CN106718900A - It is a kind of that the method for realizing corn tissue culture is matched by light quality - Google Patents
It is a kind of that the method for realizing corn tissue culture is matched by light quality Download PDFInfo
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- CN106718900A CN106718900A CN201611126911.4A CN201611126911A CN106718900A CN 106718900 A CN106718900 A CN 106718900A CN 201611126911 A CN201611126911 A CN 201611126911A CN 106718900 A CN106718900 A CN 106718900A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- Developmental Biology & Embryology (AREA)
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention provides a kind of method for realizing corn tissue culture by light quality proportioning, comprises the following steps：(1) the isolated organ tissue or cell of corn are chosen as explant；(2) step (1) described explant is put into culture matrix, and ensures that explant is in suitable temperature environment；(3) build based on feux rouges, blue light, white light and infrared light and the light quantum ratio of feux rouges, blue light, white light and infrared light is 7:2:1:The compound external implant of light of 1 LED is irradiated；(4) after irradiation certain hour, breaking up again and upgrowth situation for explant is observed.The method for realizing corn tissue culture is matched by light quality described in the invention, using most accurate light quality mix proportion scheme, individually implemented by LED tissue culture lamps, while having saved the energy, meet the process mid-early stage photoinduction of corn tissue culture and later stage photosynthetic light quality demand, and with promoting the function of corn callus fast-growth in atomization again.
Description
Technical field
The invention belongs to field of plant tissue culture technique, and corn is realized more particularly, to a kind of proportioning by light quality
The method of tissue culture.
Background technology
Plant tissue culture is the abbreviation of Plant Tissue Breeding, and Plant Tissue Breeding is one of plant Sterile Culture Methods Used, is root
There is the theory of totipotency according to plant cell, using the in vitro organ of plant such as root, stem, leaf, stem apex, flower, fruit etc.) tissue
(such as forming layer, epidermis, cortex, marrow cell, endosperm) or cell (such as megaspore, sporidiole, body cell) and primary
Plastid, under the conditions of aseptic and suitable synthetic medium and illumination, temperature, pH etc. are artificial, can induce callus, indefinite
Bud, adventitious root, eventually form the subject of whole plant.
From eighties of last century fifties, corn begins to do group training research by scientist, and even to this day, corn tissue culture is always
By domestic and international researcher as the basis for studying other plant tissue culture, also as the best approach of selection high-quality culture medium.
During corn tissue culture, the factor that external environmental condition is induced it and subculture has a significant effect mainly has：Temperature
Degree and illumination；The regulation of temperature height, has not been problem with present technology, and illumination is only the pass for restricting present corn tissue culture
Key.
In the tissue cultures of plant, what illumination influenceed on it is mainly shown as intensity of illumination, light quality, photoperiod.
Intensity of illumination has a major impact to the propagation and Organ Differentiation of cultured cells, and research shows, rational intensity of illumination,
Growth of seedling can be made sturdy；Light confrontation callus induction, the differentiation of the propagation and organ of cultured tissue has a significant effect；Close
The periodicity of illumination of reason is significantly improved for the growth rate of cultured tissue.
Under the conditions of in vitro sterile culture, the effect of illumination be not to provide photosynthesis needed for the energy, its Main Function
It is a kind of induced reaction to organ, to promote formation, the development of root etc. of seedling, only developing into true when seedling
During the seedling of leaf, illumination can just originate as photosynthetic energy.
In traditional plant tissue culture, illumination is provided using fluorescence type fluorescent tube mostly, by the spectrum that it is sent
In comprising 400nm-800nm whole spectrum, the lighting requirements during tissue culture can be met；With LED semiconductor lightings
Continue to develop, fluorescence type fluorescent tube because its energy consumption is big, caloric value is high, short life the shortcomings of gradually taken by LED illumination product
Generation.
