CN106608837A - Method for extracting gamma-aminobutyric acid from mulberry leaves - Google Patents
Method for extracting gamma-aminobutyric acid from mulberry leaves Download PDFInfo
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- CN106608837A CN106608837A CN201510686900.0A CN201510686900A CN106608837A CN 106608837 A CN106608837 A CN 106608837A CN 201510686900 A CN201510686900 A CN 201510686900A CN 106608837 A CN106608837 A CN 106608837A
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- aminobutyric acid
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Abstract
The invention discloses a method for extracting gamma-aminobutyric acid from mulberry leaves. The method comprises the following five process steps: withering; anaerobic treatment; extraction; concentration; and drying. The method is characterized in that a part of moisture in fresh mulberry leaves is removed through withering; repeated anaerobic treatment is carried out for enrichment gamma-aminobutyric acid; deionized water is used for solvent extraction of soluble substances in the mulberry leaves so as to obtain extract; the extract is concentrated so as to obtain a concentrate; and the concentrate is subjected to refrigeration, sublimation and drying so as to obtain gamma-aminobutyric acid. The method provided by the invention has the characteristics of simple process steps and high extraction rate and high purity of gamma-aminobutyric acid. The gamma-aminobutyric acid obtained in the invention is capable of adjusting blood pressure and heart rate, nourishing nerve cells, promoting mental tranquilization and blood circulation in the brain, improving cerebral vitality, lowering blood pressure, enhancing liver functions, promoting alcohol metabolism, eliminating odor, etc.
Description
Technical field
The invention belongs to food processing technology field, and in particular to one kind extracts γ-aminobutyric acid preparation method from Folium Mori.
Background technology
γ-aminobutyric acid is a kind of feature acidic amino acid of nonprotein, it is widely present in nature, it is the important inhibitory neurotransmitter in mammalian central nervous system, with important body function, there are regulation blood pressure and heart rate, trophic nerve cell, promote ataraxy, promote brain blood flow, promote the multiple efficacies such as brain vigor, GABA content in natural food materials is mainly improved by concentration method, add Glu or using the Glu contained in itself in food materials using external source, the GAD enzymes that activation contains in itself, it is enriched with, current main brown rice, Folium Mori etc. are enriched with, because Glu is limited in raw material, the simple Glu using raw material itself, there is concentration of substrate to be limited, GAD enzymes are using incomplete, the problems such as product GABA content is not high.External source addition Glu can increase substantially product G ABA content.
With the further investigation of GABA, GABA is widely used in the industries such as medicine, food, health care, chemical industry and agricultural by as a kind of new function sex factor.China's Morus alba Resource Development is enriched, and research finds the GABA containing high-load in Folium Mori, and GABA average contents are 226mg in 100g Folium Mori dry products, so γ-aminobutyric acid is extracted from Folium Mori being increasingly subject to people's attention, wide market.The Chinese patent literature of Application No. 200910040513.4 discloses mulberry leaf gamma-aminobutyric acid extracting method and its utilization；With fresh mulberry leaf as raw material, drying is crushed, and is soaked, ultrasonic extraction, and dehydrated alcohol precipitation, three-level the operation such as isolates and purifies and γ-aminobutyric acid white crystal is obtained；But the method has production cost height, the utilization rate of Folium Mori is low.
The content of the invention
The invention provides it is a kind of from Folium Mori extract γ-aminobutyric acid method, overcome above-mentioned prior art no
Foot, its existing γ-aminobutyric acid extracting method of energy effectively solving has that high and Folium Mori the utilization rate of production cost is low.
