CN106645459A - Method for constructing HPLC specific chromatogram of granules for treating common cold with wind-cold syndrome - Google Patents
Method for constructing HPLC specific chromatogram of granules for treating common cold with wind-cold syndrome Download PDFInfo
- Publication number
- CN106645459A CN106645459A CN201610947350.8A CN201610947350A CN106645459A CN 106645459 A CN106645459 A CN 106645459A CN 201610947350 A CN201610947350 A CN 201610947350A CN 106645459 A CN106645459 A CN 106645459A
- Authority
- CN
- China
- Prior art keywords
- wind
- peak
- phase
- peaks
- phases
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
Landscapes
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention provides a method for constructing HPLC specific chromatogram of granules for treating common cold with wind-cold syndrome. The method comprises the following steps: (1) preparing a reference solution, namely weighing a proper amount of a puerarin reference substance, and precisely weighing; (2) preparing a test sample solution, namely porphyrizing granules for treating common cold with wind-cold syndrome, weighing about 2-5g, and precisely weighing; (3) determining chromatographic conditions, namely adopting ZORBAX SB-C18 chromatographic column, a mixed solution prepared from acetonitrile-0.1%phosphoric acid in a ratio of (3-70):(97-30) as a mobile phase, a volume flow of 0.5-1.5ml/min, a DAD detector having a wave length of 190-250nm, a column temperature of 25-45 DEG C, and the number of theoretical plates of not lower than 10000; and (4) detecting, namely respectively precisely drawing 5-20mu l of the reference solution and the test sample solution, injecting into a liquid chromatograph, and detecting according to a high-performance liquid chromatography method to obtain an HPLC specific chromatogram of granules for treating common cold with wind-cold syndrome. The method has the advantage of effectively monitoring the quality of granules for treating common cold with wind-cold syndrome in different batches, has the characteristics of high precision, good reproducibility and the like, and is favorable to comprehensive monitoring of product quality.
Description
Technical field
The invention belongs to field of medicaments, and in particular to the construction method of Chinese patent medicine wind-solar resources HPLC characteristic spectrums.
Background technology
Wind-solar resources are by Herba Ephedrae, Radix Puerariae, Folium Perillae, Radix Saposhnikoviae, Ramulus Cinnamomi, the Radix Angelicae Dahuricae, Pericarpium Citri Reticulatae, Semen Armeniacae Amarum, Radix Platycodoniss, sweet
Simply Chinese herbal medicine is constituted grass, Rhizoma Zingiberiss etc. ten.Major function is relieving the exterior syndrome by diaphoresis, expelling wind and cold.For fever of the body of catching a cold, headache, cough,
The diseases such as nasal obstruction, watery nasal discharge.Existing implementing quality standards are recorded in Chinese patent medicine ministry standard, and standard No. is WS3-B-0045-89, in standard
The method of quality control such as uncharged indentification by TLC and assay.Because intermediate not to product, Cheng Kou carry out matter
The effective means of amount monitoring, product quality can not obtain the monitoring of science, sample room that different manufacturers are produced and batch between quality
Differ greatly, cause clinical practice Different therapeutical effect also larger.In order to improve the quality of wind-solar resources medicine, to full side
Carried out quality determining method research, but because the more complicated component of compound Chinese medicinal preparation taste of Chinese medicine be not readily separated identification the features such as,
It is only capable of setting up individual components in the strong method for qualitative and quantitative detection other side of several specificities and is monitored, although prior art wind
Detection method (the patent No. of cold cold granules：CN 103267824B) in other side's epheday intermedia, Radix Platycodoniss carried out qualitative and quantitative inspection
Survey, but preparation epheday intermedia, the quality condition of Radix Platycodoniss ingredient can only be reflected, the total quality feelings of medicine can not be embodied
Condition.
