CN106693041A - Asymmetric wettable chitosan/starch compound sponge and preparation method thereof - Google Patents
Asymmetric wettable chitosan/starch compound sponge and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/04—Surgical adhesives or cements; Adhesives for colostomy devices containing macromolecular materials
- A61L24/043—Mixtures of macromolecular materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
- A61L24/0036—Porous materials, e.g. foams or sponges
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L24/00—Surgical adhesives or cements; Adhesives for colostomy devices
- A61L24/001—Use of materials characterised by their function or physical properties
- A61L24/0042—Materials resorbable by the body
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
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Abstract
The invention discloses asymmetric wettable chitosan/starch compound sponge and a preparation method thereof. By using safe and non-toxic chitosan which is good in biocompatibility and can be biologically self-degraded as a primary raw material and adding non-toxic starch as well as an additive, porous compound sponge of chitosan and starch is obtained by virtue of a simple freeze-drying method, and then the chitosan/starch compound sponge with asymmetric wettability is obtained by surface modification by virtue of a modifier. According to the method disclosed by the invention, a specific surface of a material needs not to be specifically modified, so that a sponge material, one side of which is lyophobic and the other side of which is hydrophilic, can be obtained, and the sponge has an important application prospect in the field of medical wound dressing and the like.
Description
Technical field
The invention belongs to technical field of function materials, and in particular to a kind of shitosan/starch with asymmetric wetability
Composite sponge and preparation method thereof.
Background technology
Asymmetric wettable surfaces are that the two sides of same material shows different wetabilitys, such as the hydrophobic another side table of one side
Reveal hydrophilic material.Asymmetric wetability boundary material as the one kind in special wetability material, because its have it is special
Performance is unidirectionally transported in fluid, microfluid system, water-oil separating, and the aspect such as wound dressing shows huge potential application valency
Value.Particularly in external application art of wound dressings, because the hydrophobic surface of asymmetric wetability material has automatically cleaning and antifouling work
With, can effectively stop the pollution and destruction to material such as alien bacteria and environmental liquids, and hydrophilic surface can absorb wound
Sepage, the environment of moistening is provided for the growth of cell, promotes wound healing.Which solve traditional dressing easy quilt in use
The unholiness liquid pollution such as alien bacteria, blood, so as to cause wound infection to influence the deficiency of wound healing.Therefore, preparing not
Symmetrical wetability material turns into nearest study hotspot.
Shen Liyan etc. (ACS Appl.Mater.Interfaces 2012,4,4476-4483) are using layer by layer from group
The method of dress prepares the polyethyleneimine-Ag with asymmetric wetability+With polyacrylic acid (PEI-Ag/PAA)20It is thin
Film, because the one side of the thin-film hydrophobic has automatic cleaning action, can stop the dirt to material such as alien bacteria and environmental liquids
Dye and destruction, the releasable Ag of hydrophilic one side+The bacterium of wound is killed the effect for reaching antibacterial, therefore can be hindered as one kind
Mouth dressing.But this preparation technology is complex, it is necessary to from special ground as masterplate, then be incited somebody to action under the conditions of specific pH
PEI-Ag and PAA LBL self-assemblies more than 20 times, finally carrying out silylating reagent in its coarse one side can just prepare the membrane material
Material, is not easy to large-scale production.LiuYuyang etc. (Langmuir, 2012,28,17426-17434) is using knife coating in cotton
One side coat one layer of fluoropolymer, the hydrophilic asymmetric wetability cotton in the super-hydrophobic one side of one side is prepared after drying.
Although the preparation method is simple, need strict control coated weight, coating thickness and penetration depth to obtain having one side super thin
Water hydrophilic cotton on one side, in addition the asymmetric wetability cotton surface contain fluoropolymer, can opposite for wound dressing
Object causes certain toxic and side effect.Therefore, the simple asymmetric wetability material of environment-friendly and preparation method is further explored
Material has important actual application value.
The content of the invention
The purpose of the present invention is directed to the deficiencies in the prior art, there is provided a kind of shell with asymmetric wetability gathers
Sugar/starch composite sponge and preparation method thereof, the raw material sources of use are abundant, safety non-toxic, and the preparation method being related to is simple, easily
Operation, gained compound sponges material is hydrophilic while hydrophobic, and with good asymmetric wetability, and gas permeability is high, softness
Property is good, with important actual application value.
