CN106706580B - A kind of application of modified enhanced green fluorescent protein as aluminium ion detection probe - Google Patents
A kind of application of modified enhanced green fluorescent protein as aluminium ion detection probe Download PDFInfo
- Publication number
- CN106706580B CN106706580B CN201611065899.0A CN201611065899A CN106706580B CN 106706580 B CN106706580 B CN 106706580B CN 201611065899 A CN201611065899 A CN 201611065899A CN 106706580 B CN106706580 B CN 106706580B
- Authority
- CN
- China
- Prior art keywords
- liquid
- fluorescent protein
- green fluorescent
- aluminium ion
- enhanced green
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
Landscapes
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Immunology (AREA)
- Chemical & Material Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Optics & Photonics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Abstract
A kind of application the present invention relates to modified enhanced green fluorescent protein as aluminium ion detection probe, using green fluorescent protein as parent, in amino acid sequence, tyrosine is directed the modified enhanced green fluorescent protein sports levodopa.This method is easy to operate, and aluminium ion detection is limited up to μm olL‑1Grade, and selectivity is high, meets the detection needs of actual sample.
Description
Technical field
The present invention relates to aluminium ion detection fields, and in particular to a kind of modified enhanced green fluorescent protein is detected as aluminium ion
The application of probe.
Background technique
Aluminium is a kind of common metal, has been widely used in life.But due to food chain accumulation, abuse abuse
Etc. reasons, aluminium element easily by aluminium ion in the form of influence ecological environment, even into human body, cause cerebral nerve degenerate,
Memory loss etc..Therefore, have great importance to the Concentration Testing of aluminium ion in different environments.
Currently, with traditional Element detection method, such as atomic absorption method, atomic emissions method and inductivity coupled plasma mass spectrometry
The methods of compare, fluorescence probe detection have the advantages that quick, convenient, sensitivity is low etc..But the fluorescence probe reported at present is logical
It is all often to be obtained by organic fully synthetic means, preparation process is cumbersome, and has used a large amount of organic examinations in the synthesis process
Agent, heavy metal catalyst etc..In order to detect certain metal ion species, secondary pollution is but caused in the detection process, against green
Chemical principle.In addition, many detections carry out in the living body in biological medicine analysis, it is desirable that fluorescence probe has high biology
Compatibility, it is nontoxic, be easy to degrade.
Green fluorescent protein (green fluorescent protein, GFP), which is that one kind is naturally occurring, is green fluorescence
Albumen in jellyfish is made of 238 natural amino acids.After folding, fluorescence chromophore is wrapped in 11 well
Among beta sheet block, barreled structure is formed.Its fluorescence radiation is stablized, high-efficient, is a kind of efficient fluorescence probe.In addition,
Since albumen acquisition process is not related to the use of any harmful toxic matter, and its biocompatibility is high, is widely used in point
The research fields such as sub- label, drug screening, Antibody Fusion, biosensor.
Ayyadurai et al. (Bioconjugate chemistry, 2011,22 (4): 551-555.) is in tyrosine defect
Culture has obtained a kind of green fluorescent protein (GFPdopa) of unnatural amino acid insertion, Argine Monohydrochloride in type Escherichia coli
Tyrosine in sequence is substituted by levodopa.The research into research, but does not disclose it the property of GFPdopa
Applied to aluminum ions detection.
Summary of the invention
In view of the above-mentioned deficiencies in the prior art, it is an object of the present invention to provide a kind of modified enhanced green fluorescent protein as aluminium from
The application of sub- detection probe, this method is easy to operate, and aluminium ion detection is limited up to μm olL-1Grade, and selectivity is high, meets real
The detection of border sample needs.
Technical solution provided by the present invention are as follows:
A kind of application of modified enhanced green fluorescent protein as aluminium ion detection probe, the modified enhanced green fluorescent protein
Using green fluorescent protein as parent, in amino acid sequence, tyrosine, which is directed, sports levodopa.
