CN106617227B - A method of preparing beta carotene microcapsules - Google Patents
A method of preparing beta carotene microcapsules Download PDFInfo
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- CN106617227B CN106617227B CN201610813667.2A CN201610813667A CN106617227B CN 106617227 B CN106617227 B CN 106617227B CN 201610813667 A CN201610813667 A CN 201610813667A CN 106617227 B CN106617227 B CN 106617227B
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- cgtase
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- beta carotene
- starch
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- 239000003094 microcapsule Substances 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 17
- OENHQHLEOONYIE-UKMVMLAPSA-N all-trans beta-carotene Natural products CC=1CCCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C OENHQHLEOONYIE-UKMVMLAPSA-N 0.000 title claims description 27
- 239000011648 beta-carotene Substances 0.000 title claims description 27
- 235000013734 beta-carotene Nutrition 0.000 title claims description 27
- TUPZEYHYWIEDIH-WAIFQNFQSA-N beta-carotene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2=CCCCC2(C)C TUPZEYHYWIEDIH-WAIFQNFQSA-N 0.000 title claims description 27
- 229960002747 betacarotene Drugs 0.000 title claims description 27
- OENHQHLEOONYIE-JLTXGRSLSA-N β-Carotene Chemical compound CC=1CCCC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C OENHQHLEOONYIE-JLTXGRSLSA-N 0.000 title claims description 27
- 238000006243 chemical reaction Methods 0.000 claims abstract description 26
- 229920002472 Starch Polymers 0.000 claims abstract description 25
- 235000019698 starch Nutrition 0.000 claims abstract description 25
- 239000008107 starch Substances 0.000 claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 229920000858 Cyclodextrin Polymers 0.000 claims abstract description 19
- 108010025880 Cyclomaltodextrin glucanotransferase Proteins 0.000 claims abstract description 18
- 238000006555 catalytic reaction Methods 0.000 claims abstract description 10
- 108090000790 Enzymes Proteins 0.000 claims abstract description 8
- 102000004190 Enzymes Human genes 0.000 claims abstract description 8
- 241001238055 Bacillus sp. G-825-6 Species 0.000 claims abstract description 5
- 239000000047 product Substances 0.000 claims description 33
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 12
- 239000000758 substrate Substances 0.000 claims description 12
- GDSRMADSINPKSL-HSEONFRVSA-N gamma-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO GDSRMADSINPKSL-HSEONFRVSA-N 0.000 claims description 9
- 238000002360 preparation method Methods 0.000 claims description 9
- 238000009835 boiling Methods 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 7
- 229910052757 nitrogen Inorganic materials 0.000 claims description 6
- 238000002604 ultrasonography Methods 0.000 claims description 6
- 240000003183 Manihot esculenta Species 0.000 claims description 5
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 claims description 5
- 239000008367 deionised water Substances 0.000 claims description 5
- 229910021641 deionized water Inorganic materials 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 239000007787 solid Substances 0.000 claims description 5
- 239000006228 supernatant Substances 0.000 claims description 5
- 238000005119 centrifugation Methods 0.000 claims description 4
- 230000008569 process Effects 0.000 claims description 4
- 238000004108 freeze drying Methods 0.000 claims description 3
- 229920002261 Corn starch Polymers 0.000 claims description 2
- 108010055629 Glucosyltransferases Proteins 0.000 claims description 2
- 102000000340 Glucosyltransferases Human genes 0.000 claims description 2
- 239000011162 core material Substances 0.000 claims description 2
- 239000008120 corn starch Substances 0.000 claims description 2
- 229940099112 cornstarch Drugs 0.000 claims description 2
- 229940080345 gamma-cyclodextrin Drugs 0.000 claims description 2
- 229920001592 potato starch Polymers 0.000 claims description 2
- 238000012545 processing Methods 0.000 claims description 2
- 230000003197 catalytic effect Effects 0.000 abstract description 12
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 9
- 239000001116 FEMA 4028 Substances 0.000 abstract description 5
- 230000001580 bacterial effect Effects 0.000 abstract description 5
- 235000011175 beta-cyclodextrine Nutrition 0.000 abstract description 5
- 229960004853 betadex Drugs 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 241000193830 Bacillus <bacterium> Species 0.000 abstract description 2
- 230000035484 reaction time Effects 0.000 abstract description 2
- -1 that is Proteins 0.000 abstract 1
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 description 13
- 238000005259 measurement Methods 0.000 description 10
- 239000004375 Dextrin Substances 0.000 description 5
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- 229920001353 Dextrin Polymers 0.000 description 4
