CN106680203A - Online microalgae automatic sampling optical measuring system - Google Patents
Online microalgae automatic sampling optical measuring system Download PDFInfo
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- CN106680203A CN106680203A CN201510762836.XA CN201510762836A CN106680203A CN 106680203 A CN106680203 A CN 106680203A CN 201510762836 A CN201510762836 A CN 201510762836A CN 106680203 A CN106680203 A CN 106680203A
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- sampling pond
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- microalgae
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/15—Preventing contamination of the components of the optical system or obstruction of the light path
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/03—Cuvette constructions
- G01N2021/0367—Supports of cells, e.g. pivotable
- G01N2021/0375—Slidable cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N21/15—Preventing contamination of the components of the optical system or obstruction of the light path
- G01N2021/152—Scraping; Brushing; Moving band
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/04—Batch operation; multisample devices
- G01N2201/0407—Batch operation; multisample devices with multiple optical units, e.g. one per sample
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Optical Measuring Cells (AREA)
Abstract
The application discloses an online microalgae automatic sampling optical measuring system. The system is composed of a frame, a motor sliding table unit, a connecting piece unit, a position sensor pair, a positioning baffle plate, a sampling pond, a measuring module unit, a measuring tube positioning card pair and the like, wherein the frame is made from an opaque material; the motor sliding table unit sucks or pumps a sample into the sampling pond by connecting a piston in the sampling pond through a connecting assembly, and meanwhile, the position sensor pair and the positioning baffle plate control the distance and position of piston movement and provide a signal that the measuring module unit starts to measure; and the measuring module unit can be combined and matched by various different optical measuring modules. In a microalgae culture process, optical parameters of microalgae are continuously measured online by matching different optical measuring modules. As the machine can be matched with a plurality of modules at the same time, a plurality of data can be measured at the same time, and meanwhile, the size of the microalgae online culture and detection system is reduced, and the system is low in cost, easy to connect and integrate and suitable for microalgae online culture system.
Description
Technical field
This patent is related to a kind of online microalgae automatic sampling optical measuring system, and this device can be used for microdisk electrode
During various optical parametrics change, the physiological statuss and upgrowth situation of microalgae can be embodied, be that microalgae is online
Critically important measuring apparatus in culture.
Background technology
At present the online optical measuring apparatus of microalgae mostly are single equipment or immersion cell, when the multiple optics of needs
In-situ measurement equipment is bulky during measurement parameter, and integrated difficulty is high, and equipment cost is high, is unfavorable for microalgae
Application in scale evaluation.Immersion optic probe is during high-concentration culturing due to the near-wall air curtain of microalgae
And the reason for microorganism pollution, the shelter for stopping probe optical face can be formed, this can substantially reduce immersion
The accuracy of optic probe.This patent can simultaneously measure various optical parametrics by once sampling, greatly improve
Integrated level, reduces equipment volume while substantially reducing cost；Due to being provided with piston in this patent sampling pond,
Piston is playing a part of pipette samples and is releasing the effect that cleaning sampling pond inwall is also acted outside sample, makes
Microalgae and microorganism are not easy to attach on the inwall for sampling pond, make sampling pond remain clean state, carry
The accuracy of high optical measurement, makes the online accurately optical measurement of the long-time during high concentration microdisk electrode
It is possibly realized.
To being not suitable as the problems such as the pollution of probe during at present majority instrument is cultivated because of volume, price, long-time
The on-line measurement of microalgae large-scale culture, this patent solves problem above simultaneously, can be in high concentration microalgae
In incubation, online long-time accurately measures various optical parametrics, provides for microalgae large-scale culture process
Mass data.
The content of the invention
In order to reduce during microdisk electrode because of microalgae and the adherent impact caused to optical measurement of microorganism, this
By the way that sampling cock and piston are arranged in sampling pond by material systems such as the softer rubber of quality or silica gel in patent
Into, cleaning sampling inside pipe wall is can be very good along in sampling pond inwall sliding process in piston, it is that optics is surveyed
Amount provides good condition；In order to reduce the volume of the online Systems for optical inspection of whole microalgae, this patent design
It is whole while increased the optical parametric quantity of measurement can simultaneously to install various optical measurement modules
The constancy of volume of equipment；This patent measures multiple optical parametrics while only needing to a sample tap simultaneously, one
Data-interface, very convenient system it is integrated, while greatly reducing cost.
