CN106619695A - Chinese herbal medicinal polysaccharide composition and applications thereof - Google Patents
Chinese herbal medicinal polysaccharide composition and applications thereof Download PDFInfo
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- CN106619695A CN106619695A CN201610957683.9A CN201610957683A CN106619695A CN 106619695 A CN106619695 A CN 106619695A CN 201610957683 A CN201610957683 A CN 201610957683A CN 106619695 A CN106619695 A CN 106619695A
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- 229920001282 polysaccharide Polymers 0.000 title claims abstract description 119
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- 239000003814 drug Substances 0.000 claims abstract description 59
- 206010028980 Neoplasm Diseases 0.000 claims abstract description 45
- 235000013402 health food Nutrition 0.000 claims abstract description 24
- 230000002265 prevention Effects 0.000 claims abstract description 19
- 241000222336 Ganoderma Species 0.000 claims abstract description 18
- 238000009098 adjuvant therapy Methods 0.000 claims abstract description 8
- 239000000463 material Substances 0.000 claims abstract description 7
- 241000756042 Polygonatum Species 0.000 claims description 64
- 235000008737 Polygonatum biflorum Nutrition 0.000 claims description 64
- 238000002360 preparation method Methods 0.000 claims description 27
- 235000013305 food Nutrition 0.000 claims description 23
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 22
- 244000241838 Lycium barbarum Species 0.000 claims description 19
- 235000015459 Lycium barbarum Nutrition 0.000 claims description 19
- 238000005352 clarification Methods 0.000 claims description 17
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- 238000005119 centrifugation Methods 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
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- 238000009777 vacuum freeze-drying Methods 0.000 claims description 5
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- 239000003112 inhibitor Substances 0.000 claims description 4
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- 235000013399 edible fruits Nutrition 0.000 abstract 2
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- 102100022338 Integrin alpha-M Human genes 0.000 description 1
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- 235000010326 Osmanthus heterophyllus Nutrition 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
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- 150000001720 carbohydrates Chemical class 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- -1 compound Polysaccharide Chemical class 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
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- 239000012642 immune effector Substances 0.000 description 1
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- 230000007365 immunoregulation Effects 0.000 description 1
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- LWYXLXAMDLNBFQ-UHFFFAOYSA-N iso-6-Carnavalin Natural products CC(O)CCCCCCCCCCC1CCC(O)C(C)N1 LWYXLXAMDLNBFQ-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
- A61K36/074—Ganoderma
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/81—Solanaceae (Potato family), e.g. tobacco, nightshade, tomato, belladonna, capsicum or jimsonweed
- A61K36/815—Lycium (desert-thorn)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/896—Liliaceae (Lily family), e.g. daylily, plantain lily, Hyacinth or narcissus
- A61K36/8969—Polygonatum (Solomon's seal)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to the field of health food, and in particular relates to a Chinese herbal medicinal polysaccharide composition and applications of the Chinese herbal medicinal polysaccharide composition. The Chinese herbal medicinal composition provided by the invention is composed of rhizoma polygonati, Chinese wolfberry fruits and lucid ganoderma, or is composed of polysaccharide of rhizoma polygonati, polysaccharide of Chinese wolfberry fruits, and polysaccharide of lucid ganoderma. The experiment shows that the prepared traditional Chinese medicinal composition, medicine or health food is prepared through scientific compatibility of the medicinal materials, the medicinal materials complement each other, and no toxic and side effects exist; the Chinese herbal medicinal polysaccharide composition has a nutrition value, also has the effect of recovering the normal immunological surveillance function of the body, and has the effects of prevention and/or adjuvant therapy on tumor.
Description
Technical field
The present invention relates to field of health care food, more particularly to a kind of polysaccharides of traditional Chinese medicine composition and application thereof.
Background technology
There is limitation in existing oncotherapy.Tumour occupy at present the first place of cause of the death spectrum, becomes great health threat, swells
Three big conventional therapies of knurl：Operation, chemicotherapy can be caused with certain limitation, and certain pain sensation is caused to patient
Receive, chemicotherapy also has different degrees of inhibitory action to normal immune system.
Immunization therapy is most important.With the progress of tumor immunology, it is increasingly recognised that immune system pair
In tumour monitoring and remove it is critical that, immunization therapy is described as being expected to the 4th class treatment method for capturing cancer.
Immune negative regulation cell becomes novel targets.In the past of concern is positive immunity, i.e., immunocyte is thin to tumour
The direct killing of born of the same parents, and now academia thinks, tumour cell realize immunologic escape be tumour be able to occur development it is important before
Carry, and the important biomolecule event is that an immune negative regulation network is there is in tumor microenvironment the reason for generation, in the network
Immune negative regulation cell and the factor inhibit monitoring and removing of the immune effector cell to tumour cell.Marrow source SC
(MDSC), regulatory T cells (Treg) are main immune negative regulation cells.