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 4
- 230000009514 concussion Effects 0.000 description 4
- 235000019425 dextrin Nutrition 0.000 description 4
- 238000004090 dissolution Methods 0.000 description 4
- 239000007789 gas Substances 0.000 description 4
- 230000009849 deactivation Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000005227 gel permeation chromatography Methods 0.000 description 3
- 238000000227 grinding Methods 0.000 description 3
- 238000003556 assay Methods 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 230000031700 light absorption Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 229920000881 Modified starch Polymers 0.000 description 1
- 239000004368 Modified starch Substances 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- PMZURENOXWZQFD-UHFFFAOYSA-L Sodium Sulfate Chemical compound [Na+].[Na+].[O-]S([O-])(=O)=O PMZURENOXWZQFD-UHFFFAOYSA-L 0.000 description 1
- HFHDHCJBZVLPGP-RWMJIURBSA-N alpha-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO HFHDHCJBZVLPGP-RWMJIURBSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000002209 hydrophobic effect Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000019426 modified starch Nutrition 0.000 description 1
- 238000003012 network analysis Methods 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 239000012074 organic phase Substances 0.000 description 1
- 239000003208 petroleum Substances 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N sodium azide Substances [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 1
- 235000010344 sodium nitrate Nutrition 0.000 description 1
- 239000004317 sodium nitrate Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention discloses a kind of methods that the product using cyclodextrin glycosyltransferase, that is, CGTase catalytic starch prepares bata-carotene microcapsules, the γ CGTase of bacterial strain Alkaliphilic bacillus Alkalophilic Bacillus sp.G 825 6 are used for catalytic starch, catalytic condition is preferably starch concentration 4%, enzyme concentration 4U/g, 50 DEG C of reactions are for 24 hours, product after catalysis is directly used in embedding bata-carotene, and embedding conditions are that the mass ratio of Subjective and Objective is 4:1,50 DEG C of reaction temperature, reaction time 3h.It is 51% to the embedding rate of bata-carotene under best embedding conditions, it is suitable with the beta cyclodextrin of document report embedding effect, method is simple by the present invention, the water solubility of CGTase modified outcomes is more than 20 times of beta cyclodextrin, it is high to bata-carotene solubilising multiple, the scope of application is more extensive, and production cost substantially reduces.
Description
Technical field
The invention belongs to technical fields prepared by microcapsules, and in particular to one kind passing through γ-CGTase catalytic starch products
The method for preparing beta carotene microcapsules as wall material.
Background technology
It is reported that cyclodextrin because with hydrophobic, hydrophilic outside ring property in ring, lyophobic dust capable of occurring embedding and
Stabilization is played, is applied to the technical field of microcapsules more and more widely as a kind of good wall material.Cyclodextrin comes more
Derived from the enzymolysis of starch, i.e., it is cyclodextrin by Starch Conversion, at present mostly by cyclodextrin glycosyltransferase (CGTase)
The product that several CGTase effect starch generates is the mixture of tri- kinds of cyclodextrin of α, β, γ.But the effect of CGTase catalytic starch
Rate is not usually high, and as by-product, the phase is separated generally less than 50%, i.e., 50% noncyclic components after the reaction, this is not
It is low only to cause raw material availability, and remaining by-product is difficult directly to be recycled, to further increase cyclodextrin
Cost, seriously limit the development of cyclodextrin industry.Only a small amount of report is using cyclodextrin complexes as embedding main body
Preferable embedding effect is played.
It has been found that γ-the CGTase for coming from strains A lkalophilicBacillus sp.G-825-6 can be catalyzed
Starch generates β-CD and γ-CD, does not generate α-CD, and the ratio of β-CD and γ-CD is 1:1 or so.However, currently without about
The correlation that γ-CGTase Modified Starch products from Alkaliphilic bacillus Bacillus sp.G-825-6 bacterial strains directly utilize
Report does not utilize the product of cyclodextrin glycosyltransferase catalytic starch to be directly used in micro- glue without separation, purifying yet
The relevant report of the embedding of capsule.To sum up, the abundant profit for how being further simplified production technology, reducing production cost, realizing resource
With a great problem for being badly in need of solving as this field.