In above-mentioned technical proposal, since levodopa is in water solution system, there is high compatibility to aluminium ion.Therefore
After the tyrosine in green fluorescent protein chromophore is mutated into levodopa, chromophore can carry out with free aluminium ion
Cooperation then generates the variation of fluorescence.
The green fluorescent protein parent amino acid sequence is in (Bioconjugate such as Ayyadurai
Chemistry, 2011,22 (4): 551-555.) it discloses in the article delivered.
The amino acid sequence of the green fluorescent protein such as SEQ ID NO:1.
The green fluorescent protein is a kind of tubbiness albumen with fluorescence chromophore, can in normal E. coli into
Row expression, maximum emission wavelength is between 450~550nm, and ultraviolet maximum absorption band is between 450~550nm.
The modified enhanced green fluorescent protein is expressed in tyrosine defective escherichia coli；The green fluorescence egg
White variant amino acid sum for 238 (they not including Histag label), relative molecular weight 25000~30000 dalton it
Between.
Preferably, the modified enhanced green fluorescent protein is distributed in prepare liquid and is examined after dialysing, being lyophilized
It surveys.
Preferably, the detection temperature is 4~60 DEG C, the reaction time, prepare liquid pH value was 5~9 in 1~60min.
Further preferably, the detection temperature is 15~40 DEG C, the reaction time in 1~10min, prepare liquid pH value is 6~
8。
Preferably, the prepare liquid is practical contaminated liquid or simulated cushioned liquid.
Preferably, the practical contaminated liquid is by aluminium ion contaminated soil leachate or by the thin of aluminium ion pollution
Born of the same parents' culture solution.
Preferably, the simulated cushioned liquid is phosphate buffer (PBS), 2-N- morpholine propane sulfonic acid buffer
(MES), 3-N- morpholine propane sulfonic acid buffer (MOPS), 4- hydroxyethyl piperazineethanesulfonic acid buffer (HEPES) or trihydroxy methyl
Aminomethane-hydrochloride buffer (Tris-HCl).Further preferably 3-N- morpholine propane sulfonic acid buffer (MOPS) or 4- hydroxyl
Ethyl piperazidine ethanesulfonic acid buffer (HEPES).
Preferably, additive amount of the modified enhanced green fluorescent protein in prepare liquid is 1~10 μm of olL-1。
Compared with the existing technology, the beneficial effects of the present invention are embodied in:
(1) the present invention provides a kind of completely new aluminium ion detection probe, preparation process green non-pollutions, and probe is water-soluble
Property it is high, good biocompatibility can be used for the detection that biological sample includes intracellular aluminium ion concentration；
(2) probe selectivity provided by the present invention is high, in Na+,K+,Ag+,Ca2+,Mg2+,Ba2+,Zn2+,Cd3+,V3+Deng
In the presence of different ions, exists to aluminium ion concentration and obvious response to.
Specific embodiment
Following application examples can make those skilled in the art that the present invention, but do not limit the invention in any way be more fully understood.
The expression of modified enhanced green fluorescent protein
Modified enhanced green fluorescent protein is oriented mutation using the means of residue specificity insertion unnatural amino acid.It will
Tyrosine defective escherichia coli containing green fluorescent protein plasmid DNA in minimal medium (Minimal medium) into
Row culture is expressed under IPTG induction then in the minimal medium containing levodopa.It will be big after complete expression
Enterobacteria cracking, and purified with nickel column to cracking supernatant, wherein the elution fraction for issuing yellow-green fluorescence is mesh
Mark albumen.Being lyophilized again after protein solution is dialysed and obtaining greenish yellow solid is modified enhanced green fluorescent protein (GFPdopa).
The detection of aluminium ion concentration is by the way that probe from after the mixing of different samples to be tested, is passed through sepectrophotofluorometer meter
It calculates fluorescence intensity change and obtains corresponding aluminium ion concentration.
Application examples 1
1,0.14g GFPdopa protein solid is weighed to be dissolved in 1L HEPES buffer solution (pH=7.4) as detection liquid
(5μmol·L-1)。
2, it takes appropriate aluminum nitrate and sodium nitrate to be dissolved in HEPES buffer solution (pH=7.4), is made into 10,20,40,80 μ
mol·L-1Al3+With 50 μm of olL-1Na+The simulating pollution liquid co-existed in.
