CN106649206B - Seed genoid engineered crop is mixed into Risk Monitoring sampling method for making sample - Google Patents
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Abstract
Seed genoid engineered crop is mixed into Risk Monitoring sampling method for making sample, including the following steps: 1. construct the unit number of primary sample using standardized normal distribution；2. reserved fiducial range width simultaneously reconfigures sampling unit；3. being defined again using sampler unitary sampling amount as unit overall and calculating required Unit numbers；4. the sampler frequency in sampling needed for being calculated with the Unit numbers of step 2.；5. with the confidence level and deviation of total sample Unit numbers obtained by t Evaluation on distribution single sample；6. carrying out double sampling using division method, and evaluated with t distribution；7. carrying out crushing sample preparation operation.The present invention is suitable for the genetic engineering sampling Detections (be particularly suitable for edible seed and reserve seed for planting with seed) of all kinds of seed class crops, the risk control for being mixed into ratio suitable for different genes engineering crop, the sampling occasion suitable for different confidence levels and deviation requirement, the testing cost management for being mixed into suitable for gene engineering crop seed risk control.
Description
Technical field
The present invention relates to a kind of detection of genetic engineering crops sampling method for making sample.
Background technique
Application of the genetic engineering in crops is very universal.Currently, the soybean import of China has reached
More than 8000 ten thousand tons, and also increasing, other gene engineering crops, such as wheat, rice, rapeseed are also extensive all over the world
Carry out plantation research, or even has been commercialized.Gene engineering crop seed, the commercialization of grain oil product are also thriving.It is civil
Gene engineering crop and products thereof, such as rice and flour oil just enters the dining table of common people as the staple food grain of countries in the world.
The safety of gene engineering crop, common people select the preference of gene engineering crop in other words, always exist and strive
View, no matter and its dispute necessity, after all dispute be existing.
Whether crop seeds or its harvest, such as wheat, rice, rapeseed, soybean belong to gene engineering product, the world
Various countries especially all develop many detections, mirror method for distinguishing with the big economy such as the U.S., China, European Union.These methods, cover
Three aspects of sampling, sample preparation, detection of genetic engineering detection are covered.But the work of this three aspect is not to advance side by side,
That is guaranteed extent of the work of this three aspect in confidence level and the deviation control of final data result is different.Especially
It is in sampling and sample preparation link, although the two steps contribute to the major part of testing result variance, there is presently no to pumping
The introduced control errors of sample, sample preparation link have the description of quantization.
Countries in the world are concentrated mainly on the sample selection to seed crop to the sampling standard of genetic engineering crops seed material
Aspect, also, the confidence level guarantee of its seed sampling is set；Its sample unit is using seed as sampling unit；Institute
The testing result deviation control aspect for obtaining sample does not also provide explicitly.
For the demand for adapting to detection, it is also necessary to the sample that is extracted carry out division, sample preparation, but division, sample making course
Confidence level and deviation control regulation are seldom.
Each laboratory can face such problems: even if having extracted sample by standard, since the amount of single sample has
Tens even several hundred kilograms, and the amount of laboratory test sample then only has several grams of even several milligrams of the orders of magnitude, in single sample
Sample brought by the case that deviation is also not known, then several grams or several milligrams are chosen from the resulting sample of single sample
Whether obtained test result can represent totality, and confidence level and deviation will be how many.
Currently, there is also many moulds for the confidence level to the risk monitoring and control result of genetic engineering crops seed material and deviation management
Area is pasted, and its main cause, the error and confidence level introduced just because of pumping sample preparation link cannot obtain continuous control.
Summary of the invention
In view of the upper of the confidence level of the risk monitoring and control result of genetic engineering crops seed material in the prior art and deviation management
Deficiency is stated, the present invention provides a kind of seed genoid engineering agriculture that genetic engineering agricultural product risk control ability can be improved
Crop is mixed into Risk Monitoring sampling method for making sample.
