CN106644625A - Three-axis test sample preparation device of low-strength microorganism calcareous sand and application method thereof - Google Patents
Three-axis test sample preparation device of low-strength microorganism calcareous sand and application method thereof Download PDFInfo
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- CN106644625A CN106644625A CN201610880204.8A CN201610880204A CN106644625A CN 106644625 A CN106644625 A CN 106644625A CN 201610880204 A CN201610880204 A CN 201610880204A CN 106644625 A CN106644625 A CN 106644625A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/286—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q involving mechanical work, e.g. chopping, disintegrating, compacting, homogenising
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Abstract
The invention discloses a three-axis test sample preparation device of low-strength microorganism calcareous sand and an application method thereof. A sample preparation device assembly comprises a mold barrel, a three-axis instrument base and a compaction device. The mold barrel comprises two pieces of splitting molds, a lower test sample cap and a rubber plug. Upper and lower ends of the mold barrel are matched with the rubber plug and the lower test sample cap respectively. The two pieces of splitting molds are hooped through a tightening ring sleeve. A rubber sealing strip is stuck on a vertical section of each splitting mold. Each splitting mold is provided with an exhausting hole. No squeezing disturbance on the test sample is caused by preparation, sampling and sample filling processes of microorganism soil, and reliable static and dynamic characteristic parameters of the low-strength microorganism calcareous sand can be obtained. The application method of the test sample preparation device comprises the steps of assembling the mold barrel, filling a sand soil sample, grouting and reinforcing microorganisms, mounting and removing the mold barrel on a three-axis instrument and the like.
Description
Technical field
The present invention relates to civil engineering experimental technique field, more particularly to the axle sample sample preparation of low-intensity microorganism calcareous sand three
Device and using method.
Background technology
Calcareous sand is a kind of Special Rock medium of the marine organisms origin cause of formation, and in China, South China Sea is widely distributed.Calcium
Matter sand only sees that the South Sea is civilian and defence engineering as subsoil major part.It is characterized in that foundation bearing capacity requirement is low, builds
Build that load is little, as China proposes the strategic objective of " building ocean power ", China will carry out energetically the exploitation on the South China Sea Islands with
Build, wherein harbour, airfield runway and when building of all kinds of civilian military architectures, inevitably run into calcareous sand ground and ask
Topic.Substantial amounts of test and Engineering Projects show that traditional pile foundation engineering experience can not be applied to calcareous sand, therefore to South Sea calcium
Matter sandy ground base need to take measures to improve the mechanical property of the soil body.Traditional method for processing foundation have it is many include mechanical densification,
Chemical grouting, change fill out process etc..Microorganism grouting reinforcement technique is not only kept away compared with traditional cement or chemical grouting technology
Exempt from the construction disturbance of traditional grouting method, the impact to ecological environment is little, and grouting pressure is little, transmission radius is big, solidification
Reaction time is short.Therefore, microorganism grouting reinforcement technique starts in ground work as a kind of new stiffening basic ground technology
Journey field is taken seriously.In prior art, the microorganism solidification equipment of sand is applied to reinforces shaping and the higher sample of intensity
The mechanical tests such as unconfined compression test, uniaxial compressive test are carried out, but for low-intensity reinforces sample, is had the disadvantages that：
(1) reinforcing shaping sample directly cannot be installed to sample on triaxial apparatus device, be unfavorable for reinforcing sand directly as microorganism
Quiet dynamic triaxial tests sample preparation device.(2) when preparing low-intensity sample using the device, can cause to reinforce because of disturbance during sample is torn open
Sample is destroyed, it is impossible to obtain complete low-intensity sample.(3) the device rubber membrane with fit not closely, in sample sample preparation into mould cylinder
During cannot meet very well intended diameter requirement, dress sample it is uneven.Therefore, to carry out for the cementing calcareous sand ground of microorganism
The dynamic and static properties development test of technology, it is necessary to which exploitation sets suitable for the slip casting instrument of low-intensity biology calcareous sand triaxial test
It is standby.
The content of the invention
It is an object of the invention to provide a kind of sample suitable for microbiological treatment low-intensity soil body force model triaxial test
Sample preparation device and its using method, solve the problems, such as that microorganism is reinforced malm and disturbance destruction easily occurs under low-intensity, protect
The integrality of card sample.
The technical scheme adopted to realize the object of the invention is a kind of such, the axle of low-intensity microorganism calcareous sand three examination
Sample sample preparation device, including mould cylinder, triaxial apparatus base and densification device.
