CN106665355B - A kind of rock bamboo shoot tissue cultivating and seedling method - Google Patents
A kind of rock bamboo shoot tissue cultivating and seedling method Download PDFInfo
- Publication number
- CN106665355B CN106665355B CN201611157500.1A CN201611157500A CN106665355B CN 106665355 B CN106665355 B CN 106665355B CN 201611157500 A CN201611157500 A CN 201611157500A CN 106665355 B CN106665355 B CN 106665355B
- Authority
- CN
- China
- Prior art keywords
- culture
- seedling
- bamboo shoot
- induction
- rock bamboo
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a kind of rock bamboo shoot tissue cultivating and seedling methods, include the following steps：（1）Culture medium preparation,（2）Explant preparation,（3）Inoculated and cultured and（4）Domestication carries out rock bamboo shoot tissue cultivating and seedling with the present invention, and germination rate reaches 90% or more, and breeding coefficient reaches 3 ~ 5, has the characteristics that breeding coefficient is high, reproduction speed is fast, and breed seedling out to have plant characteristics identical with parent, better resistance.The difficult problem of rock bamboo shoot breeding is significantly solved, is conducive to rock bamboo shoot and carries out scale, is factory produced.
Description
Technical field
The present invention relates to plant tissue culture seedlings-raising technical fields, and in particular to a kind of rock bamboo shoot tissue cultivating and seedling method.
Background technology
Rock bamboo shoot are the herb of orchid bamboo shoot orchid Thunia alba (Lindl.) Rchb.f., also known as logical orchid, thrum,
China pink, rock bamboo.Perennial draft of growing nonparasitically upon another plant, high about 30~50 centimetres of fibrous root majorities, meat shape, canescence or light brown.Rock
Bamboo shoot are sweet in flavor, mild-natured, according to《Yunnan Chinese herbal medicine》、《Guizhou medicine plants catalogue》With《Simao, Yunnan Chinese herbal medicine selects》It records, with cough-relieving
Relieving asthma, promoting blood circulation the effects that, for treating pneumonia, tracheae and bronchitis, gastric and duodenal ulcer, fracture and bruise labor
Wound etc..Rock bamboo shoot have very high medical value, there is huge market prospects.Because rock bamboo shoot seed has, the tiny amount of seed is big, nothing
Endosperm needs to sprout with suitable mycorrhizal fungi under natural conditions, and natural environment destroys and artificial excessively excavation, plus
Germination Condition is harsh, causes wild resource extremely difficult in original Habitat pattern.
Currently, a kind of method about rock bamboo shoot tissue cultivating and seedling not yet, has no going out for artificial growth and artificial breeding
It is existing, scale breeding and the plantation of rock bamboo shoot are constrained to a certain extent.
Invention content
The technical problem to be solved by the present invention is to：A kind of rock bamboo shoot tissue cultivating and seedling method is provided, rock bamboo shoot tissue-cultured seedling is improved
The survival rate of cultivation, it is cost-effective, provide key technology for the production of rock bamboo shoot scale breeding.
The purpose of the present invention is what is be achieved through the following technical solutions：
A kind of rock bamboo shoot tissue cultivating and seedling method, includes the following steps：
（1）It is prepared by culture medium：Culture medium includes induction germination medium, proliferated culture medium and root media；Wherein, it lures
It is that MS+NAA0.2mg/L+KT0.5mg/L+ spends No. 1 0.7g/L+ banana puree 30g/L+ mashed potatoess 20g/L+ of treasured to lead germination medium
Sucrose 30g/L+ agar 5g/L+PH5.9；Proliferated culture medium is MS+IBA0.5mg/L+6-BA0.5mg/L+ sucrose 30g/L+ agar
5g/L+PH5.9；Root media is 2/3MS+NAA0.8mg/L+IAA0.5mg/L+ sucrose 30g/L+ activated carbon 0.5g/L+ fine jades
Fat 5g/L+ph5.9；Prepared induction germination medium is packed into culture bottle with every bottle of 60ml, every bottle of 120ml of root media
In, it is carried out using 121 DEG C, 0.5Mpa spare after sterilizing 25min postcoolings solidify；
（2）It is prepared by explant：Autumn picks full outstanding achievement as explant on ripe plant, and is cleaned, disappeared
Poison；
（3）Inoculated and cultured：Inoculation utensil sterilizes, after both hands disinfection, by step（2）In the explant that is prepared exist
In superclean bench, outstanding achievement is cut by sterile working requirement, seed therein is uniformly sprinkled into induction germination medium,
Cover and carry out induction in the standard culture room after being positioned over disinfection after bottle cap and sprout culture, accessed after emergence in root media into
Row culture of rootage 2 ~ 3 months；
（4）Domestication：Culture of rootage is obtained seedling to take out, flowing water is planted after rinsing after impregnating 10min with 1000 times of carbendazim
Plant in peat soil, perlite, wood sawdust mixed-matrix, domestication initial stage with 3 needle shading nets shade 10 days, tame 60 days it is i.e. removable
Plant crop field.
