CN106621810A - Oligochitosan separation and purification apparatus and method therefor - Google Patents
Oligochitosan separation and purification apparatus and method therefor Download PDFInfo
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- CN106621810A CN106621810A CN201710148201.XA CN201710148201A CN106621810A CN 106621810 A CN106621810 A CN 106621810A CN 201710148201 A CN201710148201 A CN 201710148201A CN 106621810 A CN106621810 A CN 106621810A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D57/00—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C
- B01D57/02—Separation, other than separation of solids, not fully covered by a single other group or subclass, e.g. B03C by electrophoresis
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/02—Reverse osmosis; Hyperfiltration ; Nanofiltration
- B01D61/027—Nanofiltration
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Abstract
The invention relates to an oligochitosan separation and purification apparatus and a method therefor. The apparatus comprises a separation and purification chamber, a negative pole, a positive pole, and at least one nano-filtering membrane. The separation and purification chamber is used for accommodating an oligochitosan solution; the negative pole is arranged in the separation and purification chamber; the positive pole is disposed in a zone, which is separated from the negative pole, inside the separation and purification chamber, so that an electrophoresis zone is formed between the positive pole and the negative pole; the nano-filtering membrane is disposed in the electrophoresis zone so as to separate the electrophoresis zone into at least two sub electrophoresis zones. The apparatus has simple structure and reasonable design, and is high in yield during separation and purification of an oligochitosan sample. The invention also provides a method for separation and purification of the oligochitosan. The method includes a step of separation and purification through multi-stage membranes. The method is simple in processes, has short production period and high product yield, can separate and purify the oligochitosan sample in large scale and can achieve large-scale production.
Description
Technical field
The present invention relates to technical field of biochemical industry, more particularly to a kind of oligochitosan isolates and purifies devices and methods therefor.
Background technology
Oligochitosan is called Chitosan oligosaccharide, oligo-chitosan, and it is bonded with β-Isosorbide-5-Nitrae-glucosides to refer to 2-10 glucosamine
Oligosaccharide, it be typically by the product-shitosan of de-acetyl chitin again through physics, chemistry, the side of biology
What method was degraded and was obtained, therefore yield is huge.Oligochitosan molecular weight≤3200Da, is that water solublity is preferable, function is big, biological
The high low molecular weight product of activity.It has the unexistent higher solubility of shitosan, and water is dissolved in entirely, is easily absorbed by organism
Many unique functions such as utilize.
Oligochitosan is unique positively charged cation basic amine group oligosaccharide, immunity of the oligochitosan to human body in nature
Regulation, antitumor, blood fat reducing, adjust blood glucose, improve liver and cardio-pulmonary function and other different physiological roles important roles.
The oligochitosan of different molecular weight its function is had nothing in common with each other, such as：The substantial amounts of sugar of experiment display 5,6 sugar have good suppression tumor
Function, therefore the oligochitosan of purification, can play more effectively effect to the production of human society, life.But, pass through at present
The oligochitosan that various technique productions go out all is that the mixture of 2-10 sugar even also has some monosaccharide and small molecule polysaccharide to mix wherein,
And existing oligochitosan purification technique and technique can only small lot prepare the oligochitosan of higher degree, it is difficult to satisfy social needs,
And price is high.
The content of the invention
To solve problem above, the present invention provides the devices and methods therefor that can in high volume isolate and purify oligochitosan.
The embodiment of the present invention provides a kind of oligochitosan and isolates and purifies device, including isolating and purifying room, negative electrode, anode and at least
One NF membrane, the room that isolates and purifies is to putting part oligosaccharide solution；The negative electrode isolates and purifies interior described in being arranged at；Institute
State anode be arranged at it is described isolate and purify interior and arrange with cathode separation, surround Zone electophoresis between the anode, negative electrode；It is described
At least one NF membrane is arranged in the Zone electophoresis so that the Zone electophoresis are separated into at least two sub- Zone electophoresis, works as NF membrane
When quantity is 2 or more, each nanofiltration membrane aperture is successively decreased from anode to cathode direction.
In present pre-ferred embodiments, the oligochitosan isolates and purifies device also includes counterflow-preventing baffle plate, the counterflow-preventing
Located at each NF membrane near the side of negative electrode, the counterflow-preventing baffle plate is 0.5～5cm apart from the distance of NF membrane to baffle plate.
In present pre-ferred embodiments, the oligochitosan isolates and purifies device also to be included arranging to test in sub- Zone electophoresis
The conductivity meter of electrical conductivity of solution value.