Polysaccharides of traditional Chinese medicine can improve tumor microenvironment.Polysaccharide is the main component of Chinese herbal medicine extract, and some Chinese herbal medicines are more
Sugar can regulate and control activation, propagation and the differentiation of immunocyte, and research in recent years shows that polysaccharides of traditional Chinese medicine is to immune negative regulation cell
Differentiation there is regulating and controlling effect, and then affect its function, performance improves the effect of tumor microenvironment.But have no corresponding Chinese medicine group
The relevant report of compound.
The content of the invention
In view of this, the present invention provides a kind of polysaccharides of traditional Chinese medicine composition and application thereof.Chinese traditional medicine composition obtained by the present invention
Thing, medicine or health food Jing scientific compatibilities are formed, and are brought out the best in each other, and are had no toxic side effect.Both it is nutritious, there is recovery body again
Normal immune surveillance function, can play prevention and/or auxiliary therapeutic action to tumour.
In order to realize foregoing invention purpose, the present invention provides technical scheme below：
The invention provides a kind of Chinese medicine composition, is made up of sealwort, matrimony vine and glossy ganoderma.
In some specific embodiments of the present invention, the active ingredient of sealwort described in the Chinese medicine composition is sealwort
Polysaccharide, the active ingredient of the matrimony vine is LBP-X, and the active ingredient of the glossy ganoderma is GL-B.
In some specific embodiments of the present invention, Siberian solomonseal rhizome polysaccharide described in the Chinese medicine composition, the matrimony vine are more
The sugared mass ratio with the GL-B is (1～5):(1～5):(1～5).
In some specific embodiments of the present invention, Siberian solomonseal rhizome polysaccharide described in the Chinese medicine composition, the matrimony vine are more
The sugared mass ratio with the GL-B is (2～4):(2～4):(2～4).
In some specific embodiments of the present invention, Siberian solomonseal rhizome polysaccharide described in the Chinese medicine composition, the matrimony vine are more
The sugared mass ratio with the GL-B is 2:3:3.
The present invention some specific embodiments in, Siberian solomonseal rhizome polysaccharide described in the Chinese medicine composition, LBP-X or
GL-B is comprised the following steps：By sealwort, matrimony vine or glossy ganoderma crush after, in terms of g/mL, according to sealwort, matrimony vine or glossy ganoderma with
The mass volume ratio of water adds water for the ratio of (0.5g～2.0g): 5mL, and heating stirring extracts 2-4h, mistake at 70 DEG C～75 DEG C
The filter residue that filter is obtained continues to extract twice according to above-mentioned solid-liquid ratio, temperature and time, merges No. three extracts, through reduced pressure concentration
Clarification concentrate is obtained with centrifugation, according to clarification concentrate: 95% ethanol=1: the volume ratio of (7～10) is by corresponding 7～10
Times ethanol of volume 95% is added in clarification concentrate, is mixed after 4 DEG C of overnight stands precipitations, and precipitation, Jing Guozhen is collected by centrifugation
Vacuum freecing-dry obtains Siberian solomonseal rhizome polysaccharide, LBP-X or GL-B.
Present invention also offers described Chinese medicine composition prepare release MDSC inhibitor that T cell is bred and/or
Lower the application in the preparation of tumor mice Treg cell proportions.
Present invention also offers the Chinese medicine composition is in the health food and/or medicine that improve immunity function is prepared
Purposes.
Present invention also offers the Chinese medicine composition prepare prevention and/or adjuvant therapy of tumors health food and/
Or the purposes in medicine.
Additionally, present invention also offers a kind of be used to improve human body immune function and/or prevention and/or adjuvant therapy of tumors
Medicine and/or health food, including described Chinese medicine composition and pharmaceutically acceptable auxiliary material.
Present aspect one preferred embodiment in, the formulation of the medicine or health food is capsule, tablet, particle
Agent, oral liquid, powder, tea in bag.
As experiment surprisingly, it has been found that the health food Jing scientific compatibilities obtained by the present invention are formed, bring out the best in each other, nothing
Toxic and side effect.Both it is nutritious, there is the normal immune surveillance function of recovery body again, tumour can be risen and be prevented and/or auxiliary
Help therapeutic action.Experimental verification, the health food of the present invention releases the depression effect that MDSC breeds to T cell；Also see after administration
Observing complex polysaccharide can lower the ratio of Treg cells in tumor-bearing mice spleen.Its meaning is that recovery body is normally immune
There is immunologic escape in function for monitoring, prevention of tumor cell, can be used to treat or prevent by immune negative regulation cell MDSC and Treg
Caused tumour and precancerous lesion；Strengthen chemotherapeutic antitumous effect.