Preferably, step（2）The step of cleaning of middle explant, disinfection is：It is rinsed well after being steeped 30 minutes with soap water enchroachment (invasion)
Superficial stain, then flowing water rinse at least 30min, with pocket knife by the scar removal in outstanding achievement, after surface moisture evaporation, super
5 ~ 10min of bubble is invaded with 75% ethyl alcohol on net workbench, constantly rocks outstanding achievement halfway, then with using nothing after aseptic water washing 3 ~ 5 times
The taking-up of bacterium tweezers, which is placed in a concentration of 0.1 ~ 0.2% mercuric chloride, invades 10 ~ 15min of bubble, and outstanding achievement is constantly rocked in midway, then uses sterile water
It is placed on the inoculation disk for being lined with sterile blotting paper with aseptic nipper taking-up after rinsing 5 ~ 8 times, you can.
Preferably, step（3）In, induction sprouting culture is carried out in culturing room, the concrete operations of culture of rootage are：It is training
Foster interior carries out induction to sprout culture including light culture and optical culture, and temperature is controlled in 18 ~ 22 DEG C, humidity 40- when light culture
50%, light culture is transferred to optical culture, daily 8 hours of optical culture illumination 3500LUX after 7 ~ 10 days；Wait for that culture height of seedling 2 is sprouted in induction
~ 3cm, seedling of the stalk diameter more than 0.3cm, which is transferred in superclean bench by sterility requirements, takes root in root media
Culture, culture of rootage temperature are 25 DEG C, daily 8 hours of illumination 3500LUX；Other seedling can go in proliferated culture medium expand it is numerous
It grows.
Preferably, step（4）In, peat soil, perlite, wood sawdust three volume ratio be 4：1：1, it is mixed to obtain
Planting matrix PH6.5 ~ 6.8.
Beneficial effects of the present invention：Rock bamboo shoot tissue cultivating and seedling is carried out with the present invention, germination rate reaches 90% or more, numerous
It grows coefficient and reaches 3 ~ 5, have the characteristics that breeding coefficient is high, reproduction speed is fast, and breed seedling out to have and parent phase
Same plant characteristics, better resistance.The difficult problem of rock bamboo shoot breeding is significantly solved, is conducive to rock bamboo shoot and carries out scale, batch production
Production.
Specific implementation mode
Invention is described further with reference to specific embodiment：
A kind of rock bamboo shoot tissue cultivating and seedling method, includes the following steps：
（1）It is prepared by culture medium：Culture medium includes induction germination medium, proliferated culture medium and root media；Wherein, it lures
It is that MS+NAA0.2mg/L+KT0.5mg/L+ spends No. 1 0.7g/L+ banana puree 30g/L+ mashed potatoess 20g/L+ of treasured to lead germination medium
Sucrose 30g/L+ agar 5g/L+PH5.9；Proliferated culture medium is MS+IBA0.5mg/L+6-BA0.5mg/L+ sucrose 30g/L+ agar
5g/L+PH5.9；Root media is 2/3MS+NAA0.8mg/L+IAA0.5mg/L+ sucrose 30g/L+ activated carbon 0.5g/L+ fine jades
Fat 5g/L+ph5.9；Prepared induction germination medium is packed into culture bottle with every bottle of 60ml, every bottle of 120ml of root media
In, it is carried out using 121 DEG C, 0.5Mpa spare after sterilizing 25min postcoolings solidify.
