CN107927743A - A kind of preparation method for the ferment of sobering up that kudzuvine root juice that ferments obtains - Google Patents

A kind of preparation method for the ferment of sobering up that kudzuvine root juice that ferments obtains Download PDF

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Publication number
CN107927743A
CN107927743A CN201711090537.1A CN201711090537A CN107927743A CN 107927743 A CN107927743 A CN 107927743A CN 201711090537 A CN201711090537 A CN 201711090537A CN 107927743 A CN107927743 A CN 107927743A
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kudzuvine root
root juice
ferment
sobering
preparation
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付桂明
万茵
罗成
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Nanchang University
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Nanchang University
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23LFOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23VINDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Life Sciences & Earth Sciences (AREA)
  • Mycology (AREA)
  • Health & Medical Sciences (AREA)
  • Nutrition Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Non-Alcoholic Beverages (AREA)

Abstract

The present invention relates to field of food health care, more particularly to a kind of preparation method of the ferment of sobering up for kudzuvine root juice acquisition of fermenting.Using pueraria lobata as raw material, amylase is added after boiling gelatinization liquefaction, then by controlling the inoculum concentration of lactobacillus plantarum FCJX 102 (lactobacillus plantarum FCJX 102), under different fermentation temperatures and time, obtain the ferment of sobering up with peculiar flavour.The invention has the advantages that:(1) rich in nutrition content, has unique flavor, overcomes the original mouthfeel bitterness of Chinese herbal medicine, the weakness that smell is pungent;(2) composition of gut flora is adjusted to a certain extent, maintains microecological balance, is improved the function of intestines and stomach, is improved immunity of organisms;(3) acetaldehyde dehydrogenase content is high, has the effect of more preferable Soboring-up liver-protecting;(4) step is simple, and operation is flexible, time saving and energy saving, and shelf-life durations length, largely reduces the waste of resource in enterprise's processing, improve market competitiveness of enterprises.

