CN106591192B - One plant of tricyclazole degradation sphingomonas bacteria and its application in wastewater treatment containing tricyclazole - Google Patents
One plant of tricyclazole degradation sphingomonas bacteria and its application in wastewater treatment containing tricyclazole Download PDFInfo
- Publication number
- CN106591192B CN106591192B CN201611204665.XA CN201611204665A CN106591192B CN 106591192 B CN106591192 B CN 106591192B CN 201611204665 A CN201611204665 A CN 201611204665A CN 106591192 B CN106591192 B CN 106591192B
- Authority
- CN
- China
- Prior art keywords
- tricyclazole
- degradation
- sphingomonas
- njust37
- bacteria
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/306—Pesticides
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/40—Organic compounds containing sulfur
Abstract
The invention discloses one plant of tricyclazole degradation sphingomonas bacteria and its applications in wastewater treatment containing tricyclazole.The present invention directlys adopt the enrichment that tricyclazole degradation bacteria is carried out using tricyclazole as the culture medium of sole carbon source, nitrogen source and sulphur source, and it uses and is separated using tricyclazole as sole carbon source, nitrogen source with the screening and culturing medium of sulphur source, screening obtains tricyclazole degradation bacteria, it is identified as sphingomonas bacteria, it is named as Sphingomonas sp.NJUST37, deposit number is CCTCC M 2016520.Sphingomonas bacteria Sphingomonas sp.NJUST37 of the invention can realize the degradable of the waste water containing tricyclazole containing higher concentration, and the degradable up to tricyclazole of 270mg/L, there is preferable tolerance performance to tricyclazole, have a good application prospect in wastewater treatment containing tricyclazole.
Description
Technical field
The invention belongs to environmental organic pollutant biologic treating technique fields, and in particular to one plant has tricyclazole degradation function
The sphingomonas bacteria of energy and its application in biological wastewater treatment and environmental pollution are repaired.
Background technique
Tricyclazole is a kind of systemic fungicide, it is mainly used to as protective agent, therapeutic agent and the shovel in rice cropping
Except agent, control rice blast fungus and cause rice blast, it also be used to control bunt, damping-off, striped leaf, net blotch and
Other cereal diseases.Since the rock-steady structure three aspect factor of phenyl ring spatial volume steric effect, hydrophobicity and three rings is comprehensive
Under cooperation is used, the biodegrade of tricyclazole is weaker, meanwhile, the latency environment harm of tricyclazole can be by it in water body-soil system
In stability reflect, also, can increase with number of rings purpose some researches show that the mutagenesis ability of nitrogen heterocyclic and
Increase, this can also result in the mutagenicity and toxicity of tricyclazole.And tricyclazole is as a kind of fungicide, is a kind of typical agriculture
Medicine azacyclo- organic compound, therefore, with pesticide industry produce in the discharge of waste water and the extensive application of pesticide, tricyclazole is
As residual contaminants common in soil, surface water and underground water.This causes the health and ecological environment of the mankind huge
Big potential hazard.Therefore, such as direct emission without processing of the waste water containing tricyclazole can cause environment seriously to pollute.
Mainly there are physical method, chemical method, bioanalysis etc. for the processing method of tricyclazole waste water at present.Wherein physical means
It the problem of such as absorption method, extraction generally existing secondary pollution, needs to be aided with other techniques, just can reach ideal removal
Rate；The middle-and-high-ranking oxidizing process of chemical method and electrochemical process energy consumption are high, and cost is also high, and the toxicity of partial intermediate is bigger.Therefore,
To high treating effect, the discussion of the processing technique of small, the without secondary pollution tricyclazole pollutant effluents of processing cost and technique, have
Theoretical and practical significance.
Microorganism treatment has the advantages that economical, efficient, it is often more important that Ke Yishi compared with physics, chemical method
The innoxious governance now polluted.Bioanalysis is without secondary pollution, treating capacity is big, is wastewater processing technology most widely used at present.But
Due to tricyclazole stable structure, bio-degradable degree is not high, and common biological treatment can not act on or inefficiency.Therefore,
Domestication, screening and the application of high to tricyclazole degradation efficiency, good degrading effect microorganism, are to solve tricyclazole contaminated wastewater
Effective way.However the biodegrade of heterocyclic compound this for tricyclazole is showed no document report both at home and abroad.
