CN106698630A - Preparation method of mariculture heavy metal pollution treating agent - Google Patents
Preparation method of mariculture heavy metal pollution treating agent Download PDFInfo
- Publication number
- CN106698630A CN106698630A CN201611142167.7A CN201611142167A CN106698630A CN 106698630 A CN106698630 A CN 106698630A CN 201611142167 A CN201611142167 A CN 201611142167A CN 106698630 A CN106698630 A CN 106698630A
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- Prior art keywords
- heavy metal
- preparation
- metal pollution
- sea
- farming
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Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/58—Treatment of water, waste water, or sewage by removing specified dissolved compounds
- C02F1/62—Heavy metal compounds
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/08—Seawater, e.g. for desalination
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/04—Disinfection
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- Life Sciences & Earth Sciences (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Processing Of Solid Wastes (AREA)
Abstract
The invention discloses a preparation method of a mariculture heavy metal pollution treating agent. The preparation method comprises steps as follows: step 1, seafood waste is processed into powder, and after water is added to the powder, a mixed solution is subjected to ultrasonic and irradiation treatment; step 2, protease is added to the mixed solution for high-pressure enzymolysis, then the solution is subjected to enzyme deactivation in water bath at the temperature of 100-120 DEG , centrifuged and filtered, a filtrate is obtained and subjected to reduced-pressure concentration to reach 1/2 of the original volume, and the mariculture heavy metal pollution treating agent is obtained. According to the preparation method, the seafood waste is utilized for preparing the treating agent, protein in the seafood waste can be effectively extracted through ultrasonic and irradiation treatment and taken as an active substance of the treating agent, the protein in the seafood waste can form small-molecule amino acid after protease treatment, an amino acid chelating agent is formed by the small-molecule amino acid and citric acid and can be bound with heavy metal in water to sink the heavy metal into the pond, and culture water quality is improved.
Description
Technical field
The present invention relates to water pollution processing technology field, and in particular to a kind of system of sea-farming heavy metal pollution inorganic agent
Make method.
Background technology
Marine sediment, refers to the pollution that some heavy metals are caused through various approach into ocean.Due to
The pollution that heavy metal is imported ocean and caused by mankind's activity.The heavy metal element of current Marine Pollution mainly have mercury, cadmium, lead,
Zinc, chromium, copper etc..Artificial source is mainly trade effluent, the discharge of mine wastewater and the loss of heavy metal agricultural chemicals, and coal and oil exist
The heavy metal discharged in burning enters ocean through the carrying of air.According to estimates, the whole world is entered due to mineral combustion every year
Entering the mercury in ocean has more than 3000 tons.Additionally, mercurous slag and ore pulp, are also released into ocean by a part of mercury.Thus, the whole world
The annual mercury entered in ocean by mankind's activity is up to 10,000 tons or so, and the annual production with nowadays world's mercury is suitable.Since 1924
Since year begins to use lead tetraethide as RONA, the concentration of lead hastily increases in air.By Atmospheric Transportation
Lead is the important channel of Marine Pollution, and lead, zinc, cadmium, mercury and the selenium for bringing open big midocean into through aerosol are input into total amount compared with land
More than 50%.Because the heavy metal pollution of seawater is increasingly serious, the cultivation to seawater produces serious threat, and sea can be caused for a long time
Heavy metals exceeding standard in water material culture body;Current Ecological Disposal agent has the chelating agent that single use feather hydrolysis albumen is made, system
It is long to make the method time, complex manufacturing technology.
The content of the invention
Seafood heel is seafood by remaining head pin waste material after processing, these waste materials generally as garbage disposal, easily
Cause environmental pollution, particularly air pollution.
One object of the present invention provides a kind of preparation method of sea-farming heavy metal pollution inorganic agent, using seafood pin
Material makes inorganic agent, not only acts as twice laid reduction cost of material, also acts as the effect of environmental protection.
The present invention provide technical scheme be:
A kind of preparation method of sea-farming heavy metal pollution inorganic agent, the preparation method includes:
Step 1, seafood heel is cleaned up after, be placed in it is subzero 20-30 DEG C freezing 3-6 hour after, using liquid nitrogen crushing,
Cross 100 mesh sieve and obtain seafood powder powder, add water, be placed in ultrasonic wave after 0.5-2 hours, then be placed into 5-10 in irradiation field
Minute;The irradiation dose of irradiation field is 20-25 kilocuries.
Step 2, by with seafood powder weight ratio be 50: 1 add protease, be placed in autoclave, add citric acid
PH is adjusted to 3-5, temperature is 45-65 DEG C, and after stirring 2-4 hours, then it is the 100-120 DEG C of enzyme that goes out to place temperature in a water bath,
Filtrate is filtrated to get after centrifugation, original volume 1/2 is concentrated under reduced pressure into, the sea-farming heavy metal pollution inorganic agent is obtained final product.