The object of the invention provides a kind of with the health food for recovering the normal immune surveillance function of body and its preparation side
Method, while confirmation and evaluation Siberian solomonseal rhizome polysaccharide, LBP-X, GL-B combination monitor whether have to recovering body normal immunological
Prevention and improvement result.Health food prepared by the present invention is evaluated by recovering the normal immune surveillance function experiment of body
And checking, it should be noted that cited embodiment should not be construed as limiting the invention.
Description of the drawings
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
The accompanying drawing to be used needed for having technology description is briefly described.
Fig. 1 shows that complex polysaccharide suppresses MSC2 propagation；Note：***P<0.05；Complex polysaccharide group compares with control group；
Fig. 2 gives instructions in reply and closes saccharide portion recovery T cell multiplication capacity；Wherein, Fig. 2 (A) shows control group (splenocyte), Fig. 2 (B)
Show control group+canavaline (ConA), Fig. 2 (C) shows that control group+ConA+MSC2, Fig. 2 (D) show control group+ConA+MSC2+GQ,
Fig. 2 (E) shows that control group+ConA+MSC2+LZ, Fig. 2 (F) show that control group+ConA+MSC2+HJ, Fig. 2 (G) show control group+ConA+
MSC2+HJ+GQ+LZ；
Fig. 3 shows that complex polysaccharide lowers Treg ratios in tumor-bearing mice spleen；Wherein, Fig. 3 (A) shows the sample 1 of control group, figure
3 (B) show the sample 2 of control group, and Fig. 3 (C) shows the sample 3 of control group, and Fig. 3 (D) shows the sample 4 of control group, and Fig. 3 (E) shows control
The sample 5 of group, Fig. 3 (F) shows the sample 6 of control group, and Fig. 3 (G) shows complex polysaccharide group, and (Siberian solomonseal rhizome polysaccharide+LBP-X+glossy ganoderma is more
Sugar) sample 1, Fig. 3 (H) shows the sample 2 of complex polysaccharide group (Siberian solomonseal rhizome polysaccharide+LBP-X+GL-B), and Fig. 3 (I) gives instructions in reply conjunction
The sample 3 of polysaccharide group (Siberian solomonseal rhizome polysaccharide+LBP-X+GL-B), Fig. 3 (J) shows complex polysaccharide group, and (Siberian solomonseal rhizome polysaccharide+matrimony vine is more
Sugar+GL-B) sample 4；
Fig. 4 shows that complex polysaccharide strengthens chemotherapeutic antitumous effect；
In Fig. 1～Fig. 4 of the present invention, GQ shows matrimony vine, and LZ shows glossy ganoderma, and HJ shows sealwort.
Specific embodiment
The invention discloses a kind of polysaccharides of traditional Chinese medicine composition and application thereof, those skilled in the art can use for reference interior herein
Hold, be suitably modified technological parameter realization.Specifically, all similar replacements and change are to those skilled in the art
For be it will be apparent that they are considered as being included in the present invention.The method of the present invention and application have passed through preferably enforcement
Example is described, related personnel substantially can in without departing from present invention, spirit and scope to method described herein and
Using be modified or suitably change with combining realizing and apply the technology of the present invention.
The invention provides a kind of Chinese medicine composition, is made up of sealwort, matrimony vine and glossy ganoderma.
In some specific embodiments of the present invention, the active ingredient of sealwort described in Chinese medicine composition is that sealwort is more
Sugar, the active ingredient of the matrimony vine is LBP-X, and the active ingredient of the glossy ganoderma is GL-B.
The present invention some specific embodiments in, Siberian solomonseal rhizome polysaccharide described in Chinese medicine composition, the LBP-X with
The mass ratio of the GL-B is (1～5):(1～5):(1～5).
The present invention some specific embodiments in, Siberian solomonseal rhizome polysaccharide described in Chinese medicine composition, the LBP-X with
The mass ratio of the GL-B is (2～4):(2～4):(2～4).
According to a first aspect of the present invention, there is provided a kind of health-care food composition, the composition includes Siberian solomonseal rhizome polysaccharide, Chinese holly
Qi polysaccharide, GL-B, preferably by weight 2:3:3 ratios mix, and optionally further include medical science or the acceptable load of food
Body, excipient, excipient and/or filler.
Present aspect one preferred embodiment in, the Siberian solomonseal rhizome polysaccharide, LBP-X or GL-B are natural
Or it is artificial synthesized.
According to a second aspect of the present invention, there is provided a kind of preparation method of health-care food composition, methods described includes, mixes
By weight 2:3:The Siberian solomonseal rhizome polysaccharide of 3 ratios, LBP-X and GL-B.
