CN106701562A - Intelligent biochemical incubator - Google Patents
Intelligent biochemical incubator Download PDFInfo
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- CN106701562A CN106701562A CN201710032118.6A CN201710032118A CN106701562A CN 106701562 A CN106701562 A CN 106701562A CN 201710032118 A CN201710032118 A CN 201710032118A CN 106701562 A CN106701562 A CN 106701562A
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- incubator
- mobile terminal
- crystal oscillator
- box body
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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- General Engineering & Computer Science (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses an intelligent biochemical incubator which comprises a box body, a central controller, a refrigeration device, a heating device, a sensing system, a wireless WIFI (Wireless Fidelity) module and a mobile terminal module, wherein an observation window is formed in the door of the box body; an incubator is arranged inside the box body; a plurality partitioning layer plates are arranged inside the incubator; the refrigeration device, the heating device and the sensing system are all connected with the central controller. The intelligent biochemical incubator is reasonable in box body design, simple in structure and economic and practical in manufacturing materials. Due to arrangement of the sensing system inside the incubator, and the high sensitivity of a sensor, technical parameters inside the incubator can be conveniently understood by operators, a relatively appropriate ecological environment can be simulated, and biochemical culture can be relatively well facilitated. Due to arrangement of the display and the control panel above the box body, convenient operation can be achieved; as the WIFI module is in wireless connection with the mobile terminal, various parameters of the incubator can be understood remotely in real time, experiments can be made by testers conveniently, and remote control on the incubator can be also achieved through the mobile terminal.
Description
Technical field
The present invention relates to intelligent medical technique with the apparatus field, in particular it relates to a kind of Intelligent biochemistry incubator.
Background technology
The culture experiment of microorganism generally needs different condition of culture, and this is accomplished by using the biochemical culture of difference in functionality
Case.Biochemical cultivation case temperature-control range now on the market is general at 5~50 DEG C, and temperature control error is 0.5 DEG C, by making for a long time
With often there are the failures such as accuracy of temperature control and humidity out of control, so as to cause biochemical culture the failure of an experiment.In fact, biochemical culture
The Temperature and Humidity Control of case breaks down, and main cause is that incubator process is used for a long time, causes ventilating duct remaining dust etc.
Impurity, causes vent passages to narrow, and air circulation is reduced.Existing biochemical cultivation case is typically all using the refrigerant compression of family expenses
Machine freezes, its complex structure, once break down, maintenance difficult, and noise is larger.
The content of the invention
For the above-mentioned deficiency of prior art, the invention provides a kind of Intelligent biochemistry incubator, to solve above-mentioned skill
Art problem.
In order to solve the above-mentioned technical problem, the present invention is achieved by the following technical programs：
A kind of Intelligent biochemistry incubator, including casing, central controller, refrigerating plant, heater, sensor-based system, nothing
Line WIFI module and mobile terminal module；The casing is provided with observation window in chamber door, and the box house is provided with incubator,
Incubator is provided with the layering of polylith interlayer board；Refrigerating plant, heater and sensing system are connected with central controller,
The wireless WIFI module is connected with central controller, the wireless WIFI module and mobile terminal module wireless connection, refrigeration
Device and heater are connected with incubator respectively.
Relative to prior art, beneficial effects of the present invention：
1st, rationally, simple structure, materials being fabricated is economical and practical for cabinet design of the invention.
2nd, sensing system is provided with incubator of the present invention, the sensitivity of sensor is high, aspect operating personnel understand case
The technical parameter in internal portion, the ecological environment that simulation is more suitable for is more beneficial for biochemical culture.
3rd, the present invention is provided with display and control panel, convenient operation, WIFI module and mobile terminal above casing
Wireless connection, remotely can in real time understand the various parameters of incubator, facilitate experimenter to carry out experiment, it is also possible to by movement
Terminal-pair incubator carries out remote control.
4th, gas sensor of the invention reach 0.105 in the detection limit concentration of hydrogen sulfide, ethene and substituted benzene,
0.946th, 0.835ppm, can detect substituted benzene, show that the detecting domains of the gas sensor are wider, 3 kinds of gases of detection
Response time respectively reaches 625,693,736ms, show that the reaction time of the gas sensor is short, test result is more accurate, can
To be applied to vast field of gas detection, can be used for the gas-monitoring of biochemical cultivation case.