We have carried out HPLC characteristic spectrum experimental studies to wind-solar resources in the present invention, establish characteristic spectrum
Quality determining method, and institute's method for building up has been carried out the Method validations such as precision, repeatability test, as a result show this
Bright middle detection method stablizes feasible.Instant invention overcomes wind-solar resources lack quality determining method in execution standard now
Detection method can only reflect the one-sidedness of few ingredient quality condition, the characteristic pattern of foundation in deficiency, and prior art
Spectrum assay method is effective supplement of wind-solar resources method of quality control, can reflect the species sum of contained chemical composition
Amount, can Efficient Characterization its quality, be conducive to controlling the quality of product comprehensively.
The content of the invention
It is an object of the invention to provide the construction method of the HPLC characteristic spectrums of Chinese patent medicine wind-solar resources, the method bag
Include following steps：
(1) preparation of reference solution：Take puerarin reference substance appropriate, it is accurately weighed, plus 30～100% methanol makes often
Solution of the 1ml containing 40～60 μ g.
(2) preparation of need testing solution:Take wind-solar resources finely ground, take about 2～5g, accurately weighed, precision adds 30
～100% 50～100ml of methanol, weighed weight, supersound process 30～60 minutes treats that solution is cooled to room temperature, with 30～100%
Methanol mends weight, shakes up, and is filtered with 0.22～0.45 μm of microporous filter membrane, takes subsequent filtrate, obtains final product.
(3) chromatographic condition：(column length is 250mm, and internal diameter is 4.6mm, and particle diameter is 5 μ to adopt ZORBAX SB-C18 chromatographic columns
m)；Mobile phase is with the phosphoric acid solution of acetonitrile -0.1% as 3～70：97～30 mixed solution；0.5～1.5ml/ of volume flow
min；DAD detectors, wavelength is 190～250nm；25～45 DEG C of column temperature, number of theoretical plate is not less than 10000.
(4) determine：It is accurate respectively to draw reference solution and each 5～20 μ l of need testing solution, chromatograph of liquid is injected, shine
High effective liquid chromatography for measuring, obtains the HPLC characteristic spectrums of wind-solar resources.
The optimal construction method of the HPLC characteristic spectrums of the present invention is as follows：(once each material described in technical scheme
Consumption or amount ranges, it is intended to show the usage ratio relation between each material, be not limited in the particular number of record)
Preferably, step (1) the reference solution concentration is that every 1ml50% methanol contains 50 μ g puerarin reference substances.
Preferably, the preparation process of step (2) need testing solution is as follows：Take wind-solar resources finely ground, take about 2g,
Accurately weighed, precision adds 50% methanol 25ml, weighed weight, supersound process 30 minutes to let cool, and with 50% methanol less loss is supplied
Weight, shake up, filtered with 0.45 μm of microporous filter membrane, take subsequent filtrate, obtain final product.
Preferably, step (3) mobile phase be with acetonitrile as mobile phase A, with 0.1% phosphoric acid solution as Mobile phase B, ladder
Degree eluting.The time of gradient elution and mobile phase ratio are：0～20min, A phase：3%th, B phases：97%；20～35min, A phase：3
～9%, B phases：97～91%；35～60min, A phase：9～19%, B phases：91～81%；60～85min, A phase：19～50%,
B phases：81～50%；85～90min, A phase：50～70%, B phases：50～30%；90～100min, A phase：70～3%, B phases：
30～97%；100～110min, A phase：3%th, B phases：97%.
The HPLC characteristic spectrums of 15 batches of Chinese patent medicine wind-solar resources are built according to preceding method, using Chinese medicine chromatographic fingerprint
Collection of illustrative plates similarity evaluation systems soft ware generates the compare feature collection of illustrative plates being made up of 8 total peaks, and the total coneincone 2 is hydrochloric acid
Ephedrine peak, peak 3 is pseudoephedrine hydrochloride peak, and peak 4 is puerarin peak, and peak 7 is cimicifugoside peak, and peak 8 is Hesperidin peak.
