CN106706592A - Raman spectrum-based pesticide residual detection system - Google Patents
Raman spectrum-based pesticide residual detection system Download PDFInfo
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- CN106706592A CN106706592A CN201510439317.XA CN201510439317A CN106706592A CN 106706592 A CN106706592 A CN 106706592A CN 201510439317 A CN201510439317 A CN 201510439317A CN 106706592 A CN106706592 A CN 106706592A
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Abstract
The invention discloses a Raman spectrum-based pesticide residual detection system. The system comprises: a preprocessing device used for processing an object to be detected to form a processed liquid to be detected; an optical detection device used for emitting excitation lights to the processed liquid to be detected and collecting Raman scattering lights from the processed liquid to be detected; and a data processing device used for extracting the Raman spectrum from the Raman scattering lights and determining whether the object to be detected contains pesticide residues or not based on the Raman spectrum, wherein the preprocessing device comprises a plurality of preprocessing chambers. The Raman spectrum-based pesticide residual detection system realizes highly-effective, simple and accurate detection of pesticide residues, and is especially suitable for onsite rapid detection.
Description
Technical field
Raman light is based on the present invention relates to the Detection Technologies of Pesticide Residues field, more particularly to one kind
The Detecting Pesticide system of spectrum.
Background technology
Agricultural chemicals is played an important role in the preventing and treating of diseases and pests of agronomic crop, and the use of agricultural chemicals is
Agricultural production brings huge interests.However, the unreasonable of agricultural chemicals uses, also bring tight
The environmental problem and food-safety problem of weight.Additionally, countries in the world have formulation file to specify
Related agricultural chemicals maximum residue limit, because standard is inconsistent, the annual agricultural byproducts agricultural chemicals of China
Residual does not meet international requirement and influences outlet, causes great direct economic loss.Cause
This carries out detection and has great significance to residues of pesticides.The analysis and inspection of current residues of pesticides
Survey depends on the large-scale instrument in laboratory to complete, and still lacks suitable for such as agriculture
The Detecting Pesticide equipment of the applications such as the rapid field detection of medicine residual.Accordingly, it would be desirable to one
Plant the accurate system of simple to operate, result and be applied to Detecting Pesticide.
The content of the invention
It is an object of the invention to provide a kind of Detecting Pesticide system based on Raman spectrum,
It can quickly, efficiently and accurately determine that the pesticide residue in food is (especially organic
Phosphorus agricultural chemicals) composition and concentration.
The embodiment provides a kind of Detecting Pesticide system based on Raman spectrum
System, including：
Pre-processing device, for determinand to be processed into determinand treatment fluid；
Optical detection apparatus, treat for launching exciting light and collecting to come to determinand treatment fluid
Survey the Raman diffused light of thing treatment fluid；With
Data processing equipment, for extracting Raman spectrum from the Raman diffused light and being based on
Whether the Raman spectrum determines contain pesticide residue in determinand, wherein,
The pre-processing device includes multiple pre-treatment rooms.
In one embodiment, the multiple pre-treatment room includes：
First pre-treatment room, is provided with treatment room before described first and smashs part to pieces, described
Part is smashed to pieces for carrying out determinand to smash homogenized to pieces；
Second pre-treatment room, extraction element is provided with treatment room before described second, is used for
Solution is extracted from the determinand by smashing to pieces to form determinand extract solution；
3rd pre-treatment room, scavenging material is provided with treatment room before the described 3rd, is used for
Filtering interfering material is forming determinand scavenging solution from determinand extract solution；With
4th pre-treatment room, concentration and solvent switch are provided with treatment room before the described 4th
Device, for carrying out concentration to determinand scavenging solution, and by solvent switch into ultra-pure water.
In one embodiment, the multiple pre-treatment room also includes：
5th pre-treatment room, in treatment room before the described 5th, will be by the 4th pre-treatment room
The determinand scavenging solution for the treatment of mixes to be formed at the determinand with Raman spectrum Contrast agent
Reason liquid.
In one embodiment, the pre-processing device also includes：
Raman spectrum Contrast agent preparation room, the Raman spectrum Contrast agent preparation room is set
Having heaters, agitator and centrifugal device, for preparing Raman spectrum Contrast agent, and enter
Row concentration.
In one embodiment, the described first to the 5th pre-treatment room present position height successively
Reduce.
