CN106701568B - Optical biological cell culture device - Google Patents
Optical biological cell culture device Download PDFInfo
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- CN106701568B CN106701568B CN201510442947.2A CN201510442947A CN106701568B CN 106701568 B CN106701568 B CN 106701568B CN 201510442947 A CN201510442947 A CN 201510442947A CN 106701568 B CN106701568 B CN 106701568B
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Abstract
The invention discloses an optical biological cell culture device, relates to the technical field of optical biological cell culture, and can solve the technical problem of low light utilization rate. The optical biological cell culture device comprises a support, wherein at least two culture surfaces arranged at intervals are hung on the support, and the non-hanging end of each culture surface is connected with a regulating device for regulating the direction of the non-hanging end. The photo-biological cell culture device is used for culturing photo-biological cells.
Description
Technical Field
The invention relates to the technical field of optical biological cell culture, in particular to an optical biological cell culture device.
Background
Microalgae is a photo-biological cell widely researched and applied, is a biological resource with abundant resources, various varieties, high growth speed and great application value, can be applied to the fields of food, medicine, genetic engineering, liquid fuel and the like, and more enterprises begin to culture microalgae on a large scale based on the application value of microalgae.
Generally, microalgae culture is mainly performed in a "water body culture" mode, and a microalgae culture device adopted in the culture mode comprises an open raceway pond or a closed/semi-closed reactor. The open raceway pond can only enable microalgae cells on the surface layer of the liquid level of the nutrient solution to carry out photosynthesis, so that the yield of microalgae is low; because the closed/semi-closed reactor is higher in height, the pressure of the nutrient solution on the bottom and the middle part of the closed/semi-closed reactor is higher, so that the manufacturing cost and the energy consumption of gas supply are higher. In order to overcome the disadvantages of the above-mentioned "water body culture" method, the "attached culture" method of microalgae should be used, and the "attached culture" method of microalgae refers to that microalgae cells are fixed or attached on the surface or/and inside of the culture medium to culture the microalgae. For example, chinese patent publication No. CN103289888A discloses a plate-inserted microalgae semi-dry solid-state adherent culture device, in which a plurality of culture plates are fixed on the ground, microalgae cells are fixed on the culture plates, and a liquid distribution tube is used to supplement the culture liquid to maintain the efficient growth of the microalgae cells on the culture plates.
In the microalgae cultivating apparatus disclosed in the above patent document, the solar altitude is constantly changed during the cultivation of microalgae, so that the light irradiated to the light receiving surface of the cultivating plate is also changed. Around noon, the solar altitude angle is great, and will have sunshine direct irradiation ground between the adjacent culture plate, causes the loss in a large number of light energy, and the light utilization ratio is lower.
Disclosure of Invention
The invention provides an optical biological cell culture device which can solve the technical problem of low light utilization rate.
In order to achieve the purpose, the invention adopts the following technical scheme:
the utility model provides a light biological cell culture device, includes the support, hang the culture face that has two at least intervals to set up on the support, the non-end that hangs of culture face is connected with and is used for adjusting the adjusting device of the position of non-end that hangs.
In the process of culturing the photobiological cells, the photobiological cells are attached to the culture surface, and sunlight irradiates the light receiving surface of the culture surface to provide light energy required by the growth of the photobiological cells; in a day, the light energy received by the light receiving surface and the light receiving surface of the culture surface is continuously changed along with the continuous change of the altitude angle and the azimuth angle of the sun, when the sunlight irradiates on the ground between the adjacent culture surfaces to cause light energy waste, the direction of the non-suspension end of the culture surface is adjusted through the adjusting device, namely the vertical direction displacement and/or the horizontal direction displacement of the culture surface is adjusted, so that the non-suspension end of the culture surface swings around the suspension end of the culture surface, the light receiving surface of the culture surface adapts to the change of the altitude angle and the azimuth angle of the sun, the culture surface blocks the sunlight irradiating on the ground between the adjacent culture surfaces, the illumination area on the ground between the adjacent culture surfaces is reduced, the light energy received by the light receiving surface of the culture surface is increased, the light energy loss between the adjacent culture surfaces is reduced.
Drawings
FIG. 1 is a perspective view of an optical biological cell culture apparatus according to an embodiment of the present invention;
FIG. 2 is a perspective view of the photo-biological cell culture apparatus shown in FIG. 1 with the culture surface adjusted;
FIG. 3 is a schematic view of the connection between the longitudinal support bar and the regulating device in the optical biological cell culture device shown in FIG. 1;
FIG. 4 is a schematic view of a cord length adjustment disk in an optical biological cell culture apparatus according to an embodiment of the invention;
FIG. 5 is a schematic view of another cord length adjustment disk in the optical biological cell culture apparatus according to the embodiment of the invention;
FIG. 6 is a schematic view of the connection between the liquid distributor and the culture surface of the optical biological cell culture apparatus according to the embodiment of the invention;
FIG. 7 is a schematic view of a culture section provided with an upper suspension member;
FIG. 8 is a schematic view showing the case where the light utilization efficiency and the light splitting efficiency of the culture section are in the optimum state when the solar altitude is determined in the example of the present invention;
FIG. 9 is a schematic view showing the case where the ground between the adjacent culture sections is illuminated when the elevation angle of the sun is determined;
FIG. 10 is a schematic view showing the light reception of the culture section after the adjustment of the culture section in FIG. 9;
FIG. 11 is a schematic view showing the case where the ground between the adjacent culture sections is illuminated when the solar azimuth is determined;
FIG. 12 is a schematic view showing the light reception of the culture section after the adjustment of the culture section in FIG. 11.
1-longitudinal support rod, 2-transverse support rod, 3-culture surface, 4-adjusting rope, 5-liquid collector, 6-liquid supplementing device, 7-rope length adjusting disc, 8-liquid distributor, 9-upper suspension member and 10-lower suspension member; 701-positioning section.
Detailed Description
For the convenience of understanding, the photo-biological cell culture device of the present invention will be described in detail below with reference to the accompanying drawings.
The present embodiment provides an optical biological cell culture apparatus, as shown in fig. 1 and fig. 2, the optical biological cell culture apparatus includes a support, at least two culture surfaces 3 arranged at intervals are suspended on the support, and an adjusting device for adjusting the orientation of the non-suspended end is connected to the non-suspended end of the culture surface 3. The optical biological cell culture device can be used for culturing microalgae.
It should be noted that the end of the culture section 3 suspended on the support is a suspension end, and the suspension end may be a point, two points, multiple points, or an edge, which is not limited in the embodiment of the present invention.
The non-suspended end of each culture section 3 may be connected to one or two adjustment devices, and when the non-suspended end of a culture section 3 is connected to two adjustment devices, the adjustment of the non-suspended end of the culture section 3 by the two adjustment devices may not be synchronized, e.g. the two adjustment devices may displace the non-suspended end of the culture section 3 differently in the vertical direction and/or in the horizontal direction, respectively.