In one preferred embodiment of the invention, the Siberian solomonseal rhizome polysaccharide, LBP-X or GL-B are by such as lower section
Method is obtained：Take the sealwort, the matrimony vine or the glossy ganoderma to crush, according to sealwort, matrimony vine or glossy ganoderma: water=0.5g～2.0g:
The ratio of 5mL adds water, and in terms of g/mL, heating stirring extracts 3h at 70 DEG C～75 DEG C, filters the filter residue for obtaining according to above-mentioned material
Liquor ratio, temperature and time continue to extract twice, merge No. three extracts, and through reduced pressure concentration and centrifugation clarification concentrate is obtained,
According to clarification concentrate: 95% ethanol=1: corresponding 7～10 times of volumes 95% ethanol is added to clarification by 7～10 volume ratio
In concentrate, the overnight stand precipitation at 4 DEG C is mixed, precipitation is collected by centrifugation, obtain sealwort through vacuum freeze drying many
Sugar, LBP-X or GL-B.
According to a third aspect of the present invention, there is provided a kind of health-care food composition prepared according to the method for second aspect.
According to a fourth aspect of the present invention, there is provided a kind of composition and/or Chinese traditional medicine composition obtained in described preparation method
Thing answering in the preparation for releasing the MDSC inhibitor bred to T cell and/or lowering tumor mice Treg cell proportions is prepared
With.
Present invention also offers described Chinese medicine composition and/or Chinese medicine composition obtained in described preparation method are in system
Purposes in the standby health food and/or medicine for improving immunity function.
Present invention also offers described Chinese medicine composition and/or Chinese medicine composition obtained in described preparation method are in system
Purposes in the health food and/or medicine of standby prevention and/or adjuvant therapy of tumors.
Present invention also offers prepared by described Chinese medicine composition and/or Chinese medicine composition obtained in the preparation method
Recover the purposes in the medicine of the normal immune surveillance function of body.
It is described to recover the normal immune surveillance function of body, including the depression effect that MDSC breeds to T cell is released, recover
The ability of T cell propagation；Treg cell proportions in tumor mice spleen are lowered, improves immune state；Treatment prevents by immunity
Disease caused by negative regulation cell MDSC and Treg, the disease includes tumour and the precancerosis caused by immunosuppressive condition
Become；Strengthen chemotherapeutic antitumous effect.
Present invention also offers it is a kind of for improve immunity function and/or prevention and/or adjuvant therapy of tumors medicine and/
Or health food, including Chinese medicine composition described obtained in described Chinese medicine composition and/or the preparation method and medicine
Acceptable auxiliary material on.
Present aspect one preferred embodiment in, the formulation of the medicine or health food is capsule, tablet, particle
Agent, oral liquid, powder, tea in bag.
As experiment surprisingly, it has been found that the health food Jing scientific compatibilities obtained by the present invention are formed, bring out the best in each other, nothing
Toxic and side effect.Both it is nutritious, there is the normal immune surveillance function of recovery body again, tumour can be risen and be prevented and/or auxiliary
Help therapeutic action.Experimental verification, the health food of the present invention releases the depression effect that MDSC breeds to T cell；Also see after administration
Observing complex polysaccharide can lower the ratio of Treg cells in tumor-bearing mice spleen.Its meaning is that recovery body is normally immune
There is immunologic escape in function for monitoring, prevention of tumor cell, can be used to treat or prevent by immune negative regulation cell MDSC and Treg
Caused tumour and precancerous lesion；Strengthen chemotherapeutic antitumous effect.
The object of the invention provides a kind of with the health food for recovering the normal immune surveillance function of body and its preparation side
Method, while confirmation and evaluation Siberian solomonseal rhizome polysaccharide, LBP-X, GL-B combination monitor whether have to recovering body normal immunological
Prevention and improvement result.Health food prepared by the present invention is evaluated by recovering the normal immune surveillance function experiment of body
And checking, it should be noted that cited embodiment should not be construed as limiting the invention.
Chinese medicine composition that the present invention is provided and preparation method thereof, purposes and the medicine containing the Chinese medicine composition, guarantor
Raw materials used and reagent can be buied by market in health food.
With reference to embodiment, the present invention is expanded on further：
The preparation of the Siberian solomonseal rhizome polysaccharide of embodiment 1
Siberian solomonseal rhizome polysaccharide of the present invention is prepared by following preparation method：After sealwort is crushed, according to sealwort: water=
0.5g: 5mL ratio adds water, at 70 DEG C heating stirring extract 2h, filter obtain filter residue according to above-mentioned solid-liquid ratio, temperature and
Time continues to extract twice, merges No. three extracts, and through reduced pressure concentration and centrifugation clarification concentrate is obtained, according to clarification concentration
Liquid: 95% ethanol=1: 7 volume ratio is added to 7 times of ethanol of volume 95% in clarification concentrate, mixes at 4 DEG C overnight
Staticly settle, precipitation is collected by centrifugation, through vacuum freeze drying Siberian solomonseal rhizome polysaccharide is obtained.