In present pre-ferred embodiments, the oligochitosan isolates and purifies device also to be included for taking that oligochitosan solution flows out
Liquid valve.
The embodiment of the present invention also provides a kind of oligochitosan isolation and purification method, comprises the following steps：
Oligochitosan solution is injected into into oligochitosan to isolate and purify in device, energization purification, wherein oligochitosan separation is pure
Makeup is put including isolating and purifying room, negative electrode, anode and at least one NF membrane, and described to isolate and purify room molten to house oligochitosan
Liquid；The negative electrode isolates and purifies interior described in being arranged at；The anode isolates and purifies indoor and and cathode separation described in being arranged at
Arrange, between the anode, negative electrode Zone electophoresis are surrounded；At least one NF membrane is arranged in the Zone electophoresis with will be described
Zone electophoresis are separated at least two sub- Zone electophoresis, and when NF membrane quantity is 2 or more, each nanofiltration membrane aperture is from anode to the moon
Extreme direction is successively decreased.
In present pre-ferred embodiments, the purification oligochitosan solution that step (2) is obtained is carried out into two as oligochitosan sample
Secondary purification, and detached different molecular weight magnitude range selects the nanofiltration of different pore size according to required for purification oligochitosan solution
Film, is further purified.
In present pre-ferred embodiments, drying meanss described in step (3) include being spray-dried and lyophilization.
In present pre-ferred embodiments, multistage membrane separating and purifying process includes in step (2)：
(21) fluid injection：Deionized water is injected into Zone electophoresis, the oligochitosan sample injection that step (1) is prepared is near sun
In the sub- Zone electophoresis of pole；
(22) it is powered：Treat that oligochitosan mother solution is mixed homogeneously with deionized water in sub- Zone electophoresis, when solution concentration reach it is predetermined
During value, it is powered, the electric field intensity value is 20～80V/cm；
(23) power-off：Each sub- Zone electophoresis internal conductance rate instrument reading change is observed, when the electricity of the sub- Zone electophoresis closest to negative electrode
After electrical conductivity instrument reading tends towards stability, power supply is closed；
(24) liquid is taken：Collect purification oligochitosan solution in each sub- Zone electophoresis.
In present pre-ferred embodiments, the oligochitosan solution concentration is 0.3-15%.
Beneficial effects of the present invention are as follows：
1st, oligochitosan of the invention isolates and purifies device, and simple structure is reasonable in design, isolates and purifies oligochitosan sample yield
It is high；
2nd, oligochitosan isolation and purification method of the invention, safe operation process, simple, product yield height, can in high volume divide
From purification oligochitosan sample, it is easy to the realization of large-scale production；
3rd, oligochitosan isolation and purification method of the invention saves the use of organic reagent, reduces the row of discarded organic reagent
Put, reduce environmental pollution.
Description of the drawings
Below in conjunction with the accompanying drawings, described in detail by the specific embodiment to the present invention, technical scheme will be made
And other beneficial effects are apparent.
Fig. 1 isolates and purifies apparatus structure schematic diagram for the oligochitosan of the embodiment of the present invention；
Fig. 2 isolates and purifies the sectional view of device for the oligochitosan of the embodiment of the present invention.
Main element symbol description：
100th, device is isolated and purified；10th, room is isolated and purified；11st, sub- Zone electophoresis；20a, anode；20b, negative electrode；30th, nanofiltration
Film；40th, conductivity meter；50th, liquid taking valve.
Specific embodiment
Further to illustrate the technological means taken of the invention and its effect, below in conjunction with being embodied as the present invention
Example and its accompanying drawing are described in detail.
The embodiment of the present invention provides a kind of oligochitosan and isolates and purifies device 100 (referring to Fig. 1 and Fig. 2), including isolating and purifying
Room 10, negative electrode 20b, anode 20a and at least one NF membrane 30, the room 10 that isolates and purifies is to putting part oligosaccharide solution；Institute
State negative electrode 20b and be arranged at described one end isolated and purified in room 10；The anode 20a is arranged at described isolating and purifying in room 10
The other end；The anode 20a and negative electrode 20b interval settings, between the anode 20a and negative electrode 20b Zone electophoresis are surrounded；It is described to receive
Filter membrane 30 is arranged in the Zone electophoresis so that the Zone electophoresis are separated into at least two sub- Zone electophoresis 11, when the quantity of NF membrane 30
For 2 or more when, the aperture of each NF membrane 30 is successively decreased from anode 20a to negative electrode 20b directions.
