CN106638074A - Pre-treatment process of lignocellulose raw material - Google Patents

Pre-treatment process of lignocellulose raw material Download PDF

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Publication number
CN106638074A
CN106638074A CN201611219083.9A CN201611219083A CN106638074A CN 106638074 A CN106638074 A CN 106638074A CN 201611219083 A CN201611219083 A CN 201611219083A CN 106638074 A CN106638074 A CN 106638074A
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China
Prior art keywords
raw material
steam explosion
treatment process
pretreating process
loss
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Pending
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CN201611219083.9A
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Chinese (zh)
Inventor
张宁
魏拥辉
李春玲
陈伟光
闫伟松
吴杨
彭霞
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COFCO BIOCHEMICAL ENERGY (ZHAODONG) Co Ltd
Cofco Corp
Cofco Nutrition and Health Research Institute Co Ltd
Original Assignee
COFCO BIOCHEMICAL ENERGY (ZHAODONG) Co Ltd
Cofco Corp
Cofco Nutrition and Health Research Institute Co Ltd
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Priority to CN201611219083.9A priority Critical patent/CN106638074A/en
Publication of CN106638074A publication Critical patent/CN106638074A/en
Pending legal-status Critical Current

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    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21B—FIBROUS RAW MATERIALS OR THEIR MECHANICAL TREATMENT
    • D21B1/00—Fibrous raw materials or their mechanical treatment
    • D21B1/02—Pretreatment of the raw materials by chemical or physical means
    • D21B1/021—Pretreatment of the raw materials by chemical or physical means by chemical means
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
    • C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
    • C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
    • C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • D—TEXTILES; PAPER
    • D21—PAPER-MAKING; PRODUCTION OF CELLULOSE
    • D21B—FIBROUS RAW MATERIALS OR THEIR MECHANICAL TREATMENT
    • D21B1/00—Fibrous raw materials or their mechanical treatment
    • D21B1/04—Fibrous raw materials or their mechanical treatment by dividing raw materials into small particles, e.g. fibres
    • D21B1/12—Fibrous raw materials or their mechanical treatment by dividing raw materials into small particles, e.g. fibres by wet methods, by the use of steam
    • D21B1/30—Defibrating by other means
    • D21B1/36—Explosive disintegration by sudden pressure reduction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2201/00—Pretreatment of cellulosic or lignocellulosic material for subsequent enzymatic treatment or hydrolysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P2203/00—Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Wood Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Chemical & Material Sciences (AREA)
  • Mechanical Engineering (AREA)
  • Zoology (AREA)
  • Polymers & Plastics (AREA)
  • Medicinal Chemistry (AREA)
  • Materials Engineering (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Sustainable Development (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)

Abstract

The invention relates to the technical field of cellulosic ethanol and discloses a pre-treatment process of a lignocellulose raw material. The pre-treatment process disclosed by the invention performs steam explosion pre-treatment on the lignocellulose raw material at 155-160 DEG C at an acid concentration of 2.5-3.0%. By adjusting the steam explosion temperature, acid concentration and cooking time in proper range values at the same time, a purpose of reducing the loss of the steam explosion pre-treatment process and the unit consumption of an ethanol raw material per ton is achieved based on reduction of loss of cellulose and hemicelluloses. The obtained steam explosion material can fully transfer the ability of a C5/C6 co-fermented strain, so that the production efficiency of cellulosic ethanol is improved.

