of the present composition:
Main being analyzed with scientific literature, safety evaluatio literature search and screening report etc. of safety research of the invention is commented
Valency, to security and suitable population, is not suitable for crowd, eating method and amount, points for attention etc. and carries out comprehensive study.
Lot of experiments all proves that halimasch mycelia and zymotic fluid all have calm, anticonvulsion, treatment cardiovascular and cerebrovascular disease etc.
Effect, and have no toxic side effect.Separately there is literature research to show, halimasch rhizomorph polysaccharides AMP-1 can increase the sugar tolerance of normal mouse
By force, AMP-1, AMP-2 can suppress the rising of alloxan diabetes mouse blood sugar, and AMP-2 can significantly reduce alloxan glycosuria
The blood sugar of sick mouse, AMP-1, AMP-2(Given low is 10gkg-1·d-1)To free of toxic effects, the interior internal organs for examination mouse
Official is normal lossless.After with the continuous ig mouse 4d of halimasch rhizomorph polysaccharides, do not find mouse because of the drug poisoning phenomena of mortality, internal organ
Organ is normal lossless, has no any adverse reaction.
Tested by acute toxicity test in mice, salmonella typhimurium(Salmonella reversion test), Chinese hamster lung is into fiber finer
Born of the same parents(CHL)Chromosomal aberration test and mice bone marrow micronucleus, investigate mulberry leaf Aqueous extracts acute toxicity and
Genetoxic.As a result there is not death in mulberry leaf Aqueous extracts 40g/kg mouse stomaches administration, and weight compares nothing with Normal group
Notable difference(P〉0.05).Through Salmonella reversion test, the experiment of CHL Chromosome aberrations, mouse microkernel test, the knot of mulberry leaf Aqueous extracts
Fruit is feminine gender.Under conclusion this experiment condition, the maximum dosage of mouse stomach administration of mulberry leaf Aqueous extracts is 40g/kg, and not
Show genetoxic.
The acute toxicology and long term toxicity test of Pueraria Flavonid prove, intraperitoneal injection LD50It is 5.97(5.46~6.48)
G/kg, oral gavage LD50It is 10.11(9.41~10.8)g/kg.To rat long term toxicity:It is always yellow to the oral gavage root of kudzu vine of animal
Ketone dosage is 2.50,1.66 and 0.83g/kg, 1 time/d, is used in conjunction 90d;Animal ordinary circumstance is good, except big, middle dosage is to male
The weight of animals and total white blood cells slightly influence outer, to blood platelet, hemochrome, hepatic and renal function and each main organs Histopathology
Have no significant effect.Prolonged application this product may not produce cumulative toxicity.
Research shows, pollutant mutagenicity detection is carried out to leaves of Hawthorn water extract(Ames)Experiment, mouse bone marrow cells are micro-
Nuclear test and mouse sperm distortion test, have no that leaves of Hawthorn water extract has mutagenesis and embryotoxicity and obvious teratogenesis latent
Power.
With fructus crataegi pinnatifidae medicinal extract to mouse stomach 72h, LD50For(44.6±4)g(Based on crude drug).It is medicinal according to document announcement
Hawthorn is to Mouse oral LD50It is 18.5g.Therefore fructus crataegi pinnatifidae is more much smaller than medicinal hawthorn toxicity.Jin Xueping with 5 groups of Malus doumeri Leaf not
Acute toxicity testing is carried out to mouse with extract solution and maximum safe dosis tolerata is tested.Result shows that the concentration of A, C, E, F group is
2.6g/ml does not have found dead mouse, and its maximum safe dosis tolerata multiple is 132, it is believed that safety non-toxic, and the death rate of D groups
It is 80%, its half lethal dose is determined for 2.1g/ml through further experiment, after illustrating to be extracted through ethanol, the life dissolved by sour water
Alkaloids have toxicity;Pan Ying etc. carries out acute toxicity testing with fructus crataegi pinnatifidae general flavone and maximum safe dosis tolerata is tested, as a result
Show fructus crataegi pinnatifidae general flavone maximum tolerated dose for 168g/kg, equivalent to the crude drug oral consumption per day of adult(33.3g·kg-1·d-1)678 times(According to the average content meter of fructus crataegi pinnatifidae general flavone in the fruit Fructus Crataegi of crude drug), it is believed that safe nothing
Poison.