The present invention the technical solution to solve the technical problem is that: seed genoid engineered crop be mixed into Risk Monitoring pumping
Sample method for making sample, including the following steps:
1. the genetic engineering seed percentage upper limit, confidence level and the relative error upper limit that is arranged using both sides of supply and demand is items
Part calculates Unit numbers, and sampling unit herein is as unit of grain；According to the weight and list of Unit numbers, single seed
The diameter calculation division constant k of seed；
2. sampling unit reconfigures:
Reserved 50% fiducial range width below, and construction sampling unit is recalculated, sampling unit herein is by several
Grain seed composition；
The sampling unit constructed with this step redefines the totality of sample for a basic unit, and 1. by step
Set confidence level and the relative error upper limit is foundation, Unit numbers needed for calculating；
3., using the unitary sampling amount of sampler as basic unit, definition is overall again, and by step 1. set confidence
Degree and the relative error upper limit are according to Unit numbers needed for calculating；
4. calculates the frequency in sampling of required sampler with the Unit numbers of step 2., as single sample number；
4. 5. calculates the confidence level and deviation of the Unit numbers of resulting single sample with t Evaluation on distribution through step, if
Single sample unit number is calculated through t distribution meets confidence range and deviation requirement, then can adjust the frequency in sampling to reduce and take out
Sample workload；If not satisfied, should then adjust sampler or sampling number, it is allowed to meet confidence range and deviation requirement；
Meet or ungratified benchmark is step 2. reserved confidence range width；
6. 5. determines that sample size is totally, to be newly defined as by 2. sampling unit that step is constructed secondary with step
Sampled population carries out double sampling, and the method for double sampling is division, obtains two according to the number of division and each division ratio
Secondary amount of sampling, and with t distribution and rated condition under confidence level and deviation recalculate the introduced confidence range of division
And deviation range, otherwise tune is passed through according to the control requirement whether calculated result evaluation double sampling meets confidence level and deviation
Whole division ratio and division number are to adjust double sampling amount；
Meet or ungratified benchmark is step 2. reserved confidence range width；
7. is with step, 6. resulting sampling total amount is overall, progress sample preparation operation, and sample preparation, which operates, to need to crush, and requires
The fineness of crushing is controlled, mainly using standard screen screening deciding grade and level, according to 1. division constant k that step is calculated come
Calculate the sampling amount of different grinding particle size samples.
Further include step 8., take out sample preparation scheme check: again calculate 4. step is sampled, 6. step is sampled, step 7. sample preparation
Synthesis confidence level (i.e. the products of three confidence levels) and relative deviation it is horizontal, according to the assessment of the error of institute's choosing method in laboratory
Decide whether to receive such sampling plan, if cannot receive can again 4. set-up procedure is sampled, 6. step is sampled, step 7.
The unit number of sample preparation.
The beneficial effects of the present invention are: suitable for the risk control for being mixed into ratio of different genes engineering crop, in quotient
In trade link, it is horizontal that different commerce and trade main bodys can propose different risk controls, for example " non-(turn) genetic engineering " arranged produces
Product are that 1%, 0.5%, 0.1% even 0.01% testing result is limited to detection to evaluate it and can receive horizontal, some detections
Limit or risk limit value are not yet covered by existing standards system, and practical operation difficulty is caused to increase, and this patent can flexibly be set
Risk control is horizontal and carries out taking out sample preparation conceptual design.
Suitable for the sampling occasion of different confidence levels and deviation requirement, for being mixed into the more stable crop kernel of ratio,
It is mixed into risk control level and can make adjustment, to reduce sampling, sample preparation workload, if desired by 99% confidence level, or
Be that 5% relative deviation is made adjustment, for example, be reduced to 90% confidence level either 10% relative deviation it is horizontal, this patent can
It is certainly, tight to add to adapt to the demand at scene rapidly according to the redesign for needing to make sampling, sample preparation scheme at scene
Risk control is horizontal, can also flexibly make adjustment.
