CN1065882A

CN1065882A - The prescription and the production technique of prawn vibriosis ecological regulation and control vibrio counting agent

Info

Publication number: CN1065882A
Application number: CN92107256A
Authority: CN
Inventors: 贺天笙; 杨立艾
Original assignee: JIANGSU SALT AND AQUATIC PRODUCTS CO
Current assignee: JIANGSU SALT AND AQUATIC PRODUCTS CO
Priority date: 1991-12-30
Filing date: 1991-12-30
Publication date: 1992-11-04

Abstract

A kind of prescription and production technique of prawn vibriosis ecological regulation and control vibrio counting agent are pressed the certain weight ratio preparation with five kinds of materials, and nutrition partly accounts for 70-99%, mainly contain poly-peptone, tryptone; Biostimulation partly accounts for 0.5-10%, mainly contains genseng; Reaction substrate accounts for 0.1-5%, mainly contains inositol and N.F,USP MANNITOL; Inhibitor partly accounts for 0.5-10%, mainly contains kantlex and Prostaphlin; Indicating section accounts for 0.5-5%, mainly contains acid fuchsin and bromothymol blue, and these materials separately by after certain explained hereafter, are cooperated by a certain percentage and produce counting agent of the present invention.

Description

The prescription and the production technique of prawn vibriosis ecological regulation and control vibrio counting agent

The present invention relates to calculate the demonstration medicament of lonely bacterium quantity in the prawn pool.

Do not detect the reagent of lonely bacterium quantity in the prawn pool in the prior art, the viewpoint of the relevant eubiosis sees that work is at the No1 in 1991 of ocean science editorial office, P65-66 page or leaf: the once breakthrough on the prevention and cure of shrimp disease history-" light in sea ", the brief introduction of physiological ecological therapy.But prawn do not narrated in this literary composition and lonely bacterium, prawn suffer from the bacterium amount condition and the easy lonely bacterium counting means of vibriosis, can't check out the lonely bacterium quantity in the prawn pool in practice very easily, causes difficulty for control prawn vibriosis.

Task of the present invention is at the deficiencies in the prior art, and the prescription and the production technique of the counting agent of lonely bacterium quantity in the prawn pool can be conveniently checked out in development.

The present invention finishes the work like this: lonely bacterium needs materials such as N.F,USP MANNITOL, glucose, inositol, citrate three sodium in growth, in lonely bacteria growing metabolism, produce metabolite, these metabolites can make indication mechanism produce the obvious color variation, and Chi Shuizhong contains 10-10 ²The lonely bacterium number of/Ml, Aeromonas and other bacterium are normal fishes and shrimps water, add behind the water sample in 10 minutes by light rose-red-blueness, represent no lonely bacteria growing, change yellow-flesh pink by green, and liquid muddiness in the bottle, expression has lonely bacteria growing, is converted into standard temperature according to color, can find the quantity of lonely bacterium the pond or Aeromonas from the double logarithmic curve table that designs, for bionomic control prawn vibriosis provides lonely bacterium data.Lonely bacterium counting indicator is made up of nutrition part, biostimulation agent part, reaction substrate, inhibition part, indicating section.Their proportioning is as follows respectively:

1, nutrition partly is to be mixed by poly-peptone, tryptone, lactoalbumin hydrolysate, yeast extract powder, or by poly-peptone account for weight ratio 60-70%, tryptone accounts for weight ratio 30-40% and mixes separately, or in all wts, accounting for 40-60% by poly-peptone, all the other respectively account for all weight percents and are respectively: tryptone accounts for that 20-30%, lactoalbumin hydrolysate account for 10-20%, yeast extract accounts for 5-10% and mixes.