The culture section 3 may be a rigid section or a flexible section. However, when the culture section 3 is a flexible section, the orientation of the non-suspended end of the culture section 3 is adjusted in a more number of ways, and therefore, in the embodiment of the present invention, it is preferable that the culture section 3 is a flexible section. In this case, the top end of the culture section 3 is a hanging end, and the bottom end of the culture section 3 is a non-hanging end.
In this embodiment, in the process of culturing photo-biological cells, the photo-biological cells are attached to the culture surface 3, and the sunlight irradiates the light receiving surface of the culture surface 3 and provides the light energy required for the growth of the photo-biological cells; the light energy received by the light receiving surface and the light receiving surface of the culture section 3 varies with the elevation and azimuth of the sun during the day. The following embodiments of the present invention describe how the optical biological cell culture apparatus adjusts the light energy received by the light receiving surface and the light receiving surface of the culture surface 3:
when sunlight irradiates on the ground between the adjacent culture surfaces 3 to cause light energy waste, the direction of the non-suspension end of the culture surface 3 is adjusted through the adjusting device, namely the vertical direction displacement and/or the horizontal direction displacement of the non-suspension end of the culture surface 3 are adjusted, so that the non-suspension end of the culture surface 3 swings around the suspension end of the culture surface, the light receiving surface of the culture surface 3 adapts to the change of the solar altitude angle and the solar azimuth angle, the culture surface 3 blocks the sunlight irradiating on the ground between the adjacent culture surfaces 3, the illumination area on the ground between the adjacent culture surfaces 3 is reduced, the light energy received by the light receiving surface of the culture surface 3 is increased, the light energy loss between the adjacent culture surfaces 3 is reduced, and the light utilization rate is improved. When the culture surfaces 3 just completely shield the sunlight on the ground between the adjacent culture surfaces 3, the light energy loss between the culture surfaces 3 is less, and the light utilization rate is higher.
In addition, in the above-mentioned adjustment of the orientation of the non-suspended end of the culture section 3, the culture section 3 can block the sunlight irradiated on the ground between the adjacent culture sections 3, and the sunlight is mainly irradiated on the lower half portion of the culture section 3, so that the light receiving amount of the lower half portion of the culture section 3 is increased, and the uniformity of the light receiving amount of the upper and lower half portions of the culture section 3 is improved.
Under the irradiation of sunlight, when two adjacent culture surfaces 3 generate shadows and are unevenly illuminated, the non-suspension end of each culture surface 3 is driven to move through the adjusting device, so that the non-suspension end of each culture surface 3 swings around the suspension end of the non-suspension end, the area of the shadow generated when the sunlight is irradiated on the adjacent culture surfaces 3 is reduced, the sunlight irradiated on the culture surfaces 3 is increased, the uniformity of the illumination of the culture surfaces 3 is improved, and the light splitting rate is improved; when the whole light receiving surface of the culture surface 3 can be irradiated by sunlight, the shadow on the adjacent culture surface 3 completely disappears, so that the light receiving of the culture surface 3 is more uniform, and the light splitting rate is higher.
In the above embodiment, the optical biological cell culture apparatus effectively improves the light utilization rate and the light splitting rate of the culture surface 3, so that the optical biological cells on the culture surface 3 obtain more and more uniform light energy, and the optical biological cells grow in an adequate and suitable light energy environment. On the premise of ensuring sufficient light energy, the specific support structure can be various. Illustratively, the support may comprise at least two longitudinal support bars, each arranged at a distance from the other, connected to the suspended end of the culture section 3, so as to suspend or support the culture section 3 in the air (i.e. at a certain height from the ground). For example, each two longitudinal support rods support one culture section 3, and specifically, both longitudinal support rods are connected to the hanging end of one culture section 3. So set up will make the quantity of bracing piece more, the structure of support is complicated, is unfavorable for practical application.
In order to solve the above problems, the support provided in the embodiment of the present invention may further include a transverse support bar, the longitudinal support bar is connected to the transverse support bar in a crossing manner to suspend or support the transverse support bar in the air, and the transverse support bar is connected to the suspension ends of the culture surfaces 3. In the preferred embodiment of the present invention, as shown in fig. 1 and fig. 2, the support comprises four vertically disposed longitudinal support rods 1, wherein the top ends of two longitudinal support rods 1 are connected to one transverse support rod 2, the top ends of the other two longitudinal support rods 1 are connected to the other transverse support rod 2, preferably, two transverse support rods 2 are both connected to the suspended end of each culture surface 3, and the non-suspended end of each culture surface 3 is connected to an adjusting device, for example, the top end of each culture surface 3 is designed to be a suspended end, and the bottom end of each culture surface 3 is designed to be a non-suspended end. Therefore, only six support rods are needed and are correspondingly connected, wherein four support rods are vertically arranged, and the other two support rods are transversely arranged, so that the hanging support function of the culture surface 3 can be realized. In the above-mentioned support structure, the structural shape of the horizontal support rod 2 can be column, tubular, rope-like and strip-like structures, etc., the longitudinal support rod 1 and the horizontal support rod 2 can both adopt common materials and structural shapes, and the support structure has the advantages of simple structure, easy processing and easy operation, thereby simplifying the structure of the optical biological cell culture device.
It should be noted that the bracket with the above structure also needs to consider the following problems during the use process: for example, when the culture section 3 is a flexible plane, the non-suspended end of the culture section 3 is connected to two adjusting devices, and the two adjusting devices adjust the non-suspended end of the culture section 3 asynchronously, because the two transverse support rods 2 connected to the suspended end of the culture section 3 are displaced relatively along the extending direction thereof, the culture section 3 can rotate along with the two transverse support rods, so that the distance between the two transverse support rods 2 of the culture section 3 needs to be increased, at this time, the culture section can be realized by elastically connecting the suspended end of the culture section 3 and the transverse support rods 2 (for example, connecting the suspended end of the culture section 3 and the transverse support rods 2 with an elastic material such as a spring), by using an auxiliary member (for example, connecting one end of a long auxiliary member with the suspended end of the culture section 3, and connecting the other end of the auxiliary member with the transverse support rods 2), and the like. For example, when the culture section 3 is a rigid surface, it can be realized by using an auxiliary member.
After the culture surface 3 is hung on the transverse supporting rod 2 of the bracket, the vertical displacement and/or the horizontal displacement of the non-hanging end of the culture surface 3 are adjusted according to the change rule of the solar altitude angle in one day, namely, the direction of the non-hanging end of the culture surface 3 is adjusted. As shown in FIG. 8, when there is no light on the ground between the adjacent culture section 3 and there is no shadow on the adjacent culture section 3, there is no need to adjust the orientation of the non-suspended end of the culture section 3; otherwise, the orientation of the non-suspended end of the culture section 3 is adjusted by the adjusting device so that the light-receiving surface of the culture section 3 is adapted to the change of the solar altitude, as described below.
