CN106616147A - Preparation method of nutrient beverage containing bombyxmor protein - Google Patents
Preparation method of nutrient beverage containing bombyxmor protein Download PDFInfo
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- CN106616147A CN106616147A CN201611044902.0A CN201611044902A CN106616147A CN 106616147 A CN106616147 A CN 106616147A CN 201611044902 A CN201611044902 A CN 201611044902A CN 106616147 A CN106616147 A CN 106616147A
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- preparation
- bombyxmor
- pupa albumen
- weight
- nutritious drink
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- 238000002360 preparation method Methods 0.000 title claims abstract description 22
- 235000015097 nutrients Nutrition 0.000 title abstract description 6
- 108090000623 proteins and genes Proteins 0.000 title abstract description 5
- 102000004169 proteins and genes Human genes 0.000 title abstract description 5
- 235000013361 beverage Nutrition 0.000 title abstract 5
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims abstract description 21
- 239000007864 aqueous solution Substances 0.000 claims abstract description 20
- 239000001509 sodium citrate Substances 0.000 claims abstract description 13
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 claims abstract description 13
- 239000000243 solution Substances 0.000 claims abstract description 13
- 108010059892 Cellulase Proteins 0.000 claims abstract description 7
- 229940106157 cellulase Drugs 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims description 36
- 241000255789 Bombyx mori Species 0.000 claims description 32
- 241000382353 Pupa Species 0.000 claims description 31
- 239000012634 fragment Substances 0.000 claims description 22
- 235000008935 nutritious Nutrition 0.000 claims description 18
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 12
- 238000011033 desalting Methods 0.000 claims description 12
- NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 claims description 6
- 229920001353 Dextrin Polymers 0.000 claims description 6
- 239000004375 Dextrin Substances 0.000 claims description 6
- FEWJPZIEWOKRBE-JCYAYHJZSA-N Dextrotartaric acid Chemical compound OC(=O)[C@H](O)[C@@H](O)C(O)=O FEWJPZIEWOKRBE-JCYAYHJZSA-N 0.000 claims description 6
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 claims description 6
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 claims description 6
- 235000010323 ascorbic acid Nutrition 0.000 claims description 6
- 229960005070 ascorbic acid Drugs 0.000 claims description 6
- 239000011668 ascorbic acid Substances 0.000 claims description 6
- 235000019425 dextrin Nutrition 0.000 claims description 6
- 239000003995 emulsifying agent Substances 0.000 claims description 6
- 239000012467 final product Substances 0.000 claims description 6
- 235000003599 food sweetener Nutrition 0.000 claims description 6
- 239000003456 ion exchange resin Substances 0.000 claims description 6
- 229920003303 ion-exchange polymer Polymers 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- 235000013599 spices Nutrition 0.000 claims description 6
- 239000003381 stabilizer Substances 0.000 claims description 6
- 239000003765 sweetening agent Substances 0.000 claims description 6
- 235000002906 tartaric acid Nutrition 0.000 claims description 6
- 239000011975 tartaric acid Substances 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 5
- 102000004190 Enzymes Human genes 0.000 claims description 5
- 229940088598 enzyme Drugs 0.000 claims description 5
- 239000002304 perfume Substances 0.000 claims description 5
- 235000010378 sodium ascorbate Nutrition 0.000 claims description 5
- 229960005055 sodium ascorbate Drugs 0.000 claims description 5
- PPASLZSBLFJQEF-RKJRWTFHSA-M sodium ascorbate Substances [Na+].OC[C@@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RKJRWTFHSA-M 0.000 claims description 5
- PPASLZSBLFJQEF-RXSVEWSESA-M sodium-L-ascorbate Chemical compound [Na+].OC[C@H](O)[C@H]1OC(=O)C(O)=C1[O-] PPASLZSBLFJQEF-RXSVEWSESA-M 0.000 claims description 5
- 229960001367 tartaric acid Drugs 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 2
- 238000002791 soaking Methods 0.000 abstract description 6
- 108090000765 processed proteins & peptides Proteins 0.000 abstract description 5
- 239000007787 solid Substances 0.000 abstract description 5
- 229910052785 arsenic Inorganic materials 0.000 abstract description 4
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 abstract description 4
- 229910001385 heavy metal Inorganic materials 0.000 abstract description 4
- 239000012530 fluid Substances 0.000 abstract 2
- 108010009736 Protein Hydrolysates Proteins 0.000 abstract 1
- 238000004140 cleaning Methods 0.000 abstract 1
- 238000010612 desalination reaction Methods 0.000 abstract 1
