CN106635589A - Preparation method of health-care abalone wine - Google Patents
Preparation method of health-care abalone wine Download PDFInfo
- Publication number
- CN106635589A CN106635589A CN201611082527.9A CN201611082527A CN106635589A CN 106635589 A CN106635589 A CN 106635589A CN 201611082527 A CN201611082527 A CN 201611082527A CN 106635589 A CN106635589 A CN 106635589A
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- Prior art keywords
- abalone
- wine
- internal organ
- abalone internal
- raw material
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12G1/00—Preparation of wine or sparkling wine
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Abstract
The invention discloses a preparation method of health-care abalone wine, aiming at solving the problems that the existing utilization rate of abalone internal organs is low and the nutrient component of the wine is single. The preparation method comprises the following steps: adding water into the abalone internal organs, and crushing and pulping; adding compound proteinase and carrying out enzymolysis; after deactivating enzyme, centrifuging, taking supernatant and concentrating; carrying out Sephadex G-100 gel chromatography on concentrated liquid to obtain abalone internal organ polysaccharide; selecting grapes and preparing brewing raw materials according to a convectional process; adding the abalone internal organ polysaccharide into the brewing raw materials; boiling and cooling; then adding wheat koji and saccharifying enzyme and saccharifying; after cooling, adding an OAU-1 strain and fermenting; squeezing fermented liquid; filtering and clarifying the fermented liquid; and boiling wine, storing and blending to prepare the health-care abalone wine. The preparation method disclosed by the invention is reasonable in process and the operation is feasible. The prepared health-care abalone wine has a good taste and contains rich functional components of the abalone internal organ polysaccharide, so that the immunity of human bodies can be enhanced.
Description
Technical field
The present invention relates to the preparation of alcoholic beverage, especially a kind of health care abalone preparation of wine.
Background technology
With the improvement of people's living standards and the enhancing of health care consciousness, because grape wine has several amino acids, mineral
Matter and vitamins and other nutritious components, can directly be absorbed by the body, and the physiological function to maintaining and adjusting human body plays good work
With, therefore grape wine obtains liking and popularizing rapidly for masses.But popular grape wine mostly is common glucose on existing market
Wine, does not add nutrition other compositions, its limited efficacy.
Abalone is referred to as " hat of seafood delights ", is listed in first of eight delicacies, and it is not only nutritious, and abalone has clearly
The effects such as lung, beneficial stomach, nourshing kidney qi-restoratives.A large amount of abalone internal organ are generated in abalone deep processing, because not suitable purposes, greatly
The abalone internal organ of amount can only be dropped or be processed into low value feed, cause the spirited wasting of resources and environmental pollution.And abalone internal organ
The 20～30% of abalone gross weight are accounted for, containing abundant polysaccharide.Jing researchs show that abalone internal organ polysaccharide can strengthen lymphocyte increasing
Grow, the killing ability of Peritoneal Macrophage Phagocytosis and NK cells, body cell immunocompetence is lifted, with anti-oxidant, anti-
Tumor function.How effectively utilizes abalone internal organ become numerous technical staff's problem demanding prompt solution.
The content of the invention
Abalone internal organ utilization rate is low in order to overcome the shortcomings of prior art, grape wine nutrient content is single, and the present invention is carried
For a kind of rational technology, operate feasible health care abalone preparation of wine.
