CN106706786A - Method for determining content of six ginsenoside ingredients of folium ginseng - Google Patents
Method for determining content of six ginsenoside ingredients of folium ginseng Download PDFInfo
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Abstract
The invention provides a method for determining content of six ginsenoside ingredients of folium ginseng. Ginsenoside is the main active ingredient of the folium ginseng and an important criterion for measuring the quality of the folium ginseng, but the content is greatly different from that of ginseng. According to the method, ultra-high performance liquid chromatography is used for determining the six ginsenoside ingredients including ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, ginsenoside Rb2, 20(S)-ginsenoside F1 and ginsenoside Rd in the folium ginseng simultaneously, the method is high in sensitivity and accurate, and can be used for quality control of the folium ginseng.
Description
Technical field
The present invention relates to a kind of content assaying method of Ginsenosides in folium panacis japonici cum caule, belong to technical field of traditional Chinese medicines,
Specifically, being related to ginsenoside Rg in a kind of folium panacis japonici cum caule1, ginsenoside Re, ginsenoside Rb1, ginsenoside Rb2, 20 (S)-people
The content assaying method that ginseng saponin(e F 1 and six kinds of compositions of ginsenoside Rd are determined simultaneously.
Background technology
Folium panacis japonici cum caule is the dried leaf of Araliaceae ginseng Panax ginseng C.A.Mey..Autumn harvest, dry or
Drying.Folium panacis japonici cum caule is bitter, sweet, cold, and return lung, stomach two are passed through, first recorded in《A Supplement to the Compendium of Materia Medica》, with " clearing lung-heat, promotes the production of body fluid, and quenches the thirst ", " mend
The function such as middle band table, raw stomach Tianjin, driving away summer heat gas, the drop fire of deficiency type, the sharp four limbs head ", " fluid dryness, beneficial lung and liver, tonifying vital QI ".It is existing
Generation research shows that the pharmacological action of folium panacis japonici cum caule is very much like with ginseng, has preferably to tumour, hepatitis, coronary heart disease, Addison's disease
Therapeutic effect.Meanwhile, folium panacis japonici cum caule can also improve the flexibility of nervous activity process.Can improve the health care of sleep and mood, improve it is mental,
The functions of human body such as muscle power, there is significant antifatigue, diuresis and radiation resistance.Defence of the body to various destructive stimuluses can be increased
Ability.There is certain preventive and therapeutic effect to cardiac muscular dystrophy, coronary sclerosis, neurasthenia etc..Though folium panacis japonici cum caule is good, can not
Abuse, as effect of folium panacis japonici cum caule is gradually well known, gradually has the gesture of abuse, or even cause various toxic and side effects in recent years
Appearance.Science, reasonably turned into very important problem using folium panacis japonici cum caule, therefore, the quality standard of folium panacis japonici cum caule need into
The raising of one step is perfect.
The content of the invention
Object of the present invention is to provide ginsenoside Rg in a kind of folium panacis japonici cum caule1, ginsenoside Re, ginsenoside Rb1、
Ginsenoside Rb2, six kinds of compositions of 20 (S)-GF1s and ginsenoside Rd simultaneously determine content assaying method.
