CN106717294A - The evaluation method of subprostrate sophora seed quality - Google Patents
The evaluation method of subprostrate sophora seed quality Download PDFInfo
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- 238000011156 evaluation Methods 0.000 title claims abstract description 68
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- 238000012360 testing method Methods 0.000 claims abstract description 31
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
- A01C1/025—Testing seeds for determining their viability or germination capacity
Abstract
The invention discloses a kind of evaluation method of subprostrate sophora seed quality, comprise the following steps：Seed lot is divided, and is extracted and is not less than the seed of 1000g and tests sample as sending；Using seed moisture proportion, mass of 1000 kernel, germination percentage and purity as evaluation points；Determine that the weight of moisture content, mass of 1000 kernel, germination percentage and purity is respectively 0.185,0.175,0.425 and 0.215；Determine the evaluation number of evaluation points：Measure the evaluation number of A, B, C, D respectively as seed lot seed moisture proportion, thousand grain weigth, percentage of seedgermination and seed purity；By formula Calculation Estimation desired value：PI=0.185A+0.175B+0.425C+0.215D.The present invention realizes and quantitative assessment is done to the seed quality of subprostrate sophora seed lot, selected evaluation points can effecting reaction seed quality, and easy to operate, reliable results overcome the blindness and hysteresis quality of conventional judgement.
Description
Technical field
The present invention relates to seed quality assessment technique field.It is more particularly related to a kind of subprostrate sophora seed matter
The evaluation method of amount.
Background technology
Subprostrate sophora, also known as root of subprostrate sophora, sophora tonkinensis Gapnep, are the dry root of legume sophora tonkinensis Gapnep, with clearing heat and detoxicating, detumescence profit
Effect of pharynx, pents up for fiery poison, tonsillitis and larynx numbness, abscess of throat, gingivitis, the disease such as aphthae.Subprostrate sophora is a weight
" osmanthus medicine ", " southern medicine " kind wanted, the Chinese patent drug product of the exploitation that succeeded has Sophora Tankinensis, GUILIN XIGUA SHUANG, nasopharynx spirit
Piece, the fiery ball of clearing suppression etc..
Because subprostrate sophora medicinal material market demand is big, supply falls short of demand for raw material, the face of Guangxi Region in recent years artificial cultivation subprostrate sophora
Product is constantly expanding.Subprostrate sophora artificial growth is main based on seed, tissue-cultured seedling breeding, due to a lack of corresponding seed quality evaluation
Method, commercially available seed quality is inferior, more has False and inferior seeds event of cheating the farmers to occur often, causes the medicinal material underproduction or total crop failure.
At present, it is main to go to observe the quality of subprostrate sophora seed quality by naked eyes, how according to the aqueous of subprostrate sophora seed
Rate, mass of 1000 kernel, germination percentage and cleanliness etc. do quantitative description to the quality of subprostrate sophora seed, never suitable method.
The content of the invention
It is an object of the invention to solve at least the above, and provide the advantage that at least will be described later.
It is a still further object of the present invention to provide a kind of evaluation method of subprostrate sophora seed quality, it utilizes subprostrate sophora seed
Moisture content, mass of 1000 kernel, germination percentage, the seed quality of Purity assessment subprostrate sophora seed lot of seed are criticized, is realized to subprostrate sophora kind
The seed quality of son batch does quantitative assessment, selected evaluation points can effecting reaction seed quality, it is and easy to operate, as a result may be used
Lean on, overcome the blindness and hysteresis quality of conventional judgement.
In order to realize these purposes of the invention and further advantage, there is provided a kind of evaluation of subprostrate sophora seed quality
Method, it is characterised in that comprise the following steps：
Step one, divides seed lot：The same place of production, contemporaneity are harvested, and gross weight is drawn in 1000Kg-20000Kg
Point seed lot, extracts gross weight and is not less than the seed of 1000g and test sample as sending in every batch of seed lot, wherein, the same place of production refers to
The seed for being divided in same seed lot is originated from same small towns, and contemporaneity refers to that the seed is at one month of the same time
Within harvest seed, that is, the time difference for harvesting be no more than one month；
Step 2 selectes evaluation points：Made with seed moisture proportion, thousand grain weigth, percentage of seedgermination and seed purity
It is evaluation points, wherein, seed needed for the measure of seed moisture proportion, thousand grain weigth, percentage of seedgermination and seed purity is equal
Test skewer in sample and take from sending, and be respectively defined as seed I, seed II, seed III and seed IV, seed I, seed II, seed
III and the weight of seed IV be not less than respectively send test sample gross weight 10,1 15,6 percent and
40 percent；
Step 3 determines evaluation points weight：Determine evaluation points seed moisture proportion, thousand grain weigth, percentage of seedgermination,
And the weight of seed purity is respectively 0.185,0.175,0.425 and 0.215；
Step 4 determines the evaluation number of evaluation points：
The moisture content of seed I is measured for a, by formula：Calculate the evaluation of seed lot seed moisture proportion
Index, wherein, a>When 0.75, using A=0 as the evaluation number of seed lot thousand grain weigth；
The mass of 1000 kernel of seed II is measured for b, works as b>During 160g, using B=1 as the evaluation number of seed lot thousand grain weigth,
As b≤160g, by formula：Calculate the evaluation number of seed lot thousand grain weigth；
The germination percentage of seed III is measured for C, using C as the evaluation number of seed lot percentage of seedgermination；
The purity of seed IV is measured for D, using D as the evaluation number of seed lot seed purity；
Step 5 determines subprostrate sophora seed quality rank：By formula：PI=0.185A+0.175B+0.425C+0.215D,
Calculation Estimation desired value；
When PI >=0.95, the seed lot seed quality is excellent, as 0.85≤PI<Seed lot seed quality when 0.95
For good etc., as 0.75≤PI<The seed lot seed quality is medium when 0.85, as 0.65≤PI<Seed lot seed when 0.75
Quality is below average, works as PI<The seed lot seed quality is difference etc. when 0.65.
