CN106645459B - A kind of construction method of wind-solar resources HPLC characteristic spectrums - Google Patents
A kind of construction method of wind-solar resources HPLC characteristic spectrums Download PDFInfo
- Publication number
- CN106645459B CN106645459B CN201610947350.8A CN201610947350A CN106645459B CN 106645459 B CN106645459 B CN 106645459B CN 201610947350 A CN201610947350 A CN 201610947350A CN 106645459 B CN106645459 B CN 106645459B
- Authority
- CN
- China
- Prior art keywords
- wind
- peak
- solar resources
- peaks
- phase
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
A kind of construction method of wind-solar resources HPLC characteristic spectrums, includes the following steps：（1）The preparation of reference solution：Take Puerarin reference substance appropriate, it is accurately weighed,（2）The preparation of test solution：It takes wind-solar resources finely ground, takes about 2 ~ 5g, it is accurately weighed,（3）Chromatographic condition：Using ZORBAX SB-C18 chromatographic columns, it is 3 ~ 70 that mobile phase, which is with -0.1% phosphoric acid solution of acetonitrile,：97 ~ 30 mixed solution；0.5~1.5ml/min of volume flow；DAD detectors, wavelength are 190~250nm；25~45 DEG C of column temperature, number of theoretical plate are not less than 10000,（4）It measures：Accurate respectively to draw reference solution and each 5~20 μ l of test solution, injection liquid chromatograph obtains the HPLC characteristic spectrums of wind-solar resources according to high effective liquid chromatography for measuring；It is an advantage of the invention that the quality of wind-solar resources between different batches can be monitored effectively, method has the characteristics that precision height, favorable reproducibility, is conducive to the quality of overall monitor product.
Description
Technical field
The invention belongs to field of medicaments, and in particular to the construction method of Chinese patent drug wind-solar resources HPLC characteristic spectrums.
Background technology
Wind-solar resources are by Chinese ephedra, pueraria lobata, perilla leaf, windproof, cassia twig, the root of Dahurain angelica, dried orange peel, semen armeniacae amarae, campanulaceae, sweet
Chinese herbal medicine forms grass, rhizoma zingiberis etc. ten simply.Major function is inducing diaphoresis sweating, expelling wind and cold pathogens.For body heat of catching a cold, have a headache, cough,
Nasal obstruction, the diseases such as runny nose.Existing implementing quality standards record in Chinese patent drug ministry standard, standard No. WS3-B-0045-89, standard
The method of quality control such as uncharged indentification by TLC and assay.Due to carrying out matter not to the intermediate of product, at mouth
Measure the effective means of monitoring, product quality cannot obtain the monitoring of science, sample room that different manufacturers are produced and batch between quality
It differs greatly, causes clinical application Different therapeutical effect also larger.In order to improve the quality of wind-solar resources drug, to full side
The features such as having carried out quality determining method research, but identification be not readily separated because of the more complicated component of compound Chinese medicinal preparation taste of traditional Chinese medicine,
It is only capable of setting up individual components in the strong method for qualitative and quantitative detection other side of several specificities to be monitored, although prior art wind
Detection method (the patent No. of cold cold granules：CN 103267824B) in other side's epheday intermedia, campanulaceae carried out qualitative and quantitative inspection
It surveys, but can only reflect the quality condition of preparation epheday intermedia, campanulaceae ingredient, the total quality feelings of drug can not be embodied
Condition.
To in the present invention we HPLC characteristic spectrum experimental studies have been carried out to wind-solar resources, establish characteristic spectrum
Quality determining method, and to institute's method for building up carried out the methods of precision, repeatability learn verification test, the results showed that this hair
Bright middle detection method is stablized feasible.The present invention overcomes lack quality determining method in wind-solar resources now execution standard
Deficiency, and detection method can only reflect the one-sidedness of few drug ingedient quality condition, the characteristic pattern of foundation in the prior art
Spectrum assay method is effective supplement of wind-solar resources method of quality control, can reflect the type sum number of contained chemical composition
Amount, its quality of energy Efficient Characterization are conducive to the quality for controlling product comprehensively.
