CN106632710B - Radix Codonopsis homogeneous polysaccharide with anti-gastric-ulcer and preparation method and application - Google Patents
Radix Codonopsis homogeneous polysaccharide with anti-gastric-ulcer and preparation method and application Download PDFInfo
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- CN106632710B CN106632710B CN201610889548.5A CN201610889548A CN106632710B CN 106632710 B CN106632710 B CN 106632710B CN 201610889548 A CN201610889548 A CN 201610889548A CN 106632710 B CN106632710 B CN 106632710B
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0051—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid beta-D-Fructofuranans, e.g. beta-2,6-D-fructofuranan, i.e. levan; Derivatives thereof
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- A61K31/00—Medicinal preparations containing organic active ingredients
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- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/34—Campanulaceae (Bellflower family)
- A61K36/344—Codonopsis
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Abstract
The invention discloses a kind of Radix Codonopsis homogeneous polysaccharide with anti-gastric-ulcer, which is 3000, is beta-D-fructofuranose residue with straight chain homogeneous polysaccharide made of β-(2 → 1)-glucosides key connection.Separating and extracting process includes the following steps：（1）, extraction Radix Codonopsis total starches；（2）, Codonopsis pilosula polysaccharide ultra-filtration and separation.Application of the Radix Codonopsis homogeneous polysaccharide in treating Gastric Ulcer Treatment.
Description
Technical field
The present invention relates to the preparation methods of active ingredient of Chinese herbs, specially the Radix Codonopsis homogeneous polysaccharide with anti-ulcer effect
Isolation and purification method and its anti-ulcer effect.
Background technology
Radix Codonopsis（Radix Codonopsis）For campanulaceae of the genus codonopsis, have effects that tonifying middle-Jiao and Qi, strengthening spleen and tonifying lung,
It is clinically used for spleen and lung weakness, shortness of breath and palpitation, anorexia and loose stool, virtual asthma cough, interior heat disappears the diseases such as cough.Radix Codonopsis mainly contains polysaccharide, soap
The ingredients such as glycosides, triterpene, alkaloid, wherein polyoses content are maximum, are one of the important component of Radix Codonopsis.Modern pharmacological studies have shown that
Codonopsis pilosula polysaccharide has preferable biology in many aspects such as anti-aging, adjusting immunity of organisms, anti anoxia, resisting stress, anti-oxidant
Activity.
But the pharmacological research of document report is all directed to Radix Codonopsis total starches mostly, to forming, uniform, structure is specific at present
The pharmacological activity and its Mechanism Study of Codonopsis pilosula polysaccharide are almost without relevant report.
Invention content
The present invention solves the technical problem of by being classified by molecular weight to Codonopsis pilosula polysaccharide, drug effect is then carried out
Screening active ingredients are learned, a kind of active polysaccharide for having and forming uniform anti-gastric-ulcer is obtained.
The present invention adopts the following technical scheme that realization：
A kind of Radix Codonopsis homogeneous polysaccharide with anti-gastric-ulcer, general structure are as shown in Figure 6.
The polysaccharide molecular weight is 3000, is beta-D-fructofuranose residue with straight chain made of β-(2 → 1)-glucosides key connection
Homogeneous polysaccharide.
The separating and extracting process of the above-mentioned Radix Codonopsis homogeneous polysaccharide with anti-gastric-ulcer, includes the following steps：
（1）, extraction Radix Codonopsis total starches；
The patent obtained according to early period（200710062520.5）, extract Radix Codonopsis total starches.
（2）, Codonopsis pilosula polysaccharide ultra-filtration and separation
Radix Codonopsis total starches distilled water is diluted 10~30 times, heating water bath is to 20 DEG C~90 DEG C fully dissolvings, in 10 DEG C
12~72 hours are stood below, is concentrated into 1~3g/mL, with 60%~95% ethyl alcohol alcohol precipitation, 4~24 hours is stood, centrifuges, sink
Shallow lake drying；Repeat aforesaid operations 1-3 times；Radix Codonopsis total starches solution successively passes through the filter membrane and 1000-10000 of ten thousand molecular weight of 1-10
Molecular weight filter membrane obtains Radix Codonopsis homogeneous polysaccharide, is denoted as CPS-A.