Brief description of the drawings
Accompanying drawing herein is merged in specification and constitutes the part of this specification, shows and meets implementation of the invention
Example, and be used to explain principle of the invention together with specification.
Fig. 1 is structural representation of the invention.
Wherein, casing -60, observation window -61, interlayer board -62, ventilating opening 63, central controller -70, refrigerating plant -71,
Heater -72, wireless WIFI module -73, display -74, control panel -75, temperature sensor -76, humidity sensor -
77, gas sensor -78.
Fig. 2 is the structural representation of gas sensor of the invention.
Wherein, frequency counter -781, oscillator -782 contrast crystal oscillator -7831, target crystal oscillator -7832, air inlet 784.
Specific embodiment
The invention will be further described with the following Examples.
Fig. 1 is a kind of Intelligent biochemistry incubator according to an exemplary embodiment, as shown in figure 1, including casing, in
Centre controller, refrigerating plant, heater, sensor-based system, wireless WIFI module and mobile terminal module；The casing is in chamber door
Observation window is provided with, the box house is provided with incubator, and incubator is provided with the layering of polylith interlayer board；Refrigerating plant, plus
Thermal and sensing system are connected with central controller, and the wireless WIFI module is connected with central controller, described
Wireless WIFI module and mobile terminal module wireless connection, refrigerating plant and heater are connected with incubator respectively.
Preferably, the central controller also includes display and control panel.
Preferably, the sensor-based system includes temperature sensor, humidity sensor and gas sensor.
It is highly preferred that the mobile terminal module is mobile phone or panel computer.
Sensing system is provided with incubator of the present invention, the sensitivity of sensor is high, aspect operating personnel understand casing
Internal technical parameter, the ecological environment that simulation is more suitable for, is more beneficial for biochemical culture.The present invention is provided with aobvious above casing
Show device and control panel, convenient operation, WIFI module and mobile terminal wireless connection, remotely can in real time understand each of incubator
Parameter is planted, facilitates experimenter to carry out experiment, it is also possible to carry out remote control to incubator by mobile terminal.
The air-sensitive biography that a kind of Intelligent biochemistry incubator provided in embodiments of the invention is used is described below
The structure and type of sensor, in this embodiment, gas sensor includes gas sensor shell and microprocessor.The air-sensitive is passed
Detection air chamber, multiple oscillators, frequency counter and beat-frequency meter are provided with sensor shell；The detection air chamber is provided with air inlet
Mouthful, detection air chamber in be provided with contrast crystal oscillator and target crystal oscillator, contrast crystal oscillator and target crystal oscillator respectively with corresponding oscillator
Electrically connected with frequency counter, the two ends of the beat-frequency meter are connected to frequency counter, the 3rd end of the beat-frequency meter with it is micro-
Processor is electrically connected；The target crystal oscillator surface has air-sensitive coating, and the air-sensitive coating is that nano polyaniline-activated carbon is applied
Layer.
The preparation process of the nano polyaniline-activated carbon coating of the gas sensor will be introduced in following part, including following
Step：
S1. aniline and ammonium persulfate are mixed to join in hydrochloric acid solution, are vibrated in ultrasound environments, and standing obtains final product nanofiber
Polyaniline；
S2. nano active carbon powder and Nano-fibrous PANI are mixed, is vibrated in ultrasound environments, in the bar of magnetic agitation
Ammonium persulfate hydrochloric acid solution is slowly added under part；
S3. product is placed in Fluorine containing olefine atmosphere, and irradiation polymerization is carried out in the irradiation field of cobalt -60；
S4. product filtering, washing and vacuum drying are obtained final product into nano polyaniline-activated carbon.