Around noon, the sun's altitude is large, and sunlight is irradiated on the ground between the adjacent culture sections 3, and as shown in fig. 9, in order to avoid wasting of light energy when sunlight is irradiated on the ground between the adjacent culture sections 3, the non-suspended end of the culture section 3 is moved to the front upper side of the light receiving surface of the culture section 3, and in this adjustment process, the light irradiated area on the ground between the adjacent culture sections 3 is reduced, and the light energy received by the light receiving surface of the culture section 3 is increased until no sunlight is irradiated on the ground between the adjacent culture sections 3. Specifically, the "movement of the non-suspended end of the culture section 3 to the front upper side of the light-receiving surface of the culture section 3" may include the following three cases: in the first case, the horizontal displacement of the non-suspended end of the culture surface 3 is increased, no light is irradiated on the ground between two adjacent culture surfaces 3, and the light is irradiated on a certain part of the culture surface 3 and is not distributed on the whole culture surface 3, so that the light can be fully utilized, but the light splitting efficiency is low; in the second situation, the vertical displacement of the non-suspended end of the culture surface 3 is increased, light still irradiates the ground between two adjacent culture surfaces 3, but the light irradiating the culture surfaces 3 is distributed on the whole culture surface 3, so that the light splitting efficiency is high, but the light waste cannot be completely avoided; in the third case, as shown in fig. 10, both the horizontal displacement and the vertical displacement of the non-suspended end of the culture surface 3 are increased, no light is irradiated on the ground between two adjacent culture surfaces 3, and all light is incident on the culture surface 3, so that the light splitting efficiency is high, and the light energy waste is avoided.
When the sun is positioned right above the culture surface 3, the solar altitude 3 reaches the maximum value, the non-suspended end of the culture surface 3 is moved to the front of or the front upper part of the light receiving surface by the adjusting device until the non-suspended end of the culture surface 3 is connected with the culture surface 3 in front of the light receiving surface, the horizontal displacement of the non-suspended end of the culture surface 3 is just equal to the distance between the adjacent culture surfaces 3, and then the vertical displacement h of the non-suspended end of the culture surface 3 satisfies the following relational expression:
h≥H-(H2-a2)1/2；
wherein H is the vertical displacement of the non-suspended end of the culture section 3, H is the height of the culture section 3, and a is the distance between adjacent culture sections 3 when the culture section 3 is naturally suspended in a vertical state. When the above formula is satisfied with a mark of equal size, the culture section 3 is flat, and when the above formula is satisfied with a mark of greater size, the culture section 3 is curved. In addition, when the non-suspended end of the culture section 3 is connected to the two adjusting devices and the two adjusting devices are not used for adjusting the non-suspended end of the culture section 3 synchronously, although the vertical displacement generated by the adjustment of the non-suspended end of the culture section 3 by the two adjusting devices satisfies the above relation, the vertical displacement and/or the horizontal displacement generated by the two adjusting devices are different, and at this time, the culture section 3 may also be curved.
In the morning or evening, the sun altitude is small, and in order to avoid the occurrence of a shadow when sunlight is irradiated onto the adjacent culture section 3, the non-suspended end of the culture section 3 is moved to the rear lower side of the light-receiving surface of the culture section 3, preferably until no shadow is generated on the adjacent culture section 3, so that the light can be received on the entire light-receiving surface of the culture section 3, and the light can be received on the entire light-receiving surface of the culture section 3 more uniformly, thereby improving the light-splitting efficiency.
From the above analysis, it can be known that the culture surface 3 can swing around the suspending end by only adjusting the orientation of the non-suspending end of the culture surface 3, so that the light receiving surface of the culture surface 3 can adapt to the change of the solar altitude angle, thereby effectively improving the light utilization rate and the light splitting rate.
In the above embodiment, the orientation of the non-suspended end of the culture surface 3 is adjusted by the adjusting device along with the change of the solar altitude, so that the non-suspended end of the culture surface 3 swings around the suspended end, thereby improving the light utilization rate and the light splitting degree. However, not only the solar altitude angle is changed continuously but also the solar azimuth angle is changed continuously during a day, so as to avoid the loss of light energy caused by the illumination of the ground between the adjacent culture surfaces 3 due to the change of the solar azimuth angle, the embodiment of the invention also provides the following adjusting modes:
in the first adjustment mode, the hanging end of the culture surface 3 is disposed at one of the vertical side portions of the culture surface 3, the other vertical side portion is a non-hanging end, and the corresponding horizontal support rod 2 is an adjustable member, i.e., the adjustment device has the same function as the horizontal support rod 2, as shown in fig. 11 and 12, when there is illumination on the ground between the adjacent culture surfaces 3 due to the change of the solar azimuth angle, the adjustment device drives the non-hanging end of the culture surface 3 to move, so that the culture surface 3 swings around the hanging end thereof, and the light receiving surface of the culture surface 3 adapts to the change of the solar azimuth angle, thereby reducing the illumination area between the adjacent culture surfaces 3, and improving the light utilization rate. In the second adjustment mode, the top end of the culture surface 3 is a hanging end, the bottom end of the culture surface 3 is a non-hanging end, the hanging end of the culture surface 3 is connected with the two transverse support rods 2, and the non-hanging end of the culture surface 3 is connected with the adjustment device. The first adjustment method is applicable only when the culture section 3 is a rigid section, and the second adjustment method is applicable when the culture section 3 is a rigid section or a flexible section.
When the culture surface 3 is a flexible surface, the culture surface 3 may be made of a flexible material, and may be a textile, a polymer porous material product, a paper product, a felt product, or an algal cell aggregate, for example, natural fibers such as plant fibers, animal fibers, and mineral fibers, or chemical fibers such as regenerated fibers, synthetic fibers, and inorganic fibers, or natural materials such as metal, wood, and bamboo, or chemical materials such as plastic, silica gel, modified fibers, and ultrafine fibers; the culture section 3 may also be made of one or more materials.
When the orientation of the non-suspended end of the culture surface 3 is adjusted and the culture surface 3 is a flexible surface, the culture surface 3 is easy to deform, and the adjusting device can enable two ends of the non-suspended end of each culture surface 3 to move asynchronously, so that the culture surfaces 3 are twisted to receive sunlight irradiated between the adjacent culture surfaces 3 on the ground, and the culture surfaces 3 receive more sunlight, thereby improving the light utilization rate; also, it is possible to avoid a shadow generated when the adjacent culture sections 3 are shielded, thereby improving the light-splitting efficiency.
