CN103540544A - Bacillus radicicola capable of degrading pyridine as well as breeding method and application thereof - Google Patents
Bacillus radicicola capable of degrading pyridine as well as breeding method and application thereof Download PDFInfo
- Publication number
- CN103540544A CN103540544A CN201310374832.5A CN201310374832A CN103540544A CN 103540544 A CN103540544 A CN 103540544A CN 201310374832 A CN201310374832 A CN 201310374832A CN 103540544 A CN103540544 A CN 103540544A
- Authority
- CN
- China
- Prior art keywords
- pyridine
- degradable
- bacillus radicicola
- bacterial strain
- concentration
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Abstract
The invention discloses bacillus radicicola capable of degrading pyridine as well as a breeding method and application thereof. The bacillus radicicola is bacillus radicicola (Rhizobiumsp.) by identification, and named (Rhizobiumsp.) NJUST18; the log-in number of GenBank is JN106368. The bacterial strain is preserved in the China center for type culture collection (CCTCC) on March 28th, 2013; the preservation number is CCTCC NO:M 2013110. Enrichment of pyridine degradation bacteria is carried out by directly adopting a culture medium taking pyridine as sole carbon source and nitrogen source; separation is carried out by adopting a screening culture medium taking the pyridine as the sole carbon source and nitrogen source; the screening process is quick and fast; sundry bacteria on the culture medium are few; the workload of affination is reduced. Compared with the other pyridine degradation bacteria, the bacterial strain has efficient pyridine degradation capability, good adaptive capacity and tolerance, and has a good application prospect in treatment of a high-concentration pyridine wastewater.
Description
Technical field
The invention belongs to environmental organic pollutant biologic treating technique field, be specifically related to bacterium, selection and the application in biological wastewater treatment and environmental pollution reparation thereof of a strain degraded pyridine.
Background technology
Pyridine is widely used in the industries such as medicine, agricultural chemicals, chemical industry, is a kind of typical difficult degradation nitrogen-containing heterocycle compound.Water-soluble stronger because its heterocycle structure has of pyridine, is easy to transfer in soil and groundwater, thus in soil and groundwater ubiquity.Due to bio-toxicity, aberration inducing and the carcinogenic nature of pyridine, pyridine pollutes the mankind's health and ecotope has been caused to huge potential hazard.Therefore, contain the waste water of pyridine as directly discharged without processing, can cause severe contamination to environment.
At present, the research of pyridine waste water is mainly concentrated on the physical chemistry treatment technologies such as absorption, burning and oxidation both at home and abroad.Yet the materialization treatment technology costs such as absorption, burning and oxidation are generally higher, are only applicable to the processing of high-purity pyridine waste water, and the waste water pyridine content after processing is still greater than emission standard, is difficult to realize qualified discharge, still needs and further processes.Therefore, to treatment effect is good, processing cost is little, the treatment technology of pyridine pollutant effluents of non-secondary pollution and the discussion of technique, there is theoretical and practical significance.
Microorganism treatment is compared with physics, chemical process, has economy, efficient advantage, the more important thing is the innoxious governance that can realize pollution.Biological process non-secondary pollution, treatment capacity are large, are most widely used wastewater processing technologies at present.But because pyridines material bio-degradable degree is not high, common biological treatment cannot act on or inefficiency.Therefore, domestication, screening and the application of the microorganism of, good degrading effect high to pyridine degradable efficiency, be the effective way that solves pyridine contaminated wastewater.
Recent domestic researchist has carried out some screening operations about pyridine degradable bacteria, has occurred that some are about the report of biological degradation pyridine at present, yet the pyridine degradable bacteria kind of having seen is at present still very limited, mainly comprise Arthrobacte, Bacillus, Lysinibacillus, Nocardiodes, Paracoccus, Pseudomonas, Rhodococcus, Shinella, Shewanella, the kinds such as Streptomyces.Yet, there is not so far the report about bacillus radicicola (Rhizobium sp.) degraded pyridine.In addition, most of pyridine degradable bacteria of having reported is at present to the tolerance concentration of pyridine low (being less than 1000mg/L), and degradation rate is slow, is difficult to meet the requirement of practical engineering application.Degradation efficiency is high, high to pyridine tolerance concentration, can adapt to true environment screening tool novel, efficient degrading bacteria be of great significance.