The mould cylinder includes split cylinder, latex plug and lower sample cap.
The split cylinder can axially symmetricly be split as split cavity I and split cavity II.The split cavity I and split cavity II
Reality can tightly be closed.Cannelure I and cannelure II are provided with the split cylinder inner wall.The cannelure I is located on split cylinder
Portion.The cannelure II is located at split cylinder bottom.Through hole is provided with the split cylinder side wall.The split cylindrical outer set
There are at least two lock ring ring sets.The lock ring ring set clamps split cylinder.
The latex plug fills in the upper end of split cylinder.Pulp-inlet pipe is provided with the latex plug.The pulp-inlet pipe connection breast
The upper and lower surface of plug.
The lower sample cap is ladder revolving body, and the cylinder I and cylinder II being coaxially disposed is followed successively by from top to bottom.The circle
Cannelure III is provided with the side wall of post I.The upper surface of the cylinder I is embedded with permeable stone I.Grout outlet is additionally provided with the cylinder I.It is described
Grout outlet is connected outside the bottom of permeable stone I and the side wall of cylinder I.After the assembly of mould cylinder, cannelure II is concordant with the position of cannelure III,
And it is embedded with rubber ring between cannelure II and cannelure III.The cylinder I fills in the lower end of split cylinder.The cylinder II is inserted
Triaxial apparatus base, and be fixedly connected with triaxial apparatus base.
During dress sample, the densification device can move up and down in mould cylinder.
Further, rubber weather strip is posted on the Mosaic face of the split cavity I and split cavity II.
Further, the lock ring ring set is provided with some bolts hole.Bolt is installed in the bolt hole.Bolt will be split
Mould I and the banding of split cavity II and fixation.
Further, the split cylinder uses clear PVC material.The split cylinder is provided with scale.
Invention additionally discloses a kind of using method of the axle sample sample preparation device of low-intensity microorganism calcareous sand three, including it is following
Step：
1) rubber membrane rubber ring is fixed on lower sample cap.
2) split cavity I and split cavity II are placed in the outer surface of rubber membrane, and with lock ring ring set by split cavity I and split cavity
II securely fixes together, then lentamente routs up rubber membrane from split cylinder.
3) it is evacuated by through hole using vavuum pump, makes rubber membrane be close to mould cylinder inwall.
4) filter paper is laid in mould cylinder, layering fills sample, and using densification device densification sample, until it reaches predetermined altitude.
Filter paper is laid at the top of sample, permeable stone II is placed on filter paper.Latex plug is stoppered, stops pumping.By mould cylinder brandreth stand
It is good.
5) grout outlet is closed, 1-1.5 times of pore volumes is poured into from pulp-inlet pipe with the speed of 2ml/min by peristaltic pump
Microbial bacterial liquid, opens grout outlet and discharges bacterium solution after standing 2 hours.
6) grout outlet is again switched off, 1-1.3 times of pore-bodies is poured into from pulp-inlet pipe with the speed of 1ml/min by peristaltic pump
Long-pending plus solid-liquid, the opening grout outlet discharge first round adds solid-liquid after standing 12 hours.
7) repeat step 5) and 6) more than 3 times until reach predetermined reinforcement criteria.
8) deionized water of more than 10 times pore volumes is poured into from pulp-inlet pipe with the speed of 5ml/min by peristaltic pump, from
Remaining plus solid-liquid and microbial bacterial liquid inside grout outlet drain sample, will subsequently reinforce sample and is placed in baking together with mould cylinder
Case is dried 72 hours at 60 DEG C.
9) cylinder II is inserted into triaxial apparatus base, tightens bolt and lower sample cap is fixed on triaxial apparatus base.By pulp
Pipe is connected with drainpipe under triaxial apparatus.
10) latex plug is pulled out, sample cap stopper enters split cylinder upper end on triaxial apparatus, with rubber ring by rubber membrane extension
Tighten the sample cap on triaxial apparatus.
11) triaxial apparatus applies 10kPa negative pressure by grout outlet to sample inside.Lock ring ring set is removed, the He of split cavity I is removed
Split cavity II.
Further, step 5) described in microbial bacterial liquid be Sporosarcina pasteurii.The training of every liter of bacterium
Contain 20g yeast extracts, 10g ammonium chlorides, 10mg manganese sulfate monohydrates and the water nickel chlorides of 24mg six in foster base.Culture medium PH is
9.0.Step 6) described in plus solid-liquid be formulated by calcium chloride, urea, bacteria culture media and deionized water.Per liter adds in solid-liquid
Containing 0.5mol calcium chloride, 0.5mol urea and 100ml bacteria culture medias.