The Structural Identification of Codonopsis pilosula polysaccharide CPS-A is as follows：
Purity and molecule measuring are carried out using High Performance Gel Permeation Chromatography to the Codonopsis pilosula polysaccharide CPS-A that the present invention obtains
It is fixed：
Chromatographic condition：Shodex OHpak SB-804 chromatographic columns, mobile phase are ultra-pure water, flow velocity 0.3mL/min, detection
Device is differential refraction detector；By the appropriate water dissolutions of Codonopsis pilosula polysaccharide CPS-A, then sample introduction is analyzed, and analysis result is in single right
Claim peak, illustrates that the group is divided into the uniform polysaccharide of molecular weight（As shown in Figure 1）.
Structure determination is carried out to the Codonopsis pilosula polysaccharide CPS-A that the present invention obtains：
CPS-A differentiates through purity, it was demonstrated that has been single composition, can carry out structural analysis.
CPS-A is through complete hydrolysis, gas chromatography-mass spectrography analysis, it is known that and it is fructose, with1H and13CNMR is further
Analysis.
Above-mentioned analytical proof：CPS-A molecular weight is 3000, is that beta-D-fructofuranose residue is connected with β-(2 → 1)-glycosidic bond
Straight chain homogeneous polysaccharide made of connecing.
Application of the Radix Codonopsis homogeneous polysaccharide in treating Gastric Ulcer Treatment, following pharmacodynamic experiments confirm that CPS-A has anti-stomach
The pharmacological activity of ulcer.
Codonopsis pilosula polysaccharide CP-A is as follows to the inhibiting effect of alcohol induced acute gastric ulcer rat in the present invention：
SD rats are randomly divided into 6 groups, every group of 10 rats：Blank group, model group, the high, medium and low dosage of Codonopsis pilosula polysaccharide
Group, positive control（Bismuth Potassium Citrate）Group.Blank group is according to 10mL/kg gavage physiological saline, model group gavage distilled water 10mL/
Kg, the high, medium and low dosage group of Codonopsis pilosula polysaccharide is according to Codonopsis pilosula polysaccharide 0.8g/kg, 0.4g/kg, 0.2g/kg gavage.Positive drug group is pressed
According to Bismuth Potassium Citrate 100mg/kg gavages.One time a day, after continuous 7 days, animal is deprived of food but not water 24 hours, and the 8th day starts to be administered
Preceding taboo water.After administration 3 hours, in addition to blank group, each group gives 70% ethyl alcohol gavages of 1mL.Blank group gives 1mL distilled water.Modeling 1
After hour, yellow Jackets 45mg/kg anesthetized rats are injected intraperitoneally, abdominal aorta blood drawing execution animal after 5 minutes.It takes out
Stomach cuts off stomach wall along greater curvature, observes stomach lining, carry out the measurement of ulcer index, calculates gastric mucosal damage index, and then one
Half gastric tissue is put into 10% neutral formalin solution fixed, 10% tissue homogenate of half preparation, for detecting in gastric tissue
MPO, SOD, GSH-Px activity and MDA, NO content.All data use ± s to indicate, the significance test of multigroup data difference
Using one-way analysis of variance.
As a result such as Fig. 7, Fig. 8, Tables 1 and 2.
Fig. 7 shows that normal rats stomach lining is smooth, without bleeding.Model group rats stomach lining has serious item
Strand bleeding lesions, CPS-A high dose groups and middle dose group bleeding are less.
Fig. 8 shows A under light microscopic（Normal group）Gastric Mucosal Cells marshalling, fine and close, rule has no that meronecrosis falls off
It is abnormal, 0 grade of degree of injury（Mucosal lesion grading is carried out according to Lacy methods）.B（Model group）Gastric Mucosal Cells are bad extensively
It extremely falls off, karyopyknosis, cytoplasm cracking, cell arrangement is disorderly, and interstitial is loose, and damage is invaded and mucosa cells lower layer, damage
Degree III level.C（CPS-A high dose groups）Stomach lining cells of superficial layer irregular arrangement, submucosa cell arrangement is neat, damage
I grades of degree.D（CPS-A middle dose groups）Gastric Mucosal Cells extensive necrosis falls off, but does not damage to body of gland.II grades of degree of injury.