Online microalgae automatic sampling optical measuring system, including framework, motor slide unit group, connector group, position
Sensor is to, positioning baffle, sampling pond, measurement module group, measurement pipe locator card pair.
The framework is made up of material that is opaque and having certain machinery strong, and framework plays support internal structure and screening
The effect of gear extraneous light；By including frame base, framework dividing plate, framework enclosing cover composition.
The sampling pond is constituted by pond outer tube, sampling pond piston is sampled, wherein sampling pond outer tube is by glass, quartz
Or the good material of light transmission such as plastics is made；Sample piston head of the pond piston by one end, the piston at middle part
Connecting rod, the cylindrical projections composition of the other end, wherein piston head is by the softer rubber of quality or silica gel etc.
Material is made, and piston brace rod is made up of plastics or metal material, and cylindrical projections are made up of plastics or metal；
The piston head of soft texture can clean sampling pond outer tube when sampling and being slided in the outer tube of pond while sealing is ensured
Inwall and do not damage sampling pond outer tube inwall, provide good condition for optical measurement.
The motor slide unit group is by slid platform base, motor, drive screw, slide block composition；Motor and biography
Dynamic screw rod is connected by shaft coupling, and motor rotation drives drive screw to rotate, by slide block inside and screw rod
The thread belt movable slider for matching is moved along the slid platform base that is oriented parallel to of drive screw, so as to for other
Connected component provides power.
Coupling assembly is metal or plastic material system, and by sampling pond piston, positioning baffle is coupled together with slide block；
Sampling pond piston is driven together to move with it with positioning baffle when slide block is moved.
Position sensor to being made up of two slot optical couplings of full position sensor and empty position sensor, each position
Covers disposed on sensor has detect tank；Positioning baffle is made up of light-proof material, its two ends respectively have a projection and
It is in opposite direction, when slide block drives sampling pond piston together to move with it with positioning baffle, on positioning baffle
When projection can be inserted into the detect tank that position sensor is provided with, cause the signal intensity of position sensor, so as to
Indicate sampling pond piston location.
It solves microalgae and microorganism near-wall air curtain are to optical measurement during high concentration microdisk electrode
Affect, while being integrated with various optical measurement modules, reduce the volume of equipment while reduces cost, simplifies
Connection, reduces integrated difficulty, is highly suitable as microdisk electrode online detection instrument.
Description of the drawings
Fig. 1 is the online microalgae automatic sampling optical measuring system schematic diagram of this patent；
Fig. 2 is that the online microalgae automatic sampling optical measuring system dead ahead view of this patent is intended to；
Fig. 3 is this patent motor slide unit group and coupling assembly and positioning baffle connection diagram；
Fig. 4 is this patent slide block and coupling assembly and sampling pond assembling schematic diagram；
Fig. 5 covers schematic appearance after framework enclosing cover for this patent；
Fig. 6 is this patent sampling pond and measurement module group schematic diagram；
In figure：1 framework, 2 motor slide unit groups, 3 coupling assemblies, 4 position sensors pair, 5 positioning baffles, 6
Sampling pond, 7 measurement module groups, 8 measurement pipe locator cards pair, 1-1 frame bases, 1-2 framework dividing plates, 1-3
Framework enclosing cover, 1-4 connected components pass through hole, 2-1 slid platform bases, 2-2 motor, 2-3 drive screws, 2-4
Slide block, 3-1 slide block contiguous blocks, 3-2 piston rod traction grooveds, 3-3 latch segments, the full position sensors of 4-1,4-2
Empty position sensor, 6-1 sampling pond outer tube, 6-2 sampling pond pistons, 8-1 left end locator cards, 8-2 right-hand members
Locator card.