- 230000002255 enzymatic effect Effects 0.000 abstract 1
- 238000001914 filtration Methods 0.000 abstract 1
- 238000002156 mixing Methods 0.000 abstract 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 5
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 5
- 230000004060 metabolic process Effects 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- AUNGANRZJHBGPY-SCRDCRAPSA-N Riboflavin Chemical compound OC[C@@H](O)[C@@H](O)[C@@H](O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-SCRDCRAPSA-N 0.000 description 2
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 2
- 210000004204 blood vessel Anatomy 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- GDSOZVZXVXTJMI-SNAWJCMRSA-N (e)-1-methylbut-1-ene-1,2,4-tricarboxylic acid Chemical compound OC(=O)C(/C)=C(C(O)=O)\CCC(O)=O GDSOZVZXVXTJMI-SNAWJCMRSA-N 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- AUNGANRZJHBGPY-UHFFFAOYSA-N D-Lyxoflavin Natural products OCC(O)C(O)C(O)CN1C=2C=C(C)C(C)=CC=2N=C2C1=NC(=O)NC2=O AUNGANRZJHBGPY-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 208000000501 Lipidoses Diseases 0.000 description 1
- 206010024585 Lipidosis Diseases 0.000 description 1
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 229940108928 copper Drugs 0.000 description 1
- 235000019784 crude fat Nutrition 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000004519 grease Substances 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 229960003284 iron Drugs 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 239000002417 nutraceutical Substances 0.000 description 1
- 235000021436 nutraceutical agent Nutrition 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 230000007065 protein hydrolysis Effects 0.000 description 1
- 235000019192 riboflavin Nutrition 0.000 description 1
- 229960002477 riboflavin Drugs 0.000 description 1
- 239000002151 riboflavin Substances 0.000 description 1
- 238000009366 sericulture Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 235000016804 zinc Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/78—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by ion-exchange
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/80—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter by adsorption
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/70—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter
- A23L2/84—Clarifying or fining of non-alcoholic beverages; Removing unwanted matter using microorganisms or biological material, e.g. enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
The invention provides a preparation method of a nutrient beverage containing bombyxmor protein. The preparation method comprises the following steps of firstly taking bombyxmor, thoroughly cleaning the taken bombyxmor, airing the cleaned bombyxmor, crushing the aired bombyxmor, and adding the obtained bombyxmor debris into a 1-3wt% of NaOH solution of which the weight is 4-6 times of that of the bombyxmor debris for soaking; performing filtration; then adding the pretreated debris into a 0.5-1wt% cellulase aqueous solution of which the weight is 2-4 times of that of the pretreated debris; performing enzymolysis, debitterizing enzymatic hydrolysate, and performing desalination treatment; and finally mixing bombyxmor protein fluid with 10-15wt% sodium citrate aqueous solution of which the weight is 2-2.5 times of that of the bombyxmor protein fluid so as to obtain the nutrient beverage. According to the nutrient beverage obtained by the preparation method disclosed by the invention, the peptide content is greater than or equal to 0.5%, soluble solid matters are greater than or equal to 8%, and heavy metals (lead and arsenic) are not detected. According to the nutrient beverage obtained by the preparation method disclosed by the invention, the peptide content is greater than or equal to 0.5%, the soluble solid matters are greater than or equal to 8%, and the heavy metals (lead and arsenic) are not detected.
Description
Technical field
The invention belongs to technical field of natural product extraction, and in particular to a kind of preparation of the nutritious drink containing pupa albumen
Method.
Background technology
Silkworm chrysalis（Bombyxmor i L.）For the pupa of Bombycidae silkworm.China's sericulture is with a long history, and fresh silkworm chrysalis annual production can
Up to 500,000 tons or so.Silkworm chrysalis has higher nutritive value, from ancient times just as nourishing and fit keeping function, and the food and medicine of taste.It is modern
Scientific experimentation is proved, containing abundant nutrient content in silkworm chrysalis, 25%～30% crude fat, 55%～60% thick egg is contained in dry silkworm chrysalis
In vain, 2%～4% sugar, 2%～3% chitin, 3%～5% ash content, also containing various trace elements and vitamin etc..