Content assaying method of the present invention uses ultra-performance liquid chromatography, and the method is as follows：
It is micro- warp of filler that chromatographic condition uses octadecylsilane chemically bonded silica with system suitability chromatographic column
Post；With acetonitrile as mobile phase A, water is Mobile phase B, carries out gradient elution, and gradient is：0~9 minute, mobile phase A was by 18%
Gradual change is to 20%；9~13 minutes, mobile phase A was by 20% gradual change to 28%；13~28 minutes, mobile phase A be 28% gradual change extremely
35%；Detection wavelength is 203nm；Flow velocity 0.4ml/min；
The preparation of mixed reference substance solution
Weigh ginsenoside Rg1Reference substance, ginsenoside Re's reference substance, ginsenoside Rb1Reference substance, ginsenoside Rb2It is right
It is appropriate according to product, 20 (S)-GF1 reference substances, ginsenoside Rd's reference substance, it is accurately weighed, plus methyl alcohol is made every 1ml containing people
Ginseng saponin(e Rg1100 μ g, the μ g of ginsenoside Re 500, ginsenoside Rb150 μ g, ginsenoside Rb280 μ g, 20 (S)-ginsengs
The mixed solution of the μ g of saponin(e F1 50, the μ g of ginsenoside Rd 200, obtains final product；
The preparation of need testing solution takes Ginseng Leaf, crosses No. four sieves, and about 0.4g is accurately weighed, in putting conical flask with cover,
Precision adds 60~80% methyl alcohol 25ml, and close plug, weighed weight is put refluxing extraction 2.5~3 hours in 75~82 DEG C of water-baths, put
It is cold, then weighed weight, the weight of less loss is supplied with same concentration methyl alcohol, shake up, filter, subsequent filtrate is taken, obtain final product；
Determination method is accurate respectively to draw reference substance solution and each 1~2 μ l of need testing solution, injects liquid chromatograph, determines,
Content is calculated with external standard method, is obtained final product.
In content assaying method of the present invention, water-bath reflux extracting time is preferably 2.5 hours in prepared by need testing solution；Survey
Determine in method that accurate reference substance solution of drawing is preferably 2 μ l with the amount of need testing solution respectively, inject liquid chromatograph.
The optimal technical scheme of content assaying method of the present invention is：
It is micro- warp of filler that chromatographic condition uses octadecylsilane chemically bonded silica with system suitability chromatographic column
Post；With acetonitrile as mobile phase A, water is Mobile phase B, carries out gradient elution, and gradient is：0~9 minute, mobile phase A was by 18%
Gradual change is to 20%；9~13 minutes, mobile phase A was by 20% gradual change to 28%；13~28 minutes, mobile phase A be 28% gradual change extremely
35%；Detection wavelength is 203nm；Flow velocity 0.4ml/min；
The preparation of mixed reference substance solution
Weigh ginsenoside Rg1Reference substance, ginsenoside Re's reference substance, ginsenoside Rb1Reference substance, ginsenoside Rb2It is right
It is appropriate according to product, 20 (S)-GF1 reference substances, ginsenoside Rd's reference substance, it is accurately weighed, plus methyl alcohol is made every 1ml containing people
Ginseng saponin(e Rg1100 μ g, the μ g of ginsenoside Re 500, ginsenoside Rb150 μ g, ginsenoside Rb280 μ g, 20 (S)-ginsengs
The mixed solution of the μ g of saponin(e F1 50, the μ g of ginsenoside Rd 200, obtains final product；
The preparation of need testing solution takes Ginseng Leaf, crosses No. four sieves, and about 0.4g is accurately weighed, in putting conical flask with cover,
Precision adds 80% methyl alcohol 25ml, and close plug, weighed weight is put refluxing extraction 2.5 hours in 82 DEG C of water-baths, let cool, then weighed heavy
Amount, the weight of less loss is supplied with 80% methyl alcohol, is shaken up, and is filtered, and takes subsequent filtrate, is obtained final product；
Determination method is accurate respectively to draw reference substance solution and each 2 μ l of need testing solution, injects liquid chromatograph, determines, with
External standard method calculates content, obtains final product.
Determine the stability of content method, accuracy, specificity and system suitability to verify, method has been carried out with
Lower methodology confirmatory experiment, to ensure that the content assaying method can be used for the quality control of folium panacis japonici cum caule.
