CN106726914A - A kind of preparation method of anti-aging emulsion - Google Patents
A kind of preparation method of anti-aging emulsion Download PDFInfo
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Abstract
A kind of preparation method of anti-aging emulsion, belongs to biomedicine technical field, and the extracted rear homogeneous of tea free, Tea Flower and tea tree benevolence got off by pruning is prepared from.The emulsion has senile-resistant efficacy to skin, can nti-freckle, go wrinkle and whitening and moistening skin.
Description
Technical field
The invention belongs to biomedicine technical field.
Background technology
Aging is a kind of natural law, skin as one of organ the most significant is changed in biological aging processes, mainly
It is presented with cutis laxa, fold, color spot appearance etc..Explain there are different theories on aging, wherein most influential be
Free radical theory, it considers that the inducement of aging is the free radical produced by the related environment of aging, disease and oneself factor.Environment
Pollution, the irradiation of long-time ultraviolet and smoking can accelerate the senescence process of skin, cause skin histology water shortage, cuticula to be hardened,
Epidermis and skin corium is thinning, collagen fibers of skin is reduced, melanin deposition, thus produce wrinkle, skin become to relax, it is unglazed
The problems such as pool, color spot.
The hypodermic injection of D- galactolipins can reduce the related enzyme activity of Green Tea Extract, accelerate skin senescence, belong to aging modeling
Endogenous factor；Ultraviolet irradiation can influence the functions such as the fibroblastic growth of skin corium, metabolism, belong to aging modeling
Extrinsic factor.
About 1,500,000 hectares of the existing tea place of China, in addition to tender leaf does tea, mistake million tons of branches and leaves, flower, seeds of pruning are all as useless
Gurry is thrown away.Tea free is rich in Tea Polyphenols, and the adjustable Free Radical Metabolism of Human Body of inoxidizability of Tea Polyphenols is balanced, anti-aging,
Prevention and treatment angiocardiopathy, tumour etc..Tea Flower fragrance is fresh and sweet, containing rich in protein, polysaccharide, Tea Polyphenols, amino acid,
Vitamin, trace element, superoxide dismutase and catalase, flavones etc., to human body have removing toxic substances, lipid-loweringing, hypoglycemic, resist
The effects such as cancer, nourishing, beauty treatment.Tea seed kernel oil physicochemical property is similar to olive oil, and contained squalene is blood oxygen therapy agent and biology
Antioxidant, enhance immunity, anti-aging, anti-inflammation and sterilization；Each composition comprehensive function above, has moist skin and repairs skin
The effect of wound, can resist uv damage, and repair cell accelerates wound healing or preventing and treating chapped skin, treatment skin disease etc..
If discarded leaf, flower, seed research and development functional food, health medicine and daily chemical products under comprehensive utilization tea bush trimmer, can be abundant
Using Resources of Tea Plant, drive tea tree breed to update improvement, increase the tea place output value and tea grower's income, promote green barren hill suitable for afforestation.
Report is more to the research of tealeaves both at home and abroad, but is used for for oral administration administration and daily drunk；Tea tree benevolence is ground
Study carefully the extraction of the Tea Saponin, tea seed polysaccharide, grease and the furfural that are limited only to it and contain etc., underuse the angle of anti-oxidation efficacy
MF59, Tea Polyphenols etc., more to have no and prepare emulsion with tea tree leaf, Tea Flower, seed kernel oil as raw material and carry out related anti-ageing honest
The report tested.
The content of the invention
It is that raw material prepares anti-aging breast present invention aim at proposing using discarded tea free, Tea Flower and tea tree benevolence
The method of liquid.
The preparation method of anti-aging emulsion of the present invention is comprised the following steps：
1）Crushed after tea free is cleaned, then with 80 DEG C of flooding 1h, supernatant is collected by centrifugation, by tea tree leaf slag with 80 DEG C of water
1h is extracted again, and supernatant is collected by centrifugation；Merge supernatant liquid filtering twice, filtrate is tea tree leaf extract；Tea tree benevolence is crushed
Ground through 1h after mixing with 60 DEG C of water afterwards, then upper strata grease, i.e. tea tree seed kernel oil are obtained through centrifuging and taking；By Tea Flower through air-flow crushing
Machine ultramicro grinding, obtains Tea Flower Ultramicro-powder；
2）Under processing condition, first by tea tree seed kernel oil instillation tea tree leaf extract, Tea Flower Ultramicro-powder is added, continue equal
Matter, obtains anti-aging emulsion.