Description

A kind of preparation method for the ferment of sobering up that kudzuvine root juice that ferments obtains
Technical field
The present invention relates to field of food health care, more particularly to a kind of preparation side of the ferment of sobering up for kudzuvine root juice acquisition of fermenting Method.
Background technology
Since ancient times, wine is exactly one of indispensable drink in human lives, it through China culture in 5,000 years In long stream, occupy critical role in the production and living of the mankind.With the development of the times, spirits culture is also constantly developing, From the white wine of the various odor types of the yellow rice wine of 3,000 years till now;From the wine table to quench one's thirst by now the culture of ancient times, none non-disclosre The of long standing and well established of wine and its unique status.It is suitable drink can with dispelling fatigue and it is nervous, drive away cold, relaxing tendons and activating collaterals, Boost metabolism, High-density Lipoprotein-cholesterol can also be improved, reduce insulin resistance etc..However, excessive consumption of alcohol Health can be caused greatly to damage, such as cause alcoholism and hepatic disease, induce alcoholic hepatitis, hepatic sclerosis.Wine Essence also to the toxic effect of reproduction cell, induces fetal congenital deformity.In addition excessive consumption of alcohol induces traffic accident and violence row For global traffic accident and operating irregularity 1/3 are above is as caused by excessive drinking.Therefore, research and development one kind can effectively relieve the effect of alcohol The product of protect liver is of great significance the health for protecting people.
The health food of current existing relieving alcoholism and protecting liver is varied, is to add a variety of enzymes, the food materials of decomposition ethanol mostly And the dairy products of fermentation are processed to obtain, the most common enzyme that relieves the effect of alcohol is acetaldehyde dehydrogenase, but the health care of existing relieving alcoholism and protecting liver is eaten The preparation for processing complex steps of product, malfunction is time-consuming and laborious, to the health food of enterprise's production and processing relieving alcoholism and protecting liver During cause a degree of wasting of resources.
The content of the invention
The present invention provides a kind of preparation method for the ferment of sobering up that kudzuvine root juice that ferments obtains.
The technical problem that the present invention solves is numerous for the health food preparation for processing step of existing relieving alcoholism and protecting liver Trivial, malfunction is time-consuming and laborious, easily causes the wasting of resources.
Another technical problem that the present invention solves is bad for the beverage for dispelling alcoholic intoxication mouthfeel of existing relieving alcoholism and protecting liver, and dispelling effects of alcohol is not It is good.
To solve above technical problem, its technical solution is:
That above-mentioned fermentation kudzuvine root juice process utilizes is the lactobacillus plantarum FCJX 102 for producing acetaldehyde dehydrogenase (lactobacillus plantarum FCJX 102), is preserved in China typical culture collection on June 16th, 2017 Center, preserving number are CCTCC NO:M2017340, depositary institution address:Wuhan, China Wuhan University.
A kind of preparation method of the anti-alcoholism drink obtained using the fermentation kudzuvine root juices of lactobacillus plantarum FCJX 102, it prepares step Suddenly it is:
(1) pretreatment of raw material:Pueraria lobata is crushed to below 20 mesh;
(2) pueraria lobata boiling gelatinization liquefaction:Press pueraria lobata dry mass and add 5-15 times of water, add the 0.1% of pueraria lobata aqueous solution The thermostable α-amylase of quality, 100 DEG C of boiling gelatinization 40~60min of liquefaction, 100 mesh filter-cloth filterings, collect filtrate, obtain Pueraria lobota Root juice A;
(3) sterilizing of kudzuvine root juice:The kudzuvine root juice A obtained in step (2) is adjusted into pH to 6.2~6.4, in 115 DEG C of conditions Lower sterilizing 30min, cooling, it is stand-by to obtain kudzuvine root juice finished product;
(4) learn from else's experience the lactobacillus bacterium powder after frozen dried, be inoculated into the amount of culture medium 1% (w/v) through 115 DEG C of sterilizings In 30min and 12% skimmed milk power culture medium being cooled to room temperature, after cultivating 24h under the conditions of 37 DEG C of constant temperature and humidity, obtain To a kind of lactobacillus seed liquor;
(5) it is inoculated with:The lactobacillus seed liquor that step (4) is obtained is with the inoculum concentration of 1~5% (w/v) of kudzuvine root juice finished product The kudzuvine root juice finished product obtained in inoculation step (3);
(6) fermentation and after-ripening:Kudzuvine root juice finished product after step (5) is inoculated with is transferred to 4 in 37 DEG C of 14~48h of ferment at constant temperature DEG C after-ripening 2-3 days, obtains fermenting-ripening liquid, that is, ferment of sobering up.
The water is pure water.
The thermostable α-amylase of 0.1% mass of pueraria lobata aqueous solution described in step (2), 0.1% is percentage composition.
Lactobacillus is lactobacillus plantarum FCJX 102 described in step (4).
12% skimmed milk power culture medium described in step (4), 12% is the percentage composition of skimmed milk power.
The amount of culture medium 1% (w/v) described in step (4), 1% is percentage composition.
1~5% (w/v) of kudzuvine root juice finished product described in step (5), 1~5% is percentage composition.
Viable lactic acid bacteria content 10 in ferment provided by the invention of sobering up6A/mL, is more than 6U/ containing acetaldehyde dehydrogenase 100mL, 6 months shelf-lifves.
The ferment advantage of sobering up that a kind of fermentation kudzuvine root juice provided in the present invention obtains is:
(1) rich in nutrition content, has unique flavor, overcomes the original mouthfeel bitterness of Chinese herbal medicine, smell is pungent Weakness;
(2) composition of gut flora is adjusted to a certain extent, maintains microecological balance, is improved the function of intestines and stomach, is carried High immunity of organisms;
(3) acetaldehyde dehydrogenase content is high, has the effect of more preferable Soboring-up liver-protecting;
(4) step is simple, and operation is flexible, time saving and energy saving, and shelf-life durations length, largely reduces enterprise's processing The waste of middle resource, improves market competitiveness of enterprises.
