CN106720313A - A kind of probiotics modulation milk powder and preparation method thereof - Google Patents

A kind of probiotics modulation milk powder and preparation method thereof Download PDF

Info

Publication number
CN106720313A
CN106720313A CN201611030937.9A CN201611030937A CN106720313A CN 106720313 A CN106720313 A CN 106720313A CN 201611030937 A CN201611030937 A CN 201611030937A CN 106720313 A CN106720313 A CN 106720313A
Authority
CN
China
Prior art keywords
milk powder
probiotics
powder
oryzanol
temperature
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201611030937.9A
Other languages
Chinese (zh)
Inventor
贾宏信
刘振民
徐致远
苏米亚
陈文亮
齐晓彦
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Shanghai Bright Dairy and Food Co Ltd
Bright Dairy and Food Co Ltd
Original Assignee
Shanghai Bright Dairy and Food Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Shanghai Bright Dairy and Food Co Ltd filed Critical Shanghai Bright Dairy and Food Co Ltd
Priority to CN201611030937.9A priority Critical patent/CN106720313A/en
Publication of CN106720313A publication Critical patent/CN106720313A/en
Pending legal-status Critical Current

Links

Classifications

    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/12Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/13Fermented milk preparations; Treatment using microorganisms or enzymes using additives
    • A23C9/1307Milk products or derivatives; Fruit or vegetable juices; Sugars, sugar alcohols, sweeteners; Oligosaccharides; Organic acids or salts thereof or acidifying agents; Flavours, dyes or pigments; Inert or aerosol gases; Carbonation methods
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/12Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/13Fermented milk preparations; Treatment using microorganisms or enzymes using additives
    • A23C9/1315Non-milk proteins or fats; Seeds, pulses, cereals or soja; Fatty acids, phospholipids, mono- or diglycerides or derivatives therefrom; Egg products
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23CDAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
    • A23C9/00Milk preparations; Milk powder or milk powder preparations
    • A23C9/12Fermented milk preparations; Treatment using microorganisms or enzymes
    • A23C9/13Fermented milk preparations; Treatment using microorganisms or enzymes using additives
    • A23C9/1322Inorganic compounds; Minerals, including organic salts thereof, oligo-elements; Amino-acids, peptides, protein-hydrolysates or derivatives; Nucleic acids or derivatives; Yeast extract or autolysate; Vitamins; Antibiotics; Bacteriocins
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/113Acidophilus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/11Lactobacillus
    • A23V2400/175Rhamnosus
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/51Bifidobacterium
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/51Bifidobacterium
    • A23V2400/513Adolescentes
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/51Bifidobacterium
    • A23V2400/515Animalis
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/51Bifidobacterium
    • A23V2400/517Bifidum
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/51Bifidobacterium
    • A23V2400/519Breve
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/51Bifidobacterium
    • A23V2400/529Infantis
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2400/00Lactic or propionic acid bacteria
    • A23V2400/51Bifidobacterium
    • A23V2400/533Longum

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Polymers & Plastics (AREA)
  • Food Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Nutrition Science (AREA)
  • Molecular Biology (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

A kind of probiotics modulation milk powder disclosed by the invention, raw material include newborn source, vegetable oil, lactose, oryzanol, active probiotic.Its preparation method comprises the following steps that be well mixed for raw material and water by (1), obtains mixed liquor A;(2) B B-complex, newborn mineral salt and the mixed liquor A are well mixed, then carry out homogeneous, sterilized, concentration, spray drying is made original washing powder B;The original washing powder B is well mixed with Freeze-dry Powder of Probioctics, packaging is obtained final product.It is an advantage of the current invention that probiotics modulation milk powder sensory evaluation of the invention is excellent, probiotics is well mixed in modulation milk powder, and probiotics survival rate in milk powder is strong, and the oxidation stability of milk powder is high.

