CN106706930A - Kit for detecting Parkinson's disease - Google Patents

Kit for detecting Parkinson's disease Download PDF

Info

Publication number
CN106706930A
CN106706930A CN201710113949.6A CN201710113949A CN106706930A CN 106706930 A CN106706930 A CN 106706930A CN 201710113949 A CN201710113949 A CN 201710113949A CN 106706930 A CN106706930 A CN 106706930A
Authority
CN
China
Prior art keywords
antibody
seq
chain variable
variable district
disease
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201710113949.6A
Other languages
Chinese (zh)
Other versions
CN106706930B (en
Inventor
申冬昌
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Jiangsu Nuomingzhe Natural Medicine Laboratory Co ltd
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN201710113949.6A priority Critical patent/CN106706930B/en
Publication of CN106706930A publication Critical patent/CN106706930A/en
Application granted granted Critical
Publication of CN106706930B publication Critical patent/CN106706930B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • G01N33/6893Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to diseases not provided for elsewhere
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/577Immunoassay; Biospecific binding assay; Materials therefor involving monoclonal antibodies binding reaction mechanisms characterised by the use of monoclonal antibodies; monoclonal antibodies per se are classified with their corresponding antigens
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2317/00Immunoglobulins specific features
    • C07K2317/50Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/435Assays involving biological materials from specific organisms or of a specific nature from animals; from humans
    • G01N2333/475Assays involving growth factors
    • G01N2333/515Angiogenesic factors; Angiogenin
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2800/00Detection or diagnosis of diseases
    • G01N2800/28Neurological disorders
    • G01N2800/2835Movement disorders, e.g. Parkinson, Huntington, Tourette

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Peptides Or Proteins (AREA)

Abstract

The invention relates to a kit for detecting Parkinson's disease. The kit comprises an antibody capable of being used for specially detecting the Parkinson's disease, wherein the antibody has a good combination constant. Compared with a kit in the prior art, the kit provided by the invention has higher detection accuracy.