1st, instrument and reagent
Instrument：Acquity Ultra Performance Liquid Chromatography instruments (binary pump, DAD detectors)
The Ultra Performance Liquid Chromatography instruments of Ultimate 3000 (quaternary pump, DAD detectors)
LC20~AT Ultra Performance Liquid Chromatography instruments (binary pump, DAD detectors)
Reference substance：Ginsenoside Rg1 (Zhong Jian institutes, lot number：110703~201530)
Ginsenoside Re (Zhong Jian institutes, lot number：110754~201525)
Ginsenoside Rb1(Zhong Jian institutes, lot number：110704~201424)
Ginsenoside Rb2(Zhong Jian institutes, lot number：111715~201203)
20 (S)-GF1s (Nantong Fei Yu bio tech ltd, lot number：FY15401226)
Ginsenoside Rd (Zhong Jian institutes, lot number：111818~201302)
Reagent：Acetonitrile is chromatographically pure, and water is deionized water, and it is pure that other reagents are analysis.
2. the determination of chromatographic condition
With reference to the ginseng chromatographic condition that Chinese Pharmacopoeia version one in 2015 is recorded, determine that this product chromatographic condition is：With 18
Alkyl silane bonded silica gel is filler (micro- through post)；Gradient elution is carried out by mobile phase of acetonitrile~water, it is true through repetition test
Fixed following gradient elution program (being shown in Table 1).Flow velocity：0.4mL/min, column temperature：30 DEG C, Detection wavelength：203nm, sample size：2μ
l.Number of theoretical plate is calculated by ginsenoside Re peak and should be not less than 10000.
The eluent gradient of table 1 elutes table
3. the determination of extracting method
The selection of 3.1 extracting modes
Take same batch of sample appropriate, choose 80% methyl alcohol as Extraction solvent, ultrasound, two kinds of extraction sides of backflow are respectively adopted
Formula is tested, and is determined by chromatographic condition is drafted.Result shows, by the way of backflow, six kinds of assay results of composition
Ultrasound is above, therefore from the extracting mode (the results are shown in Table 2) that backflow is tested as this.
The assay result of the six kinds of compositions of different extracting modes of table 2
3.2 Extraction solvents are selected
Take same batch of sample appropriate, respectively using methyl alcohol, 80% methyl alcohol, 60% methyl alcohol, 40% methyl alcohol as Extraction solvent, return
Stream extracts 2h.Result shows, ginsenoside Rg1Four kinds of Extraction solvent assay results are without significant difference more than；Ginseng
The content of saponin(e Re slightly rises with the increase of water in Extraction solvent；Ginsenoside Rb1, 20 (S) GF1s and people
Ginseng saponin(e Rb2Content is higher when being extracted with 60% methyl alcohol using 80% methyl alcohol；Ginsenoside Rd is contained when being extracted using 40% methyl alcohol
Amount is minimum, and its excess-three kind solvent difference is too big.Consider based on the above results, from the Extraction solvent that 80% methyl alcohol is tested as this
(the results are shown in Table 3).
The assay result of table 3 different solvents, six kinds of compositions
The selection of 3.3 return times
Take same batch of sample appropriate, reflux extracting time is respectively 0.5,1.0,1.5,2.0,2.5,3.0h, by drafting color
Spectral condition is determined.When result shows that return time is within 2.5h, with the increase of return time, the content for surveying composition is obvious
Increase, and after 2.5h, the assay result of six kinds of compositions does not have significant difference, therefore the selective extraction time is 2.5h.(knot
4) fruit is shown in Table
The assay result of the six kinds of compositions of different extraction times of table 4
By experiment, extracting method is defined as：This product powder (crossing No. four sieves) about 0.4g is taken, it is accurately weighed, put tool plug cone
In shape bottle, precision adds 80% methyl alcohol 25ml, and close plug, weighed weight puts refluxing extraction 2.5 hours in 82 DEG C of water-baths, takes out, and puts
It is cold, then weighed weight, the weight of less loss is supplied with 80% methyl alcohol, shake up, filter, subsequent filtrate is taken, obtain final product.
Reference substance and test sample chromatogram are shown in Fig. 1.As seen from Figure 1, each composition peak shape of Ginsenosides is symmetrical, separates good
It is good, also exist without corresponding Interference Peaks in sample, specificity is preferable.