Description

A kind of probiotics modulation milk powder and preparation method thereof
Technical field
The invention belongs to milk powder technical field, specially a kind of probiotics modulation milk powder and preparation method thereof.
Background technology
Human body can be gradually decreased with the increase at age, internal beneficial bacterium.In general, internal flora after people in middle age Structure compared with the flora of children and youth, (wrap by beneficial bacterium (Bacillus acidi lactici and Bifidobacterium) negligible amounts, clostruidium Include bacillus aerogenes capsulatus) it is higher, streptococcus and enterobacteria quantity are also higher.The reduction of beneficial bacterium in human body, can lead Cause to be produced in enteron aisle and contain substantial amounts of putrefaction such as amine, phenol and indoles etc., and the synthesis of B family vitamin can also be reduced, and be entered And there are various intestines problems.
Probiotics is that a class is beneficial to the microorganism of health.Research shows that there is probiotics regulating intestinal canal flora to put down The effects such as weighing apparatus, promotion intestinal health, alleviation lactose intolerance, reduce serum cholesterol, enhance immunity.And milk powder is probiotics Good carrier, the low moisture activity of milk powder, oxygen barrier packaging is not only suitable for the normal temperature of probiotics, and long shelf life is preserved.And Rich in protein and fat are colonized also beneficial to protection probiotics by human gastrointestinal tract environment on intestinal mucosa in milk powder. Probiotics colonizes in enteron aisle, with regulating intestinal canal flora, can suppress the propagation of harmful bacteria, reduces generation or the product of harmful substance It is tired.
Prebiotics (such as inulin, stachyose, polyfructosan) is a kind of prebiotics for being conducive to human body intestinal canal healthy, is demonstrate,proved It is bright to help to adjust functions of intestines and stomach, promote B family vitamin synthesis, the generation of enteral corrupt substance is reduced and suppresses, and suppression has The propagation of evil bacterium, promotes the propagation of beneficial bacterium Bacillus acidi lactici and Bifidobacterium, promotes the absorption of mineral matter, promotes defecation etc. to make With.Oryzanol is a kind of natural organic-compound rich in rice bran oil, is made up of ten several sterols ferulic acid esters One compounds of group.Research shows, oryzanol energy reduction blood fat, the absorption of resistance cholesterol, reduce serum cholesterol, prevents lipid Oxidation, prevention of cardiovascular disease etc. are acted on.
Milk powder this inherently matrix with abundant nutrition material, by improving lipid structure, improves albumen composition, adds Plus micronutrient, prebiotics and probiotics, it more nutrition and is beneficial to health.Such as, probiotics and prebiotics With reference to that can play a part of to promote respective function mutually, there is more positive effect for improving intestinal health;Protein groups Into improvement, be relatively beneficial to albumen digestion and absorb etc..
The content of the invention
To solve the above problems, the present invention provides a kind of probiotics modulation milk powder and preparation method thereof, and the purpose of realization exists In, viability problems of the solution probiotics in modulation milk powder, for people provide nutritious, the ox rich in probiotics and oryzanol Dairy products.Probiotics modulation milk powder of the invention is by cow's milk, inulin, WPC, newborn mineral salt, vegetable oil, Gu Wei Element and probiotics etc. organically combine a modulation milk powder of exploitation, and the inulin of milk powder addition and probiotics contribute to enteron aisle to be good for Health, the oryzanol of addition is beneficial to the physical and mental health of human body.
To achieve these goals, technical scheme disclosed by the invention is:A kind of probiotics modulation breast that the present invention is provided Powder, raw material include newborn source 64.22~77.62%, vegetable oil 3.99%~14.89%, lactose 7.68~13.27%, Gu Wei Element 0.00~0.25%, active probiotic, addition of the active probiotic in milk powder is modulated is 1 × 106Cfu/g~1 × 109cfu/g;The consumption in the newborn source is that conversion is correspondence whole-fat milk powder or the consumption of skimmed milk powder;Described percentage is each Constituent mass accounts for the percentage of each raw material components gross mass in the milk powder in addition to water.
Further, raw material also include prebiotics 3.49~6.36%, WPC 3.99~9.98%, newborn ore deposit Thing salt 0.80~2.40%%, B B-complex 0.20~0.4%%, described percentage accounts for the matter of milk powder raw material for each component Amount percentage.
Wherein, described milk powder is art technology term, and whole-fat milk powder, half skimmed milk powder can be divided into according to fat content And skimmed milk powder.In the present invention, described milk powder preferably uses whole-fat milk powder and/or skimmed milk powder, wherein, described is full-cream Milk powder can be substituted with raw milk commonly used in the art, and described skimmed milk powder can be taken off with commonly used in the art Fat cow's milk is substituted, and the conversion ratio of milk powder and cow's milk is converted according to this area routine ratio, generally:Whole-fat milk powder quality=complete Fat milk quality * 0.117/0.97, skimmed milk powder quality=skimmed milk quality * 0.081/0.96.
Wherein, described vegetable oil is vegetable oil commonly used in the art, generally vegetable oil and/or plant fat Powder, preferably rice bran oil.
Wherein, described lactose is this area routine lactose, refers generally to meet《National food safety standard --- lactose》 The spray-dried lactose or Lactis Anhydrous of GB25595-2010 standards.
Wherein, described WPC is the conventional WPC in this area, preferably WPC34, One or more in WPC50, WPC60, WPC75 and WPC80.
Wherein, described newborn mineral salt is newborn mineral salt commonly used in the art.