Invention content
The object of the present invention is to provide the construction method of the HPLC characteristic spectrums of Chinese patent drug wind-solar resources, this method packets
Include following steps：
(1) preparation of reference solution：Take Puerarin reference substance appropriate, it is accurately weighed, add 30~100% methanol to be made often
1ml contains the solution of 40~60 μ g.
(2) preparation of test solution:It takes wind-solar resources finely ground, takes about 2~5g, accurately weighed, precision is added 30
~100% 50~100ml of methanol, weighed weight are ultrasonically treated 30~60 minutes, wait for that solution is cooled to room temperature, with 30~100%
Methanol mends weight, shakes up, and is filtered with 0.22~0.45 μm of miillpore filter, take subsequent filtrate to get.
(3) chromatographic condition：Using ZORBAX SB-C18 chromatographic columns, (column length 250mm, internal diameter 4.6mm, grain size are 5 μ
m)；It is 3~70 that mobile phase, which is with -0.1% phosphoric acid solution of acetonitrile,：97~30 mixed solution；0.5~1.5ml/ of volume flow
min；DAD detectors, wavelength are 190~250nm；25~45 DEG C of column temperature, number of theoretical plate are not less than 10000.
(4) it measures：It is accurate respectively to draw reference solution and each 5~20 μ l of test solution, liquid chromatograph is injected, is shone
High effective liquid chromatography for measuring obtains the HPLC characteristic spectrums of wind-solar resources.
The best construction method of the HPLC characteristic spectrums of the present invention is as follows：(each substance described in technical solution once
Dosage or amount ranges, it is intended to show the usage ratio relationship between each substance, not be limited in the particular number of record)
Preferably, step (1) described reference solution is a concentration of contains 50 μ g Puerarin reference substances per 1ml50% methanol.
Preferably, the preparation process of step (2) described test solution is as follows：It takes wind-solar resources finely ground, takes about 2g,
Accurately weighed, 50% methanol 25ml is added in precision, and weighed weight is ultrasonically treated 30 minutes, lets cool, less loss is supplied with 50% methanol
Weight, shake up, filtered with 0.45 μm of miillpore filter, take subsequent filtrate to get.
Preferably, step (3) described mobile phase be using acetonitrile as mobile phase A, using 0.1% phosphoric acid solution as Mobile phase B, ladder
Degree elution.The time of gradient elution and mobile phase ratio are：0~20min, A phase：3%, B phases：97%；20~35min, A phase：3
~9%, B phases：97~91%；35~60min, A phase：9~19%, B phases：91~81%；60~85min, A phase：19~50%,
B phases：81~50%；85~90min, A phase：50~70%, B phases：50~30%；90~100min, A phase：70~3%, B phases：
30~97%；100~110min, A phase：3%, B phases：97%.
The HPLC characteristic spectrums that 15 batches of Chinese patent drug wind-solar resources are built according to preceding method, using Chinese medicine chromatographic fingerprint
Collection of illustrative plates similarity evaluation system software generates the compare feature collection of illustrative plates being made of 8 shared peaks, and the shared coneincone 2 is hydrochloric acid
Ephedrine peak, peak 3 are pseudoephedrine hydrochloride peak, and peak 4 is puerarin peak, and peak 7 is macrotin glycosides peak, and peak 8 is aurantiamarin peak.
It is to calculate the relative retention time of each characteristic peak and the peaks S, institute with reference to peak S with Puerarin in compare feature collection of illustrative plates
Relative retention time is stated within ± the 5% of the first specified value, first specified value is：The peaks 0.37- 1, the peaks 0.53- 2,
The peaks 0.58- 3, the peaks the 1.00- peak S, 1.03- 5, the peaks 1.05- 6, the peaks 1.11- 7, the peaks 1.39- 8；
Wind-solar resources are taken, are operated as stated above, wind-solar resources sample characteristic collection of illustrative plates are obtained, using Chinese medicine color
Spectrum fingerprint similarity evaluation software analyzes sample collection of illustrative plates and compare feature collection of illustrative plates, and similarity is more than 0.90 for qualification
Product.