Summary of the invention
The object of the invention is to, for deficiencies such as current pyridine degradable bacterial strain are low to pyridine tolerance concentration, processing efficiency is low, bacterial strain kind is comparatively single, provides novel strain (Rhizobium sp.) NJUST18 of a highly effective degrading pyridine.
Another object of the present invention is to provide the selection of novel strain (Rhizobium sp.) NJUST18 of a highly effective degrading pyridine.
A further object of the invention is to provide (Rhizobium sp.) NJUST18 in the application containing pyridine field of waste water treatment.
Realizing the object of the invention technical solution is: the present invention is subject to for a long time pyridine contaminated soil separated, screening from Liuhe District, Nanjing chemical enterprise and has obtained pyridine special efficacy degradation bacteria strains NJUST18, through being accredited as bacillus radicicola (Rhizobium sp.), called after (Rhizobium sp.) NJUST18, the GenBank number of logging in is JN106368, bacterial strain on March 28th, 2013 in Chinese Typical Representative culture collection center (CCTCC) preservation, deposit number is CCTCC NO:M 2013110.This bacterial strain is the bacillus radicicola that domestic and international the first strain can be used for pyridine wastewater treatment.
The selection of the novel strain of one highly effective degrading pyridine (Rhizobium sp.) NJUST18, comprises the following steps:
(1) separation of bacterial strain: the soil sample 2g that the sewage draining exit that is subject to for a long time pyridine pollution is taken out joins 50mL containing in the liquid minimal medium MSM of 500mg/L pyridine, pack in 150mL triangular flask, rotating speed shaking table with 180 revs/min under 30 ℃ of conditions is cultivated, and realizes the enrichment culture of pyridine degradable bacteria; After 7 days, the liquid nutrient medium after 2mL enrichment culture is transferred in 50mL fresh liquid minimal medium MSM, and shaking table is cultivated; After continuous 3 switchings, with sterile distilled water, the liquid nutrient medium after switching is diluted to 10
5-10
9doubly, coat on the inorganic salt solid medium flat board containing 1000mg/L pyridine, put into 30 ℃ of incubators and cultivate; Picking has the bacterium colony of notable difference on colony characteristics after one week, adopts the method for plate streaking separation to carry out purifying, after continuous purification three times, obtains single bacterial strain, and carries out inclined-plane preservation.
(2) screening of bacterial strain: single bacterium colony of picking resulting separation, be inoculated in respectively in the inorganic salt liquid substratum containing 1000mg/L pyridine, shaking table is cultivated 120h; Measure pyridine change in concentration in substratum, choose the significantly reduced single bacterium colony of pyridine concentration in substratum.
Compared with prior art, the present invention has the following advantages:
(Rhizobium sp.) provided by the present invention NJUST18, the pyridine of can take is grown as sole carbon source, nitrogenous source.The present invention directly adopts take pyridine and carries out the enrichment of pyridine degradable bacteria as the substratum of sole carbon source and nitrogenous source, and adopt and take the screening culture medium that pyridine is sole carbon source, nitrogenous source and carry out separation, screening process is quick rapidly, and on this substratum, miscellaneous bacteria is less, has reduced the workload of multiple sieve.
The applied research of pyridine waste water shows, (Rhizobium sp.) NJUST18 that screening obtains, and can in 102h, realize concentration is the processing of the pyridine waste water of 1000mg/L, the degradation rate of pyridine and TOC reaches respectively 100% and 85%.In the scope that is 0-2600mg/L in pyridine concentration, (Rhizobium sp.) NJUST18 can normal growth and is realized the degradable of pyridine.In the scope that is 5.0-10.0 at pH, (Rhizobium sp.) NJUST18 can realize the degradable of pyridine.Lower concentration is easily degraded and can be played a driving role to pyridine degradable adding of carbon source.Compare with other pyridine degradable bacterial strains, this bacterial strain has efficient pyridine degradable ability, good adaptive faculty and tolerance performance, in the processing of high-purity pyridine waste water, has a good application prospect.
Accompanying drawing explanation
Fig. 1 is flat-plate bacterial colony figure (a) and the transmission electron microscope picture (b) of bacterial strain of the present invention.