Description

A kind of kit for parkinsonism detection
Technical field
The present invention relates to detection field, and in particular to a kind of kit for parkinsonism detection.
Background technology
Parkinson's (PD) also known as shaking plasy, are one of most common nerve degenerative diseases.Epidemiology shows, suffers from Sick rate is 15~3,28/,100,000 populations, 65 years old crowd about 1% of >;The incidence of disease is 10~21/,100,000 populations/year.The PD causes of disease and hair Interpretation of the cause, onset and process of an illness system is not yet clear and definite, may be relevant with social factor, drug factors, patients factors etc..PD pathological changes are:Substantia nigra of midbrain is caused Compact part, Neurons of Locus Coeruleus depigmentation, black substance pigment is thin out and Lewy body occurs.PD nerve biochemical change be:Substantia nigra of midbrain Compact part, Neurons of Locus Coeruleus depigmentation cause dopamine (DA) at above-mentioned position and its nerve endings to reduce, and (DA is produced when reducing >=70% Raw PD clinical manifestations), and acetylcholine (ACH) effect in nigrostriatum system with DA function antagonisms is relative hyperfunction, DA and ACH dysequilibriums.
So far, the cause of disease of pd is still unclear.Current research trend in age ageing, genetic predisposition and environment The composite factors such as the contact of toxin are relevant.
1) age ageing:
2) environmental factor:Epidemiology survey result finds that the illness rate of Parkinson's has regional disparity, so people Some poisonous materials are there may be in suspection environment, the neuron of brain has been damaged.
3) Familial Occurrence:Physicians have found that Parkinson's seem have the tendency of Familial aggregation in long-term practice, There is the incidence of disease of its relatives of the family of Parkinsonian higher compared with normal population.
4) genetic predisposition:Although the generation of Parkinson's is relevant with aging and environmental toxin, simultaneously not all is old People or exposure or even are equally sucked the people of a large amount of mptp and Parkinson's can all occur with the people of same environment.Although Parkinson Patient also has family aggregation, but does not also find clear and definite Disease-causing gene in the Parkinsonian for distributing so far, The cause of disease for illustrating Parkinson's is multifactor.
In sum, the cause of disease of the explanation pd that any single factor can not be satisfactory.Most researchers tend to handkerchief gold The cause of disease of gloomy disease is the above-mentioned coefficient result of each factor.I.e. after middle age, the individuality susceptible to environmental toxin is being touched After toxin, because of its function of detoxification obstacle, there is subclinical black substance infringement, aggravate with advancing age, dopaminergic god Through the gradual continuous dead denaturation of unit, there are the clinical symptoms of Parkinson's in final decompensation.
At present, the biochemical markers research on Parkinson's early diagnosis sets and immunology, inflammatory reaction, oxidation Common nucleoprotein (a-Syn) the most Research Prospects of the multiple fields such as stress reaction, natural death of cerebral cells, wherein W cynapses.Histopathology is ground Study carefully display, the common nucleoprotein abnormal aggregation of Parkinsonian's nigrostriatum cynapse, deposition and functional disturbance, and cynapse core altogether Albumen is the main component of Lewy body, if therefore detecting cynapse core egg altogether in the body fluid such as cerebrospinal fluid, peripheral blood or saliva Albumen, then judge that subject suffers from parkinsonism.But W albumen lacks specific and sensitive for the detection method of disease marker Property.
Angiopoietin-like 4 albumen (ANGPTL4) is the member of the angiopoietin families of secretory protein.Angiogenin The conservative region of family includes helical region and C-terminal fibrinogen (FBN) spline structure domain.See for example, Kim et al., Biochem.J.346=603-610 (2000).Other members of the family include angiogenin I, ANG2 and blood Pipe generation element 3.Ang-1, ANG2 and 3/ angiogenin of angiogenin 4 combine Tie2 acceptors.See example Such as, Davis et al., Ce 1187,1161-1169 (1996);Maisonpierre et al.,Science277,55-60 (1997);Valenzuela et al,Proc.Natl.Acad.Sc1.USA96,1904-1909(1999);With United States Patent (USP) 5, 521,073;5,650,490;With 5,814,464.Angiogenin I and 4 is the activator of Tie2 acceptors, and ANG2 It is the antagonist (and possible activator) of Tie acceptors with 3.See for example, Folkman&D ' Amore, Cell, 87:1153- 1155(1996);Suri et al., Cell, 87:1171-1180(1996);Masionpierre etal.,Science277: 55-60(1997);With Ward&Dumont, Seminars in Cell&DevelopmentalBiology, 13:19-27(20 02).Tie2 acceptors belong to endothelial cell specific receptor tyrosine kinase family, and it also includes Tiel orphan receptors.The family The protein binding Integrin ανβ3 of another member's angiopoietin-like 3 is shown in for example, the He of US patent applications 20030215451 Camenisch et al.,J.Biol.Chem.,277(19):17281-17290(2002)。
ANGPTL4 also is known as other terms.For example, ANGPTL4 also be known as liver fibrinogen/angiogenin- GAP-associated protein GAP (HFARP) (Kim et al., Biochem.J.346=603-610 (2000)), PPAR Y angiogenins are related Albumen (PGAR) (Yoon, et al., Mol.CellBiol., 20:5343-5349 (2000)), and the moon purport fat that fasting is induced The factor (fasting induced adiposefactor) (FIAF) (Kerten et al., J.Biol.Chem., 275: 28488-28493(2000))。