3, by 100 μ L detection liquid respectively with 100 μ L HEPES buffer solutions (blank control) and 100 μ L simulating pollution liquid (to
Survey liquid) mixing shake up after, stand 5min, control detection temperature be 25 DEG C when, with fluorescence spectrophotometer range meter detect prepare liquid fluorescence
Al is calculated according to linear equation in Strength Changes3+Concentration.The result shows that the detection probe can detecte simulation dirt
Aluminium ion concentration in dye liquor, and not by Na+Existing interference.
Application examples 2
1,0.28g GFPdopa protein solid is weighed to be dissolved in 1L HEPES buffer solution (pH=7.0) as detection liquid
(10μmol·L-1)。
2, it takes appropriate aluminum nitrate and potassium nitrate to be dissolved in HEPES buffer solution (pH=7.0), is made into 10,20,40,80 μ
mol·L-1Al3+With 50 μm of olL-1K+The simulating pollution liquid co-existed in.
3, by 200 μ L detection liquid respectively with 200 μ L HEPES buffer solutions (blank control) and 200 μ L simulating pollution liquid (to
Survey liquid) mixing shake up after, stand 1min, control detection temperature be 25 DEG C when, with fluorescence spectrophotometer range meter detect prepare liquid fluorescence
Al is calculated according to linear equation in Strength Changes3+Concentration.The result shows that the detection probe can detecte simulation dirt
Aluminium ion concentration in dye liquor, and not by K+Existing interference.
Application examples 3
1,0.14g GFPdopa protein solid is weighed to be dissolved in 1L HEPES buffer solution (pH=8.0) as detection liquid
(5μmol·L-1)。
2, it takes appropriate aluminum nitrate and silver nitrate to be dissolved in HEPES buffer solution (pH=8.0), is made into 10,20,40,80 μ
mol·L-1Al3+With 50 μm of olL-1Ag+The simulating pollution liquid co-existed in.
3,50 μ L detection liquid is (to be measured with 50 μ L HEPES buffer solutions (blank control) and 50 μ L simulating pollution liquid respectively
Liquid) mixing shake up after, stand 5min, control detection temperature be 15 DEG C when, with fluorescence spectrophotometer range meter detection prepare liquid fluorescence it is strong
Degree variation, according to linear equation, is calculated Al3+Concentration.The result shows that the detection probe can detecte simulating pollution
Aluminium ion concentration in liquid, and not by Ag+Existing interference.
Application examples 4
1,0.084g GFPdopa protein solid is weighed to be dissolved in 1L Tris-HCl buffer solution (pH=6.0) as inspection
Survey liquid (3 μm of olL-1)。
2, it takes appropriate aluminum nitrate and calcium nitrate to be dissolved in Tris-HCl buffer solution (pH=6.0), is made into 10,20,40,80 μ
mol·L-1Al3+With 50 μm of olL-1Ca2+The simulating pollution liquid co-existed in.
3, by 100 μ L detection liquid respectively with 100 μ L Tris-HCl buffers (blank control) and 100 μ L simulating pollution liquid
After (prepare liquid) mixing shakes up, 3min is stood, it is glimmering with fluorescence spectrophotometer range meter detection prepare liquid when control detection temperature is 15 DEG C
Al is calculated according to linear equation in intensity variation3+Concentration.The result shows that the detection probe can detecte simulation
Aluminium ion concentration in contaminated liquid, and not by Ca2+Existing interference.
Application examples 5
1,0.14g GFPdopa protein solid is weighed to be dissolved in 1L Tris-HCl buffer solution (pH=6.5) as detection
Liquid (5 μm of olL-1)。
2, it takes appropriate aluminum nitrate and barium nitrate to be dissolved in Tris-HCl buffer solution (pH=6.5), is made into 10,20,40,80 μ
mol·L-1Al3+With 50 μm of olL-1Ba2+The simulating pollution liquid co-existed in.