Beneficial effects of the present invention are as follows：
(1) with method provided by the present invention can effectively monitor different batches between wind-solar resources quality, method
Have the characteristics that precision height, favorable reproducibility, is conducive to the quality of overall monitor product.
(2) quality determining method of the invention is with ephedrine hydrochloride, pseudoephedrine hydrochloride, Puerarin, cimicifugoside, aurantiamarin
Wind-solar resources characteristic spectrum for control, foundation focuses on the sequence and relationship of each characteristic peak, energy overall monitor product
Total quality facial feature, methodological science are reliable.
(3) best UV absorption wavelength of the invention is 210nm.Using method in the present invention with DAD detectors to chill
Cold granules are measured, and the Detection wavelength result for occurring absorption maximum according to all chromatographic peaks in 3-D scanning figure is transferred respectively
Chromatogram under each wavelength, when Detection wavelength is 210nm, characteristic peak number is more, between each peak area is higher and each characteristic peak
Separating effect is preferably also.
Description of the drawings
Fig. 1 is wind-solar resources HPLC characteristic spectrums
Fig. 2 is 15 batch HPLC characteristic spectrum stacking charts of wind-solar resources
Fig. 3 is wind-solar resources compare feature collection of illustrative plates
Attached drawing is explained：Peak 2 is ephedrine hydrochloride peak in Fig. 1, and peak 3 is pseudoephedrine hydrochloride peak, and peak 4 is puerarin peak, peak 7
For macrotin glycosides peak, peak 8 is aurantiamarin peak.
Specific implementation mode
Embodiment 1 establishes wind-solar resources HPLC characteristic spectrum assay methods
1 instrument：UltiMate 3000DGLC high performance liquid chromatographs, ultraviolet DAD detectors；Mettler AE240 100,000
/ mono- assay balance (Shanghai plum Teller-support benefit Instrument Ltd.)；AB204-E electronic balances (Shanghai plum Teller-Tuo Li
Multiple instruments Co., Ltd) etc..
2 reagents and reagent
2.1 reagent：Methanol etc. is that analysis is pure, and phosphoric acid, acetonitrile are chromatographically pure, and water is ultra-pure water.
2.2 reagent：Ephedrine hydrochloride, pseudoephedrine hydrochloride, Puerarin, cimicifugoside, aurantiamarin (are all from Chinese food medicine
Research institute is determined in tasting).
3 methods and result
The preparation of 3.1 reference solutions：Take Puerarin reference substance appropriate, it is accurately weighed, add 50% methanol that every 1ml is made and contains
The solution of 50 μ g.
The preparation of 3.2 test solutions:It takes wind-solar resources finely ground, takes about 2g, accurately weighed, precision, which adds, takes 50% first
Alcohol 25ml, weighed weight are ultrasonically treated 30 minutes, let cool, the weight of less loss is supplied with 50% methanol, is shaken up, micro- with 0.45 μm
The filtration of hole filter membrane, take subsequent filtrate to get.
3.3 chromatographic condition：Using ZORBAX SB-C18 chromatographic columns, (column length 250mm, internal diameter 4.6mm, grain size are 5 μ
m)；Mobile phase be using acetonitrile as mobile phase A, using 0.1% phosphoric acid solution as Mobile phase B, gradient elution；Volume flow 1.0mL/
min；DAD detectors, wavelength 210nm；35 DEG C of column temperature；10 μ L of sample size.Number of theoretical plate is not less than 10000.