The technical solution adopted for the present invention to solve the technical problems is：A kind of health care abalone preparation of wine,
It is characterized in that：It is through following steps：
（1）The pretreatment of raw material
The abalone internal organ for choosing fresh free from extraneous odour are raw material, in sending into disintegrating machine, add the water of 3～10 times of abalone viscera weights,
Broken, homogenate obtains abalone internal organ slurries；
（2）Enzymolysis
By step（1）In the abalone internal organ slurries that obtain add the compound protease of abalone viscera weights 0.5～5%, control temperature
30～50 DEG C, 4～20h of enzymolysis time obtains abalone internal organ enzymolysis liquid；
（3）Go out enzyme
By step（2）In the abalone internal organ enzymolysis liquid that obtains be heated to 90～95 DEG C, holding is gone out 10～30min of enzyme, and must go out enzyme
Abalone internal organ enzymolysis liquid；
（4）Concentration
By step（3）In the abalone internal organ enzymolysis liquid of the enzyme that goes out that obtains naturally cool to room temperature, be re-fed into centrifuge process,
Take supernatant；The supernatant is sent in vacuum concentration equipment, be concentrated into original volume 1/6～1/8 abalone internal organ enzymolysis is dense
Contracting liquid；
（5）Chromatography
By step（4）In the abalone internal organ enzymolysis concentrate that obtains using Sephadex G-100 gel chromatographies, obtain abalone internal organ
Polysaccharide；
（6）Abalone brewing grape wine
It is raw material to choose new fresh grape, and according to conventional preparation technology wine production raw material is made；In wine production raw material
Middle addition step（5）Obtained abalone internal organ polysaccharide, boils, and is cooled to 28～38 DEG C, adds wheat koji and carbohydrase to be saccharified；
20～30 DEG C are cooled to, are inoculated with into OAU-1 bacterial strains, sent in the fermentation tank that sky disappears and fermented；Fermentation ends are squeezed, system
Obtain mash；Mash is carried out into filtration clarification, then Jing decocts wine, storage is blent, and health care of the alcoholic strength in 8～20% (v/v) is obtained
Abalone grape wine.
Described compound protease is the one kind in bromelain, pepsin, neutral proteinase and flavor protease
More than.
Described wine production raw material is 2～5 with the weight ratio of abalone internal organ polysaccharide:1；The addition of described wheat koji
For wine production raw material and the 3.5～4% of many sugar weights of abalone internal organ；The addition of described carbohydrase is 80～300 single
Position/gram wine production raw material；The inoculum concentration of described OAU-1 bacterial strains is wine production raw material and many sugar weights of abalone internal organ
0.1～0.5%.
The present invention is that abalone internal organ add water, and is crushed, and adds protease to be digested after slurrying, and centrifuging and taking is thereon after the enzyme that goes out
Clear liquid is concentrated, and concentrate adopts Sephadex G-100 gel chromatographies, obtains abalone internal organ polysaccharide；Grape is chosen according to routine
Technique makes wine production raw material；Abalone internal organ polysaccharide is added in wine production raw material, is boiled, wheat koji is added after cooling
It is saccharified with carbohydrase, adds OAU-1 bacterial strains to be fermented after cooling；Squeeze mash；Mash filtrations are clarified, then Jing is decocted
Wine, storage, blends, and health care abalone grape wine is obtained.It is digested to abalone internal organ using compound protease, is improving function
While peptide yield, local flavor optimization is carried out to hydrolysate, remove bitter taste, improve mouthfeel.By the abalone of the com-parison and analysis present invention
The free amino acid of grape wine and check wine, polyphenol, mineral element and antioxidation activity, such as remove the energy of DPPH free radicals
Power, reducing power, the abalone grape wine of the present invention is higher than the nutritive value of check wine, and oxidation resistance is higher.Preparing
During, the OAU-1 bacterial strains that it is adopted are the novel fermentation bacterial strains of screening, can obtain the zymotic fluid of high enzyme vigor.This
It is bright that modern biotechnology is applied in the preparation of heath-function abalone grape wine, take full advantage of abalone internal organ many
Sugar so as to which functional components are transferred to greatest extent in grape wine, and in brewing grape wine flow process, do not change its production skill
Art, improves the trophism of traditional wine, is suitable to wider array of crowd and drinks, and strengthens body immunity.The preparation method of the present invention
Rational technology, operation is feasible, easily accomplishes scale production.
Specific embodiment
With reference to embodiment to being originally described further.