Invention content
In view of the above-mentioned problems existing in the prior art, current inventor provides one kind is used by CGTase catalytic starch products
The method for preparing beta carotene microcapsules as wall material.
Specific technical solution is as described below：
The present invention provides a kind of methods preparing beta carotene microcapsules, utilize gamma-cyclodextrin glucosyltransferase
That is γ-CGTase carry out catalysis reaction to starch substrates, and the embedding that all products after catalysis are directly used in beta carotene is anti-
It answers, is freeze-dried after reaction, to which beta carotene microcapsules be prepared；
Wherein, γ-CGTase come from Alkaliphilic bacillus Alkalophilic Bacillus sp.G-825-6 bacterial strains,
The starch is tapioca, potato starch or cornstarch；
Above-mentioned catalysis reaction is as follows：It takes starch as substrate, deionized water Heat Gelatinization is added, is made dense
Degree is the solution of 1%-4% (w/v), is cooled to room temperature, takes 1mL substrates that the γ-CGTase isothermal reactions of 2-8U/g, reaction is added
After using boiling water bath carry out destroy the enzyme treatment, centrifugation, take supernatant to be freeze-dried；
Preferably, a concentration of the 4% of above-mentioned starch solution, enzyme concentration 4U/g；
Preferably, the time of the isothermal reaction is 23-25h, and the time of boiling water bath processing is 10-12min, more preferably
Ground, isothermal reaction are placed on isothermal vibration vortex mixer and carry out；
Preferably, it is 5000-5200rpm, time 10-12min that the condition of the centrifugation, which is rotating speed,.
Preferably, freeze-drying is the dry 48h in freeze drier, then collects sample and slightly grinds, is stored in 4 DEG C
In environment, such as it is stored in valve bag and is preserved in 4 DEG C of refrigerator；Preferably, embedding reaction is to be according to mass ratio
1:1-4:1 weigh catalysis reaction after enzymolysis product and beta carotene, using enzymolysis product as wall material, using beta carotene as
Core material, being dissolved in water makes solid content be 24-26%, and nitrogen charging gas shielded is ultrasonically treated, and is reacted after mixing, after reaction
Cooling, freeze-drying obtains microcapsules.
Preferably, the condition of the supersound process is 200-230W ultrasounds 8-12min.
Preferably, the condition reacted after mixing is that 1-5h is reacted at 45-55 DEG C, preferably, reaction time 3h, and more
Preferably, mixing is placed in concussion vortex mixer and carries out, rotating speed 800rpm.
The present invention is beneficial to be had technical effect that：
In the prior art in the technical process for carrying out microcapsules preparation, it will usually select cyclodextrin or starch, not have
Any operating procedure using enzymatic product；The method provided by the present invention for preparing beta carotene microcapsules breaks tradition
Thinking makes all products after CGTase catalytic starch without isolating and purifying, be directly used in embedding, therefore utilization rate is close
100%.
The γ-for enzyme, that is, Alkalophilic Bacillus sp.G-825-6 bacterial strains that the present invention is produced using special bacterial strain
CGTase catalytic starch, the product after enzymolysis be the mixture of β-CD, γ-CD and noncyclic components, the wherein yield of γ-CD about
It is 20% or so, remaining noncyclic components is mainly molecular weight about 105Dextrin.Starch be converted to after enzymatic cyclodextrin and
Dextrin, both substances have embedding effect, but when being used alone, dextrin can not form inclusion completely since chain length is too short
Object, therefore it is bad to embed effect；And the water solubility of β-CD is too poor in cyclodextrin, cannot meet properties of product requirement, γ-CD separation is pure
It is difficult to change step, it is too high so as to cause embedding cost.The step that γ-CD are isolated and purified is eliminated using method provided by the present invention
Suddenly, the common advantage of both cyclodextrin and dextrin is combined, the production cost of cyclodextrin can be reduced, while playing cyclodextrin
Embedding effect, the beta carotene microcapsules prepared with this method are at low cost, and microencapsulation effect is suitable with β-CD, microencapsulation
At low cost, the water solubility of microcapsule product is more preferable.
Description of the drawings
Fig. 1 is the gel chromatography figure that γ-CGTase catalytic starch molecular weight of product measures in embodiment 1.