E（CPS-A low dose groups）Gastric Mucosal Cells layer is thinning, and cell arrangement is loose and extensive necrosis falls off, karyopyknosis, cell
Matter cracks, and damage is invaded and body of gland, degree of injury III level.F（Positive drug Bismuth Potassium Citrate group）Gastric Mucosal Cells are normal,
The arrangement of mucous membrane upper cell is loose irregular, and karyopyknosis does not extend to lower confluent monolayer cells, I grades of degree of injury.
Influences of 1 CPS-A of table to alcohol induced rat gastric ulcer gastric tissue oxidative stress
Table 1 shows that CPS-A can improve SOD and GSH-Px activity in rat gastric tissue in various degree, reduce MPO activity and
NO, MDA content.And rat stomach tissue GSH-Px activity increases with the increase of CPSa dosage, and MDA contents are with CPSa agent
The increase of amount and reduce, MPO activity reduces with the increase of CPSa dosage.
2 rat gastric ulcer index of table and ulcer inhibition rate（±s）
Table 2 shows that Bismuth Potassium Citrate group, CPS-A high doses and middle dose group can significantly reduce rat gastric ulcer index
（Ulcer index measurement is carried out according to Guth methods）.
Description of the drawings
Fig. 1 shows CPS-A graph of molecular weight distribution.
Fig. 2 indicates the gas-chromatography and standard monosaccharide compares figure of CPS-A.
Fig. 3 indicates the infrared chromatogram of CPS-A.
Fig. 4 indicates CPS-A nuclear magnetic resonance1H-NMR spectrum.
Fig. 5 indicates CPS-A's13C-NMR spectrograms.
Fig. 6 indicates the structural formula of CPS-A.
Fig. 7 indicates influences of the CPS-A to alcohol induced rat gastric ulcer gastric tissue form, wherein A indicates normal group, B tables
Representation model group, C indicate Radix Codonopsis CPS-A high dose groups（50mg/kg）, D expression Radix Codonopsis CPS-A middle dose groups（25mg/kg）, E tables
Show Radix Codonopsis CPS-A low dose groups（12.5mg/kg）, F expression positive drug Bismuth Potassium Citrate groups（100mg/kg）.
Fig. 8 shows CPS-A to be sliced light microscopic result to alcohol induced rat gastric ulcer gastric tissue（10×10）, wherein A is indicated
Normal group, B indicates that model group, C indicate Radix Codonopsis CPS-A high dose groups（50mg/kg）, D expression Radix Codonopsis CPS-A middle dose groups
（25mg/kg）, E expression Radix Codonopsis CPS-A low dose groups（12.5mg/kg）, F expression positive drug Bismuth Potassium Citrate groups（100mg/
kg）.
Specific implementation mode
Specific embodiments of the present invention are described in detail below.
Embodiment 1
The preparation of Codonopsis pilosula polysaccharide CPS-A, includes the following steps：
Radix Codonopsis total starches 100g is taken, 2000mL distilled water is added, 80 DEG C dissolve 1 hour, are during which stirred continuously.4 DEG C stand 12
Hour, centrifugation.Supernatant concentration is to 100mL, and 90% ethyl alcohol alcohol precipitation, 4 DEG C stand 12 hours, centrifugation.After precipitation is dry, repetition
Preceding operation, obtains polysaccharide precipitation, and with 3000mL distilled water, 80 DEG C dissolve 1 hour, are during which stirred continuously, and 4 DEG C of standings 12 are small
When, centrifugation, for supernatant successively with 100000 molecular weight films, 5000 molecular weight film ultrafiltration, pressure is 1.5 atmospheric pressure, will be last
It is concentrated by the filtrate of 5000 molecular weight films, it is dry, obtain 36.45g CPS-A.