Wherein, described oryzanol is oryzanol commonly used in the art.
Wherein, the prebiotics is the conventional prebiotics in this area.Prebiotics (Prebiotics) is a kind of meal supplement Agent, the material passes through optionally to stimulate the growth of the bacterium in a kind of or a small number of kind of bacterium colony with activity so as to there is host The influence of benefit, and then improve the intestinal health of host.The prebiotics is preferably inulin;Wherein, the content of the inulin compared with It is goodly 1~8%, is more preferably 3.5~7.5%, it is therefore preferable to 6.5%;Described percentage is described for each component quality is accounted for The percentage of each raw material components gross mass in probiotics modulation milk powder in addition to water.
Wherein, described B B-complex is B B-complex commonly used in the art.Described B B-complex compared with It is goodly that every gram of B B-complex contains:Vitamin A:6000-14500IU;Vitamin D:1300-2460IU;Vitamin E:100- 200mg;Vitamin C:150-500mg;Vitamin B6:4270-6500μg;Folic acid:1100-2400μg.
Wherein, the active probiotic is this area routine probiotics, and the probiotics refers to that can be reached with viable bacteria state Intestines and stomach, are made up of regulating intestinal canal flora, and the single or specified microorganisms of effect good for health is played to human body or animal Mixed type probiotics.The probiotics is preferably selected from bifidobacterium bifidum (Bifidobacterium bifidum), Ying Ershuan Discrimination bacillus (Bifidobacterium infantis), bifidobacterium adolescentis (Bifidobacterium adolescentis), Bifidobacterium breve (Bifidobacterium breve), bifidobacterium lactis (Bifidobacterium lactis), bifid bar long Bacterium (Bifidobacterium longum), animal bifidobacteria (Bifidobacterium animalis), lactobacillus acidophilus One kind in (Lactobacillus acidophilus) and Lactobacillus rhamnosus (Lactobacillus rhamnosus) or It is various, it is therefore preferable to animal bifidobacteria (Bifidobacterium animalis), wherein the preparation method of the probiotics It is this area customary preparation methods, or can be by commercially available acquisition.The addition of the probiotics is preferably 1 × 106Cfu/g~1 × 109Cfu/g, is more preferably 3 × 107Cfu/g~3 × 108Cfu/g, it is therefore preferable to 3 × 107cfu/g。
Wherein, described water be it is described in the composition of raw materials containing oryzanol modulation milk powder surplus, the consumption of described water It is this area conventional amount used, is typically added when the milk powder is prepared and each component phase homogenous quantities that need to be dissolved with meeting it Water.
Wherein, the water content of the described modulation milk powder containing oryzanol is 2.5~4%, is more preferably 3%.The percentage Than the mass percentage content that milk powder is accounted for for each component.
Wherein, described water activity is the concept of this area common aqueous activity, refers to the shape of moisture presence in food The combination degree (free degree) of state, i.e. moisture and food, water activity value is higher, and its combination degree is lower;Water activity value Lower, its combination degree is higher.Water activity numerical value awRepresent, water activity value is relatively wet equal to what is be expressed as a percentage Degree, preferably 0.15~0.25, it is therefore preferable to 0.20.
Present invention also offers a kind of preparation method of the above-mentioned modulation milk powder containing oryzanol, described preparation method is pressed The mode of stating is carried out:
(1) vegetable oil is preheated to 45~60 DEG C, then by formula by the newborn source, WPC, plant Oil, lactose, oryzanol and prebiotics and water are well mixed, and mixing temperature is 45~60 DEG C, obtains mixed liquor A;
(2) B B-complex, newborn mineral salt and the mixed liquor A are well mixed, then carry out homogeneous, it is sterilized, Concentration, spray drying, is made original washing powder B;
(3) the original washing powder B is well mixed with Freeze-dry Powder of Probioctics, packaging is obtained final product.
In step (1), described is mixed into this area routine operation, is carried out typically in batch mixer.In described mixing Before, the oryzanol is preferably first uniformly dispersed in Activities of Some Plants oil, then mixes with the mixed liquor A again.The plant As long as the consumption of oil can be such that the oryzanol disperses in vegetable oil and well mixed, the preferably described total matter of oryzanol 50~100 times of amount.
In step (2), before described mixing, the B B-complex and newborn mineral salt preferably first disperse equal in water It is even, then mix with the mixed liquor A again.As long as the consumption of the water can make the B B-complex and newborn mineral salt in water Disperse and be well mixed, 8~10 times of preferably described B B-complex and newborn mineral salt gross mass.
In step (2), described homogeneous is this area routine operation, is carried out typically in high pressure homogenizer.The homogeneous It is double-stage homogenization, wherein, the pressure of one-level homogeneous is preferably 10~30MPa, and the pressure of double-stage homogenization is preferably 1.0~ 3.0MPa, the temperature of the homogeneous is preferably 55~65 DEG C.
In step (2), described sterilization is this area routine operation, and described sterilization is general sterilized 10 at 85~95 DEG C ~30s, preferably the sterilization 25s at 88 DEG C.
In step (2), described concentration is this area routine operation, preferably quadruple effect concentration technology, the concentration of gained The solid concentration of milk is 40~50%.The percentage is mass percent.
In step (2), the spray drying is the routine operation of this area, preferably pressure spray dryer, the pressure The feeding temperature of power spray drying is preferably 60~75 DEG C, and EAT is preferably 165~190 DEG C, and temperature of outgoing air is preferably 75 ~95 DEG C.
In step (2), preferably also it is dried after described spray drying.Described drying is preferably in fluid bed In carry out.
