CN106596912A - Method for diagnosing ketosis of cow after delivery by use of citric acid in milk - Google Patents
Method for diagnosing ketosis of cow after delivery by use of citric acid in milk Download PDFInfo
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- CN106596912A CN106596912A CN201611221630.7A CN201611221630A CN106596912A CN 106596912 A CN106596912 A CN 106596912A CN 201611221630 A CN201611221630 A CN 201611221630A CN 106596912 A CN106596912 A CN 106596912A
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- citric acid
- milk
- ketoacidosis
- cow
- bhba
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/02—Food
- G01N33/04—Dairy products
- G01N33/06—Determining fat content, e.g. by butyrometer
Abstract
The invention provides a method for diagnosing ketosis of cow after delivery by use of citric acid in milk. The citric acid in milk is very sensitive to ketone body change when the cow suffers from ketosis, the cow ketosis is diagnosed by detecting the content of citric acid in the milk, the relationship between an energy metabolism characteristic index and the citric acid in the milk is illustrated, the citric acid in the milk has significant positive correlation with BHBA and NEFA which are important detection indexes of the cow ketosis, the citric acid in the milk can serve as an in-vitro detection index for monitoring and diagnosing the cow ketosis, and when the content of the citric acid in the milk is higher than 0.205%, the cow can be diagnosed with ketosis.
Description
Technical field
The present invention relates to a kind of method of ketoacidosis in diagnosis of milk cow puerperal, particularly a kind of using citric acid diagnosis of milk cow in breast
Puerperal ketoacidosis method.
Background technology
With greatly developing for global animal husbandry, dairy has become an important industry for supporting animal husbandry development.
Ketosis is a kind of modal nutrition metabolic diseases of perinatal stage, and especially high yield cow is multiple, there is prevalence all over the world, although
Can seldom cause death, but milk production of cow can be caused to reduce, increase medical expense, while the milk constituents of ketoacidosis milch cow output
Can change, harmful effect may be produced to human body, to the large-scale cattle farm of many high yields serious economic loss, institute are brought
Increasingly paid attention to by culturists with Ketosis.
Citric acid, also known as citric acid, 2- hydroxy propane tricarboxylic acids, by S-acetyl-coenzyme-A and oxalyl in body tricarboxylic acid cycle
Acetic acid carboxylic is closed and formed, and participates in sugar, fat and protein metabolism in vivo.There are some researches show when milch cow occurs ketoacidosis, ill milk
The milk constituents of cattle output can change, and forefathers carry out nuclear magnetic resonance, NMR to healthy cow with ketoacidosis milk Ox blood plasma is suffered from（1H
Nuclear Magnetic Resonance, 1H NMR）, analysis finds that the citric acid in ketoacidosis milk Ox blood plasma can occur significantly change
Change, at the same time it has been proposed that when milch cow occurs ketoacidosis, the citric acid in breast also can change.
The monitoring of ketoacidosis at present is mainly according to beta-hydroxybutyric acid in blood（BHBA）Concentration change, works as BHBA>1.2mmol/L
When it is diagnosable be that milch cow suffers from ketoacidosis, although this kind of method accuracy rate is high, its testing cost is big, easily by extraneous factor shadow
Ring, need collection blood to be detected；Collection blood can cause irritable injury to milch cow.Therefore need to find sensitivity
A kind of method of ketoacidosis in diagnosis of milk cow puerperal high, to milch cow fanout free region.
The content of the invention
The present invention provides a kind of method using citric acid diagnosis of milk cow ketoacidosis in puerperal in breast, and citric acid is to milk in the breast
Ketoboidies changes more sensitive during cattle ketoacidosis, by citric acid content in detection breast come diagnosis of milk cow ketoacidosis, illustrates energy metabolism special
Relation in levying property index BHBA and breast between citric acid, shows that citric acid and Ketosis are in extremely notable positive correlation in breast.
The technical solution adopted in the present invention is：A kind of method using citric acid diagnosis of milk cow ketoacidosis in puerperal in breast, breast
Middle citric acid is closely related with Ketosis, and using analysis of milk composition instrument and automatic clinical chemistry analyzer Fructus Citri Limoniae in Lac Bovis seu Bubali is determined
Acid, butterfat；The content of BHBA, NEFA in serum, is in extremely to show by citric acid in statistical analysis breast and BHBA, NEFA, butterfat
Write positive correlation（P<0.01）, ketoacidosis can be suffered from diagnosis of milk cow when citric acid is more than 0.205% in breast.
Laboratory animal quantity, Testing index citric acid, BHBA, NEFA, butterfat are respectively provided with statistical significance in such scheme；
Person correlation analysiss discoveries are carried out to experimental data, citric acid is in extremely significant with BHBA, NEFA, dairy fat content in breast
Positive correlation（P<0.01）, correlation coefficient is respectively r=0.450, p=0.000；R=0.504, p=0.000；R=0.315, p=0.006.