To achieve these goals, the present invention proposes following technical scheme：
One kind extracts γ-aminobutyric acid preparation method from Folium Mori, and the method is comprised the following steps：
(1) wither：It is 50-70% that fresh mulberry leaf withers to water content；
(2) anaerobism anaerobic treatment：Fresh leaf vacuum nitrogen after withering processes 3h process, then room temperature spreading for cooling Aerobic Process for Treatment 2h,
So processing 2-3 time repeatedly carries out γ-aminobutyric acid enrichment；
(3) extract：By the fresh mulberry leaf after enrichment, deionized water is added, blended, extract 15-30min, clarify 30-50min, be centrifuged under the conditions of 7000rpm, obtain clarification Mori folium extract；
（4）Concentration：Extracting solution is concentrated, concentrated solution is obtained；
（5）By concentrated solution drying bu sublimation, γ-aminobutyric acid is obtained.
Preferably, the step（3）Described in add deionized water and the fresh mulberry leaf mass volume ratio be 1:1.
Preferably, the step (3) is that Folium Mori are placed in into the soluble substance that 15-30min in deionized water extracts Folium Mori, obtains extracting solution.
Preferably, the step (4) is less than 55 DEG C by extracting solution more than 0.1MPa vacuums, is concentrated into 15-20 Baume degrees, obtains first time concentrated solution, then by first time concentrated solution through a semipermeable membrane, obtains second concentrated solution.
Preferably, it by second concentrated solution in step (4) in temperature is -30 DEG C that the step (5) is, vacuum is to be freezed under the conditions of 0.1～0.06 millimetres of mercury, then carries out ice crystal distillation, finally obtains γ-aminobutyric acid.
A kind of method that γ-aminobutyric acid is extracted from Folium Mori provided by the present invention, make in Folium Mori rich in GABA, become a kind of with the γ-aminobutyric acid for adjusting blood pressure and heart rate, trophic nerve cell, promote ataraxy, promote brain blood flow, promote the multiple efficacies such as brain vigor.
Compared with prior art, it is also manifested by using beneficial effects of the present invention：
First, processing step is simple, and Folium Mori are through step（3）The extracting solution obtained after extraction, extraction ratio is high；
2nd, extracting solution is through step（4）After concentrating twice, the concentrated solution liquid for obtaining is limpid bright, without precipitation and research of chaotic phenomenon；
3rd, second concentrated solution is through step（5）After drying bu sublimation, the γ-aminobutyric acid for obtaining has the characteristics of solubility is good, purity is high.
The additional aspect and advantage of the present invention will be set forth in part in the description, and partly will become apparent from the description below, or be recognized by the practice of the present invention.
Specific embodiment
Technical scheme is described further below by embodiment, but is not considered as limiting the invention.
Embodiment 1
It is 50-70% to take 5000 grams of fresh mulberry leaf and wither to water content, fresh leaf vacuum nitrogen after withering processes 3h process, then room temperature spreading for cooling Aerobic Process for Treatment 2h, so processing 2 times repeatedly carries out γ-aminobutyric acid enrichment, and GABA content reaches 2.52mg/g in dry Folium Mori after enrichment；Fresh mulberry leaf after being enriched with, adds 5000ml deionized waters, blends, stirring and leaching 20min, stands clarification 30min, is centrifuged under the conditions of 7000rpm, must clarify extracting solution 4000ml.By extracting solution more than 0.1MPa vacuums, temperature obtains first time concentrated solution under the conditions of 50 DEG C, to be concentrated into 18 Baume degrees, then by first time concentrated solution through a semipermeable membrane, obtains second concentrated solution.In temperature it is -30 DEG C by second concentrated solution, vacuum is to be freezed under the conditions of 0.09 millimetres of mercury, then carries out ice crystal distillation, and the γ-aminobutyric acid of 2.95g or so is finally obtained, and 100g fresh mulberry leafs extract 59.00mg γ-aminobutyric acid.
γ-aminobutyric acid that the present invention is obtained is faint yellow or the purity of white powder γ-aminobutyric acid after testing averagely reaches 90%, and the existing mulberry leaf gamma-aminobutyric acid extracting method and its content of γ-aminobutyric acid is suitable in γ-aminobutyric acid white crystalline body in using.