The in vitro and in vivo research of ANGPTL4 and it is qualitative for therapeutic agent and/or treatment provide it is valuable identification with It was found that, the therapeutic agent and/or treatment can be used to prevent, and alleviate or correct the disease related to ANGPTL4 activity and/or expression Or dysfunction.For example, the mouse of Study on tissue culture and genetic modification has been found to be the bioprocess related to human disease Feature further investigation priceless instrument, the disease includes immunology, and Cancerous disease, Neurobiology, angiocarpy is raw Thing disease, fat and other diseases.Needs find and understand many biological functions of ANGPTL4.
Therefore it is urgently to solve to find a kind of gene marker that can effectively occur in early stage i.e. diagnosable parkinsonism Problem certainly.
The content of the invention
In one aspect of the invention, there is provided the purposes of PP1158 4 its specific antibody, it is used for system The diagnostic reagent or kit of standby detection parkinsonism.
The present invention additionally provides a kind of method for identifying relation between PP1158 4 and parkinsonism.
The present invention additionally provides a kind of antibody of PP1158 4, ABB:The weight chain variable district of the antibody is such as SEQ ID NO:Shown in 1, the light chain variable district such as SEQ ID NO of the antibody:Shown in 2.
The present invention additionally provides the antibody of the improved PP1158 4 with enhanced binding characteristic, its point It is not:
ABB1:The weight chain variable district of the antibody such as SEQ ID NO:Shown in 3, the light chain variable district such as SEQ of the antibody ID NO:Shown in 4.
ABB2:The weight chain variable district of the antibody such as SEQ ID NO:Shown in 5, the light chain variable district such as SEQ of the antibody ID NO:Shown in 6.
ABB3:The weight chain variable district of the antibody such as SEQ ID NO:Shown in 7, the light chain variable district such as SEQ of the antibody ID NO:Shown in 8.
ABB4:The weight chain variable district of the antibody such as SEQ ID NO:Shown in 9, the light chain variable district such as SEQ of the antibody ID NO:Shown in 10.
ABB5:The weight chain variable district of the antibody such as SEQ ID NO:Shown in 11, the light chain variable district such as SEQ of the antibody ID NO:Shown in 12.
ABB6:The weight chain variable district of the antibody such as SEQ ID NO:Shown in 13, the light chain variable district such as SEQ of the antibody ID NO:Shown in 14.
ABB7:The weight chain variable district of the antibody such as SEQ ID NO:Shown in 15, the light chain variable district such as SEQ of the antibody ID NO:Shown in 16.
ABB8:The weight chain variable district of the antibody such as SEQ ID NO:Shown in 17, the light chain variable district such as SEQ of the antibody ID NO:Shown in 18.
ABB9:The weight chain variable district of the antibody such as SEQ ID NO:Shown in 19, the light chain variable district such as SEQ of the antibody ID NO:Shown in 20.
ABB10:The weight chain variable district of the antibody such as SEQ ID NO:Shown in 21, the light chain variable district of the antibody is such as SEQ ID NO:Shown in 22.
ABB11:The weight chain variable district of the antibody such as SEQ ID NO:Shown in 23, the light chain variable district of the antibody is such as SEQ ID NO:Shown in 24.
The present invention is detected using screening technique to parkinsonism first, test result indicate that angiogenin There is obvious up-regulated expression in parkinsonism in sample albumen 4.
In addition, expression of the present invention to PP1158 4 in parkinson's syndrome patient blood is determined Amount detection.Research confirms that PP1158 4 can be as the diagnosis marker of parkinson's syndrome, while can also make To prevent and treat the medicine of parkinson's syndrome.
Beneficial effect:The present invention is screened by lot of experiments, test result indicate that PP1158 4 is in handkerchief There is obvious up-regulated expression in the gloomy syndrome of gold, can be used as parkinson's syndrome disease clinical diagnosis mark.And the present invention grinds Study carefully result and show that the expression by detecting PP1158 4 can be parkinson's syndrome preventive assessment or therapeutic scheme.
Brief description of the drawings
Fig. 1 is the statistical analysis figure of the concentration of ANGPTL4 in blood samples of patients and healthy control group blood.★ refers to P<0.05.
Fig. 2 is specificity and the sensitivity of ROC curve display protein diagnostic parkinsonism.
Specific embodiment
The specific embodiment that the present invention is provided is elaborated below in conjunction with the accompanying drawings.
Embodiment 1
Compared by the batch of early stage genomic data, by 50 normal persons and 40 protein expression data of patient point Analysis, it is found by the applicant that ANGPTL4 albumen is overexpression in disturbances in patients with Parkinson disease.
The expression of mRNA and albumen in all patients and normal population blood is detected by the method for quantitative PCR, is led to Cross measurement result as shown in table 1 below:
MRNA (relative expression quantity) Protein concentration (ng/mL)
Patient 1.7 110.2
Normal population 1 40.5
The above results show that ANGPTL4 high level expressions in the blood of parkinsonism patient point out ANGPTL4 Expression it is closely related with the course of disease of disturbances in patients with Parkinson disease.
ANGPTL4 concentration and disease association in the serum of embodiment 2