A specifically embodiment is provided below：
Quantitative aniline and ammonium persulfate are added in hydrochloric acid solution, aniline monomer is kept with the material of ammonium persulfate
The ratio between amount is 5:5-10, then vibrates 40min in solution is placed in into ultrasound, stands 3-7h and obtains Nano-fibrous PANI；Will
The hydrochloric acid solution mixing of a certain proportion of nano active carbon powder and Nano-fibrous PANI, is placed in sonic oscillation in Ultrasound Instrument
30min, makes aniline be adsorbed on activated carbon, then dissolves ammonium persulfate into hydrochloric acid solution, under conditions of magnetic agitation, incites somebody to action
Ammonium sulfate acid solution is added dropwise in the activated carbon hydrochloric acid solution for having adsorbed polyaniline with the slow-paced of 15ul/s, control temperature
It is 4 DEG C to spend, and reacts 15h, in order to increase the adsorption ratio of the target product of gained, above-mentioned solution is placed into non-Fluorine containing olefine
In atmosphere, irradiation polymerization is carried out in the irradiation field of cobalt -60, the irradiation polymerization time is 5~9h, and irradiation dose is 300~400Gy/
Min, question response terminates rear vacuum filtration, dry in 60 DEG C of vacuum by product deionized water, absolute ethanol washing to colourless
It is dry, that is, obtain nano polyaniline-activated carbon.
For the ease of data tracing and access data, micro-display and control panel are additionally provided with the microprocessor, be
Cost-effective and profile of the gas sensor is more attractive in appearance.
Preferably, the gas sensor includes gas sensor shell and microprocessor；The gas sensor shell
Inside it is provided with detection air chamber, multiple oscillators, frequency counter and beat-frequency meter；The detection air chamber is provided with air inlet, detects
Be provided with air chamber contrast crystal oscillator and target crystal oscillator, contrast crystal oscillator and target crystal oscillator respectively with corresponding oscillator and frequency meter
Number device is electrically connected, and the two ends of the beat-frequency meter are connected to frequency counter, the 3rd end and the microprocessor electricity of the beat-frequency meter
Connection；The target crystal oscillator shows that, with air-sensitive coating, the air-sensitive coating is nano polyaniline-activated carbon coating；The inspection
Survey and 1-3 air inlet is provided with air chamber, 1-3 contrast crystal oscillator, 1-3 target crystal oscillator.
In the present embodiment, as shown in Fig. 2 being provided with 1 air inlet on detection air chamber, 2 contrast crystal oscillators, 2 targets
Crystal oscillator, wherein, the surface of target crystal oscillator is coated with air-sensitive coating, and the air-sensitive coating is made up of nano polyaniline-activated carbon, due to
The air-sensitive performance of gas sensor depends primarily on the air-sensitive coating of its surface coating.
Gas sensor scribbles gas sensitization film, when the absorption of tested gas molecule is on gas sensitization film, the matter of film
Amount increases, and causing the resonant frequency of quartz crystal oscillator reduces.With high sensitivity, Monitoring lower-cut can reach gas concentration
Even ppb grades of ppm.Gas sensor has the advantages that simple structure, with low cost, sensitivity be high, good stability.
Experimental example
By following experimental example, the nano polyaniline-activated carbon used the present invention as gas sensitive coating air-sensitive
The performance test of sensor, including response time and the limting concentration of test, further appreciate that the gas sensing that the present invention is used
The application domain scope of device.Object gas are common hydrogen sulfide, ethene and substituted benzene that may be present in biochemical culture, often
Plant gas test 3 times, calculate its average value, count the test result of a technical indicator, as a result as shown in table 1；
Table 1
| Limting concentration (ppm) | Response time (ms) | |
| Hydrogen sulfide | 0.105 | 625 |
| Ethene | 0.946 | 693 |
| Substituted benzene | 0.835 | 736 |
The limting concentration of in summary different test target gas and response time, the as shown by data of table 1 are of the invention complete
Intelligent life incubator can detect hydrogen sulfide, ethene and substituted benzene, and sensitivity is high, and fast response time is reproducible,
And stability is preferably, the detection limit concentration of hydrogen sulfide, ethene and substituted benzene reaches 0.105,0.946,0.835ppm, can
To detect substituted benzene, show that the detecting domains of the gas sensor are wider, the response time for detecting 3 kinds of gases respectively reaches
625th, 693,736ms, shows that the reaction time of the gas sensor is short, and test result is more accurate, can apply to vast gas
Detection field, can be used for the gas-monitoring of biochemical cultivation case.