The testing cost management of risk control is mixed into suitable for gene engineering crop seed, since this patent can assess pumping
Sample, the confidence level of sample preparation and relative deviation are horizontal, so, final detection, test specimen and initial tens tons, 1,000,110,000 tons of agricultures
Product has good unbiasedness, it is possible to reduce and single test, the Duplicate Samples number of detection can also reduce repetition detection number,
And it can be used as the generally acknowledged sampling of commerce and trade dispute process, method for making sample.
The application of this patent will greatly improve China in genetic engineering agricultural product risk control ability, promote China in base
Because of the level in engineering detecting field, China is made to have a say in this field.
Risk Monitoring described in this patent, which takes out key parameter involved in method for making sample, to be had: confidence level, is reserved relative deviation
Fiducial range width, double sampling number, division ratio, division number, smashing fineness, division constant, samples single sample number three times
Number etc., above-mentioned parameter be also the equipment such as sampler, sampling routine, reduction device, pulverizer and sampling, the research and development of sample-preparing system,
Production and manufacture provide technical conditions.
Specific embodiment
Invention is further described in detail With reference to embodiment.
Seed genoid engineered crop is mixed into Risk Monitoring sampling method for making sample, including the following steps:
1. the genetic engineering seed percentage upper limit that is arranged with both sides of supply and demand, both sides of supply and demand agreement confidence level and
The relative error upper limit of both sides of supply and demand agreement is condition, calculates Unit numbers, and sampling unit herein is with grain
Unit.The calculation method of Unit numbers is the existing calculation method in existing theory of sample survey method herein, for example, by using
China Statistics Press's in March, 1998 publishes, " theory of sample survey and the method " that Feng Shiyong, Ni Jiaxun, Zou Guohua write, the
Method in three chapters, 3.4 section is calculated.
According to the diameter calculation division constant K of Unit numbers, the weight of single seed and single seed.Calculating herein
It is carried out according to Qie Qiaote formula, Qie Qiaote formula is Q=Kd2, i.e. sampling amount=division constant × diameter square, the unit of diameter
It is mm, the unit of sampling amount Q is kg.
2. sampling unit reconfigures:
Reserved 50% fiducial range width below, and construction sampling unit is recalculated, sampling unit herein is by several
Grain seed composition.The method of calculating is that 100/(100%- reserves fiducial range width), such as reserved fiducial range width is 15%,
Then sampling unit includes 100/(100%-15% in this step) 117 seeds of ≈.
The sampling unit constructed with this step redefines the totality of sample for a basic unit, and 1. by step
Set confidence level and the relative error upper limit is foundation, Unit numbers needed for calculating.The calculating side of Unit numbers herein
Method and step 1. in calculation method as, be the existing calculation method in existing theory of sample survey method, for example, by using in
Statistic Press, state in March, 1998 publishes, " theory of sample survey and the method " that Feng Shiyong, Ni Jiaxun, Zou Guohua write, third
Method in 3.4 section of chapter is calculated.
3., using the unitary sampling amount of sampler as basic unit, definition is overall again, and by step 1. set confidence
Degree and the relative error upper limit are according to Unit numbers needed for calculating.The calculation method of Unit numbers is that existing sampling is adjusted herein
The existing calculation method in theoretical method is looked into, is published for example, by using China Statistics Press's in March, 1998, Feng Shiyong, Ni Jiaxun,
" theory of sample survey and the method " that Zou Guohua writes, the method in 3.4 section of chapter 3 are calculated.
4. calculates the frequency in sampling of required sampler with the Unit numbers of step 2., as single sample number.This
The calculation method of place's Unit numbers is the existing calculation method in existing theory of sample survey method, for example, by using China statistical
Publishing house's in March, 1998 publishes, " theory of sample survey and the method " that Feng Shiyong, Ni Jiaxun, Zou Guohua write, chapter 3 3.4
Method in section is calculated.
4. 5. calculates the confidence level and deviation of the Unit numbers of resulting single sample with t Evaluation on distribution through step, if
Single sample unit number is calculated through t distribution meets confidence range and deviation requirement, then can adjust the frequency in sampling to reduce and take out
Sample workload；If not satisfied, should then adjust sampler or sampling number, it is allowed to meet confidence range and deviation requirement.