Product of the present invention is experimentally verified that with function of anti-skin aging, thus be can be used for nti-freckle, removed wrinkle and whitening and moistening skin.
Contain Tea Saponin in tea free, with surface-actives such as emulsification, foaming, experiment determines that it is 80 to extract preference temperature
DEG C, emulsion foam is serious when temperature is too high, is unfavorable for extracting, and is also unfavorable for the stabilization of tea polyphenols composition.
Tea benevolence is higher because of content of starch, and starch expanded by heating when being extracted oil with mechanical expression method, clogged oil line makes oil yield
Reduce；Tea kind oil is extracted with organic solvent, though recovery rate is very high, there is the essences such as follow-up vacuum distillation recovered solvent and deodorization
The safety such as dissolvent residual and environmental issue etc. in operation processed and product；Supercritical CO2Extraction have extraction temperature it is low, time saving,
It is nontoxic, product can be avoided to aoxidize, without chemical agent residue and pollution, need not refine the advantages of, but financial cost Gao Wei
Industrialization popularization.The present invention crushes tea benevolence, plus 60 DEG C of water make cell water suction thermal expansion, then further destroys cell through colloid mill
Wall, then centrifugation upper strata grease and lower floor's starch.Experiment determination puies forward oily preference temperature for 60 DEG C, because of saponin content in tea benevolence
Also higher, emulsion foam is serious when temperature is too high, and it is unfavorable that oil reservoir is separated, and the too high starch paste viscosity increase of temperature also influences oil
Part separates out.
Tea Flower is not suitable for using aqueous solvent extraction because saponin content is very high, need not also be carried with the organic solvent of high cost
Take or supercritical CO2Extraction., by after Tea Flower ultramicro grinding, breaking-wall cell, contained Tea Saponin chance water is instant, plays emulsification for the present invention
Agent effect make tea tree seed kernel oil stabilization be dispersed in tea tree leaf extract；To have emulsion certain sticky for camellia Ultramicro-powder simultaneously
Property, be conducive to the stabilization of emulsion；Emulsion distributes the naturally pleasant delicate fragrance of camellia.
Further, the water of extraction tea free and the mixing quality ratio of tea free are 5: 1 to the present invention every time.Water is to extract tea
In leaf the effects such as Tea Polyphenols composition solvent, extracted twice using the ratio and met functional component recovery rate requirement,
Meet as water phase needed for dispersion tea tree seed kernel oil（Tea tree leaf extract）Amount is required, economical, reasonable.
The mixing quality ratio of the tea tree kind Renhe water is 1: 10, and such ratio fits the mixed liquor denseness after grinding
When separating oil reservoir beneficial to centrifugation.
The step 2）The r/min of homogenizer rotor speed ＞ 3000 during middle homogeneous, continue equal after adding Tea Flower Ultramicro-powder
The time of matter is 5~10min.Emulsifying agent is made because the present invention is not added with any surfactant, be our experiments show that：Prepare the present invention
Anti-aging emulsion needs that under ＞ 3000r/min processing conditions larger shearing force could be produced, and enables the uniform breast of water-oil phase
Change, 5~10min of homogenizing time can form stable emulsion.
In addition, the materials mass ratio of tea free of the present invention, tea tree benevolence and Tea Flower is 10: 10: 1.Tea free is
The raw material of the effects such as extracting Tea Polyphenols composition, previous experiments determine senile-resistant efficacy composition epigallocatechin in tea free
Gallate（EGCG）And epicatechin（EC）Content, on this basis determine tea free consumption；Previous experiments are also determined
The polishing that adds water extracts the content of squalene in the recovery rate and oil of tea tree seed kernel oil, and tea tree benevolence is determined on this basis
Consumption；Find that Tea Flower contains a large amount of saponin, has thickening power concurrently and determines its consumption as emulsifying agent in experiment.
Brief description of the drawings
Fig. 1 is EGCG standard reference material high-efficient liquid phase chromatograms.
Fig. 2 is EC standard reference material high-efficient liquid phase chromatograms.
Fig. 3 is squalene standard reference material high-efficient liquid phase chromatogram.