At present, LED plant lamps species on the market is a lot, and LED plant tissue culture lamps occupy significant proportion wherein, this
A little LED tissue cultures lamps functionally undoubtedly all lay particular emphasis on the photosynthesis during light impinges upon tissue culture, and ignore light and impinge upon luring in tissue culture
Effect is led, its effect is simultaneously unsatisfactory, having thus resulted in LED plant tissue cultures lamp cannot be completely independent involved in plant tissue culture always
The situation of whole process, is all that LED tissue cultures lamp is used with fluorescence type fluorescent tube collocation in most cases.
The content of the invention
In view of this, the invention is directed to a kind of method for realizing corn tissue culture by light quality proportioning, by LED groups
Training lamp works independently, adjustment LED tissue culture light matter proportioning so that corn tissue culture Growth and Differentiation demand is more pressed close in illumination, is accelerated
Growth of seedling speed, improves seedling percent.
To reach above-mentioned purpose, what the technical scheme of the invention was realized in：
It is a kind of that the method for realizing corn tissue culture is matched by light quality, comprise the following steps：
(1) the isolated organ tissue or cell of corn are chosen as explant；
(2) step (1) described explant is put into culture matrix, and ensures that explant is in suitable temperature environment；
(3) build based on feux rouges, blue light, white light and infrared light and feux rouges, blue light, white light and infrared light actinic light
Quantum density content is 7:2:1:The compound external implant of light of 1 LED is irradiated and observes breaking up again and growing for explant
Situation.
Further, the explant in the step (1) is the maize immature embryos of 15 days after pollination.
Further, total illumination photo flux density of the compound light of LED is 50umol/m in the step (3)2/s。
Further, the time being irradiated to explant in the step (3) is 60 days.
Further, red light wavelength is chosen for 620-630/640-660nm described in the step (3), preferably
657nm。
Further, blue light wavelength is chosen for 440-460nm, preferably 454nm described in the step (3).
Further, white light described in the step (3) chooses the positive white light of 6000K colour temperatures.
Further, infrared light wavelength is chosen for 730-740nm, preferably 735nm described in the step (3).
Relative to prior art, it is following that being matched by light quality described in the invention realizes that the method for corn tissue culture has
Advantage：
(1) method for realizing corn tissue culture is matched by light quality described in the invention, is matched using most accurate light quality
Scheme, is individually implemented by LED tissue culture lamps, while having saved the energy, meet the process mid-early stage photoinduction of corn tissue culture and after
Phase photosynthetic light quality demand, and with promoting the function of corn callus fast-growth in atomization again.
Brief description of the drawings
The accompanying drawing for constituting the part of the invention is used for providing further understanding the invention, present invention wound
The schematic description and description made does not constitute the improper restriction to the invention for explaining the invention.
In accompanying drawing：
Fig. 1 is the bud ratio ratio chart of the lower explant of monochromatic source irradiation described in the invention embodiment；
Fig. 2 is the growth tendency comparison diagram of the lower explant of monochromatic source irradiation described in the invention embodiment；
Fig. 3 is the bud ratio ratio of the lower explant of all kinds of compound proportioning light source irradiation described in the invention embodiment
Figure；
Fig. 4 is the growth tendency contrast of the lower explant of all kinds of compound proportioning light source irradiation described in the invention embodiment
Figure；
Fig. 5 is the blade material accumulation of the lower explant of all kinds of compound proportioning light source irradiation described in the invention embodiment
Amount ratio chart；
Fig. 6 is the coring quantitative proportion of the lower explant of all kinds of compound proportioning light source irradiation described in the invention embodiment
Figure；
Fig. 7 is the coring length ratio of the lower explant of all kinds of compound proportioning light source irradiation described in the invention embodiment
Figure.
Specific embodiment
It should be noted that in the case where not conflicting, the feature in embodiment and embodiment in the invention can
To be mutually combined.