3, by 200 μ L detection liquid respectively with 200 μ L Tris-HCl buffers (blank control) and 200 μ L simulating pollution liquid
After (prepare liquid) mixing shakes up, stands 10min and detect prepare liquid with fluorescence spectrophotometer range meter when control detection temperature is 25 DEG C
Al is calculated according to linear equation in fluorescence intensity change3+Concentration.The result shows that the detection probe can detecte mould
Aluminium ion concentration in quasi- contaminated liquid, and not by Ba2+Existing interference.
Application examples 6
1,0.28g GFPdopa protein solid is weighed to be dissolved in 1L Tris-HCl buffer solution (pH=6.0) as detection
Liquid (10 μm of olL-1)。
2, it takes appropriate aluminum nitrate and magnesium nitrate to be dissolved in Tris-HCl buffer solution (pH=6.0), is made into 10,20,40,80 μ
mol·L-1Al3+With 50 μm of olL-1Mg2+The simulating pollution liquid co-existed in.
3, by 500 μ L detection liquid respectively with 500 μ L Tris-HCl buffers (blank control) and 500 μ L simulating pollution liquid
After (prepare liquid) mixing shakes up, stands 10min and detect prepare liquid with fluorescence spectrophotometer range meter when control detection temperature is 37 DEG C
Al is calculated according to linear equation in fluorescence intensity change3+Concentration.The result shows that the detection probe can detecte mould
Aluminium ion concentration in quasi- contaminated liquid, and not by Mg2+Existing interference.
Application examples 7
1,0.028g GFPdopa protein solid is weighed to be dissolved in 1L MOPS buffer solution (pH=8.0) as detection liquid
(1μmol·L-1)。
2, it takes appropriate aluminum nitrate and zinc nitrate to be dissolved in MOPS buffer solution (pH=8.0), is made into 10,20,40,80 μm of ol
L-1Al3+With 50 μm of olL-1Zn2+The simulating pollution liquid co-existed in.
3,100 μ L detection liquid is (to be measured with 100 μ L MOPS buffers (blank control) and 100 μ L simulating pollution liquid respectively
Liquid) mixing shake up after, stand 10min, control detection temperature be 37 DEG C when, with fluorescence spectrophotometer range meter detection prepare liquid fluorescence it is strong
Degree variation, according to linear equation, is calculated Al3+Concentration.The result shows that the detection probe can detecte simulating pollution
Aluminium ion concentration in liquid, and not by Zn2+Existing interference.
Application examples 8
1,0.14g GFPdopa protein solid is weighed to be dissolved in 1L MOPS buffer solution (pH=7.0) as detection liquid (5
μmol·L-1)。
2, it takes appropriate aluminum nitrate and cobalt nitrate to be dissolved in MOPS buffer solution (pH=7.0), is made into 10,20,40,80 μm of ol
L-1Al3+With 50 μm of olL-1Co2+The simulating pollution liquid co-existed in.
3, by 50 μ L detection liquid respectively with 50 μ L MOPS buffers (blank control) and 50 μ L simulating pollution liquid (prepare liquid)
After mixing shakes up, 3min is stood, when control detection temperature is 37 DEG C, is become with fluorescence spectrophotometer range meter detection prepare liquid fluorescence intensity
Change, according to linear equation, Al is calculated3+Concentration.The result shows that the detection probe can detecte in simulating pollution liquid
Aluminium ion concentration, and not by Co2+Existing interference.
Application examples 9
1,0.28g GFPdopa protein solid is weighed to be dissolved in 1L MOPS buffer solution (pH=7.5) as detection liquid
(10μmol·L-1)。
2, it takes appropriate aluminum nitrate and nitric acid vanadium to be dissolved in MOPS buffer solution (pH=7.5), is made into 10,20,40,80 μm of ol
L-1Al3+With 50 μm of olL-1V3+The simulating pollution liquid co-existed in.