Summary of the invention
It is an object of the invention to be directed to the shortage of current tricyclazole degradation bacteria strains, one plant of tricyclazole degradation sphingol is provided
Bacterium Sphingomonas sp.NJUST37.
The present invention is to take dirt from Powdered Activated Carbon-activated sludge tank of bumper harvest Environmental Technology Services Co., Ltd, Jintan City
Then mud sample adds Mensurating Tricyclazole Technical and carries out acclimation to it, after domestication after a period of time, be inoculated with this sludge to three
Ring azoles has obtained tricyclazole degradation bacteria, has been through molecular biology identification to cultivate in the culture medium of sole carbon source, nitrogen source and sulphur source
Sphingomonas bacteria is named as Sphingomonas sp.NJUST37, and the GenBank number of logging in is KX523292, in September, 2016
In China typical culture collection center (CCTCC) preservation, deposit number was CCTCC NO:M 2016520, preservation address on 26th
For Wuhan University, Wuhan City.
It is a further object of the present invention to provide above-mentioned tricyclazole degradation sphingomonas bacteria Sphingomonas sp.NJUST37 to exist
Application in wastewater treatment containing tricyclazole.
Above-mentioned tricyclazole degradation sphingomonas bacteria Sphingomonas sp.NJUST37 answering in wastewater treatment containing tricyclazole
With, method particularly includes: tricyclazole degradation sphingomonas bacteria Sphingomonas sp.NJUST37 seed liquor is inoculated into containing tricyclazole
In waste water, cultivation temperature is 20~35 DEG C, and the pH of the waste water containing tricyclazole is 6.0-8.0.
Preferably, the cultivation temperature is 30 DEG C.
Preferably, the pH of the waste water containing tricyclazole be 6.5-7.5, further preferably 7.
Compared with prior art, the invention has the following advantages that
Tricyclazole degradation sphingomonas bacteria Sphingomonas sp.NJUST37 of the invention, can be unique with tricyclazole
Carbon source, nitrogen source and sulphur source are grown, and can be used for the bacterium of tricyclazole wastewater treatment for domestic outside strain.Sphingomonas bacteria
Sphingomonas sp.NJUST37 can realize that concentration is the processing of the tricyclazole waste water of 100mg/L, tricyclazole in 4d or so
Degradation rate reach 95% or more, and the degradable up to tricyclazole of 270mg/L.In the model that tricyclazole concentration is 0-180mg/L
In enclosing, Sphingomonas sp.NJUST37 normal growth and can realize the degradable of tricyclazole.
Detailed description of the invention
Fig. 1 be in waste water tricyclazole concentration to bacterial strain Sphingomonas sp.NJUST37 degradation tricyclazole performance shadow
Ring result figure.
Fig. 2 is the influence result figure of waste water temperature and pH to tricyclazole degradation property.
Fig. 3 is influence result figure of the presence of degradable carbon source in waste water to tricyclazole degradation property.
Fig. 4 is influence result figure of the presence inorganic nitrogen-sourced in waste water to tricyclazole degradation property.
Fig. 5 is that tricyclazole degradation front and back COD and TOC cuts down situation result figure.
Specific embodiment
Below with reference to embodiment and attached drawing, the invention will be further described.
Embodiment 1: the screening separation of tricyclazole degradation bacteria Sphingomonas sp.NJUST37 and its drop to tricyclazole
Solve performance
(1) separation of bacterial strain
Sludge sample is taken from Powdered Activated Carbon-activated sludge tank of bumper harvest Environmental Technology Services Co., Ltd, Jintan City, so
After add Mensurating Tricyclazole Technical acclimation carried out to it.After domestication in 1 month, take 5mL mud sample that 50mL tricyclazole is added dense
Degree is the liquid inorganic salt culture medium (MSM) of 50mg/L, is fitted into 100mL triangular flask, with 170 revs/min of turn under the conditions of 30 DEG C
Fast shaking table culture is taken out triangular flask after 4d and is stood, takes 2mL supernatant after 30min, and transferring into 50mL tricyclazole concentration is 50mg/L
Liquid inorganic salt culture medium (MSM) in, be fitted into 100mL triangular flask, trained under the conditions of 30 DEG C with 170 revs/min of revolving speed shaking table
It supports, realizes the enrichment culture of tricyclazole degradation bacteria.After 4 days, 2mL fluid nutrient medium is transferred into 50mL fresh culture, and shaking table
Culture.After continuous 3 times switchings, fluid nutrient medium is diluted 10 with sterile distilled water4-108Times, it is coated on tricyclazole concentration
For 50mg/L, using tricyclazole to be put into 30 DEG C of incubators in the inorganic salts solid medium tablets of sole carbon source, nitrogen source and sulphur source
It is cultivated.Picking has the bacterium colony of notable difference on colony characteristics after a week, is carried out using the method for plate streaking separation pure
Change, continuous purification three times after, obtain single bacterial strain, and carry out inclined-plane preservation.