Because the solar altitude and the solar azimuth are changed simultaneously in one day, the sunlight irradiated to the ground between the adjacent culture surfaces 3 is often irradiated, wherein one part of the sunlight is irradiated through the top side edge of the culture surface 3, and the other part of the sunlight is irradiated through the vertical side edge of the culture surface 3; the sunlight irradiates the shade formed by the adjacent culture surface 3, wherein one part is formed by shielding the side edge at the top end of the culture surface 3, and the other part is formed by shielding the vertical side edge of the culture surface 3; in order to make the adjusting device more effectively adjust the orientation of the non-suspended end of the culture section 3, so as to be more favorable for improving the light utilization rate and the light splitting rate, the above adjusting mode for the culture section 3 can be comprehensively adopted. In the application process, a person skilled in the art can select an adjustment mode according to specific situations and requirements, and if the adjustment mode is as simple as possible and the adjustment times are as few as possible while the minimum light energy loss is realized, a mode of synchronously adjusting the vertical displacement and/or the horizontal displacement of the two ends of the non-suspension end of the culture surface 3 can be selected.
In addition, in order to improve the shape controllability of the culture section 3, an upper hanging member 9 is connected to the hanging end of the culture section 3 for supporting the hanging end of the culture section 3, and as shown in FIG. 7, both ends of the upper hanging member 9 are connected to two lateral support rods 1; the non-suspension end of the culture section 3 is connected with a lower suspension member 10 for supporting the non-suspension end of the culture section 3, and both ends of the lower suspension member 10 are connected with an adjusting device. Illustratively, the upper and lower suspension members 9 and 10 may be spindles. When the culture surface 3 is a flexible surface, the culture surface 3 is easy to deform, when the orientation of the non-suspension end of the culture surface 3 is adjusted, the shape of the culture surface 3 is not easy to control, however, after the upper suspension member 9 and the lower suspension member 10 are connected with the culture surface 3, the suspension end and the non-suspension end of the culture surface 3 can keep a straight state, the connection between the upper suspension member 9 and the transverse support rod 2 and the connection between the lower suspension member 10 and the adjusting device are facilitated, and the shape of the culture surface 3 is easier to control in the adjusting process.
In order to further simplify the structure of the photo-biological cell culture device, as shown in fig. 1-3, the adjusting device comprises two adjusting ropes 4, the two adjusting ropes 4 are connected with the non-hanging end of each culture surface 3, and after the orientation of the non-hanging end of each culture surface 3 is adjusted, the adjusting ropes 4 are tied to the corresponding positions of the longitudinal support rods 1 or the adjusting ropes 4 are tied to other fixtures. Wherein, adjusting device can also adopt other multiple structural shapes, for example, column, tubulose, strip etc. have the advantage of drawing materials easily, simple structure, and then simplified whole light biological cell culture device's structure.
It should be noted that, when one end of the adjusting rope 4 is connected to one of the longitudinal support rods 1, the other end is connected to the other longitudinal support rod 1, and the orientation of the non-suspended end of the culture surface 3 is adjusted, both ends of the adjusting rope 4 need to be adjusted simultaneously; when one end of the adjusting rope 4 is connected to one of the longitudinal support rods 1, the other end is connected to the culture section 3 located at the farthest position relative to the longitudinal support rod 1, and the orientation of the non-suspended end of the culture section 3 is adjusted, it is only necessary to adjust the end of the adjusting rope 4 connected to the longitudinal support rod 1. When the orientation of the non-suspended end of the culture section 3 is adjusted, the adjusting rope 4 pulls/pushes the non-suspended end of the culture section 3 to move forward and upward of the light receiving surface of the culture section 3, or pulls/pushes the non-suspended end of the culture section 3 to return to the initial position from the forward and upward direction of the culture section 3, and when the adjusting rope 4 is sequentially connected to the non-suspended ends of a plurality of culture sections 3 in the longitudinal direction thereof, the orientation of the non-suspended ends of the plurality of culture sections 3 can be adjusted at the same time.
In order to further enhance the adjustability of the adjusting device, in the above-mentioned embodiment, the adjusting device further comprises a rope length adjusting disk 7 with a horizontal axis, the rope length adjusting disk 7 can be located at the middle part of the longitudinal support rod 1, as shown in fig. 4 and 5, the rope length adjusting disk 7 is provided with a plurality of positioning parts 701 for connecting with the adjusting rope 4, for example, the positioning parts 701 are designed as positioning holes. Wherein, the distance between each positioning part 701 and the center of the rope length adjusting disc 7 can be the same or different. Specifically, the orientation of the non-suspended end of the culture section 3 can be adjusted by the string length adjusting plate 7 in the following ways:
in the first mode, the distances from the positioning parts 701 to the center of the rope length adjusting disc 7 are different, and the direction of the non-suspension end of the culture surface 3 can be adjusted by selecting different positioning parts 701, so that the culture surface 3 swings around the suspension end of the culture surface to adapt to the change of the solar altitude. For example, when the adjusting ropes 4 are fixed to the respective positioning portions 701 on the same horizontal line on the rope length adjusting disks 7, the non-suspended ends of the respective culture surfaces 3 will be displaced in the same vertical direction and in different horizontal directions; when the adjusting ropes 4 are fixed to the respective positioning portions 701 on the same vertical line on the rope length adjusting plate 7, the non-suspended ends of the respective culture surfaces 3 will be displaced in the same horizontal direction and in different vertical directions. In the second mode, the adjusting rope 4 is fixed to one of the positioning parts 701, and the direction of the non-suspension end of the culture surface 3 can be adjusted by rotating the rope length adjusting disc 7, so that the culture surface 3 swings around the suspension end thereof to adapt to the change of the solar altitude.
Therefore, the rope length adjusting disc 7 can conveniently adjust the vertical displacement and/or the horizontal displacement of the adjusting rope 4, so that the non-suspension end of the culture surface 3 swings to a proper position to adapt to the change of the sun height angle, and the adjustability of the adjusting device is further improved.
In order to further simplify the overall structure of the optical biological cell culture device, if the rope length adjusting disk 7 is not arranged, a plurality of positioning holes can be arranged at intervals in the middle part of the longitudinal support rod 1, when the orientation of the non-suspension end of the culture surface 3 needs to be adjusted, only the vertical displacement of the adjusting rope 4 needs to be adjusted and penetrates through the corresponding positioning holes, and meanwhile, the adjusting rope 4 moves along the length direction of the adjusting rope, so that the synchronous adjustment of the vertical displacement and/or the horizontal displacement of the non-suspension end of the culture surface 3 can be simultaneously realized, and the adjustability of the adjusting device is further enhanced.