It is reference peak S with puerarin in compare feature collection of illustrative plates, calculates the relative retention time of each characteristic peak and S peaks, institute
Relative retention time is stated within ± the 5% of the first setting, first setting is：0.37- peaks 1,0.53- peaks 2,
0.58- peaks 3,1.00- peaks S, 1.03- peak 5,1.05- peaks 6,1.11- peaks 7,1.39- peaks 8；
Wind-solar resources are taken, is operated as stated above, wind-solar resources sample characteristic collection of illustrative plates is obtained, using Chinese medicine color
Spectrum fingerprint similarity evaluation software is analyzed to sample collection of illustrative plates with compare feature collection of illustrative plates, and similarity is qualified more than 0.90
Product.
Beneficial effects of the present invention are as follows：
(1) quality of wind-solar resources between different batches, method can be effectively monitored with method provided by the present invention
The features such as with precision height, favorable reproducibility, be conducive to the quality of overall monitor product.
(2) quality determining method of the invention is with ephedrine hydrochloride, pseudoephedrine hydrochloride, puerarin, cimicifugoside, Hesperidin
To compare, the wind-solar resources characteristic spectrum of foundation focuses on the order and relation of each characteristic peak, energy overall monitor product
Total quality facial feature, methodological science reliability.
(3) optimal uv absorption wavelength of the invention is 210nm.Using method in the present invention with DAD detectors to wind and cold
Cold granules are measured, and are transferred respectively according to the Detection wavelength result that absorption maximum occur in all chromatographic peaks in 3-D scanning figure
Chromatogram under each wavelength, when Detection wavelength is 210nm, characteristic peak number is more, each peak area is higher and each characteristic peak between
Separating effect is preferably also.
Description of the drawings
Fig. 1 is wind-solar resources HPLC characteristic spectrums
Fig. 2 is the batch HPLC characteristic spectrum stacking chart of wind-solar resources 15
Fig. 3 is wind-solar resources compare feature collection of illustrative plates
Accompanying drawing is explained：In Fig. 1 peak 2 be ephedrine hydrochloride peak, peak 3 be pseudoephedrine hydrochloride peak, peak 4 be puerarin peak, peak 7
For cimicifugoside peak, peak 8 is Hesperidin peak.
Specific embodiment
Embodiment 1 sets up wind-solar resources HPLC characteristic spectrum assay methods
1 instrument：UltiMate 3000DGLC high performance liquid chromatographs, ultraviolet DAD detectors；Mettler AE240 100,000
/ mono- analytical balance (Shanghai prunus mume (sieb.) sieb.et zucc. Teller-support benefit Instrument Ltd.)；AB204-E electronic balances (Shanghai prunus mume (sieb.) sieb.et zucc. Teller-Tuo Li
Multiple instruments company limited) etc..
2 reagents and reagent
2.1 reagent：Methanol etc. is pure for analysis, and phosphoric acid, acetonitrile are chromatographically pure, and water is ultra-pure water.
2.2 reagent：Ephedrine hydrochloride, pseudoephedrine hydrochloride, puerarin, cimicifugoside, Hesperidin (are all from Chinese food medicine
Academy is determined in tasting).
3 methods and result
The preparation of 3.1 reference solutions：Take puerarin reference substance appropriate, it is accurately weighed, plus 50% methanol makes every 1ml and contains
The solution of 50 μ g.
The preparation of 3.2 need testing solutions:Take wind-solar resources finely ground, take about 2g, it is accurately weighed, precision plus take 50% first
Alcohol 25ml, weighed weight, supersound process 30 minutes lets cool, and with 50% methanol the weight of less loss is supplied, and shakes up, micro- with 0.45 μm
Hole filter membrane filtration, takes subsequent filtrate, obtains final product.
3.3 chromatographic condition：(column length is 250mm, and internal diameter is 4.6mm, and particle diameter is 5 μ to adopt ZORBAX SB-C18 chromatographic columns
m)；Mobile phase be with acetonitrile as mobile phase A, with 0.1% phosphoric acid solution as Mobile phase B, gradient elution；Volume flow 1.0mL/
min；DAD detectors, wavelength is 210nm；35 DEG C of column temperature；The μ L of sample size 10.Number of theoretical plate is not less than 10000.