Preferably, the assay method of the moisture content of seed I is in step 4：It is many parts, every part of kind by the parts by weights of seed I
The weight of son is 4-5g, is W after every part of seed is weighedniBe cut into the thin slice of 3-5mm, after be placed in glass measuring cup, band bottle cap
It is weighed as Wnj, thermostatic drying chamber is placed in after removing bottle cap, after regulating thermostatic drying box temperature dries 8h for 102-105 DEG C, cover bottle
Band bottle cap is taken out after lid and is weighed as W 'nj, the moisture content for measuring every part of seed by formula isWherein, WniIt is
N parts of seed do not cut into slices before weight, WnjIt is the weight with Bottle and closure, W ' before n-th part of seed dryingnjAfter n-th part of seed drying
Weight with Bottle and closure, HniIt is n-th part of moisture content of seed, by formula：The moisture content of seed I is measured, a is
It is the moisture content of the seed lot seed.
Preferably, the glass measuring cup includes：
It is inverted truncated rectangular pyramids shape bottle；
Multiple box bodys, it is that opened top is set, and bottom surface is the rectangular-shape of network structure, and multiple box bodys are between the upper and lower
In detachable setting with the bottle, and it is parallel to the bottom of the bottle；
Wherein, the thin slice is laid in the box body bottom, bottle cap sealable lid set with the box body, before placing baking oven
Bottle cap is covered on the bottle during weighing, and bottle cap takes out when being placed in baking oven, bottle cap when weighing is taken out from baking oven and is covered on institute
State bottle.
Preferably, the measuring method of the mass of 1000 kernel of seed II is full weight method in step 4, and step is as follows：Seed II is passed through
Several instrument meter numbers obtain the grain number of seed II, and balance obtains the weight of seed II, then by formula：Measure seed II
Mass of 1000 kernel, b is the mass of 1000 kernel of the seed lot seed.
Preferably, the assay method of the germination percentage of seed III is in step 4：It is 50- that seed III is pressed into every part of seed grain number
60 are divided into many parts, and the grain number that every part of seed counting obtains every part of seed is designated as into X by several instrumentni, after every part of Seed sterilization, will
Radicle extending area is uniformly arranged on culture dish upward, is put into illumination box, controls temperature for 25 DEG C, is held in every 24h
Continuous light application time is 8h, and intensity of illumination is 2000LX, and counting within the 16th day every part of seed number of normally germinateing respectively in culture is designated as
Yni, and the seed for going normal germination is removed, the number for counting every part of remaining normal germination of seed respectively at the 20th day is designated as Zni,
By formula：Every part of germination percentage of seed is obtained, by formula：The germination percentage of seed III is calculated,
The as germination percentage of the seed lot seed, wherein, YniIt is that n-th part of seed counts normal germination number, Z on the 16th day in cultureniFor
N-th part of seed is in the 20th day number of the statistics normal germination of remaining seed of culture, cniIt is n-th part of germination percentage of seed.
Preferably, in step 4 the purity of seed IV assay method：Seed IV is counted into obtain seed IV by several instrument
Grain number is M1, determine the grain number M of purebred son in seed IV2, by formula：The purity of seed IV is measured, as the seed
The purity of seed is criticized, wherein, purebred son is measured by mode of appearance method：There are hilum, 7.25mm-11.40mm long, width
The seed of 5.01mm-9.04mm and color with reference colour aberration CA values less than or equal to 1.5, the reference colour of purebred son is black.
And its profile is oval, avette or Long Circle, and kernel texture is complete, surface without incomplete or by major injury,
Hung without deformity or contracting, scalpel cuts rear cotyledon milky open, and nothing is caught an illness, go mouldy or rot discoloration, the necrosis phenomena for producing.
The present invention at least includes following beneficial effect：
1st, the evaluation method of subprostrate sophora seed quality of the invention using subprostrate sophora seed lot seed moisture content, thousand
Weight, germination percentage, the seed quality of Purity assessment subprostrate sophora seed lot, realize and the seed quality of subprostrate sophora seed lot are quantified
Evaluate, selected evaluation points can effecting reaction seed quality, and easy to operate, reliable results overcome the blind of conventional judgement
Mesh and hysteresis quality.