Preferably, the water addition in step 1 is heavy with seafood powder etc..
Preferably, the working frequency of ultrasonic wave is 100-400 hertz in step 1.
Preferably, the protease includes trypsase, papain, and 1: 1 mixing is compared with weight.
Preferably, it is placed in be placed in high-pressure pulse electric after addition protease in step 2 and is placed into height again in 20-30 minutes
In pressure reactor, wherein the electric-field intensity of the high-pressure pulse electric is 30-50kv, umber of pulse is 10;The pressure of autoclave
Power is 8-10Mpa;Shorten the time that protein digestion is decomposed under involving HIGH PRESSURE TREATMENT by pulse.
Preferably, photosynthetic bacteria 150CFU/ml is additionally added after being concentrated under reduced pressure in step 2;Increase photosynthetic bacteria except energy
Absorb heavy metal, moreover it is possible to which germ grows in suppressing water body.
Beneficial effects of the present invention are as follows:
The present invention makes inorganic agent using seafood heel, and involving radiation treatment treatment by ultrasound can effectively extract seafood pin
Protein in material as inorganic agent active material, the protein in seafood heel is by that can form small point after Protease Treatment
Sub- amino acid, chelating amino acids agent is formed with citric acid, can be combined with the heavy metal in water body, and by the heavy pool of heavy metal, purification is supported
Water quality is grown, the inventive method effectively utilizes seafood heel, plays twice laid, reduces the wasting of resources, while scientific disposal is improved
Inorganic agent efficiency.
Specific embodiment
With reference to specific embodiment, the present invention is described in further detail, to make those skilled in the art's reference say
Bright book word can be implemented according to this.
Embodiment 1
The preparation method of the present embodiment sea-farming heavy metal pollution inorganic agent includes:
Step 1, seafood heel is cleaned up after, be placed in it is subzero 20 DEG C freezing 3 hours after, using liquid nitrogen crush, cross 100
Mesh sieve obtains seafood powder powder, adds water, is placed in ultrasonic wave after 0.5 hour, then be placed into 5 minutes in irradiation field;Irradiation
The irradiation dose of field is 20-25 kilocuries.
Step 2, by with seafood powder weight ratio be 50: 1 add protease, be placed in autoclave, add citric acid
PH is adjusted to 3, temperature is 45 DEG C, after stirring 2 hours, then it is 100 DEG C of enzymes that go out to place temperature in a water bath, is filtered after centrifugation
To filtrate, original volume 1/2 is concentrated under reduced pressure into, obtains final product the sea-farming heavy metal pollution inorganic agent.
Embodiment 2
The preparation method of the present embodiment sea-farming heavy metal pollution inorganic agent includes:
Step 1, seafood heel is cleaned up after, be placed in it is subzero 30 DEG C freezing 6 hours after, using liquid nitrogen crush, cross 100
Mesh sieve obtains seafood powder powder, and the water of the weight such as addition and seafood powder is placed in ultrasonic wave after 2 hours, then be placed into irradiation
10 minutes in;The irradiation dose of irradiation field is 25 kilocuries, and the working frequency of ultrasonic wave is 400 hertz.
Step 2, by with seafood powder weight ratio be 50: 1 add protease, the protease include trypsase, pawpaw
Protease, 1: 1 mixing is compared with weight, is placed in high-pressure pulse electric in placing and placing within 30 minutes autoclave again, adds lemon
Lemon acid adjusts to 5 PH, and temperature is 65 DEG C, and after stirring 4 hours, then it is 120 DEG C of enzymes that go out, mistake after centrifugation to place temperature in a water bath
Filter obtains filtrate, is concentrated under reduced pressure into original volume 1/2, and photosynthetic bacteria 150CFU/ml is obtained final product at the sea-farming heavy metal pollution
Reason agent;The electric-field intensity of wherein described high-pressure pulse electric is 50kv, and umber of pulse is 10;The pressure of autoclave is 10Mpa;
Shorten the time that protein digestion is decomposed under involving HIGH PRESSURE TREATMENT by pulse.
Although embodiment of the present invention is disclosed as above, it is not restricted to listed in specification and implementation method
With, it can be applied to various suitable the field of the invention completely, for those skilled in the art, can be easily
Other modification is realized, therefore under the universal limited without departing substantially from claim and equivalency range, the present invention is not limited
In specific details and shown here as the embodiment with description.