Fig. 7 is OD measurement module principles；Monochromatic LED light source and signal transducer are provided with the both sides in sampling pond
And its line is vertical with the axis in sampling pond, between monochromatic LED light source and sampling pond half-reflection and half-transmission is provided with
The 45 degree of angles that are wired to of mirror and monochromatic LED light source and signal transducer, reflection and the transmission of half-reflecting half mirror
Than for 1:9；It is provided with reference to sensing in put vertical with the line of signal transducer with monochromatic LED light source
Device, the axis same with the intersection point of the line of signal transducer with monochromatic LED light source half anti-half of reference sensor
Lens overlap with monochromatic LED light source with the intersection point of the line of signal transducer；Half-reflecting half mirror makes monochromatic LED
The light that light source is projected has 9/10 to inject signal transducer through sampling pond through rear, while there is 1/10 light
Line injects reference sensor after being reflected by half-reflecting half mirror；The ratio of the signal of signal transducer and reference sensor
Value passes through the OD values that can be calculated sample.
Fig. 8 is turbidimetry module principle；The light that monochromatic LED light source is projected is vertical with the axis in sampling pond,
Between monochromatic LED light source and sampling pond a reflection and transmission ratio is provided with for 1:9 half-reflecting half mirror, and half anti-half
Lens are with light into 45 degree of angles；Half-reflecting half mirror make monochromatic LED light source project light 1/10 along with
The vertical direction of light that monochromatic LED light source is projected is propagated, and is injected and be set in this reflection light path
On reference sensor in；Half-reflecting half mirror makes the 9/10 of the light of monochromatic LED light source injection pass through half anti-half
Lens inject sampling pond, are irradiated on the fluid sample in sampling pond and produce scattered light；In sampling pond incident illumination
Sensing is provided with direction simultaneously vertical with incident illumination and sampling pond axis on sampling pond axis intersection point
Device, for the scattered light that sample in collection sampling pond is produced；
Signal transducer passes through the turbidity value that can be calculated sample with the ratio of the signal of reference sensor.
Fig. 9 is RGB measurement module principles；Light source is provided with the both sides in sampling pond to sense with signal rgb color
Device and its line are vertical with the axis in sampling pond, be provided between light source and sampling pond half-reflecting half mirror and
Light source is wired to 45 degree of angles with signal rgb color sensor, and reflection and the transmittance of half-reflecting half mirror are
1:9；Pass with reference to rgb color being provided with light source and vertical the putting of line of signal rgb color sensor
Sensor, with reference to the friendship of the line of the axis and light source and signal rgb color sensor of rgb color sensor
Point overlaps with half-reflecting half mirror with light source with the intersection point of the line of signal rgb color sensor；Light source is to send out
The light source such as White LED of wave-length coverage covering visible light scope is penetrated, xenon lamp, Halogen light etc. is flashed；Half is anti-
Pellicle mirror makes the light that light source is projected have 9/10 to inject signal rgb color sensor through sampling pond through rear,
There is 1/10 light to inject with reference to rgb color sensor after being reflected by half-reflecting half mirror simultaneously；Signal RGB color
Color sensor passes through the rgb value that can be calculated sample with the ratio of the signal with reference to rgb color sensor.
Specific embodiment
This patent is further described with reference to the accompanying drawings and examples：
Fig. 1 gives this patent online microalgae automatic sampling optical measuring system schematic diagram, including framework 1,
Motor slide unit group 2, coupling assembly 3, to 4, positioning baffle 5 samples pond 6 to position sensor, measures mould
Block group 7, measurement pipe locator card is to 8.One of ingredient of its middle frame 1 framework enclosing cover 1-3 is not represented；
Support of the framework 1 as whole system；Motor slide unit group 2 is installed on framework 1, by coupling assembly 3
Connection positioning baffle 5 and sampling pond 6；Position sensor is positioned on framework 1 to 4；Measurement module group 7 is led to
Cross the location hole arranged in measurement module group 7 to be fixed on framework 1, while the survey at the middle part of measurement module group 7
Buret can be fixed in sampling pond 6 by hole, and 8 positioning action is most sampled at last with reference to measurement pipe locator card
Pond 6 is accurately fixed.