Using the monoclonal antibody ABB (weight chain variable district of the antibody such as SEQ ID NO of anti-ANGPTL4:Shown in 1, institute State the light chain variable district such as SEQ ID NO of antibody:Shown in 2), 100 handkerchiefs have detected by ELISA method (double antibody sandwich method) The concentration of ANGPTL4 in golden gloomy syndrome patient and 40 healthy control group serum.Result shows, in patients serum The concentration of ANGPTL4 is 110.4 ± 15.3ng/ml (average ± SD);The concentration of ANGPTL4 is in healthy control group serum 40.6 ± 10.2ng/ml (average ± SD);ANFPRL4 concentration is apparently higher than control group serum (P < 0.05) in patients serum, Referring to Fig. 1.
In addition, being 10.25ng/ml, its sensitiveness and spy according to the cut-off values that Fig. 2 ROC curves set ANGPTL4 The opposite sex is all higher than 90%, and positive rate is 92.5% (37/40).Statistic analysis result shows that ANGPTL4 concentration is golden with handkerchief in blood Gloomy patient is closely related, neutralizes above-mentioned experimental study, it can be deduced that conclusion, secretory ANGPTL4 high scores in disturbances in patients with Parkinson disease Secrete, the particularly preferred mark that can be distinguished as parkinsonism and normal population.
The preparation of the kit of embodiment 3
Reagent preparation box, wherein antibody are the anti-monoclonal antibody ABB of mouse, label and specification.
Use above-mentioned detection kit, by ELISA method quantitative determination unknown blood sample (80,40 is normal population, 40 be disturbances in patients with Parkinson disease) in ANGPTL4 content.
When positive threshold value takes 102.5ng/mL, there are 38 evaluating samples for disturbances in patients with Parkinson disease, wherein 38 by verifying, Patient is, illustrates that accuracy is very high.
When positive threshold value takes 130ng/mL, there are 39 evaluating samples for disturbances in patients with Parkinson disease, wherein 39 by verifying, It is patient, illustrates that accuracy is more increased.
Improvement of the embodiment 4 for antibody performance
By experiment, screening, inventor obtains 10 new antibody, and antibody binding domain or its fragment can be according to The sequence known is produced using method known to those skilled in the art.The affinity of described antibody is entered by radioimmunoassays Row is determined.The sequence of the antibody is as follows respectively:
ABB:The weight chain variable district of the antibody such as SEQ ID NO:Shown in 1, the light chain variable district such as SEQ of the antibody ID NO:Shown in 2.
ABB1:The weight chain variable district of the antibody such as SEQ ID NO:Shown in 3, the light chain variable district such as SEQ of the antibody ID NO:Shown in 4.
ABB2:The weight chain variable district of the antibody such as SEQ ID NO:Shown in 5, the light chain variable district such as SEQ of the antibody ID NO:Shown in 6.
ABB3:The weight chain variable district of the antibody such as SEQ ID NO:Shown in 7, the light chain variable district such as SEQ of the antibody ID NO:Shown in 8.
ABB4:The weight chain variable district of the antibody such as SEQ ID NO:Shown in 9, the light chain variable district such as SEQ of the antibody ID NO:Shown in 10.
ABB5:The weight chain variable district of the antibody such as SEQ ID NO:Shown in 11, the light chain variable district such as SEQ of the antibody ID NO:Shown in 12.
ABB6:The weight chain variable district of the antibody such as SEQ ID NO:Shown in 13, the light chain variable district such as SEQ of the antibody ID NO:Shown in 14.
ABB7:The weight chain variable district of the antibody such as SEQ ID NO:Shown in 15, the light chain variable district such as SEQ of the antibody ID NO:Shown in 16.
ABB8:The weight chain variable district of the antibody such as SEQ ID NO:Shown in 17, the light chain variable district such as SEQ of the antibody ID NO:Shown in 18.
ABB9:The weight chain variable district of the antibody such as SEQ ID NO:Shown in 19, the light chain variable district such as SEQ of the antibody ID NO:Shown in 20.
ABB10:The weight chain variable district of the antibody such as SEQ ID NO:Shown in 21, the light chain variable district of the antibody is such as SEQ ID NO:Shown in 22.
ABB11:The weight chain variable district of the antibody such as SEQ ID NO:Shown in 23, the light chain variable district of the antibody is such as SEQ ID NO:Shown in 24.
Antibody Designation Equilibrium associations constant
ABB 3.23×10-8M
ABB1 2.21×10-10M
ABB2 3.02×10-10M
ABB3 3.37×10-10M
ABB4 2.51×10-10M
ABB5 2.40×10-10M
ABB6 3.01×10-10M
ABB7 1.96×10-10M
ABB8 2.22×10-10M
ABB9 2.05×10-10M
ABB10 2.57×10-10M
ABB11 6.33×10-9M
As can be seen from the above table, the affinity of the new antibody for providing is of about 10-10M, with more preferable affinity.
The antibody is used into identical kit in embodiment 3 to prepare, corresponding antibody is replaced respectively, sent out by testing It is existing, identical Detection results are fully achieved, and also the concentration of antibody all reduces 20% accordingly, can equally reach Identical using effect, this is also illustrated, described antibody activity is improved compared with original antibodies activity.
The preferred embodiments of the present invention are these are only, is not intended to limit the invention, for those skilled in the art For member, all any modification, equivalent substitution and improvements done within the spirit and principles in the present invention etc. should be included in this Within the protection domain of invention.
Sequence table
The > Shens winters of < 110 are prosperous
A kind of kits for parkinsonism detection of the > of < 120
〈210〉1