In one embodiment, the adjacent preceding place of each two in the described first to the 5th pre-treatment room
Separated by displaceable dividing plate respectively between reason room, the displaceable dividing plate can be made by displacement
Determinand, determinand extract solution or determinand scavenging solution are moved to therewith from a pre-treatment room
Adjacent another pre-treatment room.
In one embodiment, the data processing equipment includes：
With reference to Raman spectrum stock's storage unit, the agricultural chemicals for storing principal component and concentration is residual
Stay the reference Raman spectrum of thing；
Raman spectrum data processing unit, for the Raman to being extracted in the Raman diffused light
Spectrum carries out spectrogram treatment, to improve the accuracy of mass spectrum database；
Pesticide residue composition detection unit, for the drawing extracted from the Raman diffused light
Extract characteristic peak in graceful spectrum, and by the peak position at this feature peak with reference to Raman spectrum compare with
Pesticide residue composition in detection determinand；With
Pesticide residue concentration detecting unit, for by the peak intensity at this feature peak with refer to Raman
Spectrum compares to detect the concentration of the pesticide residue in determinand.
In one embodiment, the agricultural chemicals includes organophosphorus pesticide.
In one embodiment, the Detecting Pesticide system also includes：
Housing, sets for accommodating the pre-processing device, optical detection apparatus and data processing
It is standby,
Wherein, the housing is provided with determinand input port and display screen, the determinand input
Mouth is for receiving determinand and being sent to the pre-processing device, the display screen and data
Processing equipment is connected, for showing Detecting Pesticide result.
In one embodiment, the data processing equipment also includes controller, for preceding place
The operation of reason equipment and optical detection apparatus coordinates control.
Above-mentioned technical proposal of the invention can provide a kind of residues of pesticides based on Raman spectrum
Detecting system.It can be realized for the agricultural chemicals in such as food (such as water fruits and vegetables etc.)
Efficient, the easy and accurate inspection of residual, is particularly suited for field quick detection.
Brief description of the drawings
Fig. 1 shows the structural representation of Detecting Pesticide system according to an embodiment of the invention
Figure；
Fig. 2 shows the schematic diagram of pre-processing device according to an embodiment of the invention；
Fig. 3 shows the schematic diagram of data processing equipment according to an embodiment of the invention；
Fig. 4 shows the agricultural chemicals of the phosmet containing various concentrations and does not contain the agricultural chemicals
The SERS curve map of apple；With
Fig. 5 schematically show the characteristic peak peak intensity of the reference Raman spectrum of phosmet with it is dense
The graph of a relation of degree.
Specific embodiment
Below by embodiment, and with reference to accompanying drawing, further is made to technical scheme
Specific description.In the description, same or analogous drawing reference numeral represents same or similar
Part.Following explanations referring to the drawings to embodiment of the present invention are intended to of the invention total
Body inventive concept is explained, and is not construed as to a kind of limitation of the invention.
Fig. 1 shows the structure of Detecting Pesticide system 100 according to an embodiment of the invention
Schematic diagram.The Detecting Pesticide system 100 includes：Pre-processing device 10, for that will treat
Survey thing and be processed into determinand treatment fluid；Optical detection apparatus 20, for determinand treatment fluid
Transmitting exciting light simultaneously collects the Raman diffused light from pretreatment solution；And data processing equipment
30, for extracting Raman spectrum from the Raman diffused light and being determined based on the Raman spectrum
Whether contain pesticide residue in determinand.
In practice, the detection object of determinand, i.e. residues of pesticides can include various food
(such as water fruits and vegetables, grain, nutriment), medicine and other may be because of residues of pesticides
And cause any article of harm, such as animal feeding-stuff etc..Pre-processing device 10 is for improving
The range of application meaning of accuracy of detection and expansion detecting system is very big, and it can be by determinand
Manage into determinand treatment fluid to be detected for optical detection apparatus 20, compared to directly to be measured
Thing carries out optics Raman detection, and detection is carried out to determinand processing solution has precision higher.
Pre-processing device 10 can include multiple pre-treatment rooms.Each pre-treatment room is for determinand meeting
Carry out different treatment.In this way, pre-processing device 10 is easy to be set with optical detection
It is integrated for 20 and data processing equipment 30, place before both having ensured to carry out determinand comprehensively
Reason can be readily transported again the Detecting Pesticide system 100 and take to improve accuracy of detection
Band, so as to be applied to field quick detection.