Fig. 2 gives this patent online microalgae automatic sampling optical measuring system dead ahead view schematic diagram, wherein
Including frame base 1-1, framework dividing plate 1-2, coupling assembly passes through hole 1-4, slide block contiguous block 3-1, living
Stopper rod traction grooved 3-2, latch segment 3-3, full position sensor 4-1, empty position sensor 4-2,5 positioning gear
Plate, sampling pond 6, measurement module group 7 (including measurement module 7-1, measurement module 7-2, measurement module 7-3),
Left end locator card 8-1, right-hand member locator card 8-2.Slide block contiguous block 3-1 passes through hole 1-4 through coupling assembly,
The part and piston rod traction grooved 3-2, latch segment 3-3 for exposing framework dividing plate 1-2 is connected by location hole；It is left
End locator card 8-1, right-hand member locator card 8-2 are fixed on framework dividing plate 1-2 by location hole；Measurement module group
7 are connected with frame base 1-1 by location hole；Sampling pond 6 is inserted into the measuring cell of measurement module group 7 and passes through
In hole, while the ear for sampling pond 6 is embedded into left end locator card 8-1, in the groove of right-hand member locator card 8-2,
So that sampling pond 6 is fixed；Full position sensor 4-1, empty position sensor 4-2 pass through self poisoning hole
And the locating slot opened up on dividing plate 1-2, it is positioned on dividing plate 1-2, by what is opened up along on dividing plate 1-2
Locating slot movement can change full position sensor 4-1, and empty position sensor 4-2 is in the position of dividing plate 1-2；
Positioning baffle 5 is arranged on slide block contiguous block 3-1 by location hole, and the two ends of positioning baffle 5 are conformed with,
And it is in opposite direction.
Fig. 3 gives this patent motor slide unit group and coupling assembly and positioning baffle connection diagram, including
Slid platform base 2-1, motor 2-2, drive screw 2-3, slide block 2-4, slide block contiguous block 3-1, piston rod
Traction grooved 3-2, latch segment 3-3, positioning baffle 5.Motor 2-2 is arranged on one end of slid platform base 2-1,
And be connected with drive screw 2-3 by shaft coupling, the other end of drive screw 2-3 is connected to cunning by bearing
Platform base 2-1 relative with motor 2-2 side, and make in drive screw 2-3 and slid platform base 2-1
Line is parallel, and motor 2-2 can drive drive screw 2-3 to rotate；Slide block 2-4 is by its inside and biography
The screw thread that dynamic screw rod 2-3 matches is connected with drive screw 2-3, when motor 2-2 drives drive screw 2-3
Slide block 2-4 can be moved along drive screw 2-3 during rotation；Slide block contiguous block 3-1 is by location hole and slide block
2-4 connects, and positioning baffle 5, piston rod traction grooved 3-2, latch segment 3-3 is connected by location hole with slide block
Block 3-1 connects, and is finally sequentially followed successively by positioning baffle 5, slide block contiguous block 3-1, piston rod traction grooved 3-2,
Latch segment 3-3.
Fig. 4 is this patent slide block and coupling assembly and sampling pond assembling schematic diagram, including slide block 2-4, is slided
Block contiguous block 3-1, piston rod traction grooved 3-2, latch segment 3-3, sample pond outer tube 6-1, sample pond piston
6-2.Slide block contiguous block 3-1 is connected by location hole with slide block 2-4；Pond outer tube 6-1 is by glass, stone for sampling
The good material of the light transmission such as English or plastics is made, and a sample tap is closed and be provided with one end, and sample tap is for always
The projection of footpath 2mm to 3mm, there is the hole inside straight-through sampling pond outer tube 6-1, the diameter 1mm in hole inside projection
To 1.5mm, the sampling pond outer tube 6-1 other ends are opened, and the inside for making sampling pond outer tube 6-1 is one end with taking
The cylinder of sample mouth；The outer radius portion of sampling pond outer tube 6-1 open ends is provided with two relative ears, can be used for solid
Surely pond outer tube 6-1 is sampled；Sampling pond piston 6-2 one end is living by made by relatively soft rubber or silica gel material
Plug, middle part is sampling pond piston brace rod, and the other end is provided with a cylindrical projections, the diameter of cylindrical projections
Identical with the width of piston rod traction grooved 3-2 inside U-type groove, thickness and the piston rod of cylindrical projections draw
The thickness of groove 3-2 is identical；Latch segment 3-3 is provided with a U-type groove, and width and the sampling pond piston of U-type groove connect
The diameter of extension bar is identical；The cylindrical projections that sampling pond piston 6-2 one end is provided with during installation are embedded into piston rod
In the U-type groove of traction grooved 3-2, sampling pond piston brace rod is embedded in the U-type groove on latch segment 3-3,
After location hole is connected with slide block contiguous block 3-1, sampling pond is lived for piston rod traction grooved 3-2, latch segment 3-3
Plug 6-2 and slide block contiguous block 3-1, piston rod traction grooved 3-2, latch segment 3-3 link together.