Fig. 4 is that Detection wavelength is the anti-aging emulsion highly effective liquid chromatogram of 278nm.
Fig. 5 is that Detection wavelength is the anti-aging emulsion highly effective liquid chromatogram of 210nm.
Fig. 6 is basis of microscopic observation normal group mouse skin epidermis form photo.
Fig. 7 is basis of microscopic observation model group mouse skin epidermis pathological change photo.
Fig. 8 is basis of microscopic observation positive controls mouse skin epidermis pathological change photo.
Fig. 9 is basis of microscopic observation anti-aging emulsion low dose group mouse skin epidermis pathological change photo.
Figure 10 is basis of microscopic observation anti-aging emulsion high dose group mouse skin epidermis pathological change photo.
Figure 11 is the photo of collagenous fibres in basis of microscopic observation normal group mouse skin.
Figure 12 is the photo of collagenous fibres in basis of microscopic observation model group mouse skin.
Figure 13 is the photo of collagenous fibres in basis of microscopic observation positive controls mouse skin.
Figure 14 is the photo of collagenous fibres in basis of microscopic observation anti-aging emulsion low dose group mouse skin.
Figure 15 is the photo of collagenous fibres in basis of microscopic observation anti-aging emulsion high dose group mouse skin.
Specific embodiment
First, the preparation technology of anti-aging emulsion：
1st, 10kg tea frees are taken, is blended after cleaning, 1h is stirred frequently in adding the water that 50kg temperature is 80 DEG C, be then centrifuged for collecting
Supernatant.The tea tree leaf slag after centrifugation is extracted into 1h again with 80 DEG C of kg of water 50 again, supernatant is collected by centrifugation again.Merge twice
Supernatant, through filtering, obtains tea tree leaf extract.
2nd, 10kg tea tree benevolences are taken, the h colloid mills of water 1 grinding that 100kg temperature is 60 DEG C is added after crushing, in centrifugation
Layer grease obtains tea tree seed kernel oil.
3rd, 1kg Tea Flowers are obtained into Tea Flower Ultramicro-powder through airslide disintegrating mill ultramicro grinding.
4th, in 3200 rmin-1During tea tree seed kernel oil instilled into tea tree leaf extract under processing condition, then tea is gradually added into
Tree flower Ultramicro-powder, continues 5~10min of homogeneous and obtains anti-aging emulsion.
2nd, assay of the high performance liquid chromatography to functional component in anti-aging emulsion：
Anti-aging emulsion 20mg accurately is weighed, respectively with methanol constant volume to 10ml, after ultrasonic 30min, with 0.45 μm of miillpore filter
Filtering, filtrate is used as the test liquid 1 for detecting EGCG and EC.
Anti-aging emulsion 500mg, plus n-hexane ultrasonic dissolution are accurately weighed in addition, is filtered, volatilize n-hexane, it is fixed with methyl alcohol
10mL volumetric flasks are dissolved in, after ultrasonic 30min, with 0.45 μm of filtering with microporous membrane, filtrate is used as the confession examination for detecting squalene
Liquid 2.
Chromatographic column is C18（4.6 × 250mm, 5 μm）, flow velocity is 1.0ml/min, 25 DEG C of column temperature, detection EGCG and EC be with
Water: methyl alcohol: glacial acetic acid=84: used as mobile phase, Detection wavelength is 278nm to 15: 1 volume ratio mixed liquor；Detection squalene be with
Methyl alcohol: acetonitrile=6: used as mobile phase, Detection wavelength is 210nm to 4 volume ratio mixed liquor.
Precision draws each μ L of test liquid 20, is determined 3 times by above-mentioned chromatographic condition sample introduction, and calculates sample by criterion keying method
In each functional component content.
The equation of linear regression of EGCG is：Y=529808X-320.31, R2=0.9996, have in 0.05mg~0.4mg
Good linear relationship；The equation of linear regression of EC is：Y=2220370X+3.7073, R2=0.9999,0.025mg~
There is good linear relationship in 0.2mg；The equation of linear regression of squalene is：Y=143760X+907.4, R2=0.9992,
There is good linear relationship in 0.1mg~0.6mg.The results are shown in Table 1 and accompanying drawing 1~5（Ordinate represents that signal is strong in each figure
Degree, AU is voltage amplification factor）.