The time of gradient elution and mobile phase ratio are：0～20min, A phase：3%th, B phases：97%；20～35min, A phase：
3～9%, B phases：97～91%；35～60min, A phase：9～19%, B phases：91～81%；60～85min, A phase：19～
50%th, B phases：81～50%；85～90min, A phase：50～70%, B phases：50～30%；90～100min, A phase：70～3%,
B phases：30～97%；100～110min, A phase：3%th, B phases：97%.
3.4 determine：It is accurate respectively to draw need testing solution and each 10 μ l of reference solution, chromatograph of liquid is injected, according to height
Effect liquid phase chromatogram method is determined, and obtains characteristic spectrum, as shown in Figure 1.
4. wind-solar resources HPLC characteristic spectrums characteristic peak is pointed out
Take ephedrine hydrochloride, pseudoephedrine hydrochloride, puerarin, cimicifugoside, Hesperidin reference substance appropriate, it is accurate respectively to claim
Determine, plus 50% methanol makes the hydrochloric μ g of ephedrine 90 of every 1ml, the μ g of pseudoephedrine hydrochloride 90, the μ g of puerarin 50, the μ g of cimicifugoside 50
With the solution of the μ g of Hesperidin 100, determine in accordance with the law.Chromatographic peak is pointed out by above-mentioned reference substance, peak 2 is ephedrine hydrochloride
Peak, peak 3 is pseudoephedrine hydrochloride peak, and peak 4 is puerarin peak, and peak 7 is cimicifugoside peak, and peak 8 is Hesperidin peak.
5. wind-solar resources characteristic spectrum mensuration methodology is investigated
5.1 precision test：Wind-solar resources are taken, by step (2) method need testing solution, continuous sample introduction 5 times are made.
As a result：The equal ﹤ 2% of relative retention time RSD value for determining each total peak in characteristic spectrum 5 times, shows that instrument precision is good.
5.2 stability test：Take wind-solar resources, by step (2) method need testing solution made, respectively 0,2,4,
8th, detected in accordance with the law within 12 hours.As a result：The equal ﹤ 2% of relative retention time RSD value for determining each total peak in characteristic spectrum 5 times,
Show that need testing solution is stable between 12 hours.
5.3 replica test：Take with a collection of wind-solar resources, by step (2) method 6 parts of need testing solutions are prepared, according to
Method is determined.As a result：The equal ﹤ 2% of relative retention time RSD value for determining each total peak in characteristic spectrum 6 times, shows the repetition of method
Property is preferable.
The 6 compare feature collection of illustrative plates for building wind-solar resources
6.1 take wind-solar resources 15 batches, are measured by the condition of embodiment 1, obtain 15 batch sample HPLC features
Collection of illustrative plates, as shown in Figure 2.15 batches of characteristic spectrums are compared using chromatographic fingerprints of Chinese materia medica similarity evaluation software, it is determined that
Common characteristic peaks, generate the compare feature collection of illustrative plates being made up of 8 total peaks, as shown in Figure 3.It is puerarin peak (4 wherein with reference to peak
Number peak).
6.2 similarity analysis:With chromatographic fingerprints of Chinese materia medica similarity evaluation software analysis, 15 batches of test sample features are calculated
Collection of illustrative plates and the similarity of the compare feature collection of illustrative plates for generating, are as a result all higher than 0.90.Similarity-rough set the results are shown in Table 1.
Table 1HPLC characteristic spectrum similarity evaluation results
| Sample batch | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 |
| Similarity | 1 | 0.945 | 0.921 | 0.997 | 0.988 | 0.975 | 0.921 | 0.915 | 0.963 | 0.977 | 0.915 | 0.992 | 0.983 | 0.941 | 0.993 |
The quality determining method of 7 wind-solar resources
7.1 take 10 batches of wind-solar resources preparations (by tetra- different manufacturers productions of A, B, C, D), by the condition of embodiment 1
It is measured, obtains the HPLC characteristic spectrums of 10 batch samples, using chromatographic fingerprints of Chinese materia medica similarity evaluation software to sample
Product characteristic spectrum is analyzed with compare feature collection of illustrative plates, and similarity is qualified products more than 0.90.