Pupa albumen is a kind of high-quality pure natural full-valence protein, in addition to containing the necessary amino acid of more than 60% human body, also
Rich in nutrients such as riboflavin, niacin, zinc, iron, copper, with function of physical fatigue alleviation, body metabolism can be participated in, especially
It is the metabolism of cardio-cerebrovascular, the lipidosis for being deposited on cardiovascular and cerebrovascular wall can be made to participate in metabolism again, obtains blood vessel soft
Change；And cholesterol and lipid material unnecessary in blood can be quickly reduced, and a certain degree of reduction is reached by softening blood vessel
Blood pressure is acted on, and can be widely used for preparing nutraceutical additive and nourishing agent.
Because silkworm chrysalis contains substantial amounts of grease, impact can be produced in the extraction preparation process of pupa albumen, therefore need to be right
Silkworm chrysalis carries out ungrease treatment.
The content of the invention
The technical problem of solution：The purpose of the present invention overcomes the deficiencies in the prior art and provides a kind of containing pupa albumen
The preparation method of nutritious drink, gained nutritious drink peptide content >=0.5%, soluble solid >=8%.
Technical scheme：A kind of preparation method of the nutritious drink containing pupa albumen, comprises the following steps：
Step 1, takes silkworm chrysalis, cleans, dries, and after crushing silkworm chrysalis fragment is obtained；
Step 2, silkworm chrysalis fragment is added in the 1-3wt.%NaOH solution of 4-6 times of weight and is soaked, and is filtered, and obtains pretreated broken
Piece；
Step 3, pretreated fragment is added in the 0.5-1wt.% cellulase aqueous solution of 2-4 times of weight, enzymolysis, is obtained
Enzymolysis liquid；
Step 4, after de- bitter, desalting processing is carried out to enzymolysis liquid, obtains pupa albumen liquid；
Step 5, pupa albumen liquid is mixed with the 10-15 wt.% sodium citrate aqueous solutions of 2-2.5 times of weight, is obtained final product.
Further, in step 1 silkworm chrysalis chip size in 0.5-1cm.
Further, soaking conditionses are 50-60 DEG C, 30-40min in step 2.
Further, step 3 kind enzymatic hydrolysis condition is 45-50 DEG C, 2-4h, pH8-9.
Further, suffering reason is taken off described in step 4 to be adopted containing the diatomaceous activated carbons of 10-15wt.% to enzymolysis liquid
Processed.
Further, desalting processing described in step 4 be using ion exchange resin to take off hardship after enzymolysis liquid at
Reason.
Further, sweetener, tartaric acid, ascorbic acid, anti-is also included described in step 5 in sodium citrate aqueous solution
One or several in bad hematic acid sodium, sodium acid carbonate, dextrin, spices, emulsifying agent, stabilizer.
Beneficial effect：Gained nutritious drink peptide content >=0.5% of the invention, soluble solid >=8%, heavy metal (lead,
Arsenic) do not detect.
Specific embodiment
Embodiment 1
A kind of preparation method of the nutritious drink containing pupa albumen, comprises the following steps：
Step 1, takes silkworm chrysalis, cleans, dries, and after crushing silkworm chrysalis fragment is obtained；
Step 2, silkworm chrysalis fragment is added in the 1wt.%NaOH solution of 4 times of weight and is soaked, and is filtered, and obtains pretreated fragment；
Step 3, pretreated fragment is added in the 0.5wt.% cellulase aqueous solution of 2 times of weight, enzymolysis, is digested
Liquid；
Step 4, after de- bitter, desalting processing is carried out to enzymolysis liquid, obtains pupa albumen liquid；
Step 5, pupa albumen liquid is mixed with the 10wt.% sodium citrate aqueous solutions of 2 times of weight, is obtained final product.
Wherein, in step 1 silkworm chrysalis chip size in 0.5-1cm；Soaking conditionses are 50 DEG C, 40min in step 2；Step 3 kind
Enzymatic hydrolysis condition is 45 DEG C, 4h, pH8-9；Suffering reason is taken off described in step 4 to be adopted containing the diatomaceous activated carbons of 10wt.% to enzyme
Solution liquid is processed, and the desalting processing is that the enzymolysis liquid after de- hardship is processed using ion exchange resin.Step 5 kind institute
State and also include in sodium citrate aqueous solution sweetener, tartaric acid, ascorbic acid, sodium ascorbate, sodium acid carbonate, dextrin, perfume (or spice)
One or several in material, emulsifying agent, stabilizer.