The preparation of the LBP-X of embodiment 2
LBP-X of the present invention is prepared by following preparation method：After matrimony vine is crushed, according to matrimony vine: water=
1g: 5mL ratio adds water, at 75 DEG C heating stirring extract 3h, filter obtain filter residue according to above-mentioned solid-liquid ratio, temperature and when
Between continue extract twice, merge No. three extracts, through reduced pressure concentration and centrifugation obtain clarification concentrate, according to clarification concentrate
: 95% ethanol=1: 9 volume ratio is added to 9 times of ethanol of volume 95% in clarification concentrate, mixes overnight quiet at 4 DEG C
Precipitation is put, precipitation is collected by centrifugation, through vacuum freeze drying LBP-X is obtained.
The preparation of the GL-B of embodiment 3
LBP-X of the present invention, GL-B are prepared by following preparation method：After glossy ganoderma is crushed, according to
Glossy ganoderma: the ratio of water=2g: 5mL adds water, heating stirring extracts 4h at 75 DEG C, filters the filter residue for obtaining according to above-mentioned feed liquid
Continue to extract twice than, temperature and time, merge No. three extracts, through reduced pressure concentration and centrifugation clarification concentrate is obtained, press
According to clarification concentrate: 95% ethanol=1: 10 volume ratio is added to 10 times of ethanol of volume 95% in clarification concentrate, mixes
Overnight stand precipitation, is collected by centrifugation precipitation at 4 DEG C, and through vacuum freeze drying GL-B is obtained.
The preparation of the Siberian solomonseal rhizome polysaccharide of embodiment 4, LBP-X and GL-B composition
By Siberian solomonseal rhizome polysaccharide, embodiment 2 obtained in embodiment 1 LBP-X and GL-B obtained in embodiment 3 is obtained with
Weight meter ratio 2:3:3 mixing, further add medical science or food acceptable carrier, excipient, excipient and/or filling
Agent.
Make the health-care food composition comprising Siberian solomonseal rhizome polysaccharide, LBP-X and GL-B.
The preparation of the Siberian solomonseal rhizome polysaccharide of embodiment 5, LBP-X and GL-B composition
By Siberian solomonseal rhizome polysaccharide, embodiment 2 obtained in embodiment 1 LBP-X and GL-B obtained in embodiment 3 is obtained with
Weight meter ratio 1:5:5 mixing, further add medical science or food acceptable carrier, excipient, excipient and/or filling
Agent.
Make the health-care food composition comprising Siberian solomonseal rhizome polysaccharide, LBP-X and GL-B.
The preparation of the Siberian solomonseal rhizome polysaccharide of embodiment 6, LBP-X and GL-B composition
By Siberian solomonseal rhizome polysaccharide, embodiment 2 obtained in embodiment 1 LBP-X and GL-B obtained in embodiment 3 is obtained with
Weight meter ratio 5:1:1 mixing, further adds medical science or food acceptable carrier, excipient, excipient and/or filling
Agent.
Make the health-care food composition comprising Siberian solomonseal rhizome polysaccharide, LBP-X and GL-B.
The preparation of the Siberian solomonseal rhizome polysaccharide of embodiment 7, LBP-X and GL-B composition
By Siberian solomonseal rhizome polysaccharide, embodiment 2 obtained in embodiment 1 LBP-X and GL-B obtained in embodiment 3 is obtained with
Weight meter ratio 2:4:3 mixing, further add medical science or food acceptable carrier, excipient, excipient and/or filling
Agent.
Make the health-care food composition comprising Siberian solomonseal rhizome polysaccharide, LBP-X and GL-B.
The preparation of the Siberian solomonseal rhizome polysaccharide of embodiment 8, LBP-X and GL-B composition
By Siberian solomonseal rhizome polysaccharide, embodiment 2 obtained in embodiment 1 LBP-X and GL-B obtained in embodiment 3 is obtained with
Weight meter ratio 4:2:4 mixing, further add medical science or food acceptable carrier, excipient, excipient and/or filling
Agent.
Make the health-care food composition comprising Siberian solomonseal rhizome polysaccharide, LBP-X and GL-B.
The preparation of the Siberian solomonseal rhizome polysaccharide of embodiment 9, LBP-X and GL-B composition
By Siberian solomonseal rhizome polysaccharide, embodiment 2 obtained in embodiment 1 LBP-X and GL-B obtained in embodiment 3 is obtained with
Weight meter ratio 3:3:2 mixing, further add medical science or food acceptable carrier, excipient, excipient and/or filling
Agent.
Make the health-care food composition comprising Siberian solomonseal rhizome polysaccharide, LBP-X and GL-B.