The HPLC of table 1 determines each functional component content results in anti-aging emulsion
Epigallo-catechin gallate (EGCG), epicatechin and squalene content are respectively in determining anti-aging emulsion
18.26%th, 1.53% and 1.02%.
Epigallo-catechin gallate (EGCG) at retention time 24.848 minutes as seen from Figure 1（EGCG）Chromatographic peak.
Epicatechin at retention time 32.724 minutes as seen from Figure 2（EC）Chromatographic peak.
As seen from Figure 3 at retention time 31.594 minutes squalene chromatographic peak.
Chromatographic peak at retention time 24.942 minutes, belongs to epigallocatechin in anti-aging emulsion as seen from Figure 4
Gallate（EGCG）Detached peaks；Synergy epicatechin in anti-aging emulsion at retention time 32.742 minutes
（EC）Detached peaks.
Chromatographic peak at retention time 32 minutes, belongs to the detached peaks of squalene in anti-aging emulsion as seen from Figure 5.
3rd, the Efficacy experiments of anti-aging emulsion：
（One）In body animal experiment method：
1st, animal packet and treatment：
Packet：30 mouse are taken, male and female half and half are randomly divided into 5 groups, respectively：Normal group, model group, vitamin E positive control
Group（200 mg/kg·d）, anti-aging emulsion low dose group（200 mg/kg·d）, anti-aging emulsion high dose group（400 mg/
kg·d）.
Modeling：Mouse back loses hair or feathers about 5cm × 5 cm, model group, positive controls, anti-aging emulsion low dose group and anti-
The daily ml/100 g of 1 %D- galactolipins of nape part hypodermic injection 1 of aging emulsion high dose group mouse, and carry out 30 watts of uviol lamps
Irradiation, irradiation time is 30 min/d, continues 40 d；Normal group mouse injects isometric physiological saline daily, normal illumination,
Duration is with modeling group.
Administration：Since modeling the 11st day, positive controls, anti-aging emulsion low dose group and anti-aging emulsion high dose
Group mouse smears vitamin E and anti-aging emulsion, normal group and the daily back of model group mouse according to setting dosage back daily
Physiological saline is smeared, continuous smearing investigates mouse back change of skin after 30 days.
2nd, Skin observing：
2.1 skins are visually observed：
Mouse back skin surface is visually observed, by observing the glossiness of skin, smoothness, having immaculate, have nonelastic etc.
The preliminary aging situation for judging mouse skin.
The morphological observation of 2.2 skin histologies：
2.2.1 skin biopsy dyeing：
Cm × 2 cm of clip back skin tissues about 2, removes subcutaneous adipose tissue, in BouinShi fixers after depilation treatment
In be fixed for more than 24 h, after dehydration of alcohol, transparent, waxdip, embedding, section, respectively HE dyeing and Masson trichrome stains, contrast
The microstructure of each group skin.
2.2.2 IMAQ with it is quantitative：
Section to every group of HE dyeing, the shooting, collecting under 200 times of mirrors respectively, every group is selected 4 visuals field, observation skin it is micro-
Structure, measures epidermis and skin corium thickness；To every group of section of Masson trichrome stains, shot under 400 times of mirrors, it is same every group
4 visuals field are selected, the metamorphosis of collagenous fibres is observed, thickness, length and the area ratio of collagenous fibres is determined.Gathered
Image is measured with 6.0 pairs of each indexs of image-pro Plus.
3rd, the measure of skin biochemical indicator：
Take mice skin tissue and be prepared into tissue homogenate, according to kit specification is determined, SOD values, TBA methods are surveyed using hydroxylamine assay
Survey MDA values.Take mouse skin and Hyp values are determined using alkali hydrolysis method, the formula provided according to kit specification is counted needed for calculating
According to.
4th, the measure of moisture content of skin：
Depilation back part skin 2cm × 2cm is taken, precision weighs weight in wet base, and precision weighs dry weight after placing the h of baking oven 24.
。
5th, statistical method：
All testing indexs with means standard deviation () represent, using the softwares of SPSS 17.0 to data result at
Reason analysis and compare, multigroup is compared and use one-way analysis of variance,P<When 0.05, there is significant difference；P<When 0.01, there is pole
Significant difference, is respectively provided with statistical significance.