3, by 1000 μ L detection liquid respectively with 1000 μ L MOPS buffers (blank control) and 1000 μ L simulating pollution liquid
After (prepare liquid) mixing shakes up, stands 10min and detect prepare liquid with fluorescence spectrophotometer range meter when control detection temperature is 37 DEG C
Al is calculated according to linear equation in fluorescence intensity change3+Concentration.The result shows that the detection probe can detecte mould
Aluminium ion concentration in quasi- contaminated liquid, and not by V3+Existing interference.
Application examples 10
1,0.14g GFPdopa protein solid is weighed to be dissolved in 1L HEPES buffer solution (pH=7.4) as detection liquid
(5μmol·L-1)。
2, appropriate soil is taken, 10000rpm centrifuging and taking supernatant after being mixed with MOPS buffer solution, and appropriate nitric acid is added
Aluminium is made into 10,20,40,80 μm of olL-1Al3+Practical contaminated liquid.
3, by 100 μ L detection liquid respectively with 100 μ L HEPES buffer solutions (blank control) and the 100 practical contaminated liquids of μ L (to
Survey liquid) mixing shake up after, stand 5min, control detection temperature be 25 DEG C when, with fluorescence spectrophotometer range meter detect prepare liquid fluorescence
Al is calculated according to linear equation in Strength Changes3+Concentration.The result shows that the detection probe can detecte simulation dirt
Aluminium ion concentration in dye liquor, and not by the interference of other substances in soil extract.
Application examples 11
1, it weighs 0.084g GFPdopa protein solid and is dissolved in 1L HEPES buffer solution (pH=7.4) buffer solution and make
To detect liquid (3 μm of olL-1)。
2, appropriate aluminum nitrate is added in the DMEM culture medium for cultivating cell, is made into 10,20,40,80 μm of olL-1Al3 +Practical contaminated liquid.
3, by 2000 μ L detection liquid respectively with 2000 μ L HEPES buffer solutions (blank control) and the 2000 practical contaminated liquids of μ L
After (prepare liquid) mixing shakes up, 3min is stood, it is glimmering with fluorescence spectrophotometer range meter detection prepare liquid when control detection temperature is 37 DEG C
Al is calculated according to linear equation in intensity variation3+Concentration.The result shows that the detection probe can detecte simulation
Aluminium ion concentration in contaminated liquid, and not by the interference of other substances in cell culture fluid.
Application examples 12
1, will just express finish Escherichia coli centrifugation after, be resuspended in HEPES buffer solution (pH=7.4), make OD
(600nm) value is 0.1, as detection liquid.
2, it is taking appropriate aluminum nitrate to be dissolved in HEPES buffer solution (pH=7.4), is being made into 10,20,40,80 μm of olL-1Al3 +Simulating pollution liquid.
3, by 1000 μ L detection liquid respectively with 1000 μ L HEPES buffer solutions (blank control) and the 1000 practical contaminated liquids of μ L
After (prepare liquid) mixing shakes up, stands 10min and detect prepare liquid with fluorescence spectrophotometer range meter when control detection temperature is 37 DEG C
Al is calculated according to linear equation in fluorescence intensity change3+Concentration, while confocal fluorescent microscopic sight can also be used
Examine Escherichia coli change in fluorescence situation.The result shows that the detection probe can detecte aluminium ion concentration in bacterial body, Ke Yiyong
In internal (in vivo) aluminium ion concentration detection.