The composition of minimal medium (MSM) is as follows: Na2HPO4·12H2O (3.057g/L), KH2PO4(0.760g/L),
MgCl2·4H2O (0.136g/L), CaCl2(0.05g/L), trace element solution SL-4 (10mL/L).Microelement SL-4 group
At: EDTA (0.5g/L), FeSO4·7H2O (0.2g/L), microelement SL-6 (100mL/L).Microelement SL-6 composition:
ZnSO4·7H2O (0.01g/L), MnCl2·4H2O (0.03g/L), H3BO4(0.3g/L), CoCl2·6H2O (0.2g/L),
CuCl2·2H2O (0.01g/L), NiCl2·6H2O (0.02g/L), Na2MoO4·2H2O(0.03g/L)。
(2) screening of bacterial strain
The single colonie of picking resulting separation, being inoculated in tricyclazole concentration respectively is 50mg/L, using tricyclazole as sole carbon
In the MSM of source and nitrogen source, shaking table culture 4d.Changed with tricyclazole concentration in high effective liquid chromatography for measuring culture medium, if training
Supporting tricyclazole concentration in base significantly reduces, that is, can be shown that tricyclazole is degraded.In isolated single colonie, it is named as
The strains for degrading performance of NJUST37 is the most excellent, and in the culture period of 2d, tricyclazole is degradable, therefore chooses bacterial strain work
Subsequent identification.
(3) identification of bacterial strain
Morphology, Physiology and biochemistry test are carried out to bacterium.The 16S rDNA sequence for measuring bacterial strain, by the 16S rDNA of bacterial strain
Sequence in gene order and world GenBank database carries out online tetraploid rice, and finally determining from molecular level should
The kind of bacterium.
1. morphological feature: NJUST37 bacterium colony is in yellow, round, and edge is serrated, and center is smooth；
2. physiological and biochemical property: Gram-negative, catalyzing enzyme is negative, oxidase positive, contacts enzyme positive.It is aerobic, have
Dynamic role, most suitable degradation pH range are 6.5-7.5, and optimum growth temperature is 30 DEG C.
3. molecular biology identification: using the core DNA of NJUST37 bacterium as template, with the logical of the PCR amplification of 16S rDNA gene
It is primer with primer, carries out PCR amplification, measure its complete sequence, the sequence of the 16S rDNA gene order of agrobacterium as shown in Figure 4
List.The 16S rDNA gene order of bacterial strain is committed to GenBank database (the GenBank number of logging in is KX523292), with
Sequence in GenBank database carries out online tetraploid rice, the results showed that, NJUST37 and Sphingomonas
The sequence similarity of paucimobilis isolate12H6 and Sphingomonas fuliginis strain TKP is up to
99% or more.
Sheath ammonia is accredited as according to the morphology of NJUST37, Physiology and biochemistry test and molecular biological analysis, NJUST37
Alcohol bacterium Sphingomonas sp., is named as Sphingomonas sp.NJUST37.
(4) degradation of the bacterial strain to tricyclazole and the application in tricyclazole wastewater treatment
Tricyclazole degradation bacteria strains NJUST37 is seeded to the MSM culture medium of addition 100mg/L tricyclazole, under the conditions of 30 DEG C
With 170 revs/min of revolving speed shaking table culture, the enrichment culture of NJUST37 is carried out, enters the logarithmic growth phase later period (about to thallus
3d), by gained thallus with 3, the revolving speed of 000 × g is centrifugated 5 minutes, skims supernatant, using the method for the concussion that is vortexed by bacterium
Body is resuspended in sterile liquid MSM, centrifugation.After repeated washing process four times, thallus is resuspended in sterile liquid MSM
(the MSM amount being added is adjusted, bacterium suspension optical density OD is controlled600About 0.1), seed liquor is obtained.