2nd, the moisture of seed is to maintain the weight of seed vitality in the evaluation method of subprostrate sophora seed quality of the invention
Material is wanted, the metabolism of seed can just be smoothly completed only in the presence of water, but the too high breathing that can make seed of moisture is made
With excessively vigorous, and make microorganism amount reproduction, finally greatly shorten the seed storage life-span, moisture is too low, can make kind
Sub- dehydration is dead, and the mass of 1000 kernel of seed is capable of the size and turgor of effecting reaction seed, and the purity of seed can be effective
React effective subprostrate sophora seed proportion.
Further advantage of the invention, target and feature embody part by following explanation, and part will also be by this
The research and practice of invention and be understood by the person skilled in the art.
Brief description of the drawings
Fig. 1 is the subprostrate sophora seed germination rate of different cultivation temperatures of the invention；
Fig. 2 is the subprostrate sophora seed germination rate of illumination and dark culturing under different cultivation temperatures of the invention.
Specific embodiment
The invention discloses a kind of evaluation method of subprostrate sophora seed quality, those skilled in the art can be used for reference in herein
Hold, be suitably modified technological parameter realization.In particular, all similar replacements and change are to those skilled in the art
For be it will be apparent that they are considered as being included in the present invention.The method of the present invention and application have passed through preferably implementation
Example is described, and related personnel can substantially enter in present invention, spirit and scope not departing to method described herein
Row is changed or suitably changed with combining and realizes and apply the technology of the present invention.
With reference to embodiment, the present invention is expanded on further：
Embodiment 1
Step one, divides seed lot：The subprostrate sophora seed that in October, 2015 harvests in Nanning Mashan County, seed to be detected
The weight of the seed lot of quality is 10000Kg, extracts 1000g seeds in the seed lot as sending and tests sample, send and tests sample 4
DEG C, preserved under dry environment；
Step 2 selectes evaluation points：Made with seed moisture proportion, thousand grain weigth, percentage of seedgermination and seed purity
It is evaluation points, wherein, seed needed for the measure of seed moisture proportion, thousand grain weigth, percentage of seedgermination and seed purity is equal
Test skewer in sample and take from sending, and be respectively defined as seed I, seed II, seed III and seed IV, seed I extracts to send tests sample
The 10 of gross weight are 100g, seed II is extracted and send test sample gross weight 1 15 for 150g, the extraction of seed III
Send and test 6 the percent of sample gross weight to test 40 the percent of sample gross weight be 400g for 60g and the extraction of seed IV are sent；
Step 3 determines evaluation points weight：Determine evaluation points seed moisture proportion, thousand grain weigth, percentage of seedgermination,
And the weight of seed purity is respectively 0.185,0.175,0.425 and 0.215；
Step 4 determines the evaluation number of evaluation points：
It is 24 parts by the parts by weights of 100g seeds I, 24 parts of seeds is numbered according to table 1, and weigh every part of kind respectively
Sub- weight is Wni, it is specific that each seed in every part of seed is cut into the thin slice of thickness range 3-5mm again as shown in table 1, and
Glass measuring cup is laid in, band Bottle and bottle cap is weighed as W togethernj, glass measuring cup is placed in thermostatic drying chamber without bottle cap, adjust
After section thermostatic drying chamber temperature dries 8h for 102 DEG C, take out band bottle cap and weigh weight for W 'nj, it is specific as shown in table 1, surveyed by formula
The moisture content for going out every part of seed isIt is specific as shown in table 1, and measurement result display numbering is 13 part
The moisture content of seed is up to 0.372, and numbering is the moisture content minimum 0.345 of 18 this part of seed, and both differ 0.027,
In the allowed band of error, wherein, WniWeight before not cut into slices for n-th part of seed, W 'niIt is the weight after n-th part of seed drying
Amount, HniIt is n-th part of moisture content of seed, by formula：It is 35.5% to measure the moisture content of seed I, and 35.5% is
It is the moisture content of the seed lot seed.
The subprostrate sophora seed moisture proportion of table 1 is determined
It is 35.5% to measure the moisture content of seed I, by formula：Calculate seed lot seed moisture proportion
Evaluation number is 0.99；
Wherein, it is inverted truncated rectangular pyramids shape bottle；
Multiple box bodys, it is that opened top is set, and bottom surface is the rectangular-shape of network structure, and multiple box bodys are between the upper and lower
In detachable setting with the bottle, and it is parallel to the bottom of the bottle；
Wherein, the thin slice is laid in the box body bottom, bottle cap sealable lid set with the box body；
During use, the thin slice is laid in the bottom of the box body 2, using the holder of inversion truncated rectangular pyramids shape bottle 1
Body 2, facilitates the placement and taking-up of box body 2, and the removal of chip moisture is independent of each other in adjacent pack 2, and its bottom surface is set to
Network structure can effectively moisturize the speed of removal, increase the heating surface area of thin slice, place bottle cap when weigh before baking oven
The bottle is covered on, bottle cap takes out when being placed in baking oven, bottle cap when weighing is taken out from baking oven and is covered on the bottle, passed through
Lid closure can effectively avoid what the moisture influence when being taken out from baking oven in air was measured on the bottle
Effect, caused measuring error.