In one example, the Detecting Pesticide system 100 can include housing 50 (as schemed
Shown in 1), for accommodating at the pre-processing device 10, optical detection apparatus 20 and data
Reason equipment 30.That is, the Detecting Pesticide system 100 can be overall equipment,
Can be for example portable set or can mobile unit.This contributes to Detecting Pesticide system
100 realize Site Detection, without being necessarily arranged in laboratory.However, the present invention is not limited
In this, for example, pre-processing device 10, optical detection apparatus 20 and data processing equipment 30
Can also be discrete equipment.
In one example, housing 50 can be provided with determinand input port 51 and display screen 52.
The determinand input port 51 is used to receive determinand and is sent to the pre-processing device 10.
Display screen 52 is electrically connected with data processing equipment 30, for showing Detecting Pesticide result.
As an example, as shown in figure 1, data processing equipment 30 can also include controller 310,
The controller 310 can be carried out to the operation of pre-processing device 10 and optical detection apparatus 20
Coordinate control, so as to operation of the determinand before each in treatment room and carry out optics inspection to it
The operation of survey can be automatically performed successively.This way it is possible to realize from determinand input port 51
It is put into after determinand, just testing result can be seen on display screen 52 by certain hour,
Without being operated by hand a step by a step.
As an example, Detecting Pesticide system 100 can be with according to an embodiment of the invention
Residual for detecting organophosphorus pesticide.The organophosphorus pesticide can include isocarbophos, first
Mix phosphorus, carbophenothion, Fonofos, methidathion, Entex, Hostathion, phosmet,
Phosalone and parathion-methyl etc..
In one example, the multiple pre-treatment room can include：First pre-treatment room 11,
Second pre-treatment room 12, the 3rd pre-treatment room 13 and the 4th pre-treatment room 14.Described
Can be provided with one pre-treatment room 11 and smash part 111 to pieces, the part of smashing to pieces will be for that will treat
Survey thing and smashed into pieces relatively uniform slurry (or to smash homogenized to pieces).As showing
Example, this is smashed to pieces, and part can include such as cutting blade, to roll hammer etc. various solid for smashing to pieces
Body or viscid instrument.Extractant can be provided with treatment room 12 before described second,
For carrying out extraction process to form determinand extract solution to the determinand by smashing to pieces.As
Example, extraction element 121 is also provided with the second pre-treatment room 12, and (such as centrifugation is extracted
Device, gyrator, agitator etc.), for being extracted to carry to the determinand by smashing to pieces
High extracting efficiency.Process before the described 3rd and be provided with scavenging material in room 13, for from treating
Filtering interfering material is surveyed in thing extract solution forming determinand scavenging solution.The scavenging material can
With the adsorbate such as including activated carbon or it is capable of the other materials of filtering interfering material.Interfering material
For example including organic acid, pigment, colloid and cellulose etc..The spectrum of interfering material may be right
Raman spectrum produces influence, and the amount or removal interfering material for reducing interfering material can improve drawing
The signal to noise ratio of graceful spectral signal, so as to improve the accuracy of detection.Processed before the described 4th
Enrichment facility can be provided with room 14, for determinand scavenging solution is carried out concentration with
Concentration is improved, and by solvent switch into ultra-pure water, to reduce organic solvent Raman light in itself
The interference to agricultural chemicals spectrum to be measured is composed, pretreatment liquid is obtained.The concentration for example can be with profit
Realized with modes such as heating evaporations.
As an example, the multiple pre-treatment room can also include：5th pre-treatment room 15,
In treatment room 15 before the described 5th, the determinand treatment that will be processed by the 4th pre-treatment room
Liquid mixes to form the prepare liquid with Raman spectrum Contrast agent.Raman spectrum Contrast agent
Can be used for improving the signal intensity of Raman spectrum, to improve detection accuracy and sensitivity.
Raman spectrum Contrast agent can include nano material Contrast agent, such as metal nano material,
The metal is for example including any one in gold, silver and copper or combinations thereof.
In one example, pre-processing device 10 can also include：Raman spectrum Contrast agent system
Standby room 17, the Raman spectrum Contrast agent preparation room 17 can set having heaters, stirring
Device and centrifugation transposition, for preparing Raman spectrum Contrast agent, it is possible to carry out centrifugal concentrating.