Dose of one-component is halimasch fructification in composition of the invention(Armillariella mellea)9g, root of kudzu vine 3g, mulberry leaf 3g,
Hawthorn 3g, well below《Chinese Pharmacopoeia》2015 editions and the relevant regulations about medicinal material usage amount.
The combination principle that composition of the invention meets Chinese medicine mutual reinforcement between, mutually makes, also complies with drug matching taboo principle.
Function assessment research of the invention
The present invention, as foundation, is counted with raw material healthcare function with reference to healthcare function scientific literature, literature search and screening report etc.
Analysis and evaluation result, function is claimed carries out comprehensive study.
1. combating vertigo effect
Dizziness and headache, nerve are cured mainly with the halimasch syrup that halimasch nutrient solution, sucrose, citric acid, methyl hydroxybenzoate etc. are made
Weak, insomnia, labyrinthine syndrome.5-HMF is one of main active of Armillaria mellea fermented solution, with anti-oxidant, anti-
Myocardial ischemia, improvement learning memory disorder, and to Alzheimer disease(Alzheimer ' s disease, AD)Have good
Improvement result.
Through clinical research, armillaria mellea polysaccharide has good drug effect to vertigo.
The symptoms such as headache, dizziness, Xiang Qiang and the tinnitus that Pueraria Flavonid, soybean unit and Puerarin cause to hypertension have
Obvious curative effect is especially notable to alleviate headache Xiang Qiangwei.5% glucose injection is added using puerarin injection 400mg
Intravenous infusion in 500ml, treats 62 vertebro-basilar arterial ischemia patients with vertigo, with Salviae Miltiorrhiza Composite in Treating 30 by 1 times/day
Example is compared, and two groups are treated 14d.Result display treatment group total effective rate is 87.1 %, and curative effect is better than control group(P <
0.05).Especially in the treatment atherosis complicated hypertension patient of vertebral-basilar artery and musculi colli strain patient, total effective rate
It is 95%, satisfactory effect.Separately there is document to show, suffered from 36 megrim caused by vertebra-fundus artery blood supply insufficiency of puerarin in treating
Person, total effective rate is 88.8%.
2. sedation
Rhizoma Gastrodiae is similar to the pharmacological action of halimasch, and being embodied in the two has calmness, analgesia, hypotensive, hypoglycemic, suppression
Bacterium, improvement memory, anti-oxidant, high immunity of organisms, the effect of anti-aging.These similar pharmacological actions all point out with
Rhizoma Gastrodiae is relevant with the polysaccharide constituents in halimasch.
The root of kudzu vine has papaverine sample spasmolysis to mouse, guinea pig in vitro intestinal tube.Various isoflavones components are probably diastole
The composition of smooth muscle, contractile element is probably choline, acetylcholine and jam because of materials such as R.
3. anticonvulsant action
Research finds that ip in mice halimasch preparation can strengthen yellow Jackets(Subliminal value HD)Effect, significantly extension
The mouse sleep time that yellow Jackets or hexobarbitone sodium cause, and the convulsions that mouse pentylenetetrazole causes can be protected.
Mouse tail vein injection experiment proves, Armillaria mellea fermented solution can protect the convulsions that pentylenetetrazol causes, to reduce Buddhist nun ancient
Dead mouse number that fourth causes, cerebral blood flow (CBF) and coronary blood flow that dog can be increased;Mouse oral Armillaria mellea fermented solution is tried
Verify bright nonhazardous effect.
4. effect is improved the health care of sleep
Institute of Materia Medica,Chinese Academy of Medical Sciences pharmacological room new drug group is reported, halimasch is injected in mouse peritoneal and is fermented for 1977
Liquid can extend the length of one's sleep of mouse, and have the work cooperateed with the barbital sodium of sub-threshold dose as rhizoma Gastrodiae aqua
With.