Fig. 5 covers schematic appearance after framework enclosing cover for this patent, including frame base 1-1, outside framework
Lid 1-3, samples pond 6.The sample tap that sampling pond 6 is provided with when this product is used upward, can be by algae solution
Bubble discharges sampling pond using the release campaign of the sampling inner carrier of pond 6.
Fig. 6 is this patent sampling pond and measurement module group schematic diagram, and the measurement module group 7 of this figure is by three modules
Composition, is respectively chlorophyll fluorescence module 7-1, turbidimetry module 7-2, absorbance (OD) measurement module
7-3, the continuous on-line measurement data of 7 days.Curve A is the straight Fv/Fm of chlorophyll fluorescence, curve B in figure
For chlorophyll fluorescence maximum Fm, curve C is basic chlorophyll fluorescence F0, and curve D is absorbance (OD),
Curve E is turbidity value.
The using method of the online microalgae automatic sampling optical measuring system of this patent is：
This patent online microalgae automatic sampling optical measuring system has three kinds of actions when using：1. pipette samples：
Motor rotation simultaneously drives sampling pond piston to move to full position sensor direction, until positioning baffle causes full
Position sensor signal changes.2. sample is emptied：Motor rotation simultaneously drives sampling pond piston to pass to empty position
Sensor direction is moved, until positioning baffle causes empty position sensor signal to change.3. measure：Complete to inhale
After taking sample action, each measurement module is sequentially completed measurement under control circuit control.The online microalgae of this patent
Automatic sampling optical measuring system has two kinds of operational modes when using：First, clean：Motor is with faster
Speed is run, and is continuously finished multiple pipette samples, the do action of emptying sample, is eventually positioned at emptying sample
The state that product are completed.2nd, on-line determination：Sample is emptied after pipette samples complete once simply to sample pond and put
Rerun a pipette samples after changing, and measures after the completion of pipette samples, finally in operation single voiding sample
Product, and rest on the state that emptying sample is completed.The calibration process of measurement module is identical with on-line determination simply
Measured sample is the correcting fluid of each measurement module, finally obtains the calibration parameter that each measurement module needs,
Make measurement module data more reliable.
Claims (8)
1. online microalgae automatic sampling optical measuring system, including framework (1), motor slide unit group (2), company
Fitting group (3), position sensor to (4), positioning baffle (5), sampling pond (6), measurement module group (7),
It is characterized in that：
Motor slide unit group (2) is fixed on framework (1), by coupling assembly (3) and sampling pond (6)
Piston be connected drive sampling pond (6) on piston movement；Positioning baffle (5) is fixed on coupling assembly (3)
On, positioning baffle (5) is made up of light-proof material, is conformed with two ends, two projections it is in opposite direction,
By follow coupling assembly (3) together motion projection can on position sensor to the groove on (4) in, draw
Position sensor is played to the signal intensity of (4) so as to indicate the position of piston；Measurement module group has on (7)
The measured hole that sampling pond (6) can be allowed to pass through, and have the location hole being located by connecting with framework (1) to fix, it is fixed
Sampling pond (6) behind position is passed through from the measured hole of measurement module group (7), control of each module in control circuit
Measure under system.