4. methodological study
4.1 linear investigations
Precision draws the ginsenoside Rg of various concentrations respectively1, ginsenoside Re, ginsenoside Rb1, 20 (S)-ginseng soaps
Glycosides F1, ginsenoside Rb2, ginsenoside Rd's reference substance solution, Ultra Performance Liquid Chromatography instrument is injected separately into, by the color under 2.3
Spectral condition is determined, and with reference substance sample size (μ g) as abscissa, integrating peak areas value is ordinate, draws standard curve.Ginseng
Saponin(e Rg1It is in good linear relationship between 0.01922 μ g~1.922 μ g, regression equation is：Y=895293x+461 (r=
0.9999)；Ginsenoside Re is in good linear relationship between 0.09687 μ g~9.687 μ g, and regression equation is：Y=
775587x+18759 (r=0.9999)；Ginsenoside Rb1It is in good linear relationship between 0.01162 μ g~1.162 μ g,
Regression equation is：Y=644122x+2088 (r=0.9999)；20 (S)-GF1s 0.01230 μ g~1.230 μ g it
Between be in good linear relationship, regression equation is：Y=1044583x+5271 (r=0.9999)；Ginsenoside Rb2
It is in good linear relationship between 0.01164 μ g~1.164 μ g, regression equation is：Y=681704x+1356 (r=0.9999)；
Ginsenoside Rd is in good linear relationship between 0.03802 μ g~3.802 μ g, and regression equation is：Y=760002x+2946
(r=0.9999).(experimental result is shown in Table 5, table 6).
The linear relationship of table 5 investigates result (1)
The linear relationship of table 6 investigates result (2)
4.2 replica tests
Take same folium panacis japonici cum caule's sample (No. 2) appropriate, crush, cross No. four sieves, mix, nominal takes 9 parts, and numbering is 1~9, its
In 1~No. 3 0.2g, 4~No. 6 0.4g, 7~No. 9 0.6g, it is accurately weighed, put respectively in conical flask with cover, test sample is made in accordance with the law
Solution, determines content.7 are the results are shown in Table, shows repeated good.
The replica test result (not rolling over moisture) of table 7
4.3 average recoveries are tested
, crushing appropriate with a collection of folium panacis japonici cum caule's sample (No. 2) is taken, No. four sieves are crossed, mixed, take 9 parts of powder, every part of about 0.2g essence
Close weighed, every three parts is one group, is separately added into the reference substance solution of basic, normal, high three concentration prepared with 80% methanol solution
25ml, by need testing solution preparation lower section legal system available test sample solution.Determined by the chromatographic condition drafted, calculate the rate of recovery.
The results are shown in Table 8~13.Show that this method rate of recovery is preferable.
The ginsenoside Rg of table 81Recovery test result
The ginsenoside Re's recovery test result of table 9
The ginsenoside Rb of table 101Recovery test result
Table 11 20 (S)-ginseng saponin F1Recovery test result
The ginsenoside Rb of table 122Recovery test result
The ginsenoside Rd's recovery test result of table 13
Conclusion：Through experiment, ginsenoside Rg1, ginsenoside Re, ginsenoside Rb1, 20 (S)-GF1s, ginseng soap
Glycosides Rb2, ginsenoside Rd average recovery be respectively 99.3%, 101.8%, 98.3%, 99.95%, 101.0%,
101.4%, RSD are respectively 1.5%, 1.0%, 1.8%, 0.9%, 1.5%, 1.1%, show that each composition average recovery is good
It is good.
4.4 stability tests
Ultra Performance Liquid Chromatography instrument is injected in 0,2,6,10,16, the accurate same need testing solutions of absorption of 24h successively, is determined,
13 are the results are shown in Table, shows need testing solution stabilization at least in 24 hours.
The stability test result of table 13
4.5 sample determinations
The folium panacis japonici cum caule being collected into totally 10 crowdes is taken, is determined, calculate content, the results are shown in Table 14.