（Two）In body results of animal：
1st, skin is investigated：
Normal group skin smooth, there is color and luster, flexible；Model group pachylosis and there is fold, epidermis is thinning, lacks flexibility；It is right
Than in model group, positive controls, anti-aging emulsion low dose group epidermal fold high substantially tail off, and skin smooth is glossy；Together
When high dose group have a preferable skin elasticity, skin recovers gloss etc. and is substantially better than low dose group without obvious fold.
2nd, the morphological observation of skin histology：
2.1 HE coloration results：
HE dyeing basis of microscopic observation result is shown in accompanying drawing 6~10（Multiplication factor 200, scale is 100 μm）.
The normal group mouse skin epidermis form that the visible basis of microscopic observation of Fig. 6 is arrived：Mouse skin structural integrity, epidermis
Normal with skin corium thickness, hair follicle and sebaceous glands are full, and epidermis and corium boundary are clearer, dye more uniform.
The model group mouse skin epidermis pathological change situation that the visible basis of microscopic observation of Fig. 7 is arrived：Epidermis and corium are all
Thinning, sweat gland and hair follicle quantity are substantially reduced compared with Fig. 6.
The positive controls mouse skin epidermis pathological change situation that the visible basis of microscopic observation of Fig. 8 is arrived：Mouse skin
Relatively regular, skin corium thickness increases, and hair follicle and sweat gland significantly increase compared with Fig. 7.
The anti-aging emulsion low dose group mouse skin epidermis pathological change situation that the visible basis of microscopic observation of Fig. 9 is arrived：
Mouse skin is relatively regular, and skin corium thickness increases, but hair follicle and sweat gland compare not full enough with Fig. 8, Figure 10.
The anti-aging emulsion high dose group mouse skin epidermis pathological change situation that the visible basis of microscopic observation of Figure 10 is arrived：
Compared with Fig. 7, it was observed that mouse skin is regular, skin corium thickness is dramatically increased and demarcate more visible, even dyeing, hair follicle and skin
Adipose gland substantially increases.
Influence of the 2.2 anti-aging emulsions to mouse aging skin thickness：
The measurement result of epidermis and corium shows, in contrast to normal group, obvious thinning, the anti-aging of epidermis and corium of model group
After emulsion administration, the epidermis and corium of each administration group are dramatically increased（P<0.05,P<0.01）, it is shown in Table 2.
Influence of the anti-aging emulsion of table 2 to mouse aging skin thickness（, n=6）
Note：△Compare with normal group,△△ P<0.01；▲Compare with model group,▲ P<0.05,▲▲ P<0.01。
2.3 Masson trichrome stain results：
The dyeing of Masson trichrome stains collagenous fibres is blueness, sees accompanying drawing 11~15（Multiplication factor 400, scale is 20 μm）.
As seen from Figure 11, collagenous fibres thickness is moderate in the mouse skin of normal group, closely in order.
As seen from Figure 12, arrangement of collagen fibers is loose mixed and disorderly unordered in the mouse skin of model group, and collagenous fibres shorten
The few gap of thin and quantity is big.
As seen from Figure 13, relative to model group, the amount of collagenous fibres substantially increases positive controls in mouse skin, and shape
State recovers normal.
From Figure 14, Figure 15, relative to model group, the amount of collagenous fibres is bright in mouse skin for anti-aging emulsion administration group
It is aobvious to increase, and form recovers normal, and anti-aging emulsion high dose group effect is better than low dose group.
Influence of the 2.4 anti-aging emulsions to mouse aging collagenous tissue parameter：
The measurement result of the thickness of collagenous fibres, length and density shows in mouse skin, compared with normal group, the glue of model group
Fibrillation substantially attenuates, and length shortens, and density diminishes.After administration, relative to model group, positive controls and anti-aging emulsion are low
Dosage group mouse skin collagenous fibres ratio increases, but no significant difference（P>0.05）, the other specification of administration group is equal
Dramatically increase（P<0.01）, it is shown in Table 3.
Influence of the anti-aging emulsion of table 3 to mouse aging collagenous tissue parameter（, n=6）
Note：△Compare with normal group,△△ P<0.01；▲Compare with model group,▲ P<0.05,▲▲ P<0.01。
3rd, the measure of skin Biochemical Indexes：
Result of the test shows, compared with blank group, SOD, Hyp content in model group skin are substantially reduced, and MDA contents are then bright
It is aobvious to increase.Compared with model group, SOD, Hyp content of anti-aging emulsion low dose group skin high are dramatically increased, and MDA contents are notable
Decline（P<0.05,P<0.01）, it is shown in Table 4.