2, the biostimulation agent partly is that genseng, northern stilbene, Radix Codonopsis are formed, also can pulverize 200 sieves with genseng separately, also can be respectively genseng 40-70%, northern stilbene 20-30% by above-mentioned three kinds of substances in percentage by weight, Radix Codonopsis is decided 10-30%, pulverized 200 mesh sieves after weighing jointly, the 2-4 times of organic solvent extraction that adds above-mentioned weight, after four extractions, add 0.05-1% medical active decolouring 2 times, be lower than the following temperature decompression of four degrees celsius, drying gets pale yellow mixture, adds the sodium sulphite anhydrous 99.3 mixing and mixes all.

3, reaction substrate is by N.F,USP MANNITOL 20-40%, glucose 20-40%, inositol 10-30%, trisodium citrate 5-10%; Or mix by N.F,USP MANNITOL 60-70%, inositol 30-40% and to form.

4, suppressing part is to pulverize 400 mesh sieves and Totazina, Prostaphlin, penicillin, kantlex by weight ratio by the Viola crystallina of 1.5-10% respectively to account for 2.5-10%, ametycin 0.5-10%, inositol 50-70% or mix generation by Prostaphlin 50-60%, kantlex 40-50%

5, indicating section is to be respectively toluylene red 5-10%, rhodizonic acid 1.5-5 acid fuchsin 30-50% pulverizing by weight ratio to mix 400 mesh sieves, thymol blue 5-20%, bromothymol blue 5-20%, the common grind up of anhydrous sodium carbonate 5-10% is not crossed 400 mesh sieves and is generated; Or be crushed into powder by acid fuchsin 40-60%, bromothymol blue 30-50%.

Above-mentioned five kinds of materials are prepared in following ratio: (weight percent)

Nutrition part 70-99%

Biostimulation part 0.5-10%

Reaction substrate 0.1-5%

Inhibitor part 0.5-10%

Indicating section 0.5-5%

With above-mentioned each 100% proportioning of partly always weighing, at 4 ℃-20 ℃, relative humidity is lower than in 60% the sterilisable chamber in working spaces's temperature, and physical condition mixes can produce indicator of the present invention.Adjust aforementioned proportion can produce various different purposes the counting agent of the lonely bacterium quantity of prawn vibriosis.

Most preferred embodiment of the present invention is as follows: the sea-water type indicator is partly pressed 70-80% with each part weight ratio by nutrition, reaction substrate is pressed 5-10%, biostimulation agent 3-10%, suppress part and press 3-10%, indicating section 2-5% weighs, be lower than under 60% aseptic condition in 4 ℃-25 ℃ of room temperatures, relative humidity, indicating section and inhibition part are mixed, biostimulation agent and reaction substrate are mixed, after being mixed, two parts partly are mixed with nutrition again, continuation is mixed in moisture eliminator repeatedly, and sterile state is sub-packed in sealing preservation in the vial.

Embodiment 2: fresh water type indicator, weight ratio nutrition press partly that 70-80%, the low thing of reaction partly press 8-12%, the biostimulation agent partly press 3-6%, inhibitor and partly press 4-8%, indicates part to press 2-4%, and specifically processing method is with embodiment 1.

When the said counting agent is used, temperature of reaction is an optimum temps with 35 ℃, in counting agent, inject water sample, shake evenly wait to become blueness after, put into the 35C thermostat container, timing after 10 minutes, the used time is the time of tabling look-up, other temperature of reaction need be converted into 35 ℃ reaction times, during pond water potential of hydrogen PH7.0-9.0 to be measured reaction is not all had obviously influence, and the critical bacterium that vibriosis takes place is worth 10 ⁵/ ml water, by the colour-change time look into ordinate be the time (hour), abscissa is the double logarithmic curve table of bacterium amount (individual/ml water), see accompanying drawing 1, indicator becomes the xanchromatic time from blueness and writes down, directly find the bacterium amount from coordinate, for example the colour-change required time is 6 hours, tables look-up to such an extent that the bacterium amount is 10 ⁵/ ml water, during room temperature, than 1 ℃ of the every decline of 35C, the reaction times prolongs 0.1 times.Re-use the control medicine after finding the bacterium amount.