The solution have the advantages that mathematical：
A) mould cylinder ease of assembly and can separate split cavity using split cavity method for designing, it is to avoid microorganism reinforces sand
Process and sampling process are reinforced sample and are damaged to low-intensity, so as to ensure the integrality of sample structure；
B the lower sample cap) tested in sample preparation device matches with triaxial apparatus base, completes directly to pacify in mould cylinder after sample preparation
It is mounted on triaxial apparatus, it is to avoid disturbance of the dress sample process to sample, to obtain more reliable geotechnical centrifuge model data；
C the split cavity side for) testing mould cylinder in sample preparation device is provided with aspirating hole, and vertical face posts rubber weather strip, is used in combination
Lock ring banding split cavity, meets and make rubber membrane be close to mould inner wall by aspirating hole pumping during dress sample, it is ensured that three axle sample preparations
Meeting groove together be provided with lower sample cap inside normal diameter requirement, split cavity and place rubber ring, can effective control sample cap
The positioning of installation process, and can guarantee that mould cylinder is close to prevent occurring side spillage in reinforcing process with sample cap；
D) test mould cylinder in sample preparation device and adopt special transparent PVC split cavity with a scale, can pass through during dress sample split
Mould observation dress sample, according to relative compaction, with the use of densification device, control dress sample height, is flattened after the completion of dress sample with densification device
Specimen surface, the present invention can in effective control sample making course sample relative compaction and uniformity；
E) the method during reperfusion reinforcement liquid using saturation grouting in combination with reaction is stood, it is ensured that chemical reaction
Process fully, uniformly carries out inside sample, the method relatively before microorganism consolidation by grouting method improve plus solid-liquid
Utilization rate, while it is more preferable to make the sample produced reinforce uniformity.
Description of the drawings
Fig. 1 is sample preparation device structural representation；
Fig. 2 is lock ring ring set and split cylinder connection diagram；
Fig. 3 is split cavity structural representation；
Fig. 4 is lower sample cap schematic diagram；
Fig. 5 is densification device schematic diagram.
In figure：Split cylinder (1), split cavity I (101), split cavity II (102), cannelure I (103), cannelure II
(104), through hole (105), rubber weather strip (106), lower sample cap (2), cylinder I (201), cannelure III (2011), permeable stone I
(2012), grout outlet (2013), cylinder II (202), lock ring ring set (3), bolt hole (301), bolt (302), latex plug (4),
Pulp-inlet pipe (401), densification device (5), triaxial apparatus base (6), rubber membrane (7), rubber ring (8), filter paper (9), permeable stone II (10).
Specific embodiment
With reference to embodiment, the invention will be further described, but should not be construed above-mentioned subject area of the invention only
It is limited to following embodiments.Without departing from the idea case in the present invention described above, according to ordinary skill knowledge and used
With means, various replacements and change are made, all should be included within the scope of the present invention.
Embodiment 1：
A kind of axle sample sample preparation device of low-intensity microorganism calcareous sand three, including mould cylinder, triaxial apparatus base 6 and densification device
5。
Referring to Fig. 1, the mould cylinder includes split cylinder 1, latex plug 4 and lower sample cap 2.
The split cylinder 1 uses clear PVC material.The split cylinder 1 is provided with scale.The split cylinder 1 can
Split cavity I 101 and split cavity II 102 are split as axially symmetricly.The split cavity I 101 and split cavity II 102 can be closed tightly
It is real.Referring to Fig. 3, rubber weather strip 106 is posted on the Mosaic face of the split cavity I 101 and split cavity II 102.It is described split
Cannelure I 103 and cannelure II 104 are provided with the inwall of cylinder 1.The cannelure I 103 is located at the top of split cylinder 1.The ring
Shape groove II 104 is located at the bottom of split cylinder 1.Through hole 105 is provided with the side wall of the split cylinder 1.Referring to Fig. 2, the split circle
Two lock ring ring sets 3 are cased with outside cylinder 1.The lock ring ring set 3 clamps split cylinder 1.The lock ring ring set 3 is provided with some
Bolt hole 301.Bolt 302 is installed in the bolt hole 301.Bolt 302 by split cavity I 101 and the banding of split cavity II 102 simultaneously
It is fixed.