Embodiment 1
A kind of health care abalone preparation of wine, it is through following steps：
（1）The pretreatment of raw material
The abalone internal organ for choosing fresh free from extraneous odour are raw material, are sent into after cleaning up in disintegrating machine, add 6 times of abalone viscera weights
Water, crush, homogenate, obtain abalone internal organ slurries；
（2）Enzymolysis
By step（1）In the abalone internal organ slurries that obtain add answering for pepsin, neutral proteinase and flavor protease composition
Hop protein enzyme, wherein, pepsic addition is the 0.5% of abalone viscera weights, and the addition of neutral proteinase is 2%, wind
The addition of taste protease is 3%, controls temperature 45 C, and enzymolysis time 12h obtains abalone internal organ enzymolysis liquid；
（3）Go out enzyme
By step（2）In the abalone internal organ enzymolysis liquid that obtains be heated to 93 DEG C, holding is gone out enzyme 20min, the abalone internal organ of the enzyme that must go out
Enzymolysis liquid；
（4）Concentration
By step（3）In the abalone internal organ enzymolysis liquid of the enzyme that goes out that obtains naturally cool to room temperature, be re-fed into centrifuge process,
Take supernatant；The supernatant is sent in vacuum decker, is concentrated into the 1/7 of original volume and is obtained abalone internal organ enzymolysis concentrate；
（5）Chromatography
By step（4）In obtain abalone internal organ enzymolysis concentrate using GE companies Sephadex G-100 gel chromatographies, obtain
Abalone internal organ polysaccharide；
（6）Abalone brewing grape wine
It is raw material to choose new fresh grape, and according to conventional preparation technology wine production raw material is made；In wine production raw material
In by weight be 3:1 adds step（5）Obtained abalone internal organ polysaccharide, boils, and is cooled to 35 DEG C, adds wine production former
Wheat koji of the material with the 3.8% of many sugar weights of abalone internal organ, and the carbohydrase of 200 units is added by every gram of wine production raw material, enter
Row saccharification, is cooled to 25 DEG C, is inoculated with into OAU-1 bacterial strains, and the inoculum concentration of OAU-1 bacterial strains is wine production raw material and abalone internal organ
The 0.3% of many sugar weights, is then fed into being fermented in the fermentation tank that sky disappears；Fermentation ends are squeezed, and mash is obtained；By wine with dregs
Liquid carries out filtration clarification, and then Jing decocts wine, and storage is blent, and health care abalone grape wine of the alcoholic strength in 12% (v/v) is obtained.
A kind of health care abalone preparation of wine that the present embodiment is provided, its rational technology operates feasible, Yi Shi
Existing large-scale production.In preparation process, abalone internal organ are digested using compound protease, improving the same of function peptide yield
When, and local flavor optimization is carried out to hydrolysate, and bitter taste is removed, improve mouthfeel.The OAU-1 bacterial strains that it is adopted are the new of screening
Fermentation strain, can obtain the zymotic fluid of high enzyme vigor.The functional components of abalone internal organ polysaccharide are transferred to grape by the present invention
In wine, and do not change in brewing grape wine flow process, the perfect trophism of grape wine can strengthen body immunity.
Embodiment 2
A kind of health care abalone preparation of wine, it is through following steps：
（1）The pretreatment of raw material
The abalone internal organ for choosing fresh free from extraneous odour are raw material, are sent into after cleaning up in disintegrating machine, add abalone viscera weights 10
Water again, crushes, and homogenate obtains abalone internal organ slurries；
（2）Enzymolysis
By step（1）In the abalone internal organ slurries that obtain add answering for bromelain, pepsin and flavor protease composition
Hop protein enzyme, wherein, the addition of bromelain is the 1% of abalone viscera weights, and pepsic addition is 2%, local flavor
The addition of protease is 2%, controls temperature 50 C, and enzymolysis time 4h obtains abalone internal organ enzymolysis liquid；
（3）Go out enzyme
By step（2）In the abalone internal organ enzymolysis liquid that obtains be heated to 95 DEG C, holding is gone out enzyme 10min, the abalone internal organ of the enzyme that must go out
Enzymolysis liquid；
（4）Concentration
By step（3）In the abalone internal organ enzymolysis liquid of the enzyme that goes out that obtains naturally cool to room temperature, be re-fed into centrifuge process,
Take supernatant；The supernatant is sent in vacuum decker, is concentrated into the 1/6 of original volume and is obtained abalone internal organ enzymolysis concentrate；
（5）Chromatography
By step（4）In obtain abalone internal organ enzymolysis concentrate using GE companies Sephadex G-100 gel chromatographies, obtain
Abalone internal organ polysaccharide；
（6）Abalone brewing grape wine
It is raw material to choose new fresh grape, and according to conventional preparation technology wine production raw material is made；In wine production raw material
In by weight be 5:1；Add step（5）Obtained abalone internal organ polysaccharide, boils, and is cooled to 38 DEG C, adds wine production
Wheat koji of the raw material with the 4% of many sugar weights of abalone internal organ, and the carbohydrase of 300 units is added by every gram of wine production raw material, enter
Row saccharification, is cooled to 30 DEG C, is inoculated with into OAU-1 bacterial strains, and the inoculum concentration of OAU-1 bacterial strains is wine production raw material and abalone internal organ
The 0.5% of many sugar weights, is then fed into being fermented in the fermentation tank that sky disappears；Fermentation ends are squeezed, and mash is obtained；By wine with dregs
Liquid carries out filtration clarification, and then Jing decocts wine, and storage is blent, and health care abalone grape wine of the alcoholic strength in 20% (v/v) is obtained.
