CN106614711B - A kind of application of plant small molecular ribonucleoprotein RNA in control of insect - Google Patents
A kind of application of plant small molecular ribonucleoprotein RNA in control of insect Download PDFInfo
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- CN106614711B CN106614711B CN201610975718.1A CN201610975718A CN106614711B CN 106614711 B CN106614711 B CN 106614711B CN 201610975718 A CN201610975718 A CN 201610975718A CN 106614711 B CN106614711 B CN 106614711B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N57/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds
- A01N57/10—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds
- A01N57/16—Biocides, pest repellants or attractants, or plant growth regulators containing organic phosphorus compounds having phosphorus-to-oxygen bonds or phosphorus-to-sulfur bonds containing heterocyclic radicals
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
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- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
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Abstract
The invention discloses a kind of application of plant small molecular ribonucleoprotein RNA in control of insect, the present invention is when the difference microRNA to pest prodenia litura feed leaf mustard carries out sequencing analysis, it was found that srRNA-348-25 that expression quantity is significantly lowered simultaneously is accredited as conserved sequence generally existing in plant.Injection srRNA-348-25 segment enters polypide, and the prodenia litura death rate for feeding plant leaf blade dramatically increases.Since srRNA-348-25 is plant small molecular ribonucleoprotein RNA, more is present in rich protein-contg plant, the segment is safe to people, it can be used as the novel pesticide for safely and effectively preventing and treating phytophagous pest, it can be used directly, or production is overexpressed the genetically modified plants of srRNA-348-25.
Description
Technical field
The present invention relates to application of the plant small molecular ribonucleoprotein RNA (srRNA-348-25) in control of insect, tools
Body, which is related to plant origin srRNA-348-25, influences this function of insect growth, to turn base around srRNA-348-25 development
Cause or inhibitor research and development, achieve the purpose that control of insect.
Background technique
Since discovery small molecules interference RNA in 1993, a large amount of all kinds of microRNAs are found, include miRNAs,
SiRNAs, piRNAs and snRNAs etc..MiRNA is the microRNA of about 21~24 nucleotide non-coding of a kind of length, is deposited extensively
It is in animal and plant body.A series of experiments show miRNA may be it is a kind of it is being guarded in evolution, there is weight in organism
Want the molecule of regulating and controlling effect.MiRNA from nucleus interior coding miRNA genetic transcription at pri-microRNA, then by
Drosha enzyme is cut into the pre-miRNA that length about 70bp is in hairpin, then by caryoplasm/cytoplasm albumen from nucleus
It is inside transported in cytoplasm, is grown into the miRNA double helix complex of 18-26bp by the shearing of Dicer enzyme later.A wherein solution spiral shell
Rotation, and with AGO protein binding, and form asymmetrical RISC compound.This compound passes through miRNA therein and said target mrna
3 ' UTR complementary pairings combine, so that degrading target mRNA or blocks its translation, realize the negative regulation to target gene.?
There is lot of documents report, miRNA is played in terms of gene expression, signal transduction, development of plants, resistance stress, prevention
Vital effect.A kind of microRNA (srRNAs) from ribonucleoprotein DNA is found to have similar in recent years
The function of miRNA.Such as the microRNA high-flux sequence interpretation of result from the mankind and mouse, it is found that a large amount of srRNAs is
It can be matched with ribonucleoprotein DNA, while co-immunoprecipitation experiment shows that these srRNA can be combined with AGO；Experiment is also
Show that some srRNA may take part in relevant metabolic pathway (Haibin Wei, the Ben Zhou, Fang of regulation diabetes
Zhang,Yanyang Tu,Yanan Hu,Baoguo Zhang,Qiwei Zhai.Profiling and
Identification of Small rDNA-Derived RNAs and Their Potential Biological
Functions.PLOS ONE.2013.8(2):e56842).But the research of srRNA report is few, more not in insect and plant
Research report in terms of object relationship.