In the embodiment of the present invention, from anode 20a to negative electrode 20b directions at least provided with a NF membrane 30, so due to shell
Oligosaccharide positively charged, therefore after powered up oligochitosan will be moved to negative electrode 20b, simultaneously because have the presence of NF membrane 30, therefore point
Son amount will be through NF membrane 30 less than the oligochitosan of the aperture specification of NF membrane 30, and molecular weight is larger will be trapped, thus
The oligochitosan of different molecular weight is distinguished.
NF membrane be it is a kind of permission solvent molecule or some low molecular weight solutes or low price ion permeable feature it is semi-transparent
Film.It gains the name because the size that can retain material is about nanometer, aperture in more than 1nm, general 1-2nm.NF membrane can be directed to
The object for being filtered has different specifications.
Specifically, the inwall for isolating and purifying room 10 is provided with longitudinally disposed draw-in groove bar, and the clamping of the NF membrane 30 enters
In the draw-in groove bar.Alternatively, the inwall of NF membrane 30 and purification room 10 can also be integrally formed.
When there is multiple NF membrane 30, the NF membrane 30 from anode 20a to negative electrode 20b directions interval setting,
Zone electophoresis are separated into into many sub- Zone electophoresis 11.For example there are 5 NF membrane, then Zone electophoresis are distinguished into 6 sub- Zone electophoresis.There is N
Individual NF membrane, then Zone electophoresis be distinguished into N+1 sub- Zone electophoresis.
The oligochitosan isolates and purifies device 100 also includes counterflow-preventing baffle plate, and the counterflow-preventing baffle plate is located at each NF membrane
30 is 0.5～5cm apart from the distance of NF membrane 30 near the side of negative electrode 20b, the counterflow-preventing baffle plate.Counterflow-preventing baffle plate is prevented
Electrophoresis liquid circulates between adjacent two sub- Zone electophoresis 11, is so easy to individually take out the oligochitosan solution (purification in each sub- Zone electophoresis
Liquid).
The oligochitosan isolates and purifies device 100 also to be included testing the conductivity meter of electrophoresis liquid in sub- Zone electophoresis 11
40, real-time monitoring electrical conductivity can in time understand the progress of purification, and for example, electrical conductivity no longer changes, and show to there is no longer oligochitosan
There is apparent motion in ion.
The oligochitosan isolates and purifies the liquid taking valve 50 that device 100 also includes being flowed out to oligochitosan solution.Preferably, institute
State liquid taking valve 50 and be arranged at intervals at the bottom for isolating and purifying device 100.At least one liquid taking valve is set in per individual sub- Zone electophoresis 11
50。
The embodiment of the present invention provides a kind of oligochitosan isolation and purification method, and the purification process is isolated and purified using the present invention's
Device 100 is comprised the following steps carrying out：
(1) standby liquid：The oligochitosan sample of purification to be separated is dissolved in deionized water, precipitation, filtration form oligochitosan
Mother solution；
(2) multistage membrane separation purification：Oligochitosan mother solution is injected into into oligochitosan to isolate and purify in device 100, and be powered into
Row electrophoresis.
Specifically, multistage membrane separating and purifying process includes in step (2)：
(21) fluid injection：Deionized water is injected into Zone electophoresis, the oligochitosan sample injection that step (1) is prepared is near sun
In the first sub- Zone electophoresis 11 of pole 20a；
(22) it is powered：Treat that oligochitosan mother solution is mixed homogeneously with deionized water in the first sub- Zone electophoresis 11, work as electrophoretic liquid concentration
When reaching predetermined value, it is powered, the electric field intensity value is 20～80V/cm；
Specifically, the oligochitosan solution concentration predetermined value is 0.3～15%, is herein w/v, is g/
100mL。
(23) each reading of 11 internal conductance rate instrument of sub- Zone electophoresis 40 change is observed, when the sub- Zone electophoresis 11 closest to negative electrode 20b
The reading of conductivity meter 40 tend towards stability and continue at least 3 minutes after, close power supply；
(24) liquid is taken：The liquid taking valve 50 in the sub- Zone electophoresis 11 of target is pulled out, purification oligochitosan in each sub- Zone electophoresis 11 is collected
Solution.