SEQUENCE LISTING
<110>Zhejiang University
<120>application of a kind of modified enhanced green fluorescent protein as aluminium ion detection probe
<130>
<160> 1
<170> PatentIn version 3.3
<210> 1
<211> 238
<212> PRT
<213>artificial sequence
<400> 1
Met Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val
1 5 10 15
Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Arg Gly Glu
20 25 30
Gly Glu Gly Asp Ala Thr Asn Gly Lys Ile Thr Leu Lys Leu Ile Cys
35 40 45
Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Cys
50 55 60
Gly Tyr Gly Val Gln Cys Phe Ala Arg Tyr Pro Asp His Met Lys Arg
65 70 75 80
His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu Arg
85 90 95
Thr Ile Ser Phe Lys Asp Asp Gly Thr Phe Lys Thr Arg Ala Glu Val
100 105 110
Lys Phe Glu Gly Asp Thr Ile Val Asn Arg Ile Lys Leu Lys Gly Ile
115 120 125
Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn
130 135 140
Phe Asn Ser His Asn Lys Tyr Ile Thr Ala Asp Lys Gln Lys Asn Gly
145 150 155 160
Ile Lys Ala Asn Phe Lys Ile Arg His Asn Val Glu Asp Gly Ser Val
165 170 175
Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly Pro
180 185 190
Arg Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Val Ile Leu
195 200 205
Glu Asp Pro Asn Glu Lys Arg Asp His Met Val Leu His Glu Phe Val
210 215 220
Thr Ala Ala Gly Ile Thr His Gly Met Asp Glu Leu Tyr Lys
225 230 235
Claims (5)
1. a kind of application of modified enhanced green fluorescent protein as aluminium ion detection probe, which is characterized in that the green fluorescence
Using green fluorescent protein as parent, in amino acid sequence, tyrosine is directed protein mutant sports levodopa；It is described
Modified enhanced green fluorescent protein is distributed in prepare liquid and is detected after dialysing, being lyophilized；The prepare liquid is practical pollution
Liquid or simulated cushioned liquid；Additive amount of the modified enhanced green fluorescent protein in prepare liquid is 1~10 μm of olL-1。
2. application of the modified enhanced green fluorescent protein according to claim 1 as aluminium ion detection probe, feature exist
In the temperature of the detection is 4~60 DEG C, and the reaction time, prepare liquid pH value was 5~9 in 1~60min.
3. application of the modified enhanced green fluorescent protein according to claim 2 as aluminium ion detection probe, feature exist
In the detection temperature is 15~40 DEG C, and the reaction time, prepare liquid pH value was 6~8 in 1~10min.
4. application of the modified enhanced green fluorescent protein according to claim 1 as aluminium ion detection probe, feature exist
In the practical contaminated liquid is by aluminium ion contaminated soil leachate or the cell culture fluid polluted by aluminium ion.
5. application of the modified enhanced green fluorescent protein according to claim 1 as aluminium ion detection probe, feature exist
In the simulated cushioned liquid is phosphate buffer, 2-N- morpholine propane sulfonic acid buffer, 3-N- morpholine propane sulfonic acid buffering
Liquid, 4- hydroxyethyl piperazineethanesulfonic acid buffer or tris-HCI buffer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611065899.0A CN106706580B (en) | 2016-11-28 | 2016-11-28 | A kind of application of modified enhanced green fluorescent protein as aluminium ion detection probe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611065899.0A CN106706580B (en) | 2016-11-28 | 2016-11-28 | A kind of application of modified enhanced green fluorescent protein as aluminium ion detection probe |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106706580A CN106706580A (en) | 2017-05-24 |
| CN106706580B true CN106706580B (en) | 2019-08-06 |
Family
ID=58933874
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611065899.0A Active CN106706580B (en) | 2016-11-28 | 2016-11-28 | A kind of application of modified enhanced green fluorescent protein as aluminium ion detection probe |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106706580B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112179876B (en) * | 2019-07-02 | 2023-06-02 | 南京工业大学 | Method for detecting levodopa and tyrosinase by forming fluorescent copolymer in situ |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20120110633A (en) * | 2011-03-30 | 2012-10-10 | 영남대학교 산학협력단 | Fluorescent protein containing l-3, 4-dihydroxyphenylalanine as a metal biosensor |