Summary of the invention
The present invention is when the difference microRNA to pest prodenia litura feed leaf mustard carries out sequencing analysis, it was found that table
Up to the srRNA-348-25:5 '-GUCGGGAGGGAAGCGGAUGGGGGCC-3 ' (SEQ ID NO:1) that significantly lowers of amount and identify
For conserved sequence generally existing in plant.Injection srRNA-348-25 segment enters polypide, feeds the twill night of plant leaf blade
The moth death rate dramatically increases.Since srRNA-348-25 is plant small molecular ribonucleoprotein RNA, more is present in rich in egg
In white plant, such as soybean, especially in the soybean of heating, therefore, which is safe to people.It can be used as safely and effectively
Prevention and treatment phytophagous pest novel pesticide, can be used directly and genetically modified plants.
The purpose of the present invention is to provide plant srRNA-348-25 to prepare the application in insecticide.
The technical solution used in the present invention is:
A kind of plant small molecular ribonucleoprotein RNA is preparing the application in insecticide, the small molecular core ribonucleoprotein
RNA is srRNA-348-25, and base sequence is as shown in SEQ ID NO:1.
Further, the insecticide can kill phytophagous polypide, or inhibit the growth of phytophagous polypide.
Further, the phytophagous polypide is Lepidoptera plant-feed insect, Homoptera plant-feed insect.
Further, the Lepidoptera and Homoptera insect are prodenia litura, silkworm, planthopper.
A kind of insecticide contains srRNA-348-25 in the insecticide, and base sequence is as shown in SEQ ID NO:1.
A kind of method of insect pest of the plant prevention and treatment, it is characterised in that: plant is made to be overexpressed small molecular core ribonucleoprotein RNA
SrRNA-348-25, srRNA-348-25 base sequence are as shown in SEQ ID NO:1.
The beneficial effects of the present invention are:
Have present invention discover that a kind of plant small molecular ribonucleoprotein RNA (srRNA-348-25) grows phytophagous pest
Have inhibiting effect, or even the death of phytophagous pest can be promoted, due to srRNA-348-25 be in mankind food plant at
Point, it to human security, can be used as the novel pesticide for safely and effectively preventing and treating phytophagous pest, can be used directly and transgenosis is planted
Object；Therefore srRNA-348-25 genetically modified plants (i.e. plant is overexpressed srRNA-348-25), which carry out control of insect, will have really
The application value on border.
Detailed description of the invention
Fig. 1 is the death condition after injecting srRNA-348-25 after Spodoptera litura larvae feed leaf mustard；NC mimic in figure
As NC (nonsense segment) control group, sr-348-25 are the abbreviation of srRNA-348-25；
Fig. 2 is the growing state of Spodoptera litura larvae after injecting srRNA-348-25 72h；Sr-348-25 is in figure
The abbreviation of srRNA-348-25；
Fig. 3 is expression of the srRNA-348-25 in different plants；Food is that prodenia litura polypide is manually trained in figure
Base is supported, it is heated to form；Bean powder, wheat bran are unheated material in figure；Leaf mustard is its blade；
The relative amount detection of srRNA-348-25 in Fig. 4 different material；
Fig. 5 is the inspection that prodenia litura feeds leaf mustard (B.juncea) and culture medium (CK) middle intestines srRNA-348-25 content afterwards
It surveys；
Fig. 6 is that detoxication enzyme SlGSTe1 expression is lowered after injection srRNA-348-25 enters Spodoptera litura larvae, and NC is in figure
Inject the control group of nonsense sequence；
Fig. 7 is the comparison of the prodenia litura growth of culture medium and leaf mustard of the feeding containing srRNA-348-25 respectively；
Fig. 8 is the comparative analysis of srRNA-348-25 sequence in various plants genome；
Fig. 9 is the silkworm phenotype for injecting 2 μ g srRNA-348-25 and nonsense sequence (NC) respectively；
Figure 10 is the death rate that srRNA-348-25 increases planthopper.
Specific embodiment
The present invention is further illustrated combined with specific embodiments below, and however, it is not limited to this.
Research process of the invention is as described below:
In order to study the mechanism that omnivorousness agricultural pests prodenia litura adapts to plant, we contain soybean and wheat to feed respectively
Intestines in the artificial medium of bran and the prodenia litura of mustard leaf have carried out high-throughput microRNA sequencing and analysis, discovery
Intestines differential expression maximum microRNA in prodenia litura, further study show that the microRNA not with prodenia litura base
It because of a group sequence pairing, but deposits in the plant genome, is a Duan Xulie of 26S rRNA, belongs to small molecular core ribonucleoprotein
RNA is named as srRNA-348-25.