It is certified products that 7.2 testing results are tri- batch products of producer 8 of A, B, D, and the batch products of C producers 2 are defective work.
In sum, the good stability of the constructed quality determining method of the present invention, precision is high, reproducible, therefore this
Invention incidence of criminal offenses has feasibility.Obtained Chinese patent medicine wind-solar resources, quality are monitored using quality determining method of the present invention
Unanimously, stable curative effect.
Claims (3)
1. a kind of construction method of wind-solar resources HPLC characteristic spectrums, is characterized in that：Comprise the steps：
(1) preparation of reference solution：Take puerarin reference substance appropriate, it is accurately weighed, plus 30～100% methanol makes every 1ml
Solution containing 40～60 μ g；
(2) preparation of need testing solution：Take wind-solar resources finely ground, take about 2～5g, it is accurately weighed, precision addition 30～
100% 50～100ml of methanol, weighed weight, supersound process 30～60 minutes lets cool, and with 30～100% methanol less loss is supplied
Weight, shakes up, and is filtered with 0.22～0.45 μm of microporous filter membrane, takes subsequent filtrate, obtains final product；
(3) chromatographic condition：Adopt ZORBAX SB-C18 chromatographic columns (column length is 250mm, and internal diameter is 4.6mm, and particle diameter is 5 μm)；Stream
Dynamic phase is with the phosphoric acid solution of acetonitrile -0.1% as 3～70：97～30 mixed solution；0.5～1.5ml/min of volume flow；DAD
Detector, wavelength is 190～250nm；25～45 DEG C of column temperature, number of theoretical plate is not less than 10000；
(4) determine：It is accurate respectively to draw reference solution and each 5～20 μ l of need testing solution, chromatograph of liquid is injected, according to efficient
Liquid chromatography for measuring, obtains the HPLC characteristic spectrums of wind-solar resources.
2. a kind of construction method of wind-solar resources HPLC characteristic spectrums according to claim 1, is characterized in that：Including
Following step：
Step (1) the reference solution concentration is that every 1ml50% methanol contains 50 μ g puerarin reference substances；
The preparation process of step (2) need testing solution is as follows：Take wind-solar resources finely ground, take about 2g, accurately weighed, essence
50% methanol 25ml of close addition, weighed weight, supersound process 30 minutes lets cool, and with 50% methanol the weight of less loss is supplied, and shakes
It is even, filtered with 0.45 μm of microporous filter membrane, subsequent filtrate is taken, obtain final product；
Step (3) mobile phase be with acetonitrile as mobile phase A, with 0.1% phosphoric acid solution as Mobile phase B, gradient elution, gradient
The time of eluting and mobile phase ratio are：0～20min, A phase：3%th, B phases：97%；20～35min, A phase：3～9%, B phases：
97～91%；35～60min, A phase：9～19%, B phases：91～81%；60～85min, A phase：19～50%, B phases：81～
50%；85～90min, A phase：50～70%, B phases：50～30%；90～100min, A phase：70～3%, B phases：30～97%；
100～110min, A phase：3%th, B phases：97%；
Step (4) builds the HPLC characteristic spectrums of 15 batches of Chinese patent medicine wind-solar resources according to preceding method, using Chinese medicine chromatograph
The compare feature collection of illustrative plates that fingerprint similarity evaluation system Software Create is made up of 8 total peaks, the total coneincone 2 is
Ephedrine hydrochloride peak, peak 3 is pseudoephedrine hydrochloride peak, and peak 4 is puerarin peak, and peak 7 is cimicifugoside peak, and peak 8 is Hesperidin peak；
Step (5) takes wind-solar resources, operates as stated above, wind-solar resources sample characteristic collection of illustrative plates is obtained, in employing
Medicine chromatographic fingerprinting similarity evaluation software is analyzed to sample collection of illustrative plates with compare feature collection of illustrative plates, and similarity is more than 0.90
Qualified products.