Embodiment
The present invention is further detailed with reference to embodiment.
Embodiment 1
(1) pretreatment of raw material:Pueraria lobata is crushed to below 20 mesh;
(2) pueraria lobata boiling gelatinization liquefaction:Press pueraria lobata dry mass and add 5 times of water, add 0.1% mass of pueraria lobata aqueous solution Thermostable α-amylase, 100 DEG C of boilings gelatinization liquefaction 40min, 100 mesh filter-cloth filterings, collect filtrate, obtain kudzuvine root juice A;
(3) sterilizing of kudzuvine root juice:The kudzuvine root juice A obtained in step (2) is adjusted into pH to 6.2~6.4, in 115 DEG C of conditions Lower sterilizing 30min, cooling, obtains kudzuvine root juice finished product, stand-by;
(4) learn from else's experience 102 bacterium powders of lactobacillus plantarum FCJX after frozen dried, connect with the inoculum concentration of culture medium 1% (w/v) Kind to in through 115 DEG C of sterilizing 30min and the 12% skimmed milk power culture medium that is cooled to room temperature, in 37 DEG C of constant temperature and humidity condition After lower culture 24h, the seed liquor of lactobacillus plantarum FCJX 102 is obtained;
(5) it is inoculated with:The lactobacillus plantarum FCJX 102 that step (4) is obtained is with the inoculation of 1% (w/v) of kudzuvine root juice finished product Amount is inoculated into the kudzuvine root juice finished product in step (3);
(6) fermentation and after-ripening:Kudzuvine root juice finished product after step (5) is inoculated with is in 37 DEG C of ferment at constant temperature 14h, after being transferred to 4 DEG C Ripe 3 days, fermenting-ripening liquid is obtained, that is, ferment of sobering up.
Embodiment 2
(1) pretreatment of raw material:Pueraria lobata is crushed to below 20 mesh;
(2) pueraria lobata boiling gelatinization liquefaction:Press pueraria lobata dry mass and add 10 times of water, add 0.1% matter of pueraria lobata aqueous solution The thermostable α-amylase of amount, 100 DEG C of boiling gelatinization liquefaction 50min, 100 mesh filter-cloth filterings, collect filtrate, obtain kudzuvine root juice A;
(3) sterilizing of kudzuvine root juice:The kudzuvine root juice A obtained in step (2) is adjusted into pH to 6.2~6.4, in 115 DEG C of conditions Lower sterilizing 30min, cooling, it is stand-by to obtain kudzuvine root juice finished product;
(4) learn from else's experience 102 bacterium powders of lactobacillus plantarum FCJX after frozen dried, be inoculated with culture medium 1% (w/v) inoculum concentration To in through 115 DEG C of sterilizing 30min and the 12% skimmed milk power culture medium that is cooled to room temperature, trained under the conditions of 37 DEG C of constant temperature and humidity After supporting 24h, the seed liquor of lactobacillus plantarum FCJX 102 is obtained;
(5) it is inoculated with:The lactobacillus plantarum FCJX 102 that step (4) is obtained is with the inoculum concentration of kudzuvine root juice finished product 3% (w/v) The kudzuvine root juice finished product obtained in inoculation step (3);
(6) fermentation and after-ripening:Kudzuvine root juice finished product after step (5) is inoculated with is in 37 DEG C of ferment at constant temperature 28h, after being transferred to 4 DEG C Ripe 2.5 days, fermenting-ripening liquid is obtained, that is, ferment of sobering up.
Embodiment 3
(1) pretreatment of raw material:Pueraria lobata is crushed to below 20 mesh;
(2) pueraria lobata boiling gelatinization liquefaction:Press pueraria lobata dry mass and add 10 times of water, add 0.1% matter of pueraria lobata aqueous solution The thermostable α-amylase of amount, 100 DEG C of boiling gelatinization liquefaction 60min, 100 mesh filter-cloth filterings, collect filtrate, obtain kudzuvine root juice A;
(3) sterilizing of kudzuvine root juice:The kudzuvine root juice A obtained in step (2) is adjusted into pH to 6.2~6.4, in 115 DEG C of conditions Lower sterilizing 30min, it is stand-by to obtain kudzuvine root juice finished product after cooling;
(4) learn from else's experience 102 bacterium powders of lactobacillus plantarum FCJX after frozen dried, be inoculated with culture medium 1% (w/v) inoculum concentration To in through 115 DEG C of sterilizing 30min and the 12% skimmed milk power culture medium that is cooled to room temperature, trained under the conditions of 37 DEG C of constant temperature and humidity After supporting 24h, the seed liquor of lactobacillus plantarum FCJX 102 is obtained;
(5) it is inoculated with:The lactobacillus plantarum FCJX 102 that step (4) is obtained is with the inoculum concentration of kudzuvine root juice finished product 5% (w/v) The kudzuvine root juice finished product obtained in inoculation step (3);
(6) fermentation and after-ripening:Kudzuvine root juice finished product after step (5) is inoculated with is in 37 DEG C of ferment at constant temperature 48h, after being transferred to 4 DEG C Ripe 2 days, fermenting-ripening liquid is obtained, that is, ferment of sobering up.
Embodiment 4
(1) pretreatment of raw material:Pueraria lobata is crushed to below 20 mesh;
(2) pueraria lobata boiling gelatinization liquefaction:Press pueraria lobata dry mass and add 15 times of water, add 0.1% matter of pueraria lobata aqueous solution The thermostable α-amylase of amount, 100 DEG C of boiling gelatinization liquefaction 60min, 100 mesh filter-cloth filterings, collect filtrate, obtain kudzuvine root juice A;
(3) sterilizing of kudzuvine root juice:The kudzuvine root juice A obtained in step (2) is adjusted into pH to 6.2~6.4, in 115 DEG C of conditions Lower sterilizing 30min, cooling, obtains kudzuvine root juice finished product, stand-by;
(4) learn from else's experience 102 bacterium powders of lactobacillus plantarum FCJX after frozen dried, be inoculated with culture medium 1% (w/v) inoculum concentration To in through 115 DEG C of sterilizing 30min and the 12% skimmed milk power culture medium that is cooled to room temperature, trained under the conditions of 37 DEG C of constant temperature and humidity After supporting 24h, the seed liquor of lactobacillus plantarum FCJX 102 is obtained;
(5) it is inoculated with:The lactobacillus plantarum FCJX 102 that step (4) is obtained is with the inoculum concentration of kudzuvine root juice finished product 3% (w/v) It is inoculated into the kudzuvine root juice finished product obtained in step (3);
(6) fermentation and after-ripening:Kudzuvine root juice finished product after step (5) is inoculated with is in 37 DEG C of ferment at constant temperature 28h, after being transferred to 4 DEG C Ripe 2 days, fermenting-ripening liquid is obtained, that is, ferment of sobering up.