Preparing using 100mg/L tricyclazole is sole carbon source and the liquid MSM of nitrogen source as simulated wastewater, by above-mentioned seed liquor
It is added in simulation tricyclazole waste water, with 170 revs/min of revolving speed shaking table culture under the conditions of 30 DEG C, observes in degradation process in waste water
Tricyclazole concentration observes bacterial growth situation.Set up the blank control for not being inoculated with NJUST37.As shown in Figure 1,100mg/L tricyclic
Azoles can tricyclazole special efficacy degradation bacteria after being tamed in 4d it is degradable.And in the control sample for not being inoculated with NJUST37, tricyclic
Azoles does not obtain obvious degradation.
This example demonstrates that it is sole carbon source that isolated Sphingomonas sp.NJUST37, which can use tricyclazole,
Growth and breeding is carried out with nitrogen source, and can realize the degradable of tricyclazole.
Embodiment 2: influence of the tricyclazole concentration to tricyclazole degradation property
Preparing tricyclazole concentration is respectively the liquid MSM of 50mg/L, 100mg/L, 180mg/L and 270mg/L as simulation
Sphingomonas sp.NJUST37 seed liquor is accessed simulated wastewater with 4% inoculum concentration by waste water.As shown in Figure 1,
Sphingomonas sp.NJUST37 can realize complete drop of the tricyclazole at concentrations up to tricyclazole in the simulated wastewater of 180mg/L
Solution.Under conditions of tricyclazole concentration is 50mg/L, 100mg/L and 180, difference can realize simulation in 48h, 96h and 480h
Tricyclazole completely removes in waste water.High toxicity and characteristic difficult to degrade due to tricyclazole, at concentrations up under 270mg/L,
NJUST37 can realize 40% tricyclazole degradation after cultivating 500h.And under a high concentration condition, bacterial growth lag phase
It is obviously prolonged.
This example demonstrates that Sphingomonas sp.NJUST37 can realize the degradation of higher concentration tricyclazole, substantially may be used
To meet general use demand.
Embodiment 3: the influence of temperature and pH value to tricyclazole degradation property
Preparation tricyclazole concentration is 100mg/L, and the tricyclazole simulated wastewater that initial pH cultivation temperature is 7.0 will
Sphingomonas sp.NJUST37 seed liquor accesses simulated wastewater with 4% inoculum concentration, and being respectively placed in temperature is 20 DEG C, 25
DEG C, 30 DEG C, cultivate in 35 DEG C of shaking tables with 40 DEG C.As shown in Fig. 2 a, under conditions of cultivation temperature is 25 DEG C~35 DEG C,
Sphingomonas sp.NJUST37 can realize the high efficiency degradation of tricyclazole；Under conditions of cultivation temperature is 20 DEG C,
Sphingomonas sp.NJUST37 can also realize the degradation of tricyclazole, but degradation rate is significantly slower；And it is in cultivation temperature
Under conditions of 40 DEG C, tricyclazole cannot be degraded completely.
Preparation tricyclazole concentration is 100mg/L, and initial pH is the three of 4.0,5.0,6.0,6.5,7.0,7.5,8.0 and 9.0
Sphingomonas sp.NJUST37 seed liquor is accessed simulated wastewater with 4% inoculum concentration by ring azoles simulated wastewater.By Fig. 2 b
Shown, under conditions of initial pH is 6.5,7.0 and 7.5, Sphingomonas sp.NJUST37 can realize the efficient of tricyclazole
Rate degradation；Under conditions of initial pH is 6.0,8.0, Sphingomonas sp.NJUST37 can also realize the complete of tricyclazole
Degradation, but degradation rate is significantly slower；And and pH be 4.0,5.0 and 9.0 under conditions of, tricyclazole cannot almost be dropped
Solution.
This example demonstrates that the optimum culturing temperature of the degradation tricyclazole of Sphingomonas sp.NJUST37 is 30 DEG C,
Cultivation temperature is higher than 40 DEG C or can all inhibit the degradation of tricyclazole lower than 20 DEG C.In the range of pH is 6.0-8.0,
Sphingomonas sp.NJUST37 can realize the degradable of tricyclazole, and optimal pH condition is neutrality.To sum up, it is too low and
Excessively high temperature and pH condition can all inhibit the degradation of tricyclazole.