Specific implementation mode
With reference to the accompanying drawings of the specification and specific implementation mode, technical scheme of the present invention is further specifically described.
Embodiment 1
(1) it takes tapioca as substrate, deionized water Heat Gelatinization is added, the solution of a concentration of 1% (w/v) is made,
Be cooled to room temperature, take 1mL substrates be added 2U/g CGTase, be placed on isothermal vibration vortex mixer and react for 24 hours, after boiling water bath
Enzyme deactivation 10min.Then 5000r/min centrifuges 10min, is put into freeze drier and dries 48h, and after sample collection, slightly grinding is deposited
4 DEG C of refrigerators preserve in valve bag.
(2) it is 1 according to mass ratio:1 weighs enzymolysis product, beta carotene, and water dissolution is then added, makes solid content
It is 25%, nitrogen charging gas shielded, 200W ultrasound 10min are placed in 800r/min in concussion vortex mixer, and 50 DEG C of reaction 1h postcoolings are cold
Be lyophilized it is dry after obtain microcapsules.
Molecular weight is measured using the method for gel chromatography after CGTase catalytic starch products in step (1), concrete outcome is such as
Shown in Fig. 1；It is calculated through analysis and learns that product weight average molecular weight is 4.9 × 105Da, i.e. product after catalysis are main in addition to γ-CD
It is dextrin.
Fig. 1 is the gel chromatography figure that γ-CGTase catalytic starch molecular weight of product measures, and the actual conditions measured are：
The solution for taking sample preparation 1mg/mL is filtered with 0.45 μm of membrane filter (water system), then uses HPSEC-MALLS-RI
Network analysis molecular weight, mobile phase are dissolving 0.02%NaN3The sodium nitrate solution of the 0.3M of solution is maintained at 50 DEG C with 0.5
The flow velocity of ml/min.Measurement result uses ASTRA softwares (version 5.3.4, Wyatt Technology, Santa
Barbara, CA, USA) calculate the weight average molecular weight (Mw) of sample.
Embodiment 2
(1) it takes tapioca as substrate, deionized water Heat Gelatinization is added, the solution of a concentration of 2% (w/v) is made,
Be cooled to room temperature, take 1mL substrates be added 8U/g CGTase, be placed on isothermal vibration vortex mixer and react for 24 hours, after boiling water bath
Enzyme deactivation 10min.Then 5000r/min centrifuges 10min, is put into freeze drier and dries 48h, and after sample collection, slightly grinding is deposited
4 DEG C of refrigerators preserve in valve bag.
(2) it is 2 according to mass ratio:1 weighs enzymolysis product, beta carotene, and water dissolution is then added, makes solid content
It is 25%, nitrogen charging gas shielded, 200W ultrasound 10min are placed in 800r/min in concussion vortex mixer, and 50 DEG C of reaction 5h postcoolings are cold
Be lyophilized it is dry after obtain microcapsules.
Embodiment 3
(1) it takes tapioca as substrate, deionized water Heat Gelatinization is added, the solution of a concentration of 4% (w/v) is made,
Be cooled to room temperature, take 1mL substrates be added 4U/g CGTase, be placed on isothermal vibration vortex mixer and react for 24 hours, after boiling water bath
Enzyme deactivation 10min.Then 5000r/min centrifuges 10min, is put into freeze drier and dries 48h, and after sample collection, slightly grinding is deposited
4 DEG C of refrigerators preserve in valve bag.
(2) it is 4 according to mass ratio:1 weighs enzymolysis product, beta carotene, and water dissolution is then added, makes solid content
It is 25%, nitrogen charging gas shielded, 200W ultrasound 10min are placed in 800r/min in concussion vortex mixer, and 50 DEG C of reaction 3h postcoolings are cold
Be lyophilized it is dry after obtain microcapsules.
The measurement of solubility is carried out to the enzymolysis product prepared in above-described embodiment 1-3 steps (1), and to step
(2) microcapsule product prepared in carries out the measurement of embedding rate, and specific assay method and interpretation of result are as follows：
1, the measurement of solubility
The measurement of solubility is carried out with reference to the assay method of Chinese Pharmacopoeia bulk pharmaceutical chemicals solubility.