Meet or ungratified benchmark is step 2. reserve confidence range width, is calculated if being distributed through t
Confidence range be less than or equal to the confidence range width 2. reserved of step, then be considered as and meet the requirements；If being distributed through t
The confidence range being calculated is greater than 2. confidence range width that step is reserved, then is considered as and is unsatisfactory for requiring.
6. 5. determines that sample size is totally, to be newly defined as by 2. sampling unit that step is constructed secondary with step
Sampled population carries out double sampling, and the method for double sampling is division, obtains two according to the number of division and each division ratio
Secondary amount of sampling, and with t distribution and rated condition under confidence level and deviation recalculate the introduced confidence range of division
And deviation range, otherwise tune is passed through according to the control requirement whether calculated result evaluation double sampling meets confidence level and deviation
Whole division ratio and division number are to adjust double sampling amount.
Meet or ungratified benchmark is step 2. reserve confidence range width, is calculated if being distributed through t
Confidence range be less than or equal to the confidence range width 2. reserved of step, then be considered as and meet the requirements；If being distributed through t
The confidence range being calculated is greater than 2. confidence range width that step is reserved, then is considered as and is unsatisfactory for requiring.
7. is with step, 6. resulting sampling total amount is overall, progress sample preparation operation, and sample preparation, which operates, to need to crush, and requires
The fineness of crushing is controlled, mainly using standard screen screening deciding grade and level, according to 1. division constant k that step is calculated come
Calculate the sampling amount of different grinding particle size samples.
8. takes out sample preparation scheme and checks:
4. step is sampled, 6. step is sampled, 7. the synthesis confidence level of sample preparation and relative deviation are horizontal for step for calculating again, root
The assessment for factually testing the error of indoor institute's choosing method decides whether to receive such sampling plan, if cannot receive to adjust again
Synchronizing suddenly 4. sampling, step 6. sample, the unit number of step 7. sample preparation.
The present invention is suitable for the risk control for being mixed into ratio of different genes engineering crop, different in commerce and trade link
It is horizontal that commerce and trade main body can propose different risk controls, for example " non-(turn) genetic engineering " product arranged is to detect and be limited to
1%, 0.5%, 0.1% even 0.01% testing result can receive horizontal to evaluate it, some detection limits or risk limit value are still
It is not covered by existing standards system, practical operation difficulty is caused to increase, this patent can flexibly set risk control level simultaneously
It carries out taking out sample preparation conceptual design.
Suitable for the sampling occasion of different confidence levels and deviation requirement, for being mixed into the more stable crop kernel of ratio,
It is mixed into risk control level and can make adjustment, to reduce sampling, sample preparation workload, if desired by 99% confidence level, or
Be that 5% relative deviation is made adjustment, for example, be reduced to 90% confidence level either 10% relative deviation it is horizontal, this patent can
It is certainly, tight to add to adapt to the demand at scene rapidly according to the redesign for needing to make sampling, sample preparation scheme at scene
Risk control is horizontal, can also flexibly make adjustment.
The testing cost management of risk control is mixed into suitable for gene engineering crop seed, since this patent can assess pumping
Sample, the confidence level of sample preparation and relative deviation are horizontal, so, final detection, test specimen and initial tens tons, 1,000,110,000 tons of agricultures
Product has good unbiasedness, it is possible to reduce and single test, the Duplicate Samples number of detection can also reduce repetition detection number,
And it can be used as the generally acknowledged sampling of commerce and trade dispute process, method for making sample.
The application of this patent will greatly improve China in genetic engineering agricultural product risk control ability, promote China in base
Because of the level in engineering detecting field, China is made to have a say in this field.
Risk Monitoring described in this patent, which takes out key parameter involved in method for making sample, to be had: confidence level, is reserved relative deviation
Fiducial range width, double sampling number, division ratio, division number, smashing fineness, division constant, samples single sample number three times
Number etc., above-mentioned parameter be also the equipment such as sampler, sampling routine, reduction device, pulverizer and sampling, the research and development of sample-preparing system,
Production and manufacture provide technical conditions.