Embodiment 4: influence of the presence of degradable organic carbon source to tricyclazole degradation property
Preparation tricyclazole concentration is 100mg/L, additional degradable organic carbon source (sucrose, glucose and yeast extract)
Concentration is respectively the simulated wastewater of 500mg/L, 1000mg/L and 2000mg/L, by Sphingomonas sp.NJUST37 seed
Liquid accesses simulated wastewater with 4% inoculum concentration.As shown in Figure 3, the addition of the yeast extract of various concentration is for tricyclazole
Degradation rate is obviously improved；And the degradation for then inhibiting tricyclazole is added in different glucose and sucrose ferment；And Portugal
The inhibiting effect that grape sugar degrades to tricyclazole with sucrose ferment is increased with increasing for its concentration.
This example demonstrates that in addition to the extraction from yeast beyond the region of objective existence with nitrogen, other two kinds of degradable carbon sources there are unfavorable
In degradation of the Sphingomonas sp.NJUST37 to tricyclazole, the addition of degradable carbon source can degrade to tricyclazole obstruction
Effect, this may be to generate competition to the degradation of tricyclazole because the presence of degradable carbon source consumes oxygen and nutrient
Property inhibit.
Embodiment 5: influence of the inorganic nitrogen-sourced presence to tricyclazole degradation property
Preparation tricyclazole concentration is inorganic nitrogen-sourced (ammonium chloride, sodium nitrate and the nitrous acid of 100mg/L, additional 3 kinds of forms
Sodium) concentration be respectively 2mM, 5mM and 10mM simulated wastewater, Sphingomonas sp.NJUST37 seed liquor is connect with 4%
Kind amount access simulated wastewater.As shown in Figure 4, the addition of the sodium nitrite of the ammonium chloride of various concentration and sodium nitrate and 2mM for
The degradation rate of tricyclazole is obviously improved；And the addition of the sodium nitrite of 5mM then inhibits the degradation of tricyclazole in early period,
Stage plays the role of promoting degradation；The addition of the sodium nitrite of 10mM is then in the degradation for completely inhibiting tricyclazole.
This example demonstrates that removing the inorganic nitrogen-sourced addition of appropriate type and concentration, be conducive to Sphingomonas
Degradation of the sp.NJUST37 to tricyclazole, the addition of the nitrogen source of diversified forms, which can degrade to tricyclazole, is obviously promoted effect.This
May be because NJUST37 growth and breeding need nitrogen source amount be more than degrade tricyclazole caused by nitrogen source amount, cause
NJUST37 is in nitrogen hunger state.And the nitrite of high concentration has toxicity to NJUST37, its physiology can be inhibited living
It is dynamic, and then inhibit the degradation of tricyclazole.
The situation of change that embodiment 6:COD and TOC degrades with tricyclazole
Preparation tricyclazole concentration is 100mg/L, and the tricyclazole simulated wastewater that initial pH cultivation temperature is 7.0 will
Sphingomonas sp.NJUST37 seed liquor accesses simulated wastewater with 4% inoculum concentration, is placed in the shaking table that temperature is 30 DEG C
Culture.Proportional spacing is set, the COD and TOC of culture medium are measured.As shown in Figure 5, under the condition of culture of optimum temperature and pH,
Sphingomonas sp.NJUST37 can realize largely going for COD and TOC while the high efficiency degradation for realizing tricyclazole
It removes, final removal rate is all up to 60% or more.
This example demonstrates that Sphingomonas sp.NJUST37 to the degradation of tricyclazole more completely and thoroughly, reach
The ideal effect of biomineralization.And performance of the NJUST37 in terms of waste water COD removal has also provided for its industrial applications
Power is supported.