The latex plug 4 fills in the upper end of split cylinder 1.Pulp-inlet pipe 401 is provided with the latex plug 4.The pulp-inlet pipe
The upper and lower surface of 401 connection latex plugs 4.
Referring to Fig. 4, the lower sample cap 2 is ladder revolving body, and the He of cylinder I 201 being coaxially disposed is followed successively by from top to bottom
Cylinder II 202.Cannelure III 2011 is provided with the side wall of the cylinder I 201.The upper surface of the cylinder I 201 is embedded with permeable stone I
2012.Grout outlet 2013 is additionally provided with the cylinder I 201.The grout outlet 2013 connects the bottom of permeable stone I 2012 and cylinder I
Outside 201 side walls.After the assembly of mould cylinder, cannelure II 104 is concordant with the position of cannelure III 2011, and cannelure II 104 and ring
Rubber ring 8 is embedded between shape groove III 2011, to ensure that there is good air-tightness between split cylinder 1 and lower sample cap 2.It is described
Cylinder I 201 fills in the lower end of split cylinder 1.The insertion triaxial apparatus of the cylinder II 202 base 6, and fix with triaxial apparatus base 6
Connection.
During dress sample, the densification device 5 can move up and down in mould cylinder and densification is carried out to the calcareous sand in mould cylinder, control
The relative compaction of sample.
Embodiment 2：
The present embodiment discloses a kind of axle sample sample preparation device of low-intensity microorganism calcareous sand three with regard to described in embodiment 1
Using method, comprises the following steps：
1) rubber membrane 7 is fixed on lower sample cap 2 with rubber ring 8.
2) split cavity I 101 and split cavity II 102 are placed in the outer surface of rubber membrane, rubber ring 8 snaps in cannelure II 104,
And together with split cavity I 101 is securely fixed with split cavity II 102 with lock ring ring set 3, then lentamente by rubber membrane 7 from right
Open and routed up in cylinder 1.
3) it is evacuated by through hole 105 using vavuum pump, makes rubber membrane 7 be close to mould cylinder inwall.
4) filter paper 9 is laid in mould cylinder.The gross weight of sample is calculated according to relative compaction and volume, then sample
It is divided into several pieces.Using sand rain method layering dress sample, each layer of sample uses the densification sample of densification device 5, and Control Assay height is whole
Flat sample surface, it is ensured that the relative compaction of dress sample, until sample reaches predetermined altitude.Filter paper 9, filter paper are laid at the top of sample
Permeable stone II 10 is placed on 9.Latex plug 4 is stoppered, stops pumping.Mould cylinder is good with brandreth stand.
5) grout outlet 2013 is closed, 1-1.5 times of holes is poured into from pulp-inlet pipe 401 with the speed of 2ml/min by peristaltic pump
The microbial bacterial liquid of volume, opens grout outlet 2013 and discharges bacterium solution after standing 2 hours.
6) grout outlet 2013 is again switched off, 1-1.3 times is poured into from pulp-inlet pipe 401 with the speed of 1ml/min by peristaltic pump
Pore volume plus solid-liquid, stand 12 hours after open grout outlet 2013 discharge the first round add solid-liquid.
7) repeat step 5) and 6) more than 3 times until reach predetermined reinforcement criteria.
8) deionized water of more than 10 times pore volumes is poured into from pulp-inlet pipe 401 with the speed of 5ml/min by peristaltic pump,
Remaining plus solid-liquid and microbial bacterial liquid from inside the drain sample of grout outlet 2013, will subsequently reinforce sample together with mould cylinder
It is placed in baking oven to dry 72 hours at 60 DEG C.
9) by the insertion triaxial apparatus of cylinder II 202 base 6, tighten bolt and lower sample cap 2 is fixed on triaxial apparatus base 6.
Grout outlet 2013 is connected with drainpipe under triaxial apparatus.
10) latex plug 4 is pulled out, sample cap stopper enters the upper end of split cylinder 1 on triaxial apparatus, is stretched out rubber membrane 7 with rubber ring 8
Part tightens the sample cap on triaxial apparatus.
11) triaxial apparatus applies 10kPa negative pressure by grout outlet 2013 to sample inside.Whole sample is prepared and completed.Remove
Lock ring ring set 3, removes split cavity I 101 and split cavity II 102.Now sample has been saturated sample, and undisturbed.
12) sample is put into the balancing gate pit of sealing, is carried out applying confined pressure, is applied the series of steps such as axial compressive force, until
Sample sheared destruction.Triaxial test is carried out to multiple samples, sample shearing strength envelope curve is drawn.