A kind of health care abalone preparation of wine that the present embodiment is provided, its rational technology operates feasible, Yi Shi
Existing large-scale production.Health care abalone grape wine prepared by it is in good taste, rich in abalone internal organ polysaccharide functional components, Neng Gouzeng
Strong body immunity.
Embodiment 3
A kind of health care abalone preparation of wine, it is through following steps：
（1）The pretreatment of raw material
The abalone internal organ for choosing fresh free from extraneous odour are raw material, are sent into after cleaning up in disintegrating machine, add 3 times of abalone viscera weights
Water, crush, homogenate, obtain abalone internal organ slurries；
（2）Enzymolysis
By step（1）In the abalone internal organ slurries that obtain add the compound protease of pepsin and flavor protease composition, its
In, pepsic addition is the 0.3% of abalone viscera weights, and the addition of flavor protease is 0.2%, controls temperature 30
DEG C, enzymolysis time 20h obtains abalone internal organ enzymolysis liquid；
（3）Go out enzyme
By step（2）In the abalone internal organ enzymolysis liquid that obtains be heated to 90 DEG C, holding is gone out enzyme 10min, the abalone internal organ of the enzyme that must go out
Enzymolysis liquid；
（4）Concentration
By step（3）In the abalone internal organ enzymolysis liquid of the enzyme that goes out that obtains naturally cool to room temperature, be re-fed into centrifuge process,
Take supernatant；The supernatant is sent in vacuum decker, is concentrated into the 1/8 of original volume and is obtained abalone internal organ enzymolysis concentrate；
（5）Chromatography
By step（4）In obtain abalone internal organ enzymolysis concentrate using GE companies Sephadex G-100 gel chromatographies, obtain
Abalone internal organ polysaccharide；
（6）Abalone brewing grape wine
It is raw material to choose new fresh grape, and according to conventional preparation technology wine production raw material is made；In wine production raw material
In by weight be 2:1；Add step（5）Obtained abalone internal organ polysaccharide, boils, and is cooled to 28 DEG C, adds wine production
Wheat koji of the raw material with the 3.5% of many sugar weights of abalone internal organ, and the carbohydrase of 80 units is added by every gram of wine production raw material,
It is saccharified, is cooled to 20 DEG C, be inoculated with into OAU-1 bacterial strains, the inoculum concentration of OAU-1 bacterial strains is in wine production raw material and abalone
The 0.1% of dirty many sugar weights, is then fed into being fermented in the fermentation tank that sky disappears；Fermentation ends are squeezed, and mash is obtained；Will
Mash carries out filtration clarification, and then Jing decocts wine, and storage is blent, and health care abalone grape wine of the alcoholic strength in 8% (v/v) is obtained.
A kind of health care abalone preparation of wine that the present embodiment is provided, its rational technology operates feasible, Yi Shi
Existing large-scale production.Health care abalone grape wine prepared by it is in good taste, rich in abalone internal organ polysaccharide functional components, Neng Gouzeng
Strong body immunity.