5. ringing is improved
Puerarin can reduce blood viscosity, improve microcirculation, and with stimulating central nervous system system, improve brain cell metabolism, protect
Shield brain cell ultra microstructure, improves brain tissue hypoxia-bearing capability, promotes the effect of neurological functional recovery.
Puerarin can be obviously improved normal Golden Hamster brain microcirculation, and what topical drops norepinephrine was caused is micro-
Dyshaemia all improves significantly.Pueraria Flavonid acts on more obvious than coronary vasodilator to cerebral vasodilators, can be leniently
Improve Brain circlulation and peripheral circulation, this improvement result but makes ripple by a narrow margin caused by not individual event expansion of blood vessels increases CBF
Raise, amplitude wave reduction high, extraordinary wave tends to normal.
6. regulating blood lipid action
With 100,200 and 400mgkg-1The mulberry-leaf extract of dosage consolidates continuously to total courage of rat oral gavage 30d, rat blood serum
Alcohol, triacylglycerol content are less than model group, and its HDL-C content is higher than model group, it is seen that mulberry-leaf extract
With regulation blood fat function.There is result of study to show, the anticoagulant active composition of mulberry leaf aqueous extract is a kind of polysaccharide component.
Research shows, 3 dosage of hawthorn fruit total flavone(80、40、20g/kg)There is reduction acute blood-stasis model rat whole blood
The function of promoting blood circulation to disperse blood clots of specific viscosity, Plasma Viscosity and packed cell volume.The centering of hawthorn alcohol extracting thing, undercut blood contrast viscosity have aobvious
Write reduction effect.Hawthorn fruit total flavone shows significant Lipid-regulating effect, has to rat aorta function damage caused by hyperlipidemia
There is obvious protective function, its mechanism may be with the rising of NO, Endothelin(ET)Synthesis reduces relevant.Hawthorn is swollen and ache for stasis blocking,
Blood system can be entered, qi and blood, blood stasis-eliminating and stagnation-dissipating can be passed through and be relieved pain.
7. oxygen lack resistant function
Mouse sc halimasch water extracts, can significantly improve mouse hypoxia-bearing capability, extend the mouse diing time that potassium cyanide causes,
The activity high of the mouse that hyoscine causes substantially is reduced, suppresses nerve cell apoptosis, mitigate delayed neuronal death, protected
Shield ischemic rat brain;Anesthetized dog iv armillaria mycelium preparations, can increase the cerebral blood flow (CBF) and CF of dog.
8. anti-aging effects
Zhejiang Medical university clinical pharmacology research institute computer MSR Information system finds that mulberry leaf are by nervous system function, internal system
The regulation of function and immune system function, and Green Tea Extract, the supplement to trace element and the effect such as control realize that it prolongs
The effect of slow aging.
Research finds that halimasch rhizomorph polysaccharides can significantly extend the life-span of Caenorhabditis elegans, in oxidative stress condition
Under, the expression of HSP-16.2 and SOD-3 in Caenorhabditis elegans body is significantly increased, improve distribution of the DAF-16 albumen in nucleus
Rate, illustrates that halimasch rhizomorph polysaccharides have protection body, the effect of anti-aging.
Composition of the invention is based on selection wind-dispelling Chinese medicine.Halimasch replaces rhizoma Gastrodiae Inflammation Zhijing, for hypertension vertebra
Dizziness, headache, extremity numbness that basilar artery insufficiency, Meniere's syndrome, vegetative nerve functional disturbance cause;The root of kudzu vine, mulberry
Leaf dissipates wind-heat, and the dizziness and headache caused for wind-heat, lung heat, red eye, swell pain, eyes are dim-sighted;Hawthorn scattered stasis, the turbid drop of change
Fat, for blood stasis cause have a dizzy spell, astasia.Composition each component cooperates jointly, is used to improve vertigo symptoms.
Survey on Stability Research of the invention
The present invention according to《Health food stability test guideline》Accelerated stability test is carried out to the present composition, is led to
Overstability is tested, and investigate sample sense organ under difficult environmental conditions, chemistry, physics and biology increases its change with the time
Degree and rule, so that judgement sample packaging, storage requirement and the stability in the shelf-life.