150g seeds II are obtained into the grain number of seed II for 1062 by several instrument meter numbers, then by formula： The mass of 1000 kernel of seed II is measured for 141.2g, 141.2g is thousand of the seed lot seed
Weight, works as 141.2g<During 180g, by formula：0.88 evaluation number for being seed lot thousand grain weigth；
By several instrument by 60g seeds III count the grain number of seed III is designated as 424, and be 53 by every part of seed grain number
8 parts, after every part of Seed sterilization are divided into, radicle extending area is uniformly arranged on culture dish upward, be put into illumination box
It is interior, temperature is controlled for 25 DEG C, the continuous light time is 8h in every 24h, and intensity of illumination is 2000LX, is united respectively within the 16th day in culture
Normally germination number is designated as Y to every part of seed of meterni, it is specific as shown in table 2, and the seed for going normal germination is removed, in the 20th natural gift
The number for not counting every part of remaining normal germination of seed is designated as Zni, it is specific as shown in table 2, by formula：Obtain
Every part of germination percentage of seed, by formula：Calculate the germination percentage of seed III, the as germination of the seed lot seed
Rate, wherein, YniIt is that n-th part of seed counts normal germination number, Z on the 16th day in cultureniIt is that n-th part of seed is being cultivated the 20th day
The number that the remaining seed of statistics normally germinates, cniIt is n-th part of germination percentage of seed, measures the germination percentage of seed III for C, is made with C
It is the evaluation number of seed lot percentage of seedgermination；
The subprostrate sophora percentage of seedgermination of table 2 is determined
The related data drawn according to upper table can draw, the germination percentage of seed III is 0.83, using 0.83 as seed lot
The evaluation number of percentage of seedgermination；
By several instrument by 400g seeds IV count the grain number of seed IV be M1=2834, determine purebred son in seed IV
Grain number M2=2441, by formula：Calculating measures the purity of seed IV is 0.86, as the seed lot seed
Purity D=0.86, using D=0.86 as the evaluation number of seed lot seed purity, wherein, purebred son is entered by mode of appearance method
Row is determined：There are hilum, 7.25mm-11.40mm long, width 5.01mm-9.04mm and color and reference colour aberration CA values to be less than or wait
In 1.5 seed, reference colour is black, and the purebred son of detection is both needed to meet structural integrity, and surface is without incomplete or be subject to
Major injury, is hung without deformity or contracting, and scalpel cuts rear cotyledon milky open, and nothing is caught an illness, discoloration, the necrosis for producing of going mouldy or rot
The requirement of phenomenon.
Step 5 determines subprostrate sophora seed quality rank：By formula：PI=0.185A+0.175B+0.425C+0.215D,
Calculation Estimation desired value, it is specific as shown in table 3；
The subprostrate sophora seed evaluation index value of table 3 is calculated
When PI >=0.95, the seed lot seed quality is excellent, as 0.85≤PI<Seed lot seed quality when 0.95
For good etc., as 0.75≤PI<The seed lot seed quality is medium when 0.85, as 0.65≤PI<Seed lot seed when 0.75
Quality is below average, works as PI<The seed lot seed quality is difference etc. when 0.65；
Because of 0.85≤0.87<0.95, so the subprostrate sophora seed that in October, 2015 harvests in Nanning Mashan County, to be detected
Weight waits seed for the seed quality of the seed lot of 10000Kg for good.
Embodiment 2
Step one, divides seed lot：The subprostrate sophora seed that 09 month in 2014 harvests in Baise Napo County Long He townshiies, it is to be checked
The weight for surveying the seed lot of seed quality is 15000Kg, extracts 1500g seeds in the seed lot as sending and tests sample, send and tests
4 DEG C of sample, preserves under dry environment；
Step 2 selectes evaluation points：Made with seed moisture proportion, thousand grain weigth, percentage of seedgermination and seed purity
It is evaluation points, wherein, seed needed for the measure of seed moisture proportion, thousand grain weigth, percentage of seedgermination and seed purity is equal
Test skewer in sample and take from sending, and be respectively defined as seed I, seed II, seed III and seed IV, seed I extracts to send tests sample
The 10 of gross weight are 150g, seed II is extracted and send test sample gross weight 1 15 for 225g, the extraction of seed III
Send and test 6 the percent of sample gross weight to test 40 the percent of sample gross weight be 600g for 90g and the extraction of seed IV are sent；
Step 3 determines evaluation points weight：Determine evaluation points seed moisture proportion, thousand grain weigth, percentage of seedgermination,
And the weight of seed purity is respectively 0.185,0.175,0.425 and 0.215；
Step 4 determines the evaluation number of evaluation points：
It is 30 parts by the parts by weights of 150g seeds I, 30 parts of seeds is numbered according to table 1, and weigh every part of kind respectively
Sub- weight is Wni, it is specific that each seed in every part of seed is cut into the thin slice of thickness range 3-5mm again as shown in table 4, and
Glass measuring cup is laid in, band Bottle and bottle cap is weighed as W togethernj, glass measuring cup is placed in thermostatic drying chamber without bottle cap, adjust
After section thermostatic drying chamber temperature dries 8h for 102 DEG C, take out band bottle cap and weigh weight for W 'nj, it is specific as shown in table 4, surveyed by formula
The moisture content for going out every part of seed isIt is specific as shown in table 4, and measurement result display numbering is 10 part
The moisture content of seed is up to 0.303, and numbering is the moisture content minimum 0.283 of 18 this part of seed, and both differ 0.02,
In the allowed band of error, wherein, WniWeight before not cut into slices for n-th part of seed, W 'niIt is the weight after n-th part of seed drying
Amount, HniIt is n-th part of moisture content of seed, by formula：It is 29.1% to measure the moisture content of seed I, and 29.1% is
It is the moisture content of the seed lot seed.