The folium panacis japonici cum caule's sample size measurement result of table 14
Brief description of the drawings
Six kinds of Ginsenosides assay chromatogram (peaks 1 in Fig. 1 folium panacis japonici cum caules：Ginsenoside Rg1；Peak 2：Ginseng
Saponin(e Re；Peak 3：Ginsenoside Rb1Peak；4：Ginseng saponin F1；Peak 5：Ginsenoside Rb2；Peak 6：Ginsenoside Rd)；
Specific embodiment
Embodiment 1
It is micro- warp of filler that chromatographic condition uses octadecylsilane chemically bonded silica with system suitability chromatographic column
Post；With acetonitrile as mobile phase A, water is Mobile phase B, carries out gradient elution, and gradient is：0~9 minute, mobile phase A was by 18%
Gradual change is to 20%；9~13 minutes, mobile phase A was by 20% gradual change to 28%；13~28 minutes, mobile phase A be 28% gradual change extremely
35%；Detection wavelength is 203nm；Flow velocity 0.4ml/min；
The preparation of mixed reference substance solution
Weigh ginsenoside Rg1Reference substance, ginsenoside Re's reference substance, ginsenoside Rb1Reference substance, ginsenoside Rb2It is right
It is appropriate according to product, 20 (S)-GF1 reference substances, ginsenoside Rd's reference substance, it is accurately weighed, plus methyl alcohol is made every 1ml containing people
The μ g of ginseng saponin(e Rg1 100, the μ g of ginsenoside Re 500, ginsenoside Rb150 μ g, ginsenoside Rb280 μ g, 20 (S)-ginsenosides
The mixed solution of the μ g of F1 50, the μ g of ginsenoside Rd 200, obtains final product；
The preparation of need testing solution takes Ginseng Leaf, crosses No. four sieves, and about 0.4g is accurately weighed, in putting conical flask with cover,
Precision adds 80% methyl alcohol 25ml, and close plug, weighed weight is put refluxing extraction 2.5 hours in 75 DEG C of water-baths, let cool, then weighed heavy
Amount, the weight of less loss is supplied with 80% methyl alcohol, is shaken up, and is filtered, and takes subsequent filtrate, is obtained final product；
Determination method is accurate respectively to draw reference substance solution and each 2 μ l of need testing solution, injects liquid chromatograph, determines, with
External standard method calculates content, obtains final product.
Embodiment 2
It is micro- warp of filler that chromatographic condition uses octadecylsilane chemically bonded silica with system suitability chromatographic column
Post；With acetonitrile as mobile phase A, water is Mobile phase B, carries out gradient elution, and gradient is：0~9 minute, mobile phase A was by 18%
Gradual change is to 20%；9~13 minutes, mobile phase A was by 20% gradual change to 28%；13~28 minutes, mobile phase A be 28% gradual change extremely
35%；Detection wavelength is 203nm；Flow velocity 0.4ml/min；
The preparation of mixed reference substance solution
Weigh ginsenoside Rg1Reference substance, ginsenoside Re's reference substance, ginsenoside Rb1Reference substance, ginsenoside Rb2It is right
It is appropriate according to product, 20 (S)-GF1 reference substances, ginsenoside Rd's reference substance, it is accurately weighed, plus methyl alcohol is made every 1ml containing people
Ginseng saponin(e Rg1100 μ g, the μ g of ginsenoside Re 500, ginsenoside Rb150 μ g, ginsenoside Rb280 μ g, 20 (S)-ginsenosides
The mixed solution of the μ g of F1 50, the μ g of ginsenoside Rd 200, obtains final product；
The preparation of need testing solution takes Ginseng Leaf, crosses No. four sieves, and about 0.4g is accurately weighed, in putting conical flask with cover,
Precision adds 60% methyl alcohol 25ml, and close plug, weighed weight is put refluxing extraction 3 hours in 82 DEG C of water-baths, let cool, then weighed weight,
The weight of less loss is supplied with 60% methyl alcohol, is shaken up, filtered, take subsequent filtrate, obtained final product；
Determination method is accurate respectively to draw reference substance solution and each 1 μ l of need testing solution, injects liquid chromatograph, determines, with
External standard method calculates content, obtains final product.