Independent sample T inspections are carried out with health group experimental data to ketoacidosis group, as a result shows ketoacidosis group BHBA, NEFA, citric acid pole
Significantly higher than healthy group（P<0.01）.ROC curve early warning analysis is carried out to two groups of experimental datas, area under curve is 0.755,
Citric acid is 0.205% to the early warning value of ketoacidosis, when citric acid is more than 0.205% in breast, can suffer from ketoacidosis with diagnosis of milk cow.
The invention has the advantages that：The present invention is based on above-mentioned background by ketoacidosis milch cow and healthy cow milk
BHBA, NEFA content carries out Person correlation analysiss and ROC curve analysis in middle citric acid content, blood, specifies milch cow and sends out
Citric acid Changing Pattern in milk during raw ketoacidosis, determines citric acid and specific biochemical index during milch cow generation ketoacidosis in milk
BHBA, NEFA are in extremely notable positive correlation（P<0.01）, citric acid can be come more with a kind of index of vitro detection ketoacidosis in milk
Accurate more easily monitoring, diagnosis of milk cow ketoacidosis, milch cow is can determine when citric acid is more than 0.205% in breast with ketoacidosis,
The method can avoid the injury caused to milch cow of taking a blood sample, and detect ketoacidosis earlier, can reduce the damage of milk cow production
Lose.
Description of the drawings
Fig. 1 is BHBA and citric acid correlative trend figure
Fig. 2 is NEFA and citric acid correlative trend figure
Fig. 3 is butterfat and citric acid correlative trend figure
Fig. 4 is the early warning curve chart of citric acid ROC curve
Specific embodiment
With reference to experiment, the present invention is further described：
Zoopery：In Heilongjiang Province Mishan City scattered bar raise, the scale cattle farm of TMR feedings, choose the age, body condition,
Parity it is close puerperal 15～60d milch cow 74.Serum and milk that collection same time is selected, use entirely certainly gathering serum
Automatic Biochemical Analyzer detects BHBA, NEFA content, using BHBA as diagnosis of milk cow ketoacidosis standard；BHBA in cow serum>
1.2mmol/L, is diagnosed as ketoacidosis group;BHBA<1.2mmol/L and no obvious clinical symptoms, are defined as healthy group.Its
Middle health group 41, ketoacidosis group 33.Additionally, mastitis are wherein suffered from, and metritiss, miscarriage, due to drug induced hepatopathy,
The milch cow of the diseases such as heating is excluded this test.
Sample collecting and process：All milch cows mL of tail vein blood 10 before early morning feeding, after natural coagulation, room
10 min are centrifuged with the rotating speed of 3000 rpm under temperature, the i.e. required serum of supernatant is drawn in EP pipes, frozen in -20 DEG C of refrigerators
It is to be checked.
Index determining method：Citric acid is detected with dairy fat content using analysis of milk composition instrument in breast, BHBA, NEFA generation
Thank to index to be measured using automatic clinical chemistry analyzer.
Data analysiss：All market demand IBM SPSS19.0 softwares carry out statistical analysiss, measurement data mean with x ±
S represents that the average significance of difference adopts independent sample T check analyses between group；Correlation analysiss adopt Pearson linear correlations
Property analysis,P<0.01For extremely significantly correlated；Forewarning index value is calculated to be analyzed using ROC curve, and area under curve is represented more than 0.5
Index has early warning meaning, represents there is general forewarning function between 0.7～0.9, can enter as golden index more than 0.9
Row diagnosis.
Experimental result：
BHBA, NEFA testing result is shown in Table 1 in citric acid, butterfat and blood in the breast of 74 milch cows of statistics.
The citric acid of table 1, butterfat, BHBA, NEFA Testing index situation
| Project | Detected value |
| NEFA (mmol/L) | 0.76±0.37 |
| BHBA (mmol/L) | 1.47±1.05 |
| Butterfat % | 4.01±1.39 |
| Citric acid % | 0.20±0.04 |
Using independent sample T check analyses methods, analysis is compared to two groups of experiment milch cow difference Testing index.Two groups of milch cows
Citric acid, BHBA, NEFA, the comparative analysiss of butterfat index the results are shown in Table 2.In ketoacidosis group milk Lac Bovis seu Bubali in citric acid and serum
BHBA, NEFA all pole is significantly higher than healthy group（P<0.01）.
2 two groups of milch cow citric acids of table, BHBA, NEFA, the comparison of butterfat index
| NEFA | BHBA | Butterfat | Citric acid | |
| Healthy group（41） | 0.56±0.23** | 0.74±0.19** | 4.02±1.60 | 0.18±0.04** |
| Ketoacidosis group（33） | 1.02±0.34** | 2.38±0.97** | 4.00±1.10 | 0.22±0.04** |
Citric acid the results are shown in Table 3 with the correlation analysiss of ketoacidosis Metabolic indices in Person correlation analysiss breast.Fructus Citri Limoniae in breast
Acid is in extremely notable positive correlation with BHBA, NEFA, butterfat（P<0.01）.