The subprostrate sophora seed moisture proportion of table 4 is determined
The moisture content of seed I is measured for a=29.1%, by formula：Therefore using 0.83 as plant
The evaluation number of son batch seed moisture proportion；
225g seeds II are obtained into the grain number of seed II for 1803 by several instrument meter numbers, then by formula： The mass of 1000 kernel of seed II is measured for 124.8g, 124.8g is thousand of the seed lot seed
Weight, works as 124.8g<During 180g, by formula：0.78 evaluation number for being seed lot thousand grain weigth；
By several instrument by 90g seeds III count the grain number of seed III is designated as 721, be 13 parts by III point of seed, wherein
1-11 parts is 55 distribution by every part of seed grain number, and the 12nd, 13 part is 58 distribution by every part of seed grain number, and every part of seed goes out
After bacterium, radicle extending area is uniformly arranged on culture dish upward, be put into illumination box, control temperature for 25 DEG C, often
The continuous light time is 8h in 24h, and intensity of illumination is 2000LX, in culture every part of seed of statistics normally germination respectively in the 16th day
Number scale is Yni, it is specific as shown in table 5, and the seed for going normal germination is removed, counting every part of remaining seed respectively at the 20th day just
Often the number of germination is designated as Zni, it is specific as shown in table 5, by formula：Every part of germination percentage of seed is obtained, by public affairs
Formula：The germination percentage of seed III, the as germination percentage of the seed lot seed are calculated, wherein, YniIt is n-th part of seed
In the 16th day normal germination number of statistics of culture, ZniIt is that n-th part of seed normally germinates in the 20th day statistics residue seed of culture
Number, cniIt is n-th part of germination percentage of seed, the germination percentage of seed III is measured for C, using C commenting as seed lot percentage of seedgermination
Valency index；
The subprostrate sophora percentage of seedgermination of table 5 is determined
| Numbering | Yni | Zni | cni | Numbering | Yni | Zni | cni |
| 1 | 29 | 11 | 0.73 | 8 | 29 | 11 | 0.73 |
| 2 | 29 | 10 | 0.71 | 9 | 32 | 9 | 0.75 |
| 3 | 29 | 11 | 0.73 | 10 | 29 | 11 | 0.71 |
| 4 | 28 | 11 | 0.71 | 11 | 30 | 10 | 0.73 |
| 5 | 29 | 10 | 0.71 | 12 | 31 | 10 | 0.71 |
| 6 | 28 | 11 | 0.71 | 13 | 31 | 11 | 0.72 |
| 7 | 30 | 11 | 0.75 | / | / | / | / |
The related data drawn according to upper table can draw, the germination percentage of seed III is 0.72, using 0.72 as seed lot
The evaluation number of percentage of seedgermination；
By several instrument by 600g seeds IV count the grain number of seed IV be M1=4812, determine purebred son in seed IV
Grain number M2=3657, by formula：Calculating measures the purity of seed IV is 0.76, as the seed lot seed
Purity D=0.76, using D=0.76 as the evaluation number of seed lot seed purity, wherein, purebred sub-definite is：There are hilum, length
The seed of 7.25mm-11.40mm, 5.01mm-9.04mm wide and color and reference colour aberration CA values less than or equal to 1.5；
Step 5 determines subprostrate sophora seed quality rank：By formula：PI=0.185A+0.175B+0.425C+0.215D,
Calculation Estimation desired value, it is specific as shown in table 6；
The subprostrate sophora seed evaluation index value of table 6 is calculated
When PI >=0.95, the seed lot seed quality is excellent, as 0.85≤PI<Seed lot seed quality when 0.95
For good etc., as 0.75≤PI<The seed lot seed quality is medium when 0.85, as 0.65≤PI<Seed lot seed when 0.75
Quality is below average, works as PI<The seed lot seed quality is difference etc. when 0.65；
Because of 0.75≤0.76<0.85,2014 year 09 month was in the weight to be detected that Baise Napo County Long He townshiies harvest
The seed quality of 15000Kg subprostrate sophora seed lots is medium seed.
<The seed lot of subprostrate sophora seed determines experiment>
According to the related request of ISTA, GB/T 3543.2, it is not higher than by the same place of production, contemporaneity results, weight
Seed lot is divided within 20000kg, send the minimum weight for testing sample to be set to 1000g, according to GB/T 3543.2, by determination of moisture
Sample, thousand grain weigth, percentage of seedgermination and varietal purity test sample be not less than to send respectively and test the hundred of sample gross weight
/ ten, 1 15,6 percent and 40 percent.