Claims (3)
1. a kind of health care abalone preparation of wine, it is characterised in that：It is through following steps：
（1）The pretreatment of raw material
The abalone internal organ for choosing fresh free from extraneous odour are raw material, in sending into disintegrating machine, add the water of 3～10 times of abalone viscera weights,
Broken, homogenate obtains abalone internal organ slurries；
（2）Enzymolysis
By step（1）In the abalone internal organ slurries that obtain add the compound protease of abalone viscera weights 0.5～5%, control temperature
30～50 DEG C, 4～20h of enzymolysis time obtains abalone internal organ enzymolysis liquid；
（3）Go out enzyme
By step（2）In the abalone internal organ enzymolysis liquid that obtains be heated to 90～95 DEG C, holding is gone out 10～30min of enzyme, and must go out enzyme
Abalone internal organ enzymolysis liquid；
（4）Concentration
By step（3）In the abalone internal organ enzymolysis liquid of the enzyme that goes out that obtains naturally cool to room temperature, be re-fed into centrifuge process,
Take supernatant；The supernatant is sent in vacuum concentration equipment, be concentrated into original volume 1/6～1/8 abalone internal organ enzymolysis is dense
Contracting liquid；
（5）Chromatography
By step（4）In the abalone internal organ enzymolysis concentrate that obtains using Sephadex G-100 gel chromatographies, obtain abalone internal organ
Polysaccharide；
（6）Abalone brewing grape wine
It is raw material to choose new fresh grape, and according to conventional preparation technology wine production raw material is made；In wine production raw material
Middle addition step（5）Obtained abalone internal organ polysaccharide, boils, and is cooled to 28～38 DEG C, adds wheat koji and carbohydrase to be saccharified；
20～30 DEG C are cooled to, are inoculated with into OAU-1 bacterial strains, sent in the fermentation tank that sky disappears and fermented；Fermentation ends are squeezed, system
Obtain mash；Mash is carried out into filtration clarification, then Jing decocts wine, storage is blent, and health care of the alcoholic strength in 8～20% (v/v) is obtained
Abalone grape wine.
2. a kind of health care abalone preparation of wine according to claim 1, it is characterised in that：Described compound egg
White enzyme is one or more of bromelain, pepsin, neutral proteinase and flavor protease.
3. a kind of health care abalone preparation of wine according to claim 1, it is characterised in that：Described grape wine
Brewing materials are 2～5 with the weight ratio of abalone internal organ polysaccharide:1；The addition of described wheat koji is wine production raw material and Bao
The 3.5～4% of many sugar weights of fish guts；The addition of described carbohydrase is 80～300 units/gram wine production raw materials；Institute
The inoculum concentration of the OAU-1 bacterial strains stated is the 0.1～0.5% of wine production raw material and many sugar weights of abalone internal organ.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611082527.9A CN106635589A (en) | 2016-11-30 | 2016-11-30 | Preparation method of health-care abalone wine |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611082527.9A CN106635589A (en) | 2016-11-30 | 2016-11-30 | Preparation method of health-care abalone wine |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106635589A true CN106635589A (en) | 2017-05-10 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611082527.9A Pending CN106635589A (en) | 2016-11-30 | 2016-11-30 | Preparation method of health-care abalone wine |
Country Status (1)
| Country | Link |
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Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102559443A (en) * | 2012-02-14 | 2012-07-11 | 中国海洋大学 | Heath-function sea cucumber yellow wine |
-
2016
- 2016-11-30 CN CN201611082527.9A patent/CN106635589A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102559443A (en) * | 2012-02-14 | 2012-07-11 | 中国海洋大学 | Heath-function sea cucumber yellow wine |
Non-Patent Citations (2)
| Title |
|---|
| 程婷婷: "鲍鱼脏器多糖的提取、分离纯化以及结构的研", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
| 贺帅: "北方黄酒的功能性成分分析及保健牡蛎黄酒的研制", 《中国优秀硕士学位论文全文数据库 工程科技Ⅰ辑》 * |
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Application publication date: 20170510 |
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