The time of gradient elution and mobile phase ratio are：0~20min, A phase：3%, B phases：97%；20~35min, A phase：
3~9%, B phases：97~91%；35~60min, A phase：9~19%, B phases：91~81%；60~85min, A phase：19~
50%, B phases：81~50%；85~90min, A phase：50~70%, B phases：50~30%；90~100min, A phase：70~3%,
B phases：30~97%；100~110min, A phase：3%, B phases：97%.
3.4 measuring：It is accurate respectively to draw test solution and each 10 μ l of reference solution, liquid chromatograph is injected, according to height
Effect liquid phase chromatogram method measures, and obtains characteristic spectrum, as shown in Figure 1.
4. wind-solar resources HPLC characteristic spectrum characteristic peaks are pointed out
Take ephedrine hydrochloride, pseudoephedrine hydrochloride, Puerarin, cimicifugoside, aurantiamarin reference substance appropriate, it is accurate respectively to claim
It is fixed, add 50% methanol that hydrochloric 90 μ g of ephedrine of every 1ml, 90 μ g of pseudoephedrine hydrochloride, 50 μ g of Puerarin, 50 μ g of cimicifugoside is made
With the solution of 100 μ g of aurantiamarin, measure in accordance with the law.Chromatographic peak is pointed out by above-mentioned reference substance, peak 2 is ephedrine hydrochloride
Peak, peak 3 are pseudoephedrine hydrochloride peak, and peak 4 is puerarin peak, and peak 7 is macrotin glycosides peak, and peak 8 is aurantiamarin peak.
5. wind-solar resources characteristic spectrum mensuration methodology is investigated
5.1 precision test：Wind-solar resources are taken, test solution, continuous sample introduction 5 times is made by step (2) method.
As a result：5 equal ﹤ 2% of relative retention time RSD values for measuring each shared peak in characteristic spectrum, show that instrument precision is good.
5.2 stability test：Take wind-solar resources, test solution be made by step (2) method, respectively 0,2,4,
8, it is detected in accordance with the law within 12 hours.As a result：5 equal ﹤ 2% of relative retention time RSD values for measuring each shared peak in characteristic spectrum,
Show that test solution is stablized between 12 hours.
5.3 repetitive test：It takes with a collection of wind-solar resources, 6 parts of test solutions is prepared by step (2) method, according to
Method measures.As a result：6 equal ﹤ 2% of relative retention time RSD values for measuring each shared peak in characteristic spectrum, show the repetition of method
Property is preferable.
The compare feature collection of illustrative plates of 6 structure wind-solar resources
6.1 take wind-solar resources 15 batches, are measured by the condition of embodiment 1, obtain 15 batch sample HPLC features
Collection of illustrative plates, as shown in Figure 2.It is compared, is determined using 15 batches of characteristic spectrums of chromatographic fingerprints of Chinese materia medica similarity evaluation software pair
Common characteristic peaks generate the compare feature collection of illustrative plates being made of 8 shared peaks, as shown in Figure 3.It is wherein puerarin peak (4 with reference to peak
Number peak).
6.2 similarity analysis:It is analyzed with chromatographic fingerprints of Chinese materia medica similarity evaluation software, calculates 15 batches of test sample features
The similarity of collection of illustrative plates and the compare feature collection of illustrative plates of generation, is as a result all higher than 0.90.Similarity-rough set the results are shown in Table 1.
Table 1HPLC characteristic spectrum similarity evaluation results
| Sample batch | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 |
| Similarity | 1 | 0.9 45 | 0.9 21 | 0.9 97 | 0.9 88 | 0.9 75 | 0.9 21 | 0.9 15 | 0.9 63 | 0.9 77 | 0.9 15 | 0.9 92 | 0.9 83 | 0.9 41 | 0.9 93 |
The quality determining method of 7 wind-solar resources
7.1 take 10 batches of wind-solar resources preparations (by tetra- different manufacturers productions of A, B, C, D), by the condition of embodiment 1
It is measured, obtains the HPLC characteristic spectrums of 10 batch samples, using chromatographic fingerprints of Chinese materia medica similarity evaluation software to sample
Product characteristic spectrum is analyzed with compare feature collection of illustrative plates, and it is qualified products that similarity, which is more than 0.90,.