The anti-aging emulsion of table 4 is to mouse aging skin SOD, the influence of MDA, Hyp content（, n=6）
Note：△Compare with normal group,△△ P<0.01；▲Compare with model group,▲ P<0.05,▲▲ P<0.01。
4th, influence of the anti-aging emulsion to mouse aging skin moisture content：
Skin water ratio test shows, compared to normal group, the mouse skin moisture content of model group is reduced；Anti-aging emulsion is just
After dosage administration, the moisture content of mouse skin is dramatically increased compared with model group（P<0.05,P<0.01）, it is shown in Table 4.
Influence of the anti-aging emulsion of table 4 to mouse aging skin moisture content（,n=6）
Note：△Compare with normal group,△△ P<0.01；▲Compare with model group,▲ P<0.05,▲▲ P<0.01。
To sum up, the present invention is more accorded with using D- galactolipins joint ultraviolet irradiation, comprehensive simulation endogenous and exogenous aging factor
True aging course is closed, being successfully established mice aging model carries out anti-aging emulsion Antisenility Experiment.
Experimental result shows：It is a crease in the skin of model group mouse, obscure and have pigment in contrast to the mouse skin of normal group
Calmness, epidermis and skin corium are thinning, and collagenous fibres sequence is disorderly and is broken, superoxide dismutase in skin（SOD）, hydroxyl dried meat ammonia
Acid（Hyp）Content is reduced, MDA（MDA）Content increases, and skin moisture content is reduced.By after anti-aging emulsion percutaneous dosing,
The wrinkle of skin shoals, and glossy, skin smooth, and compared to model group, anti-aging emulsion administration group skin holostrome structure is complete
Whole, the epidermis and skin corium of mouse are thickening, and arrangement of collagen fibers is fine and close and orderly, and collagenous fibres ratio also substantially increases, skin
Middle SOD, Hyp content and moisture content are dramatically increased, and MDA contents are substantially reduced.Illustrate product of the present invention-anti-aging emulsion tool
There is function of anti-skin aging.
Claims (5)
1. a kind of preparation method of anti-aging emulsion, it is characterised in that comprise the following steps：
1）Crushed after tea free is cleaned, then with 80 DEG C of flooding 1h, supernatant is collected by centrifugation, by tea tree leaf slag with 80 DEG C of water
1h is extracted again, and supernatant is collected by centrifugation；Merge supernatant liquid filtering twice, filtrate is tea tree leaf extract；Tea tree benevolence is crushed
Ground through 1h after mixing with 60 DEG C of water afterwards, then upper strata grease, i.e. tea tree seed kernel oil are obtained through centrifuging and taking；By Tea Flower through air-flow crushing
Machine ultramicro grinding, obtains Tea Flower Ultramicro-powder；
2）Under processing condition, first by tea tree seed kernel oil instillation tea tree leaf extract, Tea Flower Ultramicro-powder is added, continue equal
Matter, obtains anti-aging emulsion.
2. the preparation method of anti-aging emulsion according to claim 1, it is characterised in that the water and tea of extraction tea free every time
The mixing quality ratio of leaf is 5: 1.
3. the preparation method of anti-aging emulsion according to claim 1, it is characterised in that the mixing of the tea tree kind Renhe water
Mass ratio is 1: 10.
4. according to claim 1 or 2 or 3 anti-aging emulsion preparation method, it is characterised in that the step 2）Middle homogeneous
When the r/min of homogenizer rotor speed ＞ 3000, continuation homogenizing time is 5~10min.
5. the preparation method of anti-aging emulsion according to claim 1, it is characterised in that the tea free, tea tree benevolence and tea
The materials mass ratio for setting flower is 10: 10: 1.
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1349817A (en) * | 2001-11-06 | 2002-05-22 | 广东轻工职业技术学院 | Composite skin care product containing tea component and its making process |
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| CN103479562A (en) * | 2013-09-12 | 2014-01-01 | 严晓燕 | Gingko green tea moisture keeping emulsion |
| CN105411944A (en) * | 2015-12-23 | 2016-03-23 | 华南农业大学 | Application of tea-oil tree flowers in preparation of skin-care product and tea-oil tree flower facial mask paste |
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