To achieve the above object, the technical solution adopted by the present invention is：
A kind of preparation method of asymmetric wetability shitosan/starch composite sponge, comprises the following steps：
1) by shitosan, cornstarch in stirring and dissolving in acetum, prepare shitosan/starch acetate solution, will
This solution is poured into mould, and after standing froth breaking, then precooling carries out freeze-drying process, then by gained to freezing real completely
Product removes the acetic acid of residual in being immersed in alkali lye, then is rinsed repeatedly with deionized water to neutrality, will finally be washed till the product of neutrality
Thing freeze-dried treatment again, obtains shitosan/starch composite sponge body；
2) step 1) gained shitosan/starch composite sponge body be soaked in the ethanol solution of modifying agent, take out drying
After obtain final product the asymmetric wetability shitosan/starch composite sponge.
By such scheme, step 1) described in stirring and dissolving temperature be 55~60 DEG C.
By such scheme, the mass ratio of the shitosan and starch is 1:(0.5~2).
Preferably, the mass ratio of the shitosan and starch is 1:(0.5~1).
As stated above, the concentration of the acetum is 1~2%.
By such scheme, the deacetylation of the shitosan is 70~100%, and viscosity is 100~200mPas.
By such scheme, the ratio between the quality of the shitosan and the volume of acetum are 0.01-0.03g/mL.
In such scheme, the temperature conditionss of the precooling are less than -18 DEG C, and the time is 8~12h.
In such scheme, the process conditions of the freeze-drying process are：Freeze drier condenser temperature≤- 40 DEG C, very
Reciprocal of duty cycle≤10Pa, sublimation drying is 16-20h.
In such scheme, the modifying agent is the one kind in stearic acid, behenic acid, arachidic acid；The ethanol of the modifying agent
The concentration of modifying agent is 10~50mmol/L in solution.
In such scheme, soaking temperature described in step (2) is room temperature, and soak time is 5~60min.
According to asymmetric wetability shitosan/starch composite sponge prepared by such scheme, it is hydrophilic while hydrophobic,
Wherein hydrophobic surface has automatically cleaning and antifouling property, can stop bacterial penetration and adhesion, prevent external unholiness liquid to material
Pollution, hydrophilic surface can absorb wound exudate, moisturizing and promote wound healing, can be applied to wound dressing；It has preferably
Gas permeability, water-retaining property, biocompatibility and biological degradability, above have wide prospect in the application of medical science wound dressing.
The formation mechenism of asymmetric wetability shitosan/starch composite sponge of the present invention is：The present invention is with shitosan
It is raw material, starch is additive, the two is stirred at a certain temperature and is dissolved in dilute acid soln, through vacuum freeze drying and surface
Modification obtains asymmetric wetability shitosan/compound porous sponge of starch；In this preparation process, shitosan is fully soluble in dilute
Starch meeting partial gelatinization in acid solution, starch can be settled under gravity during standing and precooling, gather shell
Sugar shows a kind of gradient distribution along gravity direction in the solution so that the shitosan of dried sponge top and bottom
Skewness；Shitosan belongs to a kind of cationic alkaline polysaccharide simultaneously, and it exists quiet and the carboxyl of LCFA between
Electric attraction, when surface modification is carried out to sponge with LCFA, due to interaction force therebetween, sponge top and
The amount of the LCFA of bottom can skewness (in the few state in many bottoms in top), along with the hygroscopicity and just of starch
The capillary effect that the asymmetric loose structure of anti-two sides (composite sponge upper and lower part) (hole on top is significantly more than bottom) causes
Should, so that gained composite sponge shows the hydrophilic asymmetric wetting in the hydrophobic bottom in top.
The present invention using safety non-toxic, good biocompatibility and can the biological shitosan from degraded as primary raw material, plus
Enter same nontoxic starch as additive, the compounding sea of porous shitosan and starch is obtained by simple freeze-drying
Silk floss, then the modified dose of step of surface modification one prepares the shitosan with asymmetric wetability/starch composite sponge, the method
Specially it is modified without the certain surface to material, so that it may hydrophilic sponge material while hydrophobic is obtained, in medical wound
The fields such as dressing have important application prospect.
Beneficial effects of the present invention are:
1) the asymmetric wetability shitosan/starch composite sponge preparation method prepared by the present invention is simple, production process
Environmental protection, it is with short production cycle, specially it is modified without the certain surface to material, so that it may obtain hydrophilic while hydrophobic
Compound sponges material.