In step (2), packing processes are preferably also carried out after described drying.The packaging step is routinely adopted by this area With composite plastic container or iron flask packing and storing.Preferably also include vacuumizing packing container in the packaging step or Inert gas is filled with, makes the residual oxygen amount < 2% in packing container, the inert gas is this area conventional inert gas, Preferably nitrogen or carbon dioxide, the percentage are percent by volume.
In step (3), described mixing is typically carried out in dry-mixed device.The temperature of the mixing is preferably 15~30 DEG C, more preferably it is 20~25 DEG C, it is therefore preferable to which 25 DEG C, relative humidity is preferably 35~50%, is more preferably 35%~40%, Preferably 40%.The percentage is pressure percentage.
In step (3), the method for the mixing is preferably two-stage or three-level dilution method, i.e., first freeze active probiotic Dry powder is premixed with material 1: 10, and the ratio is mass ratio, then carries out three-level dry-mixed.
On the basis of common sense in the field is met, above-mentioned each optimum condition can be combined, and obtain final product each preferable reality of the present invention Example.
Agents useful for same of the present invention and raw material are commercially available.
Positive effect of the invention is:
Probiotics modulation milk powder sensory evaluation of the invention is excellent, and probiotics is well mixed in modulation milk powder, and probiotics exists Survival rate is strong in milk powder, and the oxidation stability of milk powder is high.Probiotics of the present invention modulates milk powder by high temperature Acceleration study After storing for a long time, probiotics is stablized in milk powder with the extension of storage time, prebiotic by after the Acceleration study of 12 weeks The change of the countless magnitudes of viable count of the bacterium in milk powder.Probiotics of the invention modulates milk powder by cow's milk fats portion vegetable oil Replace, and reinforcing prebiotics, vitamin and oryzanol etc., it is beneficial to human body intestinal canal health and physical and mental health.
Specific embodiment
The present invention is described in further detail below by specific embodiment.
Embodiment one:A kind of probiotics modulation milk powder disclosed by the invention, raw material include newborn source 77.62%, vegetable oil 5.20%, lactose 7.68%, prebiotics 4.50%, WPC 3.99%, newborn mineral salt 0.80%, B B-complex 0.2%, the water content is 2.5%, active probiotic, addition of the active probiotic in milk powder is modulated be 1 × 108cfu/g;The consumption in the newborn source is that conversion is correspondence whole-fat milk powder or the consumption of skimmed milk powder;Described percentage is each Constituent mass accounts for the percentage of each raw material components gross mass in the milk powder in addition to water.
Described newborn source is whole-fat milk powder, and the vegetable oil is vegetable oil, and described lactose is spray-dried lactose, described Oryzanol is oryzanol liquid, and described WPC is WPC34;
And/or, the prebiotics is the prebiotics powder of spray drying.
The vegetable oil is preheated to 45~50 DEG C by the method for preparing the milk powder, (1), then by formula by the breast Source, WPC, vegetable oil, lactose, oryzanol and prebiotics and water are well mixed, and mixing temperature is 45~50 DEG C, is obtained To mixed liquor A;
(2) B B-complex, newborn mineral salt and the mixed liquor A are well mixed, then carry out homogeneous, it is sterilized, Concentration, spray drying, is made original washing powder B;
(3) the original washing powder B is well mixed with Freeze-dry Powder of Probioctics, packaging is obtained final product.
In step (1), described water is deionized water;
And/or, described mixing is typically carried out in batch mixer;
And/or, the oryzanol is first uniformly dispersed in vegetable oil.
In step (2), first it is uniformly dispersed in water before the B B-complex and newborn mineral salt mixing.The consumption of the water It is the B B-complex and 8~10 times of newborn mineral salt gross mass;
And/or, described homogeneous is carried out in high pressure homogenizer.The homogeneous is double-stage homogenization, wherein, one-level homogeneous Pressure is 10~25MPa, and the pressure of double-stage homogenization is 1.0~2.5MPa, and the homogenizing temperature is 55~65 DEG C;
And/or, the sterilization temperature is 85~90 DEG C, and the sterilizing time is 10~30s;
And/or, the temperature of described concentration is 40~50 DEG C, and the terminal of the concentration is 40 to be concentrated into solid content ~50%;The percentage is mass percent;
And/or, the spray drying is pressure spray dryer, and the feeding temperature is 60~75 DEG C, the EAT It it is 165~190 DEG C, the temperature of outgoing air is 75~95 DEG C.
In step (3), described being blended in dry-mixed device is carried out, and the temperature of the mixing is 15~30 DEG C;
And/or, the mixed method is two-stage or three-level dilution method.
The experimental technique of survival rate of the probiotics of Different adding amount in milk powder is:
(1) rice bran oil is preheated to 50 DEG C, with rice bran oil dispersed oryzanol, is then mixed with the cow's milk for being preheated to 50 DEG C, And be well mixed WPC, inulin and lactose by formula, mixing temperature is 50 DEG C, obtains mixed liquor A;
(2) 45 DEG C of moisture are preheated to 120kg and dissipate B B-complex and newborn mineral salt, be well mixed with mixed liquor A, then Homogeneous is carried out, sterilized, concentration, spray drying obtains final product original washing powder B;
(3) the original washing powder B is well mixed with Freeze-dry Powder of Probioctics, packaging obtains final product probiotics modulation milk powder.Probiotics is frozen First taken out from raw material storage before dry powder blend, be placed on the surge chamber in dry-mixed workshop consistent with dry-mixed room temperature with equilibrium temperature. Then the modulation milk powder containing varying number probiotics is obtained according to different additions, the quantity according to addition probiotics is 3.0×106CFU/g, 3.0 × 107CFU/g, 3.0 × 108CFU/g.The temperature of the mixing is 25 DEG C, and relative humidity is 50%. The milk powder water activity of the probiotics Different adding amount is 0.18.