Used as the preferred embodiment of the present invention, the purification oligochitosan solution that can be obtained step (2) is used as oligochitosan sample
Product carry out secondarily purified, that is, repeat the above steps (22)-(24), and according to required for purification oligochitosan solution it is detached not
The aperture of NF membrane 30 is selected with molecular size range scope, is further purified.
(3) it is dried (optional)：Purification oligochitosan solution is taken out by the liquid taking valve 50 of each sub- Zone electophoresis 11 to be dried, obtain
Obtain purification oligochitosan product.The drying meanss include being spray-dried and lyophilization.
It is below the oligochitosan degree of polymerization and molecular weight synopsis.
The oligochitosan of table 1. is polymerized and molecular weight synopsis
It is below specific embodiment, the specification of NF membrane 30 of employing is divided into 500 types, 800 types, 1000 types and 1500 types, point
Do not retain the oligochitosan of more than 500 dalton, 800 dalton, 1000 dalton and 1500 Dalton molecular weights (containing this number).
Embodiment 1
With reference to Fig. 1 and Fig. 2,4 NF membrane 30 are set isolating and purifying in room 10, the clamping of the NF membrane 30 enters to isolate and purify
On the inboard wall groove of room 10, room 10 will be isolated and purified and is separated into 5 sub- Zone electophoresis, the NF membrane 30 be respectively 1500 types, 1000
Type, 800 types, 500 types, wherein 1500 types are near anode side, 500 types are near negative electrode side.By the oligochitosan of purification to be separated
Sample is dissolved in deionized water, precipitation, filtration, forms 30% oligochitosan mother solution；In the sub- Zone electophoresis 11 near anode 20a
The above-mentioned oligochitosan mother solution of interior addition 100ml, is subsequently adding 9.9L deionized waters, it is to be mixed it is uniform after, oligochitosan electrophoretic liquid concentration
For 0.3%.Its minor Zone electophoresis is separately added into 10L deionized waters.It is powered, the electric field intensity of energization is 20V/cm.Treat close
The electrical conductivity of the sub- Zone electophoresis 11 of negative electrode 20b tend towards stability and it is continual and steady 3 minutes after, take out from the liquid taking valve 50 of each Zone electophoresis
Purification oligochitosan solution, through being spray-dried oligochitosan product is obtained, through HPLC detections, from anode 20a to negative electrode 20b side
It is respectively to, the oligochitosan product degree of polymerization type of each sub- Zone electophoresis 11 and yield：10-9 sugar 94%, 8-7 sugar 95%, 6-5 is sugared
94%, 4-3 sugar 92%, 2 sugar 96%.
Embodiment 2
4 sub- Zone electophoresis are set using 3 NF membrane in the present embodiment.The oligochitosan sample of purification to be separated is dissolved in
In deionized water, precipitation, filtration form the oligochitosan mother solution of 1g/ml；Add in the sub- Zone electophoresis 11 near anode 20a
Mother solution is stated, deionized water is subsequently adding so that after mix homogeneously, oligochitosan electrophoretic liquid concentration is 0.5%, overall solution volume is
10L.Deionized water is added in its minor Zone electophoresis, 10L is respectively added.It is powered, the electric field intensity of energization is 40V/cm.It is pure separating
Change in room 10 and set 3 NF membrane 30, the clamping of the NF membrane 30 enters to isolate and purify on the inboard wall groove of room 10, described from anode 20a
Aperture to the NF membrane 30 in negative electrode 20b directions is 1000 types, 800 types, 500 types, treats the sub- Zone electophoresis 11 near negative electrode 20b
Electrical conductivity tend towards stability after, purification oligochitosan solution is taken out from the liquid taking valve 50 of each Zone electophoresis, through HPLC detections, from anode
20a to the oligochitosan product degree of polymerization type and yield of each sub- Zone electophoresis 11 in negative electrode 20b directions are respectively：10-7 sugar 93%,
6-5 sugar 94%, 4-3 sugar 92%, 2 sugar 96%.