Finally it should be noted that the above embodiments are merely illustrative of the technical solutions of the present invention, rather than the present invention is protected
The limitation of scope is protected, although being explained to the present invention with reference to preferred embodiment, one of ordinary skill in the art should
Work as understanding, technical scheme can be modified or equivalent, without deviating from the reality of technical solution of the present invention
Matter and scope.
Claims (4)
1. a kind of Intelligent biochemistry incubator, it is characterised in that including casing, central controller, refrigerating plant, heater, biography
Sensing system, wireless WIFI module and mobile terminal module；The casing is provided with observation window in chamber door, and the box house is set
There is incubator, incubator is provided with the layering of polylith interlayer board；Refrigerating plant, heater and sensing system with central control
Device is connected, and the wireless WIFI module is connected with central controller, and the wireless WIFI module and mobile terminal module are wireless
Connection, refrigerating plant and heater are connected with incubator respectively.
2. Intelligent biochemistry incubator according to claim 1, it is characterised in that the central controller also include display and
Control panel；The mobile terminal module is mobile phone or panel computer.
3. Intelligent biochemistry incubator according to claim 2, it is characterised in that the sensor-based system include temperature sensor,
Humidity sensor and gas sensor.
4. Intelligent biochemistry incubator according to claim 3, it is characterised in that the gas sensor includes gas sensing
Device shell and microprocessor；Detection air chamber, multiple oscillators, frequency counter and difference are provided with the gas sensor shell
Frequency meter；The detection air chamber is provided with air inlet, and contrast crystal oscillator and target crystal oscillator are provided with detection air chamber, contrasts crystal oscillator and mesh
Mark crystal oscillator is electrically connected with corresponding oscillator and frequency counter respectively, and the two ends of the beat-frequency meter are connected to frequency meter
Number device, the 3rd end of the beat-frequency meter electrically connects with microprocessor；The target crystal oscillator surface has air-sensitive coating, the air-sensitive
Coating is nano polyaniline-activated carbon coating.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710032118.6A CN106701562A (en) | 2017-01-17 | 2017-01-17 | Intelligent biochemical incubator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710032118.6A CN106701562A (en) | 2017-01-17 | 2017-01-17 | Intelligent biochemical incubator |
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| Publication Number | Publication Date |
|---|---|
| CN106701562A true CN106701562A (en) | 2017-05-24 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201710032118.6A Pending CN106701562A (en) | 2017-01-17 | 2017-01-17 | Intelligent biochemical incubator |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102590008A (en) * | 2012-03-08 | 2012-07-18 | 中国科学院合肥物质科学研究院 | Quartz crystal micro-balance trace ammonia gas detection device |
| CN203174101U (en) * | 2013-02-18 | 2013-09-04 | 成都神鹤药业有限责任公司 | Intelligent biochemical culture box |
| CN204981842U (en) * | 2015-08-31 | 2016-01-20 | 重庆申高生化制药股份有限公司 | Biochemical cultivation case |
| CN205313564U (en) * | 2015-12-30 | 2016-06-15 | 恒盛高科（天津）生物技术有限公司 | Biochemical incubator of biological assay |
| CN205528807U (en) * | 2016-03-10 | 2016-08-31 | 王纪伟 | Incubator with network on -line monitoring |
-
2017
- 2017-01-17 CN CN201710032118.6A patent/CN106701562A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102590008A (en) * | 2012-03-08 | 2012-07-18 | 中国科学院合肥物质科学研究院 | Quartz crystal micro-balance trace ammonia gas detection device |
| CN203174101U (en) * | 2013-02-18 | 2013-09-04 | 成都神鹤药业有限责任公司 | Intelligent biochemical culture box |
| CN204981842U (en) * | 2015-08-31 | 2016-01-20 | 重庆申高生化制药股份有限公司 | Biochemical cultivation case |
| CN205313564U (en) * | 2015-12-30 | 2016-06-15 | 恒盛高科（天津）生物技术有限公司 | Biochemical incubator of biological assay |
| CN205528807U (en) * | 2016-03-10 | 2016-08-31 | 王纪伟 | Incubator with network on -line monitoring |
Non-Patent Citations (1)
| Title |
|---|
| 周文 等: "聚苯胺-多壁碳纳米管薄膜SAW N02传感器", 《传感器与微系统》 * |
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Application publication date: 20170524 |
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