3. a kind of construction method of wind-solar resources HPLC characteristic spectrums according to claim 1, is characterized in that：Right
Take puerarin as the relative retention time that each characteristic peak and S peaks are calculated with reference to peak S, during the relative reservation according to characteristic spectrum
Between within ± the 5% of the first setting, first setting is：0.37- peaks 1,0.53- peaks 2,0.58- peaks 3,1.00- peaks
S, 1.03- peak 5,1.05- peaks 6,1.11- peaks 7,1.39- peaks 8.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610947350.8A CN106645459B (en) | 2016-10-26 | 2016-10-26 | A kind of construction method of wind-solar resources HPLC characteristic spectrums |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610947350.8A CN106645459B (en) | 2016-10-26 | 2016-10-26 | A kind of construction method of wind-solar resources HPLC characteristic spectrums |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106645459A true CN106645459A (en) | 2017-05-10 |
| CN106645459B CN106645459B (en) | 2018-10-30 |
Family
ID=58821310
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610947350.8A Active CN106645459B (en) | 2016-10-26 | 2016-10-26 | A kind of construction method of wind-solar resources HPLC characteristic spectrums |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106645459B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114778710A (en) * | 2022-02-21 | 2022-07-22 | 吉林修正药业新药开发有限公司 | HPLC (high Performance liquid chromatography) characteristic map construction method of forsythia suspense toxin-vanquishing tablets and method for monitoring quality of forsythia suspense toxin-vanquishing tablets |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103760272A (en) * | 2014-01-27 | 2014-04-30 | 山东齐都药业有限公司 | Method for constructing feature fingerprint spectrum of rhizoma gastrodiae and ligusticum wallichii particle product |
| CN104215702A (en) * | 2013-06-04 | 2014-12-17 | 吉林康乃尔药业有限公司 | Salvia miltiorrhiza snakegourd peel tablet fingerprint detection method |
| CN104237444A (en) * | 2014-10-15 | 2014-12-24 | 辽宁中医药大学 | Construction method of HPLC (High Performance Liquid Chromatography) fingerprint spectrum of salvia miltiorrhiza and radix puerariae depression relieving drug |
| CN105758978A (en) * | 2016-04-25 | 2016-07-13 | 广西壮族自治区梧州食品药品检验所 | Method for measuring contents of various ingredients in cold cough syrup |
| CN105842353A (en) * | 2016-03-18 | 2016-08-10 | 江苏康缘药业股份有限公司 | Establishing method of fingerprint spectrum of honeysuckle-fructus forsythiae heat-clearing tablets and fingerprint spectrum |
-
2016
- 2016-10-26 CN CN201610947350.8A patent/CN106645459B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104215702A (en) * | 2013-06-04 | 2014-12-17 | 吉林康乃尔药业有限公司 | Salvia miltiorrhiza snakegourd peel tablet fingerprint detection method |
| CN103760272A (en) * | 2014-01-27 | 2014-04-30 | 山东齐都药业有限公司 | Method for constructing feature fingerprint spectrum of rhizoma gastrodiae and ligusticum wallichii particle product |
| CN104237444A (en) * | 2014-10-15 | 2014-12-24 | 辽宁中医药大学 | Construction method of HPLC (High Performance Liquid Chromatography) fingerprint spectrum of salvia miltiorrhiza and radix puerariae depression relieving drug |
| CN105842353A (en) * | 2016-03-18 | 2016-08-10 | 江苏康缘药业股份有限公司 | Establishing method of fingerprint spectrum of honeysuckle-fructus forsythiae heat-clearing tablets and fingerprint spectrum |
| CN105758978A (en) * | 2016-04-25 | 2016-07-13 | 广西壮族自治区梧州食品药品检验所 | Method for measuring contents of various ingredients in cold cough syrup |
Non-Patent Citations (3)
| Title |
|---|
| 丁宁 等: "HPLC-DAD法测定冠脉宁片中葛根素和丹酚酸B的量及其指纹图谱研究", 《首都医科大学学报》 * |
| 王莎 等: "复方龙脉宁HPLC指纹图谱的研究", 《中华中医药学刊》 * |