Description

A kind of pretreating process of lignocellulosic material
Technical field
The present invention relates to cellulosic ethanol technical field, particularly relates to a kind of pretreatment of lignocellulosic material Technique.
Background technology
The energy and environmental crisis that 21 century is faced, it has also become the major obstacle of Development of Human Civilization.In the face of increasingly short The scarce energy, people are look for the new forms of energy of substitute fossil fuels.Ethanol is bio liquid energy substance, is also fossil combustion Expect most probable substitute.
Ethanol can be synthesized by oil, or can be obtained by microorganism conversion biomass.The ethanol of the global overwhelming majority is just It is to be obtained by the method for biofermentation.The raw material of Ethanol Production by Fermentation has three kinds：Starch, carbohydrate and cellulose family.Mesh Before, it is raw material (such as various crop materials) using lignocellulosic, taking the method for biofermentation, to obtain ethanol be a kind of non- Normal promising method.
Because the composition component of lignocellulosic is complicated and stable, there is many physics and chemistry barrier, make enzyme system Agent is difficult to be contacted with cellulose, it is impossible to carry out enzymatic reaction rapidly.Therefore, plant cellulose material has to pass through before enzyme hydrolysis Pretreatment.In numerous pretreating process, steam explosion technology is the pretreating process being most widely used.
The bacterial strain for being presently used for cellulosic ethanol production mostly is offshore company's production, the C5/C6 common fermentation bacterial strains of such as Pu Du Believe NS22202 common fermentation bacterial strains with Novi, both are compared in glucose utilization rate quite, but the latter is in the utilization rate of wood sugar On but exceed well over the former.But, be present technical defect in current steam blasting pretreating process, make more than 50% half fine Dimension element in steam blasting preprocessing process lose, so just cannot give full play to as NS22202 is this send out altogether fermentability compared with The ability of good bacterial strain, while also increasing the loss in steam blasting pretreatment.
The content of the invention
In view of this, it is an object of the invention to provide a kind of pretreating process of lignocellulosic material so that described Pretreating process reduce lignocellulosic material in cellulose and hemicellulose loss on the basis of, while reduce steam explosion locate in advance The loss of science and engineering skill and ton ethanol raw material unit consumption.
To achieve these goals, the present invention provides following technical scheme：
A kind of pretreating process of lignocellulosic material, 155-160 DEG C, 2.5-3.0% acid concentrations, digestion time Steam blasting pretreatment is carried out to lignocellulosic material under 20min.
For existing steam blasting preconditioning technique easily hemicellulose substantial amounts of at any time, present invention discover that from steam explosion temperature, Acid concentration and digestion time are adjusted, and allow both while obtaining higher hemicellulose and cellulose in suitable scope The rate of recovery, reduces the loss of gas-explosive material.
The present invention specific embodiment in, the pretreating process can 155 DEG C, 3.0% acid concentration, digestion time Steam blasting pretreatment is carried out to lignocellulosic material under 20min；Or 160 DEG C, 2.5% acid concentration, digestion time 20min Under steam blasting pretreatment is carried out to lignocellulosic material.
Preferably, the lignocellulosic material is maize straw；The acid is sulfuric acid.
The present invention set up multiple combinations and to maize straw for the temperature and acid concentration of steam blasting pretreating process Steam explosion is carried out, is as a result shown, can be with higher than 60% only under temperature, acid concentration, the digestion time that the present invention is limited Hemicellulose to greatest extent the rate of recovery realize reduce steam explosion loss purpose.
From above technical scheme, present invention adjustment steam explosion temperature, acid concentration and digestion time are simultaneously in suitable Value range in, realize on the basis of the loss for reducing cellulose and hemicellulose, while reducing steam explosion pretreating process Loss and the purpose of ton ethanol raw material unit consumption, gained gas-explosive material can give full play to the ability of C5/C6 common fermentation bacterial strains, improve The production efficiency of cellulosic ethanol.
Specific embodiment
The embodiment of the invention discloses a kind of pretreating process of lignocellulosic material.Those skilled in the art can borrow Mirror present disclosure, is suitably modified technological parameter realization.Specifically, all similar replacements and change are to this area It is it will be apparent that they are considered as being included in the present invention for technical staff.The pretreating process of the present invention has passed through Preferred embodiment is described, and related personnel substantially can be in without departing from present invention, spirit and scope to described herein Pretreating process be modified or suitably change with combining realizing and apply the technology of the present invention.
For a further understanding of the present invention, the present invention is provided a kind of lignocellulosic material with reference to embodiment Pretreating process is described in detail.
Embodiment 1：Pretreating process of the present invention
Steam blasting is carried out under 155 DEG C, 3.0% sulfuric acid concentration, digestion time 20min to lignocellulosic material to locate in advance Reason.
Embodiment 2：Pretreating process of the present invention
Steam blasting is carried out under 160 DEG C, 2.5% sulfuric acid concentration, digestion time 20min to lignocellulosic material to locate in advance Reason.
Embodiment 3：The pretreating process contrast of different temperatures and sulfuric acid concentration
Different steam explosion temperature, sulfuric acid concentration and digestion time are set according to table 1, other steam explosions are consistent, then count Cellulose and the hemicellulose rate of recovery, steam loss, sulfuric acid loss after steam explosion, while carrying out cellulosic ethanol with gas-explosive material Production (Novi believes NS22202 common fermentation bacterial strains), counts ton ethanol corn stalk unit consumption, the results are shown in Table 2.
The different pretreatments technological parameter of table 1
Temperature Acid is dense Digestion time
Experimental example 1 160℃ 2.50% 20min
Experimental example 2 155℃ 3.00% 20min
Control 1 165℃ 3.00% 20min
Control 2 160℃ 2.00% 20min
Control 3 155℃ 3.50% 20min
Control 4 180℃ 3.00% 10min
Control 5 140℃ 2.50% 30min
Control 6 130℃ 1.50% 40min
Control 7 125℃ 1.00% 20min
Table 2
Control The cellulose rate of recovery The hemicellulose rate of recovery Steam mono-consumption Sulfuric acid unit consumption Ton ethanol unit consumption
Experimental example 1 88.59% 60.33% 9.67 0.17 6.7t
Experimental example 2 89.91% 65.55% 9.51 0.18 6.8t
Control 1 84.51% 44.65% 10.74 0.20 7.5t
Control 2 89.10% 59.62% 10.82 0.21 7.4t
Control 3 88.31% 58.38% 10.01 0.25 7.4t
Control 4 80.21% 33.86% 12.30 0.26 8.5t
Control 5 93.21% 70.23% 16.60 0.45 9.4t
Control 6 95.07% 78.52% 20.10 0.69 14.3t
Control 7 95.71% 85.31% 25.03 0.93 20.1t
By Tables 1 and 2 as can be seen that compareing 1 to control 4 not only in the hemicellulose rate of recovery than relatively low, while steam explosion Loss is also higher；Although and compare 5 to control 7 with the higher hemicellulose rate of recovery, due to pretreating effect it is poor, reality Border raw material availability is low, and steam explosion loss aspect is highest.Just have in the parameters that only present invention is limited and exceed Loss in terms of the 60% hemicellulose rate of recovery, and minimum steam explosion technique material.
The explanation of above example is only intended to help and understands the method for the present invention and its core concept.It should be pointed out that right For those skilled in the art, under the premise without departing from the principles of the invention, the present invention can also be carried out Some improvement and modification, these are improved and modification is also fallen in the protection domain of the claims in the present invention.