Embodiment 2
It is 60-70% to take fresh mulberry leaf 40Kg and wither to water content, and the fresh leaf vacuum nitrogen after withering processes 3h process, then room temperature spreading for cooling Aerobic Process for Treatment 2h, and so processing 2 times repeatedly carries out γ-aminobutyric acid enrichment, and GABA content reaches 2.48mg/g in dry Folium Mori after enrichment；Fresh mulberry leaf after being enriched with, adds 40L deionized waters, blends, stirring and leaching 30min, stands clarification 30min, is centrifuged under the conditions of 7000rpm, must clarify extracting solution 33L.By extracting solution more than 0.1MPa vacuums, temperature obtains first time concentrated solution under the conditions of 45 DEG C, to be concentrated into 15 Baume degrees, then by first time concentrated solution through a semipermeable membrane, obtains second concentrated solution.In temperature it is -30 DEG C by second concentrated solution, vacuum is to be freezed under the conditions of 0.09 millimetres of mercury, then carries out ice crystal distillation, and the γ-aminobutyric acid of 23.12g or so is finally obtained, and 100g fresh mulberry leafs extract 57.80mg γ-aminobutyric acid.
γ-aminobutyric acid that the present invention is obtained is faint yellow or the purity of white powder γ-aminobutyric acid after testing averagely reaches 90%, and the existing mulberry leaf gamma-aminobutyric acid extracting method and its content of γ-aminobutyric acid is suitable in γ-aminobutyric acid white crystalline body in using.
The invention is not restricted to above example, but all should fall within the claims in the present invention protection domain.
Claims (5)
1. it is a kind of from Folium Mori extract γ-aminobutyric acid method, it is characterised in that the method is comprised the following steps：
(1) wither：It is 50-70% that fresh mulberry leaf withers to water content；
(2) anaerobism anaerobic treatment：Fresh leaf vacuum nitrogen after withering processes 3h process, then room temperature spreading for cooling Aerobic Process for Treatment 2h, and so processing 2-3 time repeatedly carries out γ-aminobutyric acid enrichment；
(3) extract：By the fresh mulberry leaf after enrichment, deionized water is added, blended, extract 15-30min, clarify 30-50min, be centrifuged under the conditions of 7000rpm, obtain clarification Mori folium extract；
(4) concentrate：Extracting solution is concentrated, concentrated solution is obtained；
(5) it is dried：By concentrated solution drying bu sublimation, γ-aminobutyric acid is obtained.
2. according to claim 1 it is a kind of from Folium Mori extract γ-aminobutyric acid method, it is characterised in that the step（3）Described in add deionized water and the fresh mulberry leaf mass volume ratio be 1:1.
3. a kind of method that γ-aminobutyric acid is extracted from Folium Mori according to claim 1, it is characterised in that the step (3) is that Folium Mori are placed in into the soluble substance that 15-30min in deionized water extracts Folium Mori, obtains extracting solution.
4. according to claim 1 it is a kind of from Folium Mori extract γ-aminobutyric acid method, it is characterized in that, the step (4) be by extracting solution more than 0.1MPa vacuums, less than 55 DEG C, it is concentrated into 15-20 Baume degrees, first time concentrated solution is obtained, then by first time concentrated solution through a semipermeable membrane, obtains second concentrated solution.
5. according to claim 1 it is a kind of from Folium Mori extract γ-aminobutyric acid method, it is characterized in that, the step (5) be by second concentrated solution in step (4) temperature be -30 DEG C, vacuum is to be freezed under the conditions of 0.1～0.06 millimetres of mercury, ice crystal distillation is carried out again, finally obtains γ-aminobutyric acid.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115477592A (en) * | 2022-09-30 | 2022-12-16 | 西安萃源生物科技有限公司 | Crystallization method of gamma-aminobutyric acid |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115477592A (en) * | 2022-09-30 | 2022-12-16 | 西安萃源生物科技有限公司 | Crystallization method of gamma-aminobutyric acid |
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