| 董自亮 等: "华盖散制剂-药材谱峰匹配指纹图谱研究", 《中草药》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114778710A (en) * | 2022-02-21 | 2022-07-22 | 吉林修正药业新药开发有限公司 | HPLC (high Performance liquid chromatography) characteristic map construction method of forsythia suspense toxin-vanquishing tablets and method for monitoring quality of forsythia suspense toxin-vanquishing tablets |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106645459B (en) | 2018-10-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109406645B (en) | Detection method of ephedra, fried bitter apricot seed, liquorice and scutellaria baicalensis in children asthma-relieving oral liquid | |
| CN110031570B (en) | Fingerprint spectrum detection method of anti-cold granules | |
| CN105842353B (en) | The method for building up and its finger-print of Lonicera and Forsythia heat clearing tablet finger-print | |
| CN109633001B (en) | Fingerprint detection method of wine glossy privet fruit | |
| CN106645508A (en) | Construction method of HPLC specific chromatogram of wind-heat common cold granule | |
| CN110736799B (en) | Quality detection method of traditional Chinese medicine children's cold-relieving granules | |
| CN106932509B (en) | Radix astragali and radix puerariae particle fingerprint spectrum and construction method thereof | |
| CN106353430A (en) | Qianliexin capsule quality evaluation method based on multi-index active ingredient measurement | |
| CN105675739A (en) | Construction method of HPLC specific chromatogram of traditional Chinese medicines treating wind-heat cold | |
| CN107328872A (en) | The construction method and its standard finger-print of radix scrophulariae HPLC-FPS | |
| CN107271598A (en) | The construction method of Chinese patent drug Yanyan slice standard feature collection of illustrative plates and application | |
| CN104316613A (en) | Method for establishing fingerprint spectrum of wind-dispelling and detoxifying capsules | |
| CN104792889B (en) | The fingerprint of a kind of Yinhuang lung clearing preparation and standard finger-print thereof and application | |
| CN108254470A (en) | In glutinous rehmannia while carbohydrate content measure and its fingerprint map construction method | |
| CN110441413B (en) | Construction method and detection method of HPLC fingerprint of Qianbai rhinitis tablets | |
| CN110568108B (en) | Multi-component content determination method of Ganfule preparation | |
| CN108663440B (en) | Method for constructing UPLC fingerprint spectrum of callicarpa nudiflora medicinal material and standard fingerprint spectrum | |
| CN107643343B (en) | HPLC fingerprint spectrum determination method of Yunv Jian standard soup | |
| CN105699506A (en) | HPLC fingerprint chromatogram establishing method of Chinese patent medicine 'dibutyl particles' | |
| CN106645459A (en) | Method for constructing HPLC specific chromatogram of granules for treating common cold with wind-cold syndrome | |
| CN104655748B (en) | A kind of erigeron breviscapus granule finger-print and its method for building up and application | |
| CN110274970A (en) | The method for building up for melting poor finger-print and its application in Yixiesheng capsule Quality Control | |
| CN110297047B (en) | Establishment method of HPLC fingerprint of lysimachia capillipes hemsl medicinal material and fingerprint thereof | |
| CN103487528A (en) | HPLC fingerprint determination method of cough relieving Bulbus fritillariae cirrhosae and loquat dripping pills | |
| CN113640432A (en) | Quality evaluation method of loins strengthening and body building pills |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190620 Address after: 130000 1369 Shunda Road, Changchun, Jilin Co-patentee after: Jilin Changbaishan Pharmaceutical Group Co., Ltd. Patentee after: Jilin xiuzheng pharmaceutical new medicine development Co., Ltd. Address before: 130000 1369 Shunda Road, Changchun, Jilin Patentee before: Jilin xiuzheng pharmaceutical new medicine development Co., Ltd. |