1. accelerated stability test
1.1 test specimens:The particle that the present composition is made, lot number:20150801、20150802、20150803
1.2 instrument and equipments are shown in Table 9
1.3 standard items:Puerarin standard items, purity >=95%;Dextran standard, molecular weight MW5 × 105, purity >=97%.
1.4 experimental conditions:Simulation listing health food packaging, is encapsulated, additional paper using polyester, aluminium, polyethylene composite film
The packaged form of box, is placed in 37 ± 2 DEG C of temperature, relative humidity RH75 ± 5%, avoids being stored 3 months under conditions of light direct projection.
High spot reviews sense organ, moisture, functional component, microorganism etc. are susceptible to the index of change with condition of storage and period of storage.
1.5 test methods:The present composition is made three batches of samples by formula and preparation method, using polyester, aluminium, gathers
Ethene compound membrane bag is encapsulated, and sample is placed in 37 ± 2 DEG C of temperature, relative humidity RH75 ± 5%, avoids light by the packaging of additional carton
Placed 3 months under conditions of line direct projection, be measured by sampling respectively at 1st month, the 2nd month, the 3rd the end of month, high spot reviews sense organ,
The indexs such as moisture, functional component content, microorganism, indices compared with 0th month detection data, investigated its stability.
1.6 methods of inspection
1.6.1 organoleptic requirements:The method tasted with twenty-twenty vision and nasil, mouth is checked;
1.6.2 determination of moisture:Perform GB 5009.3-2016《The measure of moisture in national food safety standard food》.
1.6.3 functional component:Thick many candies assay:Perform《Health food functional component detection method(2002 editions)》;
Puerarin content is determined:Perform GB/T 22251-2008《The measure of Puerarin in health food》.
1.6.4 microorganism:Perform GB 4789.2-2010《National food safety standard food microbiological examination bacterium colony
Sum is determined》、GB 4789.3-2010《National food safety standard food microbiological examination enumeration of coliforms》And GB
4789.15-2016《National food safety standard food microbiological examination mould and yeast counts》.
1.7 Accelerated stability tests the results are shown in Table 10, table 11, table 12
1.8 this product storage requirements and the determination of shelf-life
According to《Health food stability test guideline》In " sample of product quality stability is investigated using accelerated test,
According to accelerated test result, the shelf-life is typically set to 2 years " regulation, the storage practice of this product is:Normal temperature, is placed in shady and cool drying
Place preserves;Shelf-life is 24 months.
Embodiment 1
It is prepared by armillaria mellea:Weigh halimasch fructification(Armillariella mellea)9000g, is ground into the meal of 2~3mm, and solid-liquid ratio is
1:20,100 DEG C of temperature, the 90kg refluxing extractions that add water respectively 2 times are for the first time 3 hours, and second is 2 hours;Merge and extract
Liquid, is concentrated in vacuo to obtain clear cream 3410g;Clear cream 13000mL 95%(Mass fraction)Ethanol precipitation 24 hours, is precipitated thing
2135g;With 10000mL80 DEG C of hot water dissolving, precipitated 24 hours after letting cool, take supernatant, filtered, be condensed into thick paste, 60 DEG C of temperature
Degree vacuum drying, is ground into fine powder, and dry extract 1052g is obtained, and dry extract yield is 11.69%, and dry extract Thick many candies content is
335.60mg/g, Armillariella mellea(Dry weight)Soluble Thick many candies yield is 3.9228%.
Embodiment 2
It is prepared by kudzu root extract:The 3000g roots of kudzu vine are weighed, the meal or particle of 2mm~5mm is ground into, 90 DEG C of refluxing extractions 2 times,
2 hours every time, 30% ethanol solution 30kg, second 30% ethanol solution 24kg are added for the first time, merge extract solution, reclaim ethanol,
It is 1.18 to be concentrated into relative density(Room temperature)Clear cream 1860g is obtained, is let cool, plus ethanol 6000mL, precipitate 24 hours;Take supernatant
Liquid, reclaims ethanol, is condensed into thick paste, and 60 DEG C of temperature below vacuum drying are ground into fine powder, dry extract 956g, dry extract is obtained
Yield is 31.87%, and dry extract puerarin content is 112.04mg/g, the root of kudzu vine(Dry weight)Puerarin yield is 3.5703%.