In the above-mentioned adjusting device, except adopting two to adjust rope 4, can also adopt two to adjust the pole, two are adjusted the pole and are connected with the non-end that hangs of each culture section 3, and after the position adjustment of the non-end that hangs of culture section 3 finishes, two are adjusted the pole and all can be tied up or fix the relevant position at longitudinal support rod 1 through the segment rope through other commonly used fixed modes, or fix on other fixings. In order to conveniently adjust the vertical displacement and/or the horizontal displacement of the adjusting rod, the adjusting device is also provided with a rod length adjusting disc, and a hinged part used for being hinged with the adjusting rod is arranged on the rod length adjusting disc. Specifically, the orientation of the non-suspended end of the culture section 3 can be adjusted by two adjusting rods in the following ways: in the first mode, the rod length adjusting disc rotates, and the end part of the adjusting rod rotates along with the rod length adjusting disc, so that the vertical direction displacement and/or the horizontal direction displacement of the adjusting rod are changed, the position of the non-suspension end of the culture surface 3 is effectively adjusted, and the adjustability of the adjusting device is enhanced. In the second mode, the adjusting rod is fixed at different positions of the rod length adjusting disc, so that the vertical displacement and/or the horizontal displacement of the adjusting rod are/is changed, the direction of the non-suspension end of the culture surface 3 is effectively adjusted, and the adjustability of the adjusting device is enhanced.
In the above embodiment, the adjusting device can effectively adjust the orientations of the non-suspended ends of the culture sections 3 at the same time, thereby saving the time for adjusting the culture sections 3 individually. In order to further save the time for adjusting the plurality of culture sections 3, the culture sections 3 may be arranged at equal intervals, and the light receiving surfaces of the plurality of culture sections 3 disposed on the same support may receive the same light energy, so that the adjusting device may simultaneously adjust the orientation of the non-suspended end of each culture section 3 to the proper position, thereby further saving the adjustment time.
According to the embodiment, when the culture surface 3 is a flexible surface, the adjusting device and the adjusting method can effectively adjust the orientation of the non-suspension end of the flexible culture surface 3, so that the light utilization rate and the light splitting rate are effectively improved. On the other hand, when the culture section 3 is a rigid section, the orientation of the non-suspended end of the culture section 3 is adjusted by the following adjusting means in order to effectively adjust the orientation of the non-suspended end of the rigid culture section 3.
When the culture section 3 is a rigid section, the top end is a hanging end and the bottom end is a non-hanging end. The adjustment means for adjusting the rigid culture surface 3 may be of flexible construction. Specifically, when the adjusting device is of a flexible structure, for example, the adjusting device comprises two long ropes in rope form, the two long ropes are both connected with the non-suspension end of each culture surface 3, and after the orientation of the non-suspension end of each culture surface 3 is adjusted, the two long ropes are both tied to the corresponding positions of the longitudinal support rods 1. Because the long rope is flexible structure, so can freely adjust the position of the non-end that hangs of culture medium 3, when the long rope drove the non-end that hangs of culture medium 3 and moves, the non-end that hangs of culture medium 3 will be around its end swing that hangs to change the position of culture medium 3, in order to adapt to the change of solar altitude angle and azimuth, improve light utilization ratio and spectral rate.
In addition, in order to further ensure the growth rate of the photobiological cells, the culture section 3 may have various structures, for example, the culture section 3 may have a single-layer structure, or the culture section 3 may have a multi-layer structure formed by joining a plurality of single-layer structures independent of each other, or the culture section 3 may have a multi-layer structure formed by integrally joining a plurality of single-layer structures to each other. Because different materials have different properties, when the culture surface 3 adopts a multilayer structure, the properties of the different materials can be integrated, and the comprehensive properties of the culture surface 3 are effectively improved, so that the growth speed of the photobiological cells is further ensured. For example, when a material with good cell adsorption performance and a material with good water conductivity are combined or combined to form the culture surface 3 with a multilayer structure, the culture surface 3 not only has good adhesiveness with photobiological cells, but also has good water conductivity, so that the growth requirement of the photobiological cells is met; for another example, when a material suitable for growth of photobiological cells is bonded or combined with a material having a light reflecting function to form the culture section 3 having a multi-layered structure, the culture section 3 can be adapted to culture photobiological cells and the back surface of the culture section 3 can be made light-reflecting.
It should be noted that, in the embodiment of the present invention, it is preferable that the back light surface of the culture surface 3 is a light reflecting surface made of a light reflecting material, so that the scattered light or/and the direct light irradiated on the light reflecting surface can be reflected to the light receiving surface of the adjacent culture surface 3, thereby increasing the light receiving amount of the adjacent culture surface 3, further increasing the light utilization rate, ensuring the light energy required by the photo-biological cells, and further ensuring the growth rate of the photo-biological cells. The back surface of the culture section 3 is a surface that receives little sunlight, receives mainly scattered light and a small amount of direct light, and is opposite to the light-receiving surface of the culture section 3.
The culture section 3 can be formed in various ways, for example, the culture section 3 can be woven, non-woven, series-connected or directly formed, wherein the culture section 3 made of sponge, polyvinyl alcohol or the like is representative of the directly formed structure. In addition, the culture section 3 may be designed in various shapes, for example, the culture section 3 may be a regular shape such as a rectangle or other irregular shapes, and the thickness uniformity thereof are not limited, as shown in fig. 1 and 2, when the culture section 3 is a rectangle, the top side thereof is a hanging end and is connected to the transverse support bar 2, and the bottom side thereof is a non-hanging end and is connected to the adjusting device.
In addition, in order to make the photo-biological cells grow in a more suitable environment, for example, in a suitable nutrient solution environment, the photo-biological cell culture apparatus is further provided with a liquid distributor 8, the liquid distributor 8 is connected with a liquid supplementing device 6, as shown in fig. 6, the liquid distributor 8 distributes the nutrient solution supplied by the liquid supplementing device 6 onto the light receiving surface of the culture surface 3 to supply the nutrient solution to the photo-biological cells attached to the culture surface 3, usually, the nutrient solution comprises water, nutrient or sterilizing agent, and the liquid distributor 8 is connected with an outlet of the liquid supplementing device 6 in a manner of an easily detachable movable link, such as plug-in quick connection, screw-in rotary connection and the like. The inlet of the liquid supplementing device 6 can be connected with a liquid collector 5, and nutrient solution which is not used by the photobiological cells from the liquid distributor 8 is collected in the liquid collector 5, namely the liquid collector 5 is used for receiving the nutrient solution flowing out of the culture surface 3 and is reused by the liquid supplementing device 6. In order to remove germs, insect pests, impurities and algae cells in the nutrient solution entering the liquid supplementing device 6, a filtering device is connected at the inlet of the liquid supplementing device 6.