7.2 testing results are that tri- 8 batch products of producer of A, B, D are certified products, and 2 batch products of C producers are defective work.
In conclusion the stability of quality determining method constructed by the present invention is good, precision is high, reproducible, therefore this
Invention incidence of criminal offenses has feasibility.Obtained Chinese patent drug wind-solar resources, quality are monitored using quality determining method of the present invention
Unanimously, curative effect is stablized.
Claims (6)
1. a kind of construction method of wind-solar resources HPLC characteristic spectrums, it is characterized in that：Include the following steps：
(1) preparation of reference solution：Take Puerarin reference substance appropriate, it is accurately weighed, add 30~100% methanol that every 1ml is made
Solution containing 40~60 μ g；
(2) preparation of test solution：It takes wind-solar resources finely ground, takes 2~5g, accurately weighed, precision is added 30~100%
Methanol 25ml or 50~100ml, weighed weight are ultrasonically treated 30~60 minutes, let cool, less loss is supplied with 30~100% methanol
Weight, shake up, filtered with 0.22~0.45 μm of miillpore filter, take subsequent filtrate to get；
(3) chromatographic condition：Using ZORBAX SB-C18 chromatographic columns, the column length of the chromatographic column is 250mm, internal diameter 4.6mm,
Grain size is 5 μm；Mobile phase be using acetonitrile as mobile phase A, using 0.1% phosphoric acid solution as Mobile phase B, gradient elution, gradient elution
Time and mobile phase ratio be：0~20min, A phase：3%, B phases：97%；20~35min, A phase：3~9%, B phases：97~
91%；35~60min, A phase：9~19%, B phases：91~81%；60~85min, A phase：19~50%, B phases：81~50%；
85~90min, A phase：50~70%, B phases：50~30%；90~100min, A phase：70~3%, B phases：30~97%；100~
110min, A phase：3%, B phases：97%；0.5~1.5ml/min of volume flow；DAD detectors, wavelength are 190~250nm；Column
25~45 DEG C of temperature, number of theoretical plate are not less than 10000；
(4) it measures：It is accurate respectively to draw reference solution and each 5~20 μ l of test solution, liquid chromatograph is injected, according to efficient
Liquid chromatography for measuring obtains the HPLC characteristic spectrums of wind-solar resources.
2. a kind of construction method of wind-solar resources HPLC characteristic spectrums according to claim 1, it is characterized in that：
Step (1) described reference solution is a concentration of to contain 50 μ g Puerarin reference substances per 50% methanol of 1ml.
3. a kind of construction method of wind-solar resources HPLC characteristic spectrums according to claim 1, it is characterized in that：
The preparation process of step (2) described test solution is as follows：It takes wind-solar resources finely ground, takes 2g, it is accurately weighed, it is accurate
50% methanol 25ml is added, weighed weight is ultrasonically treated 30 minutes, lets cool, the weight of less loss is supplied with 50% methanol, is shaken up,
Filtered with 0.45 μm of miillpore filter, take subsequent filtrate to get.
4. a kind of construction method of wind-solar resources HPLC characteristic spectrums according to claim 1, it is characterized in that：
Step (4) builds the HPLC characteristic spectrums of 15 batches of Chinese patent drug wind-solar resources according to preceding method, using Chinese medicine chromatography
The compare feature collection of illustrative plates that fingerprint similarity evaluation system Software Create is made of 8 shared peaks, the shared coneincone 2 are
Ephedrine hydrochloride peak, peak 3 are pseudoephedrine hydrochloride peak, and peak 4 is puerarin peak, and peak 7 is macrotin glycosides peak, and peak 8 is aurantiamarin peak.