2. online microalgae automatic sampling optical measuring system according to claim 1, it is characterised in that：
The framework (1) is made up of light-proof material, and framework (1) includes frame base (1-1), framework
Dividing plate (1-2), framework enclosing cover (1-3), its middle frame dividing plate (1-2) is provided with coupling assembly through hole (1-4)；
Its middle frame dividing plate (1-2) is placed in frame base (1-1) top, framework dividing plate (1-2) and frame base
(1-1) bottom surface is parallel, and electricity is installed between the bottom surface of framework dividing plate (1-2) and frame base (1-1)
Machine slide unit group (2)；Framework enclosing cover (1-3) is fastened on frame base (1-1) top, framework enclosing cover (1-3)
With frame base (1-1) jointly around a closed cavity is formed, framework dividing plate (1-2) is located at airtight cavity
It is interior, after framework enclosing cover (1-3) and frame base (1-1) and framework dividing plate (1-2) are connected, composition one
Individual closed cavity, makes internal structure disturb from extraneous light.
3. according to the online microalgae automatic sampling optical measuring system of claim 1 or 2 institutes, it is characterised in that：
Motor slide unit group (2) is by slid platform base (2-1), and motor (2-2), drive screw (2-3) is sliding
Block (2-4) is constituted；Wherein motor (2-2) is fixed on the one end in slid platform base (2-1), motor
Power output shaft is connected by shaft coupling with drive screw (2-3), and drive screw (2-3) other end passes through
Bearing is fixed on slid platform base (2-1) other end relative with motor (2), makes drive screw (2-3)
It is parallel with the bottom surface of slid platform base (2-1)；In slide block (2-4) band is provided with drive screw (2-3) screw thread
Match the through hole of female thread, and drive screw is set in the through hole of slide block (2-4), motor (2) band
Dynamic drive screw (2-3) can make slide block (2-4) being oriented parallel to along drive screw (2-3) when rotating
Drive screw (2-3) axial reciprocating is moved.
4. online microalgae automatic sampling optical measuring system according to claim 1, it is characterised in that：
Coupling assembly (3) is by slide block contiguous block (3-1), piston rod traction grooved (3-2), latch segment (3-3)
Composition；Slide block contiguous block (3-1) is L-type, and one end is connected with slide block (2-4), and the other end is provided with fixed
Position hole, matches with the location hole on piston traction grooved (3-2) and latch segment (3-3), can lead in piston rod
Approaching channel (3-2), together with latch segment (3-3) is bolted with slide block contiguous block (3-1), so as to
Move with slide block (2-4)；Slide block contiguous block (3-1) is disposed through on framework dividing plate (1-2)
Rectangle coupling assembly is connected through hole (1-4) with slide block (2-4)；Piston rod traction grooved (3-2) and lock
A U-type groove is respectively provided with tight block (3-3), the width of the U-type groove on piston rod traction grooved (3-2) is more than
The width of U-type groove on latch segment (3-3).
5. online microalgae automatic sampling optical measuring system according to claim 1, it is characterised in that：
Sampling pond (6) samples pond by sampling pond outer tube (6-1), sampling pond piston (6-2) composition during installation
(6) axial direction is parallel with the axial direction of drive screw (2-3)；Wherein sampling pond outer tube (6-1) is
The good glass of light, quartz or plastic material are made, and in its one end injection port is provided with, and injection port is hollow
External diameter is that 2 to 3mm cylindrical protrusion is used to connect probe tube；Sampling pond piston (6-2) is made up of three parts,
One end is the piston head that an external diameter agrees with the interior Jing phases of sampling pond outer tube (6-1), and middle part is piston brace rod,
The other end is provided with a cylindrical projections, has a diameter larger than piston brace rod less than or equal to piston rod traction grooved
(3-2) width of the U-type groove being provided with, the height of cylindrical projections is equal to piston rod traction grooved (3-2)
Thickness；Open on embedded piston rod traction grooved (3-2) of cylindrical projections of sampling pond piston (6-2) one end
If U-type groove in, and after latch segment (3-3) is compressed by bolt through location hole and slide block contiguous block (3-1)
Link together, final sampling pond piston (6-2) can be moved with slide block (2-4).