SrRNA-348-25 content in the high culture medium of protein content is high, and content is very low in leaf mustard leaf；And it
Content in heating culture medium, which is higher than, does not heat culture medium, illustrates that it may be the catabolite of protein complexes.Therefore
SrRNA-348-25 content is extremely low in intestines in feeding the prodenia litura after leaf mustard, but if it is intracorporal in prodenia litura to increase it
Content, prodenia litura feed leaf mustard leaf will lead to death.
The gene expression profile in middle intestines after analyzing prodenia litura feed culture medium and leaf mustard, finds prodenia litura with albumen
Hydrolase copes with the high protein of soybean, with the plant secondary substance of detoxication enzyme reply toxicity.Analyze the target base of srRNA-348-25
Cause finds that it may inhibit detoxifying gene, without action protein hydrolase.Therefore, prodenia litura feeds high-content srRNA-
The culture medium of 348-25 is not dead, but the death rate is big after the prodenia litura feed leaf mustard of the srRNA-348-25 containing high dose in vivo
Increase.So preventing and treating prodenia litura using srRNA-348-25.
Since content is extremely low in leaves of plants by srRNA-348-25, then whether srRNA-348-25 can be used for preventing and treating
Feed other plant-feed insects? we inject srRNA-348-25 respectively and enter the silkworm of Lepidoptera and the planthopper of Homoptera,
The death rate increases after they feed plant.Since srRNA-348-25 is the ingredient in mankind food plant, to human security, because
This srRNA-348-25 genetically modified plants, which carries out control of insect, will actual application value.
Embodiment 1srRNA-348-25 inhibits the growth and survival after prodenia litura feed leaf mustard
Experimental method:
In the same size, health status first day consistent five age Spodoptera litura larvae is chosen, the weight of every cephalont is weighed,
It is randomly divided into srRNA-348-25 processing group and NC control group (nonsense segment), every group 40, repeats experiment 3 times.It is followed along blood
It is injected respectively with micro syringe between the first and second abdominal foot of flank portion of Spodoptera litura larvae ring flow direction
SrRNA-348-25 and NC (nonsense segment), injection volume are every 4 μ g of cephalont, feeding leaf mustard after injection.It sees within the 1st, 2,3 day after injection
It examines the metamorphosis of insect and evidence obtaining of taking pictures, counts the death rate of insect daily.
Above-mentioned srRNA-348-25 sequence are as follows: 5 '-GUCGGGAGGGAAGCGGAUGGGGGCC-3 ' (SEQ ID NO:1).
Experimental result:
Laboratory test results are as depicted in figs. 1 and 2, and Spodoptera litura larvae feeds mustard after Fig. 1 is injection srRNA-348-25
Death condition after dish；It can be seen that injection srRNA-348-25 Spodoptera litura larvae feed leaf mustard for 24 hours, 48h and
The death rate after 72h is respectively up to 77.50%, 92.45% and 92.45%, significantly larger than 6.91%, the 8.69% of control group (NC)
With 8.69%.Fig. 2 is the life for injecting the Spodoptera litura larvae of srRNA-348-25 or nonsense segment (NC) after feeding leaf mustard 72h
Long situation, it can be seen that the larva slight of stature survived in srRNA-348-25 processing group, and the larva of NC control group is obvious
It is loose many.
The above results illustrate that srRNA-348-25 inhibits the growth and survival after prodenia litura feed leaf mustard.
The source of embodiment 2srRNA-348-25 and its influence to prodenia litura polypide
In order to study the mechanism that microRNA regulation omnivorousness agricultural pests prodenia litura adapts to plant, respectively to feed
Artificial medium containing soybean and wheat bran and intestines in the prodenia litura of mustard leaf have carried out high-throughput microRNA sequencing
With analysis, it was found that the maximum microRNA of intestines differential expression in prodenia litura, further study show that the small molecule
RNA is not matched with prodenia litura genome sequence, but is deposited in the plant genome, is a Duan Xulie of 26S rRNA, is belonged to
Small molecular core ribonucleoprotein RNA, is named as srRNA-348-25.