In the examples described above, Raman spectrum can have been pre-saved in the 5th pre-treatment room 15 to increase
The solution of strong reagent, it is also possible to when preparation starts detection operation in Raman spectrum Contrast agent
Raman spectrum Contrast agent is prepared in preparation room 17.
Although above-mentioned each pre-treatment room is it is shown in fig. 2, but this does not imply that above-mentioned
Each pre-treatment room is all necessary.In practice can according to the need for design and detection come
Required pre-treatment room is therefrom selected to perform pre-treatment operation.
In one example, the present position height of the first to the 5th pre-treatment room is reduced successively,
As shown in Figure 2.This arrangement contributes to determinand to be flowed successively through from the first pre-treatment room
Second, third, the 4th and the 5th pre-treatment room.However, this arrangement is not must
Must.For example, the first to the 5th pre-treatment room can also be in the height being substantially the same, and
Using means of transportation, such as manipulator, conveyer belt etc., by determinand or its extract solution or
Scavenging solution is transmitted between treatment room before each.
In one example, the adjacent pre-treatment of each two in the described first to the 5th pre-treatment room
Can be separated by displaceable dividing plate 18 respectively between room, the displaceable dividing plate can be by moving
Position makes determinand, determinand extract solution or determinand scavenging solution are moved to from a pre-treatment room
Another pre-treatment room adjacent thereto.The treatment He of room 13 before the 3rd is illustrate only in fig. 2
Displaceable dividing plate 18 between 4th pre-treatment room 14.When net in the 3rd pre-treatment room 13
Changing after the completion of operation, (can be for example moved in Fig. 2 by will be moved on displaceable dividing plate 18
Position shown in dotted line) it is open to allow determinand net with displaceable dividing plate 18 formed below
Changing the 4th pre-treatment room 14 of liquid inflow carries out concentration and solvent switch treatment.Displaceable dividing plate 18
Can also move down up to form opening, such as the second pre-treatment room 12, if needed
The clear liquid on the solution upper strata being located at by extraction in the second pre-treatment room 12 is extracted, then may be used
With the displaceable dividing plate 18 that will be located between the second pre-treatment room 12 and the 3rd pre-treatment room 13
Move down to form opening, thus allow determinand extract solution flow into the 3rd pre-treatment room 13 in.
As an example, the movement of displaceable dividing plate 18, can be that translation can also be rotated.
In an embodiment of the present invention, determinand, determinand extract solution or determinand scavenging solution
Transmission between treatment room before each, is not limited to above-mentioned example mode.For example, can be with
Realized using various ways such as manipulator, conveyer belt, gas pumpings.As an example, each
Individual pre-treatment room special gateway can be set transmit determinand, determinand extract solution or
Determinand scavenging solution.
In an embodiment of the present invention, above-mentioned each pre-treatment room can be the room of closing,
Can be open room (such as top surface opening), can process chamber itself in the past locular wall it is straight
Receiving determinand, determinand extract solution, determinand scavenging solution or Raman spectrum Contrast agent are connect,
It is to be measured to accommodate and process container and device to be separately provided during room can also be processed before each
Thing, determinand extract solution, determinand scavenging solution or Raman spectrum Contrast agent.
In an embodiment of the present invention, the operation in above-mentioned each pre-treatment room can enter automatically
OK, it is also possible to manually carry out.
As an example, optical detection apparatus 20 can include single objective table, for example can be by
Determinand is sent to the loading in the pretreatment solution by being formed after the treatment of pre-processing device 10
Optical detection is carried out on platform.Alternatively, optical detection apparatus 20 directly can also launch light
(such as the 5th pre-treatment room 15) is carried out in certain the pre-treatment room in pre-processing device 10
Raman optical detection.The scheme of the latter can cause the knot of the Detecting Pesticide system 100
Structure is more compact.
In one example, data processing equipment 30 can include：With reference to Raman spectrum library storage
Unit 301, the reference Raman spectrum for storing the pesticide residue of principal component and concentration；
Pesticide residue composition detection unit 302, for the Raman extracted from the Raman diffused light
Characteristic peak is extracted in spectrum, and the peak position at this feature peak is compared to examine with reference to Raman spectrum
The pesticide residue composition surveyed in determinand；With pesticide residue concentration detecting unit 303, use
It is residual to detect the agricultural chemicals in determinand in the peak intensity at this feature peak and reference Raman spectrum are compared
Stay the concentration of thing.Examined in pesticide residue composition detection unit 302 and pesticide residue concentration
When surveying the execution operation of unit 303, it is required for calling with reference to Raman spectrum stock storage unit 301
In reference Raman spectrum compare.And pesticide residue concentration detecting unit 303 can be with
That calls pesticide residue composition detection unit 302 enters one for the extraction result of characteristic peak
Step calculates the peak intensity of characteristic peak.