Fig. 2 is the growth curve of bacterial strain of the present invention (Rhizobium sp.) NJUST18, to the release of ammonia nitrogen in the degradation curve of pyridine, degradation process and the changing conditions of TOC.
Fig. 3 be in waste water of the present invention pyridine concentration on bacterial strain (Rhizobium sp.) growth of NJUST18 and the impact of pyridine degradable.
Fig. 4 is the impact of wastewater pH of the present invention on pyridine degradable.
Fig. 5 is the easily impact of the existence of degraded carbon source on pyridine degradable in waste water of the present invention.
Fig. 6 is the 16SrDNA sequence table of bacterial strain of the present invention (Rhizobium sp.) NJUST18.
Embodiment
The following examples can make the present invention of those skilled in the art comprehend, but do not limit the present invention in any way.
Embodiment 1: screening and separating and the degradation property to pyridine thereof of pyridine degradable bacteria (Rhizobium sp.) NJUST18
(1) the separation of bacterial strain
The sewage draining exit that is subject to for a long time pyridine pollution from Liuhe District, Nanjing chemical enterprise takes soil sample, 2g soil sample is joined in the liquid minimal medium (MSM) that 50mL pyridine concentration is 500mg/L, pack in 150mL triangular flask, rotating speed shaking table with 180 revs/min under 30 ℃ of conditions is cultivated, and realizes the enrichment culture of pyridine degradable bacteria.After 7 days, the substratum after 2mL enrichment culture turns and is linked in 50mL fresh culture, and shaking table is cultivated.After continuous 3 switchings, with sterile distilled water, the liquid nutrient medium after switching is diluted to 10
5-10
9doubly, on the inorganic salt solid medium flat board that to coat pyridine concentration be 1000mg/L, the pyridine of take is sole carbon source and nitrogenous source, put into 30 ℃ of incubators and cultivate.Picking has the bacterium colony of notable difference on colony characteristics after one week, adopts the method for plate streaking separation to carry out purifying, after continuous purification three times, obtains single bacterial strain, and carries out inclined-plane preservation.
Minimal medium MSM's is composed as follows: Na
2hPO
412H
2o (1.529g/L), KH
2pO
4(0.372g/L), MgSO
47H
2o (0.1g/L), CaCl
2(0.05g/L), trace element solution SL-4 (10mL/L).Trace element SL-4 forms: EDTA (0.5g/L), FeSO
47H
2o (0.2g/L), micro-SL-6 (100mL/L).Trace element SL-6 forms: ZnSO
47H
2o (0.01g/L), MnCl
24H
2o (0.03g/L), H
3bO
4(0.3g/L), CoCl
26H
2o (0.2g/L), CuCl
22H
2o (0.01g/L), NiCl
26H
2o (0.02g/L), Na
2moO
42H
2o (0.03g/L).
(2) the screening of bacterial strain
Single bacterium colony of picking resulting separation, in the inorganic salt liquid substratum that to be inoculated in respectively pyridine concentration be 1000mg/L, the pyridine of take is sole carbon source and nitrogenous source, shaking table is cultivated 120h.Measure pyridine change in concentration in substratum, if pyridine concentration significantly reduces in substratum, can show that degraded has occurred pyridine.In the single bacterium colony obtaining in separation, the strains for degrading performance of called after NJUST18 is the most excellent, and in the incubation period of 120h, pyridine is degradable, and therefore choosing this bacterial strain does follow-up evaluation.
(3) the evaluation of bacterial strain
Bacterium is carried out to morphology, Physiology and biochemistry test.Measure the 16S rDNA sequence of bacterial strain, the sequence in the 16S rDNA gene order of bacterial strain and international GenBank database is carried out to online homology comparison, finally from molecular level, determine the kind of this bacterium.
1. morphological specificity: NJUST18 bacterium colony is white in color, circle, neat in edge, smooth moistening.This strain cell is rod-short, and smooth surface, with peritrichous motion, does not form gemma.Be of a size of 2-3 μ m * 0.8-0.9 μ m.In Fig. 1, a left side is the bacterium colony on plate culture medium, and the right side is the transmission electron microscope photo of bacterium.