(4) after the completion of probiotics is dry-mixed, mixture homogeneity measure is carried out.The index of the uniformity is the work of probiotics in milk powder Bacterium number.
(5) the modulation milk powder of different in moisture activity is placed on 37 DEG C of temperature, the constant temperature of relative humidity 75% is incubated In case, in the 0th, 2,4,6,8,10,12 weeks sampling detection viable counts, the stability of probiotics is verified.
(6) viable count detection method:Take and treat that test sample is positioned over 1~2 hour at room temperature, balance its temperature consistent with room temperature. Weigh 25g powdered milk samples shaken well in 225mL physiological saline.Absorption 1mL is added to 9mL centrifuge tubes, and (concentration is 10-2) 10 times of gradient dilutions are carried out step by step, and the solution 0.2mL for choosing appropriate dilution carries out pouring into experiment.MRS agar mediums are used First 121 DEG C, 15min sterilizings.After pour plate solidification, it is placed in 37 DEG C of anaerobic culture boxes and cultivates 48h.Clump count is chosen 30 Culture dish between~300 is counted.The uniformity of the probiotics of Different adding amount in milk powder the results are shown in Table 1
Uniformity result of the probiotics of table 1 in milk powder
The stability result of probiotics is shown in Table 2 under Different adding amount
The stability of probiotics under the Different adding amount of table 2
Embodiment two:A kind of probiotics modulation milk powder disclosed by the invention, raw material include newborn source 69.70%, vegetable oil 3.99%, lactose 13.27%, oryzanol .250%, prebiotics 3.49%, WPC 9.98%, newborn mineral salt 2.40%, B B-complex 0.40%, the water content is 4%, active probiotic, and the active probiotic is in milk powder is modulated Addition is 1 × 106cfu/g;The consumption in the newborn source is that conversion is correspondence whole-fat milk powder or the consumption of skimmed milk powder;Described Percentage is the percentage that each component quality accounts for each raw material components gross mass in the milk powder in addition to water.
Described newborn source is skimmed milk powder, and the vegetable oil is plant fat powder, and described lactose is Lactis Anhydrous, described Oryzanol is spraying oryzanol powder, and described WPC is WPC50, WPC60, and WPC75, WPC80 are according to arbitrary proportion Mixing;
And/or, the prebiotics is the prebiotics liquid of spray drying.
The vegetable oil is preheated to 50~60 DEG C by the method for preparing the milk powder, (1), then by formula by the breast Source, WPC, vegetable oil, lactose, oryzanol and prebiotics and water are well mixed, and mixing temperature is 50~60 DEG C, is obtained To mixed liquor A;
(2) B B-complex, newborn mineral salt and the mixed liquor A are well mixed, then carry out homogeneous, it is sterilized, Concentration, spray drying, is made original washing powder B;
(3) the original washing powder B is well mixed with Freeze-dry Powder of Probioctics, packaging is obtained final product.
In step (1), described water is deionized water;
And/or, described mixing is typically carried out in batch mixer;
And/or, the oryzanol is first uniformly dispersed in vegetable oil.
In step (2), first it is uniformly dispersed in water before the B B-complex and newborn mineral salt mixing.The consumption of the water It is the B B-complex and 8~10 times of newborn mineral salt gross mass;
And/or, described homogeneous is carried out in high pressure homogenizer.The homogeneous is double-stage homogenization, wherein, one-level homogeneous Pressure is 15~30MPa, and the pressure of double-stage homogenization is 1.5~3.0MPa, and the homogenizing temperature is 55~65 DEG C;
And/or, the sterilization temperature is 85~95 DEG C, and the sterilizing time is 10~30s;
And/or, the temperature of described concentration is 40~50 DEG C, and the terminal of the concentration is 40 to be concentrated into solid content ~50%;The percentage is mass percent;
And/or, the spray drying is pressure spray dryer, and the feeding temperature is 60~75 DEG C, the EAT It it is 165~190 DEG C, the temperature of outgoing air is 75~95 DEG C.
In step (3), described being blended in dry-mixed device is carried out, and the temperature of the mixing is 15~30 DEG C;
And/or, the mixed method is two-stage or three-level dilution method.
Water activity is to the influence experimental technique of probiotics survival rate in milk powder:
(1) rice bran oil is preheated to 550 DEG C, with rice bran oil dispersed oryzanol, is then mixed with the cow's milk for being preheated to 55 DEG C, And be well mixed WPC, inulin and lactose by formula, mixing temperature is 55 DEG C, obtains mixed liquor A;
(2) 45 DEG C of moisture are preheated to 140kg and dissipate B B-complex and newborn mineral salt, be well mixed with mixed liquor A, then Homogeneous is carried out, sterilized, concentration, spray drying;In the spray-drying process, control powder fluid bed drying time constantly The moisture of powder is controlled, to obtain the original washing powder B of different in moisture activity;The water activity of the original washing powder B of 3 batches of gained (aw) it is respectively 0.16,0.19,0.25.
(3) the original washing powder B is well mixed with Freeze-dry Powder of Probioctics, the probiotics that packaging is obtained final product under different in moisture activity is adjusted Milk powder processed.Freeze-dry Powder of Probioctics mixing before first from raw material storage take out, be placed on the surge chamber in dry-mixed workshop with equilibrium temperature with Dry-mixed room temperature is consistent.It is 3.0 × 10 to prepare the probiotics quantity included in gained probiotics modulation milk powder8CFU/g.It is described mixed The temperature of conjunction is 25 DEG C, and relative humidity is 50%.
(4) the modulation milk powder of different in moisture activity is placed on 37 DEG C of temperature, the constant temperature of relative humidity 75% is incubated In case, in the 0th, 2,4,6,8,10,12 weeks sampling detection viable counts, the stability of probiotics is verified.