The polysaccharide composition of embodiment 10 is to prevention or the checking of adjuvant therapy of tumors function
Present invention applicant uses the Siberian solomonseal rhizome polysaccharide obtained by above-described embodiment 1～4, LBP-X, GL-B, compound
Polysaccharide has carried out cytologic experiment, and experimental result is as follows：
1 experiment material
1.1 laboratory apparatus
Flow cytometer：BD companies, Calibo；
Assay balance：Beijing Sai Duolisi Co., Ltds product, Sartorius BS124S types；
Electronic balance：Beijing Sai Duolisi Co., Ltds product, Sartorius BL1500 types.
1.2 experiment reagents
CFSESigma is purchased from company.CD4+, CD8+, CD25+ streaming antibody is purchased from BD companies.
1.3 Experimental agents
The cell experiment resourcing manifest of table 1
2 experimental techniques
The effect that 2.1 complex polysaccharides are bred to MDSC
MSC2 is inoculated in 96 orifice plates, and cell density is 1 × 104/ hole.Siberian solomonseal rhizome polysaccharide, LBP-X and GL-B are added
In cultivating system.The polysaccharide concentration of single polysaccharide group is respectively 1mg/ml, and complex polysaccharide group total concentration is 1mg/ml, wherein every kind of
Polysaccharide concentration ratio is 2：3：3.After effect 3 days, MTT detections are carried out.
2.2 mouse junction cancer subcutaneous transplantation knurl model constructions and complex polysaccharide are intervened in vitro
Colon carcinoma cell line CT26, in Balb/c mouse, dosage of inoculation is 1 × 10 for subcutaneous vaccination6/ only, after 21 days, put to death
Mouse, takes spleen and prepares single cell suspension, by 4 × 106/ hole is inoculated in 96 orifice plates, and experimental group is to add to be combined in cultivating system
Polysaccharide (Siberian solomonseal rhizome polysaccharide+LBP-X+GL-B), control group includes not adding the list of polysaccharide group (normal group) and 3 kinds of polysaccharide
With group (single polysaccharide group).Effect carries out flow cyctometry detection after 1 day, detection CD11b+Gr1+ cells are in monocyte
Ratio.
2.3 complex polysaccharides are combined with chemotherapeutic
Colon carcinoma cell line CT26, in Balb/c mouse, dosage of inoculation is 1 × 10 for subcutaneous vaccination6/ only.After 12 days, adopt
Complex polysaccharide (Siberian solomonseal rhizome polysaccharide+LBP-X+GL-B) gavage, once a day.Meanwhile, hypodermic injection 5 FU 5 fluorouracil
(5Fu), dosage is 5 mg kg days.Complex polysaccharide dosage is 500 mg kg days.Control group adopts distilled water gavage,
Daily also hypodermic injection 5Fu, dosage is with complex polysaccharide group.Every group of Sample size is 8.When the 20th day, record tumor size is (long
And width), calculating gross tumor volume, formula is 1/2 × it is long × wide2。
3 experimental results
The effect that 3.1 complex polysaccharides are bred to MSC2, is shown in Fig. 1, table 2.
As a result show, complex polysaccharide group MDSC propagation is considerably less than control group, also less than single polysaccharide group.The explanation of this result
Complex polysaccharide (Siberian solomonseal rhizome polysaccharide+LBP-X+GL-B) can produce Synergistic, suppress MDSC, improve the immunity of body
State, can be used to prepare health products and food, can be used for prevention and the auxiliary treatment of tumour.
The depression effect that the complex polysaccharide of table 2 is bred to MDSC
Note：*：Complex polysaccharide compares with control group, P ＜ 0.01；#：Complex polysaccharide compares with each single polysaccharide group, P ＜
0.01。
T cell expanding capacity is recovered in 3.2 complex polysaccharide parts, sees Fig. 2, table 3.
As a result show, complex polysaccharide group T cell propagation obtains part recovery, propagation peak occur, and single polysaccharide group does not go out
Now breed peak.This result explanation complex polysaccharide (Siberian solomonseal rhizome polysaccharide+LBP-X+GL-B) can be thin to T with past release MDSC
The inhibitory action of born of the same parents' propagation, plays immunoregulation effect, can be used to prepare health products and food, can be used for the prevention of tumour and auxiliary
Help treatment.
The effect of the suppression that the complex polysaccharide of table 3 reverse MDSC breeds to T cell
Note：*：Complex polysaccharide compares with control group, P ＜ 0.01；#：Complex polysaccharide compares with single polysaccharide group, P ＜ 0.01.
3.3 complex polysaccharides lower colon cancer CT26 tumor-bearing mice Treg cell proportions, see Fig. 3, table 4.
As a result show, complex polysaccharide group Treg cell proportion is less than control group.This result illustrates complex polysaccharide (Siberian solomonseal rhizome polysaccharide
+ LBP-X+GL-B) tumor mice Treg cell proportions can be lowered, improve the immune state of body, can be used to prepare
Health products and food, can be used for prevention and the auxiliary treatment of tumour.