〈211〉138
〈212〉PRT
The > artificial sequences of < 213
〈400〉ABB
MetGlyTrpSerTrpIlePheLeuPheLeuSerGluThrAlaGlyValLeuSerGluValGlnLeuGlnGlnS erGlyProGluLeuMetLysProGlyAlaSerValLysMetSerCysArgThrSerGlyTyrThrThrPheThrAsp TyrSerIleHisTrpValLysGlnSerHisGlyLysArgLeuGluTrpIleGlyTyrIleAsnProTyrAsnGlyAs pThrTyrCysAspGlnAsnPheLysGlyLysAlaThrLeuThrPheAsnLysAlaSerSerThrAlaTyrMetGluI leProArgLeuThrSerAspAspSerAlaValTyrTyrCysThrArgTrpLysThrIleGlnAlaProPheAlaTyr TrpGlyGlnGlyThrLeuValThrValSerAla
〈210〉2
〈211〉129
〈212〉PRT
The > artificial sequences of < 213
〈400〉ABB
MetAspMetArgAlaProAlaGlnPheLeuGlyIleLeuLeuLeuTrpPheProGlyAlaArgCysGluIleG lnMetThrGlnSerProSerSerMetSerAlaSerLeuGlyAspArgIleThrIleThrCysGlnAlaThrGlnAsp IleValLysAsnLeuAsnTrpTyrGlnGlnLysProGlyLysProProSerPheLeuIleHisTyrAlaThrGluLe uAlaGluGlyValProSerArgPheSerGlySerGlySerGlySerAspTyrSerLeuThrIleSerAsnLeuGluS erGluAspPheAlaAspTyrTyrCysLeuGlnSerTyrAspPheProTyrThrPheGlyGlyGlyThrLysLeuGlu IleAsn
〈210〉3
〈211〉138
〈212〉PRT
The > artificial sequences of < 213
〈400〉ABB1
MetGlyTrpSerTrpIlePheLeuPheLeuSerGluThrAsnGlyValLeuSerGluValGlnAsnGlnGlnS erGlyProGluLeuMetLysProGlyAlaSerValLysMetSerCysArgThrSerGlyTyrThrThrPheThrAsp TyrSerIleHisTrpValLysGlnSerHisGlyLysArgLeuGluTrpIleGlyGlyIleAsnAsnTyrAsnGlyAs pThrTyrCysAsnGlnAsnPheLysGlyLysAlaThrLeuThrPheAsnLysAlaSerSerThrAlaTyrMetGluI leProArgLeuThrSerAspAspSerAlaValPheGlyCysThrArgGlyLysThrIleGlnAlaProPheAlaTyr TrpGlyGlnGlyThrLeuPheThrValSerAla
〈210〉4
〈211〉129
〈212〉PRT
The > artificial sequences of < 213
〈400〉ABB1
MetAspMetArgAlaProAlaGlnPheLeuGlyIleLeuLeuLeuTrpPheAspGlyAlaArgCysGluIleG lnMetThrGlnSerProSerSerMetSerAlaSerLeuGlyAspArgIleThrIleThrCysGlnAlaThrGlnAsp IleValLysAsnLeuAsnAspTyrGlnGlnLysProGlyLysProProSerPheLeuIleHisTyrAlaThrGluLe uAlaGluGlyValProSerArgPheSerSerSerGlySerGlySerAspTyrSerLeuThrIleSerAsnLeuGluS erGluAspPheAlaAspTyrTyrCysLeuSerSerTyrAspPheProTyrThrPheGlyGlyGlyThrLysLeuGlu IleAsn
〈210〉5
〈211〉138
〈212〉PRT
The > artificial sequences of < 213
〈400〉ABB2
MetGlyTrpSerTrpIlePheLeuPheLeuSerGluThrAlaGlyIleLeuSerGluValGlnLeuIleGlnS erGlyProGluLeuMetLysProGlyAlaSerValLysMetSerCysArgThrSerGlyTyrThrThrPheThrAsp TyrSerIleHisTrpValLysGlnSerHisGlyLysArgLeuGluTrpIleIleTyrIleAsnProTyrIleGlyAs pThrTyrCysAspGlnAsnPheLysGlyLysAlaThrLeuThrPheAsnLysAlaSerSerThrAlaTyrMetGluI leIleArgLeuThrSerAspAspSerAlaValTyrIleCysThrArgTrpLysThrIleGlnAlaProPheAlaTyr TrpGlyGlnGlyThrLeuValThrValSerAla
〈210〉6
〈211〉129
〈212〉PRT
The > artificial sequences of < 213
〈400〉ABB2
MetAspMetArgAlaProAlaGlnPheLeuGlyIleLeuLeuLeuTrpPheProGlyAlaArgCysGluIleG lnMetThrAlaSerProSerSerMetSerAlaSerLeuGlyAspArgIleThrIleThrCysGlnAlaThrGlnAsp IleValLysAsnLeuAsnAlaTyrGlnGlnLeuProGlyLysProProSerPheLeuIleHisTyrAlaThrGluLe uAlaGluGlyValProSerArgPheSerGlySerGlySerGlySerAspTyrSerLeuThrIleSerAsnLeuGluS erGluAspPheAlaAspTyrTyrCysLeuGlnSerTyrLeuPheProTyrThrPheGlyGlyGlyThrLysLeuGlu IleAsn
〈210〉7
〈211〉138
〈212〉PRT
The > artificial sequences of < 213
〈400〉ABB3
MetGlyTrpSerTrpIlePheLeuPheLeuSerGluThrAlaGlyValLeuSerTrpValGlnLeuGlnGlnS erGlyProGluLeuMetLysProTrpAlaSerValLysMetSerCysArgThrSerTrpTyrThrThrPheThrAsp TyrSerIleHisTrpValLysGlnSerHisGlyLysArgLeuGluTrpIleGlyTyrIleAsnProTyrTrpGlyAs pThrTyrCysAspGlnAsnPheLysGlyLysAlaThrTrpThrPheAsnLysAlaTrpSerThrAlaTyrMetGluI leProArgLeuThrSerAspAspSerTrpValTyrTyrCysThrArgTrpLysThrIleGlnAlaProPheAlaTyr TrpGlyGlnGlyThrLeuValThrValSerAla
〈210〉8
〈211〉129
〈212〉PRT
The > artificial sequences of < 213
〈400〉ABB3
MetAspMetArgAlaProAlaGlnPheLeuGlyIleLeuLeuLeuTrpPheProGlyAlaArgCysGluIleG lnSerThrGlnSerProSerSerMetSerAlaSerLeuGlyAspArgIleThrIleThrCysGlnAlaThrGlnAsp IleValLysAsnLeuAsnTrpTyrGlnGlnLysProGlyLysProProSerPheLeuIleHisTyrAlaThrGluLe uAlaGluGlyValProSerArgPheSerGlyAspSerSerGlyAspAspTyrSerLeuThrIleSerAsnLeuGluS erGluAspPheAlaAspTyrTyrCysLeuGlnSerTyrAspPheProTyrThrPheGlyGlyGlyThrLysLeuGlu IleAsn
〈210〉9
〈211〉138
〈212〉PRT
The > artificial sequences of < 213
〈400〉ABB4
MetGlyTrpSerTrpIlePheLeuPheLeuSerGluThrAlaGlyValProSerGluValGlnLeuGlnGlnS erGlyProGluLeuMetLysProGlyAlaSerValLysMetSerCysArgThrSerGlyTyrThrThrPheThrAsp TyrSerIleHisTrpValLysGlnSerHisGlyLysArgLeuGluTrpIleGlyTyrIleAsnProTyrAsnGlyAs pThrTyrCysAspGlnAsnPheLysGlyLysAlaThrLeuProPheAsnLysAlaSerProThrAlaTyrMetGluI leProArgLeuThrSerAspAspSerProValTyrTyrCysThrProTrpLysThrIleGlnAlaProPheAlaTyr TrpGlyGlnGlyProLeuValThrValSerAla
〈210〉10
〈211〉129
〈212〉PRT
The > artificial sequences of < 213
〈400〉ABB4
MetAspMetArgAlaProAlaGlnPheLeuGlyIleLeuLeuLeuTrpPheProGlyAlaArgCysGluIleG lnMetThrGlnSerProSerSerMetSerAlaSerLeuGlyAspArgIleThrIleThrCysGlnAlaThrGlnAsp IleValLysAsnLeuAsnLeuTyrGlnGlnSerProGlyLysProProSerPheLeuIleHisTyrAlaSerGluLe uAlaGluGlyValProLeuArgPheSerGlySerGlySerGlySerAspTyrSerLeuThrIleSerAsnLeuGluS erGluAspPheAlaAspTyrTyrCysLeuGlnSerTyrAspPheProTyrThrPheGlyGlyGlyThrLysLeuGlu IleAsn
〈210〉11
〈211〉138
〈212〉PRT
The > artificial sequences of < 213
〈400〉ABB5
MetGlyTrpSerTrpIlePheLeuPheLeuSerGluThrAlaGluValLeuSerGluValGlnLeuGlnGlnS erGlyProGluLeuMetLysGluGlyAlaSerValLysMetSerCysArgThrSerGlyTyrThrThrPheThrGlu TyrSerIleHisTrpValLysGlnSerHisGlyLysArgLeuGluTrpIleGlyTyrIleAsnProTyrAsnGlyAs pThrTyrCysAspGlnAsnPheLysGlyLysAlaThrLeuGluPheAsnLysAlaSerSerThrAlaTyrMetGluI leProArgLeuThrSerAspAspSerAlaValTyrTyrCysThrArgTrpGluThrIleGlnAlaProPheAlaTyr TrpGlyGlnGlyThrLeuValThrValSerAla
〈210〉12
〈211〉129
〈212〉PRT
The > artificial sequences of < 213
〈400〉ABB5
MetAspMetArgAlaProAlaGlnPheLeuGlyIleLeuLeuLeuTrpPheProGlyAlaArgCysGluIleG lnAlaThrGlnSerProSerSerMetSerAlaSerLeuGlyAspArgIleThrIleThrCysGlnAlaThrGlnAsp IleValLysAsnLeuAsnTrpTyrGlnGlnLysProGlyLysProProSerPheLeuIleHisTyrAlaThrGluLe uAlaGluGlyValAlaSerArgPheSerGlySerGlySerGlySerAspTyrSerLeuThrIleSerAsnLeuGluS erGluAspPheAlaAspTyrTyrCysSerGlnSerTyrAspPheProTyrThrPheAlaGlyGlyThrLysLeuGlu IleAsn
〈210〉13