Embodiment 2
A kind of preparation method of the nutritious drink containing pupa albumen, comprises the following steps：
Step 1, takes silkworm chrysalis, cleans, dries, and after crushing silkworm chrysalis fragment is obtained；
Step 2, silkworm chrysalis fragment is added in the 2wt.%NaOH solution of 5 times of weight and is soaked, and is filtered, and obtains pretreated fragment；
Step 3, pretreated fragment is added in the 0.8wt.% cellulase aqueous solution of 3 times of weight, enzymolysis, is digested
Liquid；
Step 4, after de- bitter, desalting processing is carried out to enzymolysis liquid, obtains pupa albumen liquid；
Step 5, pupa albumen liquid is mixed with the 12wt.% sodium citrate aqueous solutions of 2 times of weight, is obtained final product.
Wherein, in step 1 silkworm chrysalis chip size in 0.5-1cm；Soaking conditionses are 55 DEG C, 35min in step 2；Step 3 kind
Enzymatic hydrolysis condition is 45 DEG C, 3h, pH8-9；Suffering reason is taken off described in step 4 to be adopted containing the diatomaceous activated carbons of 15wt.% to enzyme
Solution liquid is processed, and the desalting processing is that the enzymolysis liquid after de- hardship is processed using ion exchange resin.Step 5 kind institute
State and also include in sodium citrate aqueous solution sweetener, tartaric acid, ascorbic acid, sodium ascorbate, sodium acid carbonate, dextrin, perfume (or spice)
One or several in material, emulsifying agent, stabilizer.
Embodiment 3
A kind of preparation method of the nutritious drink containing pupa albumen, comprises the following steps：
Step 1, takes silkworm chrysalis, cleans, dries, and after crushing silkworm chrysalis fragment is obtained；
Step 2, silkworm chrysalis fragment is added in the 3wt.%NaOH solution of 6 times of weight and is soaked, and is filtered, and obtains pretreated fragment；
Step 3, pretreated fragment is added in the 1wt.% cellulase aqueous solution of 3 times of weight, enzymolysis, obtains enzymolysis liquid；
Step 4, after de- bitter, desalting processing is carried out to enzymolysis liquid, obtains pupa albumen liquid；
Step 5, pupa albumen liquid is mixed with the 12wt.% sodium citrate aqueous solutions of 3 times of weight, is obtained final product.
Wherein, in step 1 silkworm chrysalis chip size in 0.5-1cm；Soaking conditionses are 60 DEG C, 40min in step 2；Step 3 kind
Enzymatic hydrolysis condition is 50 DEG C, 4h, pH8-9；Suffering reason is taken off described in step 4 to be adopted containing the diatomaceous activated carbons of 15wt.% to enzyme
Solution liquid is processed, and the desalting processing is that the enzymolysis liquid after de- hardship is processed using ion exchange resin.Step 5 kind institute
State and also include in sodium citrate aqueous solution sweetener, tartaric acid, ascorbic acid, sodium ascorbate, sodium acid carbonate, dextrin, perfume (or spice)
One or several in material, emulsifying agent, stabilizer.
Embodiment 4
A kind of preparation method of the nutritious drink containing pupa albumen, comprises the following steps：
Step 1, takes silkworm chrysalis, cleans, dries, and after crushing silkworm chrysalis fragment is obtained；
Step 2, silkworm chrysalis fragment is added in the 3wt.%NaOH solution of 6 times of weight and is soaked, and is filtered, and obtains pretreated fragment；
Step 3, pretreated fragment is added in the 0.5wt.% cellulase aqueous solution of 4 times of weight, enzymolysis, is digested
Liquid；
Step 4, after de- bitter, desalting processing is carried out to enzymolysis liquid, obtains pupa albumen liquid；
Step 5, pupa albumen liquid is mixed with the 15wt.% sodium citrate aqueous solutions of 2.5 times of weight, is obtained final product.