The complex polysaccharide of table 4 lowers the ratio of Treg in CT26 tumor-bearing mice spleens
Note：*：Complex polysaccharide compares with control group, P ＜ 0.01；#：Complex polysaccharide compares with single polysaccharide group, P ＜ 0.05.
3.4 complex polysaccharides collaboration chemotherapeutic is antitumor, sees Fig. 4, table 5.
As a result show, when the 20th day, complex polysaccharide and chemotherapeutic combination group, colon cancer tumours volume is less than chemotherapy
Group.This result explanation complex polysaccharide (Siberian solomonseal rhizome polysaccharide+LBP-X+GL-B) can strengthen the antitumous effect of chemotherapeutic,
Can be used for the auxiliary treatment of chemotherapy of tumors.
The effect of inhibitor against colon carcinoma cells in the complex polysaccharide body of table 5
Note：*：Complex polysaccharide compares with control group, P ＜ 0.01.
Based on the above results, the Siberian solomonseal rhizome polysaccharide of the preparation of embodiment 4, LBP-X, GL-B composition can be released
The depression effect that MDSC breeds to T cell；It was additionally observed that complex polysaccharide can lower the ratio of Treg cells in tumor-bearing mice spleen
Example.Its meaning is to recover the normal immune surveillance function of body, prevention of tumor cell occurs immunologic escape, and prevention of tumor is sent out
It is raw.Thus, show the embodiment of the present invention 4 prepare Siberian solomonseal rhizome polysaccharide, LBP-X, GL-B composition have recover body just
Normal immune surveillance function, and have more advantage (p than Siberian solomonseal rhizome polysaccharide, LBP-X, GL-B is used alone<0.05).
The Siberian solomonseal rhizome polysaccharide of the preparation of 5～embodiment of Example 9, LBP-X, GL-B composition carry out above-mentioned experiment,
Experimental result is same or like with the result of composition prepared by embodiment 4, has more than Siberian solomonseal rhizome polysaccharide, LBP-X is used alone
Advantage, and with significant difference (P≤0.05).
Had according to the Siberian solomonseal rhizome polysaccharide, LBP-X, GL-B composition and recover the normal immune surveillance function of body,
Siberian solomonseal rhizome polysaccharide, LBP-X, glossy ganoderma made by natural, artificial and above-described embodiment 1,2 and 3 preparation method is more
Sugar, prepares in proportion (composition that i.e. prepared by embodiment 4, test discovery 2:3：3 proportional arrangement effects are most unexpected), then add
Enter to eat or pharmaceutic adjuvant, the various health foods such as capsule, tablet, granule, oral liquid, powder, tea in bag can be prepared into
Formulation.
The Siberian solomonseal rhizome polysaccharide of the different proportion of embodiment 11, LBP-X and GL-B composition and its effect
By Siberian solomonseal rhizome polysaccharide, embodiment 2 obtained in embodiment 1 LBP-X and GL-B obtained in embodiment 3 is obtained with
Different Weight meter ratio mixing in table 6, further adds medical science or food acceptable carrier, excipient, excipient and/or fills out
Fill agent.Make the health-care food composition comprising Siberian solomonseal rhizome polysaccharide, LBP-X and GL-B.And according to energy in above-described embodiment
Enough verification methods that prevention and/or auxiliary therapeutic action are played to tumour, be restored body normal immunological function for monitoring effect
As a result score value.(100 points of systems, with embodiment 42:3:The 3 mixing quality body that recovers more corresponding than prepared composition is normally exempted from
Epidemic disease function for monitoring effect is designated as 100 points, and the corresponding function test result of other mixed proportions compares the function test for accounting for embodiment 4
As a result percentage point, is designated as corresponding function score value).
The Siberian solomonseal rhizome polysaccharide of the different proportion of table 6, LBP-X and GL-B composition and its effect
Function test result finds Siberian solomonseal rhizome polysaccharide, LBP-X and GL-B in (1～5):(1～5):(1～5) it is mixed
Compare during composition and division in a proportion example and individually apply three kinds of polysaccharide with body normal immunological function for monitoring is more significantly recovered, tumour is risen pre-
Anti- and/or auxiliary therapeutic action.It is (2～4) when polysaccharide mixed proportion is applied:(2～4):When (2～4), effect is more notable.
Health food Jing scientific compatibilities of the present invention are formed, and are brought out the best in each other, and are had no toxic side effect；It was both nutritious,
There is the function of recovering the monitoring of body normal immunological again.Applicant states that above content is to combine specific embodiment to this
Bright made further description, it is impossible to assert the present invention be embodied as be confined to these explanations.For institute of the present invention
For the those of ordinary skill of category technical field, without departing from the inventive concept of the premise, can also make and some simply push away
Drill or replace, should all be considered as belonging to protection scope of the present invention.