SEQUENCE LISTING
<110>Institutes Of Technology Of Nanjing
<120>one plants of tricyclazole degradation sphingomonas bacterias and its application in wastewater treatment containing tricyclazole
<130> 2016
<160> 1
<170> PatentIn version 3.5
<210> 1
<211> 1349
<212> DNA
<213>Sphingol single-cell
<400> 1
gcagtcgaac gagcctttcg gggctagtgg cgcacgggtg cgtaacgcgt gggaatctgc 60
ccttgggttc ggaataacgt ctggaaacgg acgctaatac cggatgatga cgaaagtcca 120
aagatttatc gcccagggat gagcccgcgt aggattagct agttggtgag gtaaaggctc 180
accaaggcga cgatccttag ctggtctgag aggatgatca gccacactgg gactgagaca 240
cggcccagac tcctacggga ggcagcagta gggaatattg gacaatgggg gcaaccctga 300
tccagcaatg ccgcgtgagt gatgaaggcc ttagggttgt aaagctcttt tacccgggat 360
gataatgaca gtaccgggag aataagcccc ggctaactcc gtgccagcag ccgcggtaat 420
acggaggggg ctagcgttgt tcggaattac tgggcgtaaa gcgcacgtag gcggcgattt 480
aagtcagagg tgaaagcccg gggctcaacc ccggaatagc ctttgagact ggattgcttg 540
aacatcggag aggtgagtgg aattccgagt gtagaggtga aattcgtaga tattcggaag 600
aacaccagtg gcgaaggcgg ctcactggac gattgttgac gctgaggtgc gaaagcgtgg 660
ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgata actagctgct 720
ggggctcatg gagtttcggt ggcgcagcta acgcattaag ttatccgcct ggggagtacg 780
gtcgcaagat taaaactcaa aggaattgac gggggcctgc acaagcggtg gagcatgtgg 840
tttaattcga agcaacgcgc agaaccttac caacgtttga catcctcatc gcgatttcca 900
gagatggatt tcttcagttc ggctggatga gtgacaggtg ctgcatggct gtcgtcagct 960
cgtgtcgtga gatgttgggt taagtcccgc aacgagcgca accctcgcct ttagttgcca 1020
tcatttagtt gggtactcta aaggaaccgc cggtgataag ccggaggaag gtggggatga 1080
cgtcaagtcc tcatggccct tacgcgttgg gctacacacg tgctacaatg gcgactacag 1140
tgggcagcga ccatgcgagt ggaagctaat ctccaaaagt cgtctcagtt cggattgttc 1200
tctgcaactc gagagcatga aggcggaatc gctagtaatc gcggatcagc atgccgcggt 1260
gaatacgttc ccaggccttg tacacaccgc ccgtcacacc atgggagttg gtttcacccg 1320
aaggcagtgc gctaaccgca aggaggcag 1349
Claims (6)
- The sphingomonas bacteria 1. one plant of tricyclazole is degraded, is sphingomonas bacteria Sphingomonas sp.NJUST37, deposit number is CCTCC NO:M 2016520.
- 2. application of the tricyclazole degradation sphingomonas bacteria according to claim 1 in wastewater treatment containing tricyclazole.
- 3. application of the tricyclazole degradation sphingomonas bacteria according to claim 2 in wastewater treatment containing tricyclazole, feature It is, method particularly includes: tricyclazole degradation sphingomonas bacteria Sphingomonas sp.NJUST37 seed liquor is inoculated into containing tricyclic In azoles waste water, cultivation temperature is 20~35 DEG C, and the pH of the waste water containing tricyclazole is 6.0-8.0.
- 4. application of the tricyclazole degradation sphingomonas bacteria according to claim 3 in wastewater treatment containing tricyclazole, feature It is, the cultivation temperature is 30 DEG C.
- 5. application of the tricyclazole degradation sphingomonas bacteria according to claim 3 in wastewater treatment containing tricyclazole, feature It is, the pH of the waste water containing tricyclazole is 6.5-7.5.