5. a kind of construction method of wind-solar resources HPLC characteristic spectrums according to claim 1, it is characterized in that：Also wrap
It includes：
Step (5) takes wind-solar resources, operates as stated above, wind-solar resources sample characteristic collection of illustrative plates is obtained, in
Medicine chromatographic fingerprinting similarity evaluation software analyzes sample collection of illustrative plates and compare feature collection of illustrative plates, and similarity is more than 0.90
Qualified products.
6. a kind of construction method of wind-solar resources HPLC characteristic spectrums according to claim 5, it is characterized in that：Right
It is to calculate the relative retention time of each characteristic peak and the peaks S with reference to peak S with Puerarin according in characteristic spectrum, when the opposite reservation
Between within ± the 5% of the first specified value, first specified value is：The peaks 0.37- 1, the peaks 0.53- 2, the peaks 0.58- 3, the peaks 1.00-
S, the peaks 1.03- 5, the peaks 1.05- 6, the peaks 1.11- 7, the peaks 1.39- 8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610947350.8A CN106645459B (en) | 2016-10-26 | 2016-10-26 | A kind of construction method of wind-solar resources HPLC characteristic spectrums |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610947350.8A CN106645459B (en) | 2016-10-26 | 2016-10-26 | A kind of construction method of wind-solar resources HPLC characteristic spectrums |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106645459A CN106645459A (en) | 2017-05-10 |
| CN106645459B true CN106645459B (en) | 2018-10-30 |
Family
ID=58821310
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610947350.8A Active CN106645459B (en) | 2016-10-26 | 2016-10-26 | A kind of construction method of wind-solar resources HPLC characteristic spectrums |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106645459B (en) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114778710A (en) * | 2022-02-21 | 2022-07-22 | 吉林修正药业新药开发有限公司 | HPLC (high Performance liquid chromatography) characteristic map construction method of forsythia suspense toxin-vanquishing tablets and method for monitoring quality of forsythia suspense toxin-vanquishing tablets |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103760272A (en) * | 2014-01-27 | 2014-04-30 | 山东齐都药业有限公司 | Method for constructing feature fingerprint spectrum of rhizoma gastrodiae and ligusticum wallichii particle product |
| CN104215702A (en) * | 2013-06-04 | 2014-12-17 | 吉林康乃尔药业有限公司 | Salvia miltiorrhiza snakegourd peel tablet fingerprint detection method |
| CN104237444A (en) * | 2014-10-15 | 2014-12-24 | 辽宁中医药大学 | Construction method of HPLC (High Performance Liquid Chromatography) fingerprint spectrum of salvia miltiorrhiza and radix puerariae depression relieving drug |
| CN105758978A (en) * | 2016-04-25 | 2016-07-13 | 广西壮族自治区梧州食品药品检验所 | Method for measuring contents of various ingredients in cold cough syrup |
| CN105842353A (en) * | 2016-03-18 | 2016-08-10 | 江苏康缘药业股份有限公司 | Establishing method of fingerprint spectrum of honeysuckle-fructus forsythiae heat-clearing tablets and fingerprint spectrum |
-
2016
- 2016-10-26 CN CN201610947350.8A patent/CN106645459B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104215702A (en) * | 2013-06-04 | 2014-12-17 | 吉林康乃尔药业有限公司 | Salvia miltiorrhiza snakegourd peel tablet fingerprint detection method |
| CN103760272A (en) * | 2014-01-27 | 2014-04-30 | 山东齐都药业有限公司 | Method for constructing feature fingerprint spectrum of rhizoma gastrodiae and ligusticum wallichii particle product |
| CN104237444A (en) * | 2014-10-15 | 2014-12-24 | 辽宁中医药大学 | Construction method of HPLC (High Performance Liquid Chromatography) fingerprint spectrum of salvia miltiorrhiza and radix puerariae depression relieving drug |