2) present invention prepared by asymmetric wetability shitosan/starch composite sponge used shitosan, starch and
The raw material sources such as aliphatic acid are wide, biological safety is high, and human body cell is had no toxic side effect, and shitosan and starch are used as a kind of natural
Not only raw material sources enrich macromolecular compound, and biodegradable, do not easily cause secondary pollution.
3) the asymmetric wetability shitosan/starch composite sponge prepared by the present invention, its gas permeability is high, soft moisture retention
It is good, cell dehydration can be prevented, promote cell growth be also less prone to surface of a wound adhesion, wound healing can be effectively facilitated.
Brief description of the drawings
Fig. 1 is that the SEM of the asymmetric wetability shitosan/starch composite sponge of gained of embodiment 1 schemes, (a) hydrophobic layer surface,
(b) hydrophilic layer surface；
Fig. 2 is that the water droplet of rhodamine B dyeing is dredged in the asymmetric wetability shitosan/starch composite sponge of gained of embodiment 1
The photo of the water surface and hydrophilic surface, (a) hydrophobic layer surface, (b) hydrophilic layer surface；
Fig. 3 is that the asymmetric wetability shitosan of the gained of embodiment 1/starch composite sponge hydrophobic surface water droplet rolls photo；
Fig. 4 is the water contact of the asymmetric wetability shitosan/starch composite sponge hydrophobic surface of the gained of embodiment 1 and hydrophilic surface
Angle state diagram, (a) hydrophobic layer surface, (b) hydrophilic layer surface；
Fig. 5 is the water contact angle state diagram of the chitosan multi-porous sponge upper and lower surface of gained of comparative example 1, (a) upper table
Face, (b) lower surface；
Fig. 6 is the asymmetric wetability shitosan/starch composite sponge antibacterium permeability experiment of gained of embodiment 1, on (a)
Surface, (b) bottom bacterium colony.
Specific embodiment
The present invention is described further with reference to embodiment and accompanying drawing, the scope of protection of present invention is not limited to
In the scope of embodiment statement.
Embodiment 1
A kind of asymmetric wetability shitosan/starch composite sponge, its preparation method comprises the following steps：
1) by shitosan (deacetylation is 95%, and viscosity is 200mPa.s), cornstarch by 1:1 mass ratio is added to
In the acetum of 1wt%, magnetic agitation to shitosan and cornstarch is completely dissolved at a temperature of 60 DEG C, and preparation shitosan/
Starch acetate solution (the ratio between the quality of shitosan and the volume of acetum are 0.02g/mL), this solution is poured into mould,
After standing froth breaking, in -20 DEG C of precooling 10h, it is then placed in carrying out freeze-drying process in vacuum freeze drier, wherein freezing
The parameter of drying machine is：- 55 DEG C of condenser temperature, vacuum 0.63Pa, cooling time is 16h, obtains shitosan/starch composite sponge
Body；Products therefrom is soaked in the NaOH solution that concentration is 2wt% and removes the acetic acid of residual, and rinsed repeatedly with deionized water
To neutrality, the product that will be washed till neutrality carries out freeze-drying process, obtains shitosan/starch composite sponge body again；
2) step 1) gained shitosan/starch composite sponge body be soaked in stearic acid concentration be 20mmol/L stearic acid
Ethanol solution in, after 30min take out, the asymmetric wetability shitosan/starch composite sponge is obtained final product after drying.
Fig. 1 is the surface topography map (SEM figures) of the present embodiment asymmetric wetability shitosan/starch composite sponge of gained.
It can be seen that the hydrophobic surface and hydrophilic surface of sponge are loose structure, the hole of hydrophilic surface is significantly more than hydrophobic surface.
Fig. 2 is the water droplet for dyeing rhodamine B in asymmetric wetability shitosan/starch composite sponge obtained by the present embodiment
The state diagram of hydrophobic surface and hydrophilic surface, it can be seen that water droplet is in hydrophobic layer surface almost globulate, and inclines sample water
Drop can be rolled away quickly (see Fig. 3), the water droplet of hydrophilic surface shitosan/starch compounding sea obtained by inside whole inspiration sponges, illustrate
Silk floss has preferable hydrophobic performance for one side, and another side has the asymmetric wetability material of hydrophilicity.