Embodiment 3
4 sub- Zone electophoresis are set using 3 NF membrane in the present embodiment.The oligochitosan sample of purification to be separated is dissolved in
In deionized water, precipitation, filtration form the oligochitosan mother solution of 2g/ml；Add in the sub- Zone electophoresis 11 near anode 20a
Mother solution is stated, deionized water is subsequently adding so that after mix homogeneously, oligochitosan electrophoretic liquid concentration is 1.25%, liquor capacity is
10L.Deionized water is added in its minor Zone electophoresis, 10L is respectively added.It is powered, the electric field intensity of energization is 50V/cm.It is pure separating
Change in room 10 and set 3 NF membrane 30, the clamping of the NF membrane 30 enters to isolate and purify on the inboard wall groove of room 10, described from anode 20a
Aperture to each NF membrane 30 in negative electrode 20b directions is 1500 types, 800 types, 500 types, treats the sub- Zone electophoresis near negative electrode 20b
After 11 electrical conductivity tends towards stability, purification oligochitosan solution is taken out from the liquid taking valve 50 of each Zone electophoresis, through HPLC detections, from sun
Pole 20a to the oligochitosan product degree of polymerization type and yield of each sub- Zone electophoresis 11 in negative electrode 20b directions are respectively：10-9 is sugared
94%, 8-5 sugar 94%, 4-3 sugar 92%, 2 sugar 96%.
Embodiment 4
4 sub- Zone electophoresis are set using 3 NF membrane in the present embodiment.The oligochitosan sample of purification to be separated is dissolved in
In deionized water, precipitation, filtration form the oligochitosan mother solution of 3.4g/ml；Add in the sub- Zone electophoresis 11 near anode 20a
Above-mentioned mother solution, is subsequently adding deionized water so that it is to be mixed it is uniform after, oligochitosan electrophoretic liquid concentration is 2.72%, liquor capacity
For 10L.Deionized water is added in its minor Zone electophoresis, 10L is respectively added.It is powered, the electric field intensity of energization is 60V/cm.Separating
3 NF membrane 30 are set in purification room 10, the clamping of the NF membrane 30 enters to isolate and purify on the inboard wall groove of room 10, the NF membrane 30
Aperture be 1500 types, 1000 types, 800 types, after the electrical conductivity of the sub- Zone electophoresis 11 near negative electrode 20b tends towards stability, from each
The liquid taking valve 50 of Zone electophoresis takes out purification oligochitosan solution, and through being spray-dried oligochitosan product is obtained, and detects through HPLC, from
Anode 20a to the oligochitosan product degree of polymerization type and yield of each sub- Zone electophoresis 11 in negative electrode 20b directions are respectively：10-9 is sugared
94%, 8-7 sugar 95%, 6-5 sugar 94%, 4-2 sugar 96%.
Embodiment 5
3 sub- Zone electophoresis are set using 2 NF membrane in the present embodiment.The oligochitosan sample of purification to be separated is dissolved in
In deionized water, precipitation, filtration form the oligochitosan mother solution of 6g/ml；Add in the sub- Zone electophoresis 11 near anode 20a
Mother solution is stated, deionized water is subsequently adding so that after mix homogeneously, oligochitosan electrophoretic liquid concentration is 15%, and liquor capacity is 10L.
Deionized water is added in remaining 2 sub- Zone electophoresis, 10L is respectively added.It is powered, the electric field intensity of energization is 80V/cm.It is pure separating
Change in room 10 and set 2 NF membrane 30, the clamping of the NF membrane 30 enters to isolate and purify on the inboard wall groove of room 10, the NF membrane 30
Aperture is 1500 types, 500 types, after the electrical conductivity of the sub- Zone electophoresis 11 near negative electrode 20b tends towards stability, from taking for each Zone electophoresis
Liquid valve 50 take out purification oligochitosan solution, through spray drying obtain oligochitosan product, through HPLC detection, from anode 20a to
The oligochitosan product degree of polymerization type of each sub- Zone electophoresis 11 in negative electrode 20b directions and yield are respectively：10-9 sugar 94%, 8-3 is sugared
92%, 2 sugar 96%.
The oligochitosan of the present invention isolates and purifies device, and simple structure is reasonable in design, isolates and purifies oligochitosan sample yield
It is high；The oligochitosan isolation and purification method process is simple of the present invention, product yield is high, can in high volume isolate and purify oligochitosan sample,
It is easy to the realization of large-scale production；The use of organic reagent is saved simultaneously, the discharge of discarded organic reagent is reduced, and reduces environment
Pollution.
The above, the only specific embodiment of the present invention, but protection scope of the present invention is not limited thereto, any
Those familiar with the art the invention discloses technical scope in, change or replacement can be readily occurred in, all should contain
Cover within protection scope of the present invention.Therefore, protection scope of the present invention described should be defined by scope of the claims.