Claims (6)
1. a kind of preparation method of sea-farming heavy metal pollution inorganic agent, it is characterised in that the preparation method includes:
Step 1, seafood heel is cleaned up after, be placed in it is subzero 20-30 DEG C freezing 3-6 hour after, using liquid nitrogen crushing, mistake
100 mesh sieve obtain seafood powder powder, add water, are placed in ultrasonic wave after 0.5-2 hours, then are placed into 5-10 points in irradiation field
Clock;The irradiation dose of irradiation field is 20-25 kilocuries.
Step 2, it is to add protease at 50: 1 by with seafood powder weight ratio, is placed in autoclave, adds citric acid by PH
To 3-5, temperature is 45-65 DEG C, and after stirring 2-4 hours, then it is the 100-120 DEG C of enzyme that goes out to place temperature in a water bath, centrifugation for regulation
After be filtrated to get filtrate, be concentrated under reduced pressure into original volume 1/2, obtain final product the sea-farming heavy metal pollution inorganic agent.
2. the preparation method of sea-farming heavy metal pollution inorganic agent as claimed in claim 1, it is characterised in that in step 1
The weight such as water addition and seafood powder.
3. the preparation method of sea-farming heavy metal pollution inorganic agent as claimed in claim 1, it is characterised in that in step 1
The working frequency of ultrasonic wave is 100-400 hertz.
4. the preparation method of sea-farming heavy metal pollution inorganic agent as claimed in claim 1, it is characterised in that the albumen
Enzyme includes trypsase, papain, and 1: 1 mixing is compared with weight.
5. the preparation method of sea-farming heavy metal pollution inorganic agent as claimed in claim 1, it is characterised in that in step 2
It is placed in be placed in high-pressure pulse electric after addition protease and is placed into autoclave again for 20-30 minutes, wherein the high pressure
The electric-field intensity of impulse electric field is 30-50kv, and umber of pulse is 10;The pressure of autoclave is 8-10Mpa.
6. the preparation method of sea-farming heavy metal pollution inorganic agent as claimed in claim 1, it is characterised in that in step 2
Photosynthetic bacteria 150CFU/ml is additionally added after concentrated under reduced pressure.
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2019119998A1 (en) * | 2017-12-22 | 2019-06-27 | 浙江省海洋开发研究院 | Protein-derived, heavy metal removal agent suitable for use in mussels and preparation method therefor |
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CN101487038A (en) * | 2008-12-05 | 2009-07-22 | 江苏大学 | Preparation and use of maize yellow powder protein hydrolysate with hypotensive function |
CN101701241A (en) * | 2009-11-05 | 2010-05-05 | 山东好当家海洋发展股份有限公司 | Preparation method of scallop active peptide |
CN102277404A (en) * | 2011-08-05 | 2011-12-14 | 北京市水产科学研究所 | Method for preparing fish protein by using waste from fish processing |
CN104386827A (en) * | 2014-10-31 | 2015-03-04 | 安徽华亿农牧科技发展有限公司 | Environment-friendly water purifier, preparation and using methods thereof |
CN104938604A (en) * | 2015-06-10 | 2015-09-30 | 台建祥 | Comprehensive aquatic product processing leftover utilization method |
DE202016102902U1 (en) * | 2016-05-31 | 2016-06-17 | Lien-Ta Chen | Device for obtaining a skin protein from Esox lucius |
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2016
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CN101487038A (en) * | 2008-12-05 | 2009-07-22 | 江苏大学 | Preparation and use of maize yellow powder protein hydrolysate with hypotensive function |
CN101701241A (en) * | 2009-11-05 | 2010-05-05 | 山东好当家海洋发展股份有限公司 | Preparation method of scallop active peptide |
CN102277404A (en) * | 2011-08-05 | 2011-12-14 | 北京市水产科学研究所 | Method for preparing fish protein by using waste from fish processing |
CN104386827A (en) * | 2014-10-31 | 2015-03-04 | 安徽华亿农牧科技发展有限公司 | Environment-friendly water purifier, preparation and using methods thereof |
CN104938604A (en) * | 2015-06-10 | 2015-09-30 | 台建祥 | Comprehensive aquatic product processing leftover utilization method |
DE202016102902U1 (en) * | 2016-05-31 | 2016-06-17 | Lien-Ta Chen | Device for obtaining a skin protein from Esox lucius |
CN105948275A (en) * | 2016-06-29 | 2016-09-21 | 合肥天翔环境工程有限公司 | Sewage treatment agent and preparation method thereof |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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WO2019119998A1 (en) * | 2017-12-22 | 2019-06-27 | 浙江省海洋开发研究院 | Protein-derived, heavy metal removal agent suitable for use in mussels and preparation method therefor |
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CB03 | Change of inventor or designer information | ||
CB03 | Change of inventor or designer information |
Inventor after: Wang Weijian Inventor after: Xie Meijuan Inventor before: Zhong Shuming |
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WD01 | Invention patent application deemed withdrawn after publication | ||
WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20170524 |