Fig. 4 is water droplet asymmetric wetability shitosan/starch composite sponge hydrophobic surface and hydrophilic surface in the present embodiment
Contact angle photo, the contact angle of its hydrophobic surface is 150 °, and hydrophilic surface is 0 °, shows that the shitosan/starch composite sponge shows
Simultaneously super-hydrophobic and a face Superhydrophilic asymmetric wetability.This characteristic is conducive to the sponge when for wound dressing,
While wound exudate is absorbed, can prevent the unholiness liquid in the external world from infiltering wound again.
Embodiment 2
A kind of asymmetric wetability shitosan/starch composite sponge, its preparation method comprises the following steps：
1) by shitosan (deacetylation is 85%, and viscosity is 150mPa.s), cornstarch by 1:0.5 mass ratio is added
To in the acetum of 1wt%, magnetic agitation to shitosan and cornstarch is completely dissolved at a temperature of 60 DEG C, prepares shell and gathers
Sugar/starch acetate solution (the ratio between the quality of shitosan and the volume of acetum are 0.02g/mL), mould is poured into by this solution
In, after standing froth breaking, in -20 DEG C of precooling 11h, it is then placed in carrying out freeze-drying process in vacuum freeze drier, wherein
The parameter of freeze drier is：- 50 DEG C of condenser temperature, vacuum 5Pa, cooling time is 20h, obtains shitosan/starch compounding sea
Continuous body；Products therefrom is soaked in the NaOH solution that concentration is 2wt% and removes the acetic acid of residual, and rushed repeatedly with deionized water
Neutrality is washed till, the product freeze-dried treatment again of neutrality will be washed till, obtain shitosan/starch composite sponge body；
2) step 1) gained shitosan/starch composite sponge body be soaked in concentration be 10mmol/L stearic ethanol
In solution, taken out after 50min, the asymmetric wetability shitosan/starch composite sponge is obtained final product after drying.
Embodiment 3
A kind of asymmetric wetability shitosan/starch composite sponge, its preparation method comprises the following steps：
1) by shitosan (deacetylation is 70%, and viscosity is 200mPa.s), cornstarch by 1:2 mass ratio is added to
In the acetum of 1wt%, magnetic agitation to shitosan and cornstarch is completely dissolved at a temperature of 60 DEG C, and preparation shitosan/
Starch acetate solution (the ratio between the quality of shitosan and the volume of acetum are 0.01g/mL), this solution is poured into mould,
After standing froth breaking, in -18 DEG C of precooling 10h, it is then placed in carrying out freeze-drying process in vacuum freeze drier, wherein freezing
The parameter of drying machine is：- 45 DEG C of condenser temperature, vacuum 8Pa, cooling time is 16h, obtains shitosan/starch composite sponge body；
By products therefrom be soaked in concentration be 2wt% NaOH solution in remove the acetic acid of residual, and rinsed repeatedly with deionized water to
Neutrality, will be washed till the product freeze-dried treatment again of neutrality, obtain shitosan/starch composite sponge body；
2) step 1) gained shitosan/starch composite sponge body be soaked in concentration be 50mmol/L arachidic acid ethanol
In solution, taken out after 5min, the asymmetric wetability shitosan/starch composite sponge is obtained final product after drying.
Embodiment 4
A kind of asymmetric wetability shitosan/starch composite sponge, its preparation method comprises the following steps：
1) by shitosan (deacetylation is 100%, and viscosity is 100mPa.s), cornstarch by 1:1 mass ratio is added
To in the acetum of 1wt%, magnetic agitation to shitosan and cornstarch is completely dissolved at a temperature of 60 DEG C, prepares shell and gathers
Sugar/starch acetate solution (the ratio between the quality of shitosan and the volume of acetum are 0.01g/mL), mould is poured into by this solution
In, after standing froth breaking, in -20 DEG C of precooling 10h, it is then placed in carrying out freeze-drying process in vacuum freeze drier, wherein
The parameter of freeze drier is：- 40 DEG C of condenser temperature, vacuum 10Pa, cooling time is 18h, obtains shitosan/starch compounding sea
Continuous body；Products therefrom is soaked in the NaOH solution that concentration is 2wt% and removes the acetic acid of residual, and rushed repeatedly with deionized water
Neutrality is washed till, the product freeze-dried treatment again of neutrality will be washed till, obtain shitosan/starch composite sponge body；
2) step 1) gained shitosan/starch composite sponge body be soaked in concentration be 20mmol/L arachidic acid ethanol
In solution, taken out after 60min, the asymmetric wetability shitosan/starch composite sponge is obtained final product after drying.