It should be noted that if the amount of nutrient solution provided by the liquid distributor 8 to the photobioreactor cells on the culture surface 3 is small or just can meet the growth requirement of the photobioreactor cells, i.e., if no nutrient solution not utilized by the photobioreactor cells exists or cannot flow out of the culture surface 3, the liquid collector 5 is not needed, and at this time, the inlet of the liquid supplementing device 6 can be communicated with the external nutrient solution supply device, and the nutrient solution can be provided to the photobioreactor cells by the external nutrient solution supply device. Further, if the desired nutrient solution can be obtained from the photobiological cells adhering to the culture section 3 by the movement or siphon action of the culture section 3, only the liquid trap 5 may be provided, and the nutrient solution required for the photobiological cells may be stored in the liquid trap 5.
In the above embodiments, the liquid distributor 8 may have various structural shapes, for example, the liquid distributor 8 has a tubular structure, and is provided with a nutrient solution outlet, which may be one or more overflow ports, and the nutrient solution outlet may also be a small hole. For another example, the liquid distributor 8 is a nozzle structure. For another example, the liquid distributor 8 is a hollow structure made of a microporous material, such as microporous film, microporous mesh, microporous ceramic, etc., and the hollow structure may be a closed or open tube, tank, etc.
When the liquid distributor 8 is a hollow body structure, the outside of the hollow body of the liquid distributor 8 is in contact with the top end of the culture surface 3, the culture surface 3 or the photo-biological cell attachment surface thereon is made of fiber material or other material capable of generating capillary action (the algae cells themselves can be regarded as a material capable of generating capillary action), or a fiber material body such as fiber fabric or other material capable of generating capillary action is added between the outside of the hollow body and the culture surface 3. When the nutrient solution flows through the middle part of the hollow body of the liquid distributor 8, under the capillary attraction action of the fiber material, the nutrient solution slowly or according to the requirement supplies the required nutrient solution to the photo-biological cell attachment surface of the culture surface 3, which not only can lead the photo-biological cells to obtain the required nutrient solution, but also can simplify the structure of the photo-biological cell device and reduce the cost.
In order to facilitate the adhesion of the photo-biological cells to the surface and/or inside of the culture section 3, an inoculation device is further provided in the above-mentioned embodiment. The inoculating device has a cell storage region or a cell supply port, and may be in contact with the culture surface 3 or not. When the culture section 3 is moved, the inoculating device attaches the photobiological cells to the surface and/or inside the culture section 3. In addition, the inoculating device can be connected with a moving device, and the moving device drives the inoculating device to move relative to the culture surface 3, so that the photobiological cells supplied by the cell storage area or the cell supply port are attached to the surface or/and the interior of the culture surface 3. The inoculation device may be used in various inoculation modes, such as spraying, sprinkling, dipping, smearing, and suction filtering.
In order to collect the mature photo-biological cells, in the above embodiment, a peeling means for peeling the mature photo-biological cells off the surface of the culture section 3 and/or inside the culture section is further provided, and the peeling means peels the mature photo-biological cells off the culture section 3. The stripping means may or may not be in contact with the culture surface 3. When the culture surface 3 is moved, the stripping device enables the mature photobiological cells to be separated from the surface or/and the interior of the culture surface 3, and the separated photobiological cells are contained in the container. In addition, the peeling device can be connected with a moving device, and the moving device drives the peeling device to move relative to the culture surface 3, so that all or part of mature photobiological cells attached to the culture surface 3 are separated from the surface or/and the interior of the culture surface 3. The collecting method of the mature photobiological cells can be various, for example, scraping, squeezing, blowing (such as air blowing, gas-liquid blowing), spraying (such as liquid spraying, gas-liquid spraying), flushing and the like.
In order to facilitate the installation, removal and adjustment of the overall position of the culture section 3, the above-described optical biological cell culture apparatus is further provided with a transport assembly for transporting the culture section 3 and a pick-up device for lifting or lowering the culture section 3. In which work such as inoculation of photobiological cells, collection of photobiological cells, and adjustment of the distance between adjacent culture surfaces 3 is realized by moving the culture surfaces 3.
Three specific examples are listed below to describe the above examples in more detail.
Example one
In the embodiment, the optical biological cell culture device comprises a bracket, wherein the bracket comprises four vertically arranged longitudinal support rods 1 and two parallel and transversely arranged transverse support rods 2, wherein the longitudinal support rods 1 are made of stainless steel upright columns with the height of 2000mm and the diameter of 60mm, projection points of the four longitudinal support rods 1 on the ground can be connected into a rectangle, the distance between the two longitudinal support rods 1 at the end points of the long side of the rectangle is 4000mm, and the distance between the two longitudinal support rods 1 at the end points of the short side of the rectangle is 1000 mm; the transverse supporting rod 2 is made of nylon ropes with the diameter of about 30mm, and the two ends of each nylon rope are connected with the top end of the longitudinal supporting rod 1 at the end point of the rectangular long side. The middle part of each longitudinal support rod 1 is provided with a series of round holes with the interval of about 20mm and the diameter of 5mm, the round holes are the positioning parts 701, and the round holes at the bottommost part of the longitudinal support rods 1 are about 500mm away from the ground.
The optical biological cell culture device also comprises a culture surface 3 and an adjusting rope 4, wherein the culture surface 3 is formed by compounding superfine fiber materials and TPU (Thermoplastic polyurethanes) films, the light receiving surface of the culture surface is the superfine fiber materials, and the backlight surface is the TPU materials; as shown in FIG. 6, the culture section 3 is rectangular, 1500mm long and 1000mm wide, and has a hanging end at the top side and a non-hanging end at the bottom side; wherein, one end of the hanging end of the culture surface 3 is connected with one transverse supporting rod 2, the other end is connected with the other transverse supporting rod 2, the distance between the adjacent culture surfaces 3 is 150mm, and the number of the culture surfaces 3 is 20; the adjusting ropes 4 are two nylon ropes with a diameter of about 5mm, each adjusting rope 4 is connected with one end of the non-suspension end of each culture surface 3, the tail end of each adjusting rope 4 is connected with the corresponding positioning part 701 on the corresponding longitudinal support rod 1, for example, the first positioning part 4 is connected with the positioning part 701 and then is bound on the corresponding longitudinal support rod 1. When sunlight irradiates the ground between the adjacent culture surfaces 3, the non-suspension ends of the culture surfaces 3 meet the requirements of vertical displacement and/or horizontal displacement by adjusting the positioning parts 701 connected with the ropes 4 at different heights, so that the orientation of the non-suspension ends of the culture surfaces 3 is adjusted, the ground between the adjacent culture surfaces 3 is not irradiated by the sunlight, and the light utilization rate is improved.