6. online microalgae automatic sampling optical measuring system according to claim 1, it is characterised in that：
Position sensor is to (4) by two grooves of full position sensor (4-1) and empty position sensor (4-2)
Type optocoupler is constituted, and by the locating slot being arranged on framework dividing plate (1-2) framework dividing plate (1-2) is fixed on
Dorsad away from the side of motor slide unit group (2)；Positioning baffle (5) is made up of light-proof material, its two
Respectively there is a projection at end and two projection directions are conversely, positioning baffle (5) and slide block contiguous block (3-1)
Connected by location hole so as to cunning can be followed by the connection of slide block contiguous block (3-1) and slide block (2-4)
Block (2-4) is mobile；The projection at positioning baffle (5) two ends, one towards on full position sensor (4-1)
The detect tank of setting, detect tank of another projection towards position sensor (4-2)；When positioning baffle (5)
When moving to full position sensor (4-1) and being close to full position sensor (4-1), positioning baffle (5)
In the detect tank of the full position sensor (4-1) of projection insertion, cause the signal of full position sensor (4-1)
Change；When positioning baffle (5) is to empty position sensor (4-2) is mobile and close empty position sensor (4-2)
When, in the detect tank of projection insertion empty position sensor (4-2) of positioning baffle (5), cause empty position
The signal intensity of sensor (4-2).
7. online microalgae automatic sampling optical measuring system according to claim 1, it is characterised in that：
Measurement module group (7) is provided with sampling pond and consists of the measurement module in hole by multiple middle parts, quantity
Determined by sampling pond active length and block size, 1 to 4 modules typically can be installed；Measurement module is all
Optical measurement module, such as absorbance (OD) module, turbidity module, chlorophyll fluorescence module, RGB color
One or two or more kinds in module, absorption spectrum module etc.；It is equipped with sampling pond in the middle part of each module to lead to
Via, sampling pond is equal with the external diameter of sampling pond outer tube (6-1) by the internal diameter in hole, and sampling pond is sheathed on and takes
On the outer tube of sample pond, measurement module is set closely to be connected with sampling pond outer tube (6-1), beneficial to measurement light and by light-metering
Propagation and reduce extraneous light interference.
8. online microalgae automatic sampling optical measuring system according to claim 1, it is characterised in that：
Measurement pipe locator card is made up of left end locator card (8-1) to (8) with right-hand member locator card (8-2), left
Two location holes are respectively equipped with end locator card (8-1) and right-hand member locator card (8-2), can be with framework dividing plate
(1-2) slotted eye on is bolted；Left end locator card (8-1) is each with right-hand member locator card (8-2)
A groove is provided with, the fixation that its width is provided with sampling pond outer tube (6-1) side wall is identical with ear's width,
The ear of sampling pond outer tube (6-1) is positioned at into left end locator card (8-1) with right-hand member locator card (8-2)
After in groove, sampling pond (6) is set to be limited on the direction parallel with drive screw (2-3), so as to take
Sample pond piston (6-2) can only start shipment on the direction parallel to drive screw (2-3) with slide block (2-4)
It is dynamic.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510762836.XA CN106680203A (en) | 2015-11-10 | 2015-11-10 | Online microalgae automatic sampling optical measuring system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510762836.XA CN106680203A (en) | 2015-11-10 | 2015-11-10 | Online microalgae automatic sampling optical measuring system |
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| Application Number | Title | Priority Date | Filing Date |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113218867A (en) * | 2021-05-17 | 2021-08-06 | 燕山大学 | Fixed bed frame of red tide algae recognition device |
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| CN101308098A (en) * | 2008-06-26 | 2008-11-19 | 山东大学 | Dynamic scanning type paper money breakage rate recognition instrument and its working method |
| CN101776625A (en) * | 2009-12-25 | 2010-07-14 | 湖南三德科技发展有限公司 | Ash fusion tester |
| CN104713839A (en) * | 2013-12-13 | 2015-06-17 | 中国科学院大连化学物理研究所 | Automatic online microalgae OD determinator |
| CN203881789U (en) * | 2014-04-16 | 2014-10-15 | 江苏德林环保技术有限公司 | Precise injection pump |
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Application publication date: 20170517 |