Claims (9)
1. a kind of oligochitosan isolates and purifies device, including：
Room is isolated and purified, to putting part oligosaccharide solution；
Negative electrode, is arranged at and described isolates and purifies interior；
Anode, be arranged at it is described isolate and purify interior, wherein the anode is arranged with cathode separation, between the anode, negative electrode
Surround Zone electophoresis；
At least one NF membrane, is arranged in the Zone electophoresis so that the Zone electophoresis are separated into at least two sub- Zone electophoresis, when
When NF membrane quantity is 2 or more, each nanofiltration membrane aperture is successively decreased from anode to cathode direction.
2. oligochitosan according to claim 1 isolates and purifies device, it is characterised in that described also including counterflow-preventing baffle plate
Counterflow-preventing baffle plate is located at each NF membrane near the side of negative electrode.
3. oligochitosan according to claim 1 isolates and purifies device, it is characterised in that also include testing sub- Zone electophoresis
The conductivity meter of interior oligochitosan electrophoresis liquid conductivity value.
4. oligochitosan according to claim 1 isolates and purifies device, it is characterised in that also include molten for purification oligochitosan
The liquid taking valve that liquid flows out.
5. a kind of oligochitosan using as described in any one in Claims 1 to 4 isolates and purifies device and oligochitosan is carried out point
From the method for purification, it is characterised in that comprise the following steps：
Oligochitosan solution is injected into into oligochitosan to isolate and purify in device, electrophoresis is obtaining the purification oligochitosan in each sub- Zone electophoresis
Solution.
6. method according to claim 5, it is characterised in that also include：Using the purification oligochitosan solution for obtaining as shell
Oligosaccharide sample carries out secondarily purified, and detached different molecular weight magnitude range is selected not according to required for purification oligochitosan solution
With the NF membrane in aperture, it is further purified.
7. method according to claim 5, it is characterised in that also including dry step.
8. method according to claim 5, it is characterised in that the electrophoresis step includes：
By the injection of oligochitosan solution in the sub- Zone electophoresis of anode；
When electrophoretic liquid concentration reaches predetermined value, it is powered, the electric field intensity value is 20～80V/cm；
After the conductivity meter reading of the sub- Zone electophoresis near negative electrode tends towards stability, power supply is closed.
9. method according to claim 8, it is characterised in that the oligochitosan solution concentration is 0.3-15%.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710148201.XA CN106621810B (en) | 2017-03-14 | 2017-03-14 | Chitosan oligosaccharide separation and purification device and method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710148201.XA CN106621810B (en) | 2017-03-14 | 2017-03-14 | Chitosan oligosaccharide separation and purification device and method thereof |
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Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000071999A1 (en) * | 1999-05-19 | 2000-11-30 | Bilatec Ag | Device and method for isolating electrically charged molecules |
| WO2001078877A1 (en) * | 2000-04-18 | 2001-10-25 | Gradipore Limited | Electrophoresis separation and treatment of samples |
| CN1821276A (en) * | 2006-02-28 | 2006-08-23 | 北京大学 | Device and its process for extracting hyaluronic acid by electrolemma separating technology |
| CN101716465A (en) * | 2009-12-02 | 2010-06-02 | 中国科学院过程工程研究所 | Method and device for separating electric field-separation membrane coupling |
| CN102824854A (en) * | 2011-06-15 | 2012-12-19 | 杜权 | Electrophoresis apparatus and its application |
| CN203577651U (en) * | 2013-11-15 | 2014-05-07 | 华南理工大学 | Device for enhancing inner pressure membrane filtration by using pulse electric fields |
-
2017
- 2017-03-14 CN CN201710148201.XA patent/CN106621810B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000071999A1 (en) * | 1999-05-19 | 2000-11-30 | Bilatec Ag | Device and method for isolating electrically charged molecules |
| WO2001078877A1 (en) * | 2000-04-18 | 2001-10-25 | Gradipore Limited | Electrophoresis separation and treatment of samples |
| CN1821276A (en) * | 2006-02-28 | 2006-08-23 | 北京大学 | Device and its process for extracting hyaluronic acid by electrolemma separating technology |
| CN101716465A (en) * | 2009-12-02 | 2010-06-02 | 中国科学院过程工程研究所 | Method and device for separating electric field-separation membrane coupling |
| CN102824854A (en) * | 2011-06-15 | 2012-12-19 | 杜权 | Electrophoresis apparatus and its application |
| CN203577651U (en) * | 2013-11-15 | 2014-05-07 | 华南理工大学 | Device for enhancing inner pressure membrane filtration by using pulse electric fields |
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| CN106621810B (en) | 2023-07-04 |
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