(5) viable count detection method:Take and treat that test sample is positioned over 1~2 hour at room temperature, balance its temperature consistent with room temperature. Weigh 25g powdered milk samples shaken well in 225mL physiological saline.Absorption 1mL is added to 9mL centrifuge tubes, and (concentration is 10-2) 10 times of gradient dilutions are carried out step by step, and the solution 0.2mL for choosing appropriate dilution carries out pouring into experiment.MRS agar mediums are used First 121 DEG C, 15min sterilizings.After pour plate solidification, it is placed in 37 DEG C of anaerobic culture boxes and cultivates 48h.Clump count is chosen 30 Culture dish between~300 is counted.Probiotic stability the results are shown in Table 3 under different in moisture activity.
The stability of probiotics Bb-12 under the different in moisture activity of table 3
Embodiment three:A kind of probiotics modulation milk powder disclosed by the invention, raw material include newborn source 64.22%, vegetable oil 14.89%, lactose 9.94%, oryzanol 0.20%, prebiotics 6.35%, WPC 2.98%, newborn mineral salt 1.19%, B B-complex 0.25%, the water content is 3%, active probiotic, and the active probiotic is in milk powder is modulated Addition is 1 × 109cfu/g;The consumption in the newborn source is that conversion is correspondence whole-fat milk powder or the consumption of skimmed milk powder;Described Percentage is the percentage that each component quality accounts for each raw material components gross mass in the milk powder in addition to water.
Described newborn source is skimmed milk powder, and the vegetable oil is plant fat powder, and described lactose is Lactis Anhydrous, described Oryzanol is spraying oryzanol powder, and described WPC is WPC50, WPC60, and WPC75, WPC80 are according to arbitrary proportion Mixing;
And/or, the prebiotics is the prebiotics liquid of spray drying.
The vegetable oil is preheated to 45~50 DEG C by the method for preparing the milk powder, (1), then by formula by the breast Source, WPC, vegetable oil, lactose, oryzanol and prebiotics and water are well mixed, and mixing temperature is 45~50 DEG C, is obtained To mixed liquor A;
(2) B B-complex, newborn mineral salt and the mixed liquor A are well mixed, then carry out homogeneous, it is sterilized, Concentration, spray drying, is made original washing powder B;
(3) the original washing powder B is well mixed with Freeze-dry Powder of Probioctics, packaging is obtained final product.
In step (1), described water is deionized water;
And/or, described mixing is typically carried out in batch mixer;
And/or, the oryzanol is first uniformly dispersed in vegetable oil.
In step (2), first it is uniformly dispersed in water before the B B-complex and newborn mineral salt mixing.The consumption of the water It is the B B-complex and 8~10 times of newborn mineral salt gross mass;And/or, described homogeneous is carried out in high pressure homogenizer. The homogeneous is double-stage homogenization, wherein, the pressure of one-level homogeneous is 15~25MPa, the pressure of double-stage homogenization for 1.5~ 2.5MPa, the homogenizing temperature is 55~65 DEG C;And/or, the sterilization temperature be 85~90 DEG C, the sterilizing time be 10~ 30s;And/or, the temperature of described concentration is 40~50 DEG C, the terminal of the concentration for be concentrated into solid content for 40~ 50%;The percentage is mass percent;And/or, the spray drying is pressure spray dryer, and the feeding temperature is 60 ~75 DEG C, the EAT is 165~190 DEG C, and the temperature of outgoing air is 75~95 DEG C.In step (3), it is described be blended in it is dry Carried out in mixed device, the temperature of the mixing is 15~30 DEG C;And/or, the mixed method is two-stage or three-level dilution method.It is prebiotic The oxidation stability of bacterium milk powder and sensory evaluation are:
(1) it is 3.0 × 10 by the probiotics addition in embodiment 17The milk powder of CFU/g, the probiotics in embodiment 2 adds Dosage is 3.0 × 107The milk powder of CFU/g and apply the milk powder of example 3 and be placed on 37 DEG C of temperature, the constant temperature constant temperature training of relative humidity 75% Support in case, the Acceleration study for carrying out shelf life was sampled in the 0th, 4,8,12 weeks.Milk powder is packaged as aluminium foil bag nitrogen-filled packaging, accelerates Condition is 42 DEG C, relative humidity 75%, 12 weeks acceleration time.The index of detection mainly includes aberration (Δ of the milk powder in accelerated period E), the brew of TBARS value (TBA) and milk powder and taste smell.
(2) milk powder brew and taste odor evaluations method are as follows:In the different time points for accelerating, 12 sense organs are asked to be commented respectively Mirror personnel carry out brew to above-mentioned 3 groups of samples and taste smell is evaluated, and evaluation method is blind sample (3 groups of sample random labellings It is a, b, c).Specific evaluation criterion is as shown in table 4.
The evaluation criterion of the sensory evaluation of table 4
The oxidation stability and Analyses Methods for Sensory Evaluation Results of the probiotics milk powder of table 5
From the oxidation stability and Analyses Methods for Sensory Evaluation Results, embodiment 1, embodiment 2 and embodiment of the probiotics milk powder of table 5 3 milk powder shelf stable, 37 DEG C of Acceleration studies 12 weeks, the aberration of milk powder is not reaching to the distinguishable chromatic aberration of naked eyes, breast The degree of oxidation of powder fat increases with the extension degree of oxidation of acceleration time, but combines knowable to the evaluation result for growing smell, The sense organ of milk powder is without obvious difference after accelerating 12 weeks.This explanation milk powder of the invention has stronger stability.
The preferred embodiments of the present invention are the foregoing is only, is not intended to limit the invention, for the skill of this area For art personnel, the present invention can have various modifications and variations.It is all within the spirit and principles in the present invention, made any repair Change, equivalent, improvement etc., should be included within the scope of the present invention.