Subprostrate sophora seed is carried out room temperature storage and 4 DEG C of storage tests respectively, the subprostrate sophora seed life of room temperature storage is found
Vigor declines very fast, and its germination percentage drops to 15% after 9 months, and what 4 DEG C of subprostrate sophora seed vigoies of storage declined more delays
Slowly, germination percentage and initial germination percentage after storing 6 months statistically no significant difference, but germination percentage after 9 months with
Statistically difference is also more significant for initial germination percentage, therefore it is the storage life under 4 DEG C, drying condition to determine sample storage condition
It is 6 months.
<Subprostrate sophora seed moisture proportion assay method determines experiment>
Experiment 1：With reference to the related request of ISTA and GB/T 3543, by the contrast of different oven temperatures and drying time
(131 ± 1 DEG C of constant temperature high, dries 3 h, and 20 min are sampled 1 time for research；103 ± 1 DEG C of low constant temperature, dries 10 h, every 0.5 h samplings one
It is secondary), with reference to seed loss water regulation, show that the Oven Method that subprostrate sophora seed moisture content is determined is 102-104 DEG C, dry 8 h.
<Subprostrate sophora thousand grain weigth assay method determines experiment>
The comparative analysis of full flow method, thousand methods, hundred methods is carried out to subprostrate sophora seed by GB/T 3543, it is determined that suitable
Subprostrate sophora seed weight assay method.Result of the test is as shown in table 7 below：
The subprostrate sophora seed weight measurement result of table 7
(note：Lowercase is 0.05 level of signifiance, and capitalization is 0.01 level of signifiance.)
As shown in Table 7, the result obtained by hundred methods, thousand methods, full flow method is not poor in 5% and 1% level of signifiance
It is different, illustrate to be determined for the weight of subprostrate sophora seed, hundred methods, thousand methods and full flow methods are applicable, because of full flow method operation more
Simplicity can effectively use manpower and material resources sparingly, therefore the selected mass of 1000 kernel that subprostrate sophora seed is determined using full flow method of the present invention.
<Subprostrate sophora percentage of seedgermination assay method determines experiment>
Contrast experiment is set by unitary variant method to determine to cultivate subprostrate sophora seed when subprostrate sophora percentage of seedgermination is determined
Temperature, light application time, intensity of illumination and timing statisticses.
Experiment 1：The determination of germination temperature
Under identical other condition of culture, if 10 DEG C, 15 DEG C, 20 DEG C, 25 DEG C, 30 DEG C, 35 DEG C of 6 constant incubators, press
According to every part of 400 seeds, culture in 6 incubators is respectively placed in, the germination result of seed is as shown in table 8 below under different temperatures；
The different temperatures subprostrate sophora germination result of table 8
| Germination temperature (DEG C) | Show money or valuables one carries unintentionally the time (d) | Statistics germination percentage time (d) | Germination percentage (%) |
| 10 | 33 | 41 | 8cC |
| 15 | 16 | 38 | 86aA |
| 20 | 5 | 21 | 76aA |
| 25 | 6 | 20 | 82aA |
| 30 | 7 | 19 | 75aA |
| 35 | 7 | 20 | 54bB |
(note：Lowercase is 0.05 level of signifiance, and capitalization is 0.01 level of signifiance.)
Be can be seen that by table 8 and Fig. 2 carries out variance analysis to the final germination rate of each temperature, 15 DEG C, 20 DEG C, 25 DEG C,
With the final germination rate under the 4 of 30 DEG C temperature conditionss without significant difference (P ﹥ 0.05), under 35 DEG C and 10 DEG C of temperature conditionss most
Whole germination rate significant difference (P ﹤ 0.05).Observe that Germination Temperature is higher in experiment, the mildew and rot quantity of seed is also more, Germination Temperature
For 10 DEG C when, seed go rotten rate be almost 0.Final germination rate highest under 15 DEG C of constant temperatures, but its required sprout time compared with
It is long, 1 times of sprout time almost needed for 25 DEG C, therefore according to said determination result selected 25 DEG C be subprostrate sophora germination most
Good cultivation temperature.
Experiment 2：The determination of germinating bed
Under identical other condition of culture, 25 DEG C incubated, (BP) between (TP), paper on setting paper, on sand in (TS), sand
(S) 4 germinating bed treatment, different germinating bed result of the tests are shown in Table 9；
The different germinating bed subprostrate sophora germination results of table 9
| Germinating bed | On paper (TP) | Between paper (BP) | On sand (TS) | In sand (S) |
| Germination percentage (%) | 85aA | 79bB | 77bB | 0cC |
(note：Lowercase is 0.05 level of signifiance, and capitalization is 0.01 level of signifiance.)
As can be seen from Table 9, it has germination percentage higher and observes more convenient when on paper as germinating bed, therefore this
The selected paper of invention determines the germination percentage of subprostrate sophora seed as germinating bed.
Experiment 3：Germination light application time test
As seen from Figure 2, under identical other condition of culture, using paper as germinating bed, it is divided into illumination, dark two kinds of bars
Part, if 10 DEG C, 15 DEG C, 20 DEG C, 25 DEG C, 30 DEG C and 35 DEG C 6 incubated incubators.Result of the test shows, in identical sprouting
At a temperature of, germination rate no significant difference (P ﹥ 0.05) of the subprostrate sophora seed under illumination and dark culturing, wherein, at 15-25 DEG C
The germination rate of light culture is slightly above optical culture, and the germination rate of 30 DEG C, 35 DEG C and 10 DEG C of optical culture is slightly above light culture, this
The continuous light time is 8h in the selected every 24h of experiment, and intensity of illumination is that 2000LX is condition of culture.