（2）It is prepared by explant：Autumn picks full outstanding achievement as explant on ripe plant, and is cleaned, disappeared
Poison, step are：Superficial stain is rinsed well after being steeped 30 minutes with soap water enchroachment (invasion), and then flowing water rinses at least 30min, will with pocket knife
Scar removal in outstanding achievement invades 5 ~ 10min of bubble, midway is not on superclean bench after surface moisture evaporation with 75% ethyl alcohol
It is disconnected to rock outstanding achievement, it then uses to take out to be placed in a concentration of 0.1 ~ 0.2% mercuric chloride with aseptic nipper after aseptic water washing 3 ~ 5 times and invade
Steep 10 ~ 15min, outstanding achievement is constantly rocked in midway, then with taken out with aseptic nipper after aseptic water washing 5 ~ 8 times be placed on be lined with it is sterile
On the inoculation disk of blotting paper, you can.
（3）Inoculated and cultured：Inoculation utensil sterilizes, after both hands disinfection, by step（2）In the explant that is prepared exist
In superclean bench, outstanding achievement is cut by sterile working requirement, seed therein is uniformly sprinkled into induction germination medium,
It covers and carries out induction in the standard culture room after being positioned over disinfection after bottle cap and sprout culture, culture of rootage, concrete operations are as follows：
Induction sprouting culture is carried out in culturing room, the concrete operations of culture of rootage are：Induction sprouting culture is carried out in culturing room includes
Light culture and optical culture, in 18 ~ 22 DEG C, humidity 40-50%, light culture is transferred to light training after 7 ~ 10 days for temperature control when light culture
It supports, daily 8 hours of optical culture illumination 3500LUX；Wait for that culture 2 ~ 3cm of height of seedling is sprouted in induction, seedling of the stalk diameter more than 0.3cm exists
It transfers by sterility requirements in superclean bench and carries out culture of rootage in root media, culture of rootage temperature is 25 DEG C, illumination
Daily 8 hours of 3500LUX；Other seedling can go to expanding propagation in proliferated culture medium.
（4）Domestication：Culture of rootage is obtained seedling to take out, flowing water is planted after rinsing after impregnating 10min with 1000 times of carbendazim
Plant in peat soil, perlite, wood sawdust mixed-matrix, peat soil, perlite, wood sawdust three volume ratio be 4：1：1, institute
Planting matrix PH6.5 ~ 6.8 being mixed to get, domestication initial stage are shaded 10 days with 3 needle shading nets, and domestication can transplant crop field in 60 days.
The above content is a further detailed description of the present invention in conjunction with specific preferred embodiments, and it cannot be said that
The specific implementation of the present invention is confined to these explanations.For those of ordinary skill in the art to which the present invention belongs, exist
Under the premise of not departing from present inventive concept, a number of simple deductions or replacements can also be made, all shall be regarded as belonging to the present invention's
Protection domain.