2. physiological and biochemical property: Gram-negative, katalaze enzyme is negative, oxidase negative, and catalase is negative, urease-negative, can not hydrolyzed starch, can not hydrolyzed casein, can not utilize citric acid, can in the nutrient broth that be 9.0 at pH, grow, aerobic, the suitableeest degraded pH scope is 5.0-8.0, and optimum growth temperature is 20-35 ℃.
3. molecular biology identification: the core DNA of NJUST18 bacterium of take is template, the universal primer of pcr amplification of 16S rDNA gene of take is primer, carries out pcr amplification, measures its complete sequence, the sequence table as shown in Figure 6 of the 16S rDNA gene order of edaphic bacillus.The 16S rDNA gene order of bacterial strain is committed to GenBank database (the GenBank number of logging in is JN106368), carry out online homology comparison with the sequence in GenBank database, result shows, the sequence similarity degree of NJUST18 and Rhizobium sp.R-24658 and Rhizobiales bacterium D11-28.1 is up to more than 99%.
According to the morphology of NJUST18, Physiology and biochemistry test and molecular biological analysis, NJUST18 is accredited as bacillus radicicola (Rhizobium sp.), called after (Rhizobium sp.) NJUST18.
(4) bacterial strain is to the degraded of pyridine and the application in pyridine wastewater treatment
Pyridine degradable bacterial strain NJUST18 is seeded to the LB substratum that adds 1000mg/L pyridine, rotating speed shaking table with 180 revs/min under 30 ℃ of conditions is cultivated, carry out the enrichment culture of NJUST18, treat that thalline enters the logarithmic phase later stage (about 48h), by the rotating speed centrifugation of 6000 * g 5 minutes for gained thalline, skim supernatant liquor, the method that adopts vortex concussion by thalline Eddy diffusion in sterile liquid MSM, centrifugal.After repeated washing process three times, thalline Eddy diffusion is regulated to the MSM amount adding in sterile liquid MSM(, control bacterium suspension OD
600be about 1.5), obtain seed liquor.
Preparation adds the liquid MSM of 1000mg/L pyridine as simulated wastewater, above-mentioned seed liquor is added in simulation pyridine waste water, rotating speed shaking table with 180 revs/min under 30 ℃ of conditions is cultivated, in observation degradation process, in waste water, pyridine concentration, total organic carbon (TOC) concentration, ammonia nitrogen concentration and pH change, observation bacterial growth situation.Set up the blank of not inoculating NJUST18.Experimental result as shown in Figure 2.As shown in Figure 2,1000mg/L pyridine can be degradable in 102h; Be accompanied by the degraded of pyridine, bacterial biomass phenomenal growth, yield coefficient is about 0.46; In pyridine degradable process, the nitrogen in pyridine structure discharges with the form of ammonia nitrogen, and the yield that pyridine degradable finishes rear ammonia nitrogen is about 55%; TOC significantly reduces, and clearance can be up to 85%; Due to the release of ammonia nitrogen, pH is increased to 8.76 by initial value 7.0.And in not inoculating the control sample of NJUST18, pyridine does not obtain obvious degradation.
Separated (Rhizobium sp.) NJUST18 obtaining of the present embodiment explanation can utilize pyridine to carry out growth and breeding for sole carbon source and nitrogenous source, and can realize the mineralising of pyridine.
Embodiment 2: the impact of pyridine concentration on pyridine degradable performance in waste water
The liquid MSM that preparation pyridine concentration is respectively 600mg/L, 1000mg/L, 1600mg/L, 2000mg/L and 2600mg/L is as simulated wastewater, will (Rhizobium sp.) NJUST18 seed liquor with 5% inoculum size, accesses simulated wastewater.As shown in Figure 3, (Rhizobium sp.) NJUST18 can realize degradable up to pyridine in the simulated wastewater of 2600mg/L of pyridine concentration.In pyridine concentration, be under the condition of 600mg/L, 1000mg/L, 1600mg/L, 2000mg/L and 2600mg/L, can in 75h, 102h, 204h, 220h, 240h, realize simulated wastewater respectively in the removal completely of pyridine.Follow the degraded of pyridine, bacterial concentration simultaneous growth, yield coefficient is respectively 0.51,0.46,0.33,0.35 and 0.34.Due to high toxicity and the difficult degradation characteristic of pyridine, under High Concentration Situation, although can realize the degradable of pyridine, its degradation rate significantly declines.The phenomenon that degradation rate declines is particularly evident latter stage in degraded.