2, the measurement of embedding rate
Beta carotene total amount-product surface beta carotene amount in beta carotene amount=product in inclusion compound
The measurement of beta carotene total amount in product：The 0.02g embedding a small amount of water dissolutions of product are weighed, acetone is added：Oil
Ether (1:1) solution 200W ultrasounds 10min, makes carrotene be transferred in organic phase, is dehydrated and is centrifuged with anhydrous sodium sulfate, then fixed
Hold, until 330nm measures light absorption value.
The measurement of product surface beta carotene amount：It weighs 0.02g embedding products and 5mL or so n-hexane is added, shake 1min
Left and right centrifuging and taking supernatant, wash repeatedly 2-3 times becomes faint yellow or colourless to supernatant liquor, then merges supernatant liquor,
It is dried up with nitrogen, beta carotene is redissolved in acetone：Petroleum ether (1:1) in solution, constant volume measures light absorption value.
Measurement result is as shown in table 1, and wherein reference examples select beta-cyclodextrin to carry out the embedding of microcapsules for wall material, determine
The solubility of beta-cyclodextrin and the embedding rate of beta carotene microcapsules, the specific experiment method and result of reference examples measure ginseng
See reference document：In research [J] of Chen Fahe, Wu Guangbin, Chen Zhi brightness natural Beta-carotene beta-cyclodextrin microcapsule preparation process
State's food journal, 2006,6 (1)：110-115.
The measurement result of the solubility and microcapsule embedded rate of 1 wall material of table
| Process | Solubility (unit：g/100ml) | Embedding rate (unit：%) |
| Embodiment 1 | 27 | 47.63 |
| Embodiment 2 | 27 | 50.08 |
| Embodiment 3 | 28 | 51.15 |
| Reference examples | 1.89 | 43.40 |
The above described is only a preferred embodiment of the present invention, being not intended to limit the present invention in any form, appoint
What is simply repaiied to any made by above example according to the technical essence of the invention without departing from technical solution of the present invention content
Change, equivalent variations and modification, in the range of still falling within technical solution of the present invention.
Claims (7)
1. a kind of method preparing beta carotene microcapsules, which is characterized in that be using gamma-cyclodextrin glucosyltransferase
γ-CGTase carry out catalysis reaction to starch substrates, and the embedding that all products after catalysis are directly used in beta carotene is anti-
It answers, is freeze-dried after reaction, to which beta carotene microcapsules be prepared；
γ-the CGTase come from strains A lkalophilic Bacillus sp.G-825-6；
The catalysis reaction is as follows：It takes starch as substrate, deionized water Heat Gelatinization is added, mass body is made
The solution of a concentration of 1%-4% of product ratio, is cooled to room temperature, takes 1mL substrates that the γ-CGTase isothermal reactions of 2-8U/g are added,
Boiling water bath is used to carry out destroy the enzyme treatment after reaction；Centrifugation, takes supernatant to be freeze-dried；
It is 1 that the embedding reaction, which is according to mass ratio,:1-4:1 weighs the enzymolysis product and beta carotene after catalysis reaction, with
Enzymolysis product is as wall material, and using beta carotene as core material, being dissolved in water makes solid content be 24-26%, and inflated with nitrogen is protected
Shield is ultrasonically treated, and is reacted after mixing, is cooled down after reaction, and freeze-drying obtains microcapsules.
2. preparation method according to claim 1, which is characterized in that the starch be tapioca, potato starch or
Cornstarch.
3. preparation method according to claim 1, which is characterized in that the time of the isothermal reaction is 23-25h, boiling water
The time of bath processing is 10-12min.
4. preparation method according to claim 1, which is characterized in that the condition of the centrifugation is that rotating speed is 5000-
5200rpm, time 10-12min.
5. preparation method according to claim 1, which is characterized in that ground after the sample collection after being freeze-dried
Mill, is then stored in 4 DEG C of environment.
6. preparation method according to claim 1, which is characterized in that the condition of the supersound process is 200-230W ultrasounds
8-12min。
7. preparation method according to claim 1, which is characterized in that the condition reacted after mixing is anti-at 45-55 DEG C
Answer 1-5h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610813667.2A CN106617227B (en) | 2016-09-09 | 2016-09-09 | A method of preparing beta carotene microcapsules |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610813667.2A CN106617227B (en) | 2016-09-09 | 2016-09-09 | A method of preparing beta carotene microcapsules |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106617227A CN106617227A (en) | 2017-05-10 |
| CN106617227B true CN106617227B (en) | 2018-09-18 |
Family
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