Embodiment 3
It is micro- warp of filler that chromatographic condition uses octadecylsilane chemically bonded silica with system suitability chromatographic column
Post；With acetonitrile as mobile phase A, water is Mobile phase B, carries out gradient elution, and gradient is：0~9 minute, mobile phase A was by 18%
Gradual change is to 20%；9~13 minutes, mobile phase A was by 20% gradual change to 28%；13~28 minutes, mobile phase A be 28% gradual change extremely
35%；Detection wavelength is 203nm；Flow velocity 0.4ml/min；
The preparation of mixed reference substance solution
Weigh ginsenoside Rg1Reference substance, ginsenoside Re's reference substance, ginsenoside Rb1Reference substance, ginsenoside Rb2It is right
It is appropriate according to product, 20 (S)-GF1 reference substances, ginsenoside Rd's reference substance, it is accurately weighed, plus methyl alcohol is made every 1ml containing people
Ginseng saponin(e Rg1100 μ g, the μ g of ginsenoside Re 500, ginsenoside Rb150 μ g, ginsenoside Rb280 μ g, 20 (S)-ginsenosides
The mixed solution of the μ g of F1 50, the μ g of ginsenoside Rd 200, obtains final product；
The preparation of need testing solution takes Ginseng Leaf, crosses No. four sieves, and about 0.4g is accurately weighed, in putting conical flask with cover,
Precision adds 80% methyl alcohol 25ml, and close plug, weighed weight is put refluxing extraction 3 hours in 75 DEG C of water-baths, let cool, then weighed weight,
The weight of less loss is supplied with 80% methyl alcohol, is shaken up, filtered, take subsequent filtrate, obtained final product；
Determination method is accurate respectively to draw reference substance solution and each 2 μ l of need testing solution, injects liquid chromatograph, determines, with
External standard method calculates content, obtains final product.
Above-described embodiment carries out Method validation according to pharmacopeia, result presentation method accurately and reliably, it is sensitive, exclusive, respectively
Item index meets the requirement of quality control.
Claims (4)
1. a kind of six kinds of content assaying methods of Ginsenosides in folium panacis japonici cum caule, six kinds of ginseng saponins compositions are ginseng soap
Glycosides Rg1, ginsenoside Re, ginsenoside Rb1, ginsenoside Rb2, 20 (S)-ginseng saponin Fs1And ginsenoside Rd；The method is adopted
With ultra-performance liquid chromatography, it is characterised in that the content assaying method is as follows：
Chromatographic condition and system suitability chromatographic column use octadecylsilane chemically bonded silica micro- through post for filler；
With acetonitrile as mobile phase A, water is Mobile phase B, carries out gradient elution, and gradient is：0~9 minute, mobile phase A by 18% gradually
Fade to 20%；9~13 minutes, mobile phase A was by 20% gradual change to 28%；13~28 minutes, mobile phase A be 28% gradual change extremely
35%；Detection wavelength is 203nm；Flow velocity 0.4ml/min；
The preparation of mixed reference substance solution
Weigh ginsenoside Rg1Reference substance, ginsenoside Re's reference substance, ginsenoside Rb1Reference substance, ginsenoside Rb2Control
Product, 20 (S)-GF1 reference substances, ginsenoside Rd's reference substance are appropriate, accurately weighed, plus methyl alcohol is made every 1ml containing ginseng
Saponin(e Rg1100 μ g, the μ g of ginsenoside Re 500, ginsenoside Rb150 μ g, ginsenoside Rb280 μ g, 20 (S)-GF1s
The mixed solution of 50 μ g, the μ g of ginsenoside Rd 200, obtains final product；
The preparation of need testing solution takes Ginseng Leaf, crosses No. four sieves, and about 0.4g is accurately weighed, in putting conical flask with cover, essence
60~80% methyl alcohol 25ml of close addition, close plug, weighed weight is put refluxing extraction 2.5~3 hours in 75~82 DEG C of water-baths, is let cool,
Weighed weight, the weight of less loss is supplied with same concentration methyl alcohol again, is shaken up, filtration, takes subsequent filtrate, is obtained final product；
Determination method is accurate respectively to draw reference substance solution and each 1~2 μ l of need testing solution, injects liquid chromatograph, determines, with
External standard method calculates content, obtains final product.