〈211〉138
〈212〉PRT
The > artificial sequences of < 213
〈400〉ABB6
MetGlyTrpSerTrpIlePheLeuPheLeuSerGluThrCysGlyValLeuSerGluCysGlnLeuGlnGlnS erGlyProGluLeuMetLysProGlyAlaSerValLysMetSerCysArgThrSerGlyTyrThrThrPheThrAsp TyrSerIleHisTrpValLysGlnSerHisGlyLysArgLeuGluTrpIleCysTyrIleAsnCysTyrAsnGlyAs pThrTyrCysAspGlnAsnPheLysGlyLysAlaThrLeuThrPheAsnLysAlaSerSerThrAlaTyrMetGluI leCysArgLeuThrSerAspAspSerAlaCysTyrTyrCysThrArgTrpLysThrIleGlnAlaProPheAlaTyr TrpGlyGlnGlyThrLeuValThrValSerAla
〈210〉14
〈211〉129
〈212〉PRT
The > artificial sequences of < 213
〈400〉ABB6
MetAspMetArgAlaProAlaGlnPheLeuGlyIleLeuLeuLeuTrpPheProGlyAlaArgCysGluIleG lnMetThrIleSerProSerSerMetSerAlaSerLeuGlyAspArgIleThrIleThrCysGlnAlaThrGlnAsp IleValLysAsnLeuAsnTrpTyrGlnGlnLysProGlyLysIleProSerPheLeuIleHisTyrAlaThrGluLe uAlaGluGlyValProSerArgPheSerGlySerProSerGlySerAspTyrSerLeuThrIleSerAsnLeuGluS erGluAspPheAlaAspTyrTyrIleLeuGlnSerTyrAspPheProTyrThrPheGlyGlyGlyThrLysLeuGlu IleAsn
〈210〉15
〈211〉138
〈212〉PRT
The > artificial sequences of < 213
〈400〉ABB7
MetGlyTrpSerTrpIlePheLeuPheLeuSerGluThrAlaGlyValLeuGlyGluValGlnLeuGlnGlnS erGlyProGluLeuMetLysProGlyAlaSerValLysMetGlyCysArgThrSerGlyTyrThrThrPheThrAsp TyrSerIleHisTrpValLysGlnSerHisGlyLysArgLeuGluTrpGlyGlyTyrIleAsnProTyrAsnGlyAs pThrTyrCysAspGlnAsnPheLysGlyLysAlaThrLeuThrPheAsnLysAlaSerGlyThrAlaTyrMetGluI leProArgLeuThrSerAspAspSerAlaValTyrTyrCysThrArgTrpLysThrIleGlnAlaProPheAlaTyr TrpGlyGlnGlyThrLeuValThrValSerAla
〈210〉16
〈211〉129
〈212〉PRT
The > artificial sequences of < 213
〈400〉ABB7
MetAspMetArgAlaProAlaGlnPheLeuGlyIleLeuLeuLeuTrpPheProGlyAlaArgCysGluIleA spMetThrGlnSerProSerSerMetSerAlaSerLeuGlyAspArgIleThrIleThrCysGlnAlaThrGlnAsp IleValLysAsnLeuAsnTrpTyrGlnGlnLysProGlyLysProProSerPheLeuIleHisTyrAspThrGluLe uAlaGluAspValProSerArgPheSerSerSerGlySerGlySerAspTyrSerLeuThrIleSerAsnLeuGluS erGluAspPheAlaAspTyrTyrCysLeuGlnSerTyrAspPheProTyrThrPheGlyGlyGlyThrLysLeuGlu IleAsn
〈210〉17
〈211〉138
〈212〉PRT
The > artificial sequences of < 213
〈400〉ABB8
MetGlyTrpSerTrpIlePheLeuPheLeuSerGluThrAlaGlyValLeuSerGluValGlnLeuGlnArgS erGlyProGluLeuMetLysProGlyAlaSerValLysMetSerCysArgArgSerGlyTyrThrThrPheThrAsp TyrSerIleHisTrpValLysGlnSerHisGlyLysArgLeuGluTrpIleGlyTyrIleAsnProTyrArgGlyAs pThrTyrCysAspGlnAsnPheLysGlyLysAlaThrArgThrPheAsnLysAlaSerSerThrAlaTyrMetGluI leProArgLeuThrSerAspAspSerAlaArgTyrTyrCysThrArgTrpArgThrIleGlnAlaProPheAlaTyr TrpGlyGlnGlyThrLeuValThrValSerAla
〈210〉18
〈211〉129
〈212〉PRT
The > artificial sequences of < 213
〈400〉ABB8
MetAspMetArgAlaProAlaGlnPheLeuGlyIleLeuLeuLeuTrpPheAsnGlyAlaArgCysGluIleG lnMetThrGlnSerProSerSerMetSerAlaSerLeuGlyAspArgIleThrIleThrCysGlnAlaThrGlnAsp IleValLysAsnLeuAsnIleTyrGlnGlnLysAsnGlyLysProProSerPheLeuIleHisTyrAlaThrGluLe uAlaGluGlyValProSerArgPheAsnGlySerGlySerGlySerAspTyrSerLeuThrIleSerAsnLeuGluS erAsnAspPheAlaAspTyrTyrCysLeuIleSerTyrAspPheProTyrThrPheGlyGlyGlyThrLysLeuGlu IleAsn
〈210〉19
〈211〉138
〈212〉PRT
The > artificial sequences of < 213
〈400〉ABB9
MetGlyTrpSerTrpIlePheLeuPheLeuSerGluThrTrpGlyValLeuSerGluValGlnLeuGlnGlnS erGlyProGluLeuMetLysProGlyAlaSerValLysMetSerCysArgThrSerGlyTyrThrThrPheThrAsp TyrTrpIleHisTrpValLysGlnSerHisGlyLysArgLeuGluTrpIleGlyTyrIleAsnProTyrAsnGlyAs pThrTyrCysAspGlnAsnPheLysGlyLysAlaThrLeuTrpPheAsnLysAlaSerSerThrAlaTyrMetGluT rpProArgLeuThrSerAspAspSerAlaValTyrTyrCysThrArgTrpLysThrIleGlnAlaProPheAlaTyr TrpGlyGlnGlyThrLeuValThrValSerAla
〈210〉20
〈211〉129
〈212〉PRT
The > artificial sequences of < 213
〈400〉ABB9
MetAspMetArgAlaProAlaGlnPheLeuGlyIleLeuLeuLeuTrpPheProGlyAlaArgCysGluIleG lnMetThrGlnSerProSerSerMetSerAlaSerLeuGlyAspArgIleThrIleThrCysGlnAlaLeuGlnAsp IleValLysAsnLeuLeuTrpTyrGlnGlnLysProGlyLysProProSerPheLeuIleGlyTyrAlaThrGluLe uAlaGluGlyValProSerArgPheLeuGlySerGlyLeuGlySerAspTyrSerLeuThrIleSerAsnLeuGluS erGluAspPheAlaAspTyrTyrCysLeuGlnSerTyrAspPheProTyrThrPheGlyGlyGlyThrLysLeuGlu IleAsn
〈210〉21
〈211〉138
〈212〉PRT
The > artificial sequences of < 213
〈400〉ABB10
MetGlyTrpSerTrpIlePheLeuPheLeuSerGluThrAlaGlyValLeuSerAlaValGlnLeuGlnGlnS erGlyProGluLeuMetLysProGlyAlaSerValLysMetSerCysAlaThrSerGlyTyrThrThrPheThrAsp TyrSerIleHisTrpValLysGlnSerHisGlyLysArgLeuGluTrpIleGlyTyrIleAsnProAlaAsnGlyAs pThrTyrCysAspGlnAsnPheLysGlyLysAlaThrLeuThrPheAsnLysAlaSerSerThrAlaTyrMetGluI leProArgLeuThrSerAspAspSerAlaSerTyrTyrCysThrArgTrpLysThrIleGlnAlaProPheAlaTyr TrpGlyGlnGlyThrLeuValThrValSerAla
〈210〉22
〈211〉129
〈212〉PRT
The > artificial sequences of < 213
〈400〉ABB10
MetAspMetArgAlaProAlaGlnPheLeuGlyIleLeuLeuLeuTrpPheProGlyAlaArgCysGluIleG lnMetGluGlnSerProSerSerMetSerAlaSerLeuGlyAspArgIleThrIleThrCysAsnAlaThrGlnAsp IleValLysAsnLeuAsnTrpTyrGlnGlnLysProGlyLysProProSerPheLeuIleHisTyrAlaThrGluLe uAlaAsnGlyValProSerArgPheSerGluSerGlyGluGlySerAspTyrSerLeuThrIleSerAsnLeuGluS erGluAspPheAlaAspTyrTyrCysLeuGlnSerTyrAsnPheProTyrThrPheGlyGlyGlyThrLysLeuGlu IleAsn
〈210〉23
〈211〉138
〈212〉PRT
The > artificial sequences of < 213
〈400〉ABB11
MetGlyTrpSerTrpIlePheLeuPheLeuSerGluThrAlaGlyValLeuSerGluValGlnGlyGlnGlnS erGlyProGluLeuMetLysProGlyAlaSerValLysMetSerCysArgThrSerGlyTyrThrThrPheThrAsp TyrGlyIleHisTrpValLysGlnSerHisGlyLysArgLeuMetTrpIleGlyTyrIleAsnProTyrAsnGlyAs pThrTyrCysAspGlnAsnPheLysGlyLysAlaThrLeuThrPheAsnLysAlaSerSerThrAlaTyrMetGluI leProArgLeuThrSerAspAspSerAlaValTyrMetCysThrArgTrpLysThrIleGlnAlaProPheAlaTyr TrpGlyGlnGlyThrLeuValThrValSerAla
〈210〉24
〈211〉129
〈212〉PRT
The > artificial sequences of < 213
〈400〉ABB11
MetAspMetArgAlaProAlaGlnPheLeuGlyIleLeuGlyLeuTrpPheProGlyAlaArgCysGluIleG lnMetProGlnSerProSerSerMetSerAlaSerLeuGlyAspArgIleThrIleThrCysGlnGlyThrGlnAsp IleValLysAsnLeuAsnTrpTyrGlnGlnLysProGlyLysProProSerPheLeuIleHisTyrAlaThrGluLe uAlaGluGlyValProSerArgPheSerGlySerGlySerGlySerAspTyrSerLeuThrIleSerAsnLeuGluS erGluAspPheAlaAspTyrTyrCysProGlnSerTyrAspPheProTyrThrPheGlyGlyGlyThrLysLeuGlu IleAsn