The present invention compared with prior art has the following advantages: existing technology does not show vibriosis bacterium quantity, the present invention can show the lonely bacterium quantity in the prawn pool, for the lonely bacterium counting of ecological regulation and control provides foundation, effectively control the infection of the vibriosis poison in the growth course of prawn.

Claims

1, a kind of prescription and production technique of prawn vibriosis ecological regulation and control vibrio counting agent, it is characterized in that lonely bacterium counting indicator by nutrition part, biostimulation agent part, reaction substrate partly, suppress part, indicating section is formed, their weight proportion is as follows respectively

A, nutrition partly are by poly-peptone, tryptone, mix, and poly-peptone accounts for 60-70% in all wts, and tryptone accounts for 30-40%,

B, biostimulation agent partly are to pulverize drying by genseng to get pale yellow mixture,

C, reaction substrate are mixed by N.F,USP MANNITOL 60-70%, inositol 30-40% and form,

D, inhibition part are to mix generation by Prostaphlin 50-60%, kantlex by 40-50% by weight ratio,

E, indicating section are to be pulverized by weight ratio acid fuchsin 40-60% to mix 400 mesh sieves, and bromothymol blue 30-50% is crushed into powder jointly, cross 400 mesh sieves and generate,

Nutrition part 70-99%

Biostimulation part 0.5-10%

Reaction substrate 0.1-5%

Inhibitor part 0.5-10%

Indicating section 0.5-5%

2, according to the prescription and the production technique of the described prawn vibriosis of claim 1 ecological regulation and control vibrio counting agent, it is characterized in that nutrition partly is to be mixed by tryptone, poly-peptone, lactoalbumin hydrolysate, yeast extract powder, poly-peptone accounts for 40-60% in all wts, all the other respectively account for all weight percents and are respectively, and tryptone accounts for that 20-30%, lactoalbumin hydrolysate account for 10-20%, yeast extract accounts for 5-10%.

3, according to the prescription and the production technique of the described prawn vibriosis of claim 1 ecological regulation and control vibrio counting agent, it is characterized in that the biostimulation agent partly is with genseng, northern stilbene, Radix Codonopsis, they respectively account for weight percent and are respectively genseng 40-70%, northern stilbene 20-30%, Radix Codonopsis 10-30%, pulverized 200 mesh sieves after weighing jointly, the 2-4 times of organic solvent extraction that adds above-mentioned weight, after four extractions, add 0.05-1% medical active decolouring 2 times, be lower than the following temperature decompression of four degrees celsius, drying gets pale yellow mixture, adds sodium sulphite anhydrous 99.3 10-30% mixing and mixes all.

4,, it is characterized in that reaction substrate is mixed and formed by N.F,USP MANNITOL 20-40%, glucose 20-40%, inositol 10-30%, trisodium citrate 5-10% according to the prescription and the production technique of the described prawn vibriosis of claim 1 ecological regulation and control vibrio counting agent.

5, according to the prescription and the production technique of the described prawn vibriosis of claim 1 ecological regulation and control vibrio counting agent, it is characterized in that suppressing part is to pulverize 400 mesh sieves by the Viola crystallina of 1.5-10% and Totazina, Prostaphlin, penicillin, kantlex respectively account for 2.5-10%, ametycin 0.5-10%, inositol 50-70% mixes generation by weight ratio.

6, according to the prescription and the production technique of the described prawn vibriosis of claim 1 ecological regulation and control vibrio counting agent, its feature is to be respectively toluylene red 5-10%, rhodizonic acid 1.5-10%, acid fuchsin 30-50% by weight ratio to pulverize and mixed 400 mesh sieves at indicating section, thymol blue 5-20%, bromothymol blue 5-20%, anhydrous sodium carbonate 5-10% is crushed into powder jointly.