What deserves to be explained is, step 5 in the present embodiment) described in microbial bacterial liquid be Sporosarcina
pasteurii.Contain 20g yeast extracts, 10g ammonium chlorides, 10mg manganese sulfate monohydrates and 24mg in the culture medium of every liter of bacterium
Six water nickel chlorides.Culture medium PH is 9.0.Step 6) described in plus solid-liquid by calcium chloride, urea, bacteria culture media and deionized water
It is formulated.Per liter adds in solid-liquid containing 0.5mol calcium chloride, 0.5mol urea and 100ml bacteria culture medias.
Claims (6)
1. the axle sample sample preparation device of a kind of low-intensity microorganism calcareous sand three, it is characterised in that including mould cylinder, triaxial apparatus base
And densification device (5) (6)；
The mould cylinder includes split cylinder (1), latex plug (4) and lower sample cap (2)；
The split cylinder (1) can axially symmetricly be split as split cavity I (101) and split cavity II (102)；The split cavity I
And split cavity II (102) can tightly close reality (101)；Cannelure I (103) and cannelure are provided with split cylinder (1) inwall
Ⅱ(104)；The cannelure I (103) is positioned at split cylinder (1) top；The cannelure II (104) is positioned at split cylinder (1)
Bottom；Through hole (105) is provided with the wall of split cylinder (1) side；At least two lock ring rings are cased with outside the split cylinder (1)
Set (3)；The lock ring ring set (3) clamps split cylinder (1)；
The latex plug (4) fills in the upper end of split cylinder (1)；Pulp-inlet pipe (401) is provided with the latex plug (4)；It is described enter
The upper and lower surface of slurry pipe (401) connection latex plug (4)；
The lower sample cap (2) is ladder revolving body, and the cylinder I (201) and cylinder II being coaxially disposed is followed successively by from top to bottom
(202)；Cannelure III (2011) is provided with the side wall of the cylinder I (201)；The upper surface of the cylinder I (201) is embedded with permeable stone I
(2012)；Grout outlet (2013) is additionally provided with the cylinder I (201).Grout outlet (2013) connection permeable stone I (2012) bottom
Outside portion and the side wall of cylinder I (201)；After the assembly of mould cylinder, cannelure II (104) is concordant with the position of cannelure III (2011), and
The rubber ring (8) is embedded between cannelure II (104) and cannelure III (2011)；The cylinder I (201) fills in split cylinder
(1) lower end；Insertion triaxial apparatus base of the cylinder II (202) (6), and be fixedly connected with triaxial apparatus base (6)；
During dress sample, the densification device (5) can move up and down in mould cylinder.
2. the axle sample sample preparation device of a kind of low-intensity microorganism calcareous sand three according to claim 1, it is characterised in that：Institute
Rubber weather strip (106) is posted on the Mosaic face for stating split cavity I (101) and split cavity II (102).
3. a kind of axle sample sample preparation device of low-intensity microorganism calcareous sand three according to claim 1 and 2, its feature exists
In：The lock ring ring set (3) is provided with some bolts hole (301)；Bolt (302) is installed in the bolt hole (301)；Bolt
(302) by split cavity I (101) and the banding of split cavity II (102) and fixation.
4. a kind of axle sample sample preparation device of low-intensity microorganism calcareous sand three according to claim 1,2 or 3, its feature exists
In：The split cylinder (1) uses clear PVC material；The split cylinder (1) is provided with scale.