The citric acid of table 3 and BHBA, NEFA, the index related analysis of butterfat
By BHBA, NEFA, butterfat and citric acid testing result draw correlative trend figure as shown in Figure 1, Figure 2, Fig. 3；BHBA in blood
It is proportionate trend with citric acid in NEFA and milk, the butterfat percnetage in milk and citric acid in breast are also proportionate trend.
Using the ROC curve in SPPA softwares, forewarning function of the citric acid to Ketosis in analysis breast, curve chart is such as
Figure, is shown in Figure of description（Fig. 4）It is shown, as a result show that area under curve is 0.755, illustrate that citric acid has one to Ketosis
As forewarning function, early warning value is 0.205%.
Citric acid and Ketosis important Testing index BHBA, NEFA are in extremely notable positive correlation in breast, and citric acid can in breast
Monitored using the index as vitro detection ketoacidosis, diagnosis of milk cow ketoacidosis, when in breast citric acid be more than 0.205% when can diagnose
Milch cow suffers from ketoacidosis.
Claims (2)
1. a kind of method of citric acid diagnosis of milk cow ketoacidosis in puerperal during utilization is newborn, it is characterised in that:Citric acid and milch cow ketone in breast
Disease is closely related, using ketone in citric acid content in analysis of milk composition instrument and automatic clinical chemistry analyzer measure Lac Bovis seu Bubali and serum
Disease-specific metabolic index beta-hydroxybutyric acid（BHBA）, free fatty（NEFA）Concentration, by statistical analysis citric acid and ketone
Disease is in extremely notable positive correlation（P<0.01）；Ketoacidosis can be suffered from diagnosis of milk cow when citric acid is more than 0.205% in milk.
2. the method for citric acid diagnosis of milk cow ketoacidosis in puerperal during utilization according to claim 1 is newborn, it is characterised in that:It is right
Experimental data carries out Person correlation analysiss discoveries, and citric acid and BHBA, NEFA content are in extremely significant positive correlation in breast
（P<0.01）, correlation coefficient is respectively r=0.450, p=0.000；R=0.504, p=0.000；To ketoacidosis group and health group experiment number
According to independent sample T inspections are carried out, as a result show that ketoacidosis group BHBA, NEFA, citric acid pole is significantly higher than healthy group（P< 0.01）；ROC curve early warning analysis is carried out to two groups of experimental datas, area under curve is 0.755, early warning of the citric acid to ketoacidosis
It is worth for 0.205%.
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| CN201611221630.7A CN106596912B (en) | 2016-12-27 | 2016-12-27 | A method of utilizing citric acid diagnosis of milk cow postpartum ketoacidosis in cream |
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| CN201611221630.7A CN106596912B (en) | 2016-12-27 | 2016-12-27 | A method of utilizing citric acid diagnosis of milk cow postpartum ketoacidosis in cream |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109378069A (en) * | 2018-08-28 | 2019-02-22 | 昆明理工大学 | A kind of method for early warning based on disease symptoms correlation |
| CN113481220A (en) * | 2021-08-27 | 2021-10-08 | 黑龙江八一农垦大学 | Blood coding gene CYP1A1 related to dairy cow ketosis and PCR detection kit thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1595154A (en) * | 2004-07-14 | 2005-03-16 | 深圳华康生物医学工程有限公司 | Refined citric acid quantitative determination reagent |
| CN101118237A (en) * | 2007-09-05 | 2008-02-06 | 吉林大学 | Subclinical ketosis of milk cattle diagnose indicator paper |
| EP2762882A1 (en) * | 2013-01-31 | 2014-08-06 | Sensirion Holding AG | Portable electronic device with ketone sensor |
| US9316591B1 (en) * | 2015-02-09 | 2016-04-19 | University Of Guelph | Biosensor for detection of subclinical ketosis |
-
2016
- 2016-12-27 CN CN201611221630.7A patent/CN106596912B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1595154A (en) * | 2004-07-14 | 2005-03-16 | 深圳华康生物医学工程有限公司 | Refined citric acid quantitative determination reagent |
| CN101118237A (en) * | 2007-09-05 | 2008-02-06 | 吉林大学 | Subclinical ketosis of milk cattle diagnose indicator paper |
| EP2762882A1 (en) * | 2013-01-31 | 2014-08-06 | Sensirion Holding AG | Portable electronic device with ketone sensor |
| US9316591B1 (en) * | 2015-02-09 | 2016-04-19 | University Of Guelph | Biosensor for detection of subclinical ketosis |
Non-Patent Citations (13)
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109378069A (en) * | 2018-08-28 | 2019-02-22 | 昆明理工大学 | A kind of method for early warning based on disease symptoms correlation |
| CN113481220A (en) * | 2021-08-27 | 2021-10-08 | 黑龙江八一农垦大学 | Blood coding gene CYP1A1 related to dairy cow ketosis and PCR detection kit thereof |
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| CN106596912B (en) | 2019-01-11 |
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