One, expression of the srRNA-348-25 in different plants
Experimental method:
Respectively by prodenia litura polypide artificial medium (100g containing soybean powder, wheat bran 80g, yeast powder in every liter of culture medium
26g, casein 8g, vitamin C 8g, choline chloride 1g, sorbic acid 2g, cholesterol 0.2g, inositol 0.2g and agar powder 26g),
Bean powder, wheat bran, mustard leaf press Trizol method extracted total RNA, take 2 μ g total serum IgEs to carry out reverse transcription (reference after measuring concentration
The One Step PrimeScript miRNA cDNA Synthesis Kit specification of TAKARA carries out), obtained cDNA is produced
The template of qRT-PCR, expression water of the further qRT-PCR detection srRNA-348-25 in each sample are used for after object dilution
It is flat.Interpretation of result determines that target gene is opposite with respect to internal standard gene (GAPDH, HQ012003) using the method for relative quantification
Express multiple.Using SPSS16.0 statistical analysis software, using ANOVA (comparing between multiple processing two-by-two) or independent sample T
It examines (two sample rooms compare) and is analyzed come the comparison in difference between being handled.
Experimental result:
Testing result as shown in figure 3, there it can be seen that the srRNA-348-25 content in artificial medium (food) most
Height, the srRNA-348-25 content in bean powder are also apparently higher than wheat bran, show that the high-content srRNA-348-25 in culture medium can
It can be mainly caused by bean powder；However the srRNA-348-25 content in mustard leaf is very low.
Experimental method:
In order to further clarify bean powder heating after srRNA-348-25 content may will increase, take respectively 0.1g bean powder,
0.1g wheat bran, 0.1g (are wherein respectively provided with unheated and added by culture medium by the mixture of the 1:1 bean powder mixed and wheat bran
Hot mode treated bean powder and wheat bran mixture, 0.1g rice leaf, 0.1g peanut leaf, 0.1g yeast, carry out with it is above-mentioned
The operations such as identical RNA extraction, reverse transcription and PCR, respectively take 1 μ g RNA to carry out the PCR of reverse transcription and same volume respectively.
Experimental result:
Testing result is as shown in figure 4, there it can be seen that the expression quantity of the srRNA-348-25 in bean powder is much higher than in wheat
In bran, peanut and rice leaf；SrRNA-348-25 expression quantity does not heat relatively in bean powder and wheat bran mixture after heating
Obviously increase, show in bean powder albumen heating after, compared with polyribosome RNA generate microRNA.
The above results prompt srRNA-348-25 content in plant leaf blade very low；In the high culture medium of protein content
Content is high, and the content in culture medium after the heating further illustrates that srRNA-348-25 can higher than culture medium is not heated
It can be the catabolite of protein complexes.
Two, the relative amount of srRNA-348-25 detects after prodenia litura feed leaf mustard
The prodenia litura in second day five age is divided into 2 groups, every group of difference feeding mustard leaf (B.juncea) and manually training
It supports base (CK), the 12h and 48h after feeding, detects the content situation of srRNA-348-25 in intestines in prodenia litura.
Testing result is as shown in figure 5, there it can be seen that 12h or 48h, prodenia litura feed leaf mustard after either feeding
The content of its intracorporal srRNA-348-25 lacking compared with feed culture medium afterwards.Since srRNA-348-25 content is far high in culture medium
Content (Fig. 3) in leaf mustard leaf, thus this result shows that after prodenia litura feed in middle intestines the content of srRNA-348-25 with
Its content of srRNA-348-25 in food is directly proportional.Since insect forms not different food in long-term evolution
With adaptation mechanism, when prodenia litura feeds the culture medium containing different content srRNA-348-25 and/or leaf mustard piece respectively not
It can be dead.However ours the study found that when increase prodenia litura body in srRNA-348-25 content (hormone srRNA-
When feeding leaf mustard after 348-25) again, prodenia litura growth is suppressed and the death rate increases (see Fig. 1 and Fig. 2).
Embodiment 3srRNA-348-25 inhibits prodenia litura after feed leaf mustard to grow and promote the mechanism of its death
One, the action target of srRNA-348-25
In order to further study the mechanism that srRNA-348-25 inhibits prodenia litura growth and promotes it dead, we
Will prodenia litura feed culture medium after again rotate into food leaf mustard, then respectively rotate into food leaf mustard after 0,6,48h acquire sample into
Row high-flux sequence analyzes difference expression gene, as shown in Table 1 and Table 2.