Although the present embodiment explanation (Rhizobium sp.) NJUST18 can realize the degradable of high-purity pyridine, processing efficiency haves much room for improvement.
Embodiment 3: the impact of waste water ph on pyridine degradable performance
Preparation pyridine concentration is 1000mg/L, and initial pH is 5.0,6.0,7.0,8.0,9.0,10.0 pyridine simulated wastewater, will (Rhizobium sp.) NJUST18 seed liquor with 5% inoculum size, accesses simulated wastewater.As shown in Figure 4, under the condition that is 7.0-8.0 at initial pH, (Rhizobium sp.) NJUST18 can realize the high-level efficiency degraded of pyridine; Under the condition that is 5.0-6.0 at initial pH, experienced after the lag phase of 72h pyridine accelerated degradation; And under the condition that is 9.0-10.0 at pH, pyridine degradable is comparatively slow, particularly slow latter stage in degraded.
The optimal ph condition of the degraded pyridine of the present embodiment explanation (Rhizobium sp.) NJUST18 is neutral to weakly alkaline; In the scope that is 5.0-8.0 at pH, pyridine degradable speed is relatively high; High pH condition is unfavorable for the degraded of pyridine.
Embodiment 4: the easily impact of the existence of degraded carbon source on pyridine degradable performance in waste water
Preparation pyridine concentration is 1000mg/L, and additional glucose concn is respectively the pyridine simulated wastewater of 500mg/L, 1000mg/L, 2000mg/L, will (Rhizobium sp.) NJUST18 seed liquor with 5% inoculum size, accesses simulated wastewater.As shown in Figure 5,500mg/L glucose adds the degradation rate that contributes to promote pyridine; 1000mg/L, 2000mg/L glucose add the degraded that has delayed pyridine, and along with the increasing of additional glucose concn, retarding action aggravation.
The easy degraded carbon source of the present embodiment explanation lower concentration have a growth that is beneficial to (Rhizobium sp.) NJUST18, thereby promoted the degradation process of pyridine; The existence of the easy degraded carbon source of high density has consumed oxygen and nutritive element, and the competing property of degraded of pyridine is suppressed.
Claims (5)
1. the bacillus radicicola of a strain degradable pyridine, it is characterized in that it on March 28th, 2013 in Chinese Typical Representative culture collection center C CTCC preservation, depositary institution address is Hubei China province Wuhan City Wuhan University, deposit number is CCTCC NO:M2013110, called after bacillus radicicola NJUST18, its Classification And Nomenclature is (Rhizobium sp.), and the GenBank number of logging in is JN106368.
2. the bacillus radicicola of degradable pyridine according to claim 1, it is characterized in that described bacillus radicicola colony characteristics and physiological and biochemical property are: be white in color, circular, neat in edge, smooth moistening, this strain cell is rod-short, smooth surface, with peritrichous motion, do not form gemma, be of a size of 2-3 μ m * 0.8-0.9 μ m; Gram-negative, katalaze enzyme is negative, oxidase negative, catalase is negative, urease-negative.
3. a selection for the bacillus radicicola of degradable pyridine as claimed in claim 1, is characterized in that comprising the following steps:
(1) separation of bacterial strain: the soil sample 2g that the sewage draining exit that is subject to for a long time pyridine pollution is taken out joins 50mL containing in the liquid minimal medium MSM of 500mg/L pyridine, pack in 150mL triangular flask, under 30 ℃ of conditions, with the rotating speed shaking table of 180 revs/min, cultivate and carry out enrichment culture; After 7 days, the liquid nutrient medium after 2mL enrichment culture is transferred in 50mL fresh liquid minimal medium MSM, and shaking table is cultivated; After continuous 3 switchings, with sterile distilled water, the liquid nutrient medium after switching is diluted to 10
5-10
9doubly, coat on the inorganic salt solid medium flat board containing 1000mg/L pyridine, put into 30 ℃ of incubators and cultivate; Picking has the bacterium colony of notable difference on colony characteristics after one week, adopts the method for plate streaking separation to carry out purifying, after continuous purification three times, obtains single bacterial strain, and carries out inclined-plane preservation.