| CN102952181A (en) * | 2011-08-26 | 2013-03-06 | 南京大学 | Fluorescence sensor protein, plasmid or cell for detecting cuprous ions, DNA (deoxyribonucleic acid) encoding protein and preparation method |
| CN105585625A (en) * | 2014-10-31 | 2016-05-18 | 北京义翘神州生物技术有限公司 | Enhanced green fluorescence protein |
-
2016
- 2016-11-28 CN CN201611065899.0A patent/CN106706580B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20120110633A (en) * | 2011-03-30 | 2012-10-10 | 영남대학교 산학협력단 | Fluorescent protein containing l-3, 4-dihydroxyphenylalanine as a metal biosensor |
| CN102952181A (en) * | 2011-08-26 | 2013-03-06 | 南京大学 | Fluorescence sensor protein, plasmid or cell for detecting cuprous ions, DNA (deoxyribonucleic acid) encoding protein and preparation method |
| CN105585625A (en) * | 2014-10-31 | 2016-05-18 | 北京义翘神州生物技术有限公司 | Enhanced green fluorescence protein |
Non-Patent Citations (2)
| Title |
|---|
| 以绿色荧光蛋白为报告基因的环境砷离子生物检测体系的构建;胡春霞等;《环境与健康杂志》;20130731;第30卷(第7期);第634-637页 |
| 用左旋多巴-示差脉冲伏安法间接测定生物样品中的铝;章福平等;《高等学校化学学报》;20000831;第21卷(第8期);第1205-1207页 |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106706580A (en) | 2017-05-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN111004804B (en) | Aptamer and kit for detecting enrofloxacin and ciprofloxacin and application of aptamer and kit | |
| Huang et al. | Black phosphorus-Au filter paper-based three-dimensional SERS substrate for rapid detection of foodborne bacteria | |
| Longo-Sorbello et al. | Cytotoxicity and cell growth assays | |
| CN101892300B (en) | Klebsiella pneumoniae detection kit and use method thereof | |
| KR101656847B1 (en) | Method for detecting asbestos | |
| CN107607502B (en) | It is a kind of using multicolor fluorescence carbon dots simultaneously and the method for Visual retrieval Multiple Classes of Antibiotics and the fluorescence detection instruction card of Multiple Classes of Antibiotics | |
| CN106905418A (en) | A kind of histidine fluorescence probe and its preparation method and application | |
| Liu et al. | A polymyxin B–silver nanoparticle colloidal system and the application of lipopolysaccharide analysis | |
| CN106706580B (en) | A kind of application of modified enhanced green fluorescent protein as aluminium ion detection probe | |
| Johnston et al. | Single-molecule fluorescence-based approach reveals novel mechanistic insights into human small heat shock protein chaperone function | |
| Morck et al. | Hypertrophic cardiomyopathy mutations in the pliant and light chain-binding regions of the lever arm of human β-cardiac myosin have divergent effects on myosin function | |
| CN103389382A (en) | Signal-enhanced test strip biosensor for detecting histone methylation | |
| US20130302827A1 (en) | Annexin-based apoptosis markers | |
| You et al. | Bacterial cellulose loaded with silver nanoparticles as a flexible, stable and sensitive SERS-active substrate for detection of the shellfish toxin DTX-1 | |
| Huang et al. | Zwitterion-modified antifouling swab joint “Snake-Eye” for detection of Salmonella in colored foodstuffs | |
| Liu et al. | Identification of coexistence of biological and non-biological aerosol particles with DAPI (4′, 6-diamidino-2-phenylindole) stain | |
| CN106164292A (en) | Use SOMAmer by the method for microorganism in detection method based on fluorescence detection sample | |
| CN109187970B (en) | It is a kind of quickly to detect aquatic products disease gold mark nucleic acid test strip and preparation method thereof | |
| Sholar et al. | The Role of Viruses and Viral Lysis in Changing the Optical Properties of the Water Environment of their Habitat | |
| Juknius et al. | In-situ measurements of bacteria resistance to antimicrobial agents employing leaky mode sub-wavelength diffraction grating | |
| Gu et al. | Sensitive determination of proteins with naphthol green B by resonance light scattering technique | |
| CN103592167B (en) | Based on the Detection Methods of Fluoroquinolones Residue of luciferase mark engineering bacteria | |
| Wu et al. | Selenium-based nanozyme as a fluorescence-enhanced probe and imaging for chlortetracycline in living cells and foods | |
| CN202189009U (en) | High-sensitivity chromogenic TAL kit | |
| CN110272971A (en) | ATP bioluminescence lgCB-lgIBThe method that calibration curve method detects antibiotic paint bacteria resistance energy |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |