CN101129439A - Method for extracting codonopsis pilosula polyoses - Google Patents
Method for extracting codonopsis pilosula polyoses Download PDFInfo
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Abstract
The invention discloses an extracting method of codonopsis pilosula polysaccharide, which comprises the following steps: dedusting; cleaning; cutting; removing impurity; refluxing the codonopsis pilosula segment through 80-95% alcohol; doing aqueous extraction; decompressing; condensing until the relative density of drug liquid is 0. 8-1. 2g/ml; placing; cooling; sedimenting through alcohol; adding alcohol into the placed and cooled condensate until the alcohol density is 70-90%; stewing; centrifuging; obtaining the alcohol sediment; dehydrating; drying; obtaining the product with content of the codonopsis pilosula polysaccharide over 70% and extracting rate at 20-30%. The invention simplifies the operation with low cost, which reduces the impurity to possess good appearance, therefore adding the subsequent purifying disposal to clarify the structural component.
Description
Technical field
The present invention relates to the Chinese crude drug extraction of effective components, be specially the extracting method of Radix Codonopsis polysaccharide.
Background technology
Radix Codonopsis (Radix Codonopsis) is a campanulaceae Codonopsis plant, and dry root is used as medicine, but invigorating the spleen and replenishing QI, the spleen invigorating lung benefiting.Be used for deficiency of the spleen and lung, the cardiopalmus of breathing hard, anorexia and loose stool, the dyspnea due to deficiency cough, interior-heat disappears and coughs.
Radix Codonopsis mainly contains (1) saccharide, and polysaccharide, heteropolysaccharide are arranged, and the wherein present fixed 4 kinds of heteropolysaccharide CPI-IV that have mainly are made of in varing proportions glucose, fructose, galactose, mannose, arabinose, xylose and rhamnose.Contain more inulin and fructose in addition.(2) glycoside, ligustrin n-hexyl-β-D-pyranglucoside, ethyl-α-D-fructofuranoside, the I of tangshenoside.(3) micro-alkaloid and nitrogen containing component.(4) sterol and three terpene components.(5) volatile oil and other compositions.
Radix Codonopsis extract--Radix Codonopsis polysaccharide has many aspects biological activity preferably, and toxicological experiment proves that it is an innocuous substance.Modern pharmacological research shows that Radix Codonopsis polysaccharide has effects such as the immunity of organisms of adjusting, defying age, anti-hypoxia, anti-stress, antioxidation.
At present, the Radix Codonopsis polysaccharide extractive technique mainly contains water and lifts from centrifugal separation, decoction and alcohol sedimentation technique etc., but existing extracting method causes in the extract Radix Codonopsis polysaccharide content low because technical parameter is selected unreasonablely.As the patent No. is that 200410012172.7 Chinese patent discloses a kind of water that adopts and lifts from the method that centrifugal separation prepares Radix Codonopsis polysaccharide, and Radix Codonopsis polysaccharide content is only more than 15% in the extract; Number of patent application is the preparation method that 200510012557.8 Chinese patent also discloses a kind of Radix Codonopsis polysaccharide, and in the extract of the yellow powder shape that obtains, Radix Codonopsis polysaccharide content is more than 60%.Simultaneously, the extract that existing extracting method obtains all is the crude product of Radix Codonopsis polysaccharide, owing to extract without being further purified, extract impurity is many, appearance character is not good, polysaccharide component is indeterminate.In addition, existing extracting method only is that index is monitored extraction process usually with the polyoses content, does not investigate extraction ratio, thereby can not embody the scientific and advanced of technology well.
Summary of the invention
The present invention selects unreasonable in order to solve existing Radix Codonopsis polysaccharide extracting method technical parameter, extract Radix Codonopsis polysaccharide content is lower, extraction process is not investigated extraction ratio and not purified, problems such as extract impurity is many, appearance character is not good, polysaccharide component is indeterminate provide a kind of extracting method of Radix Codonopsis polysaccharide.This extracting method can obtain content and reach highly purified Radix Codonopsis polysaccharide more than 70%, and extraction rate reached 20%～30% is carried out further purification process to the Radix Codonopsis polysaccharide crude product simultaneously.
The present invention adopts following technical scheme to realize: the extracting method of Radix Codonopsis polysaccharide, and comprise the steps: that (1) dedusting is clean: medical material Radix Codonopsis flushing with clean water is clean; (2) chopping: the section that Radix Codonopsis is cut into 2～4cm; (3) remove impurity: with the Radix Codonopsis section that cuts, 80%～95% alcohol reflux 2～3 times each 1～2 hour, volatilizes ethanol; (4) water is carried: the medicinal residues after the remove impurity add the distilled water of 8～20 times of medicinal residues weight, and boiling water refluxes 2～3 times, and each 1～2 hour, filter, merging filtrate, being evaporated to relative density of medicine liquid is 0.8～1.2g/ml, places cooling; (5) ethanol precipitation: will place refrigerative concentrated solution adding ethanol to alcohol precipitation concentration is 70%～90%, leaves standstill, centrifugal, obtains pure hypostasis; (6) dehydration: pure hypostasis is washed successively with dehydrated alcohol, acetone, ether; (7) drying: the pure hypostasis vacuum drying after will washing, getting the yellow-white powdery precipitate is Radix Codonopsis polysaccharide extract.
Reflux, extract, solution-ethanol that the present invention is used and concentration (80%～95%) thereof have guaranteed that most of non-polysaccharide composition is dissolved in the extraction solution in the Radix Codonopsis, thereby be beneficial to follow-up concentrating under reduced pressure (impurity too much can produce a large amount of foams), and guaranteed the Radix Codonopsis polysaccharide of high level.The concentrating degree of Radix Codonopsis polysaccharide extracting solution is a key factor that influences the Radix Codonopsis polysaccharide extraction ratio, polysaccharide has a spot of dissolving in the alcoholic solution of low concentration, extracting solution directly adds ethanol and carries out alcohol and analyse without concentrating, ethanol is diluted, concentration of alcohol reduces, make polysaccharide that a spot of dissolving be arranged, polysaccharide extract rate reduces; If concentrate excessively, then make too thickness of extracting solution, polysaccharide forms gelatinous mass with albumen, impurity and solvent easily, thereby has influenced contacting of ethanol and polysaccharide, and separation is made troubles to ethanol precipitation, reduction Radix Codonopsis polysaccharide extraction ratio.The present invention on the bases of a large amount of experiments, optimize determined extracting solution relative density between 0.8～1.2g/ml, thereby can improve the Radix Codonopsis polysaccharide extraction ratio to a certain extent.The ethanol alcohol precipitation concentration that the present invention determines is 70%～90%, not only can guarantee that the Radix Codonopsis polysaccharide composition is precipitated out fully, and the impurity that wraps up during polysaccharide precipitation in this alcohol precipitation concentration scope is less, thereby Radix Codonopsis polysaccharide content is higher.
Adopt sulfuric acid-phynol method to measure total sugar content to the Radix Codonopsis polysaccharide CPS that adopts extracting method of the present invention to obtain, 3,5-dinitrosalicylic acid method is measured contents of monosaccharides, and total sugar content deducts contents of monosaccharides and is Radix Codonopsis polysaccharide content.With the glucose is reference substance, and the result is as follows for the Radix Codonopsis polysaccharide assay:
| The Radix Codonopsis polysaccharide lot number | Polyoses content (%) |
| 060222 060626 061003 | 79.37 79.76 80.54 |
The result shows that polyoses content reaches more than 70% with glucose meter in the Radix Codonopsis polysaccharide extract.
Adopt the efficient gel permeation chromatography to carry out molecular weight determination to the Radix Codonopsis polysaccharide that adopts extracting method of the present invention to obtain: efficient gel permeation chromatography system: pump: waters 515 HPLC pump; Work station: Millennium
32Detector: waters 2410RID; Pillar: TSK GMPWXL; Detector temperature: 35 ℃; Column temperature: 35 ℃; Flow velocity: 0.3ml/min; Mobile phase: ultra-pure water; Radix Codonopsis polysaccharide CPS molecular weight becomes multiplet to distribute between 2500～3,000,000 Dal as a result.
The present invention can adopt following method that the above-mentioned Radix Codonopsis polysaccharide extract that obtains is carried out further purification: the Radix Codonopsis polysaccharide water for preparing is fully dissolved, centrifugal, weak anionic exchange column on the supernatant, flow velocity 1ml/min, earlier through the distilled water eluting, the phenolsulfuric acid method is followed the tracks of and is detected, and till no positive eluent, merges the eluent that the polysaccharide reaction is positive; Reuse NaCl solution is successively with the Concentraton gradient eluting of 0.01mol/L, 0.05mol/L, 0.1mol/L, 0.15mol/L, 0.2mol/L, 0.25mol/L, 0.3mol/L, 0.5mol/L, 1.0mol/L, the phenolsulfuric acid method is followed the tracks of and is detected, till no positive eluent, merge the eluent that the polysaccharide reaction is positive.Distilled water eluting part obtains the Radix Codonopsis polysaccharide subfraction through concentrated, vacuum drying; Each NaCl eluant solution part of variable concentrations obtains each subfraction of Radix Codonopsis polysaccharide through concentrating under reduced pressure, dialysis, vacuum drying.Utilization efficient gel permeation chromatography is measured the molecular weight of each subfraction of Radix Codonopsis polysaccharide, and measurement result is: each subfraction of Radix Codonopsis polysaccharide distributes between 2500～3,000,000 Dal.
Also can adopt following method that the above-mentioned Radix Codonopsis polysaccharide extract that obtains is carried out further purification: to extract the Radix Codonopsis polysaccharide that obtains, water fully dissolves, centrifugal, weak anionic exchange column chromatographic isolation and purification on the supernatant, flow velocity 1ml/min, earlier through the distilled water eluting, the phenolsulfuric acid method is followed the tracks of and is detected, till no positive eluent, merge the eluent that the polysaccharide reaction is positive; Reuse NaHCO
3Solution is successively with Concentraton gradient eluting and the 0.05mol/LNaOH eluant solution of 0.01mol/L, 0.05mol/L, 0.1mol/L, 0.15mol/L, 0.2mol/L, 0.25mol/L, 0.3mol/L, 0.5mol/L, 1.0mol/L, the phenolsulfuric acid method is followed the tracks of and is detected, till no positive eluent, merge the eluent that the polysaccharide reaction is positive; Distilled water eluting part gets the Radix Codonopsis polysaccharide subfraction through concentrated, vacuum drying; Variable concentrations NaHCO
3The eluant solution part obtains each subfraction of Radix Codonopsis polysaccharide through concentrating under reduced pressure, dialysis, vacuum drying.Utilization efficient gel permeation chromatography is measured the molecular weight of each subfraction of Radix Codonopsis polysaccharide, and measurement result is: each subfraction of Radix Codonopsis polysaccharide distributes between 2500～3,000,000 Dal.
Is 1000～200 with the exclusion scope with each subfraction of Radix Codonopsis polysaccharide of obtaining respectively after with water dissolution, and the molecular sieve column chromatography of 000Dal separates, obtain a series of molecular weight different but each series is respectively each subfraction of highly purified Radix Codonopsis polysaccharide of homogeneous molecular weight.Utilization efficient gel permeation chromatography is measured the molecular weight of each subfraction of Radix Codonopsis polysaccharide, and measurement result is: each subfraction of Radix Codonopsis polysaccharide distributes between 2500～3,000,000 Dal.
The extracting method of Radix Codonopsis polysaccharide of the present invention belongs to decoction and alcohol sedimentation technique.On the basis of a large amount of experiments, the Radix Codonopsis polysaccharide extraction process technology parameter that the present invention has determined to optimize (comprising the relative density, alcohol precipitation concentration of solvent that remove impurity is used and concentration, extracting solution etc.), improved the content (reaching more than 70%) of Radix Codonopsis polysaccharide, extraction rate reached to 20%～30%; The Radix Codonopsis polysaccharide impurity that this extracting method step is easy, with low cost, process stabilizing and extraction obtain is few, appearance character is good.Extracting method of the present invention has increased the subsequent purification processing, not only can well Radix Codonopsis polysaccharide be separated by acidic polysaccharose and neutral polysaccharide after being further purified, and Radix Codonopsis polysaccharide can be carried out segmentation by the molecular weight size, thereby obtain different Radix Codonopsis polysaccharide subfractions, simultaneously, by molecular weight determination to each subfraction of Radix Codonopsis polysaccharide, the further clear and definite formation component of Radix Codonopsis polysaccharide.The Radix Codonopsis polysaccharide that adopts extracting method of the present invention to obtain can be applied in preparation antineoplastic agent, the medicine for treating thrombus of invigorating blood circulation, hypotensor, hypolipidemic, blood sugar lowering, defying age medicine, enhancing bone marrow hematogenesis function medicine or immunoregulation medicament.
The specific embodiment
Embodiment 1
The extracting method of Radix Codonopsis polysaccharide, comprise the steps: that (1) dedusting is clean: medical material Radix Codonopsis 100g flushing with clean water is clean; (2) chopping: the section that Radix Codonopsis is cut into 2～4cm; (3) remove impurity: with the Radix Codonopsis section that cuts, 80% alcohol reflux 2 times each 1 hour, volatilizes ethanol; (4) water is carried: the medicinal residues after the remove impurity add the distilled water of 8 times of medicinal residues weight, and boiling water refluxes 2 times, and each 1 hour, filter, merging filtrate, being evaporated to relative density of medicine liquid is 0.8g/ml, places cooling; (5) ethanol precipitation: will place refrigerative concentrated solution adding ethanol to alcohol precipitation concentration is 70%, leaves standstill, centrifugal, gets pure hypostasis; (6) dehydration: pure hypostasis is washed successively with dehydrated alcohol, acetone, ether; (7) drying: the pure hypostasis vacuum drying after will washing, getting the yellow-white powdery precipitate is the 35.5g of Radix Codonopsis polysaccharide extract.Measure the extract total sugar content with the phenolsulfuric acid method, 3,5-dinitrosalicylic acid method is measured the extract contents of monosaccharides, and total sugar content deducts contents of monosaccharides and is Radix Codonopsis polysaccharide content.Radix Codonopsis polysaccharide content is 76% with glucose meter.It is 27% that extraction ratio is calculated as follows.
The Radix Codonopsis polysaccharide water that extraction is obtained fully dissolves, and is centrifugal, weak anionic exchange column on the supernatant, and flow velocity 1ml/min, earlier through the distilled water eluting, the phenolsulfuric acid method is followed the tracks of and is detected, and till no positive eluent, merges the eluent that the polysaccharide reaction is positive; Reuse NaCl solution is successively with the Concentraton gradient eluting of 0.01mol/L, 0.05mol/L, 0.1mol/L, 0.15mol/L, 0.2mol/L, 0.25mol/L, 0.3mol/L, 0.5mol/L, 1.0mol/L, the phenolsulfuric acid method is followed the tracks of and is detected, till no positive eluent, merge the eluent that the polysaccharide reaction is positive.Distilled water eluting part through concentrate,, vacuum drying gets the Radix Codonopsis polysaccharide subfraction; Each NaCl eluant solution part of variable concentrations obtains each subfraction of Radix Codonopsis polysaccharide through concentrating under reduced pressure, dialysis, vacuum drying.Described weak anionic exchange column is a DEAE-weak anionic exchange column.And preferred DEAE-Sepharose F.F. post or DEAE cellulose column or DEAE-Sephdex A.
Is 1000～200 with the exclusion scope with the Radix Codonopsis polysaccharide subfraction that obtains respectively after with water dissolution, and the molecular sieve column chromatography of 000Dal separates, obtain a series of molecular weight different but each series is respectively each subfraction of highly purified Radix Codonopsis polysaccharide of homogeneous molecular weight.Utilization efficient gel permeation chromatography is measured the molecular weight of each subfraction of Radix Codonopsis polysaccharide, and measurement result is: each subfraction of Radix Codonopsis polysaccharide distributes between 2500～3,000,000 Dal.All contain monosaccharide residues such as D-glucose, L-rhamnose, D-galactose, D-arabinose, glucuronic acid, galacturonic acid.Wherein molecular sieve column is Sephdex G or Sephacryl gel column or Sephrose 6B gel column.
Embodiment 2
The extracting method of Radix Codonopsis polysaccharide, comprise the steps: that (1) dedusting is clean: medical material Radix Codonopsis 100g flushing with clean water is clean; (2) chopping: the section that Radix Codonopsis is cut into 2～4cm; (3) remove impurity: with the Radix Codonopsis section that cuts, 95% alcohol reflux 3 times each 2 hours, volatilizes ethanol; (4) water is carried: the medicinal residues after the remove impurity add the distilled water of 20 times of medicinal residues weight, and boiling water refluxes 2 times, and each 2 hours, filter, merging filtrate, being evaporated to relative density of medicine liquid is 1.2g/ml, places cooling; (5) ethanol precipitation: will place refrigerative concentrated solution adding ethanol to alcohol precipitation concentration is 90%, leaves standstill, centrifugal, gets pure hypostasis; (6) dehydration: pure hypostasis is washed successively with dehydrated alcohol, acetone, ether; (7) drying: the pure hypostasis vacuum drying after will washing, getting the yellow-white powdery precipitate is the 33g of Radix Codonopsis polysaccharide extract.Measure the extract total sugar content with the phenolsulfuric acid method, 3,5-dinitrosalicylic acid method is measured the extract contents of monosaccharides, and total sugar content deducts contents of monosaccharides and is Radix Codonopsis polysaccharide content.Radix Codonopsis polysaccharide content is 80% with glucose meter.It is 26% that extraction ratio is calculated as follows.
The Radix Codonopsis polysaccharide water that extraction is obtained fully dissolves, centrifugal, weak anionic exchange column chromatographic isolation and purification on the supernatant, flow velocity 1ml/min, the weak anionic exchange column is earlier through the distilled water eluting, the phenolsulfuric acid method is followed the tracks of and is detected, and till no positive eluent, merges the eluent that the polysaccharide reaction is positive; Reuse NaHCO
3Solution is successively with Concentraton gradient eluting and the 0.05mol/LNaOH eluant solution of 0.01mol/L, 0.05mol/L, 0.1mol/L, 0.15mol/L, 0.2mol/L, 0.25mol/L, 0.3mol/L, 0.5mol/L, 1.0mol/L, the phenolsulfuric acid method is followed the tracks of and is detected, till no positive eluent, merge the eluent that the polysaccharide reaction is positive; Distilled water eluting part gets the Radix Codonopsis polysaccharide subfraction through concentrated, vacuum drying; Variable concentrations NaHCO
3The eluant solution part obtains each subfraction of Radix Codonopsis polysaccharide through concentrating under reduced pressure, dialysis, vacuum drying.Described weak anionic exchange column is a DEAE-weak anionic exchange column.And preferred DEAE-Sepharose F.F. post or DEAE cellulose column or DEAE-Sephdex A.
Is 1000～200 with the exclusion scope with the Radix Codonopsis polysaccharide subfraction that obtains respectively after with water dissolution, and the molecular sieve column chromatography of 000Dal separates, obtain a series of molecular weight different but each series is respectively each subfraction of highly purified Radix Codonopsis polysaccharide of homogeneous molecular weight.Utilization efficient gel permeation chromatography is measured the molecular weight of each subfraction of Radix Codonopsis polysaccharide, and measurement result is: each subfraction of Radix Codonopsis polysaccharide distributes between 2500～3,000,000 Dal.All contain monosaccharide residues such as D-glucose, L-rhamnose, D-fructose, D-galactose, D-arabinose, D-fructose, D-mannose.Wherein molecular sieve chromatography is Sephdex G or Sephacryl gel column or Sephrose 6B gel column.
Embodiment 3
The extracting method of Radix Codonopsis polysaccharide, comprise the steps: that (1) dedusting is clean: medical material Radix Codonopsis 300g flushing with clean water is clean; (2) chopping: the section that Radix Codonopsis is cut into 2～4cm; (3) remove impurity: with the Radix Codonopsis section that cuts, 90% (or 85% ethanol) alcohol reflux 3 times each 1.5 hours, volatilizes ethanol; (4) water is carried: the medicinal residues after the remove impurity add the distilled water of 15 times of (or 10 times or 13 times or 18 times) medicinal residues weight, boiling water reflux, extract, 3 times, and each 1.5 hours, filter, merging filtrate, being evaporated to relative density of medicine liquid is 1.0g/ml, places cooling; (5) ethanol precipitation: will place refrigerative concentrated solution adding ethanol to alcohol precipitation concentration is 80% (or 75% or 85%), leaves standstill, centrifugal, gets pure hypostasis; (6) dehydration: pure hypostasis is washed successively with dehydrated alcohol, acetone, ether; (7) drying: the pure hypostasis vacuum drying after will washing, getting the yellow-white powdery precipitate is the 111g of Radix Codonopsis polysaccharide extract.Measure the extract total sugar content with the phenolsulfuric acid method, 3,5-dinitrosalicylic acid method is measured the extract contents of monosaccharides, and total sugar content deducts contents of monosaccharides and is Radix Codonopsis polysaccharide content.Radix Codonopsis polysaccharide content is 77.8% with glucose meter.It is 28.8% that extraction ratio is calculated as follows.
The Radix Codonopsis polysaccharide water that extraction is obtained fully dissolves, and is centrifugal, weak anionic exchange column on the supernatant, and flow velocity 1ml/min, earlier through the distilled water eluting, the phenolsulfuric acid method is followed the tracks of and is detected, and till no positive eluent, merges the eluent that the polysaccharide reaction is positive; Reuse NaCl solution is successively with the Concentraton gradient eluting of 0.01mol/L, 0.05mol/L, 0.1mol/L, 0.15mol/L, 0.2mol/L, 0.25mol/L, 0.3mol/L, 0.5mol/L, 1.0mol/L, the phenolsulfuric acid method is followed the tracks of and is detected, till no positive eluent, merge the eluent that the polysaccharide reaction is positive.Distilled water eluting part gets the Radix Codonopsis polysaccharide subfraction through concentrated, vacuum drying; Each NaCl eluant solution part of variable concentrations obtains each subfraction of Radix Codonopsis polysaccharide through concentrating under reduced pressure, dialysis, vacuum drying.Described weak anionic exchange column is a DEAE-weak anionic exchange column.And preferred DEAE-Sepharose F.F. post or DEAE cellulose column or DEAE-Sephdex A.
Is 1000～200 with the exclusion scope with the Radix Codonopsis polysaccharide subfraction that obtains respectively after with water dissolution, and the molecular sieve column chromatography of 000Dal separates, obtain a series of molecular weight different but each series is respectively each subfraction of highly purified Radix Codonopsis polysaccharide of homogeneous molecular weight.Utilization efficient gel permeation chromatography is measured the molecular weight of each subfraction of Radix Codonopsis polysaccharide, and measurement result is: each subfraction of Radix Codonopsis polysaccharide distributes between 2500～3,000,000 Dal.All contain monosaccharide residues such as D-glucose, L-rhamnose, D-galactose, D-arabinose, glucuronic acid, galacturonic acid.Wherein molecular sieve column is Sephdex G or Sephacryl gel column or Sephrose 6B gel column.
Claims (7)
1. the extracting method of a Radix Codonopsis polysaccharide is characterized by: comprise the steps: that (1) dedusting cleans: medical material Radix Codonopsis flushing with clean water is clean; (2) chopping: the section that Radix Codonopsis is cut into 2～4cm; (3) remove impurity: with the Radix Codonopsis section that cuts, 80%～95% alcohol reflux 2～3 times each 1～2 hour, volatilizes ethanol; (4) water is carried: the medicinal residues after the remove impurity add the distilled water of 8～20 times of medicinal residues weight, and boiling water refluxes 2～3 times, and each 1～2 hour, filter, merging filtrate, being evaporated to relative density of medicine liquid is 0.8～1.2g/ml, places cooling; (5) ethanol precipitation: will place refrigerative concentrated solution adding ethanol to alcohol precipitation concentration is 70%～90%, leaves standstill, centrifugal, obtains pure hypostasis; (6) dehydration: pure hypostasis is washed successively with dehydrated alcohol, acetone, ether; (7) drying: the pure hypostasis vacuum drying after will washing, getting the yellow-white powdery precipitate is Radix Codonopsis polysaccharide extract.
2. the extracting method of Radix Codonopsis polysaccharide as claimed in claim 1, it is characterized by: the Radix Codonopsis polysaccharide water for preparing is fully dissolved, centrifugal, weak anionic exchange column on the supernatant, flow velocity 1ml/min, weak anionic exchange column are earlier through the distilled water eluting, and the phenolsulfuric acid method is followed the tracks of and detected, till no positive eluent, merge the eluent that the polysaccharide reaction is positive; Reuse NaCl solution is successively with the Concentraton gradient eluting of 0.01mol/L, 0.05mol/L, 0.1mol/L, 0.15mol/L, 0.2mol/L, 0.25mol/L, 0.3mol/L, 0.5mol/L, 1.0mol/L, the phenolsulfuric acid method is followed the tracks of and is detected, till no positive eluent, merge the eluent that the polysaccharide reaction is positive; Distilled water eluting part gets the Radix Codonopsis polysaccharide subfraction through concentrated, vacuum drying; Each NaCl eluant solution part of variable concentrations gets each subfraction of Radix Codonopsis polysaccharide through concentrating under reduced pressure, dialysis, vacuum drying.
3. the extracting method of Radix Codonopsis polysaccharide as claimed in claim 1, it is characterized by: the Radix Codonopsis polysaccharide water that extraction is obtained fully dissolves, centrifugal, weak anionic exchange column column chromatographic isolation and purification on the supernatant, flow velocity 1ml/min, weak anionic exchange column are earlier through the distilled water eluting, and the phenolsulfuric acid method is followed the tracks of and detected, till no positive eluent, merge the eluent that the polysaccharide reaction is positive; Reuse NaHCO
3Solution is successively with Concentraton gradient eluting and the 0.05mol/L NaOH eluant solution of 0.01mol/L, 0.05mol/L, 0.1mol/L, 0.15mol/L, 0.2mol/L, 0.25mol/L, 0.3mol/L, 0.5mol/L, 1.0mol/L, the phenolsulfuric acid method is followed the tracks of and is detected, till no positive eluent, merge the eluent that the polysaccharide reaction is positive; Distilled water eluting part gets the Radix Codonopsis polysaccharide subfraction through concentrated, vacuum drying; Each NaHCO of variable concentrations
3The eluant solution part gets each subfraction of Radix Codonopsis polysaccharide through concentrating under reduced pressure, dialysis, vacuum drying.
4. as the extracting method of claim 2 or 3 described Radix Codonopsis polysaccharides, it is characterized by: is 1000～200 with the exclusion scope with each subfraction of Radix Codonopsis polysaccharide of obtaining respectively after with water dissolution, the molecular sieve column chromatography of 000Dal separates, obtain a series of molecular weight different but each series is respectively each subfraction of highly purified Radix Codonopsis polysaccharide of homogeneous molecular weight.
5. the extracting method of claim 2 or 3 described Radix Codonopsis polysaccharides, it is characterized by: the weak anionic exchange column is a DEAE-weak anionic exchange column.
6. the extracting method of Radix Codonopsis polysaccharide as claimed in claim 5 is characterized by: DEAE-weak anionic exchange column preferred DEAE-sepharose F.F. post or DEAE cellulose column or DEAE-Sephdex A.
7. the extracting method of Radix Codonopsis polysaccharide as claimed in claim 4, it is characterized by: molecular sieve column is Sephadex G or Sephacryl gel column or Sepharose 6B gel column.
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