Claims (7)

  1. A kind of 1. preparation method for the ferment of sobering up that kudzuvine root juice that ferments obtains:It is characterized in that:
    Preparation process is as follows:
    (1) pretreatment of raw material:Pueraria lobata is crushed to below 20 mesh;
    (2) pueraria lobata boiling gelatinization liquefaction:Press pueraria lobata dry mass and add 5-15 times of water, add 0.1% mass of pueraria lobata aqueous solution Thermostable α-amylase, 100 DEG C of boilings gelatinization 40~60min of liquefaction, 100 mesh filter-cloth filterings, collect filtrate, obtain kudzuvine root juice A;
    (3) sterilizing of kudzuvine root juice:The kudzuvine root juice A obtained in step (2) is adjusted into pH to 6.2~6.4, is gone out under the conditions of 115 DEG C Bacterium 30min, cooling, it is stand-by to obtain kudzuvine root juice finished product;
    (4) learn from else's experience the lactobacillus bacterium powder after frozen dried, be inoculated into the amount of culture medium 1% (w/v) through 115 DEG C of sterilizing 30min And in the skimmed milk power culture medium of 12% be cooled to room temperature, after cultivating 24h under the conditions of 37 DEG C of constant temperature and humidity, obtain one kind Lactobacillus seed liquor;
    (5) it is inoculated with:The lactobacillus seed liquor that step (4) is obtained is inoculated with the inoculum concentration of 1~5% (w/v) of kudzuvine root juice finished product The kudzuvine root juice finished product obtained in step (3);
    (6) fermentation and after-ripening:Kudzuvine root juice finished product after step (5) is inoculated with is in 37 DEG C of 14~48h of ferment at constant temperature, after being transferred to 4 DEG C Ripe 2-3 days, fermenting-ripening liquid is obtained, that is, ferment of sobering up.
  2. 2. the preparation method for the ferment of sobering up that a kind of kudzuvine root juice that ferments as claimed in claim 1 obtains, it is characterized in that:
    Lactobacillus described in step (4) is (the lactobacillus plantarum FCJX of lactobacillus plantarum FCJX 102 102) China typical culture collection center, is preserved on June 16th, 2017, preserving number is CCTCC NO:M2017340.
  3. 3. the preparation method for the ferment of sobering up that a kind of kudzuvine root juice that ferments as claimed in claim 1 obtains, it is characterized in that:Prepare step Water is pure water described in rapid.
  4. 4. the preparation method for the ferment of sobering up that a kind of kudzuvine root juice that ferments as claimed in claim 1 obtains, it is characterized in that:Step (2) thermostable α-amylase of 0.1% mass of pueraria lobata aqueous solution described in, 0.1% is percentage composition.
  5. 5. the preparation method for the ferment of sobering up that a kind of kudzuvine root juice that ferments as claimed in claim 1 obtains, it is characterized in that:Step (4) 12% skimmed milk power culture medium described in, 12% is the percentage composition of skimmed milk power.
  6. 6. the preparation method for the ferment of sobering up that a kind of kudzuvine root juice that ferments as claimed in claim 1 obtains, it is characterized in that:Step (4) amount of culture medium 1% described in (w/v), 1% is percentage composition.
  7. 7. the preparation method for the ferment of sobering up that a kind of kudzuvine root juice that ferments as claimed in claim 1 obtains, it is characterized in that:Step (5) 1~5% (w/v) of kudzuvine root juice finished product described in, 1~5% is percentage composition.
CN201711090537.1A 2017-11-08 2017-11-08 A kind of preparation method for the ferment of sobering up that kudzuvine root juice that ferments obtains Pending CN107927743A (en)