Claims (3)
1. a kind of rock bamboo shoot tissue cultivating and seedling method, which is characterized in that include the following steps：
（1）It is prepared by culture medium：Culture medium includes induction germination medium, proliferated culture medium and root media；Wherein, induction is sprouted
Hair culture medium is that MS+NAA0.2mg/L+KT0.5mg/L+ spends precious No. 1 0.7g/L+ banana puree 30g/L+ mashed potatoes 20g/L+ sucrose
30g/L+ agar 5g/L+pH5 .9；Proliferated culture medium is MS+IBA0.5mg/L+6-BA0.5mg/L+ sucrose 30g/L+ agar 5g/
L+pH5 .9；Root media is 2/3MS+NAA0.8mg/L+IAA0.5mg/L+ sucrose 30g/L+ activated carbon 0.5g/L+ agar
5g/L+ph5.9；Prepared induction germination medium is packed into culture bottle with every bottle of 60ml, every bottle of 120ml of root media
In, it is carried out using 121 DEG C, 0.5Mpa spare after sterilizing 25min postcoolings solidify；
（2）It is prepared by explant：Autumn picks full outstanding achievement as explant on ripe plant, and is cleaned, sterilized；
（3）Inoculated and cultured：Inoculation utensil sterilizes, after both hands disinfection, by step（2）In the explant that is prepared ultra-clean
In workbench, outstanding achievement is cut by sterile working requirement, seed therein is uniformly sprinkled into induction germination medium, is covered
It is positioned over after bottle cap in the standard culture room after disinfection and carries out induction sprouting culture, accessed in root media and given birth to after emergence
Root culture 2 ~ 3 months；
（4）Domestication：Culture of rootage is obtained seedling to take out, with 1000 times of carbendazim impregnate after flowing water after 10min rinses plantation in
In peat soil, perlite, wood sawdust mixed-matrix, domestication initial stage is shaded 10 days with 3 needle shading nets, and domestication can transplant big for 60 days
Field, wherein peat soil, perlite, wood sawdust three volume ratio be 4：1：1, mixed obtained planting matrix pH6 .5 ~
6.8。
2. a kind of rock bamboo shoot tissue cultivating and seedling method according to claim 1, it is characterised in that：Step（2）Middle explant
Cleaning, disinfection the step of be：Superficial stain is rinsed well after being steeped 30 minutes with soap water enchroachment (invasion), then flowing water rinses at least 30min,
With pocket knife by the scar removal in outstanding achievement, after surface moisture evaporation, on superclean bench with 75% ethyl alcohol immersion 5 ~
Outstanding achievement is constantly rocked in 10min, midway, then use taken out with aseptic nipper after aseptic water washing 3 ~ 5 times be placed on a concentration of 0.1 ~
10 ~ 15min is impregnated in 0.2% mercuric chloride, outstanding achievement is constantly rocked in midway, then is taken out with aseptic nipper with after aseptic water washing 5 ~ 8 times
It is placed on the inoculation disk for being lined with sterile blotting paper, you can.
3. a kind of rock bamboo shoot tissue cultivating and seedling method according to claim 1, it is characterised in that：Step（3）In, it is cultivating
Induction sprouting culture is carried out in room, the concrete operations of culture of rootage are：It carries out induction in culturing room to sprout culture including dark training
Support and optical culture, when light culture temperature control be transferred to optical culture, light after 7 ~ 10 days in 18 ~ 22 DEG C, humidity 40-50%, light culture
Cultivate illumination 3500LUX daily 8 hours；Wait for that culture 2 ~ 3cm of height of seedling is sprouted in induction, seedling of the stalk diameter more than 0.3cm is ultra-clean
It transfers by sterility requirements in workbench and carries out culture of rootage in root media, culture of rootage temperature is 25 DEG C, illumination
Daily 8 hours of 3500LUX；Other seedling can go to expanding propagation in proliferated culture medium.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611157500.1A CN106665355B (en) | 2016-12-15 | 2016-12-15 | A kind of rock bamboo shoot tissue cultivating and seedling method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611157500.1A CN106665355B (en) | 2016-12-15 | 2016-12-15 | A kind of rock bamboo shoot tissue cultivating and seedling method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106665355A CN106665355A (en) | 2017-05-17 |
| CN106665355B true CN106665355B (en) | 2018-10-02 |
Family
ID=58869288