Claims (9)

1. a kind of probiotics modulates milk powder, it is characterised in that the milk powder raw material include newborn source 64.22~77.62%, vegetable oil 3.99%~14.89%, lactose 7.68~13.27%, oryzanol 0.00~0.25%, active probiotic, the active probiotic Addition in milk powder is modulated is 1 × 106Cfu/g~1 × 109cfu/g;The consumption in the newborn source is for conversion for correspondence is full-cream The consumption of milk powder or skimmed milk powder;Described percentage is that each raw material components that each component quality is accounted in the milk powder in addition to water are total The percentage of quality.
2. probiotics according to claim 1 modulation milk powder, it is characterised in that raw material also include prebiotics 3.49~ 6.36%, WPC 3.99~9.98%, newborn mineral salt 0.80~2.36%, B B-complex 0.20~0.40%, Described percentage accounts for the mass percent of milk powder raw material for each component.
3. probiotics according to claim 1 modulates milk powder, it is characterised in that raw material also include water, the water content It is 2.5-4%, the percentage accounts for the mass percent of raw material for moisture.
4. probiotics according to claim 1 modulation milk powder, it is characterised in that described newborn source be whole-fat milk powder and/or Skimmed milk powder;
And/or, the vegetable oil is vegetable oil and/or plant fat powder;
And/or, described lactose is spray-dried lactose or Lactis Anhydrous;
And/or, the oryzanol is oryzanol liquid or spraying oryzanol powder.
5. probiotics according to claim 2 modulates milk powder, it is characterised in that described WPC is WPC34, WPC50, WPC60, one or more in WPC75, WPC80;
And/or, the prebiotics is the prebiotics powder or prebiotics liquid of spray drying.
6. it is a kind of to prepare the method that probiotics described in claim 1-5 any one modulates milk powder, it is characterised in that the method bag Following steps are included, the vegetable oil is preheated to 45-60 DEG C by (1), then by formula by the newborn source, WPC, plant Thing oil, lactose, oryzanol and prebiotics and water are well mixed, and mixing temperature is 45-60 DEG C, obtains mixed liquor A;
(2) B B-complex, newborn mineral salt and the mixed liquor A are well mixed, then carry out homogeneous, sterilized, concentration, Spray drying, is made original washing powder B;
(3) the original washing powder B is well mixed with Freeze-dry Powder of Probioctics, packaging is obtained final product.
7. the method that probiotics modulates milk powder is prepared according to claim 6, it is characterised in that in step (1), described water It is deionized water;
And/or, described mixing is typically carried out in batch mixer;
And/or, the oryzanol is first uniformly dispersed in vegetable oil.
8. the method that probiotics modulates milk powder is prepared according to claim 6, it is characterised in that in step (2), it is described compound First it is uniformly dispersed in water before vitamin and newborn mineral salt mixing.The consumption of the water is the B B-complex and newborn mineral salt 8~10 times of gross mass;
And/or, described homogeneous is carried out in high pressure homogenizer.The homogeneous is double-stage homogenization, wherein, the pressure of one-level homogeneous It is 01~30MPa, the pressure of double-stage homogenization is 1.0~3.0MPa, and the homogenizing temperature is 55~65 DEG C;
And/or, the sterilization temperature is 85~95 DEG C, and the sterilizing time is 10~30s;
And/or, the temperature of described concentration is 40~50 DEG C, the terminal of the concentration for be concentrated into solid content for 40~ 50%;The percentage is mass percent;
And/or, the spray drying is pressure spray dryer, and the feeding temperature is 60~75 DEG C, and the EAT is 165 ~190 DEG C, the temperature of outgoing air is 75~95 DEG C.
9. the method that probiotics modulates milk powder is prepared according to claim 6, it is characterised in that in step (3), the mixing Carried out in dry-mixed device, the temperature of the mixing is 15~30 DEG C;
And/or, the mixed method is two-stage or three-level dilution method.
CN201611030937.9A 2016-11-17 2016-11-17 A kind of probiotics modulation milk powder and preparation method thereof Pending CN106720313A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201611030937.9A CN106720313A (en) 2016-11-17 2016-11-17 A kind of probiotics modulation milk powder and preparation method thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201611030937.9A CN106720313A (en) 2016-11-17 2016-11-17 A kind of probiotics modulation milk powder and preparation method thereof