In addition, the optical biological cell culture device also comprises a liquid distributor 8 and a liquid supplementing device 6. As shown in fig. 6, the liquid distributor 8 is a tubular structure made of a mixed fiber material, and has a length of about 1000mm and an inner diameter of about 15 mm; the aperture of the nutrient solution outlet on the liquid distributor 8 is about 15um, and the aperture ratio is more than 80 percent; the liquid distributor 8 is fixed at the suspension end of the culture surface 3 after being wrapped by the superfine fiber surface, the liquid distributor 8 is tightly contacted with the superfine fiber layer, one end of the liquid distributor 8 is sealed, and the other end is connected with the liquid supplementing device 6. Wherein, the liquid supplementing device 6 is a flow regulating valve, one end of which is connected with the liquid distributor 8, and the other end is connected with an external nutrient solution supply tank.
Example two
In the embodiment, the optical biological cell culture device comprises a bracket, wherein the bracket comprises four vertically placed longitudinal support rods 1 and two parallel and transversely placed transverse support rods 2, the longitudinal support rods 1 are made of stainless steel upright columns with the height of 2000mm and the diameter of 60mm, projection points of the four longitudinal support rods 1 on the ground can be connected into a rectangle, the distance between two longitudinal support rods 1 at the end points of the long side of the rectangle is 4000mm, and the distance between two adjacent longitudinal support rods 1 at the end points of the short side of the rectangle is 1000 mm; the top ends of the longitudinal support rods 1 are respectively provided with a pulley, the diameter of the pulley is 100mm, and the pulley can be in a locking state according to requirements. The transverse supporting rod 2 is made of a nylon rope with the diameter of about 30 mm; each nylon rope is provided with 20 annular knots, the interval between every two adjacent annular knots is 150mm, the diameter of each annular knot is slightly larger than that of an upper suspension member 9 connected with the top end of the culture surface 3, and the top end of the culture surface 3 is a suspension end; each nylon rope is respectively wound around the pulleys at the top ends of the two longitudinal support rods 1 and then connected end to form a closed ring.
The photo-biological cell culture device also comprises a culture surface 3, an upper suspension member 9 and an adjusting device, wherein the culture surface 3 is formed by matching a nylon net and cotton canvas, the light receiving surface of the culture surface is a layer of nylon net, the backlight surface is a layer of cotton canvas, the length of the nylon net is 1500mm, the width of the cotton canvas is 1000mm, and the suspension end of the culture surface 3 is tightly connected with the upper suspension member 9. Wherein, the upper suspension component 9 is an organic glass rod, the diameter of which is 20mm, and the length of which is 2000 mm; the hanging end of the culture surface 3 is tightly connected with the middle part of the upper hanging member 9, and the two ends of the upper hanging member 9 are respectively inserted into the corresponding annular knots on the two transverse supporting rods 2.
The adjusting device comprises two adjusting ropes 4 and a rope length adjusting disc 7, the two adjusting ropes 4 are made of nylon ropes with the diameter of 10mm, each adjusting rope 4 is connected with the bottom end of each culture surface 3, and the bottom end of each culture surface 3 is a non-suspension end; one end of the adjusting rope 4 is fixed to the non-suspended end of the outermost (e.g., the last) culture section 3, and the other end is connected to a positioning section 701 of the rope length adjusting disk 7.
Each rope length adjusting disk 7 is connected to one end of the adjusting rope 4, for example, the adjusting rope 4 is connected to a certain positioning part 701 on the rope length adjusting disk 7 and then is bound to the rope length adjusting disk 7; the rope length adjusting disc 7 is arranged in the middle of the longitudinal support rod 1, and the axis of the rope length adjusting disc 7 and the non-suspension end of each culture surface 3 are positioned on the same horizontal plane; the rope length adjusting disk 7 is made of wood material, the diameter is 200mm, the thickness is 30mm, holes with the diameter of 10mm are evenly distributed on the rope length adjusting disk, the holes are the positioning parts 701, and the center distance between adjacent holes is about 25 mm. When sunlight irradiates the ground between the adjacent culture surfaces 3, the pulley is locked, and then the positioning part 701 connected with the adjusting rope 4 is adjusted or replaced, or the rope length adjusting disc 7 is rotated, so that the non-suspension end of each culture surface 3 meets the requirements of vertical displacement and/or horizontal displacement, and further the position adjustment of the culture surfaces 3 is realized; when the position of each culture section 3 is adjusted, the degrees of adjustment at both ends of the non-suspended end of the culture section 3 are made the same.
In this embodiment, the optical biological cell culture apparatus can also select another way to adjust the position of the culture surface 3, namely: the two pulleys on one side are locked, and the two pulleys on the other side are not locked, so that the horizontal displacement of one end of the non-suspension end of the culture surface 3 is larger than that of the other end, or the vertical displacement of one end of the non-suspension end of the culture surface 3 is larger than that of the other end, and the culture surface 3 is twisted. In addition, can also only make horizontal bracing piece 2 along its length direction's removal through adjusting the relative displacement of two horizontal bracing pieces 2, and then realize the wrench movement of culture face 3 and adjust to reduce the subaerial illumination area between the adjacent culture face 3 or cultivate the shadow area on the face 3, improve light utilization rate and spectral rate.
In addition, the optical biological cell culture device also comprises a liquid distributor 8, a liquid supplementing device 6 and a liquid collecting device 5. Wherein, the liquid distributor 8 is a hollow organic glass tube with the diameter of about 10mm, the liquid distributor 8 is evenly provided with through holes with the interval of 10mm and the diameter of about 1mm, and the liquid distributor 8 is connected with the upper suspension member 9, thereby continuously providing required nutrient solution for the photo-biological cells on the culture surface 3. One end of the liquid distributor 8 is sealed, the other end of the liquid distributor is connected with the liquid supplementing device 6, the liquid supplementing device 6 is a submersible pump and is immersed in the nutrient solution in the liquid collector 5, and the nutrient solution in the liquid collector 5 is continuously conveyed to the liquid distributor 8. The liquid collector 5 may be a PVC (Polyvinyl chloride) water tank having a length of 3700mm and a width of 2000mm for receiving the nutrient solution flowing down from the culture section 3 or storing the nutrient solution required for the photo-biological cells attached to the culture section 3.
EXAMPLE III
In this embodiment, the photo-biological cell culture device comprises a culture surface 3, a lower suspension member 10 and a regulating device.
Wherein, the culture section 3 is made of a chemically synthesized film-like material, that is, the culture section 3 is a composite film and is provided with columnar protrusions with a length of about 3mm and a diameter of less than 1mm on the surface thereof. The culture section 3 has a rectangular shape with a length of 1500mm and a width of 1000mm, and the non-suspended end thereof is closely connected with a lower suspension member 10. The lower suspension member 10 is a bamboo stick with a diameter of 10mm and a length of about 1000mm, and the middle part of the lower suspension member 10 is connected with the adjusting rope 4.