Embodiment 3
It is prepared by mulberry-leaf extract:3000g mulberry leaf are weighed, meal is ground into, every time with 70% ethanol solution 30kg, 90 DEG C of temperature is returned
Stream is extracted 2 times, 1 hour every time, with merging filtrate, reclaims ethanol, is concentrated in vacuo to relative density 1.18(Room temperature), obtain clear
Cream;Concentrate adds 95% ethanol, precipitates 24 hours~48 hours;Supernatant is taken, ethanol is reclaimed, thick paste, less than 60 DEG C temperature is condensed into
Degree vacuum drying, is ground into fine powder, and mulberry leaf dry extract 326g is obtained, and dry extract yield is 10.87%, dry extract general flavone content
It is 32.52mg/g, mulberry leaf(Dry weight)General flavone yield is 0.3534%.
Embodiment 4
It is prepared by haw thorn extract:Hawthorn 3000g is weighed, the meal of 2~3mm is ground into, respectively with 70% ethanol solution 30kg, 90
DEG C refluxing extraction 2 times, every time 1.5 hours;Merge extract solution, reclaim ethanol, extract solution is concentrated into relative density 1.18(Room temperature),
Clear cream 396g is obtained, clear cream adds 95% ethanol 1200g, precipitates 24 hours, takes supernatant, and ethanol is flung in water-bath, is condensed into thick
Cream, 60 DEG C of temperature below vacuum drying, is ground into fine powder, obtains hawthorn dry extract 213g, and dry extract yield is 7.10%, dry extract
General flavone content is 32.46mg/g, hawthorn(Dry weight)General flavone yield is 0.2305%.
Embodiment 5
It is prepared by Chinese medicine composition of the present invention:Armillaria mellea 1000g, kudzu root extract 800g, mulberry-leaf extract are weighed respectively
300g, haw thorn extract 200g, are put into mixer and are well mixed;90% ethanol solution 1000mL is weighed as wetting agent, mixed
Under conjunction machine running status, composition fine powder surface is slowly sprayed at, stirred;Separately take 700g maltodextrins plus 2000mL water is stirred
Mix it is uniform after, heating is made the liquid of transparence, lets cool to 50 DEG C, is slowly put into above-mentioned composition and stirs;Such as humidity
Deficiency, can supplement 90% ethanol solution, until reaching requirement;14 mesh nylon mesh are taken again is made wet granular with granulator, it is dry through 50 DEG C
Dry, pack, every bag of 3g is made 1000 bags.
Embodiment 6
The Chinese medicine composition of embodiment 5 is determined through AAS and HPLC, wherein:
Halimasch Thick many candies:
(1)Sample treatment:
A. sample extraction:Sample 2.0g is weighed, is placed in 100mL volumetric flasks, add water 80mL, in heating 2 hours on boiling water bath,
Benefit adds water to scale after being cooled to room temperature, is filtered after mixing, discards just filtrate, collects remaining filtrate and supplies precipitate polysaccharides.
B. Thick many candies are precipitated:It is accurate to draw(a)The whole just filtrate 5.0mL of item, is placed in 50mL centrifuge tubes, adds anhydrous second
Alcohol 20mL, after mixing 5min, 5min, abandoning supernatant is centrifuged with 3000r/min.Residue uses 80%(Volume fraction)Ethanol solution
Several milliliters of washings, abandon supernatant after centrifugation, operate 3~4 times repeatedly.Residue water dissolves are simultaneously settled to 5.0mL, after mixing, supply
Precipitated glucan.
C. precipitated glucan:It is accurate to draw(b)Item end solution 2.0mL is placed in 20mL centrifuge tubes, adds 100g/L hydrogen-oxygens
Change sodium solution 2.0mL DDTC solution 2.0mL, 2min is boiled in boiling water bath, cool down, 5min is centrifuged with 3000r/min, discard
Clear liquid.Residue is washed with cleaning solution number milliliter, abandoning supernatant after centrifugation, is operated 3 times repeatedly, and residue uses 10%(Volume fraction)
Sulfuric acid solution 2.0mL dissolves and is transferred in 50mL volumetric flasks, is diluted with water to scale, mixes.This solution is that sample test is molten
Liquid.
(2)Specification Curve of Increasing:It is accurate weigh dextran standards use liquid 0,0.10,0.20,0.40,0.60,0.80,
1.00mL(Equivalent to glucan 0,0.01,0.02,0.04,0.06,0.08,0.10mg)It is respectively placed in 25mL colorimetric cylinders, it is accurate
Really mend and be filled with water to 2.0mL, add 50g/L phenol solution 1.0mL, mixed on rotation vortex mixer, be carefully added into the concentrated sulfuric acid
10.0mL, carefully mixes on rotation vortex mixer, to put and boil 2min in boiling water bath, with spectrophotometer in 485nm ripples after cooling
Length sentences blank reagent solution for reference, 1cm cuvette mensuration absorbance values.With glucan concentration as abscissa, absorbance
It is ordinate, draws standard curve.
(3)Sample determination:Accurate pipette samples determine liquid 2.OmL, are placed in 25mL colorimetric cylinders, plus people's 50g/L phenol is molten
Liquid 1.OmL, mixes on rotation vortex mixer, is carefully added into concentrated sulfuric acid 10.OmL and is carefully mixed on rotation vortex mixer, puts boiling water
2min is boiled in bath, room temperature is cooled to, with spectrophotometer at 485nm wavelength, with reagent blank as reference, 1cm cuvettes
Mensuration absorbance value.Beta-dextran content is found from standard curve, Thick many candies content in sample is calculated, while doing sample blank reality
Test.
Puerarin:
(1)Sample treatment:Accurately weigh 0.50g~1.00g samples(It is accurate to 0.001g), plus appropriate water dissolves, and in ultrasound
Ultrasound 20min in ripple washer, 10mL is settled to after being cooled to room temperature with water, is mixed, 4000r/min centrifugations 5min.Take on 1mL
Macroporous adsorption resin chromatography post on clear liquid, with about 40mL water with 1mL/min drip washing impurity, then elutes the root of kudzu vine with 50mL70% methyl alcohol
Element, is placed in eluent boiling water bath evaporation and closely does, and 1mL is dissolved and be settled to 70% methyl alcohol, and height is treated after crossing 0.45 μm of filter membrane
Effect liquid phase chromatogram is used.
(2)Liquid chromatogram reference conditions:Chromatographic column:ODS C18, 250mm × 4.6mm, 5 μm;Mobile phase:The second of methyl alcohol+36%
Acid+water=25+3+72;Flow velocity:0.6mL/min;Detection wavelength:247nm;Sampling volume:10μL.
The Chinese medicine composition active constituent content:
Thick many candies:121.36mg/g
Puerarin:9.26mg/g
Chinese medicine composition to the improvement dizziness function described in embodiment 5 carries out animal model experiment, it was demonstrated that Chinese medicine of the invention
Composition has the effect for improving vertigo.
1 material and instrument
1.1 animals
Kunming mouse 100, male, 20~22g of body weight;Cavy 50, male and female dual-purpose, 230~260g of body weight, by Jilin
University's Experimental Animal Center is provided.
1.2 medicines
The present composition(Halimasch hawthorn root of kudzu vine particle);Rhizoma Gastrodiae cellulose capsule(Kunming Medicine Group Stock Co., Ltd gives birth to
Produce).
1.3 instruments
Threeway labyrinth is used in experiment(Shanghai Hao Dun Trade Co., Ltd.s);Jumping test instrument(Electricity production stimulator connection diving tower in Shanghai changes
Dress);Gyroscope(The type magnetic force heating stirrer repacking of Shanghai Nanhui Telecommunication Apparatus Factory 681).
2 experimental techniques and result
The influence that 2.1 present compositions are reacted cavy toxemic vertigo
Cavy 50 is taken, 5 groups are randomly divided into:Model group(Physiological saline), control group(Rhizoma Gastrodiae cellulose capsule, 1.00gkg-1), this
Inventive composition(1.0、0.5、0.25g/kg)High, medium and low dosage group.It is administered once daily, successive administration 14 days.Last dose
1h is rapidly injected the μ L/ of chloroform 20 only at 4~5mm in cavy external auditory meatus afterwards, behind 20,40,60min examine,
Number of times, head tremor pendulum number of times are trembled as observation index with animal eyeball, each observing time is 1min.Experimental result is shown in Table 13.
Note:Compare with model group, * P<0.05, * * P<0.01
Table 13 understands that 3 dosage groups of the present composition can reduce cavy toxemic vertigo different time head number of oscillations
With nystagmus number of times(P<0.05 or P<0.01).
2.2 present compositions are acted on mouse combating vertigo
2.2.1 maze experiment
Healthy mice 50 is taken, 5 groups are randomly divided into:Normal group(Physiological saline), positive controls(Rhizoma Gastrodiae cellulose capsule,
1.0g/kg), basic, normal, high group of the present composition(1.0、0.5、0.25g/kg), gastric infusion, once a day, successive administration 3
My god.Experiment mice is carried out continuously Reflex of escaping lightening stroke training in diving tower instrument, and continuous training is for 3 times 1 time, and the daily upper and lower noon is each
Training 1 time, to consolidate its reflection, continuous 3d, it is established that stabilization and firm Reflex of escaping lightening stroke.1h gives before the test after 3 days
Medicine, after training 1 time, in addition to normal group, by mouse, 50r/min at the uniform velocity rotates 10min and all standing on gyroscope, immediately by mouse
It is placed in diving tower instrument, turns on the power, record mouse successfully escapes the time shocked by electricity and jump onto used by platform.Experimental result is shown in Table 2.
2.2.2 Jumping test
Mice group, training, administration and the same maze experiment of dizziness method is caused, record mouse jumps onto for the 1st time flat after being shocked by electricity
Platform and the required time is not fallen.Experimental result is shown in Table 14.
2.2.3 food-intake is determined
Experiment terminates rear quantitative feeding, observes the energy of mouse and records the food-intake in each group mouse 3h.Experimental result is shown in
Table 15.
Note:Compare with Normal group, * * P<0.01
As shown in Table 14, when each dosage group of the present composition and positive group can significantly shorten escape of the mouse in labyrinth
Between, it is statistically significant with blank control group comparing difference(P<0.01);Each dosage group of the present composition and positive group
Substantially shorten the time that mouse successfully jumps onto platform, it is statistically significant with blank control group comparing difference(P<0.01), this hair
The effect of bright composition is presented dose dependent.As shown in Table 15, each dosage group of the present composition and positive group can increase
The mobility of dizziness mouse, can to some extent increase mouse food-intake.
Knowable to being tested more than, the present composition can reduce head number of oscillations and the eyeball shake of cavy toxemic vertigo
Quiver number of times, and the present composition can significantly shorten escape time of the mouse in labyrinth, hence it is evident that shortens mouse and jumps onto escaping for platform
Keep away the time, strengthen the mobility of mouse, it was demonstrated that it also has the drug action of affirmative to mouse dizziness model.In a word, the present invention
Composition can strengthen the mobility of dizziness mouse, body is improved the adaptive faculty of environmental change.
Embodiment 7
A kind of Chinese medicine composition for improving dizziness, it is characterised in that be made up of the raw material of following weight:Halimasch fructification
48.128%th, the root of kudzu vine 16.043%, mulberry leaf 16.043%, hawthorn 16.043%, auxiliary material maltodextrin 3.743%.
Embodiment 8
A kind of Chinese medicine composition for improving dizziness, it is characterised in that be made up of the raw material of following weight:Halimasch fructification is extracted
Thing 33.33%, kudzu root extract 26.67%, mulberry-leaf extract 10%, haw thorn extract 6.67%, maltodextrin 23.33% are constituted.
Embodiment 9
A kind of Chinese medicine composition for improving dizziness:Armillaria mellea 1000g, kudzu root extract 800g, mulberry leaf are weighed respectively to carry
Thing 300g, haw thorn extract 200g are taken, is well mixed;Separately take 700g maltodextrin 2000mL cold water to stir, heating system
Into paste as adhesive, then wetting agent is made with 85%~95% edible alcohol, wet granular is made with 14~16 mesh nylon mesh, through 50
DEG C~60 DEG C of dryings to moisture≤5.0%(Mass ratio), packing, every bag of 3g is made 1000 bags.
Embodiment 10
A kind of Chinese medicine composition for improving dizziness:Halimasch fructification(Armillariella mellea)9000g, root of kudzu vine 3000g, mulberry leaf 3000g, hawthorn
3000g, auxiliary material maltodextrin 700g are constituted.Halimasch fructification, the root of kudzu vine, mulberry leaf, hawthorn extract through water extraction or ethanol respectively, dense
Contract, refine, dry, pulverize into fine powder, then particle is mixed and made into maltodextrin, through drying, packaging, obtaining final product.
Embodiment 11
A kind of Chinese medicine composition for improving dizziness:Halimasch fructification(Armillariella mellea)Extract 1000g, kudzu root extract 800g, mulberry
Leaf extract 300g, haw thorn extract 200g, maltodextrin 700g compositions.Blended, granulation, dry, packaging, obtain final product.Every bag
3g, is made 1000 bags.
Claims (4)
1. it is a kind of improve dizziness Chinese medicine composition, it is characterised in that be made up of the raw material of following weight:Halimasch fructification
45-50%, root of kudzu vine 15-20%, mulberry leaf 15-20%, hawthorn 15-20%, auxiliary material maltodextrin 3-4%.
2. according to the Chinese medicine composition of the improvement dizziness described in claim 1, it is characterised in that be made up of the raw material of following weight:
Halimasch fructification 48.128%, the root of kudzu vine 16.043%, mulberry leaf 16.043%, hawthorn 16.043%, auxiliary material maltodextrin 3.743%.
3. it is a kind of improve dizziness Chinese medicine composition, it is characterised in that be made up of the raw material of following weight:Halimasch fructification is carried
Take thing 30-35%, kudzu root extract 25-30%, mulberry-leaf extract 10-15%, haw thorn extract 6-8%, maltodextrin 20-
25%。
4. according to the Chinese medicine composition of the improvement dizziness described in claim 3, it is characterised in that be made up of the raw material of following weight:
Halimasch fruiting body extract 33.33%, kudzu root extract 26.67%, mulberry-leaf extract 10%, haw thorn extract 6.67%, malt
Dextrin 23.33% is constituted.
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN114487196A (en) * | 2022-01-27 | 2022-05-13 | 鲁南厚普制药有限公司 | Method for establishing UPLC fingerprint of Gastrodia elata vertigo treating granule and fingerprint thereof |
Citations (2)
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CN101249158A (en) * | 2008-04-08 | 2008-08-27 | 鞍山制药有限公司 | Traditional Chinese medicine preparation for preventing and treating cardiovascular and cerebrovascular diseases and preparation |
CN105982980A (en) * | 2015-02-12 | 2016-10-05 | 李萍 | Prescription for promoting cerebral blood supply and for improving Meniere's disease |
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2017
- 2017-01-04 CN CN201710003941.4A patent/CN106728107A/en active Pending
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN101249158A (en) * | 2008-04-08 | 2008-08-27 | 鞍山制药有限公司 | Traditional Chinese medicine preparation for preventing and treating cardiovascular and cerebrovascular diseases and preparation |
CN105982980A (en) * | 2015-02-12 | 2016-10-05 | 李萍 | Prescription for promoting cerebral blood supply and for improving Meniere's disease |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN114487196A (en) * | 2022-01-27 | 2022-05-13 | 鲁南厚普制药有限公司 | Method for establishing UPLC fingerprint of Gastrodia elata vertigo treating granule and fingerprint thereof |
CN114487196B (en) * | 2022-01-27 | 2023-11-14 | 鲁南厚普制药有限公司 | Method for establishing HPLC fingerprint of Gastrodia elata dizzy granule and fingerprint thereof |
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