As an example, the data processing equipment 30 can also be processed including Raman spectrum data
Unit 304, for carrying out spectrogram treatment to the Raman spectrum extracted in the Raman diffused light,
To improve the accuracy of mass spectrum database.The spectrogram is processed for example can be smoothed including spectrogram,
Remove baseline etc..Spectrogram treatment can be performed in pesticide residue composition detection unit 302
Carried out before operation.
As an example, can with reference to the reference Raman spectrum in Raman spectrum stock storage unit 301
With by the Raman spectrum containing known farm chemical ingredients and the reference sample of concentration is measured come
Obtain.Can obtain with reference to Raman spectrum and stored at the scene to Detecting Pesticide
To in reference to Raman spectrum stock storage unit 301, so, can to greatest extent ensure right
The uniformity and stabilization of the testing conditions in reference sample and the testing conditions for testing sample
Property.Alternatively, in order to shorten Site Detection time and flow, it is also possible to (such as exist in advance
In laboratory) according to being obtained to the measurement of reference sample with reference to Raman spectrum and stored
To in reference to Raman spectrum stock storage unit 301.Alternatively, it is also possible to using for example commercial
Obtainable standard Raman spectroscopy picture library is used as the reference Raman spectrum in the present invention.Above-mentioned ginseng
Examine Raman spectrum can according to detection the need for (species of such as agricultural chemicals) changed or mended
Fill.
In order to the concentration to pesticide residue is detected, with reference to Raman spectrum stock's storage unit
Needed in 301 comprising the reference Raman spectrum curve for being directed to different pesticide concentrations.And usual
Raman spectrum detection in, only substance classes are detected, without being detected to concentration,
Thus be accordingly used in the reference Raman spectrum of comparison not comprising the Raman spectrum for being directed to various concentrations
Curve.Moreover, it is contemplated that in Detecting Pesticide, pesticide residue is mixed in food
System (composition such as aliphatic acid, carbohydrate, organic acid and pigment, preservative etc. comprising food
Additive) in, and the Raman spectrum curve of testing sample is likely to be subject to different food
The influence of system.It is above-mentioned in order to remove influence of the different food systems for testing result
It is various containing the pesticide residue of principal component and concentration with reference to that can include in Raman spectrum
The reference Raman spectrum or the numerous food not comprising any pesticide residue of food system
The reference Raman spectrum of system.Using many of the pesticide residue containing principal component and concentration
The reference Raman spectrum of food system is planted, can be carried out directly with the testing result of testing sample
Comparison and calculating.And drawn according to the reference of the numerous food system not comprising pesticide residue
Graceful spectrum, it is also possible to derive different food systems for the effect of Raman spectrum according to this
Effect can also help be searched in the Raman spectrum of testing sample and determined and pesticide residue
Corresponding characteristic peak.
In order to preferably detect the concentration of pesticide residue, as an example, in residues of pesticides inspection
Can also be according to the reference Raman spectrum of the reference sample with different pesticide concentrations during survey
The peak intensity of the characteristic peak that curve is set up in pesticide residue concentration and Raman spectrum curve or peak face
Long-pending relation table, graph of a relation or functional relation.