Embodiment 2
The preparation of Codonopsis pilosula polysaccharide CPS-A, includes the following steps：
Radix Codonopsis total starches 100g is taken, 3000mL distilled water is added, 85 DEG C dissolve 1.5 hours, are during which stirred continuously.4 DEG C of standings
24 hours, centrifugation.Supernatant concentration is to 100mL, and 95% ethyl alcohol alcohol precipitation, 4 DEG C stand 24 hours, centrifugation.After precipitation is dry, repeat
Operation before, obtains polysaccharide precipitation, and with 4000mL distilled water, 85 DEG C dissolve 1.5 hours, are during which stirred continuously, and 4 DEG C stand 24
Hour, centrifugation, for supernatant successively with 100000 molecular weight films, 5000 molecular weight film ultrafiltration, pressure is 2.0 atmospheric pressure, will most
It is concentrated afterwards by the filtrate of 5000 molecular weight films, it is dry, obtain 40.30g CPS-A.
Embodiment 3
The preparation of Codonopsis pilosula polysaccharide CPS-A, includes the following steps：
Radix Codonopsis total starches 100g is taken, 2500mL distilled water is added, 85 DEG C dissolve 1 hour, are during which stirred continuously.4 DEG C stand 24
Hour, centrifugation.Supernatant concentration is to 100mL, and 95% ethyl alcohol alcohol precipitation, 4 DEG C stand 24 hours, centrifugation.After precipitation is dry, repetition
Preceding operation, obtains polysaccharide precipitation, and with 3500mL distilled water, 85 DEG C dissolve 1 hour, are during which stirred continuously, and 4 DEG C of standings 24 are small
When, centrifugation, for supernatant successively with 100000 molecular weight films, 5000 molecular weight film ultrafiltration, pressure is 2.5 atmospheric pressure, will be last
It is concentrated by the filtrate of 5000 molecular weight films, it is dry, obtain 41.50g CPS-A.
The Structural Identification of the Codonopsis pilosula polysaccharide CPS-A of the present embodiment is as follows：
1, saccharic composition decomposition and gas chromatographic analysis
30mg CPS-A are taken, tool plug test tube is put into, the trifluoroacetic acid 2mL of 2mol/L is added, tube sealing, 100 DEG C hydrolyze 12h,
N after taking-up2Drying, it is spare.Derivative is prepared using sugared nitrile acetyl method：After weighing standard monosaccharide and polysaccharide sample hydrolysis respectively
Hydroxylamine hydrochloride 10mg is added in each 10mg of sugar-like, and internal standard inositol 7mg, pyridine 0.5mL in 90 DEG C of heating water bath 30min and vibrate,
Taking-up is cooled to room temperature, and acetic anhydride 0.5mL is added, and continues to heat 30min progress acetylations at 85 DEG C, product is concentrated to dryness, adds
Enter the dissolving of 0.5mL chloroforms, solution after 0.45 membrane filtration, analyzed by 0.2 μ L sample introductions, gas chromatograph.As a result such as Fig. 2 institutes
Show, compared with standard monosaccharide, CPS-A hydrolysates correspond to fructose.
2, infrared absorption spectrum
Sample is measured using KBr pressed disc methods, and the results are shown in Figure 3, and the feature of typical polysaccharide is presented in CPS-A from IR figures
Absorption peak, in wave number 840cm-1Place illustrates to be free of α-glycosidic bond without absorption, and contains β-glycosidic bond.
3, NMR spectrum
1H and13The analysis of CNMR spectrums
Sample is dissolved in D2In O, is measured under room temperature, as a result see Fig. 4 and Fig. 5.Reference literature data, CPS-A's13CNMR spectrum letters
It is number similar to the C signal of Beta-D-Fructopyranose polysaccharide, in conjunction with document glycosidation displacement law, it is known that the fructose in CPS-A is with-β（1,
2）Key connection.It is as follows to each signals assignment：62.0 （C-1）, 103.4（C-2）, 77.0（C-3）, 74.5（C-4）, 81.5
（C-5）, 60.8（C-6）.
It should be noted last that the above examples are only used to illustrate the technical scheme of the present invention and are not limiting, although ginseng
It is described in detail according to the embodiment of the present invention, it will be understood by those of ordinary skill in the art that, to technical scheme of the present invention
It is modified or replaced equivalently, without departure from the spirit and scope of technical scheme of the present invention, claim should all be covered
In protection domain.