Claims (5)

1. it is a kind of for parkinsonism detection kit, it is characterised in that:It contains being capable of specific detection Parkinson The monoclonal antibody of syndrome.
2. kit as claimed in claim 1, it is characterised in that:The monoclonal antibody can specifically bind ANGPTL4.
3. kit as claimed in claim 2, it is characterised in that:The weight chain variable district of wherein described antibody is respectively such as SEQ ID NO:1st, shown in 3,5,7,9,11,13,15,17,19,21,23;The corresponding light chain variable district of the antibody is successively such as SEQ ID NO:2nd, shown in 4,6,8,10,12,14,16,18,20,22,24.
4. a kind of monoclonal antibody, it is characterised in that:The weight chain variable district of the antibody is respectively such as SEQ ID NO:1、3、5、7、 9th, shown in 11,13,15,17,19,21,23;The corresponding light chain variable district of the antibody is successively such as SEQ ID NO:2、4、6、8、 10th, shown in 12,14,16,18,20,22,24.
5. application of the monoclonal antibody described in claim 4 in the kit for preparing detection parkinsonism.
CN201710113949.6A 2017-02-28 2017-02-28 A kind of kit for parkinsonism detection Active CN106706930B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201710113949.6A CN106706930B (en) 2017-02-28 2017-02-28 A kind of kit for parkinsonism detection