Claims (5)

1. a kind of pretreating process of lignocellulosic material, it is characterised in that 155-160 DEG C, 2.5-3.0% acid concentrations, Steam blasting pretreatment is carried out to lignocellulosic material under digestion time 20min.
2. pretreating process according to claim 1, it is characterised in that 155 DEG C, 3.0% acid concentration, digestion time Steam blasting pretreatment is carried out to lignocellulosic material under 20min.
3. pretreating process according to claim 1, it is characterised in that 160 DEG C, 2.5% acid concentration, digestion time Steam blasting pretreatment is carried out to lignocellulosic material under 20min.
4. the pretreating process according to claim 1-3 any one, it is characterised in that the lignocellulosic material is jade Rice stalk.
5. the pretreating process according to claim 1-3 any one, it is characterised in that the acid is sulfuric acid.
CN201611219083.9A 2016-12-26 2016-12-26 Pre-treatment process of lignocellulose raw material Pending CN106638074A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080057555A1 (en) * 2006-09-05 2008-03-06 Xuan Nghinh Nguyen Integrated process for separation of lignocellulosic components to fermentable sugars for production of ethanol and chemicals
CN103627735A (en) * 2013-11-28 2014-03-12 南京林业大学 Method for co-producing plurality of saccharic acids from cellulose fuel ethanol
CN105368881A (en) * 2015-12-10 2016-03-02 辽宁石油化工大学 Method for preparing fuel ethanol by joint fermentation of synanthrin containing material and cellulosic material
CN105624208A (en) * 2016-03-30 2016-06-01 辽宁石油化工大学 Method for producing ethanol by high-temperature enzymolysis and fermentation of lignocellulose
CN105780565A (en) * 2015-12-10 2016-07-20 辽宁石油化工大学 Lignocellulose raw material dilute acid steam explosion pretreatment method

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080057555A1 (en) * 2006-09-05 2008-03-06 Xuan Nghinh Nguyen Integrated process for separation of lignocellulosic components to fermentable sugars for production of ethanol and chemicals
CN103627735A (en) * 2013-11-28 2014-03-12 南京林业大学 Method for co-producing plurality of saccharic acids from cellulose fuel ethanol
CN105368881A (en) * 2015-12-10 2016-03-02 辽宁石油化工大学 Method for preparing fuel ethanol by joint fermentation of synanthrin containing material and cellulosic material
CN105780565A (en) * 2015-12-10 2016-07-20 辽宁石油化工大学 Lignocellulose raw material dilute acid steam explosion pretreatment method
CN105624208A (en) * 2016-03-30 2016-06-01 辽宁石油化工大学 Method for producing ethanol by high-temperature enzymolysis and fermentation of lignocellulose

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
张晓阳: "《纤维素生物质水解与应用》", 31 December 2012, 郑州大学出版社 *

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Application publication date: 20170510