Claims (10)
1. a kind of Chinese medicine composition, it is characterised in that be made up of sealwort, matrimony vine and glossy ganoderma.
2. Chinese medicine composition according to claim 1, it is characterised in that the active ingredient of the sealwort is Siberian solomonseal rhizome polysaccharide,
The active ingredient of the matrimony vine is LBP-X, and the active ingredient of the glossy ganoderma is GL-B.
3. Chinese medicine composition according to claim 2, it is characterised in that the Siberian solomonseal rhizome polysaccharide, the LBP-X and institute
The mass ratio for stating GL-B is (1～5):(1～5):(1～5).
4. Chinese medicine composition according to claim 2, it is characterised in that the Siberian solomonseal rhizome polysaccharide, the LBP-X and institute
The mass ratio for stating GL-B is (2～4):(2～4):(2～4).
5. Chinese medicine composition according to claim 2, it is characterised in that the Siberian solomonseal rhizome polysaccharide, the LBP-X and institute
The mass ratio for stating GL-B is 2:3:3.
6. the Chinese medicine composition according to any one of claim 2 to 5, it is characterised in that the Siberian solomonseal rhizome polysaccharide, LBP-X
Or GL-B is comprised the following steps：After respectively crushing sealwort, matrimony vine or glossy ganoderma, in terms of g/mL, sealwort, matrimony vine or spirit are taken
Sesame is (0.5～2.0) according to mass volume ratio with water:5 mixing, heating stirring extracts 2～4h at 70 DEG C～75 DEG C, filters and receives
Collection filter residue repeats to extract twice according to above-mentioned solid-liquid ratio, temperature and time, merges No. three extracts, through reduced pressure concentration and centrifugation
Clarification concentrate is obtained, is 1 with the volume ratio of 95% ethanol according to clarification concentrate: (7～10) mix, and staticly settle in 4 DEG C,
Precipitation is collected by centrifugation, through vacuum freeze drying Siberian solomonseal rhizome polysaccharide, LBP-X or GL-B are obtained respectively.
7. the Chinese medicine composition according to any one of claim 1 to 6 is preparing the inhibitor that releasing MDSC breeds to T cell
And/or the application in the preparation of downward tumor mice Treg cell proportions.
8. the Chinese medicine composition according to any one of claim 1 to 6 prepare improve immunity function health food and/or
Purposes in medicine.
9. the Chinese medicine composition according to any one of claim 1 to 6 is preparing the health care of prevention and/or adjuvant therapy of tumors
Purposes in food and/or medicine.
10. it is a kind of for improve human body immune function and/or prevention and/or adjuvant therapy of tumors medicine and/or health food,
Characterized in that, including the Chinese medicine composition as described in any one of claim 1 to 6 and pharmaceutically acceptable auxiliary material.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107375438A (en) * | 2017-09-07 | 2017-11-24 | 无限极（中国）有限公司 | A kind of Chinese medicine composition and application thereof |
| CN107468730A (en) * | 2017-09-07 | 2017-12-15 | 无限极（中国）有限公司 | A kind of Chinese medicine composition and application thereof |
| CN109953999A (en) * | 2019-04-08 | 2019-07-02 | 清华大学 | The composition with immunological enhancement being made of ganoderma lucidum polysaccharide, grifola polysaccharide |
| CN113101341A (en) * | 2021-03-25 | 2021-07-13 | 中国工程物理研究院材料研究所 | Application of rhizoma polygonati in preparation of medicines for preventing and treating low-dose or/and chronic uranium exposure |
-
2016
- 2016-10-27 CN CN201610957683.9A patent/CN106619695A/en not_active Withdrawn
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107375438A (en) * | 2017-09-07 | 2017-11-24 | 无限极（中国）有限公司 | A kind of Chinese medicine composition and application thereof |
| CN107468730A (en) * | 2017-09-07 | 2017-12-15 | 无限极（中国）有限公司 | A kind of Chinese medicine composition and application thereof |
| US10967016B2 (en) | 2017-09-07 | 2021-04-06 | Infinitus (China) Company Ltd. | Traditional Chinese medicine composition and use thereof |
| US11026988B2 (en) | 2017-09-07 | 2021-06-08 | Infinitus (China) Company Ltd. | Traditional Chinese medicine composition and use thereof |
| CN109953999A (en) * | 2019-04-08 | 2019-07-02 | 清华大学 | The composition with immunological enhancement being made of ganoderma lucidum polysaccharide, grifola polysaccharide |
| CN113101341A (en) * | 2021-03-25 | 2021-07-13 | 中国工程物理研究院材料研究所 | Application of rhizoma polygonati in preparation of medicines for preventing and treating low-dose or/and chronic uranium exposure |
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