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201710113949.6A CN106706930B (en) 2017-02-28 2017-02-28 A kind of kit for parkinsonism detection

Publications (2)

Publication Number Publication Date
CN106706930A true CN106706930A (en) 2017-05-24
CN106706930B CN106706930B (en) 2018-11-02

Family

ID=58917650

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201710113949.6A Active CN106706930B (en) 2017-02-28 2017-02-28 A kind of kit for parkinsonism detection

Country Status (1)

Country Link
CN (1) CN106706930B (en)

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101370525A (en) * 2005-08-19 2009-02-18 艾博特公司 Dual variable domain immunoglobin and uses thereof
CN102692500A (en) * 2011-03-21 2012-09-26 上海市肿瘤研究所 ANGPTL4 as a marker of liver cancer metastasis by serological detection and application thereof

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101370525A (en) * 2005-08-19 2009-02-18 艾博特公司 Dual variable domain immunoglobin and uses thereof
CN102692500A (en) * 2011-03-21 2012-09-26 上海市肿瘤研究所 ANGPTL4 as a marker of liver cancer metastasis by serological detection and application thereof

Also Published As

Publication number Publication date
CN106706930B (en) 2018-11-02

Similar Documents

Publication Publication Date Title
Simcock et al. Proangiogenic activity in bronchoalveolar lavage fluid from patients with asthma
Talley et al. Non-ulcer dyspepsia and duodenal eosinophilia: an adult endoscopic population-based case-control study
Wen et al. Induced dural lymphangiogenesis facilities soluble amyloid-beta clearance from brain in a transgenic mouse model of Alzheimer's disease
CA3071824A1 (en) Biomarkers associated with parkinson&#39;s disease
US10613090B2 (en) Methods of detecting cancer
CN106461677B (en) The inspection method of pulmonary hypertension disease
JP2009515183A5 (en)
CN105092858A (en) Diagnosis and risk stratification of infections and chronic diseases of the respiratory tract and lungs by means of provasopressin
Sone et al. Relevance and characteristics of gastroesophageal reflux in adult patients with otitis media with effusion
Kim et al. TGF-β/SMAD4 mediated UCP2 downregulation contributes to Aspergillus protease-induced inflammation in primary bronchial epithelial cells
Wang et al. Relationship between serum 25‐hydroxyvitamin D3 levels and severity of chronic periodontitis in type 2 diabetic patients: A cross‐sectional study
Tao et al. Capsaicin receptor TRPV1 maintains quiescence of hepatic stellate cells in the liver via recruitment of SARM1
Nongrum et al. Analysing adipokine Omentin-1 in periodontal disease and type-2 diabetes mellitus: An interventional comparative study
Karlsson et al. The Registry for Migraine (REFORM) study: methodology, demographics, and baseline clinical characteristics
CN104977415A (en) Applications and kit of METRNL protein as inflammatory bowel disease diagnostic serum marker
CN106706930B (en) A kind of kit for parkinsonism detection
CN106596979B (en) A kind of kit and its detection method for parkinsonism detection
Xiang et al. ER stress aggravates diaphragm weakness through activating PERK/JNK signaling in obesity hypoventilation syndrome
Wang et al. Similarities and differences in the effects of sensitisation and challenge with Dermatophagoides farinae and Dermatophagoides pteronyssinus extracts in a murine asthma surrogate
Ezzat et al. Serum mucosa‐associated epithelial chemokine (MEC/CCL28) in atopic dermatitis: a specific marker for severity
CN105807067A (en) Dry-type immunofluorescence kit for detecting NCAM2 (neural cell adhesion molecules 2) of patient suffering from alzheimer&#39;s syndromes and preparation method
Kucukosmanoglu et al. Plasma adrenomedullin levels in children with asthma: Any relation with atopic dermatitis?
Ezzat et al. Serum mucosa-associated epithelial chemokine in atopic dermatitis: a specific marker for severity
Phillips Measurement of bioactive neuropeptides using a chromatographic immunosensor cartridge
Feng et al. Upregulated expression of intestinal antimicrobial peptide HD5 associated with renal function in IgA nephropathy

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
TA01 Transfer of patent application right
TA01 Transfer of patent application right

Effective date of registration: 20180910

Address after: 225128 Floors 4 and 5 of Nanyuan Chuangfu Workshop Building 4-2, Yangzhou High-tech Industrial Development Zone, Jiangsu Province (Yongjiang Economic Development Zone 4)

Applicant after: YANGZHOU NUOMING ZHETIAN MEDICAL LABORATORY Co.,Ltd.

Address before: 510000 Guangdong science and Technology Industrial Development Zone, Guangzhou, 231 and 233 podium B1B2 Building 1, two, three, four

Applicant before: BOAO ZONGHENG NETWORK TECHNOLOGY Co.,Ltd.

Effective date of registration: 20180910

Address after: 510000 Guangdong science and Technology Industrial Development Zone, Guangzhou, 231 and 233 podium B1B2 Building 1, two, three, four

Applicant after: BOAO ZONGHENG NETWORK TECHNOLOGY Co.,Ltd.

Address before: 610041 Sichuan University, Sichuan, 17 three South Road, Chengdu.

Applicant before: Shen Dongchang

GR01 Patent grant
GR01 Patent grant
CP03 Change of name, title or address
CP03 Change of name, title or address

Address after: 225000 South Park Chuangfu Workshop Building 4-2, Yangzhou High-tech Industrial Development Zone, Jiangsu Province, 4 and 5 floors (Hanjiang Economic Development Zone 4)

Patentee after: Jiangsu nuomingzhe natural medicine laboratory Co.,Ltd.

Address before: 225128 Floors 4 and 5 of Nanyuan Chuangfu Workshop Building 4-2, Yangzhou High-tech Industrial Development Zone, Jiangsu Province (Yongjiang Economic Development Zone 4)

Patentee before: YANGZHOU NUOMING ZHETIAN MEDICAL LABORATORY Co.,Ltd.