Embodiment 5
A kind of asymmetric wetability shitosan/starch composite sponge, its preparation method comprises the following steps：
1) by shitosan (deacetylation is 90%, and viscosity is 200mPa.s), cornstarch by 1:0.5 mass ratio is added
To in the acetum of 1wt%, magnetic agitation to shitosan and cornstarch is completely dissolved at a temperature of 60 DEG C, prepares shell and gathers
Sugar/starch acetate solution (the ratio between the quality of shitosan and the volume of acetum are 0.03g/mL), mould is poured into by this solution
In, after standing froth breaking, in -18 DEG C of precooling 12h, it is then placed in carrying out freeze-drying process in vacuum freeze drier, wherein
The parameter of freeze drier is：- 53 DEG C of condenser temperature, vacuum 1Pa, cooling time is 20h, obtains shitosan/starch compounding sea
Continuous body；Products therefrom is soaked in the NaOH solution that concentration is 2wt% and removes the acetic acid of residual, and rushed repeatedly with deionized water
Neutrality is washed till, the product freeze-dried treatment again of neutrality will be washed till, obtain shitosan/starch composite sponge body；
2) step 1) gained shitosan/starch composite sponge body be soaked in concentration be 30mmol/L behenic acid ethanol
In solution, taken out after 20min, the asymmetric wetability shitosan/starch composite sponge is obtained final product after drying.
Gas permeability and water absorbing properties are tested
Embodiment 1-3 products therefroms are carried out into water evaporation transmitance and water absorption rate test, its gas permeability and water suction is tested
Performance, test result is shown in Table 1.
Table 1
Test result shows that the asymmetric wetability shitosan/starch composite sponge of present invention gained has good breathing freely
Property and water absorbing properties.
Comparative example 1
A kind of chitosan multi-porous sponge, its preparation method is roughly the same with embodiment 1, and difference is to be not added with corn
Starch.
This comparative example chitosan multi-porous sponge of gained is carried out into surface wettability test, is comprised the following steps that：By the water of 3 μ L
The tow sides of chitosan sponge are added drop-wise to respectively, and the water contact angle of sponge surface is tested by contact angle measurement.
Experimental result is shown in Fig. 5, water droplet chitosan multi-porous sponge positive and negative obtained by this comparative example contact angle photo, its positive contact
Angle is 146 °, and reverse side contact angle is hydrophobic for 143 °, i.e. the sponge tow sides.Show with embodiment Comparative result, it is beautiful
The addition of rice starch has important effect to the shitosan/starch composite sponge for obtaining asymmetric wetability.
Comparative example 2
A kind of shitosan/starch composite sponge, its preparation method is roughly the same with embodiment 1, and difference is not add
Stiffened resin acid.
Antibacterium permeance property is tested
Asymmetric wetability shitosan/shallow lake prepared by the gained shitosan of comparative example 2/starch composite sponge and embodiment 1
Powder composite sponge carries out antibacterium permeance property test respectively, comprises the following steps that：By the asymmetric wetability shell of gained of embodiment 1
Glycan/starch composite sponge is hydrophobic to face up, and being laid in agar side by side with the gained shitosan of comparative example 2/starch composite sponge trains
Support on base, then (bacterial concentration is 10 to 200 μ L Escherichia coli bacteria liquids of each dropwise addition on every group of sample6CFU/mL, i.e., every milliliter
The colony counts of bacterium are 10 in bacterium solution6It is individual), observed after being placed 24 hours in 37 DEG C of incubators.Experimental result is shown in Fig. 6, figure
Bacterium solution on the asymmetric wetability shitosan/starch composite sponge hydrophobic surface of the gained of middle embodiment 1 still remains in surface and does not ooze
Enter inside, and the bacterium solution on the gained shitosan of comparative example 2/starch composite sponge surface has infiltered sample；Two groups of sponges are opened, it is real
Apply the asymmetric wetability shitosan/starch composite sponge bottom of the gained of example 1 and surrounding remain in that cleaning, there is no colony growth,
And the gained shitosan of comparative example 2/starch composite sponge bottom and surrounding then observe have large stretch of bacterium colony.Test result shows：
Asymmetric wetability shitosan/starch composite sponge prepared by the present invention can effectively prevent extraneous bacterium from infiltering inside sponge, have
There is preferable antibiont permeability.