Wherein, in step 1 silkworm chrysalis chip size in 0.5-1cm；Soaking conditionses are 60 DEG C, 30min in step 2；Step 3 kind
Enzymatic hydrolysis condition is 50 DEG C, 2h, pH8-9；Suffering reason is taken off described in step 4 to be adopted containing the diatomaceous activated carbons of 10wt.% to enzyme
Solution liquid is processed, and the desalting processing is that the enzymolysis liquid after de- hardship is processed using ion exchange resin.Step 5 kind institute
State and also include in sodium citrate aqueous solution sweetener, tartaric acid, ascorbic acid, sodium ascorbate, sodium acid carbonate, dextrin, perfume (or spice)
One or several in material, emulsifying agent, stabilizer.
Gained nutritious drink peptide content >=0.5% of the invention, soluble solid >=8%, heavy metal (lead, arsenic) is not detected.
Using the preparation method protein hydrolysis degree of the present invention up to 34.53%.
Claims (7)
1. a kind of preparation method of the nutritious drink containing pupa albumen, it is characterised in that：Comprise the following steps：
Step 1, takes silkworm chrysalis, cleans, dries, and after crushing silkworm chrysalis fragment is obtained；
Step 2, silkworm chrysalis fragment is added in the 1-3wt.%NaOH solution of 4-6 times of weight and is soaked, and is filtered, and obtains pretreated broken
Piece；
Step 3, pretreated fragment is added in the 0.5-1wt.% cellulase aqueous solution of 2-4 times of weight, enzymolysis, is obtained
Enzymolysis liquid；
Step 4, after de- bitter, desalting processing is carried out to enzymolysis liquid, obtains pupa albumen liquid；
Step 5, pupa albumen liquid is mixed with the 10-15 wt.% sodium citrate aqueous solutions of 2-2.5 times of weight, is obtained final product.
2. the preparation method of the nutritious drink containing pupa albumen according to claim 1, it is characterised in that：Silkworm in step 1
Pupa chip size is in 0.5-1cm.
3. the preparation method of the nutritious drink containing pupa albumen according to claim 1, it is characterised in that：Soak in step 2
Bubble condition is 50-60 DEG C, 30-40min.
4. the preparation method of the nutritious drink containing pupa albumen according to claim 1, it is characterised in that：Step 3 kind enzyme
Solution condition is 45-50 DEG C, 2-4h, pH8-9.
5. the preparation method of the nutritious drink containing pupa albumen according to claim 1, it is characterised in that：Institute in step 4
It is to adopt to process enzymolysis liquid containing the diatomaceous activated carbons of 10-15wt.% to state de- suffering reason.
6. the preparation method of the nutritious drink containing pupa albumen according to claim 1, it is characterised in that：Institute in step 4
It is that the enzymolysis liquid after de- hardship is processed using ion exchange resin to state desalting processing.
7. the preparation method of the nutritious drink containing pupa albumen according to claim 1, it is characterised in that：Institute in step 5
State and also include in sodium citrate aqueous solution sweetener, tartaric acid, ascorbic acid, sodium ascorbate, sodium acid carbonate, dextrin, perfume (or spice)
One or several in material, emulsifying agent, stabilizer.
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| CN107198252A (en) * | 2017-07-10 | 2017-09-26 | 深圳市万欣生物智能科技有限公司 | A kind of pupa albumen, its compounding albumen and preparation method thereof |
| CN108208502A (en) * | 2018-01-06 | 2018-06-29 | 佛山推启农业研究院（普通合伙） | A kind of pupa albumen nutritious drink and preparation method thereof |
| CN113424906A (en) * | 2021-07-08 | 2021-09-24 | 伊斯坎旦尔 | Silkworm chrysalis blended beverage and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107198252A (en) * | 2017-07-10 | 2017-09-26 | 深圳市万欣生物智能科技有限公司 | A kind of pupa albumen, its compounding albumen and preparation method thereof |
| CN107198252B (en) * | 2017-07-10 | 2020-10-27 | 深圳市万欣生物智能科技有限公司 | Silkworm pupa protein, compound protein thereof and preparation method thereof |
| CN108208502A (en) * | 2018-01-06 | 2018-06-29 | 佛山推启农业研究院（普通合伙） | A kind of pupa albumen nutritious drink and preparation method thereof |
| CN113424906A (en) * | 2021-07-08 | 2021-09-24 | 伊斯坎旦尔 | Silkworm chrysalis blended beverage and preparation method thereof |
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Application publication date: 20170510 |