In addition, the adjusting device comprises adjusting ropes 4 and a rope length adjusting disc 7, the adjusting ropes 4 are made of nylon ropes with the diameter of about 10mm, and each adjusting rope 4 is connected with the middle part of the lower suspension member 10; one end of the adjusting rope 4 is fixed to the lower hanging member 10 at the bottom end of the outermost (e.g., the last) culture section 3, and the other end is connected to a positioning section 701 of the rope length adjusting disk 7. The rope length adjusting plate 7 is disposed with its axis horizontal, and the rope length adjusting plate 7 is connected to one end of the adjusting rope 4, for example, the adjusting rope 4 is connected to a certain positioning portion 701 of the rope length adjusting plate 7 and then bound to the rope length adjusting plate 7. The rope length adjusting disk 7 is made of PVC material, the diameter is 150mm, the thickness is 30mm, through holes with the diameter of 15mm are evenly distributed on the rope length adjusting disk, the through holes are the positioning parts 701, the center distance between the adjacent through holes is about 30mm, the rope length adjusting disk 7 is installed in the middle of the longitudinal support rod 1, and the axis of the rope length adjusting disk 7 and the axis of the lower suspension member 10 at the bottom end of each culture surface 3 are in the same horizontal plane. When sunshine shines subaerial between the adjacent culture face 3, the accessible rotates long adjusting disk 7 of rope, or changes the location portion 701 of being connected with first regulation 4, makes the non-of each culture face 3 hang the requirement that the end all satisfies vertical direction displacement and/or horizontal direction displacement, and then realizes the regulation to the position of the non-of culture face 3 hanging the end, makes subaerial not having sunshine between the adjacent culture face 3 shine, improves light utilization ratio.
The optical biological cell culture device in this embodiment further includes a support, which is the same as the support in the first embodiment, and is not described herein again.
The optical biological cell culture device in this embodiment further includes a liquid distributor 8, a liquid supplementing device 6 and a liquid collecting device 5, which are the same as the corresponding components in the second embodiment and are not described again.
In summary, in the optical biological cell adjusting device of the present invention, the support and the adjusting device are arranged to adjust the orientation of the non-suspended end of the culture surface according to the change of the solar altitude angle and the solar azimuth angle, so that the non-suspended end of the culture surface moves towards the light receiving surface of the culture surface, thereby reducing the light loss caused by the sunlight leaking on the ground through the gap between the adjacent culture surfaces and improving the light utilization rate. When the culture surface is a flexible surface, the culture surface can change between a straight surface, a curved surface and a straight surface along with the change of the altitude angle or the azimuth angle of the sun, so that no light leakage between the adjacent culture surfaces is ensured, the light waste caused by the direct irradiation of sunlight on the ground between the adjacent culture surfaces is reduced and even avoided, the light receiving surface of the culture surface obtains more light energy, the light receiving area of the culture surface is larger, and the light utilization rate is improved; and shadow generated on the adjacent culture surface can be reduced or even avoided, and the maximum light splitting is realized, so that the light splitting rate is improved. In addition, the cost of the culture surface can be reduced, and the cost of the whole optical biological cell culture device can be further reduced.
When the backlight surface of the culture surface is made of a light-reflecting material, the backlight surface can reflect light irradiated on the backlight surface to the light-receiving surface of the adjacent culture surface, so that the light receiving and utilizing efficiency of the optical biological cell culture device is further improved.
Nutrient solution required by the photobiological cells on the culture surface can be supplied as required through the liquid distributor 8, the liquid supplementing device 6 or the liquid collector 5 and the like, different liquid supply speeds are provided according to the water evaporation speeds of each day and different seasons, and the impact on the photobiological cells caused by overlarge liquid supply amount is avoided, so that the photobiological cells are not easy to fall off from the surface or/and the inside of the culture surface.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (12)
1. A photobiological cell culture device is characterized by comprising a bracket, wherein at least two culture surfaces which are arranged at intervals are hung on the bracket, the non-hanging ends of the culture surfaces are connected with an adjusting device for adjusting the direction of the non-hanging ends, the non-hanging ends of the culture surfaces swing around the hanging ends of the culture surfaces and block sunlight irradiated on the ground between the adjacent culture surfaces through the adjusting device, the culture surfaces are flexible surfaces, the top end parts of the culture surfaces are hanging ends, the bottom end parts of the culture surfaces are non-hanging ends, the bracket comprises four vertical support rods which are vertically arranged, the top ends of two vertical support rods are connected with one transverse support rod, the top ends of the other two vertical support rods are connected with the other transverse support rod, and the two transverse support rods are both connected with the hanging ends of the culture surfaces, the non-suspension end of each culture surface is connected with the adjusting device; the adjusting device comprises two adjusting ropes or two adjusting rods.
2. The photo-biological cell culture device according to claim 1, wherein the hanging end of the culture surface is connected with an upper hanging member, and two ends of the upper hanging member are connected with the two transverse support rods; or/and the non-suspension end of the culture surface is connected with a lower suspension member, and two ends of the lower suspension member are connected with the adjusting device.
3. The device for culturing the biological cells of claim 1, wherein the two adjusting ropes are tied to the corresponding positions of the longitudinal support rods after the orientation of the non-suspended end of the culture section is adjusted.
4. The device of claim 3, further comprising a cord length adjusting disk disposed with its axis horizontal, wherein the cord length adjusting disk is connected to the adjusting cord.
5. The device of claim 1, wherein the adjustment of the orientation of the non-suspended end of the culture section is completed and both of the adjustment rods are fixed to the corresponding positions of the longitudinal support rods.
6. The device of claim 5, further comprising a lever length adjustment plate, wherein the lever length adjustment plate is provided with a hinge portion for hinging the adjustment lever.
7. The photobioreactor cell culture apparatus as claimed in claim 1, wherein the culture section is a single-layer structure, or a multi-layer structure in which a plurality of single-layer structures are joined or bonded.
8. The photobioreactor cell culture apparatus of claim 1, wherein the back side of the culture surface is a light-reflective surface.
9. The photo-biological cell culture device according to claim 1, further comprising a liquid distributor for providing nutrient solution to the photo-biological cells attached to the culture surface, wherein the liquid distributor is connected with a liquid supplementing device.
10. The device for culturing the optical biological cells as claimed in claim 9, wherein the liquid distributor is a tubular structure and is provided with a nutrient solution outlet; or the liquid distributor is a hollow body structure made of microporous materials.
11. The photobioreactor apparatus of claim 10, further comprising a liquid trap for collecting nutrient solution flowing from the culture surface.
12. The photo-biological cell culture device according to claim 1, further comprising an inoculating device for attaching photo-biological cells to the culture surface, a peeling device for peeling mature photo-biological cells off the culture surface, a moving device for moving the inoculating device or/and the peeling device, a conveying assembly for conveying the culture surface, and an extracting device for lifting or lowering the culture surface.
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