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111406863A (en) * 2020-05-07 2020-07-14 浙江省农业科学院 Preparation method of grapefruit and kudzu root fermented beverage with sobering-up effect
CN113826886A (en) * 2021-09-06 2021-12-24 傅美静 Radix puerariae enzyme and preparation method thereof
CN114886060A (en) * 2022-04-25 2022-08-12 陕西五丁生物科技有限公司 Sobering herbal beverage based on radix puerariae enzyme and production process thereof

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CN104621662A (en) * 2015-01-26 2015-05-20 江苏大学 Method for preparing functional drink by utilizing radix puerariae-lactic acid bacteria mixed fermentation
CN106266105A (en) * 2016-09-05 2017-01-04 三株福尔制药有限公司 A kind of probiotics fermention Chinese medicine composition of Antialcoholic liver-protecting and its preparation method and application
CN106858577A (en) * 2017-04-11 2017-06-20 姜红成 Prepare the fermentation composition and preparation method of the plant enzyme with liver protecting, the fatty subtotal hepatectomy of regulation
CN107568736A (en) * 2017-07-04 2018-01-12 劲膳美食品股份有限公司 Dealcoholic sobering-up medical science formula food

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CN104621662A (en) * 2015-01-26 2015-05-20 江苏大学 Method for preparing functional drink by utilizing radix puerariae-lactic acid bacteria mixed fermentation
CN106266105A (en) * 2016-09-05 2017-01-04 三株福尔制药有限公司 A kind of probiotics fermention Chinese medicine composition of Antialcoholic liver-protecting and its preparation method and application
CN106858577A (en) * 2017-04-11 2017-06-20 姜红成 Prepare the fermentation composition and preparation method of the plant enzyme with liver protecting, the fatty subtotal hepatectomy of regulation
CN107568736A (en) * 2017-07-04 2018-01-12 劲膳美食品股份有限公司 Dealcoholic sobering-up medical science formula food

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Title
罗成等: "植物乳杆菌FCJX 102和嗜酸乳杆菌FCJX 104共发酵山楂-葛根汁产乙醛脱氢酶", 《食品与发酵工业》 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111406863A (en) * 2020-05-07 2020-07-14 浙江省农业科学院 Preparation method of grapefruit and kudzu root fermented beverage with sobering-up effect
CN113826886A (en) * 2021-09-06 2021-12-24 傅美静 Radix puerariae enzyme and preparation method thereof
CN114886060A (en) * 2022-04-25 2022-08-12 陕西五丁生物科技有限公司 Sobering herbal beverage based on radix puerariae enzyme and production process thereof

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Application publication date: 20180420