- 6. application of the tricyclazole degradation sphingomonas bacteria according to claim 3 in wastewater treatment containing tricyclazole, feature It is, the pH of the waste water containing tricyclazole is 7.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611204665.XA CN106591192B (en) | 2016-12-23 | 2016-12-23 | One plant of tricyclazole degradation sphingomonas bacteria and its application in wastewater treatment containing tricyclazole |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201611204665.XA CN106591192B (en) | 2016-12-23 | 2016-12-23 | One plant of tricyclazole degradation sphingomonas bacteria and its application in wastewater treatment containing tricyclazole |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN106591192A CN106591192A (en) | 2017-04-26 |
| CN106591192B true CN106591192B (en) | 2019-05-07 |
Family
ID=58603283
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201611204665.XA Active CN106591192B (en) | 2016-12-23 | 2016-12-23 | One plant of tricyclazole degradation sphingomonas bacteria and its application in wastewater treatment containing tricyclazole |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106591192B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1800352A (en) * | 2005-12-23 | 2006-07-12 | 南京农业大学 | Bacterium for degrading chlorpyrifos pesticide residue and produced bacterium formulation |
| CN1844361A (en) * | 2006-03-08 | 2006-10-11 | 华南理工大学 | Polycyclic aromatic hydrocarbon degrading bacteria and use thereof |
| JP2015211816A (en) * | 2014-05-03 | 2015-11-26 | ビオックス・テクノロジー株式会社 | Method for removing radioactive cesium and dioxins |
-
2016
- 2016-12-23 CN CN201611204665.XA patent/CN106591192B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1800352A (en) * | 2005-12-23 | 2006-07-12 | 南京农业大学 | Bacterium for degrading chlorpyrifos pesticide residue and produced bacterium formulation |
| CN1844361A (en) * | 2006-03-08 | 2006-10-11 | 华南理工大学 | Polycyclic aromatic hydrocarbon degrading bacteria and use thereof |
| JP2015211816A (en) * | 2014-05-03 | 2015-11-26 | ビオックス・テクノロジー株式会社 | Method for removing radioactive cesium and dioxins |
Also Published As
| Publication number | Publication date |
|---|---|
| CN106591192A (en) | 2017-04-26 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Perez‐Garcia et al. | Efficiency of growth and nutrient uptake from wastewater by heterotrophic, autotrophic, and mixotrophic cultivation of Chlorella vulgaris immobilized with Azospirillum brasilense 1 | |
| Yang et al. | Cultivation of oleaginous microalgae for removal of nutrients and heavy metals from biogas digestates | |
| CN104845920B (en) | One plant of ocean Zhuo Beier Salmonella and its application | |
| CN104673715B (en) | There is fixed effect to cadmium and enterobacteria and its application of plant growth can be promoted | |
| CN102443542B (en) | High-density culture process of autotrophic oil-producing microalgae | |
| CN105638527B (en) | A kind of culture of Penaeus vannamei method of green high yield | |
| CN104911130A (en) | Halomonas sp. with denitrogenation capability and application thereof | |
| CN100552019C (en) | One strain denitrogen paracoccus and cultural method thereof and application | |
| CN103805546A (en) | Acinetobacter johnsonii AJ-3 strain and application thereof | |
| Akizuki et al. | Nitrification of anaerobic digestate using a consortium of microalgae and nitrifiers in an open photobioreactor with moving bed carriers | |
| CN103204589A (en) | Method for biologically treating industrial circulating water and stabilizing quality thereof | |
| CN101139140A (en) | Aerugo microcapsule algae degradation method using animalcule | |
| CN103540544A (en) | Bacillus radicicola capable of degrading pyridine as well as breeding method and application thereof | |
| CN106635909A (en) | Crude oil degradation mixed bacterium, microbial agent and application of microbial agent | |
| CN104403965B (en) | A kind of roost rose of Sharon pseudomonad of water body tetracycline pollutant of degrading and its application | |
| CN103740615B (en) | Photosynthetic bacteria SC01 and fast culture process thereof and application | |
| CN104694435B (en) | One plant of Shinella sp. with triazole degradation function and its application | |
| CN103773714B (en) | The application of a kind of photosynthetic bacterium in sea farming water correction | |
| CN102531154B (en) | Method and device for culturing aerobic biofilm capable of treating low-concentration ammonia nitrogen in water body | |
| CN107841477A (en) | Application of one plant of arsenic oxidizing bacteria in rice trivalent arsenic pollution is reduced | |
| CN102337232B (en) | Aerobic denitrification psychrotolerant bacterium and preparation method thereof | |
| CN105385642B (en) | Shortwave monad and its application | |
| CN106591192B (en) | One plant of tricyclazole degradation sphingomonas bacteria and its application in wastewater treatment containing tricyclazole | |
| CN105217799A (en) | A kind of industrial fermentation method of molten algae streptomycete active substance | |
| CN106479942B (en) | A kind of bacillus thuringiensis and its application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20190604 Address after: Room 485, Building A, 606 Ningliu Road, Jiangbei New District, Nanjing City, Jiangsu Province Patentee after: Nanjing Runke Environment Co., Ltd. Address before: 210094 No. 200, Xiaolingwei, Jiangsu, Nanjing Patentee before: Nanjing University of Science and Technology |
|
| TR01 | Transfer of patent right |