| CN105842353A (en) * | 2016-03-18 | 2016-08-10 | 江苏康缘药业股份有限公司 | Establishing method of fingerprint spectrum of honeysuckle-fructus forsythiae heat-clearing tablets and fingerprint spectrum |
| CN105758978A (en) * | 2016-04-25 | 2016-07-13 | 广西壮族自治区梧州食品药品检验所 | Method for measuring contents of various ingredients in cold cough syrup |
Non-Patent Citations (3)
| Title |
|---|
| HPLC-DAD法测定冠脉宁片中葛根素和丹酚酸B的量及其指纹图谱研究;丁宁 等;《首都医科大学学报》;20151231;第36卷(第6期);第958-963页 * |
| 华盖散制剂-药材谱峰匹配指纹图谱研究;董自亮 等;《中草药》;20160229;第47卷(第3期);第425-429页 * |
| 复方龙脉宁HPLC指纹图谱的研究;王莎 等;《中华中医药学刊》;20150131;第33卷(第1期);第89-92页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106645459A (en) | 2017-05-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105891353B (en) | A kind of method for building up and its finger-print of Tanreqing capsule fingerprint pattern | |
| CN105486771B (en) | The fingerprint atlas detection method of Xiao Chai Hu granules compound preparation | |
| CN105842353B (en) | The method for building up and its finger-print of Lonicera and Forsythia heat clearing tablet finger-print | |
| CN110031570B (en) | Fingerprint spectrum detection method of anti-cold granules | |
| CN109633001B (en) | Fingerprint detection method of wine glossy privet fruit | |
| CN110736799B (en) | Quality detection method of traditional Chinese medicine children's cold-relieving granules | |
| CN106290646B (en) | The detection method of Compound Jinyinhua Granules | |
| CN106950324B (en) | A kind of Radix Astragali builds the construction method of middle ball finger-print | |
| CN106483213A (en) | The method for building up of Taohe Chengqi decoction composition finger-print and finger-print | |
| CN106645508A (en) | Construction method of HPLC specific chromatogram of wind-heat common cold granule | |
| CN105628834B (en) | Anaesthetic Radix Rhapontici seu Radix Echinopsis spends fingerprint and quality evaluating method | |
| CN106645459B (en) | A kind of construction method of wind-solar resources HPLC characteristic spectrums | |
| CN109765322A (en) | The characteristic spectrum construction method and quality determining method of schizonepeta | |
| CN111487347B (en) | Method for detecting fingerprint of Zhishu granules | |
| CN1969953A (en) | Quality control method of honeysuckle, scutellarta root and extract thereof and formulation containing the extract | |
| CN110568108B (en) | Multi-component content determination method of Ganfule preparation | |
| CN110441413B (en) | Construction method and detection method of HPLC fingerprint of Qianbai rhinitis tablets | |
| CN102133333A (en) | Quality control method for shenmai injection mass spectrum finger prints | |
| CN110031564B (en) | Quality detection method of natural plant anticoccidial feed additive based on HPLC fingerprint | |
| CN110274970A (en) | The method for building up for melting poor finger-print and its application in Yixiesheng capsule Quality Control | |
| CN110297047B (en) | Establishment method of HPLC fingerprint of lysimachia capillipes hemsl medicinal material and fingerprint thereof | |
| CN104807932A (en) | Determining method of fingerprint spectrum of flavonoid ingredient of fenugreek seed extract | |
| CN103487528A (en) | HPLC fingerprint determination method of cough relieving Bulbus fritillariae cirrhosae and loquat dripping pills | |
| CN108562681A (en) | The quantitative detection method of QIJU DIHUANG KOUFUYE multicomponent | |
| CN111060637B (en) | Quality control method of agilawood koji |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190620 Address after: 130000 1369 Shunda Road, Changchun, Jilin Co-patentee after: Jilin Changbaishan Pharmaceutical Group Co., Ltd. Patentee after: Jilin xiuzheng pharmaceutical new medicine development Co., Ltd. Address before: 130000 1369 Shunda Road, Changchun, Jilin Patentee before: Jilin xiuzheng pharmaceutical new medicine development Co., Ltd. |