Publications (1)

Publication Number Publication Date
CN106720313A true CN106720313A (en) 2017-05-31

Family

ID=58970927

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201611030937.9A Pending CN106720313A (en) 2016-11-17 2016-11-17 A kind of probiotics modulation milk powder and preparation method thereof

Country Status (1)

Country Link
CN (1) CN106720313A (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110754537A (en) * 2019-11-29 2020-02-07 成都桥然生物科技有限公司 Milk powder for preventing children from myopia and preparation method thereof
CN110754538A (en) * 2019-11-29 2020-02-07 成都桥然生物科技有限公司 Milk powder for preventing children from eating food preference and preparation method thereof

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1257707A (en) * 1999-05-31 2000-06-28 宋晓恩 Health-care medicine for improving vitality of old people and its prescription
CN103651857A (en) * 2013-12-27 2014-03-26 光明乳业股份有限公司 Probiotics-containing infant formula, and preparation method and storage method thereof
CN104489102A (en) * 2014-12-26 2015-04-08 光明乳业股份有限公司 Milk powder for middle and old age people and preparation method thereof
CN105494647A (en) * 2015-12-24 2016-04-20 光明乳业股份有限公司 Calcium-fortified milk powder and preparation method thereof

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1257707A (en) * 1999-05-31 2000-06-28 宋晓恩 Health-care medicine for improving vitality of old people and its prescription
CN103651857A (en) * 2013-12-27 2014-03-26 光明乳业股份有限公司 Probiotics-containing infant formula, and preparation method and storage method thereof
CN104489102A (en) * 2014-12-26 2015-04-08 光明乳业股份有限公司 Milk powder for middle and old age people and preparation method thereof
CN105494647A (en) * 2015-12-24 2016-04-20 光明乳业股份有限公司 Calcium-fortified milk powder and preparation method thereof

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110754537A (en) * 2019-11-29 2020-02-07 成都桥然生物科技有限公司 Milk powder for preventing children from myopia and preparation method thereof
CN110754538A (en) * 2019-11-29 2020-02-07 成都桥然生物科技有限公司 Milk powder for preventing children from eating food preference and preparation method thereof

Similar Documents

Publication Publication Date Title
Meybodi et al. Probiotic viability in yoghurt: A review of influential factors
Beheshtipour et al. Supplementation of Spirulina platensis and Chlorella vulgaris algae into probiotic fermented milks
Rashwan et al. Natural nutraceuticals for enhancing yogurt properties: a review
CN104542974B (en) A kind of -3 sour milk powder of omega and its preparation process
CN105724584A (en) Infant formula milk powder with prebiotics and probiotics and method for preparing infant formula milk powder
CN108244252A (en) Probiotics multielement protein powder and preparation method thereof
CN106720347A (en) A kind of neonate's prescription emulsifiable powder containing probiotics and preparation method thereof
CN103651857A (en) Probiotics-containing infant formula, and preparation method and storage method thereof
Chaturvedi et al. The potential of non-dairy synbiotic instant beverage powder: Review on a new generation of healthy ready-to-reconstitute drinks
CN109069555A (en) Towards neonatal for improving the composition of brain function
CN106509130A (en) Hypoallergenic newborn formula milk powder and preparation method thereof
JP2003250528A (en) Survival-improving agent of bacterium belonging to the genus bifidobacterium, proliferation-promoting agent, or method for producing fermentation product containing the bacterium
Dianawati et al. Viability, acid and bile tolerance of spray dried probiotic bacteria and some commercial probiotic supplement products kept at room temperature
CN110447722A (en) A kind of fermented glutinous rice yoghurt premixed powder and preparation method thereof
Irkin et al. Evaluation of cacao-pudding as a probiotic food carrier and sensory acceptability properties
CN108669563A (en) A kind of composition, its application and strengthen immunity, the product for improving allergy
Hati et al. Use of probiotics for nutritional enrichment of dairy products
RU2506801C1 (en) Method for production of cultured milk product with increased iodine content
RU2514579C1 (en) "longevity" bioproduct manufacture method
CN110100896A (en) A kind of yak milk flavor yoghourt and preparation method thereof
CN106720313A (en) A kind of probiotics modulation milk powder and preparation method thereof
Kryuchkova et al. Vegetable ingredients in functional fermented milk products
Shahmoradi et al. Effect of co-encapsulation of Lactobacillus acidophilus LA5 and selenium in hydrogelated matrix of basil seed mucilage/sodium caseinate on properties of set yogurt
CN102960445A (en) Donkey-hide gelatin-red date yoghourt
CN105815449A (en) Nostoc health care yoghourt and preparation method thereof

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
RJ01 Rejection of invention patent application after publication

Application publication date: 20170531

RJ01 Rejection of invention patent application after publication