Claims (2)
1. seed genoid engineered crop is mixed into Risk Monitoring sampling method for making sample, it is characterised in that include the following steps:
1. the genetic engineering seed percentage upper limit, confidence level and the relative error upper limit that is arranged using both sides of supply and demand as condition, calculate
Unit numbers, sampling unit herein is as unit of grain；According to Unit numbers, the weight of single seed and single seed
Diameter calculation division constant k；
2. sampling unit reconfigures:
Reserved 50% fiducial range width below, and construction sampling unit is recalculated, if sampling unit herein is by dry granular seed
Grain composition；
The sampling unit constructed with this step redefines the totality of sample for a basic unit, and 1. set by step
Fixed confidence level and the relative error upper limit is foundation, Unit numbers needed for calculating；
3., using the unitary sampling amount of sampler as basic unit, definition is overall again, and by step 1. set confidence level and
The relative error upper limit is according to Unit numbers needed for calculating；
4. calculates the frequency in sampling of required sampler with the Unit numbers of step 2., as single sample number；
5. 4. calculates the confidence level and deviation of the Unit numbers of resulting single sample with t Evaluation on distribution through step, if once
Unit numbers are calculated through t distribution meets confidence range and deviation requirement, then can adjust the frequency in sampling to reduce sampling work
It measures；If not satisfied, should then adjust sampler or sampling number, it is allowed to meet confidence range and deviation requirement；
Meet or ungratified benchmark is step 2. reserved confidence range width；
6. 5. determines that sample size is totally, to be newly defined as double sampling by 2. sampling unit that step is constructed with step
It is overall, double sampling is carried out, the method for double sampling is division, obtains secondary pumping according to the number of division and each division ratio
Sample amount, and with t distribution and rated condition under confidence level and deviation recalculate the introduced confidence range of division and partially
Poor range evaluates the control requirement whether double sampling meets confidence level and deviation according to calculated result, otherwise, by adjusting contracting
Divide ratio and division number to adjust double sampling amount；
Meet or ungratified benchmark is step 2. reserved confidence range width；
7. is with step, 6. resulting sampling total amount is overall, progress sample preparation operation, and sample preparation, which operates, to need to crush, and requires to powder
Broken fineness is controlled, and mainly using standard screen screening deciding grade and level, the division constant k that is 1. calculated according to step is calculated
The sampling amount of different grinding particle size samples.
2. seed genoid engineered crop as described in claim 1 is mixed into Risk Monitoring sampling method for making sample, feature exists
In: further include step 8., take out sample preparation scheme check:
4. step is sampled, 6. step is sampled, 7. the synthesis confidence level of sample preparation and relative deviation are horizontal for step for calculating again, according to reality
The assessment for testing the error of indoor institute's choosing method decides whether to receive such sampling plan, if cannot receive that step can be adjusted again
Suddenly 4. sampling, step 6. sample, the unit number of step 7. sample preparation.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1470248A2 (en) * | 2002-01-31 | 2004-10-27 | The Procter & Gamble Company | Methods for reducing contamination by genetically modified plant products |
| CN104428425A (en) * | 2012-05-04 | 2015-03-18 | 考利达基因组股份有限公司 | Methods for determining absolute genome-wide copy number variations of complex tumors |
| CN105844115A (en) * | 2016-04-01 | 2016-08-10 | 曹际娟 | Sampling model for hybrid-strain genetically modified agricultural products and establishing method of sampling model |
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- 2016-12-17 CN CN201611172075.3A patent/CN106649206B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1470248A2 (en) * | 2002-01-31 | 2004-10-27 | The Procter & Gamble Company | Methods for reducing contamination by genetically modified plant products |
| CN104428425A (en) * | 2012-05-04 | 2015-03-18 | 考利达基因组股份有限公司 | Methods for determining absolute genome-wide copy number variations of complex tumors |
| CN105844115A (en) * | 2016-04-01 | 2016-08-10 | 曹际娟 | Sampling model for hybrid-strain genetically modified agricultural products and establishing method of sampling model |
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