Experiment 4：Duration of test runs and seedling characterization test
The result of observation experiment 1-3 shows that the Germination Rule of subprostrate sophora seed is：Normally germinateed under the conditions of fixed-illumination
Subprostrate sophora seed, broken in the seed coat (show money or valuables one carries unintentionally) for radicle (primary root) is prominent first, then hypocotyl elongation.Hypocotyl length is to about 1-
During 2mm, skin, hypocotyl gradual change green, cotyledon gradual change green or yellow green are planted in cotyledon abjection kind skin or part abjection.Hypocotyl enters
One step is extended, and cotyledon is opened, and seedling is upright.Radicle further extends, and some seedling have secondary root to grow.
Using paper as germinating bed, the continuous light time is 8h in every 24h, and intensity of illumination is 2000LX, in 25 DEG C of incubators
During middle culture, the peak period that subprostrate sophora seed shows money or valuables one carries unintentionally in 5-10d, seedling peak period in 8-16d, but until 20d just stably
Do not sprout for three days on end, therefore fixed its first gate time is 16d, last gate time (duration of test runs) is 20d, its
In, observation finds that the seedling 99% that cotyledon normally deviates from kind of a skin, radicle growth is not stagnated can develop into normal seedling.
The seedling that makes discovery from observation is divided into following several：
A. whole seedlings：Root system development is good, there is obvious primary root and secondary root, and primary root white is long and thin.Cotyledon
Two panels, cotyledon goes out soil type germination, while there is epicotyl and the hypocotyl of elongation, green.Acrospire two panels, Dan Ye, opposite is led to
Evergreen color or yellow green.
B. the seedling of light defects is carried：Primary root local damage, or growth is slightly slow.Cotyledon local damage, but cotyledon
The half of tissue area or more than half still remain normal function (using 50% rule).
C. the seedling of secondary infection：Seedling has developed, but it is serious rot, it is observed that, inspection is not due to seed and feels in itself
Dye causes, but caused by fungi or bacterial infestation, and can determine that all main constructions still maintain.
D. the seedling being damaged：The construction of seedling is incomplete or by major injury, it is impossible to balanced normal development person.Such as
Seedling does not have primary root, and plumular axis, cotyledon, acrospire and bud missing, rupture, contracting are hung, rot；
E. lopsided or interrupted seedling：Seedling mainly constructs deformity, and development is uneven or grows thin and weak.As primary root is short
Slightly, swelling, very thin, cotyledon and acrospire swelling curling, deformity, discoloration, necrosis, plumular axis depth cracking, serious distortion or bending,
Very thin, seedling does not develop further after cotyledon occurs；
F. rot seedling：The main construction of seedling is caught an illness or is rotted seriously, so that hinder the normal development of seedling.
Wherein, the seedling that above-mentioned a, b, c are referred to is normal germination seedling, and the seedling that d, e, f are referred to is abnormal hair
The seedling of bud.
Although embodiment of the present invention is disclosed as above, it is not restricted to listed in specification and implementation method
With, it can be applied to various suitable the field of the invention completely, for those skilled in the art, can be easily
Other modification is realized, therefore under the universal limited without departing substantially from claim and equivalency range, the present invention is not limited
In specific details and shown here as the legend with description.
Claims (6)
1. a kind of evaluation method of subprostrate sophora seed quality, it is characterised in that comprise the following steps：
Step one, divides seed lot：The same place of production, contemporaneity are harvested, and gross weight is divided in 1000Kg-20000Kg plants
Son batch, extracts gross weight and is not less than the seed of 1000g and test sample as sending in the every batch of seed lot；
Step 2 selectes evaluation points：Using seed moisture proportion, thousand grain weigth, percentage of seedgermination and seed purity as commenting
The valency factor, wherein, seed needed for the measure of seed moisture proportion, thousand grain weigth, percentage of seedgermination and seed purity is from sending
Test skewer in sample to take, and be respectively defined as seed I, seed II, seed III and seed IV, seed I, seed II, seed III,
And the weight of seed IV is not less than send test sample gross weight 10,1 15,6 percent and percentage respectively
40；
Step 3 determines evaluation points weight：Determine evaluation points seed moisture proportion, thousand grain weigth, percentage of seedgermination and
The weight of seed purity is respectively 0.185,0.175,0.425 and 0.215；
Step 4 determines the evaluation number of evaluation points：
The moisture content of seed I is measured for a, by formula：Calculate the evaluation number of seed lot seed moisture proportion；
The mass of 1000 kernel of seed II is measured for b, works as b>During 160g, using B=1 as the evaluation number of seed lot thousand grain weigth, when b≤
During 160g, by formula：Calculate the evaluation number of seed lot thousand grain weigth；
The germination percentage of seed III is measured for C, using C as the evaluation number of seed lot percentage of seedgermination；
The purity of seed IV is measured for D, using D as the evaluation number of seed lot seed purity；
Step 5 determines subprostrate sophora seed quality rank：By formula：PI=0.185A+0.175B+0.425C+0.215D, calculates
Evaluation index value；
When PI >=0.95, the seed lot seed quality is excellent, as 0.85≤PI<The seed lot seed quality is good when 0.95
Deng as 0.75≤PI<The seed lot seed quality is medium when 0.85, as 0.65≤PI<Seed lot seed quality when 0.75
It is below average, works as PI<The seed lot seed quality is difference etc. when 0.65.