The foregoing is only the preferred embodiment of the present invention, it is noted that come for one of ordinary skill in the art
Say, without departing from the concept of the premise of the invention, make some modifications and variations, these belong to protection model of the invention
Enclose.
Claims (10)
1. a kind of preparation method of asymmetric wetability shitosan/starch composite sponge, comprises the following steps：
1) by shitosan, cornstarch stirring and dissolving in acetum, shitosan/starch acetate solution is prepared, by this solution
Pour into mould, after standing froth breaking, then precooling carries out freeze-drying process to freezing real completely, then soaks products therefrom
Bubble removes the acetic acid of residual in alkali lye, then is rinsed repeatedly with deionized water to neutrality, will finally be washed till the product of neutrality again
Freeze-dried treatment, obtains shitosan/starch composite sponge body；
2) step 1) gained shitosan/starch composite sponge body be soaked in the ethanol solution of modifying agent, take out drying after i.e.
Obtain the asymmetric wetability shitosan/starch composite sponge.
2. preparation method according to claim 1, it is characterised in that the mass ratio of the shitosan and starch is 1:(0.5
~2).
3. preparation method according to claim 1, it is characterised in that the mass ratio of the shitosan and starch is 1:(0.5
~1).
4. preparation method according to claim 1, it is characterised in that the quality of the shitosan and the volume of acetum
The ratio between be 0.01-0.03g/mL.
5. preparation method according to claim 1, it is characterised in that the temperature conditionss of the precooling are less than -18 DEG C,
Time is 8-12h.
6. preparation method according to claim 1, it is characterised in that the process conditions of the freeze-drying process are：It is cold
Lyophilizer condenser temperature≤- 40 DEG C, vacuum≤10Pa, sublimation drying is 16-20h.
7. preparation method according to claim 1, it is characterised in that the modifying agent is stearic acid, behenic acid, arachidic acid
In one kind.
8. preparation method according to claim 1, it is characterised in that modifying agent is dense in the ethanol solution of the modifying agent
It is 10~50mmol/L to spend.
9. preparation method according to claim 1, it is characterised in that step 2) described in soaking temperature be room temperature, immersion
Time is 5~60min.
10. asymmetric wetability shitosan/starch composite sponge that prepared by preparation method described in any one of claim 1~9.
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| CN109731121A (en) * | 2018-12-31 | 2019-05-10 | 武汉工程大学 | A kind of preparation method of the cellulose containing mesoporous silicon oxide and chitosan combine dressing |
| CN112358659A (en) * | 2020-11-26 | 2021-02-12 | 南京信息工程大学 | Polysaccharide nano-fibril reinforced flame-retardant starch composite foam and preparation method thereof |
| CN112569398A (en) * | 2020-12-18 | 2021-03-30 | 南京鼓楼医院 | Anisotropic wound dressing and preparation method thereof |
| CN112618780A (en) * | 2020-12-07 | 2021-04-09 | 徐敏 | Hemostatic sponge for digestive department and preparation method thereof |
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| CN107693427A (en) * | 2017-11-01 | 2018-02-16 | 湖北工程学院 | A kind of anti-oxidant beauty mask and preparation method thereof |
| CN107693427B (en) * | 2017-11-01 | 2020-10-30 | 湖北工程学院 | Antioxidant beauty mask and preparation method thereof |
| CN109731121A (en) * | 2018-12-31 | 2019-05-10 | 武汉工程大学 | A kind of preparation method of the cellulose containing mesoporous silicon oxide and chitosan combine dressing |
| CN109731121B (en) * | 2018-12-31 | 2021-11-02 | 武汉工程大学 | Preparation method of cellulose and chitosan composite dressing containing mesoporous silica |
| CN112358659A (en) * | 2020-11-26 | 2021-02-12 | 南京信息工程大学 | Polysaccharide nano-fibril reinforced flame-retardant starch composite foam and preparation method thereof |
| CN112618780A (en) * | 2020-12-07 | 2021-04-09 | 徐敏 | Hemostatic sponge for digestive department and preparation method thereof |
| CN112569398A (en) * | 2020-12-18 | 2021-03-30 | 南京鼓楼医院 | Anisotropic wound dressing and preparation method thereof |
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