(2) screening of bacterial strain: single bacterium colony of picking resulting separation, be inoculated in respectively in the inorganic salt liquid substratum containing 1000mg/L pyridine, shaking table is cultivated 120h; Measure pyridine change in concentration in substratum, choose the significantly reduced single bacterium colony of pyridine concentration in substratum.
4. a bacillus radicicola that utilizes the degradable pyridine described in claim 1 application in pyridine waste water treatment.
5. the application of the bacillus radicicola of degradable pyridine according to claim 4 in pyridine waste water treatment, is characterized in that the pH value of described pyridine waste water is 5-8, and in waste water, pyridine concentration is no more than 2600mg/L.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310374832.5A CN103540544B (en) | 2013-08-23 | 2013-08-23 | Bacillus radicicola capable of degrading pyridine as well as breeding method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310374832.5A CN103540544B (en) | 2013-08-23 | 2013-08-23 | Bacillus radicicola capable of degrading pyridine as well as breeding method and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103540544A true CN103540544A (en) | 2014-01-29 |
| CN103540544B CN103540544B (en) | 2015-04-29 |
Family
ID=49964406
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310374832.5A Active CN103540544B (en) | 2013-08-23 | 2013-08-23 | Bacillus radicicola capable of degrading pyridine as well as breeding method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103540544B (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105713862A (en) * | 2016-03-28 | 2016-06-29 | 武汉科技大学 | Bacterial strain capable of degrading pyridine and ammonia nitrogen, preparation method and application of bacterial strain |
| CN105802894A (en) * | 2016-05-13 | 2016-07-27 | 南京理工大学 | Pyridine degrading bacteria and application thereof in pyridine-containing wastewater treatment |
| CN106047746A (en) * | 2016-05-13 | 2016-10-26 | 南京理工大学 | Pyridine degrading pigmentiphaga sp. and applications thereof in treatment for pyridine-containing wastewater |
| CN106399194A (en) * | 2016-10-27 | 2017-02-15 | 安徽师范大学 | Pyridine degradation strain A6, fungicide produced by same and application thereof |
| WO2022087870A1 (en) * | 2020-10-26 | 2022-05-05 | 南京江岛环境科技研究院有限公司 | Rhizobium and use thereof in degradation of nonylphenol in water environment |
| CN116396898A (en) * | 2023-03-10 | 2023-07-07 | 江苏诚冉环境修复工程有限公司 | 1, 2-trichloroethane degrading bacterium and application thereof |
| CN116837068A (en) * | 2023-07-12 | 2023-10-03 | 上海信诺佰世医学检验有限公司 | Separation and identification method under multi-factor influence of bacterial strain |
-
2013
- 2013-08-23 CN CN201310374832.5A patent/CN103540544B/en active Active
Non-Patent Citations (3)
| Title |
|---|
| MUDLIAR S N,ET AL: "Pyridine biodegradation in a novel rotating rope bioreactor", 《BIORESOURCE TECHNOLOGY》 * |
| 乔琳等: "吡啶降解菌的生物降解特性", 《清华大学学报（自然科学版）》 * |
| 李叶珺等: "吡啶降解菌的筛选及作用机理研究", 《太原理工大学学报》 * |
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105713862A (en) * | 2016-03-28 | 2016-06-29 | 武汉科技大学 | Bacterial strain capable of degrading pyridine and ammonia nitrogen, preparation method and application of bacterial strain |
| CN105713862B (en) * | 2016-03-28 | 2019-04-02 | 武汉科技大学 | The bacterial strain and its application of degradable pyridine and ammonia nitrogen |
| CN105802894A (en) * | 2016-05-13 | 2016-07-27 | 南京理工大学 | Pyridine degrading bacteria and application thereof in pyridine-containing wastewater treatment |
| CN106047746A (en) * | 2016-05-13 | 2016-10-26 | 南京理工大学 | Pyridine degrading pigmentiphaga sp. and applications thereof in treatment for pyridine-containing wastewater |
| CN105802894B (en) * | 2016-05-13 | 2019-05-07 | 南京理工大学 | One pyridine degradation bacterium strain and its application in wastewater treatment containing pyridine |
| CN106047746B (en) * | 2016-05-13 | 2019-06-25 | 南京理工大学 | One plant of pyridine degradable bites dyestuff bacterium and its application in wastewater treatment containing pyridine |
| CN106399194A (en) * | 2016-10-27 | 2017-02-15 | 安徽师范大学 | Pyridine degradation strain A6, fungicide produced by same and application thereof |