For example, Fig. 4 gives the agricultural chemicals of the phosmet containing various concentrations and does not contain
The SERS curve map of the apple of the agricultural chemicals (blank), gives dense in figure
Degree is respectively the Raman light of the agricultural chemicals phosmet of 5ppm, 3ppm, 1ppm and 0.5ppm
Spectral curve.Wherein blank represents the spectrogram of the blank apple for not containing agricultural chemicals, it can be seen that
In the apple treatment fluid spectrogram containing agricultural chemicals, the feature of multiple agricultural chemicals phosmets is occurred in that
Peak, is such as located at 607,672,1015,1119 and 1258cm-1Etc. characteristic peak substantially,
Wherein it is located at 607cm-1Peak intensity it is maximum, be to be caused by C=O flexural vibrations, 672cm-1
It is P=S stretching vibrations, 710cm-1It is phenyl ring breathing vibration pattern, 1015cm-1It is P-O-C
Key chattering, 1191cm-1It is P-O-CH3On c h bond vibration, 1258cm-1Belong to
S-CH2C-N key stretching vibrations on-N.Simultaneously it can be seen that these characteristic peaks are in blank apple
Do not exist in the curve of fruit treatment fluid, so the above characteristic peak can be as phosmet
The judgment basis of residues of pesticides.From fig. 4, it can be seen that the feature peak intensity of agricultural chemicals phosmet
Degree dies down with the reduction of concentration, in concentration 0.5mg/L, still is able to observe substantially
Agricultural chemicals characteristic peak.According to food standard GB/T 2763-2014《Pesticide is maximum
Residue limits》Regulation, maximum residue limit of the phosmet in apple be 3mg/L, institute
The detection requirement of GB can be reached in this approach.
As can be seen from Fig. 4, the peak intensity of the characteristic peak in Raman spectrum curve is with concentration increase
And increase, and as pesticide concentration as little as 0.5ppm, characteristic peak is still observed that.
On the other hand, the peak position (position of the Raman frequency shift i.e. corresponding to characteristic peak) of characteristic peak and
Do not change with the concentration of agricultural chemicals.Thus, for example, frequency displacement can be chosen at for 607cm-1
The characteristic peak at place sets up the relation of peak intensity and pesticide concentration, as shown in Figure 5.Selection is located at
607cm-1Place's characteristic peak, with peak intensity as ordinate, phosmet pesticide concentration is abscissa
Standard curve is drawn, as shown in figure 5, the standard curve that the range of linearity is 0.5-5mg/L is obtained,
Functional relation is：Y=7123.ln (x)+10612, coefficient R 2=0.995, can see
Outlet sexual intercourse is good, can be used for the quantitative analysis of phosmet residues of pesticides in apple.
Although in Fig. 5 being the peak intensity and pesticide concentration that characteristic peak is set up according to linear relationship
Relation, but this is schematical, it would however also be possible to employ other modes set up peak intensity and agricultural chemicals
The relation of concentration, for example, can use the mode such as second order or higher order curve, parabola
It is fitted, it is also possible to the peak intensity and pesticide concentration of characteristic peak are set up with the mode of list
Relation, or set up the peak intensity of characteristic peak and the pass of pesticide concentration in the form of functional relation
System.
Only with frequency displacement as 607cm in Fig. 5-1To setting up the peak of characteristic peak as a example by the characteristic peak at place
The relation with pesticide concentration is explained by force, but this is only exemplary, for example, may be used also
To set up the relation of peak intensity and pesticide concentration from the characteristic peak at other peak positions in Fig. 4,
Or the relation of peak area and pesticide concentration can also be set up.The extraction of peak area is carried with peak intensity
Take compared to more complicated, but may be influenceed smaller by noise interferences.
Detecting Pesticide system is the live fast of residues of pesticides according to an embodiment of the invention
Speed detection provides a kind of approach.Be integrated in the system for various pre-treatment rooms by it, without
Need using large-scale laboratory equipment, it becomes possible to obtain accurate Detection results, Neng Gousui
When realize efficiently accurate Detecting Pesticide everywhere.
Although with reference to accompanying drawing, the present invention is described, the embodiment disclosed in accompanying drawing
Be intended to it is illustrative to the preferred embodiment for the present invention, and it is not intended that to this hair
A kind of bright limitation.
Although some embodiments of present general inventive concept have been shown and have illustrated, this area is general
Logical technical staff will be understood that, in principle and the situation of spirit without departing substantially from this present general inventive concept
Under, these embodiments can be made a change, the scope of the present invention with claim and they
Equivalent is limited.
Claims (10)
1. a kind of Detecting Pesticide system based on Raman spectrum, including：
Pre-processing device, for determinand to be processed into determinand treatment fluid；
Optical detection apparatus, treat for launching exciting light and collecting to come to determinand treatment fluid
Survey the Raman diffused light of thing treatment fluid；With
Data processing equipment, for extracting Raman spectrum from the Raman diffused light and being based on
Whether the Raman spectrum determines contain pesticide residue in determinand, wherein,
The pre-processing device includes multiple pre-treatment rooms.