Claims (3)
1. a kind of Radix Codonopsis homogeneous polysaccharide with anti-gastric-ulcer, it is characterised in that：General structure is as follows：
；
The polysaccharide molecular weight is 3000, is that beta-D-fructofuranose residue is uniform with straight chain made of β-(2 → 1)-glucosides key connection
Polysaccharide.
2. a kind of separating and extracting process of the Radix Codonopsis homogeneous polysaccharide described in claim 1 with anti-gastric-ulcer, it is characterised in that：
Include the following steps：
（1）, extraction Radix Codonopsis total starches；
（2）, Codonopsis pilosula polysaccharide ultra-filtration and separation
Radix Codonopsis total starches distilled water is diluted 10~30 times, heating water bath is to 20 DEG C~90 DEG C fully dissolvings, in 10 DEG C or less
12~72 hours are stood, 1~3g/mL is concentrated into, with 60%~95% ethyl alcohol alcohol precipitation, stands 4~24 hours, centrifugation, precipitation is done
It is dry；Repeat aforesaid operations 1-3 times；Radix Codonopsis total starches solution successively passes through the filter membrane and 1000-10000 molecules of ten thousand molecular weight of 1-10
Filter membrane is measured, Radix Codonopsis homogeneous polysaccharide is obtained；The polysaccharide molecular weight is 3000, is beta-D-fructofuranose residue with β-(2 → 1)-glucosides
Straight chain homogeneous polysaccharide made of key connection.
3. a kind of application of Radix Codonopsis homogeneous polysaccharide as claimed in claim 1 or 2 in treating Gastric Ulcer Treatment.
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| CN112375157B (en) * | 2020-12-04 | 2022-04-19 | 山西医科大学 | Codonopsis pilosula glucan with immunoregulation effect and preparation method and application thereof |
| CN112625305B (en) * | 2020-12-29 | 2022-06-14 | 山西医科大学 | Codonopsis pilosula fructan compound capable of promoting growth of pediococcus pentosaceus and preparation method and application thereof |
| CN115286721B (en) * | 2022-07-28 | 2023-08-25 | 深圳海创生物科技有限公司 | Active polysaccharide, active polysaccharide composition and application thereof in preparation of products with effect of preventing or treating gastric injury |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101129439A (en) * | 2007-08-01 | 2008-02-27 | 山西医科大学 | Method for extracting codonopsis pilosula polyoses |
| CN101933894A (en) * | 2009-07-03 | 2011-01-05 | 张清 | Protecting colloid for gastroenteric mucosa |
| CN102525901A (en) * | 2012-01-19 | 2012-07-04 | 江西新世纪民星动物保健品有限公司 | Animal codonopsis pilosula polysaccharide oral solution and preparation method thereof |
| CN103145864A (en) * | 2013-01-25 | 2013-06-12 | 兰州瑞业生化科技有限公司 | Codonopsis pilosula uniform polysaccharide CPPib, preparation and application thereof |
-
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- 2016-10-12 CN CN201610889548.5A patent/CN106632710B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101129439A (en) * | 2007-08-01 | 2008-02-27 | 山西医科大学 | Method for extracting codonopsis pilosula polyoses |
| CN101933894A (en) * | 2009-07-03 | 2011-01-05 | 张清 | Protecting colloid for gastroenteric mucosa |
| CN102525901A (en) * | 2012-01-19 | 2012-07-04 | 江西新世纪民星动物保健品有限公司 | Animal codonopsis pilosula polysaccharide oral solution and preparation method thereof |
| CN103145864A (en) * | 2013-01-25 | 2013-06-12 | 兰州瑞业生化科技有限公司 | Codonopsis pilosula uniform polysaccharide CPPib, preparation and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| 一种水溶性党参多糖的分离纯化及结构分析;刘章泉 等，;《基因组学与应用生物学》;20160625;第35卷(第6期);第1294-1299页 * |
| 党参果聚糖的化学结构;叶冠 等，;《中国中药杂志》;20050901;第30卷(第17期);第1338-1340页 * |
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