| CN106399194B (en) * | 2016-10-27 | 2019-07-02 | 安徽师范大学 | Microbial inoculum and the application of one pyridine degradation bacterium strain strain A6 and its production |
| WO2022087870A1 (en) * | 2020-10-26 | 2022-05-05 | 南京江岛环境科技研究院有限公司 | Rhizobium and use thereof in degradation of nonylphenol in water environment |
| CN116396898A (en) * | 2023-03-10 | 2023-07-07 | 江苏诚冉环境修复工程有限公司 | 1, 2-trichloroethane degrading bacterium and application thereof |
| CN116837068A (en) * | 2023-07-12 | 2023-10-03 | 上海信诺佰世医学检验有限公司 | Separation and identification method under multi-factor influence of bacterial strain |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103540544B (en) | 2015-04-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103540544B (en) | Bacillus radicicola capable of degrading pyridine as well as breeding method and application thereof | |
| CN104911130B (en) | One plant of Halomonas with denitrification ability and its application | |
| CN104845920B (en) | One plant of ocean Zhuo Beier Salmonella and its application | |
| CN102747014B (en) | High-saline biological denitrification salinivibrio strain and application thereof in wastewater treatment | |
| CN104862260B (en) | One plant of arthrobacterium and its application with aerobic denitrification ability | |
| CN105713862B (en) | The bacterial strain and its application of degradable pyridine and ammonia nitrogen | |
| CN105420165A (en) | Aerobic denitrifying bacteria and applications therefor | |
| CN102703349B (en) | Small brevibacterium strain capable of carrying out biological denitrification under high-salt condition and application of small brevibacterium strain in wastewater treatment | |
| CN101386823A (en) | Special effect anaerobic denitrifying bacterium and waste water processing method using thereof | |
| CN108611291A (en) | One plant of salt tolerance planococcus and its application | |
| CN103602613B (en) | Phenol-resistant heterotrophic nitrification-aerobic denitrification strain and applications thereof | |
| CN109337832A (en) | A kind of anthropi of resistance to high ammonia nitrogen heterotrophic nitrification-aerobic denitrification and its application | |
| CN101386822B (en) | Special effect phosphate accumulating organisms and waste water processing method using thereof | |
| CN104560728B (en) | One plant of trichoderma viride with ethyl acetate degradation capability and its application | |
| CN101811779A (en) | Preparation method of halophilic decontamination bacterial agent and bacterial agent prepared by same | |
| CN104694435A (en) | Shinella with triazole degradation function and application thereof | |
| CN103602619B (en) | The Soxhlet bacterium of one strain degradable triethylamine, selection and application | |
| CN101811780B (en) | Preparation method and application of halophilic decontamination bacterial agent | |
| CN102220265B (en) | Denitrifying bacterial strain and application of the same in water body for nitrogen removal together with wetland plants | |
| CN104004674B (en) | Aerobic denitrifying bacterial strain | |
| CN106591181A (en) | Arthrobactermysorens and application thereof to purification of marine-culture nitrogen-containing wastewater | |
| CN105802894A (en) | Pyridine degrading bacteria and application thereof in pyridine-containing wastewater treatment | |
| CN102277314B (en) | Screening method of efficient heterotrophic nitrifying bacteria as well as screen bacterial strains and preparation method of microbial inoculum thereof | |
| CN101054241A (en) | Application of flavobacterium omnivorum in treating sewage at low temperature | |
| CN101993833B (en) | Heterotrophy denitrifying bacteria for synchronously degrading sulfide, nitrate and organic carbon source |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| EE01 | Entry into force of recordation of patent licensing contract | ||
| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20140129 Assignee: JIANGSU YIYU ENVIRONMENTAL PROTECTION TECHNOLOGY CO.,LTD. Assignor: NANJING University OF SCIENCE AND TECHNOLOGY Contract record no.: X2023980030534 Denomination of invention: A Pyridine Degradable Rhizobium, Its Breeding Method and Application Granted publication date: 20150429 License type: Exclusive License Record date: 20230111 |