In the description of the invention, it is to be understood that term " " center ", " longitudinal direction ", " transverse direction ", " on ", D score,
The orientation or position relationship of the instruction such as "front", "rear", "left", "right", " vertical ", " level ", " top ", " bottom ", " interior ", " outward " are
Based on orientation shown in the drawings or position relationship, it is for only for ease of description the invention and simplifies description, rather than instruction
Or imply that the device or element of meaning with specific orientation, with specific azimuth configuration and operation, therefore must not be understood that
It is the limitation to the invention.Additionally, term " first ", " second " etc. are only used for describing purpose, and it is not intended that indicating
Or imply relative importance or the implicit quantity for indicating indicated technical characteristic.Thus, " first ", " second " etc. are defined
Feature can express or implicitly include one or more this feature.In the description of the invention, unless separately
It is described, " multiple " is meant that two or more.
, it is necessary to explanation, unless otherwise clearly defined and limited, term " is pacified in the description of the invention
Dress ", " connected ", " connection " should be interpreted broadly, for example, it may be fixedly connected, or be detachably connected, or integratedly
Connection；Can mechanically connect, or electrically connect；Can be joined directly together, it is also possible to be indirectly connected to by intermediary,
Can be two connections of element internal.For the ordinary skill in the art, can be by concrete condition understanding
State concrete meaning of the term in the invention.
Describe the invention in detail below with reference to the accompanying drawings and in conjunction with the embodiments.
Wavelength is chosen for the feux rouges of 657nm, wavelength for the blue light of 454nm, the positive white light of 6000K colour temperatures and wavelength are
The infrared light of 735nm is that light quality matches basis.
If one embodiment of the invention is embodiment one, the embodiment one is comprised the following steps：
(1) the isolated organ tissue or cell of corn are chosen as explant；
(2) step (1) described explant is put into culture matrix, and ensures that explant is in suitable temperature environment；
(3) build based on feux rouges, blue light, white light and infrared light and feux rouges, blue light, white light and infrared light actinic light
Quantum density content is 7:2:1:1 LED light source is irradiated to explant, after irradiating 60 days, observes breaking up again for explant
And upgrowth situation.
Explant in step (1) is the maize immature embryos of 15 days after pollination.
Total illumination photo flux density of the compound light of LED is 50umol/m in step (3)2/s。
The invention uses most accurate light quality mix proportion scheme, is individually implemented by LED tissue culture lamps, has saved the same of the energy
When, meet the process mid-early stage photoinduction of corn tissue culture and later stage photosynthetic light quality demand, and with promoting corn again
The function of callus fast-growth in atomization.
It is R to set feux rouges code name, and blue light code name is B, and white light code name is W, and infrared light code name is FR.
Embodiment two：
LED light source in corn tissue culture method in embodiment one is replaced with into feux rouges, blue light, white light and infrared light respectively
Monochromatic source irradiation explant and carry out tissue cultures respectively 60 days, in the bud ratio such as figure in four under monochromatic source irradiation
Shown in 1, in the growth tendency in four under monochromatic source irradiation as shown in Fig. 2 transverse axis is the time, the longitudinal axis is plant height.
Embodiment three：
LED light source in corn tissue culture method in embodiment one is replaced with into A classes, B classes, C classes, D classes, E classes, F respectively
The compound proportioning light source of class, G classes and H classes, the wherein order of light quality ratio are R：B：W：FR, all kinds of composite light source proportionings are as follows：
Tissue cultures are carried out respectively 60 days to explant under the irradiations of all kinds of compound proportioning light sources, explant sprouts
Rate as shown in figure 3, growth tendency as shown in figure 4, transverse axis be the time, the longitudinal axis is plant height, blade material accumulation such as Fig. 5 institutes
Show, its Leaf material is photopigment, and coring Quantity is as shown in fig. 6, coring length situation is as shown in Figure 7.