As a result, it has been found that many proteolysis expression of enzymes of intestines lower (table 1) in prodenia litura, and many after rotating into food leaf mustard
The expression quantity for coping with the detoxication enzyme of leaf mustard secondary substance raises (table 2).And then will have in srRNA-348-25 and Tables 1 and 2
The target-gene sequence of differential expression carries out pair analysis, detects the target that srRNA-348-25 may be acted on, as a result, it has been found that
SrRNA-348-25 does not have with the proteolysis enzyme gene with differential expression that detects to be combined with possibility and (is unworthy of completely
It is right)；But specifically there is a possibility that combination with genes such as some detoxication enzymes for detecting in table 2；Further investigation revealed that
SrRNA-348-25 may (this be the weight for ensureing prodenia litura reply leaf mustard secondary substance with detoxifying gene Slgste1
Gene is wanted, it is hardly expressed when prodenia litura feeds culture medium, reference can be made to Xiaopeng Zou, Zhibin Xu,
Haiwang Zou,Jisheng Liu,Qili Feng,Sichun Zheng.Glutathione S-transferase
SlGSTE1in Spodoptera litura may be involved in feeding preference of host
Plants.Insect Biochemistry and Molecular Biology, 2016,70:32-43) 3 ' noncoding regions tool
There is combination.
1. prodenia litura of table is united from the significant proteolysis enzyme gene number of differential expression after culture medium rotates into food leaf mustard is fed
Meter
2. prodenia litura of table counts from the significant detoxifying gene number of differential expression after culture medium rotates into food leaf mustard is fed
In order to further study whether srRNA-348-25 acts on detoxifying gene Slgste1, we are by srRNA-
348-25 injection enters the Spodoptera litura larvae in five ages, detects the expression in transcriptional level of Slgste1 gene；Detection knot
Fruit as shown in fig. 6, there it can be seen that injection srRNA-348-25 after, the transcription of Slgste1 gene in Spodoptera litura larvae body
Expression is substantially reduced, and illustrates that srRNA-348-25 inhibits Slgste1 gene expression.
Two, phenotypic analysis
Experimental method:
5 groups of experiments are set altogether, and each group disposition is as follows:
Control group (simple water): the simple fed with water of prodenia litura is given, such as Fig. 7 A；
Experimental group (simple water srRNA): the simple feeding srRNA-348-25 of prodenia litura is given, compared with water, it was demonstrated that
Whether srRNA-348-25 is non-toxic, such as Fig. 7 A；
Control group (culture medium): prodenia litura is made to feed artificial medium, such as Fig. 7 B；
Experimental group (2 μ g srRNA+ culture medium): prodenia litura feed is made to contain the artificial culture of 2 μ g srRNA-348-25
Base, such as Fig. 7 B and Fig. 7 C；
Experimental group (2 μ g srRNA+ leaf mustard): prodenia litura feed is made to contain the leaf mustard of 2 μ g srRNA-348-25, such as Fig. 7 C；
According to above-mentioned processing, the growth and death condition of each group Spodoptera litura larvae are observed respectively.
Observation result is as shown in fig. 7, as can be seen that 2 simple μ g srRNA-348-25 do not influence twill night from Fig. 7 A
The growth and development of moth illustrates that srRNA-348-25 may not act on any gene relevant to growth；It can be seen that from Fig. 7 B
The prodenia litura of culture medium of the feed containing 2 μ g srRNA-348-25 is compared with the prodenia litura of feed media alone, and growth is simultaneously
It is not suppressed, shows that srRNA-348-25 does not regulate and control the table of gene such as proteolytic enzyme relevant to medium component yet
It reaches；As can be seen that the prodenia litura of culture medium and leaf mustard of the feed containing 2 μ g srRNA-348-25, the growth of the latter from Fig. 7 C
It is significantly less than the former, illustrates that the growth of prodenia litura can be inhibited by eating leaf mustard again when polypide is enriched with 2 μ g srRNA-348-25,
Promote the death of polypide, mechanism of action therein is likely due to srRNA-348-25 and inhibits the detoxication enzymes base such as Slgste1
Because of the expression of (polypide decomposes enzyme necessary to poisonous plants secondary substance), increases the toxicity of leaf mustard, eventually lead to polypide
Feed and growth retardation, to promote the death of polypide.