2. Detecting Pesticide system according to claim 1, wherein, it is the multiple
Pre-treatment room includes：
First pre-treatment room, is provided with treatment room before described first and smashs part to pieces, described
Part is smashed to pieces for carrying out determinand to smash homogenized to pieces；
Second pre-treatment room, extraction element is provided with treatment room before described second, is used for
Solution is extracted from the determinand by smashing to pieces to form determinand extract solution；
3rd pre-treatment room, scavenging material is provided with treatment room before the described 3rd, is used for
Filtering interfering material is forming determinand scavenging solution from determinand extract solution；With
4th pre-treatment room, concentration and solvent switch are provided with treatment room before the described 4th
Device, for carrying out concentration to determinand scavenging solution, and by solvent switch into ultra-pure water.
3. Detecting Pesticide system according to claim 2, wherein, it is the multiple
Pre-treatment room also includes：
5th pre-treatment room, in treatment room before the described 5th, will be by the 4th pre-treatment room
The determinand scavenging solution for the treatment of mixes to be formed at the determinand with Raman spectrum Contrast agent
Reason liquid.
4. Detecting Pesticide system according to claim 3, wherein, it is described before place
Reason equipment also includes：
Raman spectrum Contrast agent preparation room, the Raman spectrum Contrast agent preparation room is set
Having heaters, agitator and centrifugal device, for preparing Raman spectrum Contrast agent, and enter
Row concentration.
5. Detecting Pesticide system according to claim 3, wherein, described first
Present position height to the 5th pre-treatment room is reduced successively.
6. Detecting Pesticide system according to claim 3, wherein, described first
Into the 5th pre-treatment room respectively by displaceable dividing plate point between the adjacent pre-treatment room of each two
Every the displaceable dividing plate can make determinand, determinand extract solution or to be measured by displacement
Thing scavenging solution is moved to another pre-treatment room adjacent thereto from a pre-treatment room.
7. the Detecting Pesticide system according to any one of claim 1-6, wherein,
The data processing equipment includes：
With reference to Raman spectrum stock's storage unit, the agricultural chemicals for storing principal component and concentration is residual
Stay the reference Raman spectrum of thing；
Raman spectrum data processing unit, for the Raman to being extracted in the Raman diffused light
Spectrum carries out spectrogram treatment, to improve the accuracy of mass spectrum database；
Pesticide residue composition detection unit, for the drawing extracted from the Raman diffused light
Extract characteristic peak in graceful spectrum, and by the peak position at this feature peak with reference to Raman spectrum compare with
Pesticide residue composition in detection determinand；With
Pesticide residue concentration detecting unit, for by the peak intensity at this feature peak with refer to Raman
Spectrum compares to detect the concentration of the pesticide residue in determinand.
8. the Detecting Pesticide system according to any one of claim 1-6, wherein,
The agricultural chemicals includes organophosphorus pesticide.
9. the Detecting Pesticide system according to any one of claim 1-6, also wraps
Include：
Housing, sets for accommodating the pre-processing device, optical detection apparatus and data processing
It is standby,
Wherein, the housing is provided with determinand input port and display screen, the determinand input
Mouth is for receiving determinand and being sent to the pre-processing device, the display screen and data
Processing equipment is connected, for showing Detecting Pesticide result.
10. the Detecting Pesticide system according to any one of claim 1-6, its
In, the data processing equipment also includes controller, for being examined to pre-processing device and optics
The operation of measurement equipment coordinates control.
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| CN201510439317.XA CN106706592B (en) | 2015-07-23 | 2015-07-23 | Pesticide residue detecting system based on Raman spectrum |
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| CN201510439317.XA CN106706592B (en) | 2015-07-23 | 2015-07-23 | Pesticide residue detecting system based on Raman spectrum |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107560916A (en) * | 2017-10-31 | 2018-01-09 | 黄家寅 | The automatic pretreating device of residues of pesticides |
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| CN114137122A (en) * | 2021-12-01 | 2022-03-04 | 青岛海关技术中心 | Method for rapidly identifying component content of full-sucrose syrup on customs inspection site |
| CN113866205A (en) * | 2021-12-06 | 2021-12-31 | 天津海关动植物与食品检测中心 | Chinese herbal medicine fluorescence spectrum heavy metal detection device and detection method |
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