2. the evaluation method of subprostrate sophora seed quality as claimed in claim 1, it is characterised in that seed I is aqueous in step 4
The assay method of rate is：It it is many parts by the parts by weights of seed I, every part of weight of seed is 4-5g, is after every part of seed is weighed
WniBe cut into the thin slice of 3-5mm, after be placed in glass measuring cup, band bottle cap be weighed as Wnj, freeze-day with constant temperature is placed in after removing bottle cap
Case, after regulating thermostatic drying box temperature dries 8h for 102-105 DEG C, taking-up band bottle cap is weighed as W ' after covering bottle capnj, surveyed by formula
The moisture content for going out every part of seed isWherein, WniWeight before not cut into slices for n-th part of seed, WnjIt is n-th part
Weight with Bottle and closure, W ' before seed dryingnjIt is the weight with Bottle and closure, H after n-th part of seed dryingniIt is n-th part of seed
Moisture content, by formula：The moisture content of seed I is measured, a is the moisture content of the seed lot seed.
3. the evaluation method of subprostrate sophora seed quality as claimed in claim 2, it is characterised in that the glass measuring cup bag
Include：
It is inverted truncated rectangular pyramids shape bottle；
Multiple box bodys, it is that opened top is set, and bottom surface is the rectangular-shape of network structure, and multiple box bodys are between the upper and lower away from can
Dismounting is set with the bottle, and it is parallel to the bottom of the bottle；
Wherein, the thin slice is laid in the box body bottom, and bottle cap sealable lid sets with the box body, weighed before placement baking oven
When bottle cap be covered on the bottle, when being placed in baking oven bottle cap take out, from baking oven take out weigh when bottle cap be covered on the bottle
Body.
4. the evaluation method of subprostrate sophora seed quality as claimed in claim 1, it is characterised in that 1,000, seed in step 4
The measuring method of weight is full weight method, and step is as follows：Seed II is obtained into the grain number of seed II by several instrument meter numbers, balance obtains seed II
Weight, then by formula：The mass of 1000 kernel of seed II is measured, b is the mass of 1000 kernel of the seed lot seed.
5. the evaluation method of subprostrate sophora seed quality as claimed in claim 1, it is characterised in that seed III germinates in step 4
The assay method of rate is：Seed III is divided into many parts for 50-60 by every part of seed grain number, by several instrument by every part of seed meter
The grain number of several every part of seeds is designated as Xni, after every part of Seed sterilization, radicle extending area is uniformly arranged on culture dish upward,
It is put into illumination box, controls temperature for 25 DEG C, the continuous light time is 8h in every 24h, and intensity of illumination is 2000LX, in training
Support and count within the 16th day every part of seed number of normally germinateing respectively and be designated as Yni, and the seed for going normal germination is removed, in the 20th natural gift
The number for not counting every part of remaining normal germination of seed is designated as Zni, by formula：Obtain every part of germination of seed
Rate, by formula：The germination percentage of seed III, the as germination percentage of the seed lot seed are calculated, wherein, YniIt is n-th
Part seed is in the 16th day normal germination number of statistics of culture, ZniFor n-th part of seed is normal in the 20th day remaining seed of statistics of culture
The number of germination, cniIt is n-th part of germination percentage of seed.
6. the evaluation method of subprostrate sophora seed quality as claimed in claim 1, it is characterised in that the purity of seed IV in step 4
Assay method：By several instrument by seed IV count the grain number of seed IV be M1, determine the grain number M of purebred son in seed IV2,
By formula：The purity of seed IV, the as purity of the seed lot seed are measured, wherein, purebred son passes through mode of appearance
Method is measured：There are hilum, 7.25mm-11.40mm long, width 5.01mm-9.04mm and color and reference colour aberration CA values to be less than
Or the seed equal to 1.5.
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| CN108931631A (en) * | 2018-05-31 | 2018-12-04 | 湖南新汇制药股份有限公司 | A kind of polygonatum cyrtonema seed testing method |
| CN109115955A (en) * | 2018-08-24 | 2019-01-01 | 徐长斌 | A kind of wheat seed quality determining method |
| CN112700174A (en) * | 2021-01-19 | 2021-04-23 | 广州白云山中一药业有限公司 | Screening method and quality evaluation method of quality evaluation indexes of white paeony root sprouts |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108931631A (en) * | 2018-05-31 | 2018-12-04 | 湖南新汇制药股份有限公司 | A kind of polygonatum cyrtonema seed testing method |
| CN109115955A (en) * | 2018-08-24 | 2019-01-01 | 徐长斌 | A kind of wheat seed quality determining method |
| CN112700174A (en) * | 2021-01-19 | 2021-04-23 | 广州白云山中一药业有限公司 | Screening method and quality evaluation method of quality evaluation indexes of white paeony root sprouts |
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