The comparative analysis of srRNA-348-25 sequence in the different Plant Genomes of embodiment 4
Since srRNA-348-25 sequence can not find the sequence of pairing in prodenia litura genome, we distinguish
Have chosen several Plant Genomes and compare analysis, include soybean (GenBank sequences number: dbj | LB172039.1), on
Sheep's hay (GenBank sequences number: gb | AOCO010307372.1)；Arabidopsis (gene group #: TAIRIO).Comparison result such as Fig. 8
Shown, it is a Duan Xulie of 26s rRNA, this explains why it is in high egg that srRNA-348-25 is very conservative in plant
Content is higher in white soybean, and content is all low in the blade of all kinds of plants.Therefore, theoretically transgenosis srRNA-348-
25, increase its content of the blade of all kinds of plants, be likely to arrive cause to feed such plant pest generate it is undesirable anti-
It answers.
The growth of embodiment 5srRNA-348-25 inhibition silkworm
Since content is extremely low in leaves of plants by srRNA-348-25, then whether srRNA-348-25 can be used for preventing and treating
Feed other plant-feed insects? the present embodiment has carried out corresponding research to the silkworm of Lepidoptera.
After having injected whether also the silkworm in 3 days 5 ages is taken respectively, injects 2 μ g srRNA-348-25 and nonsense control sequence,
Mulberry leaf are fed to silkworm, observe the growing state of silkworm.
Result is observed as shown in figure 9, there it can be seen that injecting after srRNA-348-25 72 days, the growing state of silkworm
It is obviously suppressed, growth inhibition ratio 100%.
The death of embodiment 6srRNA-348-25 promotion planthopper
Since content is extremely low in leaves of plants by srRNA-348-25, then whether srRNA-348-25 can be used for preventing and treating
Feed other plant-feed insects? the present embodiment injects 0.2 μ g srRNA- to the planthopper of the primary pest Homoptera of rice respectively
348-25 and nonsense sequence (control group), volume injected are 0.2 μ l, whether also to feed Rice Leaf to planthopper after having injected
(planthopper is put into rice after having injected) observes the growing state of planthopper.
The results are shown in Figure 10 for observation, there it can be seen that 2 days after injection srRNA-348-25, srRNA-348-25's
The death rate is 86%, and the death rate of control group is only 33%.
The above results illustrate the content for increasing srRNA-348-25 in planthopper body, and planthopper can also be promoted to feed Rice Leaf
The death rate afterwards further proves that srRNA-348-25 can promote the death rate of the insect of phytophagous.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
SEQUENCE LISTING
<110>South China Normal University
<120>application of a kind of plant small molecular ribonucleoprotein RNA in control of insect
<130>
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 25
<212> RNA
<213>artificial sequence
<400> 1
gucgggaggg aagcggaugg gggcc 25
Claims (6)
1. a kind of plant small molecular ribonucleoprotein RNA is preparing the application in insecticide, the small molecular core ribonucleoprotein RNA
For srRNA-348-25, base sequence is as shown in SEQ ID NO:1.
2. application according to claim 1, it is characterised in that: the insecticide can kill phytophagous polypide, or inhibit
The growth of phytophagous polypide.
3. application according to claim 2, it is characterised in that: the phytophagous polypide is Lepidoptera plant-feed insect, together
Wing mesh plant-feed insect.
4. application according to claim 3, it is characterised in that: the Lepidoptera and Homoptera insect be prodenia litura, silkworm,
Planthopper.
5. a kind of insecticide, it is characterised in that: contain srRNA-348-25, base sequence such as SEQ ID NO in the insecticide:
Shown in 1.
6. a kind of method of insect pest of the plant prevention and treatment, it is characterised in that: plant is made to be overexpressed small molecular core ribonucleoprotein RNA
SrRNA-348-25, srRNA-348-25 base sequence are as shown in SEQ ID NO:1.
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| CN101870979A (en) * | 2010-06-02 | 2010-10-27 | 中国农业科学院植物保护研究所 | Insecticidal gene cryX with high toxicity to lepidopterous pests and application thereof |
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| CN105165885A (en) * | 2015-09-16 | 2015-12-23 | 华南师范大学 | Application of miR-375-3p in prevention and treatment of lepidoptera pests |
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