2. content assaying method according to claim 1, it is characterised in that the test liquid is heated to reflux the time in preparing
It is 2.5 hours.
3. content assaying method according to claim 1, it is characterised in that the concentration of methyl alcohol is in prepared by the test liquid
80%.
4. the content assaying method according to any one of claims 1 to 3, it is characterised in that the content assaying method is as follows：
Chromatographic condition and system suitability chromatographic column use octadecylsilane chemically bonded silica micro- through post for filler；
With acetonitrile as mobile phase A, water is Mobile phase B, carries out gradient elution, and gradient is：0~9 minute, mobile phase A by 18% gradually
Fade to 20%；9~13 minutes, mobile phase A was by 20% gradual change to 28%；13~28 minutes, mobile phase A be 28% gradual change extremely
35%；Detection wavelength is 203nm；Flow velocity 0.4ml/min；
The preparation of mixed reference substance solution
Weigh ginsenoside Rg1Reference substance, ginsenoside Re's reference substance, ginsenoside Rb1Reference substance, ginsenoside Rb2's control
Product, 20 (S)-GF1 reference substances, ginsenoside Rd's reference substance are appropriate, accurately weighed, plus methyl alcohol is made every 1ml containing ginseng
Saponin(e Rg1100 μ g, the μ g of ginsenoside Re 500, ginsenoside Rb150 μ g, ginsenoside Rb280 μ g, 20 (S)-GF1s
The mixed solution of 50 μ g, the μ g of ginsenoside Rd 200, obtains final product；
The preparation of need testing solution takes Ginseng Leaf, crosses No. four sieves, and about 0.4g is accurately weighed, in putting conical flask with cover, essence
80% methyl alcohol 25ml of close addition, close plug, weighed weight is put refluxing extraction 2.5 hours in 82 DEG C of water-baths, is let cool, then weighed weight,
The weight of less loss is supplied with 80% methyl alcohol, is shaken up, filtered, take subsequent filtrate, obtained final product；
Determination method is accurate respectively to draw reference substance solution and each 2 μ l of need testing solution, injects liquid chromatograph, determines, with external standard
Method calculates content, obtains final product.
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| CN107478753A (en) * | 2017-10-18 | 2017-12-15 | 中国农业科学院特产研究所 | A kind of assay method of general ginsenoside |
| CN107737144A (en) * | 2017-10-18 | 2018-02-27 | 中国农业科学院特产研究所 | The extracting method of ginsenoside in a kind of ginseng roots |
| CN107807179A (en) * | 2017-09-20 | 2018-03-16 | 北京北林先进生态环保技术研究院有限公司 | A kind of method that while qualitative, quantitative determines a variety of ginsenoside monomers |
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| CN107807179A (en) * | 2017-09-20 | 2018-03-16 | 北京北林先进生态环保技术研究院有限公司 | A kind of method that while qualitative, quantitative determines a variety of ginsenoside monomers |
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| CN107737144A (en) * | 2017-10-18 | 2018-02-27 | 中国农业科学院特产研究所 | The extracting method of ginsenoside in a kind of ginseng roots |
| CN109900818A (en) * | 2019-01-30 | 2019-06-18 | 广西壮族自治区食品药品检验所 | Gen-seng haulms mix pseudo- inspection method in 'Qipi ' |
| CN113433232A (en) * | 2021-06-10 | 2021-09-24 | 天津中医药大学 | Method for measuring ginsenoside content in ginseng traditional Chinese medicine |
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