5. a kind of using method of the axle sample sample preparation device of low-intensity microorganism calcareous sand three with regard to described in claim 1, its
It is characterised by：Comprise the following steps：
1) rubber membrane (7) is fixed on lower sample cap (2) with rubber ring (8)；
2) split cavity I (101) and split cavity II (102) are placed in the outer surface of rubber membrane, and with lock ring ring set (3) by split cavity
I (101) and split cavity II (102) securely fix together, then lentamente rubber membrane (7) routed up from split cylinder (1)；
3) it is evacuated by through hole (105) using vavuum pump, makes rubber membrane (7) be close to mould cylinder inwall；
4) filter paper (9) is laid in mould cylinder, layering fills sample, and using densification device (5) densification sample, until it reaches predetermined height
Degree；Filter paper (9) is laid at the top of sample, permeable stone II (10) is placed on filter paper (9)；Latex plug (4) is stoppered, stops pumping；Will
Mould cylinder is good with brandreth stand；
5) grout outlet (2013) is closed, 1-1.5 times of holes is poured into from pulp-inlet pipe (401) with the speed of 2ml/min by peristaltic pump
The microbial bacterial liquid of volume, opens grout outlet (2013) and discharges bacterium solution after standing 2 hours；
6) grout outlet (2013) is again switched off, 1-1.3 times is poured into from pulp-inlet pipe (401) with the speed of 1ml/min by peristaltic pump
Pore volume plus solid-liquid, stand 12 hours after open grout outlet (2013) discharge the first round add solid-liquid；
7) repeat step 5) and 6) more than 3 times until reach predetermined reinforcement criteria；
8) deionized water of more than 10 times pore volumes is poured into from pulp-inlet pipe (401) with the speed of 5ml/min by peristaltic pump, from
Remaining plus solid-liquid and microbial bacterial liquid inside grout outlet (2013) drain sample, will subsequently reinforce sample together with mould cylinder
It is placed in baking oven to dry 72 hours at 60 DEG C；
9) by insertion triaxial apparatus base of cylinder II (202) (6), tighten bolt and lower sample cap (2) is fixed on into triaxial apparatus base (6)
On；Grout outlet (2013) is connected with drainpipe under triaxial apparatus；
10) latex plug (4) is pulled out, sample cap stopper enters split cylinder (1) upper end on triaxial apparatus, with rubber ring (8) by rubber membrane (7)
Extension tightens the sample cap on triaxial apparatus；
11) triaxial apparatus applies 10kPa negative pressure by grout outlet (2013) to sample inside；Lock ring ring set (3) is removed, is removed split
Mould I (101) and split cavity II (102).
6. a kind of axle sample system of low-intensity microorganism calcareous sand three with regard to described in claim 1 according to claim 5
The using method of sampling device, it is characterised in that：Step 5) described in microbial bacterial liquid be Sporosarcina pasteurii；
Contain 20g yeast extracts, 10g ammonium chlorides, 10mg manganese sulfate monohydrates and the water nickel chlorides of 24mg six in the culture medium of every liter of bacterium；
Culture medium PH is 9.0；Step 6) described in plus solid-liquid be formulated by calcium chloride, urea, bacteria culture media and deionized water；Often
Rise to add and contain in solid-liquid 0.5mol calcium chloride, 0.5mol urea and 100ml bacteria culture medias.
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| CN201610880204.8A CN106644625B (en) | 2016-10-09 | 2016-10-09 | A kind of test method based on three axis sample sample preparation device of low-intensity microorganism calcareous sand |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202126369U (en) * | 2011-06-28 | 2012-01-25 | 宁波大学 | Sample preparation instrument of sand triaxial test |
| CN103048174A (en) * | 2012-12-21 | 2013-04-17 | 大连理工大学 | Triaxial sample preparation device for low-liquid limit silty soil and sample preparation method |
| CN105115794A (en) * | 2015-07-02 | 2015-12-02 | 河海大学 | Sample production apparatus and sample production method of layered rock-soil body sample |
| CN204988817U (en) * | 2015-04-14 | 2016-01-20 | 山西省交通科学研究院 | Simple and easy triaxial soil sample humidifying device |
| CN105865874A (en) * | 2016-05-11 | 2016-08-17 | 同济大学 | Sample preparing device suitable for sandy soil laboratory tests |
| CN205749066U (en) * | 2016-07-04 | 2016-11-30 | 中国科学院武汉岩土力学研究所 | A kind of preparation facilities of geotechnological three axle sand samples |
-
2016
- 2016-10-09 CN CN201610880204.8A patent/CN106644625B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202126369U (en) * | 2011-06-28 | 2012-01-25 | 宁波大学 | Sample preparation instrument of sand triaxial test |
| CN103048174A (en) * | 2012-12-21 | 2013-04-17 | 大连理工大学 | Triaxial sample preparation device for low-liquid limit silty soil and sample preparation method |
| CN204988817U (en) * | 2015-04-14 | 2016-01-20 | 山西省交通科学研究院 | Simple and easy triaxial soil sample humidifying device |
| CN105115794A (en) * | 2015-07-02 | 2015-12-02 | 河海大学 | Sample production apparatus and sample production method of layered rock-soil body sample |
| CN105865874A (en) * | 2016-05-11 | 2016-08-17 | 同济大学 | Sample preparing device suitable for sandy soil laboratory tests |
| CN205749066U (en) * | 2016-07-04 | 2016-11-30 | 中国科学院武汉岩土力学研究所 | A kind of preparation facilities of geotechnological three axle sand samples |
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