Data in embodiment one embody in embodiment three, by the experiment number in comparative example two and embodiment three
According to, in corn tissue culture, combination light quality for promote corn tissue culture development growth effect will far better than monochromatic light quality, wherein
FR and R plays a major role in induction is sprouted, and healing tissue development initial stage effects of the B after plumule is larger, and W is in later stage plant
Had a clear superiority in growing, but plant production process, monochromatic light is all used, plant can be caused normally to produce,
It can be seen that growing process needs polychromatic light mating reaction；It is worth noting that by the light quality after combination, its luminous intensity distribution ratio
Characteristic that occupation rate is shown with monochromatic light is simultaneously differed, and negative effect is had on the contrary；As can be seen that corn tissue culture with
The other plant growth the same requirement for light quality of demand has an optimal critical point, by our proving and comparisoms, in scheme
C light qualities mix proportion scheme either inductivity, callus all more existing spectrum of propagation more effectively, this light quality is matched somebody with somebody
Than also as optimal light quality mix proportion scheme in current corn tissue culture.
The preferred embodiment of the invention is the foregoing is only, creation is not intended to limit the invention, it is all at this
Within the spirit and principle of innovation and creation, any modification, equivalent substitution and improvements made etc. should be included in the invention
Protection domain within.
Claims (7)
- It is 1. a kind of that the method for realizing corn tissue culture is matched by light quality, it is characterised in that：Comprise the following steps：(1) the isolated organ tissue or cell of corn are chosen as explant；(2) step (1) described explant is put into culture matrix, and ensures that explant is in suitable temperature environment；(3) build based on feux rouges, blue light, white light and infrared light and feux rouges, blue light, white light and infrared light photosynthetic light quantum Density content is 7:2:1:The compound external implant of light of 1 LED is irradiated and observes breaking up again and upgrowth situation for explant.
- It is 2. according to claim 1 that the method for realizing corn tissue culture is matched by light quality, it is characterised in that：The step (1) explant in is the maize immature embryos of 15 days after pollination.
- It is 3. according to claim 1 that the method for realizing corn tissue culture is matched by light quality, it is characterised in that：The step (3) total illumination photo flux density of the compound light of LED is 50umol/m in2/s。
- It is 4. according to claim 1 that the method for realizing corn tissue culture is matched by light quality, it is characterised in that：The step (3) red light wavelength described in is chosen for 620-630/640-660nm, preferably 657nm.
- It is 5. according to claim 1 that the method for realizing corn tissue culture is matched by light quality, it is characterised in that：The step (3) blue light wavelength described in is chosen for 440-460nm, preferably 454nm.
- It is 6. according to claim 1 that the method for realizing corn tissue culture is matched by light quality, it is characterised in that：The step (3) white light described in chooses the positive white light of 6000K colour temperatures.
- It is 7. according to claim 1 that the method for realizing corn tissue culture is matched by light quality, it is characterised in that：The step (3) infrared light wavelength described in is chosen for 730-740nm, preferably 735nm.
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| CN201611126911.4A CN106718900A (en) | 2016-12-08 | 2016-12-08 | It is a kind of that the method for realizing corn tissue culture is matched by light quality |
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| CN201611126911.4A CN106718900A (en) | 2016-12-08 | 2016-12-08 | It is a kind of that the method for realizing corn tissue culture is matched by light quality |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107821162A (en) * | 2017-11-07 | 2018-03-23 | 玉溪云星生物科技有限公司 | A kind of large-scale method for producing of babysbreath Plug seedling |
| CN116548204A (en) * | 2023-06-20 | 2023-08-08 | 华南农业大学 | Vegetable seedling light control method suitable for mechanical grafting |
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| CN107821162A (en) * | 2017-11-07 | 2018-03-23 | 玉溪云星生物科技有限公司 | A kind of large-scale method for producing of babysbreath Plug seedling |
| CN116548204A (en) * | 2023-06-20 | 2023-08-08 | 华南农业大学 | Vegetable seedling light control method suitable for mechanical grafting |
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