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611157500.1A Active CN106665355B (en) | 2016-12-15 | 2016-12-15 | A kind of rock bamboo shoot tissue cultivating and seedling method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106665355B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107466858B (en) * | 2017-09-25 | 2019-12-03 | 贵州大学 | A kind of bamboo shoot orchid species seedling rapid propagation method |
| CN115644059B (en) * | 2022-10-08 | 2023-07-11 | 贵州省植物园（贵州省园林科学研究所、贵州省植物研究所） | Tissue culture and rapid propagation method for borage |
-
2016
- 2016-12-15 CN CN201611157500.1A patent/CN106665355B/en active Active
Non-Patent Citations (1)
| Title |
|---|
| 笋兰的组织培养初报;梁国平;《广西热作科技》;19991231(第2期);第39页第1节至第4.3节 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106665355A (en) | 2017-05-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104067821B (en) | A kind of preparation method of Sweetpotato Viruses Elimination seedling | |
| CN108307823B (en) | Method for performing cutting propagation by using blueberry tissue culture seedlings | |
| CN101116424B (en) | Highly effective lily bulblet inducement culture method | |
| CN107047310A (en) | A kind of cultural method of bletilla striata seeds culture seedling | |
| CN101595824B (en) | Rapid in-vitro seedling raising method by utilizing sandalwood seed embryo | |
| CN106922534A (en) | A kind of method that tissue cultures quickly breed China fir | |
| CN104585036B (en) | The tissue culture and rapid propagation method of the fiery axillalry bud on Malus spectabilis plateau, a kind of North America | |
| CN106359087A (en) | Tissue culture quick-breeding seedling raising method for radix asparagi | |
| CN107197746A (en) | A kind of mating system of China fir field excellent resources | |
| CN108575759B (en) | A method of bletilla striata tissue-culturing rapid propagation is carried out using carbon nanotube | |
| CN106665355B (en) | A kind of rock bamboo shoot tissue cultivating and seedling method | |
| CN110301337A (en) | A kind of rubber tree cuttage and seedling culture method | |
| CN108782247A (en) | A kind of method for tissue culture of late cherry " Yu Yihuang " kind of Japan | |
| CN105409748B (en) | A kind of fast breeding method of extra large scirpus scirpus | |
| CN108651275A (en) | A kind of method of quickly breeding bletilla striata seedling under natural light | |
| CN105557515A (en) | Tissue culture fast propagation method of neottopteris nidus avis | |
| CN113598046B (en) | Efficient tissue culture breeding method for ficus microcarpa | |
| CN105684910B (en) | The tissue culture and rapid propagation method of dendrobium hancockii Rolfe | |
| CN105918124B (en) | A kind of method of chrysanthemum Herba Tricyrtidis macropodae tissue culture and rapid proliferation | |
| CN101695282A (en) | Method for improving transplant survival rate of test tube dendrobium devonianum seedlings by resistance processing | |
| CN108812310A (en) | A kind of efficient method for inducing magnificent Paris polyphylla sapling multiplication | |
| CN108450335A (en) | A kind of fresh water sand pear stem section tissue rapid propagation method | |
| CN113875585A (en) | Method for in-vitro rapid propagation and seedling raising of roxburgh rose | |
| CN105794650A (en) | Method for preserving minimum population Guangxi bilberry offspring by means of immature seeds | |
| CN110150146A (en) | A kind of blood aspidistra tissue culture propagation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20201228 Address after: No. 705, No. 2 Building, Guantang R&D Center, No. 10 Shuangren Road, Liudong New District, Liuzhou City, Guangxi Zhuang Autonomous Region (Trusted by Liuzhou Gaochuang Business Secretary Co., Ltd.) Patentee after: Liuzhou Shuangcheng Technology Co.,Ltd. Address before: No.7 building, shuangshuixiang Hejia, Shuicheng County, Liupanshui City, Guizhou Province Patentee before: LIUPANSHUI QIANFENG AGRICULTURAL DEVELOPMENT Co.,Ltd. |
|
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20210317 Address after: 211111 No. 12, Mazhou East Road, Mau Ling Street, Jiangning District, Nanjing, Jiangsu Patentee after: JIANGSU ZHIJU INTELLECTUAL PROPERTY SERVICE Co.,Ltd. Address before: No. 705, No. 2 Building, Guantang R&D Center, No. 10 Shuangren Road, Liudong New District, Liuzhou City, Guangxi Zhuang Autonomous Region (Trusted by Liuzhou Gaochuang Business Secretary Co., Ltd.) Patentee before: Liuzhou Shuangcheng Technology Co.,Ltd. |
|
| TR01 | Transfer of patent right |